3种生长调节物质对生菜不定芽直接再生的影响

应用“３１１－Ｂ”回归最优设计研究ＡｇＮＯ３，ＡＢＡ，ＡＳＡ对生菜离体培养不定芽直接再生的影响。结果表明，ＡｇＮＯ３是良好的乙烯抑制剂，与ＡＢＡ配合使用可明显抑制愈伤产生，大大促进不定芽的直接再生；而ＡＳＡ未曾抑制愈伤产生，不是乙烯抑制剂。     

芥菜型油菜再生体系的影响因素

对新疆芥菜型油菜４个单、双低吕系子叶柄再生频率的影响因素进行了研究,其中子叶柄日龄、６－ＢＡ、ＡｇＮＯ３、卡那霉素浓度及基因型等因素对不定芽的再生率有很大影响。试验结果表明：萌发４～５ｄ的幼苗子叶柄再生频率最高;６－ＢＡ、ＡｇＮＯ４对不定芽分化的促进效果在不同品系中有大差异,通常浓度为４～６ｍｇ／Ｌ。不同品系对卡那霉素的耐受力有很大不同,其中新９５－Ａ在Ｋａｎ１５ｍｇ／Ｌ时仍有４．１７％的绿苗率;     

几种化合物对土壤脲酶抑制作用动力学

茶多酚，硼砂、ＡｇＮＯ３均具有抑制脲酶活性的作用，其抑制效率随浓度增大而提高，茶多酚和硼砂对脲酶的抑制为非竞争性抑制，ＡｇＮＯ３则为反竞争性抑制。     

叶用莴苣高频率再生体系的建立

以４份叶用莴苣栽培品种为材料，比较了激素组合、ＡｇＮＯ３及其浓度和不同外植体切段地不定芽形成的影响，结果表明，在本实验条件下ＺＴ１ｍｇ／Ｌ和ＩＡＡ１ｍｇ／Ｌ组合的出芽外植体在分率最高，平均为２０．１４％；培养基中添加ＡｇＮＯ３可明显提高植株再生频率，增加每个外植体的丛生芽数目，浓度以５ｍｇ／Ｌ为最好，具芽外植体百分率平均为４４．９０％；真叶的培养效果好于子叶，真叶柄的培养效果优于真叶其它部位，以真     

实验室废液中银的回收研究

分别用ＮａＣｌ溶液、氨水、抗坏血酸溶液处理含银废液，将其中的银转化为单质银，其用稀ＨＮＯ３溶解后，即制成完全符合容量分析标准的ＡｇＮＯ３溶液。此溶液可以稀释成各种浓度使用；也可以蒸发浓缩至干，用水和乙醇混合溶剂重结晶，在１２０℃下烘干２ｈｒ．，即制成固体ＡｇＮＯ３，其纯度可以达到９９．８％以上，杂质含量与市售分析纯试剂相比较也非常接近。     

提高油菜芽苗分化频率研究

采用ＭＳ基本培养基, 加不同组合的激素对２个甘蓝型油菜和２个白菜型油菜的不同外植体进行培养,其中特别比较了ＡｇＮＯ３以及水解酪蛋白和谷氨酰胺对芽苗分化和促进作用,以期获得影响芽苗分化的必要因素。     

正交设计在石刁柏组织培养试验中的应用

运用正交试验设计对诱导石刁柏愈伤组织的生长素与激动素的比例，胚性愈伤组织的转化进行了研究。结果如下：（１）ＮＡＡ：ＢＡ为２：１；（２）ＢＡ的起始量为１ｍｇ·Ｌ＾－１；（３）ＡｇＮＯ３和３种氨基酸（Ａｓｎ＋Ｇｌｎ ａｎｄ Ｐｒｏ）对诱导胚性愈伤组织无显影响。     

实验室废弃中银的回收研究

分别用ＮａＣｌ溶液，氨水，抗坏血酸溶液处理含银废液，将其中的银转化为单质银，其用稀ＨＮＯ３溶解后，即制成完全符合容量分析标准的ＡｇＮＯ３溶液。此溶液可以稀释成各种浓度使用；也可以蒸发浓缩至干，用水和乙醇混合溶剂重结晶，在１２０℃下烘干２ｈｒ，即制成固体ＡｇＮＯ３，其纯度可以达到９９．８％以上，杂质含量与市售分析纯试剂相比较也非常接近。     

黄瓜雌性系诱导诱雄药剂及其应用效果初探

采用不同浓度的ＧＡ，ＡｇＮＯ３，ＡｇＳ２Ｏ３于不同时期诱导黄瓜雌性素，结果以Ａｇ２Ｓ２Ｏ３３００ｍｇ／ｌ于２片真叶期处理２次的诱导效果最强，且最安全，喷药时期的温度以１８－３０℃为佳，温度太低易导致幼苗猝倒，温度太高则植株上过渡型的无花节位增多，出现雄花节位数减少。     

花椰菜高频再生系统的研究

本文研究了ＢＡ、ＮＡＡ、ＡｇＮＯ３、子叶龄、培养密度以及Ｋｍ对花椰菜子叶再生和影响，建立了一个高频再生系统，使再生频率达１００％，其最佳各成分的配比是：ＭＳ＋ＢＡ４．５ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ＋ＡｇＮＯ３５．０￣１０．０ｍｇ／Ｌ。     

Web2.0,以个人为中心的互联网时代的到来

本文介绍了Web2．0的知识和背景，对Web2．0出现的基础以及典型技术和应用作了阐述，同时对其作出了前景展望。     

市级综合气象信息分析与管理系统的设计与实现

以抚顺地区3个国家基本站50余年气象资料为依托，采用Microsoft Excel工作表实现由气候和农业气候2部分构成的气象与农业综合信息处理系统的基本思路、方法、内容、用途和使用情况。为了实现气候分析及应用的科学化、客观化、系统化、规范化、程序化及连续化的业务目标，按业务流程设计与实现的，包括资料处理、气候趋势模拟、气候预测、气候鉴定、季节性信息化处理、农作物产量预报、气候生产潜力分析等一系列内容在内的系统建设。关键是解决了大量的计算问题，建立了区域性通用模式。     

Multi-variable grey model (MGM (1,n,q)) based on genetic algorithm and its application in urban water consumption

Urban water consumption has some characteristics of grey because it is influenced by economy, population, standard of living and so on. The multi-variable grey model （MGM（1,n））, as the expansion and complement of GM（1,1） model, reveals the relationship between restriction and stimulation among variables, and the genetic algorithm has the whole optimal and parallel characteristics. In this paper, the parameter q of MGM（1,n） model was optimized, and a multi-variable grey model （MGM（1 ,n,q）） was built by using the genetic algorithm. The model was validated by examining the urban water consumption from 1990 to 2003 in Dalian City. The result indicated that the multi-variable grey model （MGM（1,n,q）） based on genetic algorithm was better than MGM（1,n） model, and the MGM（1,n） model was better than MGM（1,1） model.     

Molecular detection of virulence genes in Campylobacter species isolated from livestock production systems in South Africa

Campylobacter species are a major cause of foodborne bacterial infections in both developed and developing countries worldwide. Campylobacter jejuni is responsible for the majority of infections. This study was conducted to identify virulenceassociated genes in Campylobacter species isolated from livestock production systems in South Africa. A total of 250 fecal samples consisting of cattle(n=50), chickens(n=50), goats(n=50), sheep(n=50) and pigs(n=50) were randomly collected from livestock in Eastern Cape and KwaZulu-Natal provinces of South Africa between April and October 2018. The samples were analyzed for the presence of virulence genes in Campylobacter species using molecular PCR-based methods. It was found that 77 and 23% of Campylobacter jejuni and Campylobacter coli respectively were isolated from all the livestock samples. There were positive significant(P0.05) correlations amongst all the virulence genes that were investigated. Chisquare and Fisher's exact tests were implemented to test for the effect of livestock species on the presence or absence of virulence genes. The study demonstrated that most of livestock species can potentially cause zoonotic infections and food poisoning due to the high prevalence of Campylobacter. The high prevalence of virulence genes highlights the significance of Campylobacter in livestock production systems in South Africa. This requires the implementation of one-health approaches to reduce the impact of foodborne and zoonotic diseases for the welfare of human and animal health.     

不同施肥水平对木薯氮磷钾养分积累、分配及其产量的影响

 【目的】通过比较不同施肥水平下木薯氮磷钾养分积累、分配和产量的差异，探讨粤北坡岗地优质高产木薯氮磷钾养分的最佳用量。【方法】以木薯品种南美119为材料，采用“3414”方案，在粤北翁源进行大田试验。试验共设14个处理。【结果】施肥处理的氮素主要分配到地上部，不施肥处理的氮素则主要分配到根部；不同施肥处理的磷素均主要分配到地上部；不施肥处理和不施钾处理的钾素主要分配到根部，不施氮处理的钾素在根、冠间分配较均衡。在木薯不同生长阶段，植株氮磷钾含量均呈下降趋势，但施氮、磷、钾化学肥料处理植株氮磷钾含量的下降速度小于不施氮、磷、钾化学肥料的处理。处理N2P2K2的氮、磷、钾含量和积累量在各生育期均为最大，不施肥处理（N0P0K0）的氮、磷、钾含量和积累量在各生育期均为最小（P＜0.05）。产量最高的是N2P3K2处理，为22 694.06 kg·hm-2，其次是N2P2K2处理，为21 417.87 kg·hm-2。【结论】木薯产量、氮磷钾养分积累及其在根冠间的分配，对施肥水平高度敏感, 适当比例的氮磷钾肥配合施用既可以显著增加木薯植株的氮磷钾含量和积累量，又可以提高产量。在粤北坡岗地木薯生产中，氮素最重要，在氮素得到满足的条件下，磷素较钾素更重要。在本试验条件下，氮磷钾养分最佳用量为358.80 kgN·hm-2、89.10 kg P2O5·hm-2和187.50 kg K2O·hm-2。     

红苕饲粮中添加铜和/或喹乙醇对猪生产性能及某些血液生化指标的影响

选体重约38公斤的长白(♂)×成华(♀)杂交猪20头,随机分为四组,饲予四种不同的饲粮,研究饲粮中添加铜和/或喹乙醇对猪生产性能和某些血液生化指标的影响。结果表明:基础组、加铜组、加喹乙醇组和两者同时加组全期日增重分别为674、761、796、678克,日采食量分别为2.50、2.65、2.74、2.40公斤,饲料利用率分别为3.73、3.49,3.44、3.54,添加铜和/或喹乙醇对试验第65天血清铜兰蛋白、尿素氨及屠宰率、背膘厚和眼肌面积无显著影响(p>0.05);添加铜使65天血清碱性碱酸酶活性极显著增高(p<0.01),添加喹乙醇有使之升高的趋势,两者同时添加时,碱性酸磷酶活性降低。     

Current progress on genetic interactions of rice with rice blast and sheath blight fungi

Analysis of genetic interactions between rice and its pathogenic fungi Magnaporthe oryzae and Rhizoctonia solani should lead to a better understanding of molecular mechanisms of host resistance, and the improvement of strategies to manage rice blast and sheath blight diseases. Currently, dozens of rice resistance (R) genes against specific races of the blast fungus have been described. Among them, ten were molecularly characterized and some were widely used for breeding for genetic resistance. The Pi-ta gene was one of the best characterized rice R genes. Following the elucidation of its molecular structure, interaction, distribution, and evolution, user friendly DNA markers were developed from portions of the cloned genes to facilitate the incorporations of the Pi-ta mediated resistance into improved rice varieties using marker assisted selection (MAS). However, rice blast is still a major threat for stable rice production because of race change mutations occurring in rice fields, which often overcome added resistance based on single R genes, and these virulent races of M. oryzae pose a continued challenge for blast control. For sheath blight, progress has been made on the exploration of novel sources of resistance from wild rice relatives and indica rice cultivars. A major quantitative trait locus (QTL), named qSB9-2, was recently verified in several mapping populations with different phenotyping methods, including greenhouse methods. The ability to identify qSB9-2 using greenhouse methods should accelerate the efforts on the qSB9-2 fine mapping and positional cloning.     

Virus Location and Lymphocyte Apoptosis in Lymph Nodes of Piglets Infected with PCV-2

The present study has been performed to understand the location of the virus, type of apoptotic cells, and their relation to lymph nodes of piglets infected with porcine circovirus type Ⅱ （PCV-2）. Nine 32-day-old conventional piglets free of infection with PCV-2 were used, and distributed into three groups： control group （n = 3）, piglets inoculated with PCV-2 alone （PCV-2, n = 3）, and PCV-2 inoculated and KLH immunostimulated group （PCV-2 ＋ KLH, n = 3）. Superficial inguinal lymph nodes from all piglets were collected for histological examination after 32 days postinoculation, and immunohistochemistry for PCV-2 detection. Location of apoptotic cells was detected with TdT-mediated dUTP nick end labeling （TUNEL） and cell cycle, and the apoptotic rates were measured by flow cytometry. The characteristic histopathological lesions of the piglets in PCV-2 and PCV-2 ＋ KLH were lymphocyte depletions in the cortex and paracortex of the lymph nodes, epithelioid-like macrophage infiltration, and intracytoplasmic inclusion bodies presented in epithelioid-like macrophages. PCV-2 was mainly found in epithelioid-like macrophages by immunohistochemistry. In the lymph nodes, lymphocytes presented higher apoptotic rates in the cortex by TUNEL, special B-cell areas, and similar apoptotic cells were found in this compartment in the control. The apoptotic rates of the lymph nodes were 0.41, 3.34, and 4.88% in the control, PCV-2, and PCV-2 ＋ KLH groups by flow cytometry, respectively. The apoptotic rates of lymph nodes for PCV-2 and PCV-2 ＋ KLH piglets were significantly higher than those for the control group （P〈0.05 and P〈0.01）. The proliferation index （PI） was 0.17_＋0.01, 0.12_＋0.01 and 0.12_＋0.04 in the control, PCV-2, and PCV-2 ＋ KLH group, the PI of the control group was higher than that of the other groups, but without the statistical difference. PCV-2 can induce lymphocyte depletion in lymph nodes of piglets by blocking cell proliferation and promoting apoptosis. This is one o     

锌对镉·汞复合污染下豌豆幼苗生长的影响

以豌豆为试验材料,采用水培方法研究了锌对镉、汞复合污染下的豌豆种子萌发与生长的影响。结果显示,锌浓度≤100.0 mg/L时促进豌豆种子的萌发,锌浓度为200.0和300.0 mg/L时抑制豌豆种子的萌发。当镉、汞的浓度为3.0 mg/L时,100.0 mg/L锌的解毒效应最明显,表现为能够促进镉、汞胁迫下豌豆种子萌发和幼苗生长,降低豌豆幼苗根尖细胞畸变率,提高豌豆幼苗的叶绿素含量及过氧化物酶活性;但当锌浓度≥200.0 mg/L时,与镉、汞协同迫害豌豆幼苗的生长。     

仙人掌超氧化物歧化酶提取与纯化研究

以仙人掌为试验材料,采用硫酸铵分级沉淀法得到超氧化物歧化酶（Superoxide Dismutase,SOD）粗酶液。然后用Sephadex（葡聚糖凝胶）G-75层析柱层析对粗酶液进行进一步纯化,最后用邻苯三酚法测定酶活力,并对酶活力主要影响因素（包括pH值、温度、抑制剂）进行了研究。结果显示仙人掌SOD酶比活力为751.56U.mg-1。pH值为7.5~8.5时酶活力最大,稳定性也好。温度维持在25~30℃时酶活力最高。由于过氧化氢和氰化钾均能使仙人掌SOD的酶活力显著下降,因而可以初步判断从仙人掌中提取到的SOD是Cu.Zn-SOD。