共查询到19条相似文献,搜索用时 78 毫秒
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《(《农业科学与技术》)编辑部》2008,(1)
研究芨芨草的遗传多样性,探讨不同地理种群的遗传分化与地理位置及生境的关系,以期为芨芨草种质资源的开发、利用与保存提供参考。1材料与方法1.1材料试验材料采自新疆不用产地,生境资料见表1。表1新疆7个居群的生境资料居群编号采集地点经度纬度海拔∥m年平均温度∥℃年降水量 相似文献
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利用幂乘方法则模型探讨了鄂尔多斯3种不同类型草地在禁牧情况下的群落结构特征,包括物种组成、物种多样性、生物量和空间分布规律.结果表明:幂乘方法则在解析鄂尔多斯不同类型草地的空间异质性时具有很好的吻合性;羊草(Leymus chinensis)草地、芨芨草(Achnatherum splenfens)草地和油蒿(Artemisia ordosica)灌丛草地均比随机分布呈现了较强的空间异质性,群落整体的空间异质性指数表现为油蒿灌丛草地>羊草草地>芨芨草草地;群落整体的物种多样性指数为油蒿灌丛草地>羊草草地>芨芨草草地;L-样方(50cm×50cm)内的平均物种数和物种多样性指数均表现为羊草草地极显著地高于油蒿灌丛草地,油蒿灌丛草地又极显著地高于芨芨草草地(P<0.001);L-样方内的平均生物量表现为油蒿灌丛草地极显著地高于芨芨草草地(P<0.001),而羊草草地与油蒿灌丛草地以及芨芨草草地之间没有显著性差异;这3种类型的草地其L-样方内的平均生物量和物种多样性指数都随着群落整体空间异质性指数的增大而增大. 相似文献
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不同地区环境条件的分异及长期筛选导致植物种子的耐盐性存在差异,基于此,以采自甘肃、新疆、陕西和内蒙等地的芨芨草种子为研究对象,通过萌发试验探究不同浓度NaCl溶液[0.00%(对照)、0.30%、 0.60%、0.90%、1.20%和1.50%]对不同地区芨芨草种子萌发的影响。结果表明:随着NaCl溶液浓度升高,所有地区芨芨草种子发芽率、发芽势、发芽指数、简化活力指数均呈下降趋势,而胚芽长度在巴彦淖尔、榆林、张掖、天祝、平川地区呈现先增后降趋势,在其他地区呈下降趋势;胚根长度在兰州、巴彦淖尔、榆林、天祝、内蒙包头、金昌、平川、景泰地区呈现先增后降趋势,在其他地区呈下降趋势。在同一NaCl浓度下,发芽率、发芽势、发芽指数最高的种子出现在酒泉地区,分别为68.33%、62.00%、54.16,最低出现在兰州地区,分别为1.00%、0.00%、0.14;胚芽、胚根最长的种子出现在巴彦淖尔地区,分别为4.47 cm、3.47 cm,最短出现在新疆地区,均为0.00 cm;简化活力指数最大的种子出现在金昌地区(为0.38),最小出现在新疆地区(为0.00)。经综合评价发现,耐盐性最强的为酒泉地区芨芨草种子,最弱的为兰州地区芨芨草种子。多元回归分析表明,来自所有采样区的芨芨草鲜质量、发芽率、发芽指数和发芽势均对耐盐性响应较强,可以作为种子萌发期耐盐性评价核心指标。 相似文献
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[目的]研究青海湖地区4种荒漠植物的抗逆性。[方法]通过比较中麻黄、狼毒、芨芨草、黄缨菊4种植物的抗逆性指标(可溶性蛋白、MDA、游离Pro含量以及SOD、POD、CAT活性),分析青海湖地区4种植物的抗逆性,并运用Fuzzy隶属函数法对4种植物抗逆性进行综合评价。[结果]青海湖地区低温、干旱逆境下,4种荒漠植物的SOD活性表现为狼毒〉黄缨菊〉中麻黄〉芨芨草;CAT活性表现为狼毒〉黄缨菊〉芨芨草〉中麻黄,相同的高盐度环境下,狼毒的CAT活性比中麻黄要高出8倍,说明狼毒的抗盐性要强于中麻黄;游离Pro含量表现为黄缨菊〉中麻黄〉狼毒〉芨芨草,相同逆境下中麻黄的Pro含量是狼毒的2.83倍,这是由于狼毒的MDA含量最低,体内膜质过氧化伤害最小,导致胁迫渗透物质Pro含量低,也说明狼毒的抗逆性强于中麻黄。Fuzzy隶属函数综合评价表明,4种荒漠植物的抗逆性表现为黄缨菊〉狼毒〉中麻黄〉芨芨草。[结论]该研究为青海湖地区抗旱新品种的选育和优良种质资源的开发提供了理论依据。 相似文献
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对互助县朱尔沟小流域不同植被类型小区的径流泥沙观测分析,结果表明:保水保土效益以柠条林最好,其他由强到弱分别为六至九年生沙棘林地、二至五年生芨芨草地、天然草地,陡坡农耕地水土流失最为严重。 相似文献
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XU Hai-xia ZHANG Xia WANG Shao-ming YAN Ping DU Jin-zhou College of Life Sciences Shihezi University Shihezi 《(《农业科学与技术》)编辑部》2008,(1)
[Objective] Study on the genetic diversities of A.splendens from different areas of xinjiang.[Method] The genetic diversities among seven populations and within two populations were analyzed by RAPD.[Result] Genetic clustering results presented that the relationships among populations of A.splendens are directly related with geographic positions,and within population are related with habitats.[Conclusion] RAPD technique can be used to study genetic diversity of A.splendens. 相似文献
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《(《农业科学与技术》)编辑部》2008,(4)
[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level. 相似文献
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Genetic Diversity in Main Cultivars of Safflower in Xinjiang Uighur Autonomous Region Based on RAPD Analysis 总被引:3,自引:0,他引:3
YUE Qing-ni GE Juan WANG Lei ZHANG Xia WANG Shao-ming WANG Jian-ming College of Life Sciences Shihezi University Shihezi 《(《农业科学与技术》)编辑部》2008,(4)
[Objective] Study on the genetic diversity in main cultivars of safflower distributing in Xinjiang Uighur Autonomous Region by means of RAPD makers.[Method] Genomic DNAs of 29 safflower accessions from Xinjiang Uighur Autonomous Region were extracted for PCR amplification using 20 RAPD primers.[Result] Totally 156 bands were amplified,among which 144 bands were polymorphic(accounting for 92.31%),indicating that safflower is endowed with plentiful genetic diversity.Based on the DNA fingerprint,the 29 safflower accessions were grouped into four populations,the classification results may be not related with ecological regionality.[Conclusion] RAPD technique is an available tool to analyze the genetic diversity of safflower germplasm at molecular level. 相似文献
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Genetic Diversity in Wild Populations of Poacynum hendersonii from Inland Arid Regions of Northwest China 总被引:3,自引:0,他引:3
ZHAO Jin-feng ZHANG Wei-ming PENG Xue-mei TANG Ze-zi GU Gong-ping LU Chang-mei College of Life Sciences Nanjing Normal University Nanjing Nanjing Institute for Comprehensive Utilization of Wild Plants 《(《农业科学与技术》)编辑部》2008,(4)
[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population. 相似文献
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《(《农业科学与技术》)编辑部》2008,(4)
[Objective] Study on the genetic diversity in wild populations of Poacynum hendersonii.[Method] Random amplified polymorphic DNA(RAPD)technique was employed to analyze the genetic diversity in five wild populations of P.hendersonii sampled from Xinjiang,Gansu and Qinghai provinces.[Result] Totally 165 clear and repeatable bands were generated in RAPD reaction by using 20 primers screened from 80 primers,of which 110 were polymorphic,accounting for 66.67%.At species level,Nei's gene diversity index(H),Shannon's information index(I)and genetic differentiation coefficient(Gst)were 0.220 5,0.304 7 and 0.908 2,respectively.P.hendersonii germplasm resources share a high level of genetic diversity,and genetic differentiation mainly exists among the populations.Results from genetic distances and cluster analysis showed that relationships among P.hendersonii populations were to some extent related with their geographical and climatic characters.[Conclusion] This study suggests that the conservation of P.hendersonii should focus on the protection of many populations,particularly the Qinghai population. 相似文献
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[目的]研究大叶白麻野生居群的遗传多样性。[方法]采用RAPD技术对分布于新疆、甘肃和青海的5个大叶白麻野生居群的遗传多样性水平进行检测。[结果]经筛选的20条引物共扩增出165条清晰、重复性好的DNA条带,其中多态性条带为110条,多态位点百分率(PPB)为66.67%。居群间Nei′s多样性指数为0.2205,Shannon’s信息指数为0.3047,遗传分化系数(Gst)为0.9082。大叶白麻具有较高的遗传多样性水平,遗传分化主要发生在居群间。聚类分析显示居群间的相似度与分布区的地理和气候特征相关。[结论]在利用大叶白麻资源的同时,应根据各居群的品质和遗传特征,对这些居群尤其是青海居群加以保护。 相似文献
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新疆红花主要栽培品种遗传多样性的RAPD分析 总被引:2,自引:1,他引:1
[目的]利用RAPD技术分析新疆红花栽培品种遗传多样性。[方法]提取29份新疆红花栽培品种的基因组DNA并利用所筛选的20条RAPD引物进行PCR扩增。[结果]共扩增出156条带,其中144条带具有多态性,多态性条带占92.31%,说明新疆的红花具有较丰富的遗传多样性;根据引物扩增出的DNA指纹图谱,运用UPGMA分析法可将29份红花种质聚类划分为4个主要类群,该类群划分结果与红花的生态地域性可能不存相关性。[结论]该方法可用于在分子水平上分析红花品种的遗传多样性。 相似文献
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羽毛针禾种群遗传多样性分析 总被引:1,自引:0,他引:1
[目的]从分子水平对羽毛针禾居群内、居群间的遗传分化进行研究,揭示其遗传多样性水平。[方法]使用11条RAPD引物对生长于古尔班通古特沙漠的优良固沙植物羽毛针禾进行居群遗传变异分析。[结果]7个居群76个样品共检测到125个位点,总多态位点百分比为96.8%,居群内平均多态位点百分比为45.3%;羽毛针禾种群Shannon表型多样性指数(I)为0.5151,Nei’s基因多样度指数(h)为0.3471;居群间的基因分化系数为0.5284,即有52.84%的遗传多样性存在于居群间。[结论]羽毛针禾居群有较为丰富的遗传变异,且各居群间已有了明显分化。 相似文献
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[目的]对峨眉黄连及其近缘种进行遗传多样性分析。[方法]利用优化的RAPD-PCR反应体系及82条RAPD引物对11个不同生境的黄连属植物样本进行遗传多样性分析。[结果]11个黄连植物样本的多态率为89.23%,而对来源于4个生境的7个峨眉黄连样本,其多态率为38.80%。POPGENE结果显示,峨眉黄连种内遗传多样性水平不高,Nei’s基因多样性指数为0.125 0、Shannon信息指数为0.185 2、遗传分化指数Gst为0.368 0。[结论]该试验表明生境差异和基因流障碍可能是导致峨眉黄连生境间遗传差异的主要原因,遗传多样性与生长环境之间表现出了明显的相关性。 相似文献