Phosphate Fertilizers for Sugarcane Used at Pre-Planting (Phosphorus Fertilizer Application)

Phosphate applications are still rare in sugarcane cultivation and can be done with phosphorus fertilizers of residual over. This study aims to evaluate the agronomic efficiency of phosphate fertilization before sugarcane planting and its effect on sugarcane yield. The study was carried out over three crop cycles at the Jalles Machado sugar-mill in Goianésia, GO, Brazil. The treatments consisted of five different phosphorus sources (soluble and insoluble) applied at a rate of 300 kg ha^?1 phosphorus pentoxide (P₂O₅). The phosphorus fertilizers used were: triple superphosphate, mono-ammonium phosphate (MAP), Arad rock phosphate, Itafós rock phosphate, and magnesium term phosphate. The effects of these fertilizers were evaluated by plant height (ground to Top Visible Dewlap; TVD), leaf phosphorus content, agronomic efficiency, and sugarcane yield from cane-plants, first ratoon, and second ratoon. Magnesium term phosphate and triple superphosphate resulted in greater plant-cane height. Leaf phosphorus concentrations were highest with applications of MAP, magnesium term phosphate, and triple superphosphate. Phosphate applications did not affect sugarcane plant yield, but did increase ratoon yield. In general, the residual effect of insoluble phosphate increased over the years. The agronomic efficiency (AE) of phosphate was (in descending order): triple superphosphate (100%), term phosphate (89%), MAP (80%), Itafós rock phosphate (67%), and Arad rock phosphate (60%).     

Vergleichende Untersuchungen zur Pflanzenverfügbarkeit von Phosphat aus verschiedenen P‐Recycling‐Produkten im Keimpflanzenversuch

Die Weltreserven an Rohphosphaten hoher Qualität und moderater Abbaukosten reichen noch für ca. 90 Jahre. Daraus leitet sich die Notwendigkeit des Phosphat (P)‐Recyclings aus P‐haltigen Abfällen ab. In Deutschland fallen jährlich 2,4 × 10⁶ t Trockenmasse Klärschlamm und über 600.000 t Abfälle tierischen Ursprungs an. Mit dem daraus recycelten P könnte der größte Teil der bisherigen P‐Mineraldünger ersetzt werden. Verlässliche Daten über die Pflanzenverfügbarkeit des P liegen für viele Produkte des P‐Recyclings jedoch noch nicht vor. In einem 21‐tägigen Keimpflanzenversuch (Applikation von 20 mg P (300 g Quarzsand)^–1 in 10 cm × 10 cm × 6 cm großen Plastikgefäßen; Aussaat von 100 Roggenkörnern; Einstellung auf 65% der max. Wasserkapazität) wurden daher 26 P‐Verbindungen überprüft (u. a. verschiedene Calcium‐, Magnesium, Eisen‐, Aluminium‐, Ammonium‐ bzw. Magnesium‐Ammonium‐Phosphate, Thomasphosphate). Als Kontrolle wurde eine Variante ohne P‐Gabe eingesetzt. Die Netto‐P‐Aufnahme (P‐Menge in Sprossen und Wurzeln minus P‐Menge in den Kontrollpflanzen) ergab Auskunft über die P‐Verfügbarkeit für die Pflanzen. Die P‐Aufnahme aus den Ca‐Phosphaten sank erwartungsgemäß vom primären Ca‐Phosphat (als Referenz gleich 100 % gesetzt) bis zum Ca‐Apatit auf 10 %. Aus primärem, sekundärem und tertiärem Mg‐Phosphat wurden P‐Mengen ähnlich denen aus dem primären Ca‐Phosphat aufgenommen. Das gleiche galt für die P‐Aufnahme aus Ammonium‐Phosphat und Magnesium‐Ammonium‐Phosphat (Struvit). Thomasphosphate und Sinterphosphate zeigten nur etwa 42 bis 65 % der P‐Verfügbarkeit von primärem Ca‐Phosphat. Insbesondere Fe‐Phosphat versagte völlig als P‐Quelle. Als agronomisch interessante P‐Recyclingprodukte erwiesen sich die Magnesium‐ sowie die Magnesium‐Ammonium‐Phosphate. Zur weiteren Absicherung dieser Ergebnisse müssen diese Produkte in verschiedenen Böden sowohl in Gefäß‐ als auch Feldversuchen überprüft werden.     

Determination of trace amounts of phosphate in water extracts of soils

Abstract

Two methods are described for determining water‐soluble phosphate in the range 0.5 to 10 μg P/l, using solvent extraction for the concentration of molybdenum blue. The manual shaking method had a precision of 0.02 μg P/l; the continuous extraction method, a precision of 0.05 μg P/l. The methods have been found suitable for measuring phosphate in water extracts of soils.     

Mechanisms of increase in content of water-soluble P in Hachirogata reclaimed soils after NaCl washing

Abstract

In some of the drainage waters in Hachirogata reclaimed land, a very high concentration of inorganic P (up to 2 mg P L^-1) has been recently detected (Sato et al. unpublished). The drainage waters flow into the Lake Hachirogata that surrounds the land. Since the lake is also a reservoir, the water circulates through a closed system. Recently, the eutrophication of the lake has been found to be significant (Akita Prefectural Government 1989) and drainage water has been incriminated as one of the P sources of eutrophication. Therefore, it would be important to analyze the mechanisms responsible for the increase of P release from Hachirogata reclaimed land.     

磷矿粉在酸性土壤中的施用: 比较磷酸盐提取方法测定磷矿粉溶解程度

Three phosphate extraction methods were used to investigate the dissolution,availability and transfo-mation of Kunyang phosphate rock(KPR) in two surface acid soils.Dissolution was determined by measuring the increase in the amounts of soluble and adsorbed inorganic phosphate fractions,and did not differ signifi-cantly among the three methods.Significant correlations were obtained among P fractions got by the three extraction methods.Dissolution continued until the end of the 90-day incubation period.At the end of the period,much of the applied phosphate recovered in both soils were in the Al- and Fe-P or in the hydroxide-and bicarbonate-extractable inorganic P fractions.The dissolution of KPR in the two soils was also similar: increased addition of phosphate rock resulted in decreased dissolution.The similarity in the order and extent of dissolution in the two soils was probably due to the similarity in each soil of several factors that are known to influence phosphate rock dissolution,namely low CEC,pH,P level,and base status;and high clay and free iron and aluminum oxide contents.The results suggested that KPR could be an aternative P source in the soils are not limiting.     

Changes in net ecosystem production associated with forest fire in taiga ecosystems,near Yakutsk,Russia

Abstract

The effect of sucrose on the growth of rice plants under gnotobiotic conditions was studied at test tube scale in vinyl isolators under weak or strong light. Under weak light (500 lux) the growth of rice plants on agar medium containing only inorganic nutrients during the 20 days after transplanting stopped at 3rd leaf stage, whereas plants on agar medium containing inorganic nutrient plus 2% sucrose grew to 5th or 6th leaf stage. Under strong light (1.5 × 10⁴ lux) the stage of tillering was promoted by supplying sucrose. The growth of rice plants in pot-hydroculture under weak light had the same tendency as that obtained in test tube culture under weak light.

Considering the concentration of inorganic elements in rice shoots, it seems most likely that the uptake of inorganic elements. especially phosphorus and potassium, was enhanced by the supplements of 2% sucrose under weak light. An increase of calcium and manganese uptake was observed in plants grown in medium containing 2% sucrose compared with that of plants grown in inorganic medium alone under strong light. The uptake of inorganic elements from medium containing inorganic nutrients plus 2% sucrose depended chiefly on light energy under strong light in the phytotron used.     

解磷菌剂对作物生长和土壤磷素的影响

利用课题组自行分离筛选的B2和B67解磷菌株研制的菌剂，在盆栽和大田试验过程中，菌剂在增加作物产量的同时，有提高土壤速效磷含量、培肥土壤的作用，而且是菌剂和化肥配合施用效果显著。施用了解磷菌剂或菌剂和化肥配合施用后，盆栽玉米的株高、鲜重和干重，较CK增加15．2％～89．2％，玉米单株吸养量较CK增加20％～146％；青菜的鲜重、干重和生物量较CK增加15．8％～41．6％，和CK比较差异显著；小麦的株高、有效小穗数、穗粒数、粒重以及生物量也较CK差异显著，大田试验的几种作物也有不同程度的增产。另外B2和B67接种到山西省典型土壤中，土壤速效磷含量较CK增加1．35～3．04倍，且发现其溶磷效果和土壤磷酸酶活性与有效活菌数相关，解磷菌剂在提高土壤速效磷含量的同时，也有提高土壤速效钾含量的作用。     

棉田土壤上几种磷肥用量估算法的比较研究

本文通过田间试验和室内模拟试验研究了不同磷肥估算法在棉田土壤上的应用。结果表明: 肥效函数法、土壤吸附等温线法、土壤磷酸盐吸收系数法和磷指标法均能应用于棉田土壤,砂壤质棉田由此估算的施磷量分别为P2O5 148、173、168和150 kg/hm2,壤质棉田施磷量分别为P2O5 138、160、153和172 kg/hm2,其中以土壤磷酸盐吸收系数法操作最简便且精度与肥效函数法相当。     

Phosphorus Stress-Induced Differential Growth,and Phosphorus Acquisition and Use Efficiency by Spring Wheat Cultivars

ABSTRACT

Phosphorus (P) is a finite, non-renewable, and natural resource and a vital major nutrient for plant metabolic and developmental processes. However, adverse soil biogeochemical characteristics of alkaline-calcareous soils (especially Aridisols) and highly weathered acid soils (i.e., Ultisols and Oxisols) render orthophosphate (Pi) as the least available major nutrient due to P complexation, sorption, and/or fixation. In such soil environments, plant bioavailable P is only a small fraction of total soil P, seriously limiting crop growth and production. Different plant species, and even cultivars of the same species, may display a suite of growth responses that enable them to solubilize and scavenge soil P either by enhancing external Pi acquisition or reprioritizing internal Pi use under P-stress soil environments. This paper reports relative growth responses, P acquisition and P-use efficiency characteristics by 14 cultivars of spring wheat (Triticum aestivum L.) grown in solution culture with high/low P supply induced by applying soluble NH₄H₂PO₄, sparingly soluble rock phosphate, and Ca₃(PO₄)₂. The wheat cultivars exhibited considerable genetic diversity in biomass accumulation, P concentrations, P contents, factor (PSF) and P efficiency characteristics [i.e., P utilization efficiency (PUE), P efficiency (PE), and PE ratio (PER)]. Plant growth and PE parameters were significantly correlated, while P uptake was linearly related with biomass increase and solution pH decrease. The wheat cultivars with high PUE, PER and P uptake, and low PSF, and plant P concentration were more efficient in utilizing P and, hence, more tolerant under P-stress environment. Biomass and P contents of “P efficient/low-P tolerant” wheat cultivars were superior to “P inefficient/low-P sensitive” cultivars at all P-stress levels. Hence, “P efficient/low-P tolerant” cultivars are the most desirable wheat genotypes for P-stress environments because they are able to scavenge more P from sparingly soluble P sources or soil-bound P forms.     

Early detection of phosphate deficiency in plants

Abstract

At 3, 7 and 10 days after planting, uptake of ³²P‐ orthophosphate over 15 minutes by pine seedlings grown in phosphate deficient soil was 3.0 4.8 and 5.2 times that of seedlings growing in phosphate amended soil. At 4 days and 7 days uptake over 15 minutes by phosphate deficient wheat was 1.2 and 2.4 times that of plants grown in phosphate amended soil. A rapid, sensitive, very early bioassay of available soil phosphate is suggested.     

Phosphordüngewirkung eisenhaltiger Klärschlämme

<?show $38#Bo;>Phosphorus fertilization effect of sewage sludges treated with iron compounds The result of waste water treatment with iron salts are sewage sludges with higher P but also Fe contents. The effect of such sludges on P availability in soils is not clear and was, therefore, compared with CaHPO₄ as a P mineral fertilizer. In experiments (Mitscherlich pots, 6 kg soil) two sandy soils (pH 4.3 and 4.9), two luvisols ( pH 6.3 and 7.2), a loess (13 % CaCO₃), and quartz sand all differing in their P status were treated with two sewage sludges which differed in their molar Fe:P ratios (sludge Gö: 1:0.3, sludge Sh: 1:1.2). For sludge Gö the P elimination had been carried out with FeSO₄ and the sludge was stabilized with FeCl₃ + Ca(OH)₂ (filter press). For sludge Sh P was eliminated by FeCl₃ in the aerobic basin. The first crop was maize (total shoot dry matter), the second red clover. In case of sludge Sh both crops took up in comparison to CaHPO₄ the same amount of P from all substrates with the exception of quartz sand. The effect of sludge Gö was quite different: from the neutral luvisol soils maize was able to take up only 64—82 % P and red clover 77—82 % P only as compared to CaHPO₄. On the sandy soils maize and red clover grew after sludge Gö as good or better than after CaHPO₄ application and P uptake was quite similar (83—106 %). This result was concomitant to an increase of pH values (from 4.3 to 4.9, 4.9 to 5.6, respectively). The negative efficiency of P uptake in heavy soils after application of sludge Gö resulted from an increase of P sorption and decrease of orthophosphate concentration in soil solution. This sludge contains a high proportion of iron hydroxides/oxides with free sorption sites for P. In future P elimination from waste water should be done without iron salts. At least during the process of conditioning no Fe salts should be used.<?show $6#>     

几株高效溶磷菌株对不同磷源溶磷活力的比较

在液体培养条件下，研究了4株溶磷菌株(Bmp5、Bmp6、Bmp7和Fmp9)对不同磷源溶解能力的差异并与荧光假单孢菌As1.867和巨大芽孢杆菌As1.223进行了比较，探讨了菌株组合培养对溶磷活力的影响。结果表明，4株菌株对磷酸钙、磷酸铝、磷酸氢钙溶解能力明显高于磷酸铁和卵磷脂。以磷酸钙为磷源时，Fmp9的溶磷量比As1.867和As1.223分别高出约92%和48%；而以磷酸铝为磷源时，As1.223的溶磷量明显高于其他菌株；在磷酸氢钙为磷源的条件下，Bmp6为优势菌株，溶磷量高达785.51mg/L。对比研究发现，Bmp5、Bmp6、Bmp7及Fmp9的优势磷源分别为卵磷脂、磷酸氢钙、磷酸铝和磷酸钙。组合培养表明，Bmp5+Fmp9和Bmp6+Fmp9较单株菌的溶磷量有所增加，为较好的组合。试验得到的溶磷微生物配方已经应用于生物复合肥料的研究，并进行了盆栽实验，得到了较好的效果。该研究可为土壤生物肥料工业的微生物学研究提供借鉴。     

磷肥施用对水稻生长和磷素吸收的影响

采用田间微区试验研究不同磷肥的施用位置和类型对姜堰、广德两地水稻生长、磷素吸收以及施肥后地表水磷浓度的影响,为水稻根区施磷提供科学依据。磷肥种类为磷酸二氢钙(MCP)和磷酸氢二铵(DAP),施用方式有:1表面撒施;2偏3 cm,深5 cm穴施;3偏12 cm,深5 cm穴施。结果显示施磷对两地水稻均无显著增产,姜堰地区水稻产量显著高于广德。不同施磷位置对水稻吸磷量有显著影响,姜堰、广德两地MCP偏3 cm处理的水稻总吸磷量较MCP偏12 cm处理分别高13%和12%,且DAP偏12 cm处理的水稻总吸磷量显著高于MCP偏12 cm处理,两地增幅分别达到12%和16%,磷肥撒施虽然在水稻分蘖期有较高的吸磷量,但在水稻成熟期不如磷肥偏3 cm穴施。施肥第10天的地表水总磷浓度以磷肥表面撒施处理较高,比磷肥穴施处理分别高245%(姜堰)和94%(广德)。以上结果表明:1适当位置的磷肥穴施不但能够促进水稻对磷的吸收,还能有效缓解径流排水造成的磷流失;2磷酸氢二铵的磷肥适宜施用距离范围较磷酸二氢钙远。     

厌氧条件总磷还原为磷化氢种泥筛选及功能菌株鉴定

厌氧除磷是一种高效、节能、低耗的方法。获取经济、方便、高效的种泥是实现该方法的前提,种泥中产生磷化氢功能菌株的组成及特性是提高处理效能和开发新工艺的基础,目前此研究未见有关报道。本研究依据厌氧除磷理论,利用厌氧培养反应瓶、筛选培养基和微生物筛选、分离、鉴定的方法。以A2/O厌氧池、污泥浓缩池污泥、养殖场新鲜猪粪、鸡粪、牛粪及鸭粪为研究种泥,通过跟踪厌氧培养过程培养液中总磷的去除率和吸收液中磷化氢的生成量筛选出最佳种泥,并对最佳种泥的菌株进行筛选、分离和鉴定。结果表明,6种泥中鸡粪的厌氧除磷能力最强,鸭粪能力最弱,浓缩池中的污泥和猪粪次之,牛粪及厌氧池污泥有一定的作用。本试验条件下鸡粪是厌氧除磷的最佳种泥。经过3个周期的厌氧培养,6种泥培养液总磷的去除率和吸收液中磷化氢的含量随培养时间增长都有不同程度增加。鸡粪的最佳培养时间为5d。鸡粪培养液经21d培养后分离到1株芽孢杆菌属、1株假单胞菌属及2株肠杆菌科,明确了2株肠杆菌科中1株为埃希氏菌属另1株为柠檬酸杆菌属。     

不同形态磷肥的径流流失特征及其效应

采用野外径流小区方法研究不同形态磷肥的流失特征、流失量以及对氮钾元素的影响。结果表明,在磷的流失途径中,颗粒态磷(PP)占总磷(TP)流失量的61.6%～83.1%,是磷流失的主要途径;施用磷肥可以促进土壤对氮钾元素的保持能力,减少氮钾元素的流失;磷肥的施用提高了小油菜的产量,并促进了小油菜对氮钾元素的吸收;微晶化磷矿粉可以有效减少磷的流失,在等磷量下,微晶化磷矿粉中的磷流失量分别是过磷酸钙和钙镁磷肥的54.2%和69.4%。在一定范围内,随微晶化磷矿粉施用量的增加,磷的流失量增加,但流失率减小,对促进土壤保持氮钾能力及小油菜对氮磷钾元素的吸收越明显。     

Exchangeability of phosphate extracted by four chemical methods

Isotopically exchangeable phosphate (P) is a major source of P for plants. In practice, however, plant‐available P is assessed by chemical extractions solubilizing a mixture of P forms the availability of which is ill defined. We undertook an isotopic approach to assess the exchangeability of P extracted by (1) CO₂‐saturated water (P‐CO₂), (2) ammonium acetate EDTA (P‐AAEDTA), and (3) sodium bicarbonate (P‐NaHCO₃) compared to the exchangeability of P extracted by water. Five topsoils with similar P‐fertilization histories but different soil properties were studied. Phosphorus was extracted from soils labeled with carrier‐free ³³P after 1 week of incubation, and the specific activity (SA = ³³P / ³¹P) of the extracts was compared with the SA of P extracted by water to calculate the amount of P isotopically exchangeable that had been solubilized during the extraction. P‐CO₂ extracted between 20 and 100 times less P than P‐AAEDTA and P‐NaHCO₃. The SA of P‐CO₂ was not different from the SA of water‐extractable P, showing that P‐CO₂ solubilized similar forms of P as water and that these forms can be considered as available. The SA of P extracted by the two other methods ranged between 25% and 63% for P‐AAEDTA and 66% and 92% for P‐NaHCO₃ of the SA of water‐extractable P. The fraction of exchangeable P extracted by AAEDTA decreased linearly with increasing soil pH, suggesting that this method dissolves slowly or non‐exchangeable P from calcium phosphates.     

Investigation on Phosphate Solubilization Potential of Agricultural Soil Bacteria as Affected by Different Phosphorus Sources,Temperature, Salt,and pH

Ten phosphate-solubilizing bacterial strains belonging to genera Pseudomonas, Burkholderia, Enterobacter, Serratia, Klebsiella, and Aeromonas were tested for mineral phosphate solubilization activity in Pikovskaya's broth using different phosphate sources at four temperatures (15, 25, 35, and 45 ^οC). Dicalcium and tricalcium phosphate were solubilized more effectively (≥1000 mg L^?1) than ferric and rock phosphate (≥100 mg L^?1) and 35 °C was found to be the optimum temperature. Although Klebsiella and Aeromonas spp. are well known for their dinitrogen (N₂)–fixing ability, to the best of our knowledge, this is the first report of inorganic phosphate solubilization by Klebsiella terrigena and Aeromonas vaga. Interestingly, A. vaga BAM-77 is the most efficient strain at solubilizing inorganic phosphorus (P) even in the presence of 8% sodium chloride (NaCl) at pH 10. These findings indicate that all four strains are efficient P solubilizers under variable conditions of temperature, pH, and P source and thus can be recommended for P fertilization in different soils.     

Phosphorus characterization of manure composts and combined organic fertilizers by a sequential‐fractionation method

Characterization of the forms of phosphorus (P) in organic soil amendments was conducted by sequential P fractionation. More than 60% of total P was inorganic P (P_i). The major P_i forms in the cattle‐manure composts were NaHCO₃‐ and HCl‐extractable P fractions. HCl‐extractable P_i was the predominant P form and a considerable proportion of the total P was present in the HCl‐extractable organic P fraction in the poultry manure composts and combined organic fertilizers.     

Phosphorus Sources and Rates Associated with Nitrogen Fertilization in Mombasa Grass Yield

Nitrogen (N) and phosphorus (P) deficiency is one of the important causes of degradation of cultivated pasture under tropical conditions. The aim of this study was to evaluate phosphate rates and sources, and N rates on the concentration and uptake of N and P, and shoot dry mass (SDM) yield of Megathyrsus maximum grass cv Mombasa in an Ultisol. The trial was carried out in a greenhouse in pots with 4.0 dm^?3 of soil. The experiment was arranged in a completely randomized design with four replicates. The 3 × 3 × 3 factorial treatments consisted of phosphorus sources [reactive rock phosphate from Morocco (RPM), reactive rock phosphate from Algeria (RPA) and triple superphosphate (TSP)], three phosphorus rates (0, 150, and 300 mg kg^?1), and three N rates (0, 250, and 500 mg kg^?1). The SDM and tillering of Mombasa grass were significantly influenced with the TSP, RPM, and RPA application associated with N fertilization. The RPM, RPA, and TSP met the nutritional demands of Mombasa grass. The three P sources showed the same effect on the total N uptake by Mombasa grass. The P use efficiency (PUE) when fertilizer-P sources were added alone by Mombasa grass was <12% of the added P, and PUE decreased as follows: TSP > RPA > RPM. When P and N-fertilizer were added together, the fertilizer-N use efficiency (NUE) was 62%. The reactive phosphate (RPM and RPA) is an efficient P sources for Mombasa grass, but requiring higher rate of application compared to TSP source.     

Phosphate fertilizer form and application strategy affect phosphorus mobility and transformation in a drip‐irrigated calcareous soil

The objective was to increase understanding about how P fertilizer form and application strategy affect P mobility and use efficiency in a drip‐irrigated calcareous soil. The study used two P forms (granular or liquid P) and two application methods (single or repeated application). Phosphorus uptake by roots was simulated by using diffusive gradients in thin films (DGT). Six fractions of inorganic P were obtained using the Jiang‐Gu sequential fractionation scheme. Mobility of P was greater when the fertilizer was applied in liquid rather than granular form. Depending on the fertilizer application rate, Olsen P concentrations 13.5–43 mm from the application point were larger by 22–96% in the liquid P treatment than in the granular P treatment. Liquid P increased DGT‐absorbed P by 29% and Ca₂‐P by 17% compared with granular P averagely. The P uptake and PUE were averagely larger by 32 and 121%, respectively, in the liquid P treatment than in the granular P treatment. In conclusion, liquid P fertilization reduces P fixation and increases P diffusion, availability, and bioavailability in calcareous soil. Phosphorus mobility is greater when the fertilizer is applied in a single rather than repeated application.