温度对紫椴种子萌发及萌发过程中物质转化的影响

为研究经层积处理解除休眠的紫椴种子适宜萌发的温度条件,及不同温度条件下种子萌发过程中储藏物质的动态变化,开展了紫椴在5种恒温和4种变温下种子的发芽率和发芽指数的研究,并选择萌发最适与最差温度重新进行发芽试验,探讨2种温度对种子萌发过程中储藏物质的转化的影响。结果表明：紫椴种子适宜萌发的恒温条件为10℃和变温条件为15℃/10℃,温度高于20℃时,温度越高,萌发率越低。不同温度下储藏物质转化研究表明,高温下,淀粉酶活性在一定程度上受到抑制,导致种子中的淀粉分解缓慢,可溶性糖和蛋白代谢也受到不同程度的抑制,从而导致种子不能正常萌发。     

Hybrid Tea-rose pollen. I. Germination and storage

Summary Germination and storage trials were carried out with pollen of several rose varieties. The pollen grains germinated well in a 15% sucrose solution with 40 ppm boric acid. Staining the pollen with a 0.1% tetrazolium solution and standardizing the degree of colour at which the pollen grains are counted as viable, provided a good viability estimate, simpler to carry out than in vitro germination. Germination capacity and staining ability of the pollen were greatly impeded-about halved-by dehydration during storage in desiccators at low humidity. This effect could be corrected by humidifying the pollen beforehand for about one hour, though this pre-treatment increased the percentage of germinated pollen grains more than the percentage stained. There was no difference between the two percentages in fresh or in deep-frozen pollen.Pollen stored at 1°C and high relative humidity soon lost its germination capacity: between 0 and 20% humidity a considerable proportion of the pollen remained viable for 9 months and longer. Storage for the same period in vacuum-sealed glass tubes at –24°C maintained viability as well or better and would probably prolong it further. Some of the cold-stored pollen induced a reasonable seed set after one year, a low seed set was obtained even after two years of storage at 1°C and low humidity.     

傣药倒心盾翅藤种子萌发特性初步研究

目的:研究倒心盾翅藤种子的萌发特性。方法:测定倒心盾翅藤种子的形态、千粒重和含水量等指标,利用不同温度和光照、种子带翅或不带翅、不同成熟度、贮藏等处理对种子进行发芽试验,测定其发芽率、发芽势和发芽指数。结果:倒心盾翅藤种子发芽适温为25～35℃,其中30℃条件发芽率最高,光照或黑暗条件均可。果翅对种子的发芽率无显著影响,但会影响种子发芽速度和整齐度。不成熟种子采集后经过贮藏后熟,可显著提高发芽率。种子在室温条件下贮藏6个月,发芽率仍高达93.68%,但贮藏12个月后发芽率显著下降。结论:倒心盾翅藤种子属于高温萌发型、光中性种子;种子最适贮藏时间不超过1年。     

Effects of Thermal and Salt Treatments during Imbibition on Germination and Seedling Growth of Sorghum at 42/19 °C

This investigation tested the influence of thermal treatment of seeds at various stages of imbibition on the germination percentage, speed and growth response of seeds and seedlings. Seeds of sorghum (Sorghum bicolor L Moench) were treated in 2, 4 or 6 g NaCl l^?1 solutions and exposed for 2 h to 45 °C during the first, second or third days of imbibition. Thereafter, the seeds were dried and germinated at 42/19 °C (day/night temperature). Salt treatments did not improve the final germination percentage but increased germination speed over untreated seeds. The higher the salt concentration used, the greater the dry weights of plumules and radicles. Thermal treatment on the third day of imbibition yielded higher germination percentages than untreated seeds, while thermal treatment on the second day gave faster germination. Both the second and third day thermal treatments gave superior germination indices and higher plumule‐to‐radicle ratios. It is concluded that thermal treatment may assist in acclimating seeds to heat stress.     

Compatibility and incompatibility in witloof-chicory (Cichorium intybus L.). 1. The influence of temperature and plant age on pollen germination and seed production

Summary For the production of inbred lines and F₁ hybrids in witloof-chicory information is wanted on characteristics such as the incompatibility system. These characteristics can only be studied properly if the influence of temperature and physiological status of the plant on pollen germination and seed production is known. Investigations were carried out with 9 self-incompatible (SI) and 6 self-compatible (SC) clones in glasshouses of the IVT phytotron at constant temperatures of 10, 14, 17, 20, 23 and 26°C. In general, in vivo pollen germination percentages were rather low after self pollination with an optimum for germination around 17–20°C. No seeds were formed at the lowest temperature (10°C) while seed production for SC clones was usually (rather) good at higher temperatures. At 26°C seed production in some clones decreased. Both pollen germination and seed production decreased at the end of the flowering period. There was a rather positive relationship at e.g. 17 and 20°C between pollen germination after selfing and seed production. When no pollen germination was observed, no seed formation occurred. When pollen grains did germinate, seed development would not necessarily occur in all cases. So this relationship only enables negative mass selection for SC.     

The germination of freshly harvested seed of ripe and unripe barley and oats

Barley and oats were grown in a greenhouse and outside in the field to different stages of ripeness. After harvest the plants were threshed as soon as possible. The seeds were treated in different ways and sown immediately in a sand-bed. The kernels which germinated were counted.Seeds from plants which were grown in the greenhouse, on average germinated better than seeds from plants grown outside. Seeds from ripe plants germinated better than green seeds, but in some trials germination of green seeds was exceptionally good. The percentage of germination was increased by several treatments. Drying at 50°C was found to be the most efficient method.The result from such experiments may be different when the plants are grown in another environment. Under our conditions drying at a temperature of about 50°C was an efficient way to increase the germination of freshly cut seeds of barley and oats.     

Effects of desiccation and a change in temperature on germination of immature grains of wheat (Triticum aestivum L.)

A study was made of the effects of desiccation and a change in temperature on the germination of wheat grains harvested 20 days after anthesis. When the germination test was performed immediately after harvesting, germination percentages ranged from 5.2% to 10.7%. Germination percentages increased to 48.2% to 90.3% after grain had been desiccated at 20 °C and then hydrated at 10 °C. This increase occurred even if grains had been desiccated in an atmosphere of high relative humidity. The germination percentage of non-desiccated grains depended on the germination temperature. When the pericarp and testa were removed from embryos, the germination percentage of grains incubated at 20 °C and then at 10 °C was higher than that of grains incubated at 10 °C. In general, a low germination temperature is believed to be effective in breaking dormancy of wheat grains. However, a change in temperature stimulated germination to a greater extent than a constant low temperature. The germination percentage of 5-day cycle alternating temperature was greater than that with 1-day, 2-day and 10-day cycles. Although the germination of immature wheat grains was stimulated by both high and low germination temperatures, it is likely that cycles shorter and, also, longer than a critical period induce limited germination. Loss of dormancy commonly occurs when development of wheat grains proceeds at a high temperature, with imbibition at a low temperature. However, germination ability of non-desiccated immature wheat grain was enhanced by a change in temperature during germination. This revised version was published online in August 2006 with corrections to the Cover Date.     

贮藏温度和时间对金铁锁种子萌发的影响

目的:为探讨金铁锁种子适宜的贮藏技术,对不同温度贮藏条件下的金铁锁种子做发芽试验.方法:将金铁锁种子采集后晒干,放置在4,20℃和常温条件下贮藏,在贮藏8个月、10个月和1 2个月时,取出种子做发芽试验.结果:贮藏8个月时,20℃和常温贮藏条件的发芽势分别为56.67％和53.33％,与4℃贮藏条件的发芽势(27.92％)差异显著,发芽率以20℃贮藏最高(83.75％),与4℃和常温贮藏差异显著;贮藏10个月时,常温贮藏的种子失去发芽能力,发芽率为0,4℃和20℃贮藏的发芽势和发芽率无显著差异;贮藏12个月时,4℃贮藏的发芽率最高为71.25％,与20℃贮藏的发芽率差异显著.结论:金铁锁种子应分开存放,对于存放时间超过10个月的种子,可以采用4℃低温冷藏的方式进行,对于存放时间不超过8个月的金铁锁种子,可以在常温下进行贮藏.     

Effects of water supply methods and seed moisture content on germination of China aster (Callistephus chinensis) and tomato (Lycopersicon esculentun Mill.) seeds

Uniform and fast germinating seeds are of prime importance for agriculture. To improve the germination properties of seeds, different treatments called priming are used. These methods generally involve the controlled uptake of water by the seeds. To be able to understand the processes involved in different priming methods the aim of the research was to study the relationship between the methods of water supply, water uptake rate by seeds, seeds moisture content and germination of China aster ‘Jolanta’ and tomato ‘Janosik’ seeds. Seeds of these species were primed by: (i) soaking in excessive amount of distilled water for 0–1440 min in the ratio of seeds to water (v:v) of 1:3; (ii) soaking in limited amount of distilled water 10–1280 ml kg⁻¹of seeds; (iii) matriconditioning in the ratios of 1:0.4:0.2–2.0 (w:w:w) for seeds, Calflo and water, respectively. The imbibed seeds were incubated for 1 day at 20 °C and seed moisture content was measured after incubation. Additionally, in seeds moistened up to 37% (China aster) and 35% (tomato), dynamics of water uptake were examined. Seeds, after imbibition and incubation, were dried to the initial moisture content (m.c.). Percentage of the germinated seeds, maximum germination (G_max), time for the first seed to germinate (T₁), time to reach 50% germination (T₅₀), time between 10 and 90% of G_max (T₉₀–T₁₀), at 5, 20 or 35 °C for the China aster seeds and at 15, 20 or 35 °C for tomato were evaluated.The results showed that water supply methods significantly affected seed imbibition rates and dynamics of seed germination, although these depended on plant species. It was found that water uptake was fastest in seeds soaked in excessive water amount and also that China aster took water faster than tomato. Imbibition of seeds during matriconditioning in Calfo and in limited water amounts was slower. Imbibition of seeds up to 37% m.c. for China aster and 35% m.c. for tomato, irrespective of the conditioning method, was the most favorable for germination. The earliest germination was observed in seeds soaked in excessive amount of water and incubated for 1 day. Germination of seeds matriconditioned, and those conditioned in limited water amount for 1 day was slower. We conclude that although the optimal final moisture content is independent of the different water supply methods used, the water supply method itself strongly affect the germination properties (T₁, T₅₀ and T₉₀–T₁₀ dynamics of germination).     

蒲葵种子的脱水耐性和贮藏特性研究

蒲葵种子自60～200 DAA(DAA表示花后天数),含水量和电导率逐渐降低,果实及种子千粒重、种子干重和生活力逐渐增大.130 DAA达到生理成熟,190 DAA完全成熟.60 ～ 190 DAA种子脱水耐性不断增强,190 DAA最大.蒲葵种子适宜萌发温度为20 ～ 35℃.含水量为20％和24％的种子分别在4℃和15℃下保存一段时间后,生活力逐渐降低,电导率却增加.     

Hydrotime Analysis of Ethiopian Mustard (Brassica carinata A. Braun) Seed Germination Under Different Temperatures

Ethiopian mustard (Brassica carinata A. Braun) is an oil‐seed species that has recently become an object of great interest as a promising crop for energy purposes (biodiesel and biolubricant production). In semi‐arid regions of South Italy, soil moisture at sowing time is often inadequate, delaying and reducing seed germination. In this study, the effect of reduced water potential on seed germination in three cultivars of B. carinata (ISCI 7, CT 180 and Sincron) was investigated in the laboratory. Germination behaviour at constant temperatures under low water availability was also analysed through the hydrotime model. Six water potentials (ψ_s) in PEG solution (0, ?0.2, ?0.4, ?0.6, ?0.8 and ?1.0 MPa) and three temperatures (10, 20 and 30 °C) were used for the germination tests. A thermo‐inhibiting effect was observed at the highest levels of water stress. The hydrotime analysis revealed that the increase of temperature to 30 °C reduced predicted hydrotime (θ_H) by hastening the rate of germination, but shifted median base water potential (ψ_b(50)) to higher values (less negative), whereas the lowering of temperature increased θ_H. These observations may have a great agronomic impact because although fewer seeds germinated at 30 °C at the highest levels of water stress, they may germinate faster in rapidly drying soil. However, genetic differences were observed among cultivars in terms of estimated θ_H and ψ_b(50). The hydrotime modelling approach offered in this study may help predict B. carinata seed germination under soil water deficit conditions occurring under early or late autumnal sowing.     

Interaction of Salinity and Temperature on the Germination of Sorghum

Laboratory studies were conducted to investigate the effect of salinity × temperature interactions on the germination of sorghum. Sorghum bicolor (L.) Moench. Sorghum seeds were sown in petri dishes with saline solutions of varying concentrations (electrical conductivities of 0.01, 6.4, 12.2, 17.4, 22.6, 27.2, 32.1, and 37.2 dSm^-1) prepared with NaCl. The germination responses of the seeds were determined over a wide range of temperatures (15, 20, 25, 30, 35 and 40 °C) for a period of 8 days. Salinity × temperature interactions were highly significant at each counting date. Although, increased salinity resulted in decreased germination percentages, the detrimental effect of salinity was generally less severe at higher temperatures. Germination rate index, computed from germination counts taken every 2 days, was influenced by salinity and temperature in a similar pattern as final germination. Seed germination was more tolerant to salinity at germination temperatures of 30–40 °C than at 15–25 °C.     

冬小麦籽粒发育过程中休眠性的变化

对六个冬小麦品种不同采收期的种子进行了水分,鲜干种子发芽及休眠性测定。结果表明;鲜种子的萌发率随要育程度而提高。在生理成熟前后达到最高值,并与其水分含量高度负相关,干燥可显著地促进示熟种了的萌发;种子的休眠性随发育程度的提高而减弱,１７ＤＡＡ以后采收的种子没有休眠性。     

冬小麦种子的发育及其田间活力

１９９３－１９９４年北京对六冬小麦品种籽粒发育程度及不同发育程度籽粒的发芽力、活力进行了研究。从花后５天开始每３天取样一次,测定籽粒的干重,观察了其中两种材料发育早期籽粒种胚的器官分化,对各发育程度种子进行了标准发芽测定及田间幼苗活力鉴定。     

壮瑶药野甘草种子萌发特性研究

研究壮瑶药植物野甘草种子萌发特性,为野甘草育种以及规范化种植提供科学依据。以野甘草种子为材料,测定不同温度和光照条件下种子发芽率,及聚乙二醇(PEG-6000)、赤霉素(GA)、硝酸钾浸种对种子萌发的影响。结果表明：（1）野甘草种子在20℃和35℃恒温条件下不萌发,25℃、30℃、15/25℃变温、20/30℃变温和25/35℃变温条件的发芽率分别为78%、43%、73%、94%和75%;（2）野甘草种子在全黑暗条件下不萌发,由黑暗转入光照后,种子正常萌发;（3）浓度为1%~5% PEG-6000、1~100 mg/L GA和1~5 mmol/L KNO₃浸种后,野甘草种子发芽率均显著高于蒸馏水对照处理,分别为86%~90%、93%~95%和87%~88%。野甘草种子为需光性种子,适宜发芽温度为20/30℃变温,PEG-6000(1%~5%)、GA(1~100 mg/L)和KNO₃(0.01~5 mg/L)浸种可促进野甘草种子萌发。     

Seed priming improves germination in saline conditions for Chenopodium quinoa and Amaranthus caudatus

Seed priming has proved to be an effective method in imparting stress tolerance to plants using natural and/or synthetic compounds to treat the seeds before germination. The present work aimed to evaluate the effectiveness of priming treatments in seeds of Chenopodium quinoa and Amaranthus caudatus to improve germination under NaCl. Species‐specific protocols for seed hydropriming and osmopriming were established by germinating seeds under different water potentials and creating seed imbibition curves. Primed seeds were then germinated under different concentrations of NaCl, and the effect of priming was analysed based on the parameters, such as final germination percentage (FGP), germination index (GI) and mean germination time (MGT). Seed hydropriming and osmopriming caused significant improvements in germination velocity and uniformity, reflected in high FGP, high GI and reduced MGT under salinity. C. quinoa had a higher tolerance to salinity than A. caudatus during seed germination. Improved germination in salinity resulted from osmopriming seeds with solutions of low water potential for A. caudatus, while for C. quinoa, this effect was achieved from hydropriming and osmopriming seeds with solutions of high water potential. Primed tolerance to moderate salinity was achieved for A. caudatus, and for both species, the salinity threshold for germination to occur was slightly broadened.     

The Effects of Depth and Duration of Burial on Seasonal Germination, Dormancy and Viability of Galium aparine and Bifora radians Seeds

The effects of depth and duration of burial on seasonal germination, primary/secondary dormancy, and viability of Galium aparine and Bifora radians were studied in two experiments. The seeds of both species were buried at different depths with four replications in a split‐plot design in the first experiment. The seeds were exhumed from the field at monthly intervals and germinated under suitable temperature conditions. In the second experiment, the seeds of both species were stored at 20 °C and 50 % relative humidity after harvest. Four replicates of seeds from each species were adjusted for germination tests at monthly intervals. Non‐germinated seeds of either the first or the second experiment were treated with triphenyltetrazolium chloride in order to enhance the viability of seeds at monthly intervals until the end of the experiment. The seeds of G. aparine showed cyclic behaviour in the burial experiment. The germination percentage of B. radians seeds never exceeded 8 % at all depths until 7 months after burial due to primary dormancy. Thereafter, the seeds showed typical cyclic behaviour with modest deviation. In both the species, the depth of burial strongly affected seed viability. In the dry storage conditions, the seeds of G. aparine and B. radians showed a high level of primary dormancy. The percentage viability of seeds declined depending on the duration of storage.     

In vitro germination and viability of buckwheat (Fagopyrum esculentum Moench) pollen

An in vitro method for the germination of common buckwheat pollen was developed. Pollen grains were successfully germinated in an artificial medium consisting of 0.2 g each of MnSO₄, Ca(NO₃)2.4H₂O and KNO₃, 0.04 g H₃BO₃, 15 g sucrose and 30 g polyethylene glycol (molecular weight approximately 20,000) dissolved in 100 ml of double distilled water. The viability of pollen was assessed by in vivo and in vitro germination tests at 20 °C and 25 °C over a 38 h time period. Pollen grains were collected and germinated at 4 h intervals from freshly harvested flowers grown under 16 h day length and a constant temperature. Maximum pollen viability was found 2 h and 6 h after first light when plants were maintained at 25 °C and 20 °C, respectively. Viability, as measured by germination percentage, was similar at both temperature regimes. Some pollen remained viable for approximately 34 to 38 h in intact flowers, but all pollen lost viability in less than an hour when stored at room temperature without humidity control. This revised version was published online in July 2006 with corrections to the Cover Date.     

Germination Responses of Cañahua (Chenopodium pallidicaule Aellen) to Temperature and Sowing Depth: A Crop Growing Under Extreme Conditions

Cañahua (Chenopodium pallidicaule) is grown in the Altiplano of Bolivia and Peru, between 3810 and 4200 m a.s.l. Rural indigenous households have cultivated the cañahua as a subsistence crop for millennia. The seeds have a high content and quality of protein. We studied the relation between the following: (i) temperature and seed germination and (ii) the effect of temperature and sowing depth on seedling emergence of five cultivars and one landrace. Three experiments were conducted as follows: (i) seeds of a cultivar were germinated in Petri dishes at six temperatures (3, 5, 10, 14, 20 and 24 °C), (ii) sown at five depths (0, 5, 10, 25 and 50 mm) in a mixed peat soil substrate at three temperatures and (iii) one landrace (Lasta) and 5 cultivars (Lasta and Saihua growth habit) were sown in 6 depth (0, 5, 10, 25, 35 and 50 mm) in a sandy loam at two temperatures (5 and 15 °C). Temperature had significantly effect on the germination percentages of the plants (P < 0.001). Seeds germinated at the lowest temperature (3 °C). The estimated base temperature was close to 0 °C. A polynomial function described well the relation between time to 50% germination (t₅₀) and temperature in the interval from 3 to 24 °C resulting in a linear relationship between germination rate and temperature. Shallow sowing depth (5–25 mm) resulted in 80% germination at 15 °C. There were significant differences of emergence in relationship to burial depth (P < 0.001). Only few seedlings emerged when seeds were sown at 50 mm depth. We did not find significant differences in emergence of seedlings between Lasta and Saihua at 15 °C. Nevertheless, at 5 °C, seedlings of cañahua belonging to the Lasta growth habit form did have higher germination rate as were shown for the Kullaca cultivar and the Umacutama landrace. This may be attributed to larger seed size of these cultivars.     

Rapid and Synchronous Germination of Cicer Milkvetch (Astragalus cicer L.) Seed following Diurnal Temperature Priming

Experiments were conducted to determine if diurnal temperature variation could be used to prime cicer milkvetch ( Astragalus cicer L.) seed and thereby shorten the time for seed germination. For untreated cicer miikvetch seed at constant 20°C, 50% of the seeds germinated in 8.2 days and 90% of the seeds germinated in 10.5 days. In contrast, seed that was first primed for 7 days with a diurnal temperature variation (35 (8h)/5 (16h) °C) had values of 1.9 days and 3.3 days, respectively. A second experiment evaluated priming of seed in bulk to test if a procedure might be developed that could be scaled up to prime commercial quantities of seed. A bulk sample of seed was soaked in water for 5h at room temperature and then kept in a high humidity atmosphere during 7 days of priming at 35/5°C. Hydrated seed from the bulk sample had 50% germination in 2.7 days and 90% germination in 5.3 days. When the bulk sample was dried for 8 days at room temperature, the benefit of priming on shortening germination time was retained. It is likely that diurnal temperature priming could be adapted to large volumes of seed.