Molecular mapping of a stripe rust resistance gene in wheat cultivar Wuhan 2

Stripe rust (or yellow rust), caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat worldwide. Growing resistant cultivars is the best approach to control the disease. To identify and map genes for stripe rust resistance in wheat cultivar ‘Wuhan 2', an F₂ population was developed from a cross between the cultivar and susceptible cultivar Mingxian 169. The parents, 179 F₂ plants and their derived F_2:3 lines were evaluated for responses to Chinese races CYR30 and CYR31 of the pathogen in a greenhouse. A recessive gene for resistance was identified. DNA bulked segregant analysis was applied and resistance gene analog polymorphism (RGAP) and simple sequence repeat (SSR) techniques were used to identify molecular markers linked to the resistance gene. A genetic map consisting of five RGAP and six SSR markers was constructed. The recessive gene, designated Yrwh2, was located on the short arm of chromosome 3B and flanked by SSR markers Xwmc540 and Xgwm566 at 5.9 and 10.0 cM, respectively. The chromosomal location of the resistance gene and its close marker suggest that the locus is different from previously reported stripe rust resistance genes Yr30, QYr.ucw-3BS, Yrns-B1, YrRub and QYrex.wgp-3BL previously mapped to chromosome 3B. Yrwh2 and its closely linked markers are potentially useful for developing stripe rust resistance wheat cultivars if used in combination with other genes.     

小麦品种贵农21抗条锈病基因的SSR标记

对贵农21携带的条锈病 (Puccinia striiformis Westend f. sp. tritic) 抗性基因进行鉴定和遗传分析,明确了贵农21携带1个抗条锈病显性单基因,暂命名为YrGn21。采用F₂代抗病分离群体和集群分离分析法(BSA),建立了与YrGn21连锁的11个微卫星标记Xcau14、Xwmc49、Xgwm403、Xgdm62、Xwmc272、Xgwm459、Xbarc240、Xbarc187、Xgdm28、Xgwm11和Xgwm413,并将YrGn21定位于小麦1BS的近着丝粒区域,与位于1BS染色体上的Yr26基因具有等位性关系,为贵农21抗条锈病基因在育种中的利用,进行标记辅助选择和基因累加提供了便利。     

小麦品种小偃9323抗条锈基因的遗传分析和分子作图

小偃9323是小偃6号的同源材料,具有早熟、抗逆性强、适应性广、抗条锈性强等许多优良的生物学特性。为明确其抗条锈性及遗传规律,利用当前流行的中国条锈菌小种CYR32对抗病品种小偃9323与感病品种铭贤169及其杂交后代F₁、F₂、F₃和BC₁代进行苗期抗条锈性遗传分析,并对其抗条锈基因进行SSR分子标记。结果表明,小偃9323对CYR32小种具有良好的抗性,由1对隐性基因所控制。利用F₂代分离群体,筛选到6个与抗病基因连锁的SSR标记,分别是Xwmc807、Xbarc3、Xwmc684、Xwmc201、Xwmc553和Xwmc179;该抗病基因位于小麦6AL染色体上,其最近的标记为Xwmc201和Xwmc553,遗传距离分别是2.6 cM和3.7 cM。分析表明,该基因不同于已知抗条锈基因,暂被命名为YrXY9323。用YrXY9323两侧遗传距离最近的标记Xwmc201和Xwmc553对42个黄淮麦区主栽小麦品种进行分子检测,结果表明有19%的品种具有与YrXY9323相同的标记位点。本结果对YrXY9323在小麦抗条锈病育种中的应用提供了理论依据。     

小麦骨干亲本繁6条锈病成株抗性特异位点及其在衍生品种中的遗传解析

基于已获得的控制小麦条锈病成株抗性“一致性”QTL区段80个SSR标记,结合小麦骨干亲本繁6及其衍生的39个后代小麦品种进行田间条锈病成株期抗性表型鉴定,揭示了骨干亲本繁6遗传物质及其成株抗性在其衍生品种的遗传规律。结果表明,骨干亲本繁6在条锈病条中31、32和33混合生理小种诱导下表现成株抗性,7个衍生后代品种表现全生育期抗性;用控制小麦条锈病成株抗性QTL区段的80个SSR标记对繁6及其后代衍生品种的其他亲本进行分子扫描,共发现9个来自繁6基因组的特异SSR标记,即Xwmc631、 Xgwm359、 Xwmc407、 Xgwm501、 Xgwm148、 Xgwm539、 Xgwm533、 Xgwm299和Xgwm639,其中,Xwmc631、 Xgwm359、 Xgwm501、 Xgwm299和Xgwm639在繁6衍生后代的4个子代中表现较高的遗传贡献率。以SSR标记与小麦条锈病成株抗性的关联分析发现6个SSR标记与小麦条锈病成株抗性显著相关,其中来自繁6的特异SSR等位变异Xgwm539-2D和Xgwm299-3B与严重度、反应型、普遍率、病情指数及病程曲线下面积(AUDPC)均具显著相关性,表明繁6的成株抗性及其控制遗传位点在其衍生后代品种选育过程中得到了很好的定向选择,并在西南麦区小麦条锈病抗性育种中发挥了重要作用。     

源于叙利亚小麦ICA31抗条锈病基因分析及分子标记研究

遗传分析表明,小麦材料ICA31携带一个显性抗条锈病基因,对流行的优势条锈菌小种条中30,31,32免疫;据等位性测定,ICA31抗条锈基因与已知抗锈基因Yr5、Yr10、Yr15不等位;从抗源的系谱分析,该基因来源于叙利亚普通小麦品系叙18;利用微卫星标记和分组分析(BSA)法,筛选到与该抗条锈病基因（Yr-Syria）紧密连锁的SSR标记WMS11-193;对F2分离群体142个单株分析结果表明,该抗条锈病基因（Yr-Syria）与WMS11-193间遗传距离为2.1cM;将Yr-Syria定位于小麦1BS上;为该基因进行抗条锈小麦分子辅助育种打下基础。     

78份四川小麦育成品种(系)条锈病抗性鉴定与抗条锈病基因分子检测

四川省是小麦条锈菌新小种产生的重要地区之一,了解2016年以来四川小麦育成品种(系)对当前流行的条锈菌生理小种和致病类型的抗性水平以及明确其抗条锈病基因的分布状况,可为四川育种防控小麦抗条锈病和品种布局提供理论依据。本研究选择2个小种CYR32和CYR34对78份四川小麦育成品种(系)进行苗期鉴定,利用当前小麦条锈菌优势小种CYR32、CYR33、CYR34,以及贵22-14、贵农致病类群等混合菌进行成株期人工接种鉴定,并利用19个抗条锈病QTL和基因QYr.nwafu-4BL、Yr5、Yr10、Yr15、Yr17、Yr18、Yr26、Yr28、Yr29、Yr30、Yr36、Yr39、Yr41、Yr48、Yr65、Yr67、Yr78、Yr80和Yr81的分子标记对供试材料进行抗条锈病基因检测。结果表明,在78份供试材料的苗期鉴定中,对CYR32表现出抗性的有60份,占76.92%;对CYR34表现出抗性的有40份,占51.28%;同时对CYR32和CYR34表现抗性的有36份,占46.15%。78份小麦品种(系)在成株期均表现抗条锈病,其中绵麦835、蜀麦1743、蜀麦1829和蜀麦1868表现为免疫。苗期和成株期抗病性鉴定结果表明,成株期抗性材料有42份,占53.85%;全生育期抗性材料有36份,占46.15%。分子检测结果表明,可能携带QYr.nwafu-4BL、Yr15、Yr17、Yr18、Yr26、Yr28、Yr29、Yr30、Yr39、Yr41、Yr65、Yr67、Yr78、Yr80和Yr81的材料分别有5、5、45、2、30、5、30、39、3、2、22、8、23、6和24份。同时携带2~6个抗条锈病基因的聚合材料分别有24、22、11、14和3份,占94.87%。所有供试品种(系)均未检测到Yr5、Yr10、Yr36和Yr48,仅西科麦18未检测到上述19个抗条锈病基因,可能携带其他已知或新的条锈病抗性基因。本研究鉴定了78份四川小麦育成品种(系)对条锈病抗性水平整体较好,明确了其携带的抗条锈病基因,为利用其培育持久抗性小麦品种提供了科学依据。     

Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers

Yellow rust (stripe rust), caused by Puccinia striiformis Westend f. sp. tritici, is one of the most devastating diseases of wheat throughout the world. Wheat-Haynaldia villosa 6AL.6VS translocation lines R43, R55, R64 and R77, derived from the cross of three species, carry resistance to both yellow rust and powdery mildew. An F₂ population was established by crossing R55 with the susceptible cultivar Yumai 18. The yellow rust resistance in R55 was controlled by a single dominant gene, which segregated independently of the powdery mildew resistance gene Pm21 located in the chromosome 6VS segment, indicating that the yellow rust resistance gene and Pm21 are unlikely to be carried by the same alien segment. This yellow rust resistance gene was considered to beYr26, originally thought to be also located in chromosome arm 6VS. Bulked Segregation Analysis and microsatellite primer screens of the population F₂ of Yumai 18 × R55 identified three chromosome 1B microsatellite locus markers, Xgwm11, Xgwm18 and Xgwm413, closely linked to Yr26. Yr26 was placed 1.9 cM distal of Xgwm11/Xgwml8, which in turn were 3.2 cM from Xgwm413. The respective LOD values were 21 and 36.5. Therefore, Yr26 was located in the short arm of chromosome 1B. The origin and distribution of Yr26 was investigated by pedigree, inheritance of resistance and molecular marker analysis. The results indicated that Yr26 came from Triticum turgidum L. Three other 6AL.6VS translocation lines, R43, R64 and R77, also carried Yr26. These PCR-based microsatellite markers were shown to be very effective for the detection of the Yr26 gene in segregating populations and therefore can be applied in wheat breeding. This revised version was published online in July 2006 with corrections to the Cover Date.     

小麦品系M8003-5抗条锈病基因遗传分析和分子作图

为了利用小麦抗条锈病品系M8003-5中的抗病基因,用当前7个流行的条锈菌生理小种对小麦品系M8003-5的抗条锈性进行了鉴定,发现该品种对当前的各优势小种均有良好抗性。在温室内以病菌小种Su11-4对M8003-5在进行苗期抗条锈性鉴定和遗传分析,初步确定M8003-5对Su11-4的抗性由1对显性基因控制,位于7DS上的SSR标记Xbarc5、Xwmc463、Xwmc405、Xbarc126、Xgwm295、Xgwm44、Xwmc702、Xwmc438、Xwmc121、Xgwm111和Xbarc121与该基因连锁,最近的为Xwmc702和Xwmc438,遗传距离分别为3.5 cM和4.3 cM。分子标记及其相关分析表明,此基因可能来自黑麦,与已定位于7D染色体上的抗病基因不同,暂命名为YrM8003。利用与其紧密连锁的标记Xwmc702和Xwmc438测黄淮麦区43个主栽品种,结果显示,有20%的品种具有与YrM8003基因相同的标记位点。这一结果有助于YrM8003在抗条锈病育种的应用。     

硬粒小麦-粗山羊草人工合成小麦CI108抗条锈病新基因的鉴定、基因推导与分子标记定位

利用我国流行的小麦条锈菌生理小种CY28、CY29、CY30、CY31、CY32和水源11致病型4对102份硬粒小麦-粗山羊草人工合成小麦材料进行抗病鉴定,其中CI108（组合为GAN/Aegilops squarrosa 201）对上述6个流行生理小种均表现免疫。利用CY31对杂交组合CI108/铭贤169正交、反交的F₁材料以及F₂代群体进行抗病鉴定,结果表明其抗性受细胞核显性单基因控制。基因推导表明,CI108对30个条锈菌生理小种均表现抗性,其抗谱与23份已知抗条锈病基因品种（系）不同,与K733（含有Yr24）和洛夫林13（含Yr9+未知基因）相似,但CI108与洛夫林13、K733对多个条锈菌生理小种的抗性程度不同,洛夫林13、K733与CI108系谱不同,且缺乏CI108特异的SSR标记Xgwm456的抗病特异带。所以,CI108中抗条锈基因应该是不同于其他基因的抗条锈病新基因,暂命名为YrC108。进一步利用CI108/铭贤169的F₂群体、抗感分离分析池（BSA）筛选YrC108的SSR分子标记,找到了3个紧密连锁的标记,其中Xgwm456和Wmc419位于YrC108的一侧,与YrC108间遗传距离分别为0.6 cM和1.8 cM,Wmc413位于YrC108的另一侧,遗传距离为0.6 cM。本研究为小麦抗条锈病育种提供了高抗、广谱的新抗源和进行高效检测的分子标记。     

Inheritance and gene tagging of male fertility restoration of cytoplasmic-nuclear male-sterile line NJCMS1A in soybean

The F₁, F₂ and F_2:3 of the NJCMS1A × 'Zhongdou 5' cross were used to analyse the inheritance of the male fertility restoration of the cytoplasmic-nuclear male-sterile line NJCMS1A in soybean. The results of genetic analysis showed two pairs of dominant genes conferring the male fertility restoration of NJCMS1A, which further confirmed previous results. The F₂ population from the NJCMS1A × 'Zhongdou 5' cross was used for tagging the restorer genes for NJCMS1A with 664 pairs of simple sequence repeat primers selected randomly from the genetic linkage map of soybean published by Cregan et al. (1999) . Satt626 on linkage group M and Satt300 on linkage group A1 of the integrated linkage map by Song et al. (2004) were found to link to the two restorer genes of NJCMS1A. The maximum-likelihood estimates of the genetic distance between the two markers, Satt626 and Satt300, and the two restorer genes of 'Zhongdou 5' were 9.75 and 11.18 cM, respectively.     

Development of co-dominant amplified polymorphic sequence markers for resistance of sunflower to downy mildew race 730

The inheritance of the reaction of sunflower to downy mildew was investigated using resistant and susceptible near isogenic lines (NILs) and their F₃ families. Resistance to race 730 was evaluated using the whole seedling inoculation technique. Seventy-three F₃ families were inoculated, among which 54 families were resistant and 19 susceptible, fitting a 3 : 1 segregation ratio. F₃ families were also studied using several PCR markers. Ten markers at the Pl6 locus, specific for the resistant line, also segregated in F₃ families with a 3 : 1 ratio. The same segregation ratio occurred for microsatellite haplotypes that resembled the resistant parent, and were amplified with ORS 166 and ORS 1043. The only common fragment that was observed between resistant and susceptible parental lines was one of the TIR-NBS-LRR resistance gene analogue markers, having a restriction site. Two co-dominant cleaved amplified polymorphic sequence (CAPS) markers were obtained. The mapping data indicate that several dominant markers and two CAPS markers, developed here, completely co-segregate with the Pl6 gene conferring resistance to race 730. CAPS markers will facilitate efficient marker-assisted selection for sunflower resistance to downy mildew race 730.     

Development and agronomic performance of common bean lines simultaneously resistant to anthracnose, angular leaf spot and rust

The common bean is affected by several pathogens that can cause severe yield losses. Here we report the introgression of resistance genes to anthracnose, angular leaf spot and rust in the 'carioca-type' bean cultivar 'Rudá'. Initially, four backcross (BC) lines were obtained using 'TO', 'AB 136', 'Ouro Negro' and 'AND 277' as donor parents. Molecular fingerprinting was used to select the lines genetically closer to the recurrent parent. The relative genetic distances between 'Rudá' and the BC lines varied between 0.0% and 1.99%. The BC lines were intercrossed and molecular markers linked to the resistance genes were used to identify the plants containing the genes of interest. These plants were selfed to obtain the F₂, F₃ and F₄ plants which were selected based on the presence of the molecular markers mentioned and resistance was confirmed in the F₄ generation by inoculation. Four F_4:7 pyramid lines with all the resistance genes showed resistance spectra equivalent to those of their respective donor parents. Yield tests showed that these lines are as productive as the best 'carioca-type' cultivars.     

Identification and molecular mapping of a leaf rust resistance gene in spelt wheat landrace Altgold

Leaf rust, caused by Puccinia triticina, is an important disease for wheat production, both in China and worldwide. In laboratory studies spelt wheat (Triticum aestivum ssp. spelta) landrace Altgold was resistant to P. triticina races THT and PHT and genetic analysis indicated that it possessed a dominant leaf rust resistance gene, temporarily designated LrAlt. F₆ recombinant inbred lines (RILs) derived from a cross with the susceptible common wheat cultivar Nongda 3338 were used to map LrAlt with SSR markers. The resistance gene was distal to SSR loci Xbarc212, Xwmc382, Xgwm636, and Xwmc407 on the short arm of chromosome 2A. The closest markers Xbarc212 and Xwmc382 which co-segregated were 1.8 cM away from LrAlt. The relationships of LrAlt and other wheat leaf rust resistance genes located on the short arm of chromosome 2A were discussed, suggesting that LrAlt might be a new leaf rust resistance gene.     

QTL mapping of maize (Zea mays) stay-green traits and their relationship to yield

A maize genetic linkage map derived from 115 simple sequence repeat (SSR) markers was constructed from an F₂ population. The F₂ was generated from a cross between a stay-green inbred line (Q319) and a normal inbred line (Mo17). The map resolved 10 linkage groups and spanned 1431.0 cM in length with an average genetic distance of 12.44 cM between two neighbouring loci. A total of 14 quantitative trait loci (QTL) were detected for stay-green traits at different postflowering time intervals and identified by composite interval mapping. The respective QTL contribution to phenotypic variance ranged from 5.40% to 11.49%, with trait synergistic action from Q319. Moreover, maize stay-green traits were closely correlated to grain yield. Additional QTL analyses indicated that multiple intervals of stay-green QTL overlapped with yield QTL.     

Development of STS markers and QTL validation for common bacterial blight resistance in common bean

Common bacterial blight (CBB) of common bean ( Phaseolus vulgaris L.), is one of the major diseases that decrease yield and quality. A major quantitative trait locus (QTL) for CBB resistance from line XAN 159 was transferred into two bean lines, HR45 and HR67. Previous studies identified that two markers are linked to this QTL but the chromosome location was not consistent. To identify more tightly linked markers and to verify the chromosome location, 65 additional markers were mapped using 81 recombinant inbred lines (RILs) derived from a cross HR67 × OAC95-4. The QTL was mapped to a 13 cM region on chromosome 1 and defined by eight molecular markers that explained 25–52% of the phenotypic variation. Six tightly linked amplified fragment length polymorphism markers (0.6–9.7 cM from the QTL peak) were converted into seven sequence tagged site markers, three of which were mapped to this QTL. Five tightly linked markers were used to screen 907 F₂ plants derived from a cross HR45 × 'OAC Rex' and four of them were linked to each other within 4.2 cM. These markers may be useful in marker-assisted selection and map-based cloning of this major QTL.     

SSR and STS markers for wheat stripe rust resistance gene <Emphasis Type="Italic">Yr26</Emphasis>

Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating wheat diseases worldwide. Triticum aestivum-Haynaldia villosa 6VS/6AL translocation lines carrying the Yr26 gene on chromosome 1B, are resistant to most races of Pst used in virulence tests. In order to better utilize Yr26 for wheat improvement, we attempted to screen SSR and EST-based STS markers closely linked with Yr26. A total of 500 F₂ plants and the F_2:3 progenies derived from a cross between 92R137 and susceptible cultivar Yangmai 5 were inoculated with race CYR32. The analysis confirmed that stripe rust resistance was controlled by a single dominant gene, Yr26. Among 35 pairs of genomic SSR markers and 81 pairs of STS markers derived from EST sequences located on chromosome 1B, Yr26 was flanked by 5 SSR and 7 STS markers. The markers were mapped in deletion bins using CS aneuploid and deletion lines. The closest flanking marker loci, Xwe173 and Xbarc181, mapped in 1BL and the genetic distances from Yr26 were 1.4 cM and 6.7 cM, respectively. Some of these markers were previously reported on 1BS. Eight common wheat cultivars and lines developed from the T. aestivum-H. villosa 6VS/6AL translocation lines by different research groups were tested for presence of the markers. Five lines with Yr26 carried the flanking markers whereas three lines without Yr26 did not. The results indicated that the flanking markers should be useful in marker-assisted selection for incorporating Yr26 into wheat cultivars.     

Molecular mapping of a fertility restorer gene for cytoplasmic male sterility in soybean

In this study, we report the mapping of the Rf locus in soybean by microsatellite simple sequence repeat (SSR) genetic markers. A cross was made between cytoplasmic male sterility (CMS) line JLCMS82A and restorer line JIHUI 1 based on the DNA polymorphisms revealed by 109 SSR markers. A F₂ population derived from a single F₁ plant containing 103 individuals was used for mapping the Rf locus. The Rf gene of JIHUI 1 gametophytically restores male fertility to JLCMS82A. Fertile and semi-fertile DNA bulks and parental DNAs were screened with 219 SSR markers, and Satt215 which was previously mapped to soybean LG J, was found linked to the Rf gene. Five additional polymorphic SSR markers from LG J were used for analysis and a regional linkage map around the Rf locus was established. SSR markers, Sctt011 and Satt547, flanked the Rf locus at 3.6 cM and 5.4 cM, respectively. The availability of these SSR markers will facilitate the selection of restorer lines in hybrid soybean breeding.     

Molecular mapping of a new red mutant gene (Rs) in upland cotton

In recent years, there has been slow progress in improving cotton yield. It is known that the F₁ generation from the cross of the new red mutant and the normal green leaf plant has high photosynthetic efficiency. Therefore, cloning the new red mutant gene and further introducing it into other crops through transgenic techniques is a promising approach for achieving high photosynthetic efficiency through breeding. To map this new mutant gene, tentatively named R _s , the authors constructed an F₂ generation containing 1214 individual plants from mutant EH083 ( Gossypium hirsutum ) and Hai 7124 ( Gossypium barbadense ). Fifty-five pairs of simple sequence repeats and sequence-related amplified polymorphism (SRAP) primers on chromosome 7 were selected to screen the two parents. Finally, the R _s gene was mapped at the 0.3 cM interval flanked by markers NAU3735 and NAU1048.     

Identification of AFLP and RAPD markers linked to anthracnose resistance in grapes and their conversion to SCAR markers

Resistance to anthracnose or black spot ( Elsinoe ampelina ), a serious fungal pathogen in viticulture and table grape production, was investigated on 25 grape cultivars. Bioassays performed with culture filtrates produced by the pathogen revealed 14 resistant genotypes. In most plants resistance originated from Vitis labrucsa but also genotypes with V. rupestris and V. riparia  ×  V. rupestris background showed resistance. Genetic analysis was conducted in F₁, S₁ and BC₁ plants developed from various cultivars. In total, 326 F₁ plants were evaluated, 172 genotypes proofed to be resistant, whereas 154 were susceptible to anthracnose. A Mendelian segregation ratio of 1 : 1 (χ² = 0.30–0.65) indicating that anthracnose resistance is controlled by a single dominant gene. To facilitate the use of marker-assisted selection in grape-breeding PCR-based markers were developed by random amplified polymorphic DNA and amplified fragment length polymorphism in bulk segregant analysis. Finally, OPB 15₁₂₄₇ was developed as a sequence characterized amplified region marker being diagnostic for the locus of resistance to anthracnose in all resistant genotypes tested. Within the 25 grape cultivars OPB 15₁₂₄₇ is diagnostic in the genetic background of both V. labrucsa and V. rupestris and V. riparia  ×  V. rupestris .     

Cytogenetic analysis of F1, F2 and BC1 plants from intergeneric sexual hybridization between Sinapis alba and Brassica oleracea by genomic in situ hybridization

By intergeneric sexual hybridization between Sinapis alba and Brassica oleracea , F₁, F₂ and BC₁ progeny plants were produced. S. alba plants (genome SS, 2n = 24) were pollinated with B. oleracea (genome CC, 2n = 18), and the fertile F₁ plants were pollinated with B. oleracea to obtain BC₁ plants. GISH analysis showed that 10 out of 12 F₁ plants had 12 S. alba chromosomes (one full S chromosome set) and nine B. oleracea chromosomes (one C chromosome sets), representing the expected hybrids. However, two F₁ plants had 12 S chromosomes and 18 C chromosomes (two C chromosome sets), indicating unexpected hybrids. A maximum of three trivalents between C and S chromosomes were identified at metaphase I of semi-fertile F₁ pollen mother cells (PMCs), which indicates homology and chromosome pairing between these two genomes. The C genome had obviously been doubled in two F₂ plants from selfed semi-fertile F₁ plants. BC₁ plants consisted of 18 C chromosomes and different numbers of one, five and six additional S chromosomes, respectively. Monosomic alien addition lines developed in the present study can be used for B. oleracea breeding and Sinapis alba gene mapping.