An effective field screening method for flood tolerance in soybean

Flooding is an abiotic stress that causes considerable reductions in crop growth and yield worldwide. Soybean (Glycine max [L.] Merr.) cultivars are generally sensitive to flooding stress. The objective of this study was to develop an effective flooding tolerance screening method in the field. A total of 40 soybean genotypes were evaluated for flooding tolerance at V5 and R1 growth stages. At each stage, genotypes were exposed to different durations of flooding stress (3, 6, 9, 12 and 15 days). Plant foliar damage score (FDS) and plant survival rate (PSR) were used as the indicators of flooding tolerance. Soybeans were more sensitive to flooding at R1 growth stage than V5 growth stage. Length of flooding duration accounted for the variance of FDS and PSR. Soybean genotypes exposed to a 3‐day flooding in either V5 or R1 growth stage, did not show obvious foliar damage, while genotypes exposed to a 12‐ or 15‐day flooding showed significant foliar damage and plant death. The optimum flooding duration to screen for flooding tolerance in the field was determined to be 9 and 6 days for V5 and R1 growth stages, respectively, as distinguishable responses to flooding allowed genotypes to be classified as either being flooding tolerant or flooding sensitive. High correlation between FDS and PSR (.99, p < .0001) was observed. Similarly, FDS and PSR were highly correlated with grain yield (.95 and .95, p < .0001). The field screening method for flooding tolerance developed in our study will be favourable for selection of soybean flooding‐tolerant germplasm.     

Field evaluation of mixed-seedlings with rice to alleviate flood stress for semi-arid cereals

Flash floods, erratically striking semi-arid regions, often cause field flooding and soil anoxia, resulting in crop losses on food staples, typically pearl millet (Pennisetum glaucum L.) and sorghum (Sorghum bicolor (L.) Moench). Recent glasshouse studies have indicated that rice (Oryza spp.) can enhance flood stress tolerance of co-growing dryland cereals by modifying their rhizosphere microenvironments via the oxygen released from its roots into the aqueous rhizosphere. We tested whether this phenomenon would be expressed under field flood conditions. The effects of mix-planting of pearl millet and sorghum with rice on their survival, growth and grain yields were evaluated under controlled field flooding in semi-arid Namibia during 2014/2015–2015/2016. Single-stand and mixed plant treatments were subjected to 11–22 day flood stress at the vegetative growth stage. Mixed planting increased plant survival rates in both pearl millet and sorghum. Grain yields of pearl millet and sorghum were reduced by flooding, in both the single-stand and mixed plant treatments, relative to the non-flooded upland yields, but the reduction was lower in the mixed plant treatments. In contrast, flooding increased rice yields. Both pearl millet–rice and sorghum–rice mixtures demonstrated higher land equivalent ratios, indicating a mixed planting advantage under flood conditions. These results indicate that mix-planting pearl millet and sorghum with rice could alleviate flood stress on dryland cereals. The results also suggest that with this cropping technique, rice could compensate for the dryland cereal yield losses due to field flooding.     

Different Physiological Reactions at Vegetative and Generative Stage of Development of Field Bean Plants Exposed to Flooding and Undergoing Recovery

Plants at various stages of growth are differentiated in their susceptibility to environmental stresses and possibility of regeneration during recovery period after cessation of stress. The role of antioxidative enzymes in the stabilisation of the physiological state of field bean plants was studied. Catalase, peroxidises activity, and hydrogen peroxide accumulation were determined after 1 and 7 days of flooding and after 1 and 7 days of recovery from flooding. The influence of flooding on plants growth was analysed on the basis of leaf area index (LAI), relative growth rate (RGR) and net assimilation rate (NAR). Changes in antioxidative enzymes caused by flooding applied at vegetative stage were reversible. In generative plants, antioxidative enzymes were activated to a greater extent during recovery period and in contrast to vegetative plants did not attain the control level after that period. The reduction in growth was greater when flooding stress was applied at vegetative stage. Vegetative plants regardless of the stabilisation of antioxidant system were not able to restore the physiological function to attain the optimal growth level after flooding. Probably other injuries caused by flooding had a dominant effect on enzymatic antioxidant changes during stress period.     

Evaluation and development of flood‐tolerant soybean cultivars

Soybean (Glycine max [L.] Merr.) cultivars are generally sensitive to flooding stress. The plant growth is severely affected and grain yield is largely reduced in the flooded field. It is important to develop flood‐tolerant soybean cultivars for grain production in regions of heavy rainfalls worldwide. In this study, a total of 722 soybean genotypes were evaluated for flooding tolerance at R1 stages (first flower at any node) in the 5‐year flooding screening tests. Differential soybean genotypes exhibited diverse responses to flooding stress with that plant foliar damage score (FDS) and plant survival rate (PSR) ranged from 1.9 to 8.8 and 3.4% to 81.7%, respectively (p < .0001). Based on our standard of flooding evaluation, most genotypes were sensitive to flooding with 6.0 of average FDS and 38.7% of PSR. Fifty‐two soybean genotypes showed flooding tolerance and 11 genotypes were with consistent flooding tolerance during 4‐ to 5‐year continual evaluations. In the meantime, six genotypes were identified with consistent high sensitivity to flooding. The group analysis showed that genotypes from different sources had distinguishable responses to flooding stress (p < .0001). The interacting analysis of year and flooding tolerance indicated that FDS and PSR means were significantly different among 5 years due to weather temperature and flooding treatment time influences of each year (p < .0001). Furthermore, five breeding lines with high‐yielding and flood‐tolerant traits were developed using selected consistent flood‐tolerant and high‐yielding genotypes through conventional breeding approach.     

持续淹水对小麦养分吸收动态和产量的影响

为了研究持续淹水对小麦养分吸收动态和产量的影响,以沿淮低洼地砂姜黑土上的小麦为研究对象,共设6个处理,每个处理埋相应的根袋,在小麦生拔节期持续淹水9天,每天收取根袋,淹水结束后统一采集各处理的植株样。淹水严重影响了小麦的生长发育,降低生物量,减少产量,淹水时间越长,下降和减产也越严重;持续性淹水严重阻碍了小麦对氮、磷、钾的吸收,降低了地上部分氮素含量和累积量,经t检验与对照相比差异均达显著或极显著水平。淹水严重影响了小麦的生长发育,抑制了氮、磷、钾的吸收与积累,使生物量、产量有较大幅度的下降。     

Cover Crops for Saline Soils

A 3-year study was conducted in the Central Valley of California to evaluate 125 prospective winter-growing cover crops for growth and nitrogen productivity in saline soils. Soil saturation paste electrical conductivities (ECes) in the surface 15 cm averaged 7 dS  m⁻¹ at fall planting and 5.3 dS m⁻¹ at spring harvest dates of each experiment. Species evaluated varied substantially in plant height. In general, the tallest plants were the Brassica species, which consistently grew to over 1.4 m. Annual grasses (barley, rye, triticale and wheat) averaged about 1.0–1.3 m in each year. Of the legume species screened, heights were greatest for Hedysarum coronarium , Trifolium alexandrium , Vicia spp., and Medicago polymorpha and truncatula , averaging 59, 47, 39, 38 and 37 cm, respectively, over all experiments. About one third of the species screened produced crop cover in excess of 90 % in each year. Groups of plants with consistently high crop cover percentages included various species/accessions of Brassica , Hedysarum , annual grasses, cool-season annual medics, Medicago polymorpha and Medicago truncatula , and two annual clovers, Trifolium alexandrium cv 'Multicut' and rose clover, Trifolium hirtum cv 'Hykon.' Total above-ground plant dry weights were highest for Brassica spp., which produced twice as much biomass as the annual grass species, and roughly four times as much dry matter as any of the legume species. Hedysarum , Lana and Namoi woolypod vetch, purple vetch, berseem clover, and several of the annual medic species consistently had the highest biomass among the legumes.     

The dynamics of lucerne (Medicago sativa L.) yield components in response to defoliation frequency

The productive life of lucerne (Medicago sativa L.) stands depends on the rate of mortality of individual plants. However, self-thinning of plant populations may be compensated for by increases in other yield components, namely shoots/plant and individual shoot mass. Frequent defoliation reduces lucerne yield but it is unclear whether this is caused by an acceleration of plant mortality or changes in these other yield components. To investigate this, crops with contrasting shoot yields were created using constant 28 or 42-day regrowth cycles applied to a ‘Kaituna’ lucerne crop in Canterbury, New Zealand during the 2002/2003 and 2003/2004 growth seasons. Two further treatments switched from 28 to 42 or 42 to 28 days grazing frequency in mid-summer (4th February) of each year. The annual yield of shoot dry matter (DM) ranged from 12 to 23 t/ha for the treatments defoliated consistently each 28 or 42 days, respectively. Plant population was unaffected by treatments and declined exponentially from 130 plants/m² in June 2002 to 60 plants/m² in September 2004. The dynamics of plant and shoot population were associated with the light environment at the base of the canopy. The slope of the size/density compensation (SDC) of plants was −1.67 for the treatment defoliated each 42 days, near the expected self-thinning slope of −1.5 for stands at constant leaf area index (LAI). Self-thinning of shoots resumed after each defoliation when the LAI reached 2.1 and the transmission of photosynthetically active radiation (PAR_t) was 0.20. At this point the proportion of aerial DM in the tallest (dominant) shoots increased non-linearly from 30 to >80%, due to the mortality of intermediary and suppressed shoots. The average maximum shoot population in each rotation was 780 shoots/m² and unaffected by the decline in plant population due to a compensatory increase from 6 to 13 shoots/plant as the stand thinned. A lower asymptote of 43 plants/m² was estimated as the minimum plant population at which yield component compensation would maintain the productive potential of these ‘Kaituna’ stands. Differences in shoot yield were explained (R² = 0.97) by changes in the individual shoot mass (ISM) that were consistently lowered by frequent defoliation treatments. Frequent defoliations reduced crop productivity by limiting the assimilation of biomass into each individual shoot with negligible impact on shoot appearance rate, the number of shoots per plant at an LAI of 2.1 or the rate of plant population decay. Inter-specific competition for light was proposed as the main factor controlling self-thinning of plants and shoots regardless of their individual C:N status.     

Accurate evaluation and verification of varietal ranking for flooding tolerance at the seedling stage in barley (Hordeum vulgare L.)

Soil flooding or waterlogging is a major abiotic stress in upland crops. In barley, there have been several reported studies of selection for flooding-tolerant genotypes, but it is difficult to obtain varietal rankings that are consistent among researchers. Our objectives were to establish experimental conditions that could be applied by other research groups and to verify the varietal ranking conducted in an earlier study. We conducted greenhouse experiments on 14 barley varieties. At the 2.5-leaf stage, they were flooded with 0% or 0.1% soluble starch solution (mimicking reducing conditions). At 13 to 15 days after the start of treatment, the degree of leaf injury and the shoot dry weight ratio (treatment:control) were recorded. Reliable and highly repeatable results were obtained for the criterion of leaf injury under reducing conditions, whereas shoot dry weight ratio was unstable. The varieties OUJ820 and OUA301 were highly tolerant, whereas OUA002 and OUJ247 were sensitive; these results matched those of the earlier study. The experimental conditions that we developed here may be useful for selection testing and genetic analysis of flooding tolerance in other laboratories.     

Effects of Different Tree Species on Growth and Yield of Mung Bean (Vigna radiata (L.) Wilczek) Grown in Hedgerow Intercropping Systems in Sri Lanka

Reduction of crop yields due to resource competition from tree hedges is a serious drawback of hedgerow intercropping. This work quantified the competition of six potential hedgerow tree species ( Calliandra calothyrus , Desmodium ransonii , Flemingia congesta , Gliricidia sepium , Cassia spectabilis and Tithonia diversifolia ) on mung bean ( Vigna radiata ) grown as hedgerow intercrops at Pallekelle in the mid-elevational (367 m above sea level), subhumid (rainfall of 1400 mm year⁻¹) zone of Sri Lanka. Leaf area, total biomass and yield of mung bean in hedgerow intercrops showed a clear reduction closer to the hedgerows, whereas no such reduction was observed in a sole crop of mung bean. At 30 cm from the hedgerow, growth and yield of mung bean were significantly lower than in the sole crop, indicating significant competition from hedgerows. However, growth and yield of mung bean increased with increasing distance from hedgerows so that, at 150 cm, mung bean under Gliricidia and Desmodium showed significantly greater growth and yields than the control. In contrast, even the maximum mung bean yields under the rest of the species did not reach that of the control. The differences in competition with different hedgerow species are discussed in terms of variations between tree species in biomass production, quality of prunings and the possibility of root competition.     

Soya Bean (Glycine max L. Merr.) Genotype Response to Early-season Flooding: II. Aboveground Growth and Biomass

Soya bean is often grown in regions prone to periodic flooding, thus selecting cultivars that maintain production under waterlogged conditions is desirable. An experiment involving flooded soya beans was planted in southern Florida to examine (1) stem and leaf growth; (2) morphological adaptations; and (3) the relationship between early‐season and late‐season flood tolerance in flooded soya beans. Eleven soya bean genotypes previously defined as tolerant or sensitive to flooding were subjected to three treatments at 21 days after sowing (DAS): (1) no flood, (2) 2‐week flood and (3) 4‐week flood. All plants were harvested 49 DAS. Flooded plants exhibited lower stem dry weights but greater partitioning to the stem. Non‐flood treatments had greater leaf dry weight, leaf area and partitioning to leaves than flooded plants. There were positive correlations of genotype stem dry weight and leaf dry weight to early‐season flood tolerance but stem partitioning was negatively correlated with early‐season flood tolerance. Genotypic rankings of early‐season flood tolerance in this study were not correlated with earlier studies basing flood tolerance on seed yield. Our study highlights the range of soya bean morphological adaptations in response to flood. However, our results indicate that early‐season screening may not be an accurate predictor of soya bean genotypic response to late‐season flood.     

Varietal variation and physiological basis for inhibition of wheat seed germination after excessive water treatment

Soaking the seeds of most upland plants in water before sowing results in poor germination. Varietal differences in flooding tolerance of seeds have been reported in maize, soybean, barley and so on. This study was conducted to evaluate the varietal difference in wheat (2n = 42) seeds to soaking injury and to examine the importance of ethanol accumulation and seed coat as determinants of flooding tolerance. Of 342 varieties tested, many from Asia appeared more tolerant of flooding than the varieties from the Middle East. Soak-induced inhibition of germination and amounts of ethanol excreted were increased with soaking duration. Seeds of 26 wheat varieties were soaked 8 days at 20 °. Subsequent germination was correlated with amounts of ethanol excreted. Seeds with a red-colored coat exhibited higher tolerance to flooding than white ones, however, a varietal difference existed even in the case where the seed coat was peeled. These data suggest that soak-induced inhibition of wheat seed germination relates to accumulation of ethanol rather than seed coat color. This revised version was published online in July 2006 with corrections to the Cover Date.     

Gaseous Loss of Nitrogen in Paddy Soils

Irrigation practices for rice ( Oryza sativa ) that are common in South Asia were simulated under greenhouse conditions, and the resulting range of gaseous N losses was determined. The interaction of water and fertilizer management for growing paddy rice was quantitatively evaluated at several rates of N fertilization. Continuous flooding, alternate flooding and drying, and dry periods before, after, and during flowering were combined with 3 rates of N, i.e., 0, 50, and 100 mg kg⁻¹ by uniform incorporation and by mud ball placement.
Gaseous loss of native soil N was small (10 mg kg⁻¹) when no fertilizer was applied and the crop stand was adequate. Loss increased to 32 mg kg⁻¹ when stands were halved. Gaseous loss of 41 to 58% were found when fertilizer was applied. Alternate flooding and drying was the least desirable irrigation practice with losses of 40 to 87 mg N kg⁻¹. Placement of N in mud balls reduced gaseous N loss under all irrigation procedures by 14 to 32% compared to uniform application at the low N application rate. Under continuous flooding only 28% of a second increment of 50 mg kg⁻¹ of applied N was lost compared to 82% loss with alternate flooding and drying.     

Agronomic and Physiological Study of Cold and Flooding Tolerance of Spelt (Triticum spelta L.) and Wheat (Triticum aestivum L.)

Flooding of soil occurs all over the world in areas of crop production. The resulting lack of available oxygen in the soil can hinder the establishment of field crops such as wheat ( Triticum aestivum L.) and thus lead to a decrease in yield. The current study compares the tolerance of spelt ( Triticum spelta L.) and wheat to wet and cold stress during germination and early growth. Two specific traits seem to be related to the superior early flooding tolerance of spelt: (1) fast growth of the coleoptile, which reaches the soil surface rapidly, thus reducing the time span of hypoxia sensitivity, independent of temperatures between 10 and 20 °C; (2) the improved physiological adaptation for coleoptile growth under hypoxia is probably due to lower oxygen consumption between germination and emergence. Because of this high level of flooding tolerance, spelt would also be a good source for breeding for stress tolerance in wheat. These findings will help to detect quantitative trait loci (QTL) for characters such as growth rate, flooding tolerance and oxygen consumption in future studies. A marker-assisted introgression of spelt into wheat is necessary to avoid combination with undesirable agronomic traits of spelt.     

Responses of Root Nodule Formation and Nitrogen Fixation Activity to Nitrate in a Split-Root System in Peanut (Arachis hypogaea L.)

Peanut is an important constituent of crop rotation systems with various vegetable crops, which need large amounts of inorganic nitrogen. Nitrate inhibits nodule formation and nitrogen fixation of the peanut plants in these cropping systems. To elucidate this nitrate-induced inhibition, an experimental method using a split-root system was designed and the inhibitory level of nitrate (14 m M ) was applied to half of the roots for 5 or 30 days in pot experiments. In the application of nitrate for 5 days, the dry weight, the nitrogen content, and both the number and the fresh weight of nodules for each half of the roots were unaffected by the nitrate applied to the other half. However, the nitrate induced significantly lower nitrogenase activity in the applied half. In the application for 30 days, nitrate induced a significantly higher nitrogen content in the applied half of the roots, and the inhibitory effect on nodulation and nodule development was observed not only in the applied half but also in the other half. Thus, the inhibitory effect of long-term nitrate application on the nodulation and nitrogenase activity of peanut may be systemic, but that of short-term nitrate application, in which external nitrate may regulate nitrogen fixation, may not be systemic.     

Effect of Temperature and Photoperiod on the Development of Lupinus albus L. in a Controlled Environment

An experiment to determine the effect of temperature and photoperiod on Lupinus albus under controlled environmental conditions was carried out, using the three Lupinus albus genotypes 'Tifwhite', 'Esta' and 'Kiev', and three temperature (10/20, 18/28 and 20 °C continuously) and two photoperiod (8 and 16 h daylength) regimes, in all combinations. Half of the seeds were vernalized for 21 days at 4 °C to alleviate the obligate vernalization requirement of Tifwhite. Although Esta and Kiev do not have obligate vernalization requirements, they were influenced by this vernalization period. Observations included the duration of the period from planting to seedling emergence, the duration of the period from planting to the beginning of flowering and the duration of flowering. The vernalization treatment accelerated plant development in all genotypes. The period from planting to emergence was shorter under the higher temperature regime. For all genotypes, the period from planting to flowering was shorter under the longer photoperiod, the same trend as would be expected for long-day plants. Duration of flowering periods were, in contrast to pre-flowering periods, shorter for all genotypes at cooler temperatures. The results of this study confirm that photoperiod does contribute to the growth period from planting to flowering in L. albus and that this species does behave as a long-day plant.     

Effects of Irrigation at Different Growth Stages on Vegetative Growth of Mung Bean, Vigna Radiata (L.) Wilczek, in Dry and Intermediate Zones of Sri Lanka

Mung bean crops in the subhumid zones of Sri Lanka experience significant drought periods. The objective of this study was to quantify the growth response of mung bean to irrigation at different phenological stages and thereby determine the optimum irrigation regime to maximize growth. Four field experiments were conducted at two sites in 1995 and 1996. The crop duration of mung bean was divided into three stages: vegetative (from germination to appearance of first flower), flowering (from appearance of first flower to 75 % pod initiation) and pod-filling (from 75 % pod initiation to maturity). Eight treatments were devised to represent all possible combinations of irrigation at the three stages. Maximum leaf area index (ranging from 0.6 to 2.6 across treatments) and total leaf area duration were increased significantly by irrigation during the vegetative stage. Specific leaf weight decreased and maximum total crop biomass (150–400 g m⁻²) increased with the number of stages irrigated. Irrigation decreased the absolute root biomass and increased the shoot:root ratio. It is concluded that, in this agroclimatic zone of Sri Lanka, irrigation of mung bean during the vegetative stage is critical for maximizing leaf area. However, biomass production can be maximized by increasing the number of stages irrigated irrespective of irrigation at any specific stage.     

Evaluation of CropSyst for simulating the yield of flooded rice in northern Italy

Research on rice cropping systems carried out in Europe has to face the great variability of pedo-climatic conditions, and the linked abundance of cultivated varieties, characteristic of the high latitudes-temperate areas where rice is traditionally grown.

Dynamic simulation models can provide an useful tool for system analysis needed to improve the knowledge, the agronomic management and crop monitoring.

For calibrate and validate CropSyst (never used for rice), a process-based simulation model, for Indica-type and Japonica-type varieties, data obtained from five field experiments, carried out in Northern Italy between 1989 and 2002, were used.

Plants were sampled during the life cycle from rice plots of five cv Loto, Cripto, Ariete, Drago, Thaibonnet and Sillaro, maintained at potential production, to determine some important crop variables and parameters such as aboveground biomass (AGB), leaf area index, specific leaf area, harvest index, the date of the main phonological stages.

At the end of the calibration process to the parameters (the others were set to the default value, taken from the Literature or measured) optimum mean daily temperature for growth, specific leaf area (for Japonica varieties), stem/leaf partition coefficient (empirical), leaf duration, were assigned the following values: 28 and 27 °C respectively for Japonica and Indica varieties, 27 and 29.5 m² kg⁻¹ respectively for Japonica early and medium-late varieties, 4.5, 3, 1.5 for Japonica early, medium-late and Indica varieties, 700, 850, 950 °C-days for the three groups of varieties.

The assessment of model performances has shown average RRMSEs of 20 and 22% at the end of calibration and for the validation process; the modelling efficiency is always positive and the coefficient of determination always very close to 1. General improvements will be achieved by the model by considering the thermal profile (strongly influenced by flooding water at mid latitudes) evolving in and over the canopy.     

Analysis of the Impact of Alfalfa Forage Production under Summer Water-Limiting Circumstances on Productivity, Agricultural and Growers Returns and Plant Stand

The trade-off between summer water conservation from alfalfa production and the effects on plant growth, agricultural crop value, and grower returns in the low desert area (Imperial, Palo Verde, Parker and Yuma Valleys of California and Arizona) was analysed using an experimental plot in the Palo Verde Valley. The purpose was to provide a basis for evaluating water conservation decisions and policies. Four summer dry-down periods (withdrawing water for a predetermined period) of 0 days (control), 35 days, 70 days, and 105 days were analysed. Our results show that whereas the various dry-down periods would provide water conservation ranging from 254 to 944 million m³, the region would experience agricultural income decline by US $16 to US $73 million. For growers, benefits of dry-down would be effective only if the water price exceeds US $0.045 m⁻³ in California and US $0.036 m⁻³ in Arizona. Also dry-down would have possible adverse effects in the following areas including declines in plant stand density, long-term productivity, service industry sales, employment and biological benefits of soil fertility and organic matter that is attributed by the alfalfa plant in which case agriculture's and growers monetary losses could increase.     

Effect of Climatic Variation on Yield of Sesame (Sesamum indicum L.) at Different Dates of Sowing

Although experiments have previously been carried out to determine the optimum sowing time of sesame, very few attempts have been made to determine the effects of macro and micro climatic variation on yield. Thus a field experiment was conducted at the University Research Farm, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, India on an Entisol (alluvial, sandy loam) in the summer seasons of 1996 and 1997 to assess the effects of macro and micro climatic variations on yield of sesame for various dates of sowing. The number of seeds per capsule of sesame was highest in the crop sown on 19 February, although up to the 18 April sowing there was no significant variation. There was no appreciable variation in thousand-seed weight with date of sowing. Crops sown on 19 February and 1 March produced statistically similar yields. The average reduction in yield of sesame was 78.5 and 213 %, respectively, for crops sown on 10 February and 28 April, compared with the crop sown on 19 February. The cultivar Rama produced higher yields than B-67 and Kanke-1. Ambient temperature above 30 °C up to 100 % flowering had a direct positive effect on sesame yield. Crop exposure to 60–70, 70–80 and  80 % relative humidity up to 100 % flowering had a direct negative effect. Temperature profile at 50 days after emergence (DAE), relative humidity at 60 DAE, and photosynthetically active radiation (PAR) at 30 and 40 DAE within the crop canopy had direct positive effects on yield. Regression models suggested that temperature profile and PAR within the crop canopy produced 72 and 35 % variation in yield, respectively.     

QTL analysis of flooding tolerance in soybean at an early vegetative growth stage

Soybean cultivars are sensitive to flooding stress and their seed yields are substantially reduced in response to the stress. This study was conducted to investigate the genetic basis of flooding tolerance at an early vegetative growth stage. Sixty recombinant inbred lines derived from a cross between a relatively tolerant cv. ‘Misuzudaizu’ and a sensitive cv. ‘Moshidou Gong 503’ were grown in pots in a vinyl plastic greenhouse in 2002 and 2003. At the two‐leaf stage, half of the pots were waterlogged by water placed in plastic containers and adjusted to 5 cm above the soil surface. After 3 weeks of treatment, the pots were returned to the greenhouse and grown until maturity. Flooding tolerance was evaluated by dividing the seed weight of the treated plants by that of the control plants. Quantitative trait loci (QTL) analysis using 360 genetic markers revealed three QTLs for flooding tolerance, ft1 to ft3 in 2002. The ft1 (molecular linkage group C2) was reproducible and an additional four QTLs, ft4 to ft7, were found in 2003. The ft1 had a high LOD score in both years (15.41 and 7.57) and accounted for 49.2% and 30.5% of the total variance, respectively. A large QTL for days to flowering was consistently observed across treatments and years at a similar position to ft1. Comparing the relative location with markers, the maturity gene probably corresponds to E1. Late maturity may have conferred a longer growth period for recovery from flooding stress.