Distribution and redistribution of mineral nutrients and dry matter in grain sorghum as affected by soil salinity

A study was made of the effects of soil salinity on dry matter production, grain yield, and the uptake, distribution and redistribution of mineral nutrients in irrigated grain sorghum. Soil salinity (EC, 3.6 mS/cm) reduced seedling establishment by 77%, and dry matter and grain yields per plant by 32%; grain yield/ha was reduced by 84%. Salinity reduced grain number per head, but not individual grain size. The accumulation of dry matter and most nutrients was reduced by salinity, but the distribution and redistribution of nutrients within the plant were largely unaffected. Redistributed dry matter provided 52 and 31% of the grain dry matter for control and salt‐affected plants, respectively. Salt‐affected plants had a greater proportion of their sulfur (S), magnesium (Mg), sodium (Na), and chloride (Cl) in stems and leaves than control plants at maturity. Grain had 50–90% of the nitrogen <N), phosphorus (P), S, and Mg, 20–50% of the potassium (K), manganese (Mn), zinc (Zn), and copper (Cu), but < 20% of the calcium (Ca), Na, Cl, and iron (Fe) contents of the whole plant. Over 65% of the N and P, and from 20 to 30% of the K, S, Mg, Cu, and Zn was redistributed from the stem and leaves to grain. There was no redistribution of Ca, Na, Cl, Fe, and Mn. Leaves were more important than the stem as a source of redistributed N, but the leaves and stem were equally important as sources of redistributed P, K, S, Mg, and Cu. Redistribution from the stem and leaves provided 80% of the K and 20–50% of the N, P, S, Mg, Zn, and Cu accumulated by grain. Concentrations of Na, and especially Cl, were high in vegetative organs of salt‐affected plants, but not in grain. It was concluded that although moderate salinity was detrimental to the establishment and yield of grain sorghum, it had little effect on patterns of distribution and extents of redistribution of mineral nutrients.     

Enhancement of salinity tolerance in tomato: Implications of potassium and calcium in flowering and yield

Abstract

Five tomato (Lycopersicon esculentum Mill) cultivars were grown in sand nutrient culture experiment in a greenhouse to investigate the effects of salinity on growth and yield. Nutrient solutions were made saline with 50 mM NaCl (EC = 5.5 mS/cm or supplemented with 2 mM KNO₃ (EC = 6.8), 20 mM Ca(NO₃)₂ (EC = 7.5), and combination of potassium (K) and calcium (Ca) (EC = 8.0). Seedlings were irrigated with saline treatments commencing two weeks after transplanting. Determination of sodium (Na) and K in tomato leaves and fruits were by flame photometry. Accumulation of Na in tomato fruits was higher than in leaves under control or saline conditions for all tomato cultivars. The amount of K in the tomato leaves was higher in control than in saline‐grown plants. Addition of K and Ca to the nutrient solution resulted in a 3 to 7 fold increase in K accumulation in all cultivars tested. Stem and leaf growth were significantly reduced with salinity but growth was enhanced following irrigation when K was added to the nutrient solution. Flowering and fruit set were adversely affected by NaCl stress. Reduction of flower number was 44% relative to the control plants. Fresh fruit yield decreased by 78% when plants received 50 mM NaCl. Growth and development of tomatoes under saline conditions was enhanced in this study following the application of K to the saline nutrient solution. Amelioration in growth was also achieved when Ca was used but to a lesser extent. Our results suggest that ion accumulation and regulation of K and Ca contribute to salt tolerance and growth enhancement.     

Growth and macronutrient contents of faba bean plants: Effects of salinity and nitrate nutrition

The effects of the interaction between sodium chloride, nitrate, and concentrations on growth and internal ion content of faba bean (Vicia faba L.) plants were studied, to understand the relationship between the above parameters and salt tolerance. Increased salinity substantially reduced the dry weight of roots and shoots and increased the root/shoot biomass ratio. Additional nitrate‐N considerably moderated the salinity effects on these parameters. The promotive effects of nitrate‐N were more pronounced on shoot dry weight. These results suggest that an exogenous supply of nitrate‐N would improve the vegetative growth of V. faba plants by moderating the suppresive effects of salinity. The evolution of the root and shoot content in potassium (K), sodium (Na), magnesium (Mg), calcium (Ca), and nitrogen (N) was monitored during vegetative growth. A high correspondence between total N and Ca content was found. The acquisition of Ca and K in response to salt and nitrate was similar in shoots and roots, whereas Mg uptake showed notable differences in the two organs. In salt‐affected plants, the roots were found to be high in accumulated Na while the shoots exhibited the lowest Na concentration. Potassium accumulation was higher in the shoots. In this way, there was an antagonistic effect between Na and K uptake. Analyses of the nutrient contents in plant organs have provided a data base on salt‐tolerance mechanisms of V. faba plants.     

Salinity and mineral nutrition effects on growth and accumulation of organic and Inorganic ions in two cultivated tomato varieties

A trial was conducted on the effect of salinity and method of fertilizer application on two varieties of cultivated tomato, i.e. VF 145 and Edkawi. Salinity ranged from 0.52 to 11 dS/m, and fertilizer was applied by either broadcasting in small doses or added with irrigation water. Weight of shoots, fruit yield, and sodium (Na), calcium (Ca), chloride (Cl), free proline contents in both developing and mature leaves, and total soluble salts and ascorbic acid contents in fruits were taken as evaluating criteria. Salinity depressed both growth and fruit yield, and simultaneously increased ion concentration in plant leaves. Sodium and Cl accumulated with salinity, being greater in mature leaves, while proline accumulation in developing leaves was much higher than in mature leaves. Total soluble salts and ascorbic acid were not affected. Liquid fertilization resulted in higher fruit yields than that obtained with the solid fertilizer treatments as well as better alleviating the depressive effects of salinity on plant growth and yield, especially at the lower salinity level where it was more beneficial to fruit yield. The VF 145 tomato variety was found a bit more sensitive to salinity than the Edkawi variety, and was affected differently by salinity, regarding both the yield and the pattern of organic and inorganic ion accumulation. Our results suggest that there exists a physiological mechanism that is involved in the salt tolerance difference observed between the two varieties that needs to be investigated.     

Effects of Zinc Application on Growth,Absorption and Distribution of Mineral Nutrients Under Salinity Stress in Soybean (Glycine Max L.)

The purpose of the present work was to evaluate effects of zinc application on growth and uptake and distribution of mineral nutrients under salinity stress [0, 33, 66, and 99 mM sodium chloride (NaCl)] in soybean plants. Results showed that, salinity levels caused a significant decrease in shoot dry and fresh weight in non-zinc application plants. Whereas, zinc application on plants exposed to salinity stress improved the shoot dry and fresh weight. Potassium (K) concentration, K/sodium (Na) and calcium (Ca)/Na ratios significantly decreased, while sodium (Na) concentration increased in root, shoot, and seed as soil salinity increased. Phosphorus (P) concentration significantly decreased in shoot under salinity stress. Moreover, calcium (Ca) significantly decreased in root, but increased in seed with increased salinization. Iron (Fe) concentration significantly decreased in all organs of plant (root, shoot, and seed) in response to salinity levels. Zinc (Zn) concentration of plant was not significantly affected by salinity stress. Copper (Cu) concentration significantly decreased by salinity in root. Nonetheless, manganese (Mn) concentration of root, shoot, and seed was not affected by experimental treatments. Zinc application increased Ca/Na (shoot and seed) ratio and K (shoot and seed), P (shoot), Ca (root and seed), Zn (root, shoot, and seed) and Fe (root and shoot) concentration in soybean plants under salinity stress. Zinc application decreased Na concentration in shoot tissue.     

Response of two sorghum genotypes to soil lime and sodium chloride amendments

Abstract

Salinity affects plants by interaction between sodium (Na) and calcium (Ca). Two sorghum (Sorghum bicolor) genotypes ('Hegari’ and ‘NB‐9040') were studied for the Na x Ca interaction in a soil amended with 2% calcium carbonate (CaCO₃) and with 0, 12.3, 24.6, and 36.9 mmol sodium chloride (NaCl)/kg soil. The two genotypes were similar in their response to soil NaCl in their shoot and root growth but differed in response to lime. The salinity‐tolerant Hegari was suppressed by high Ca concentration in the soil, mainly in the low‐NaCl treatments, and responded by a lower concentration of potassium (K) and magnesium (Mg) in the leaves, which was associated with leaf‐chlorosis. Since Na uptake was reduced by Ca, the main effect of salinity on plant growth was by the accumulation of chloride (Cl) in the leaves.     

Interaction Between Salinity Stress and Verticillium Wilt Disease in Three Pistachio Rootstocks in a Calcareous Soil

ABSTRACT

The interaction between soil salinity and infection caused by Verticillium dahliae was studied in pistachio (Pistacia vera) in a greenhouse experiment. Treatments consisted of 0, 1400, 2800, and 4200 mg sodium chloride (NaCl) kg^{? 1} soil and three rootstocks (Sarakhs, Badami, and Qazvini cultivars). They were gradually exposed to salinity stress before and/or after root inoculation with a water suspension of 10⁷ conidia/mL of a pistachio isolate of V. dahliae. Salt stress significantly increased rootstock shoot and root colonization by V. dahliae. All rootstocks were susceptible to V. dahliae, but symptoms of the disease appeared earlier in Sarakhs, a salt sensitive cultivar. Moreover, salinity and V. dahliae interaction increased the concentrations of sodium (Na), potassium (K) and chloride (Cl), but decreased the K/Na ratio in all rootstocks. Shoot and root tissues of inoculated Sarakhs and Qazvini (a salt tolerant) contained the highest and the lowest concentrations of Na, K,and Cl, respectively. In salinity treatments, shoot and root dry weight of all rootstocks decreased as compared with controls. Sarakhs showed smaller shoot and root dry weight than Qazvini and Badami. Also, increasing the NaCl level increased accumulation of Na, K, and Cl in shoot and root of the rootstocks. Sarakhs showed higher concentrations of ions in the shoot and root. Based on shoot and root dry weights and ion accumulation, Sarakhs and Qazvini were susceptible and tolerant to salinity, respectively.     

Salt tolerance and mineral relations for celery

To invertigate the relationship between salt tolerance and plant mineral status in celery (Apium graveolens L.) growth and the concentration of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sodium (Na), and chloride (Cl) in different tissues were determined in plants grown in hydroculture with nutrient solutions containing 5 (control), 50,100, and 300 mM sodium chloride (NaCl) for four weeks. At salinity levels of 50 and 100 mM NaCl, there was a moderate, albeit significantly, reduction of growth, while a drastic decrease in both fresh and dry weight was obtained at 300 mM NaCl. Regardless of the salinity level, growth resumed promptly and completely once the stress was ceased. Sodium chloride stress reduced the accumulation of nitrate (NO₃)‐N in all plant tissues, but there were no relevant effects on the concentration of reduced N and P. The concentration of K in roots and leaf petioles was unaffected by NaCl treatment, but it gradually declined with increasing salinity in leaf blades. This reduction was less pronounced in the young leaves as compared to the mature ones. Increasing the NaCl concentration decreased the concentration of Ca in all tissues, but it prevented the occurrence of black‐heart, a typical Ca‐related physiological disorder which affected severely the controls. Salt‐stressed plants absorbed large amounts of Na and Cl which accumulated in the mature leaves, particularly in the oldest leaves. These findings suggest that the relatively high salt tolerance of celery relies on the ability to maintain an adequate nutritional status and to protect the shoot meristem from salt toxicity.     

Salicylic Acid–Induced Salinity Redressal in Hydroponically Grown Strawberry

The present study was conducted to evaluate shoot and root mineral composition of salt-stressed Selva strawberry under application timing of salicylic acid (SA). Treatments included plants sprayed with 0.5 or 1 mM SA, plants exposed to 40 mM sodium chloride (NaCl), and plants sprayed with 0.5 or 1 mM SA 1 week before, simultaneously, or after initiation of 40 mM salinity. Results indicated that under saline conditions, sodium (Na) and chloride (Cl) contents increased along with decrease in nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), and zinc (Zn) in shoot and root of plants. In plants treated with SA at 1 mM concentration, 1 week before salinity application, root Mg and shoot Ca were greater in comparison to salt-stressed plants treated with the same SA concentration 1 week after their exposure to salt stress. Thus, earlier SA application appears to be a better strategy for optimized protection against deleterious influence of salinity.     

Improved Nutrient Uptake Enhances Cotton Growth and Salinity Tolerance in Saline Media

Two experiments were conducted to determine if improved nutrient uptake increases salinity tolerance of cotton (Gossypium hirsutum L.). A transgenic cotton line (CMO3) with increased salt tolerance and its wild line (SM3) were grown in pots containing substrate (peat:vermiculite = 1:1, v/v) in the first experiment, while cotton (‘SCRC 28’) was cultured in hydroponics with a split-root system in the second experiment. Contents of essential nutrient elements and Na⁺ in plant tissues, leaf photosynthesis (Pn) and chlorophyll (Chl) concentration and plant biomass were determined after salinity [sodium chloride (NaCl)] treatment in both experiments. In the first experiment, salinity stress with 150 mM NaCl reduced plant biomass and photosynthesis (Pn) of both SM3 and CMO3 compared with their non-stressed controls, but the CMO3 suffered significantly lower reductions than SM3, suggesting an increased salinity tolerance of CMO3 relative to SM3. Total uptake and contents of main nutrient elements [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn)] in CMO3 were higher than those in SM3. Also, less sodium (Na⁺) accumulation and lower extreme ratios of Na/N, Na/P, Na/K, Na/Ca, Na/Mg, Na/Fe, Na/Mn, Na/Cu, and Na/Zn were observed in CMO3 than in SM3. Increased salt tolerance in transgenic AhCMO cotton was probably attributed to its superior nutrient uptake compared with SM3. In the second experiment, the non-stressed root half fed with moderate level of nutrient solution and salt-stressed half fed with low level of nutrient solution (CMN/SLN) exhibited higher salinity tolerance than salt-stressed root half fed with moderate level of nutrient solution and non-stressed root half fed with low nutrient solution (CLN/SMN). Plants absorbed more nutrients but less Na⁺ under CMN/SLN than CLN/SMN. The overall results suggest that improved nutrient uptake played an important role in the enhanced salt tolerance of cotton.     

Effect of sodium arsenite and sodium chloride on bean plant nutrition (macronutrients)

The effects of different levels of arsenic (As) and salinity on bean plant (Phaseolus vulgaris L., cv. Buenos Aires) nutrition were investigated. We studied the processes of absorption and accumulation of macronutrient elements: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg). The experiment was performed in soilless culture at two levels of As: 2 and 5 mg AsL^‐1 (added as sodium arsenite, NaAsO₂), and three saline levels [only sodium chloride (NaCl) was added]: 1, 2, and 4 dS‐m^‐1. Sodium arsenite and NaCl significantly affected macronutrients allocation within bean plant at concentration levels used in this study. Arsenite depressed K, Na, and Mg concentrations in root, whereas root N, and Ca levels were increased. Nitrogen, P, K, and Na concentrations were significantly higher in As‐stressed plants compared with controls. The addition of NaCl increased Ca concentration in roots and decreased that of K. Salinity tended to increase leaf concentrations of K, Na, Ca, and Mg; whereas leaf N and P levels decreased with increasing salinity.     

Influence of Nitrogen and Saline Water on the Growth and Partitioning of Mineral Content in Maize

ABSTRACT

Saline irrigation water has a tremendous impact on the yield potential of crops. Distribution of mineral elements in the parts of maize plant in response to saline water and nitrogen (N) nutrition was studied in a pot experiment for six weeks. Plants were irrigated either with tap water or saline water (ECw: 3.2 dSm^?1). Nitrogen was applied at the rate of 0, 50, 100 and 200-kg ha^?1 denoted as N0, N1, N2, and N3, respectively. Plants were separated into leaf, stem and root and analyzed for N, calcium (Ca), magnesium (Mg), sodium (Na), and potassium (K) concentrations. Dry matter production of leaf, stem and root was significantly reduced with saline water. The partitioning of elements in plants was the function of nitrogen and saline water. The N concentration of plant parts varied in the order of leaf > stem > root. A significant decrease in the N content was noted in plants under saline water. The root contained the highest Na content, Ca and Mg were higher in the leaf, whereas K was highest in the stem under saline water. Sodium was highest in the root and the remainder elements were greatest in the stem under tap water. Potassium and Cl were significantly reduced by N level whereas the reverse was true for Ca, Mg and Na content. The Na/K, Na/Ca, and Na/Mg ratios were also higher in salt stressed plant parts due to higher accumulation of Na ion. Among N-fertilizer treatments the Na/Ca and Na/Mg ratios were highest in control whereas Na/K increased with the addition of N. This study indicated that interaction of saline water and nitrogen has mixed effects on the partitioning of mineral elements in maize.     

Influence of Salinity on Chlorophyll,Leaf Water Potential,Total Soluble Sugars,and Mineral Nutrients in Two Young Olive Cultivars

The effect of salinity (NaCl) on chlorophyll, leaf water potential, total soluble sugars, and mineral nutrients in two young Iranian commercial olive cultivars (‘Zard’ and ‘Roghani’) was studied. One-year-old trees of these cultivars were planted in 10-L plastic pots containing equal ratio of sand-perlite mixture (1:1). Sodium chloride at concentrations of 0, 40, 80, 120, or 160 mM plus Hoagland's solution were applied to these pots for 150 days. The results showed that chlorophylls (a), (b), and (a+b) reduced with increasing of salinity up to 40 mM. There was no difference between cultivars for chlorophylls (b) and (a+b) while ‘Roghani’ showed more decreased in content of chlorophyll (a) than did ‘Zard’. Total soluble sugars in leaves increased with an increase in salinity up to 80 mM but decreased with additional increase in salinity. Salinity stress reduced water potential equally in both cultivars. The concentrations of sodium (Na) and chloride (Cl) and Na/potassium (K) ratio were increased and K decreased with increasing of salinity up to 80 mM in leaves and roots. Concentrations of K, magnesium (Mg), calcium (Ca), phosphorus (P), and nitrogen (N) reduced significantly in leaves, shoots and roots with increasing salinity.     

EFFECTS OF SALINITY AND CALCIUM ON THE GROWTH AND CHEMICAL COMPOSITION OF PISTACHIO SEEDLINGS

Poor quality of irrigation water (high salinity) has reduced the yields of pistachio over recent years, especially in Kerman. The effects of four salinity levels [0, 30, 60, and 90 mM sodium chloride (NaCl)] and three calcium (Ca) levels [0, 0.5, and 1 mM Ca as calcium nitrate (Ca(NO₃)₂.4H₂O)] on growth and chemical composition of pistachio seedlings cv. ‘Badami’ were studied in sand culture under greenhouse conditions in completely randomized design (CRD) with four replications. After 170 days, leaf area, leaf number, shoot and root dry weights were determined. Also shoot and root sodium (Na), potassium (K), Ca, and magnesium (Mg) concentrations were measured. Results showed salinity decreased all growth parameters. Ca application increased shoot and root Ca concentrations and root K concentration, while Ca application decreased shoot K concentration and shoot and root Mg concentrations. Salinity decreased shoot Ca, root K, and root Mg concentrations, while salinity increased shoot and root total sodium uptake, and shoot and root Cl concentrations.     

Effect of Salinity and Nitrogen Application on Growth,Chemical Composition and Some Biochemical Indices of Pistachio Seedlings (Pistacia Vera L.)

To study the effect of nitrogen and salinity on growth and chemical composition of pistachio seedlings (cv. ‘Badami’), a greenhouse experiment was conducted. Treatments consisted of four salinity levels [0, 800, 1600, and 2400 mg sodium chloride (NaCl) kg^?1 soil], and four nitrogen (N) levels (0, 60, 120, and 180 mg kg^?1 soil as urea). Treatments were arranged in a factorial manner in a completely randomized design with three replications. The highest level of nitrogen and salinity decreased leaf and root dry weights. Nitrogen application significantly increased the concentration of shoot N and salinity suppressed shoot N concentration. Salinity and nitrogen fertilization increased shoot and root sodium (Na), calcium (Ca), and magnesium (Mg) concentrations. Nitrogen application increased proline concentration and reducing sugar content. Although salinity levels increased proline concentration a specific trend on reducing sugars content was not observed.     

Ion compartmentation in salinity‐stressed alfalfa seedlings growing under different temperature regimes

Abstract

The compartmentation of ions in salinity‐stressed 6‐week‐old alfalfa seedlings was investigated in two cultivars, Batini (moderately salinity tolerant) and Hunter River (salinity sensitive). Both cultivars were grown under field conditions during the cool season (20–25°C) and the warm season (35–46°C). Two saline solutions (6.4 and 12.2 dS m^‐1) were prepared by dissolving the required quantity of NaCl in half‐strength Hoagland solution. Tap water (0.8 dS m^‐1) served as control. Plants were irrigated twice a week with saline solutions, commencing 3 weeks after seedling emergence. The seedlings were separated into leaf (including petiole), stem, and root after 6 weeks of growth and analysed for phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sodium (Na), chloride (Cl), and micronutrients [copper (Cu), boron (B), zinc (Zn), manganese (Mn), and iron (Fe)]. The concentration of P and K in the plant tissue increased with increase in temperature, while Ca and Mg concentrations were depressed. Increase in salinity, in general, resulted in decreased accumulation of all ions, including micronutrients. Hunter River maintained the same pattern of ion compartmentation (root<stem<leaf) for Na and Cl, whereas in Batini a different pattern was obtained for Na (stem<leaf<root). Since the compartmentation pattern of ions in Batini is different for Na and not Cl, it appears that compartmentation of Na may be an important factor in salt tolerance.     

COMPARISON OF YIELD,NUTRIENT SOLUTION CHANGES AND NUTRITIONAL STATUS OF GREENHOUSE TOMATO GROWN IN RECIRCULATING AND NON-RECIRCULATING NUTRIENT SOLUTION SYSTEMS

Studies were carried out in the years 2005–2007 with greenhouse tomato (Lycopersicon esculentum Mill.) cv. ‘Emotion F₁’ grown in rockwool with the recirculating and non-recirculating nutrient solution systems. No significant differences were found in yields of fruits in both systems. In the recirculating system there was more intensive accumulation of sodium (Na), calcium (Ca), chloride (Cl), and zinc (Zn) in the root environment. Leaves of tomato grown in the recirculating nutrient solution system showed a higher content of Ca, magnesium (Mg), and Zn. There were no differences in the contents of nitrates and nitrites in fruits. In the recirculating system, the following savings were recorded: 42.5% of water and (in%): 42.1 nitrogen (N)- ammonium (NH₄), 56.0 N- nitrate (NO₃), 31.4 phosphorus (P), 52.1 potassium (K), 63.5 Ca, 47.9 magnesium (Mg), 49.4 sulfur (S)- sulfate (SO₄), 51.9 Cl, 50.9 iron (Fe), 47.9 Zn, 24.6 manganese (Mn), 53.3 copper (Cu) and 47.2 boron (B). A high effectiveness in decreasing of bacteria number in drain water by UV irradiation was found.     

外源 GSH 对 NaCl 胁迫下番茄幼苗叶片及根系离子微域分布的影响

【目的】盐胁迫是限制新疆番茄生长的重要障碍因子之一,而外源喷施谷胱甘肽 (GSH) 是解决这一问题的有效措施。探讨外源 GSH 缓解番茄盐胁迫的效应和作用机制,可为该措施的有效应用提供理论依据。 【方法】采用营养液栽培法,选用番茄品种‘中蔬四号’为试材。在营养液中加入 NaCl 100 mg/L,使其产生盐胁迫,以不加 NaCl 作为对照 (CK),试验处理包括不喷施 GSH (NaCl)、喷施 GSH (+ GSH)、喷施 GSH 合成酶抑制剂 (+ BSO) 以及喷施 GSH 和 BSO (+ BSO + GSH)。测定番茄幼苗叶片和根系中与耐盐性相关的 K+、Ca2+、Mg2+、Na+ 和 Cl– 的离子微域分布状态和平衡。 【结果】NaCl 胁迫下番茄叶片和根系所有组织细胞中 Na+ 和 Cl– 相对含量显著提高,K+ 相对含量和 K+/Na+、Ca2+/Na+、K+/Cl– 比值降低,说明 NaCl 胁迫使细胞中 Na+ 和 Cl– 有害离子积累及胞内离子稳态严重破坏;外源 BSO 施用进一步加剧了 NaCl 胁迫下番茄叶片和根系细胞的 K+/Na+ 失衡。而外源 GSH 施用抑制了 NaCl 胁迫下番茄叶片和根系对 Na+ 的吸收,降低了 Cl– 的相对含量,提高了 K+/Na+、Ca2+/Na+、K+/Cl– 比值。外源 GSH 亦使 NaCl+BSO 胁迫下番茄叶片各组织及根系中皮层、内皮层和中柱的 Na+ 未检出,根系和叶片各组织中 Cl– 相对含量显著降低,K+ 和 Ca2+ 相对含量及 K+/Na+、Ca2+/Na+、K+/Cl–、Ca2+/Cl– 比值显著提高。 【结论】外源 GSH 通过抑制盐胁迫下番茄叶片和根系对 Na+ 的吸收,降低 Cl– 吸收,改善细胞中离子的微域分布和维持离子平衡, 从而缓解了盐胁迫对番茄的毒害作用,提高了番茄的耐盐性。     

Distribution of K, Na and Cl in Root and Leaf Cells of Soybean and Cucumber Plants Grown under Salinity Conditions

The element contents in the compartments of root and leaf cells of soybean and cucumber plants grown for 8 d in a nutrient solution containing 50 mM NaCl, 25 mM CaCl₂ or 50 mM NaCl+4.75 mM CaSO₄ were examined by X-ray microanalysis of freeze-substituted dry sections. Sodium accumulated in the vacuoles rather than in the cytoplasm and apoplastic space in the root cells of the soybean plants, leading to the difficulty in the transport of Na to leaves in soybean. Salt injury of soybean is considered to be caused by the accumulation of Cl at high concentrations in all the compartments of root and leaf cells. In contrast, the accumulation of Na in the cytoplasm of the root and leaf cells might disturb the metabolism and lead to the occurrence of salt toxicity in cucumber plants, which are tolerant to Cl due to the stimulation of Cl accumulation in vacuoles when the Ca concentration was high in nutrient media.     

Effects of gypsum and Zn on uptake ratios of Na,K and growth-yield of rice grown on a coastal saline soil

Salinity reduced (reduction below control = RBC) grain yield of rice by 80 and 98% at 8 and 16 mS/cm levels of saline irrigation, respectively, below tap water (0.6 mS/cm). The adverse effect of salinity was more pronounced on grain yield (98% RBC at 16 mS/cm) than on straw yield (84% RBC). The combined application of gypsum and Zn at the rates of 160 and 5 kg/ha produced 49, 45, and 41% more grain yield above controls at 0.6, 8, and 16 mS/cm levels of salinity, respectively. The length of panicles, percentage of filled grains, and 1000-grain weight, protein concentrations of the rice grains, sum of Ca, Mg and K concentrations were reduced due to imposition of saline irrigation, but gypsum and Zn treatments significantly improved these parameters even at the highest (16 mS/cm) salinity stress. The combined application of gypsum and Zn was found to be effective to increase the protein concentrations in rice grains by 2–4% and to increase total concentrations of Ca, Mg and K, but reduced the Na/K ratios in plant tissues by 29.13, and 12% at 0.6, 8, and 16 mS/cm salinity, respectively, suggesting that the application of gypsum and Zn in parallel with irrigation of saline soils, would be effective to reduce the adverse effects of high Na/K ratios as well as to improve growth, yield, and nutritional balance in rice.