高油大豆新品种吉育87的选育及栽培要点

吉育87是吉林省农业科学院大豆研究中心选育的高油、高产大豆新品种,2006年通过吉林省农作物品种审定委员会审定,原品系代号公交DY2003-5。该品种具有高油、高产、抗病、广适等特点。生育期122~125 d,亚有限结荚习性,株高90 cm,尖叶、紫花,灰色绒毛,籽粒椭圆形,种皮黄色有光泽,种脐黄色。脂肪含量22.64%,蛋白质含量40.28%。2004-2005年,吉育87在吉林省中早熟组区域试验中平均产量3 219.6 kg/hm2,居第1位,比对照品种黑农38号增产9.8%。     

高油大豆新品种吉育90的选育及栽培要点

吉育90大豆新品种是吉林省农业科学院大豆研究中心在公主岭选育的高油、高产大豆新品种,2007年1月通过吉林省农作物品种审定委员会审定,原品系代号公交DY2003-1。该品种具有高油、高产、抗病、广适等特点。生育期130 d,亚有限结荚习性,株高115 cm,圆叶,紫花,灰色绒毛,籽粒椭圆形,种皮黄色有光泽,种脐黄色。脂肪含量22.28%,蛋白质含量38.07%。2005-2006年,吉育90在吉林省中晚熟组区域试验中平均产量3 056.3 kg/hm2,居第1位,比对照品种吉林30号增产10.0%。     

高油大豆新品种吉育84的选育

吉育84是吉林省农业科学院大豆研究中心选育的高油高产大豆新品种,2006年1月通过吉林省农作物品种审定委员会审定,原品系代号为公交DY2003-3。该品种具有高油、高产、抗病、广适等特点,生育天数122～125d,亚有限结荚习性,株高90cm,圆叶、紫花,灰色绒毛,籽粒椭圆形,种皮黄色有光泽,种脐黄色。脂肪含量23.22%,蛋白质含量40.16%。2004-2005年,在吉林省中早熟组区域试验中,吉育84平均产量2744.6kg/hm2,居第1位,比对照品种白农6号增产15.3%。     

吉林高油大豆新品种吉育100的选育及栽培要点

为了选育出适宜吉林省生态区种植的优质、高产大豆新品种,以具有目标性状的优良大豆亲本吉育47为母本、东2481为父本进行杂交选育,对其杂交后代材料采用系谱法进行繁殖鉴选,选育出了高油、高产、抗病、广适性好的大豆新品种吉育100。该品种在2006—2007年吉林省的中早熟西组区域试验中,平均产量2 248.5kg/hm2,居参试品种第1位,比白农6号(对照)增产14.3%,生育期127d,亚有限结荚习性,株高95cm,园叶、紫花,灰色绒毛,籽粒椭圆形,种皮黄色有光泽,种脐黄色,脂肪含量23.61%,蛋白质含量40.68%。2009年1月通过吉林省农作物品种审定委员会审定,并命名为吉育100(原品系代号公交DY2005-5)。     

油菜新品种高油9号特征特性及其高产栽培技术

高油9号油莱是山西省农业科学院高寒区作物研究所以蒙1008为母本。怀仁黄芥为父本进行有性杂交选育而成的高产、优质芥菜型春油菜新品种，2006年通过山西省农作物品种审定委员会审定。2003-2004年在山西省生产试验中，高油9号平均产量1452kg／hm^2．比对照晋油6号增产5．8％。高油9号合油量33．1％，油酸含量41．6％，亚油酸含量29．9％，芥酸含量1．1％，是一个低芥酸优质春油莱新品种。经过几年的栽培试验，已总结出高油9号油菜配套高产栽培技术。     

高异黄酮含量、高产大豆新品种吉育94选育及栽培技术

吉育94大豆新品种是吉林省农业科学院大豆研究中心育成,该品种异黄酮含量4.23‰,是一般品种的2～3倍,蛋白质含量38.34%,脂肪含量20.78%。抗大豆花叶病毒病和大豆胞囊线虫病,抗倒伏,为高产、高异黄酮含量品种。     

高油大豆新品种吉林48的选育报告

高油、早熟大豆新品种吉林 4 8是由吉林省农业科学院大豆研究所于 1992年经有性杂交选育而成 ,原品系编号为公交 92 0 9_8。突出特点为高油 (脂肪含量 2 2 2 5% )、熟期早、抗大豆灰斑病、中抗大豆花叶病 1～ 3号株系、丰产及稳产     

四川高EGCG茶树资源筛选研究

本试验选取四川茶区33个地方茶树资源作为供试材料,取其春梢1芽2叶蒸青固样,进行儿茶素总量、EGCG及主要生化成分含量等分析,以期发现EGCG含量高的茶树品种.结果表明,参试材料蒙山11号、N-2、N-3、N-4、N-5、N-6和花秋1号的EGCG含量≥9.0％,具有生产高EGCG茶的物质基础.     

利用国外大豆资源选育高油种质研究初报

研究以国外大豆种质资源(SPRIT87、HOBBIT)为核心亲本,与国内当地育成的品种(合丰42号) 为改良亲本,在杂交育种遗传改良的基础上,与辐射诱变育种技术结合,经过连续定向选择,利用品质分 析、抗病鉴定等方法,选育出合辐04-40、合辐04-46和合辐04-52等3个高油新种质,油分含量 23．95％-24．20％,产量2259．0-2779．5 kg/hm2,产量较对照品种增产11．6％-21．2％,抗灰斑病。报道高油种 质创新研究结果。     

高含油量双低油菜新品种苏油5号的选育

苏油5号是通过品种(系)间复合杂交的方法选育的高含油量双低甘蓝型油菜新品种,其芥酸含量0.55％,硫苷含量21.79 μmol/g,含油量46.33％.在上海市油菜区域试验中,平均产量为2 769.60 kg/hm2,产油量1 264.05 kg/hm2,比对照沪油15增7.72％.在上海市油菜生产试验中,平均产量为2 594.25 kg/hm2,平均产油量1 232.55 kg/hm2,比对照沪油15增27.68％.     

Web2.0,以个人为中心的互联网时代的到来

本文介绍了Web2．0的知识和背景，对Web2．0出现的基础以及典型技术和应用作了阐述，同时对其作出了前景展望。     

Virus Location and Lymphocyte Apoptosis in Lymph Nodes of Piglets Infected with PCV-2

The present study has been performed to understand the location of the virus, type of apoptotic cells, and their relation to lymph nodes of piglets infected with porcine circovirus type Ⅱ （PCV-2）. Nine 32-day-old conventional piglets free of infection with PCV-2 were used, and distributed into three groups： control group （n = 3）, piglets inoculated with PCV-2 alone （PCV-2, n = 3）, and PCV-2 inoculated and KLH immunostimulated group （PCV-2 ＋ KLH, n = 3）. Superficial inguinal lymph nodes from all piglets were collected for histological examination after 32 days postinoculation, and immunohistochemistry for PCV-2 detection. Location of apoptotic cells was detected with TdT-mediated dUTP nick end labeling （TUNEL） and cell cycle, and the apoptotic rates were measured by flow cytometry. The characteristic histopathological lesions of the piglets in PCV-2 and PCV-2 ＋ KLH were lymphocyte depletions in the cortex and paracortex of the lymph nodes, epithelioid-like macrophage infiltration, and intracytoplasmic inclusion bodies presented in epithelioid-like macrophages. PCV-2 was mainly found in epithelioid-like macrophages by immunohistochemistry. In the lymph nodes, lymphocytes presented higher apoptotic rates in the cortex by TUNEL, special B-cell areas, and similar apoptotic cells were found in this compartment in the control. The apoptotic rates of the lymph nodes were 0.41, 3.34, and 4.88% in the control, PCV-2, and PCV-2 ＋ KLH groups by flow cytometry, respectively. The apoptotic rates of lymph nodes for PCV-2 and PCV-2 ＋ KLH piglets were significantly higher than those for the control group （P〈0.05 and P〈0.01）. The proliferation index （PI） was 0.17_＋0.01, 0.12_＋0.01 and 0.12_＋0.04 in the control, PCV-2, and PCV-2 ＋ KLH group, the PI of the control group was higher than that of the other groups, but without the statistical difference. PCV-2 can induce lymphocyte depletion in lymph nodes of piglets by blocking cell proliferation and promoting apoptosis. This is one o     

市级综合气象信息分析与管理系统的设计与实现

以抚顺地区3个国家基本站50余年气象资料为依托，采用Microsoft Excel工作表实现由气候和农业气候2部分构成的气象与农业综合信息处理系统的基本思路、方法、内容、用途和使用情况。为了实现气候分析及应用的科学化、客观化、系统化、规范化、程序化及连续化的业务目标，按业务流程设计与实现的，包括资料处理、气候趋势模拟、气候预测、气候鉴定、季节性信息化处理、农作物产量预报、气候生产潜力分析等一系列内容在内的系统建设。关键是解决了大量的计算问题，建立了区域性通用模式。     

Multi-variable grey model (MGM (1,n,q)) based on genetic algorithm and its application in urban water consumption

Urban water consumption has some characteristics of grey because it is influenced by economy, population, standard of living and so on. The multi-variable grey model （MGM（1,n））, as the expansion and complement of GM（1,1） model, reveals the relationship between restriction and stimulation among variables, and the genetic algorithm has the whole optimal and parallel characteristics. In this paper, the parameter q of MGM（1,n） model was optimized, and a multi-variable grey model （MGM（1 ,n,q）） was built by using the genetic algorithm. The model was validated by examining the urban water consumption from 1990 to 2003 in Dalian City. The result indicated that the multi-variable grey model （MGM（1,n,q）） based on genetic algorithm was better than MGM（1,n） model, and the MGM（1,n） model was better than MGM（1,1） model.     

Molecular detection of virulence genes in Campylobacter species isolated from livestock production systems in South Africa

Campylobacter species are a major cause of foodborne bacterial infections in both developed and developing countries worldwide. Campylobacter jejuni is responsible for the majority of infections. This study was conducted to identify virulenceassociated genes in Campylobacter species isolated from livestock production systems in South Africa. A total of 250 fecal samples consisting of cattle(n=50), chickens(n=50), goats(n=50), sheep(n=50) and pigs(n=50) were randomly collected from livestock in Eastern Cape and KwaZulu-Natal provinces of South Africa between April and October 2018. The samples were analyzed for the presence of virulence genes in Campylobacter species using molecular PCR-based methods. It was found that 77 and 23% of Campylobacter jejuni and Campylobacter coli respectively were isolated from all the livestock samples. There were positive significant(P0.05) correlations amongst all the virulence genes that were investigated. Chisquare and Fisher's exact tests were implemented to test for the effect of livestock species on the presence or absence of virulence genes. The study demonstrated that most of livestock species can potentially cause zoonotic infections and food poisoning due to the high prevalence of Campylobacter. The high prevalence of virulence genes highlights the significance of Campylobacter in livestock production systems in South Africa. This requires the implementation of one-health approaches to reduce the impact of foodborne and zoonotic diseases for the welfare of human and animal health.     

不同施肥水平对木薯氮磷钾养分积累、分配及其产量的影响

 【目的】通过比较不同施肥水平下木薯氮磷钾养分积累、分配和产量的差异，探讨粤北坡岗地优质高产木薯氮磷钾养分的最佳用量。【方法】以木薯品种南美119为材料，采用“3414”方案，在粤北翁源进行大田试验。试验共设14个处理。【结果】施肥处理的氮素主要分配到地上部，不施肥处理的氮素则主要分配到根部；不同施肥处理的磷素均主要分配到地上部；不施肥处理和不施钾处理的钾素主要分配到根部，不施氮处理的钾素在根、冠间分配较均衡。在木薯不同生长阶段，植株氮磷钾含量均呈下降趋势，但施氮、磷、钾化学肥料处理植株氮磷钾含量的下降速度小于不施氮、磷、钾化学肥料的处理。处理N2P2K2的氮、磷、钾含量和积累量在各生育期均为最大，不施肥处理（N0P0K0）的氮、磷、钾含量和积累量在各生育期均为最小（P＜0.05）。产量最高的是N2P3K2处理，为22 694.06 kg·hm-2，其次是N2P2K2处理，为21 417.87 kg·hm-2。【结论】木薯产量、氮磷钾养分积累及其在根冠间的分配，对施肥水平高度敏感, 适当比例的氮磷钾肥配合施用既可以显著增加木薯植株的氮磷钾含量和积累量，又可以提高产量。在粤北坡岗地木薯生产中，氮素最重要，在氮素得到满足的条件下，磷素较钾素更重要。在本试验条件下，氮磷钾养分最佳用量为358.80 kgN·hm-2、89.10 kg P2O5·hm-2和187.50 kg K2O·hm-2。     

红苕饲粮中添加铜和/或喹乙醇对猪生产性能及某些血液生化指标的影响

选体重约38公斤的长白(♂)×成华(♀)杂交猪20头,随机分为四组,饲予四种不同的饲粮,研究饲粮中添加铜和/或喹乙醇对猪生产性能和某些血液生化指标的影响。结果表明:基础组、加铜组、加喹乙醇组和两者同时加组全期日增重分别为674、761、796、678克,日采食量分别为2.50、2.65、2.74、2.40公斤,饲料利用率分别为3.73、3.49,3.44、3.54,添加铜和/或喹乙醇对试验第65天血清铜兰蛋白、尿素氨及屠宰率、背膘厚和眼肌面积无显著影响(p>0.05);添加铜使65天血清碱性碱酸酶活性极显著增高(p<0.01),添加喹乙醇有使之升高的趋势,两者同时添加时,碱性酸磷酶活性降低。     

Current progress on genetic interactions of rice with rice blast and sheath blight fungi

Analysis of genetic interactions between rice and its pathogenic fungi Magnaporthe oryzae and Rhizoctonia solani should lead to a better understanding of molecular mechanisms of host resistance, and the improvement of strategies to manage rice blast and sheath blight diseases. Currently, dozens of rice resistance (R) genes against specific races of the blast fungus have been described. Among them, ten were molecularly characterized and some were widely used for breeding for genetic resistance. The Pi-ta gene was one of the best characterized rice R genes. Following the elucidation of its molecular structure, interaction, distribution, and evolution, user friendly DNA markers were developed from portions of the cloned genes to facilitate the incorporations of the Pi-ta mediated resistance into improved rice varieties using marker assisted selection (MAS). However, rice blast is still a major threat for stable rice production because of race change mutations occurring in rice fields, which often overcome added resistance based on single R genes, and these virulent races of M. oryzae pose a continued challenge for blast control. For sheath blight, progress has been made on the exploration of novel sources of resistance from wild rice relatives and indica rice cultivars. A major quantitative trait locus (QTL), named qSB9-2, was recently verified in several mapping populations with different phenotyping methods, including greenhouse methods. The ability to identify qSB9-2 using greenhouse methods should accelerate the efforts on the qSB9-2 fine mapping and positional cloning.     

仙人掌超氧化物歧化酶提取与纯化研究

以仙人掌为试验材料,采用硫酸铵分级沉淀法得到超氧化物歧化酶（Superoxide Dismutase,SOD）粗酶液。然后用Sephadex（葡聚糖凝胶）G-75层析柱层析对粗酶液进行进一步纯化,最后用邻苯三酚法测定酶活力,并对酶活力主要影响因素（包括pH值、温度、抑制剂）进行了研究。结果显示仙人掌SOD酶比活力为751.56U.mg-1。pH值为7.5~8.5时酶活力最大,稳定性也好。温度维持在25~30℃时酶活力最高。由于过氧化氢和氰化钾均能使仙人掌SOD的酶活力显著下降,因而可以初步判断从仙人掌中提取到的SOD是Cu.Zn-SOD。     

锌对镉·汞复合污染下豌豆幼苗生长的影响

以豌豆为试验材料,采用水培方法研究了锌对镉、汞复合污染下的豌豆种子萌发与生长的影响。结果显示,锌浓度≤100.0 mg/L时促进豌豆种子的萌发,锌浓度为200.0和300.0 mg/L时抑制豌豆种子的萌发。当镉、汞的浓度为3.0 mg/L时,100.0 mg/L锌的解毒效应最明显,表现为能够促进镉、汞胁迫下豌豆种子萌发和幼苗生长,降低豌豆幼苗根尖细胞畸变率,提高豌豆幼苗的叶绿素含量及过氧化物酶活性;但当锌浓度≥200.0 mg/L时,与镉、汞协同迫害豌豆幼苗的生长。