首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 93 毫秒
1.
2.
Hexokinase Ⅱ has been demonstrated to play the role of a key enzyme member in the glycolysis reaction. It catalyzes the conversion of glucose to glucose-6-phosphate, thus committing glucose to the glycolytic pathway. In this paper, the partial exons and introns 10, 11, 13 and 14 of the porcine HK2 gene were cloned and sequenced by comparative genomics. Comparative sequencing of three pig breeds revealed ten putative single-nucleotide polymorphisms (SNPs), one of which in intron 10 with differing bases (G981A) is within the restriction site for enzyme Msp I. Distribution of Msp I -RFLP genotype and allele frequencies among different pig breeds were studied. By association analysis between Msp I PCR- RFLP polymorphism (AA, AB, BB genotypes of HK2 gene intron 10) and some meat quality and carcass traits in F2 group, which was constructed by our laboratory, a significant difference of pig average backfat at rump was found between AB and BB genotypes (P〈0 05) in F2 group. In addition, the pattern of expression of ilK2 in a variety of tissues in pig was also determined using semi-quantitative RT-PCR. The expression of HK2 mRNA was detected only in pig skeletal muscle.  相似文献   

3.
4.
Starch branching enzyme (SBE) catalyzes the biosynthesis of amylopectin. We described the isolation and characterization of SBEIIb promoter and their expression patterns in transgenic tobacco. Using the genomic DNA of maize cultivar Lunuo 1 as template, the SBEIIb promoter was isolated by PCR and was cloned into pMD18-T vector. To study SEBIIb gene regulation at the cellular level, SBEIIb promoter was fused to the ~-glucuronidase (GUS) report gene. The results of the fluorometric GUS assays indicate that the sbeⅡb-GUS fusion directed a seed-specific expression. Four series of constructs were made with the promoter and the GUS reporter gene to investigate the cis-acting analysis, showing that the four different constructs all can drive expression of the GUS gene in seed plumule and cotyledon and the GUS activity was apparently decreased with the progressive loss of promoter 5' end.  相似文献   

5.
6.
Brassinosteroids (BRs) are an important class of plant steroidal hormones that are essential in a wide variety of physiological processes. To determine the effects of BRs on the development of cotton fibers, through screening cotton fiber EST database and contigging the candidate ESTs, a key gene (GhDWF1) involved in the upstream biosynthetic pathway of BRs was cloned from developing fibers of upland cotton (Gossypium hirsutum L.) cv. Xuzhou 142. The full length of the cloned cDNA is 1 849 bp, including a 37 bp 5'-untranslated region, an ORF of 1 692 bp, and a 120 bp 3'-untranslated region. The cDNA encodes a polypeptide of 563 amino acid residues with a predicted molecular mass of 65 kD. The deduced amino acid sequence has high homology with the BR biosynthetic enzyme, DWARF1/DIMINUTO, from rice, maize, pea, tomato, and Arabidopsis. Furthermore, the typical conserved structures, such as the transmembrane domain, the FAD- dependent oxidase domain, and the FAD-binding site, are present in the GhDWF1 protein. The Southern blot indicated that the GhDWF1 gene is a single copy in upland cotton genome. RT-PCR analysis revealed that the highest level of GhDWF1 expression was detected in 0 DPA (day post anthesis) ovule (with fibers) while the lowest level was observed in cotyledon. The GhDWF1 gene presents high expression levels in root, young stem, and fiber, especially, at the fiber developmental stage of secondary cell wall accumulation. Moreover, the expression level was higher in ovules (with fibers) of wildtype (Xuzhou 142) than in ovules of fuzzless-lintless mutant at the same developmental stages (0 and 4 DPA). The results suggest that the GhDWF1 gene plays a crucial role in fiber development.  相似文献   

7.
The aim of this study is to construct a prokaryotic expression vector of mouse Nanog gene and to express it in E. coli. A pair of primers was designed according to digestion sites in plasmid pGEX-KG and the Nanog gene sequence published by GenBank. The DNA fragment of 918 bp was amplified by polymerase chain reaction (PCR) from the pNA992 recombinant plasmid with Nanog gene, then cloned into pGEX-KG and transformed into the host E. coli strain TG Ⅰ. The sequence of the fragment was matched with the original sequence of pNA992. It indicated that fusion expression vector, pGEX-KG- Nanog, was constructed successfully. The pGEX-KG-Nanog plasmid was extracted from E. coli strain TG Ⅰ and was transformed into BL21(DE3) for expression. After induction by isopropyl-β-D-thiogalactoside (IPTG) at 37℃, the expression product of Nanog gene was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the expression condition was optimized. Nanog fusion protein was successfully expressed in the form of inclusion bodies. The molecular weight of the inclusion body was 63 kDa. Meanwhile, the optimum condition for the expression of Nanog fusion protein was induced with 0.8 mmol L^-1 IPTG for 5 h. The mouse Nanog gene was successfully expressed in E. coli, which laid a foundation for the purification of Nanog protein and for the preparation of polyclonal antibody.  相似文献   

8.
Anthocyanidin in plants, an important pigment, is of great interest to researchers, consumers, and commercial entities due to its physiological functions. Anthocyanin content and mRNA levels of anthocynin biosynthesis genes were investigated in storage root of different purple-fleshed sweet potatoes (PFSP) genotypes to understand the regulation mechanism of anthocyanin under weak light conditions. Anthocyanin content, its amount of accumulation, and the expression of CHS, DFR, F3H, GT, and ANS genes in the PFSP storage root under weak light conditions were studied. The results demonstrated that the anthocyanin content of the treatments was decreased and was obviously lower than that of the control until 30 days after shading in Ayamurasaki, while it was lower than that of the control from the beginning of shading in Jishu 18. Their accumulation rates of both treatmeants were lower than its control before 10-20 d of shading in Jishu 18, while those of Ayamurasaki weren't in their treatments. This indicated that Jishul 8 is more sensitive to light as compared to Ayamuraska. Under the different weak light conditions, mRNA levels for ibCHS, ibF3H, ibDFR, and ibANS were obviously decreased, while the expression of ibGT was increased. These results indicated that anthocyanin content was regulated by light at the mRNA levels and the enzymatic level in sweet potato. Therefore, the development dynamic response to anthocyanin content varied in different genotypes of PFSP, and mRNA levels of anthocyanin biosynthesis were inhibited under the weak light condition.  相似文献   

9.
10.
11.
12.
Laboratory and field studies were conducted on some aspects of biology and control methods of fruit stalk borer Oryctes elegans Prell., the study was conducted in date palm orchards in south Baghdad during the years 2009 and 2010. The laboratory rearing result ofO. elegans indicated that the larval stage period was very long reached 6 months, the body weight of the mature larva was 7.61 gm, the length and body circumference were 7.58 and 5.23 cm respectively. The period of pupa stage was 19 day and 2.97 gm body weight, while the length and body circumference were 3.74 and 3.21 cm respectively .While the weight of adult stage was 1.67 gm and 3.14 cm, 2.71 cm for the length and circumference body respectively. Results of hand collection larvae in palm trees during the regular annual service, showed reduction in population density of adults to 1.4 adult/trap per week compared with 3.3 and 14.0 adult/trap per week in the orchards were larvae collected partially and without hand collection by using the light traps with solar energy (Magna Traps, Russell IPM) respectively. Also, the study proved the existence of an inverse relation between the moon light and adult activity, which affected on the adult number cough in light traps. These results indicated that the hand collection of larvae works during months January, February, March and April at synchronized with the larval stage on palm trees, and use Magna Traps as an effective method to control O. elegans through IPM programs in palm orchards.  相似文献   

13.
The aim of the present experiment was to study the relationship between the distribution of relative light intensity in canopy and yield and quality of Wanmi peach. The optimum relative canopy light intensity was judged to be 36.3% for high quality peaches, when canopy volumes of Wanmi peach trees with a relative light intensity 〈 30% accounted for 7.7 and 47.9% of the total canopy volume in June and September, respectively. The canopy volume with a relative light intensity 〉 80% was 27.7 and 3.1% of the total canopy volume in June and September, respectively. Peach canopies were divided into 0.5 m × 0.5 m × 0.5 m cubes, with the relative light intensity being measured at different positions of the canopy during the growing season. Yield and fruit quality were also measured at these positions at harvest. The results showed that the relative light intensity decreased gradually from outside to inside and from top to bottom of the tree canopy. Fruit were mainly distributed in the upper and middle portions of the canopy, 1.5-3.0 m above ground. Regression results showed that single fruit weight and soluble solid content were positively related to relative light intensity.  相似文献   

14.
Cellular retinoic acid-binding protein 1 (CRABP1) is a well-conserved member of cytosolic lipid-binding protein family. It is an important modulator of retinoic acid signaling. Long serial analysis of gene expression (LongSAGE) analysis suggested that CRABP1 gene was differentially expressed during prenatal skeletal muscle development in porcine. Here, we obtained the full-length coding region sequence and genomic sequence of the porcine CRABP1 gene and analyzed its genomic structures. Subsequently, we examined CRABP1 chromosome assignment using INRA-University of Minnesota 7 000 porcine radiation hybrid panel (IMpRH) and explored its tissue distribution in adult Tongcheng pigs and dynamical expression profiles in prenatal skeletal muscle (33, 65 and 90 days post coitus, dpc) from Landrace (lean-type) (described as L33, L65 and L90) and Tongcheng pigs (obese-type) (described as T33, T65 and T90). The CRABPI gene was mapped to chromosome 7ql 1-q23 and closely linked to the microsatellite marker SWR1928. Quantitative real-time PCR showed that CRABP1 mRNA was highly expressed in lung and stomach, moderately expressed in placenta and uterus, and weakly expressed in other tissues. Moreover, CRABP1 gene was down-regulated during prenatal skeletal muscle development in both Landrace and Tongcheng pigs and it was expressed much higher in T33 than L33. Two single-nucleotide polymorphisms (SNPs) were detected by sequencing and mass spectrometry methods, allele frequency analysis indicated that g. 281 (G〉A) and g. 2992 (G〉A)were deviated from Hardy-Weinberg equilibrium in the Landrace and DLY (Duroc×(Landrace×Yorkshire)) pig breeds.  相似文献   

15.
Stress-associated protein (SAP) has functions in maintaining plant cell elongation, embryo development and response to abiotic stresses. TaSAP1-A1, one of the Triticum aestivum SAP1 (TaSAP1) members located on wheat chromosome 7A was isolated for polymorphism analysis. HaplII of TaSAP1-A1 was found significantly associated with thousand-grain weight (TGW) in multiple environments. In this study, HaplII also made a positive contribution to TGW in Population 2. The distribution of TaSAP1-A1 HapIII was tracked among varieties released in different years and geographical environments of China. The frequency of HapIII showed an increasing trend during the breeding process in two different populations. The ItapIII was gradually selected and applied from 6.36% in landraces to 13.50% in modem varieties. These results exhibited that TaSAP1-A1 HapIII was positively selected during wheat breeding, which is beneficial for grain-yield improvement. The preferred HapIIl was initially selected and applied in the higher latitude areas of China in accord with the long day season and longer grain filling stage in these areas. Moreover, the frequency of HaplII in recent modem varieties was still quite low (19.29-26.67%). It indicated a high application potential of TaSAP1-A 1 HapIII for improving grain yield in wheat breeding.  相似文献   

16.
《农业科学学报》2014,13(5):1024-1035
Isoprenoids are a functionally and structurally diverse class of natural organic chemicals. The universal precursors of all isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate are synthesized through the mevalonate and 2C-methyl-D-erythritol 4-phosphate (MEP) pathways, respectively. Many isoprenoids produced through the MEP pathway play an important role in plant acclimation to different light environments. Eupatorium adenophorum, an invasive weed in China, presents a remarkable capacity to acclimate to various light environments, which constitutes its solid foundation of being a successful invasive species. Thus we aimed at gaining a deeper insight into the regulation of MEP pathway in E. adenophorum to further understand the invasive mechanism. 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF or MCS) is an essential enzyme in the MEP pathway. In this paper, a novel IspF gene was cloned and characterized from E. adenophorum. Tissue-specific expression assays revealed a higher expression of EaIspF1 in leaves than in stems and roots. The expression of EaIspF1 was responsive to different light conditions. Some up-regulation of EaIspF1 expression was also found after the treatments with signal compounds and after wounding stress. Interestingly, the over-expression of EaIspF1 in Arabidopsis led to increase carotenoids contents, resulting in an enhanced tolerance to high light. Taken together, these results indicate that the EaIspF1-derived enzyme participates in isoprenoid metabolism and among others, the expression of this gene in E. adenophorum is involved in the regulation of plastidial isoprenoids, which play an important role in acclimation to various light environments.  相似文献   

17.
The study area is located in Duhok and Sulaimania province in Kurdistan region, lraq. Study soils developed from parent material that has derived from limestone. Randomize, composed and disturbed surface soil samples were collected. The bulk soils were air dried, crushed and passed through 2 mm sieve. Standard methods were used for chemical, physical, geotechnical and mineralogical analyses of soil samples. The results indicated that the study soils texture were clay to loam clay, this texture was considered as suitable for ceramic and pottery industries as a result of increasing clay contents that ranged between 301 g/kg and 676 g/kg. Soil consistence depending on geotechnical properties increased the ability of study soils for resistance rapture and deformation. Existence the high amount of cementing agents in study soils such as organic matter, iron oxides and particularly total carbonate (247.2-308.8 g/kg) act to reduce the bad effect of the smectite minerals group (high shrinkage) in soils of study locations through increasing the resistance of these soils for rupture and deformation. The existing of Kaolinite, palygorskite and chlorite allows clay to be dried in ceramic and pottery industries without cracking from shrinkage. Study soils were different in their colors as a result of existence, different pigmentation materials that led to coloring soils with different colors in turn caused coloring of pottery and ceramic materials. Since, there are no available academic studies or researches about this subject in Kurdistan region in addition the clay pottery and ceramic sector still has a good market at the same time using this type of soils for arts, therefore, this study was conducted.  相似文献   

18.
E.  O.  Idowu 《农业科学与技术》2011,(4):472-475
The protein, carbohydrate and lipid contents of the food ingested and their absorption in the intestine ofHepsetus odoe in Ado-Ekiti Reservoir, Ekiti State, Nigeria were investigated. Samples were analysed chemically for protein, fat and carbohydrate using the official methods of analysis described by the Association of Official Analytical Chemist. The ingested food had mean total protein, mean total carbohydrate and mean total lipid of 60.2% - 8.55%, 15.4% ± 1.9% and 15.6% ±1.1% of the organic content respectively. Calculated mean total percentages absorbed were 72.4% protein, 52% carbohydrate and 51.5% lipid. The foregut had the highest percentage absorption while the hindgut had the lowest for all the nutrients. Different sizes of fish showed different dietary protein, carbohydrate and lipid contents. Absorptive capacity for these components of the food also varied with fish size.  相似文献   

19.
In Kenya almost all sugarcane grown is under rainfall conditions that are planted in different locations during both long and short rainy seasons. Effect of season, location, cultivar and spacing on yield and quality of sugarcane is not clearly understood. The research was conducted at Kibos and Mumias to determine if the yields and quality of three sugarcane cultivars were affected by season, location and row spacing. Cultivars CO 945, EAK 73-335 and KEN 83-737 were manually planted during June and September in 2011. The trial was laid out as split-split plot arranged in a randomized complete block design (RCBD) replicated three times. Data collected at harvest, 17 months after planting (MAP) including cane yields, cane yield components and cane quality. The results revealed that cane length differed significantly between June and September. Stalk population and cane yields were significantly more at Mumias compared to Kibos. Cultivar EAK 73-335 showed significantly more cane yield (t/ha) than KEN 83-737 and CO 945. Both row spacing 1.2 m and 1.5 m did not affect cane yields. Brix%, Sucrose% and Purity% differed significantly between cultivars. The relationship between cane yield and stalk population was positive and significant, which indicated stalk population is a strong determinant of cane yield. The results showed that cane yield was significantly affected by location and cane quality by cultivars. Therefore Mumias is a better zone for sugarcane cultivation for both June and September seasons compared to Kibos.  相似文献   

20.
Knowledge about crop growth processes in relation to N limitation is necessary to optimize N management in farming system. Plant-based diagnostic method, for instance nitrogen nutrition index (NNI) were used to determine the crop nitrogen status. This study determines the relationship of NNI with agronomic nitrogen use efficiency (AEN), tuber yield, radiation use efficiency (RUE) and leaf parameters including leaf area index (LAI), areal leaf N content (NJ and leaf N concentration (N0. Potatoes were grown in field at three N levels: no N (N 1), 150 kg N ha^-1 (N2), 300 kg N ha^-1 (N3). N deficiency was quantified by NNI and RUE was generally calculated by estimating of the light absorbance on leaf area. NNI was used to evaluate the N effect on tuber yield, RUE, LAI, NAL, and NL. The results showed that NNI was negatively correlated with AEN, N deficiencies (NNI〈 1) which occurred for N 1 and N2 significantly reduced LAI, NL and tuber yield; whereas the N deficiencies had a relative small effect on NAL and RUE. To remove any effect other than N on these parameters, the actual ratio to maximum values were calculated for each developmental linear relationships were obtained between NNI and tuber RUE to NNI. stage of potatoes. When the NNI ranged from 0.4 to 1, positive yield, LAI, NL, while a nonlinear regression fitted the response of  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号