Causes of maize density loss in farmers' fields in Northeast China

Increasing plant density is an effective and important way to reduce maize yield gaps in Northeast China. However, the fact is that a significant plant density gap exists between optimum plant density and actual plant density in farmers' fields.To quantify the density gap between planned planting density and final harvest plant density(HPD), we studied 60 farmers' fields on three types of soil for three crop seasons from 2015 to 2017 by measuring their plant-plant distance, actual seedlings density(ASD), final HPD and yield. We also explored the potential causes of density loss by digging the places where the seedlings were missing for two consecutive years in 2016–2017. Results show that the three-year average HPD in farmers' fields was 59 699 plants ha~(–1), which was significantly lower than the planned density, including both the machine setting density(MSD; 67 962 plants ha~(–1)) and theoretical plant density(TPD; 67 467 plants ha~(–1)). No significant difference was found in HPD between years and soil types. However, for MSD and TPD, the average value in 2015 was significantly higher than that in 2016 and 2017. No significant difference between soil types was observed. Furthermore,the results from 2016 till 2017 indicated that a lack of seeds in the soil, a failure to germinate due to low-quality seeds,and a lack of seedlings breaking out of the soil due to environmental problems explained approximately 60.88, 10.33 and 28.80% of density loss, respectively. According to our survey, 63% of farmers did not know their own TPD and HPD, and 54% of farmers did not know the density loss. Therefore, we argue that farmers' limited knowledge of density and density loss is an urgent problem that needs to be solved in maize production. These observations will be useful for determining best management practices for maize production and for providing helpful suggestions for machine improvement.     

QTL consistency for agronomic traits across three generations and potential applications in popcorn

Favorable agronomic traits are important to improve productivity of popcorn. In this study, a recombinant inbred line(RIL) population consisting of 258 lines was evaluated to identify quantitative trait loci(QTLs) for nine agronomic traits(plant height, ear height, top height(plant height subtracted ear height), top height/plant height, number of leaves above the top ear, leaf area, stalk diameter, number of tassel branches and the length of tassel) under three environments. Meta-analysis was conducted then to integrate QTLs identified across three generations(RIL, F2:3 and BC2F2) developed from the same crosses. In total, 179 QTLs and 36 meta-QTLs(m QTL) were identified. The percentage of phenotypic variation(R2) explained by any single QTL varied from 3.86 to 28.4%, and 24 QTLs with contributions over 15%. Nine common QTLs located in the same or similar chromosome regions were detected across three generations. Five meta-QTLs were identified including QTLs in three independent studies. Seven important m QTLs were composed of 11–26 QTLs for 4–7 traits, respectively. Only 11 m QTLs were commonly identified in the same or similar chromosome regions across agronomic traits, popping characteristics(popping fold, popping volume and popping rate) and grain yield components(ear weight per plant, grain weight per plant, 100-grain weight, ear length, kernel number per row, ear diameter, row number per ear and kernel ratio) by meta-QTL analysis. In conclusion, we identified a list of QTLs, some of which with much higher contributions to agronomic traits should be valuable for further study in improving both popping characteristics and grain yield components in popcorn.     

Does heat accumulation alter crop phenology,fibre yield and fibre properties of sunnhemp (Crotalaria juncea L.) genotypes with changing seasons?

Field experiments were carried out in split plot design during the dry and wet seasons for two years(two seasons each in 2016–2017 and 2017–2018) with two genotypes(SH4 and SUIN053), two plant geometry(30×15 cm and 45×15 cm main plots) and three levels of NPK(20 kg N ha~(–1), 40 kg P ha~(–1) and 40 kg K ha~(–1); 20 kg N ha~(–1), 60 kg P ha~(–1) and 60 kg K ha~(–1); 20 kg N ha~(–1), 80 kg P ha~(–1) and 80 kg K ha~(–1)) with an objective to study the relationship between fibre yield of sunhmep and thermal indices. The results indicated that the thermal units such as cumulative heat unit(CHU), photo thermal unit(PTU) and helio thermal unit(HTU) were the highest during dry seasons, while relative temperature disparity(RTD) was the highest during wet seasons irrespective of the genotypes, plant geometry and fertilizer levels. The combined analysis of variance showed that the suitability of sunnhemp genotypes for obtaining fibre and seed yields varied with season. The results further indicated that sunnhemp grew during dry seasons with longer photoperiod and higher values of growing degree days(GDD), HTU and PTU resulted in a higher fibre yield, while a higher seed yield and relatively longer, finer and stronger fibres were obtained during wet seasons with higher RTD values. Regression analysis indicated that CHU was positively related to fibre yield, while RTD was positively related to seed yield. CHU beyond 2 000 °C d reduced seed yield and favoured fibre production. In contrary to CHU, RTD values were positively related to seed yield and negatively related to fibre yield. Similarly, HTU had an inverse relationship with fibre yield while PTU had a positive relationship with fibre yield. The genotype SH4 produced a seed yield of 1 361 kg ha~(–1) during wet seasons, which was significantly higher than SUIN053, while a fibre yield of 990 kg ha~(–1)(significantly higher than that of SH4) was obtained for SUIN053 that required less CHU to attain the phenological events during dry seasons. The per unit area yields of seed and fibre with the closer spacing(30 cm×15 cm) by virtue of higher plant density were 17.0 and 14.9% higher than those with the spacing of 45 cm×15 cm, respectively. Higher doses of P and K resulted in higher seed and fibre yields.     

The effect of solar radiation change on the maize yield gap from the perspectives of dry matter accumulation and distribution

The uneven distribution of solar radiation is one of the main reasons for the variations in the yield gap between different regions in China and other countries of the world.In this study,different solar radiation levels were created by shading and the yield gaps induced by those levels were analyzed by measuring the aboveground and underground growth of maize.The experiments were conducted in Qitai,Xinjiang,China,in 2018 and 2019.The maize cultivars Xianyu 335 (XY335)and Zhengdan 958 (ZD958) were used with planting density of 12×10~4 plants ha~(–1) under either high solar radiation (HSR)or low solar radiation (LSR,70%of HSR).The results showed that variation in the solar radiation resulted in a yield gap and different cultivars behaved differently.The yield gaps of XY335 and ZD958 were 8.9 and 5.8 t ha~(–1) induced by the decreased total intercepted photosynthetically active radiation (TIPAR) of 323.1 and 403.9 MJ m~(–2) from emergence to the maturity stage,respectively.The average yield of XY335 was higher than that of ZD958 under HSR,while the average yield of ZD958 was higher than that of XY335 under LSR.The light intercepted by the canopy and the photosynthetic rates both decreased with decreasing solar radiation.The aboveground dry matter decreased by 11.1% at silking and 21% at maturity,and the dry matter of vegetative organs and reproductive organs decreased by 9.8 and 20.9%at silking and by 12.1 and 25.5% at physiological maturity,respectively.Compared to the HSR,the root weights of XY335 and ZD958 decreased by 54.6 and 45.5%,respectively,in the 0–60 cm soil layer under LSR at silking stage.The aboveground and underground growth responses to different solar radiation levels explained the difference in yield gap.Selecting suitable cultivars can increase maize yield and reduce the yield gaps induced by variation of the solar radiation levels in different regions or under climate change.     

The priority of management factors for reducing the yield gap of summer maize in the north of Huang-Huai-Hai region,China

Understanding yield potential, yield gap and the priority of management factors for reducing the yield gap in current intensive maize production is essential for meeting future food demand with the limited resources. In this study, we conducted field experiments using different planting modes, which were basic productivity(CK), farmer practice(FP), high yield and high efficiency(HH), and super high yield(SH), to estimate the yield gap. Different factorial experiments(fertilizer, planting density, hybrids, and irrigation) were also conducted to evaluate the priority of individual management factors for reducing the yield gap between the different planting modes. We found significant differences between the maize yields of different planting modes. The treatments of CK, FP, HH, and SH achieved 54.26, 58.76, 65.77, and 71.99% of the yield potential, respectively. The yield gaps between three pairs: CK and FP, FP and HH, and HH and SH, were 0.76, 1.23 and 0.85 t ha~(–1), respectively. By further analyzing the priority of management factors for reducing the yield gap between FP and HH, as well as HH and SH, we found that the priorities of the management factors(contribution rates) were plant density(13.29%)fertilizer(11.95%)hybrids(8.19%)irrigation(4%) for FP to HH, and hybrids(8.94%)plant density(4.84%)fertilizer(1.91%) for HH to SH. Therefore, increasing the planting density of FP was the key factor for decreasing the yield gap between FP and HH, while choosing hybrids with density and lodging tolerance was the key factor for decreasing the yield gap between HH and SH.     

Effect of wide-narrow row arrangement in mechanical pot-seedling transplanting and plant density on yield formation and grain quality of japonica rice

Mechanical pot-seedling transplanting is an innovatively developed transplanting method that has the potential to replace mechanical carpet-seedling transplanting. However, the initial pot-seedling transplanting machine lacked optimized density spacing and limited yield potential for japonica rice. Therefore, ascertaining the optimized density by wide-narrow rows and the appropriate transplanting method for yield formation and grain quality of japonica rice is of great importance for high-quality rice production. Field experiments were conducted using two japonica rice cultivars Nanjing 9108 and Nanjing 5055 under three transplanting methods in 2016 and 2017: mechanical pot-seedling transplanting with wide-narrow row(K, average row spacing of 30 cm); equidistant row(D, 33 cm×12 cm); and mechanical carpet-seedling transplanting(T, 30 cm×12.4 cm). In addition, five different density treatments were set in K(K1–K5, from 18.62×10~4 to 28.49×10~4 hills ha~(–1)). The results showed that the highest yield was produced by a planting density of 26.88×104 hills ha~(–1) in mechanical pot-seedling transplanting with wide-narrow row with a greater number of total spikelets that resulted from significantly more panicles per area and slightly more grain number per panicle, as compared with equidistant row, and yield among density in wide-narrow row showed a parabolic trend. Compared with mechanical carpet-seedling transplanting, the treatment of the highest yield increased yield significantly, which was mainly attributed to the larger sink size with improved filled-grain percentage and grain weight, higher harvest index, and increased total dry matter accumulation, especially the larger amount accumulated from heading stage to maturity stage. With the density in wide-narrow row decreasing, processing quality, appearance quality, and nutrition quality were all improved, whereas amylose content and the taste value were decreased. Compared with mechanical carpet-seedling transplanting, mechanical pot-seedling transplanting improved processing quality and nutrition quality, but decreased amylose content and deteriorated appearance quality. These results suggested that mechanical pot-seedling transplanting with wide-narrow row coupling produced a suitable planting density of 26.88×10~4 hills ha~(–1) and may be an alternative approach to improving grain yield and quality for japonica rice.     

Effects of different agricultural treatments on narrowing winter wheat yield gap and nitrogen use efficiency in China

Under the limited cultivated land area and the pursuit of sustainable agricultural development,it is essential for the safety of grain production to study agricultural management approaches on narrowing the winter wheat yield gap and improving nitrogen use efficiency (NUE) in China.In this study,DSSAT-CERES-Wheat Model is used to simulate winter wheat yield under different agricultural treatments,and we analyze yield gaps and NUE with different management scenarios at regional scales and evaluate the suitable approaches for reducing yield gap and increasing NUE.The results show that,the potential of narrowing yield gap ranges 300–900 kg ha~(–1) with soil nutrients increase,400–1 200 kg ha~(–1) with sowing date adjustment and 0–400 kg ha~(–1) with planting density increase as well as 700–2 200 kg ha~(–1) with adding nitrogen fertilizer.Contribution rates of management measures of soil nutrients,sowing date adjusting,planting density,and nitrogen fertilizers are 5–15%,5–15%,0–4%,and 10–20%,respectively.Difference in nitrogen partial productivity ranges 3–10 kg kg~(–1) for soil nutrients,1–10 kg kg~(–1) for sowing date adjusting,1–5 kg kg~(–1) for planting density increase,and–12–0 kg kg~(–1) for adding nitrogen fertilizers,respectively.It indicates that four treatments can narrow yield gap and improve the NUE in varying degrees,but increasing nitrogen fertilizer leads to the decrease of NUE.     

Post-silking nitrogen accumulation and remobilization are associated with green leaf persistence and plant density in maize

Stay green(SG) maize was found to have higher grain yield and post-silking nitrogen(N) uptake(PostN) compared with a non-stay green(NSG) hybrid. To understand the effects of plant density on grain yield(GY) and N efficiency in modern maize hybrids, we compared two modern hybrids(SG hybrid DY508 and NSG hybrid NH101) with similar maturity ratings at three plant densities(45 000, 60 000, and 75 000 pl ha~(–1)) in 2014 and 2015. GY, leaf senescence, dry matter(DM) accumulation,N accumulation, PostN, and post-silking N remobilization(RemN) were analyzed. DY508 and NH101 had similar GY, but DY508 had higher thousand kernel weight(TKW) and lower kernel number(KN) than NH101. Plant density significantly increased GY in the two hybrids. On average, over the two years, plant density improved GY in DY508 and NH101 by 18.5 and 11.1%, respectively, but there were no differences in total dry matter(TDM) and post-silking DM(PostDM) between the two hybrids. Plant density improved leaf N, stem N, and grain N at the silking and maturity stages in 2014 and 2015. DY508 was lower in harvest index(HI), nitrogen harvest index(NHI), and grain N concentration(GNC) than NH101. Grain N in DY508 was 2.61 kg ha~(–1) less than in NH101, and this was caused by lower GNC and leaf RemN. On the average, DY508 was 1.62 kg ha~(–1) less in leaf remobilized N(leaf RemN) than NH101, but was similar in stem remobilized N(stem RemN;2.47 kg ha~(–1) vs. 3.41 kg ha~(–1)). Maize hybrid DY508 shows delayed leaf senescence in the upper and bottom canopy layers in the later stages of growth. The present study provides evidence that the NH101, which has rapid leaf senescence at the late grain-filling stage, has gained equivalent GY and higher leaf RemN, and was more efficient in N utilization.     

Increased plant density and reduced N rate lead to more grain yield and higher resource utilization in summer maize

Planting at an optimum density and supplying adequate nitrogen(N) to achieve higher yields is a common practice in crop production, especially for maize(Zea mays L.); however, excessive N fertilizer supply in maize production results in reduced N use efficiency(NUE) and severe negative impacts on the environment. This research was conducted to determine the effects of increased plant density and reduced N rate on grain yield, total N uptake, NUE, leaf area index(LAI), intercepted photosynthetically active radiation(IPAR), and resource use efficiency in maize. Field experiments were conducted using a popular maize hybrid Zhengdan 958(ZD958) under different combinations of plant densities and N rates to determine an effective approach for maize production with high yield and high resource use efficiency. Increasing plant density was clearly able to promote N absorption and LAI during the entire growth stage, which allowed high total N uptake and interception of radiation to achieve high dry matter accumulation(DMA), grain yield, NUE, and radiation use efficiency(RUE). However, with an increase in plant density, the demand of N increased along with grain yield. Increasing N rate can significantly increase the DMA, grain yield, LAI, IPAR, and RUE. However, this increase was non-linear and due to the input of too much N fertilizers, the efficiency of N use at NCK(320 kg ha~(–1)) was low. An appropriate reduction in N rate can therefore lead to higher NUE despite a slight loss in grain production. Taking into account both the need for high grain yield and resource use efficiency, a 30% reduction in N supply, and an increase in plant density of 3 plants m~(–2), compared to LD(5.25 plants m~(–2)), would lead to an optimal balance between yield and resource use efficiency.     

Effects of plant density and mepiquat chloride application on cotton boll setting in wheat–cotton double cropping system

Sowing cotton directly after harvesting wheat in the Yangtze River Valley of China requires early mature of cotton without yield reduction. Boll-setting period synchronisation and more yield bolls distributed at the upper and middle canopy layers are also required for harvesting. The objective of this study is to quantify the individual and interaction effects of plant density and plant growth regulator mepiquat chloride(MC) on temporal and spatial distributions of yield bolls, as well as yield and yield components. During the 2013–2016 cotton growing seasons, the experiments were conducted on a shortseason cotton cultivar CRRI50 at Yangzhou University, China. Various combinations of plant density(12.0, 13.5 and 15.0 plants m~(–2)) and MC dose(180, 270 and 360 g ha~(–1)) were applied on cotton plants. The combination of 13.5 plants m~(–2) and 270 g ha~(–1) MC resulted in the greatest boll number per unit area, the highest daily boll setting number and more than 90% of bolls positioned within 45–80 cm above the ground. In conclusion, appropriate MC dose in combination of high plant density could synchronize boll-setting period and retain more bolls at the upper and middle canopy layers without yield reduction in the system of direct-seeded cotton after wheat harvest, and thus overcome the labor-intensive problem in current transplanting cropping system.     

Effects of nitrogen application rate and hill density on rice yield and nitrogen utilization in sodic saline–alkaline paddy fields

Soil salinity and alkalinity can inhibit crop growth and reduce yield,and this has become a global environmental concern.Combined changes in nitrogen (N) application and hill density can improve rice yields in sodic saline–alkaline paddy fields and protect the environment.We investigated the interactive effects of N application rate and hill density on rice yield and N accumulation,translocation and utilization in two field experiments during 2018 and 2019 in sodic saline–alkaline paddy fields.Five N application rates (0 (control),90,120,150,and 180 kg N ha~(-1) (N0–N4),respectively) and three hill densities(achieved by altering the distance between hills,in rows spaced 30 cm apart:16.5 cm (D1),13.3 cm (D2) and 10 cm (D3))were utilized in a split-plot design with three replicates.Nitrogen application rate and hill density significantly affected grain yield.The mathematical model of quadratic saturated D-optimal design showed that with an N application rate in the range of 0–180 kg N ha~(-1),the highest yield was obtained at 142.61 kg N ha~(-1) which matched with a planting density of 33.3×10~4 ha~(-1).Higher grain yield was mainly attributed to the increase in panicles m~(–2).Nitrogen application rate and hill density significantly affected N accumulation in the aboveground parts of rice plants and showed a highly significant positive correlation with grain yield at maturity.From full heading to maturity,the average N loss rate of the aboveground parts of rice plants in N4 was 70.21% higher than that of N3.This is one of the reasons why the yield of N4 treatment is lower than that of the N3 treatment.Nitrogen accumulation rates in the aboveground parts under treatment N3 (150 kg N ha~(-1)) were 81.68 and 106.07% higher in 2018 and 2019,respectively,than those in the control.The N translocation and N translocation contribution rates increased with the increase in the N application rate and hill density,whereas N productivity of dry matter and grain first increased and then decreased with the increase in N application rate and hill density.Agronomic N-use efficiency decreased with an increase in N application rate,whereas hill density did not significantly affect it.Nitrogen productivity of dry matter and grain,and agronomic N-use efficiency,were negatively correlated with grain yield.Thus,rice yield in sodic saline–alkaline paddy fields can be improved by combined changes in the N application rate and hill density to promote aboveground N accumulation.Our study provides novel evidence regarding optimal N application rates and hill densities for sodic saline–alkaline rice paddies.     

Soil tillage methods by years interaction for dry matter of plant yield of maize (Zea mays L.) using additive main effects and multiplicative interaction model

The objective of this study was to assess soil tillage methods by years interaction for dry matter of plant yield of maize(Zea mays L.) grown in West Poland by the additive main effects and multiplicative interaction model. The study comprised four soil tillage methods, analysed in 12 years through field trials arranged in a randomized complete block design, with four replicates. Dry matter of plant yield of the tested soil tillage methods varied from 86.7 dt ha~(–1)(for no-plough tillage in 2005) to 246.4 dt ha~(–1)(for complete conventional tillage in 2012), with an average of 146.6 dt ha~(–1). In the variance analysis, 49.07% of the total dry matter of plant yield variation was explained by years, 12.69% by differences between soil tillage methods, and 10.53% by soil tillage methods by years interaction. Dry matter of plant yield is highly influenced by soil tillage methods by years factors.     

Changes in soil organic carbon pools following long-term fertilization under a rain-fed cropping system in the Loess Plateau,China

Understanding the mechanism of soil organic carbon(SOC) sequestration is of paramount importance in sustaining crop productivity and mitigating climate change. Long-term trials were employed to investigate the responses of total SOC and its pools, i.e., mineral-associated OC(MOC), particulate OC(POC, containing Light-POC and Heavy-POC), to fertilization regimes at Yangling(25-year), Tianshui(35-year) and Pingliang(37-year) under a rain-fed cropping system in the Loess Plateau. The fertilization regimes in each trial included three treatments, i.e., control(no nutrient input, CK), chemical fertilizers(CF), and organic manure plus chemical fertilizers(MCF). Relative to the CK, long-term fertilization appreciably increased SOC storage by 134, 89 and 129 kg ha~(–1) yr~(–1) under CF, and 418, 153 and 384 kg ha~(–1) yr~(–1) under MCF in plough layer soils(0–20 cm), respectively, at the Yangling, Tianshui and Pingliang sites. The MOC pools accounted for 72, 67 and 64% of the total SOC at the above three sites with sequestration rates of 76, 57 and 83 kg ha~(–1) yr~(–1) under CF and 238, 118 and 156 kg ha~(–1) yr~(–1) under MCF, respectively. Moreover, the MOC pool displayed a saturation behavior under MCF conditions. The POC accordingly constituted 27, 33 and 36% of SOC, of which Light-POC accounted for 11, 17 and 22% and Heavy-POC for 17, 16 and 15% of SOC, respectively. The sequestration rates of POC were 58, 32 and 46 kg ha~(–1) yr~(–1) under CF, and 181, 90 and 228 kg ha~(–1) yr~(–1) under MCF at the three respective sites, in which Light-POC explained 59, 81 and 72% of POC under CF, and 60, 40 and 69% of POC under MCF, with Heavy-POC accounting for the balance. Compared with CK, the application of CF alone did not affect the proportions of MOC or total POC to SOC, whereas MCF application markedly reduced the proportion of MOC and increased the POC ratio, mainly in the Light-POC pool. The distribution of SOC among different pools was closely related to the distribution and stability of aggregates. The present study confirmed that organic manure amendment not only sequestered more SOC but also significantly altered the composition of SOC, thus improving SOC quality, which is possibly related to the SOC saturation level.     

Effects of nitrogen fertilizer and chemical regulation on spring maize lodging characteristics,grain filling and yield formation under high planting density in Heilongjiang Province,China

Now,lodging is a major constraint factor contributing to yield loss of maize (Zea mays L.) under high planting density.Chemical regulation and nitrogen fertilizer could effectively coordinate the relationship between stem lodging and maize yield,which significantly reduce lodging and improve the grain yield.The purpose of this study was to explore the effects of chemical regulation and different nitrogen application rates on lodging characteristics,grain filling and yield of maize under high density.For this,we established a field study during 2017 and 2018 growing seasons,with three nitrogen levels of N100 (100 kg ha~(–1)),N200 (200 kg ha~(–1)) and N300 (300 kg ha~(–1)) at high planting density (90 000 plants ha~(–1)),and applied plant growth regulator (Yuhuangjin,the mixture of 3% DTA-6 and 27% ethephon) at the 7th leaf.The results showed that chemical control increased the activities of phenylalanine ammonia-lyase (PAL),tyrosine ammonia-lyase (TAL),4-coumarate:Co A ligase (4CL),and cinnamyl alcohol dehydrogenase (CAD),and increased the lignin,cellulose and hemicellulose contents at the bottom of the 3rd internode,which significantly reduced the lodging percentage.The lignin-related enzyme activities,lignin,cellulose and hemicellulose contents decreased with the increase of nitrogen fertilizer,which significantly increased the lodging percentage.The 200 kg ha~(–1) nitrogen application and chemical control increased the number,diameter,angle,volume,and dry weight of brace roots.The 200 kg ha~(–1) nitrogen application and chemical control significantly increased the activities of ADP-glucose pyrophosphorylase (AGPase),soluble starch synthase (SSS) and starch branching enzyme(SBE),which promoted the starch accumulation in grains.Additional,improved the maximum grain filling rate (V_(max)) and mean grain filling rate (V_m),which promoted the grain filling process,significantly increased grain weight and grain number per ear,thus increased the final yield.     

Does nitrogen application rate affect the moisture content of corn grains?

Nitrogen fertilizer application is an important measure to obtain high and stable corn yield, and the moisture content of corn grains is an important factor affecting the quality of mechanical grain harvesting. In this study, four different nitrogen fertilizer treatments from 0 to 450 kg ha~(–1) pure nitrogen were set for a planting density of 12.0×10~4 plants ha~(–1) in 2017 and 2018, and 18 different nitrogen fertilizer treatments from 0 to 765 kg ha~(–1) pure nitrogen were set for planting densities of 7.5×10~4 and 12.0×10~4 plants ha~(–1) in 2019, to investigate the effect of nitrogen application rate on the moisture content of corn grains. Under each treatment, the growth of corn, leaf area index(LAI) of green leaves, grain moisture content, and grain dehydration rate were measured. The results showed that, as nitrogen application increased from 0 to 765 kg ha~(–1), the silking stage was delayed by about 1 day, the maturity stage was delayed by about 1–2 days, and the number of physiologically mature green leaves and LAI increased. At and after physiological maturity, the extreme difference in grain moisture content between different nitrogen application rates was 1.9–4.0%. As the amount of nitrogen application increased, the corn grain dehydration rate after physiological maturity decreased, but it did not reach statistical significance between nitrogen application rate and grain dehydration rate. No significant correlation was observed between LAI at physiological maturity and grain dehydration rate after physiological maturity. In short, nitrogen application affected the grain moisture content of corn at and after physiological maturity, however, the difference in grain moisture content among different nitrogen application rates was small. These results suggest that the effect of nitrogen application on the moisture content of corn grains should not be considered in agricultural production.     

Reducing maize yield gap by matching plant density and solar radiation

Yield gap exists because the current attained actual grain yield cannot yet achieve the estimated yield potential. Chinese high yield maize belt has a wide span from east to west which results in different solar radiations between different regions and thus different grain yields. We used multi-site experimental data, surveyed farmer yield data, the highest recorded yield data in the literatures, and simulations with Hybrid-Maize Model to assess the yield gap and tried to reduce the yield gap by matching the solar radiation and plant density. The maize belt was divided into five regions from east to west according to distribution of accumulated solar radiation. The results showed that there were more than 5.8 Mg ha~(–1) yield gaps between surveyed farmer yield and the yield potential in different regions of China from east to west, which just achieved less than 65% of the yield potential. By analyzing the multi-site density experimental data, we found that the accumulated solar radiation was significantly correlated to optimum plant density which is the density with the highest yield in the multi-site density experiment(y=0.09895 x–32.49, P0.01), according to which the optimum plant densities in different regions from east to west were calculated. It showed that the optimum plant density could be increased by 60.0, 55.2, 47.3, 84.8, and 59.6% compared to the actual density, the grain yield could be increased by 20.2, 18.3, 10.9, 18.1, and 15.3% through increasing plant density, which could reduce the yield gaps of 33.7, 23.0, 13.4, 17.3, and 10.4% in R(region)-1, R-2, R-3, R-4, and R-5, respectively. This study indicates that matching maize plant density and solar radiation is an effective approach to reduce yield gaps in different regions of China.     

Determination of optimum nitrogen application rates in Zhejiang Province,China, based on rice yields and ecological security

Excessive nitrogen(N) fertilization in intensive agricultural areas such as the plain region of South China has resulted in low nitrogen use efficiency and serious environmental problems. To determine the optimum N application rate, grain yield, apparent nitrogen recovery efficiency(ANRE), apparent N loss, and ammonium(NH_3) volatilization under different N application rates in the three years from 2012 to 2014 were studied. The results showed that the relationship between grain yields and N application rate in the three years were well fitted by quadratic equations. When N application rate reached 197 kg ha~(–1) in 2012, 199 kg ha~(–1) in 2013 and 196 kg ha~(–1) in 2014, the plateau of the grain yields appeared. With the increase of N application rate, the ANRE for rice decreased which could be expressed with sigmoidal equation; when N application rate was 305 kg ha~(–1) in 2012, 275 kg ha~(–1) in 2013 and 312 kg ha~(–1) in 2014, the curves of ANRE appeared turing points. Besides, the relationship between soil Nresidual and N application rate was fitted by the quadratic equation and the maximums of soil Nresidual were reached in the three years with the N application rate of 206, 244 and 170 kg ha~(–1), respectively. Statistical analysis indicated that NH3 volatilization and apparent N loss in three years all increased with the increasing N application rate. When the amount of NH3 volatilization increased to 11.6 kg N ha~(–1) in 2012, 40.5 kg N ha~(–1) in 2013 and 57.0 kg N ha~(–1)in 2014, the apparent N loss in the three years had obvious increase. To determine the optimum N application rate, the average N application on the plateau of the grain yield was considered as the lower limit while the average N application rate at the turning points of ANRE, the residual N in soil and apparent N loss was taken as the upper limit. According to the results in three years, the optimum N application rate for rice in Zhejiang was 197–255 kg ha~(–1).     

Heterotic loci identified for plant height and ear height using two CSSLs test populations in maize

Heterosis is an important biological phenomenon, and it has been used to increase grain yield, quality and resistance to abiotic and biotic stresses in many crops. However, the genetic mechanism of heterosis remains unclear up to now. In this study, a set of 184 chromosome segment substitution lines(CSSLs) population, which derived from two inbred lines lx9801(the recurrent parent) and Chang 72(the donor parent), were used as basal material to construct two test populations with the inbred lines Zheng 58 and Xun 9058. The two test populations were evaluated in two locations over two years, and the heterotic loci for plant height and ear height were identified by comparing the performance of each test hybrid with the corresponding CK at P0.05 significant level using one-way ANOVA analysis and Duncan's multiple comparisons. There were 24 and 29 different heterotic loci(HL) identified for plant height and ear height in the two populations at two locations over two years. Three HL(hlPH4a, hlPH7c, hlPH1b) for plant height and three(hlEH1d, hlEH6b, hlEH1b) for ear height were identified in the CSSLs×Zheng 58 and CSSLs×Xun 9058 populations as contributing highly to heterosis performance of plant height and ear height across four environments. Among the 29 HL identified for ear height, 12 HL(41.4%) shared the same chromosomal region associated with the HL(50.0%) identified for plant height in the same test population and environment.     

Effects of silicon amendment on the occurrence of rice insect pests and diseases in a field test

Rice is one of the most important staple foods for the world population,but it is attacked by a number of destructive pests.While evidence from greenhouse and laboratory tests has shown that silicon(Si)amendment can confer enhanced resistance to pests in rice,few studies have directly demonstrated the Si-mediated protection from pests in a field situation.In this study,field plots with silicon amendments at 0,75,150 and 300 kg SiO_2 ha~(–1) in early-and late-season rice were employed to evaluate the effects of silicon amendment on the occurrence of major insect pests and diseases and rice yield.Compared with the control plots without silicon amendment,plant damage by stem borer and leaf folder and population size of planthopper were significantly lower in three to five of the seven monitoring observations in each season in the plots amended with 300 kg SiO_2 ha~(–1).The disease index of rice blast in the early-season rice was lower in the plots amended with Si at 300 kg SiO_2 ha~(–1) than in the control plots,while Si protection from rice blast in the late-season rice and from rice sheath blight in the early-season rice were not apparent.An insignificant increase of rice yield by 16.4%(604 kg ha~(–1))was observed in the plots amended with 300 kg SiO_2 ha~(–1) over the control plots.Our results indicate that Si amendment at 300kg SiO_(2 )ha~(–1) can provide substantial protection from some of the rice pests under field conditions.These findings support the recommendation of silicon amendment as a key component of integrated management of rice pests.     

Interaction effect of nitrogen form and planting density on plant growth and nutrient uptake in maize seedlings

High planting density is essential to increasing maize grain yield.However,single plants suffer from insufficient light under high planting density.Ammonium(NH_4~+)assimilation consumes less energy converted from radiation than nitrateIt is hypothesized that a mixed NO_3~–/NH_4~+supply is more important to improving plant growth and population productivity under high vs.low planting density.Maize plants were grown under hydroponic conditions at two planting densities(low density:only).A significant interaction effect was found between planting density and N form on plant biomass.Compared to nitrate only,75/25NO_3~–/NH_4~+increased per-plant biomass by 44%under low density,but by 81%under high density.Treatment with 75/25NO_3~–/NH_4~+increased plant ATP,photosynthetic rate,and carbon amount per plant by 31,7,and 44%under low density,respectively,but by 51,23,and 95%under high density.Accordingly,carbon level per plant under 75/25NO_3~–/NH_4~+was improved,which increased leaf area,specific leaf weight and total root length,especially for high planting density,increased by 57,17 and 63%,respectively.Furthermore,under low density,75/25NO_3~–/NH_4~+increased nitrogen uptake rate,while under high density,75/25NO_3~–/NH_4~+increased nitrogen,phosphorus,copper and iron uptake rates.By increasing energy use efficiency,an optimum NO_3~–/NH_4~+ratio can improve plant growth and nutrient uptake efficiency,especially under high planting density.In summary,an appropriate supply of NH_4~+in addition to nitrate can greatly improve plant growth and promote population productivity of maize under high planting density,and therefore a mixed N form is recommended for high-yielding maize management in the field.