红树林恢复过程中土壤有机碳的变化

Based on total carbon (C) and C isotopes in sediment cores, sedimentary organic carbon (SOC) was quantified in three types of mangrove sites (barren flat sites without mangroves, mangrove plantations, and natural mangrove forests), which were considered to represent a continuum from least restored to most restored sites in southern China. SOC densities in the barren sites, plantations, and natural forests were 90, 170 and 288 Mg ha-1, respectively. We inferred that mangrove restoration increased SOC accumulation in coastal areas. At 0--70 cm depth, SOC ?13C values in both mangrove sites ranged from -27.37‰ to -23.07‰, and exhibited gradual enrichment with depth. In contrast, the values in the barren flat sites remained around -22.19‰ and fluctuated slightly with depth. At 0--60 cm, the 14C ages of the SOC in the barren flat site, the natural mangrove site, and the artificial mangrove site ranged from 1 397 to 2 608, 255 to 2 453, and 391 to 2 512 years BP, respectively. In both types of mangrove sites but not in the barren flat sites, the enrichment of ?13C with depth was related to increases in SOC decay and SOC age with depth. According to analysis of 14C age, much of the mangrove-derived C was transported and stored at 0--60 cm depth under anaerobic conditions in both mangrove sites. The sediments of mangrove forests in southern China sequester large quantities of SOC during mangrove restoration.     

基于Century模型和1:500 000土壤数据库的华东旱地土壤有机碳动态模拟

Changes in soil organic carbon (SOC) in agricultural soils influence soil quality and greenhouse gas concentrations in the atmosphere. Dry farmland covers more than 70% of the whole cropland area in China and plays an important role in mitigating carbon dioxide (CO2) emissions. In this study, 4109 dry farmland soil polygons were extracted using spatial overlay analysis of the soil layer (1:500000) and the land use layer (1:500000) to support Century model simulations of SOC dynamics for dry farmland in Anhui Province, East China from 1980 to 2008. Considering two field-validation sites, the Century model performed relatively well in modeling SOC dynamics for dry farmland in the province. The simulated results showed that the area-weighted mean soil organic carbon density (SOCD) of dry farmland increased from 18.77 Mg C ha1 in 1980 to 23.99 Mg C ha1 in 2008 with an average sequestration rate of 0.18 Mg C ha1 year?1. Approximately 94.9% of the total dry farmland area sequestered carbon while 5.1% had carbon lost. Over the past 29 years, the net SOC gain in dry farmland soils of the province was 19.37 Tg, with an average sequestration rate of 0.67 Tg C year1. Augmentation of SOC was primarily due to increased consumption of nitrogen fertilizer and farmyard manure. Moreover, SOC dynamics were highly differentiated among dry farmland soil groups. The integration of the Century model with a fine-scale soil database approach could be conveniently utilized as a tool for the accurate simulation of SOC dynamics at the regional scale.     

华北平原夏玉米临界氮稀释曲线的验证

The concept of critical N concentration （Nc） has been widely used in agronomy as the basis for diagnosis of crop N status, and allows discrimination between field situations of sub-optimal and supra-optimal N supply. A critical N dilution curve of Nc= 34.0W-0.37, where W is the aboveground biomass （Mg DM ha-1） and Nc the critical N concentration in aboveground dry matter （g kg-1 DM）, was developed for spring maize in Europe. Our objectives were to validate whether this European critical N dilution curve was appropriate for summer maize production in the North China Plain （NCP） and to develop a critical N dilution curve especially for summer maize production in this region. In total 231 data points from 16 experiments were used to test the European critical N dilution curve. These observations showed that the European critical N dilution curve was unsuitable for summer maize in the NCP, especially at the early growth stage. From the data obtained, a critical N dilution curve for summer maize in the NCP was described by the equation of Nc = 27.2W-0.27, when aboveground biomass was between 0.64 and 11.17 Mg DM ha-1. Based on this curve, more than 90% of the data for the N deficiency supply treatments had an N nutrition index （NNI） 〈 1 and 92% of the data for the N excess supply treatments had an NNI 〉 1.     

一株敌敌畏降解菌DDV-1(Ochrobactrium sp.)的分离鉴定及其降解特性的研究

Soil organic carbon （C） and total nitrogen （N） pools of a Chinese fir （Cunninghamia lanceolata （Lamb.） Hook.） （CF） forest, and an evergreen broadleaf （EB） forest located in mid-subtropical, southeastern China, were compared before clearcutting, with the effect of slash burning on organic C and total N in the top 10 cm of soil before and after burning also being evaluated. Prior to clearcutting CF forest had significantly lower （P 〈0.05） organic C and total N in the soil （0-100 cm） compared to EB forest with approximately 60% of the C and N at the two forest sites stored at the 0 to 40 cm soil. In post-burn samples of the 0-10 cm depth at 5 days, 1 year, and 5 years for CF and EB forests, significantly lower levels （P 〈0.05） of organic C and total N than those in the pre-burn samples were observed. Compared to the pre-burn levels, at post-burn year 5, surface soil organic C storage was only 85% in CF forest and 72% in EB forest, while total N storage was 77% for CF forest and 73% for EB forest. Slash burning caused marked long-term changes in surface soil C and N in the two forest types.     

子午岭植被自然恢复过程中土壤有机碳密度的时空变化

To probe the processes and mechanisms of soil organic carbon （SOC） changes during forest recovery, a 150-year chronosequence study on SOC was conducted for various vegetation succession stages at the Ziwuling area, in the central part of the Loess Plateau, China. Results showed that during the 150 years of local vegetation rehabilitation SOC increased significantly （P 〈 0.05） over time in the initial period of 55-59 years, but slightly decreased afterwards. Average SOC densities for the 0-100 cm layer of farmland, grassland, shrubland and forest were 4.46, 5.05, 9.95, and 7.49 kg C m^-3, respectively. The decrease in SOC from 60 to 150 years of abandonment implied that the soil carbon pool was a sink for CO2 before the shrubland stage and became a source in the later period. This change resulted from the spatially varied composition and structure of the vegetation. Vegetation recovery had a maximum effect on the surface （0-20 cm） SOC pool. It was concluded that vegetation recovery on the Loess Plateau could result in significantly increased sequestration of atmospheric CO2 in soil and vegetation, which was ecologically important for mitigating the increase of atmospheric concentration of CO2 and for ameliorating the local eco-environment.     

中亚热带杉木林和常绿阔叶林土壤C、N库及其火烧后的变化

Soil organic carbon (C) and total nitrogen (N) pools of a Chinese fir (Cunninghamia lanceolate, (Lamb.) Hook.) (CF) forest, and an evergreen broadleaf (EB) forest located in mid-subtropical, southeastern China, were compared before clearcutting, with the effect of slash burning on organic C and total N in the top 10 cm of soil before and after burning also being evaluated. Prior to clearcutting CF forest had significantly lower (P < 0.05) organic C and total N in the soil (0-100 cm) compared to EB forest with approximately 60% of the C and N at the two forest sites stored at the 0 to 40 cm soil. In post-burn samples of the 0-10 cm depth at 5 days, 1 year, and 5 years for CF and EB forests, significantly lower levels (P < 0.05) of organic C and total N than those in the pre-burn samples were observed. Compared to the pre-burn levels, at post-burn year 5, surface soil organic C storage was only 85% in CF forest and 72% in EB forest, while total N storage was 77% for CF forest and 73% for EB forest. Slash burning caused marked long-term changes in surface soil C and N in the two forest types.     

基于进化遗传优化的自适应神经模糊推理新系统与地理信息系统的非洲山地土壤有机碳储量估算和绘图

Soil organic carbon (SOC) pool has the potential to mitigate or enhance climate change by either acting as a sink,or a source of atmospheric carbon dioxide (CO2) and also plays a fundamental role in the health and proper functioning of soils to sustain life on Earth.As such,the objective of this study was to investigate the applicability of a novel evolutionary genetic optimization-based adaptive neuro-fuzzy inference system (ANFIS-EG) in predicting and mapping the spatial patterns of SOC stocks in the Eastern Mau Forest Reserve,Kenya.Field measurements and auxiliary data reflecting the soil-forming factors were used to design an ANFIS-EG model,which was then implemented to predict and map the areal differentiation of SOC stocks in the Eastern Mau Forest Reserve.This was achieved with a reasonable level of uncertainty (i.e.,root mean square error of 15.07 Mg C ha-1),hence demonstrating the applicability of the ANFIS-EG in SOC mapping studies.There is potential for improving the model performance,as indicated by the current ratio of performance to deviation (1.6).The mnapping also revealed marginally higher SOC stocks in the forested ecosystems (i.e.,an average of 109.78 Mg C ha-1) than in the agro-ecosystems (i.e.,an average of 95.9 Mg C ha-1).     

基于土壤系统分类的中国土壤有机碳库分析

Changes in soil organic carbon (SOC) of rice paddies in China were simulated from 1980 to 2000 by linking a coupled bio-physical model to GIS database. The coupled model consists of two sub-models including Crop-C for simulating net primary productivity and hence residue retention and Soil-C for computing the turnover rates of SOC. The GIS database included parameters of climate, soils and agricultural activities with the resolution of 10 km × 10 km. Model simulation indicated that Chinese rice paddies covering 22.6 Mha sequestrated a considerable amount of C, about 0.15 ± 0.07 Pg C from 1980 to 2000. Annual sequestration rate increased sharply from -180 ± 45 kg C ha-1 year-1 in 1980 to 440 ± 170kg C ha-1 year-1 in 1989. Thereafter, a steady sequestration rate of 460 ± 170 kg C ha-1 year-1 occurred till 1994 and declined since then. Approximately 84% of the Chinese rice paddies sequestrated carbon, while 15% lost carbon and 1%kept balance over the 20 years. Great SOC sequestration occurred in eastern, southern and central China, while a slight decline of SOC existed in some regions of northeastern and southwestern China.     

Changes in profile distribution and chemical properties of natural nanoparticles in paddy soils as affected by long-term rice cultivation

Systematic studies on the genesis, properties, and distribution of natural nanoparticles(NNPs) in soil remain scarce. This study examined a soil chronosequence of continuous paddy field land use for periods ranging from 0 to 1 000 years to determine how NNPs in soil changed at the early stages of soil genesis in eastern China. Soil samples were collected from coastal reclaimed paddy fields that were cultivated for 0, 50, 100, 300, 700, and 1 000 years.Natural nanoparticles were isolated and characterized along with bulk soil samples( 2-mm fraction) for selected physical and chemical properties. The NNP content increased with increasing soil cultivation age at 60 g m-2 year-1, which was related to decreasing soil electrical conductivity(172–1 297 μS cm-1) and NNP zeta potentials(from-22 to-36 m V) with increasing soil cultivation age. Changes in several NNP properties, such as pedogenic iron oxide and total organic carbon contents, were consistent with those of the bulk soils across the soil chronosequence. Notably, changes in NNP iron oxide content were obvious and illustrated active chemical weathering, pedogenesis, and potential impacts on the microbial community. Redundancy analysis demonstrated that the soil cultivation age was the most important factor affecting NNP properties, contributing 60.7% of the total variation. Cluster and principal component analysis(PCA) revealed splitting of NNP samples into age groups of 50–300 and 700–1 000 years, indicating rapid evolution of NNP properties, after an initial period of desalinization(approximately 50 years). Overall, this study provides new insights into NNP evolution in soil during pedogenesis and predicting their influences on agriculture and ecological risks over millennial-scale rice cultivation.     

提高高产玉米氮素利用效率的根层氮素管理技术

Many recently developed N management strategies have been extremely successful in improving N use efficiency.How-ever,attempts to further increase grain yields have had limited success.Field experiments were conducted in 2007 and 2008 at four sites to evaluate the effect of an in-season root-zone N management strategy on maize (Zea mays L.).According to the in-season root-zone N management,the optimal N rate (ONR) was determined by subtracting measured soil mineral N (NH + 4--N and NO 3--N) in the root zone from N target values.Other treatments included a control without N fertilization,70% of ONR,130% of ONR,and recommended N rate (RNR) by agronomists in China that have been shown to approach maize yield potentials.Although apparent N recovery for the ONR treatment was significantly higher than that under RNR in 2007,grain yield declined from 13.3 to 11.0 Mg ha 1 because of an underestimation of N uptake.In 2008,N target values were adjusted to match crop uptake,and N fertilization rates were reduced from 450 kg N ha-1 for RNR to 225 to 265 kg N ha-1 for ONR.High maize yields were maintained at 12.6 to 13.5 Mg ha 1,which were twice the yield from typical farmers’ practice.As a result,apparent N recovery increased from 29% to 66%,and estimated N losses decreased significantly for the ONR treatment compared to the RNR treatment.In conclusion,the in-season root-zone N management approach was able to achieve high yields,high NUE and low N losses.     

Organic Carbon Dynamics in Different Soil Types After Conversion of Forest to Agriculture

State‐of‐the‐art predictive models of soil organic carbon (SOC) dynamics associated with land use changes are unable to reflect the diversity of tropical soil types as the knowledge of contrasting site‐specific factors in mediating the response of the SOC pool is sparse. This paper examines the influence of soil type and management on SOC dynamics following the conversion of forests to annual cropping in Ghana. Soil from primary forests and from areas with short (2–7 years) and long (20 years) histories of maize cultivation was sampled from a Vertisol dominated by smectite and Ultisol dominated by kaolinite. Wet sieving was used to separate soil fractions below and above 250 µm. SOC concentrations and δ¹³C signatures of SOC in soil fractions and bulk soil were determined. SOC stocks were calculated by the commonly used fixed depth approach and by the equivalent soil mass approach. After 20 years of cultivation of the Vertisol, the total SOC content was 40 per cent lower than under forest, and about 95 per cent of the forest‐derived SOC had been lost. After 20 years of cultivation of the Ultisol, total SOC content was only about 20 per cent lower than under forest and merely 30 per cent of the forest‐derived SOC had been lost. Both soil types were managed as they would typically be in small scale farming systems, thus the higher SOC losses and the substantial loss of forest‐derived SOC from the Vertisol question the conventional concept of smectite having a higher SOC‐stabilizing potential than kaolinite under field conditions. Copyright © 2013 John Wiley & Sons, Ltd.     

Soil Organic Carbon and Nitrogen Fractions under Different Land Uses and Tillage Practices

Many questions have surfaced regarding long-term impacts of land-use and cultivation system on soil carbon (C) sequestration. The experiment was conducted at Ohio Agricultural Research and Development Center. Only minor variations of soil organic carbon (SOC) and nitrogen (N) fractions with depth under plow tillage (PT). The SOC, total nitrogen (TN), microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) concentrations were higher under grassland and forestland in the top 0–15 cm depth than arable soils. No-tillage (NT) also increased SOC and N fractions concentrations in the surface soils than PT. Compared to arable, grass and forest could significantly improve proportions of MBC and MBN, and reduce proportions of dissolved organic carbon (DOC) and dissolved organic nitrogen (DON). NT and forest also increased the ratio of SOC/TN, MBC/MBN, and DOC/DON. Overall, grass and forest provided more labile C and improved C sequestration than arable. So did NT under arable land-use.     

Soil organic matter dynamics in lands continuously cultivated with maize and soybean in Heilongjiang Province,Northeast China: Estimation from natural 13C abundance

Abstract

Northeast China is the main production area of maize and soybean in China. In the present study, the rates of decomposition and replacement of soil organic carbon (SOC) were estimated using the soil inventory collected since 1991 from long-term maize and soybean cultivation plots in Heilongjiang Province, Northeast China, to evaluate the sustainability of the present cultivation system. The total carbon (C) content in soil was stable without any significant changes in the plots (approximately 28.5 g C kg^?1). The δ¹³C value of soil organic matter under continuous maize cultivation increased linearly with an annual increment of 0.07 from ?23.9 in 1991, which indicated that approximately 13% of the initial SOC was decomposed during the 13-year period of maize cultivation, with a half-life of 65 years. Slow decomposition of SOC was considered to result from the low annual mean temperature (1.5°C) and long freezing period (170–180 days year^?1) in the study area. In contrast, the amount of organic C derived from maize increased in the soil with a very slow annual increment of 0.17 g C kg^?1, probably because of the removal of all the plant residues from the plots. Based on the soil organic matter dynamics observed in the study plots, intentional recycling/maintenance of plant residues was proposed as a way of increasing soil fertility in maize or soybean cultivation.     

Carbon dynamics determined by natural C abundance in microcosm experiments with soils from long-term maize and rye monocultures

Understanding carbon dynamics in soil is the key to managing soil organic matter. Our objective was to quantify the carbon dynamics in microcosm experiments with soils from long-term rye and maize monocultures using natural ¹³C abundance. Microcosms with undisturbed soil columns from the surface soil (0-25 cm) and subsoil (25-50 cm) of plots cultivated with rye (C₃-plant) since 1878 and maize (C₄-plant) since 1961 with and without NPK fertilization from the long-term experiment ‘Ewiger Roggen’ in Halle, Germany, were incubated for 230 days at 8 °C and irrigated with 2 mm 10⁻² M CaCl₂ per day. Younger, C₄-derived and older, C₃-derived percentages of soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass (C_mic) and CO₂ from heterothropic respiration were determined by natural ¹³C abundance. The percentage of maize-derived carbon was highest in CO₂ (42-79%), followed by C_mic (23-46%), DOC (5-30%) and SOC (5-14%) in the surface soils and subsoils of the maize plots. The percentage of maize-derived C was higher for the NPK plot than for the unfertilized plot and higher for the surface soils than for the subsoils. Specific production rates of DOC, CO₂-C and C_mic from the maize-derived SOC were 0.06-0.08% for DOC, 1.6-2.6% for CO₂-C and 1.9-2.7% for C_mic, respectively, and specific production rates from rye-derived SOC of the continuous maize plot were 0.03-0.05% for DOC, 0.1-0.2% for CO₂-C and 0.3-0.5% for C_mic. NPK fertilization did not affect the specific production rates. Strong correlations were found between C₄-derived C_mic and C₄-derived SOC, DOC and CO₂-C (r≥0.90), whereas the relationship between C₃-derived C_mic and C₃-derived SOC, DOC and CO₂-C was not as pronounced (r≤0.67). The results stress the different importance of former (older than 40 years) and recent (younger than 40 years) litter C inputs for the formation of different C pools in the soil.     

Soil organic carbon in some land uses of Costa Rica

The native vegetation in the Tropics is increasingly replaced by crops, pastures, tree plantations, or settlements with contradictory effects on soil organic carbon (SOC). Therefore, the general objective was to estimate the SOC stock depth distribution to 100-cm depth in soils of Costa Rica and to assess their theoretical carbon (C) sink capacity by different management practices. A study was established in three ecoregions of Costa Rica: the Isthmian-Atlantic Moist Forest (AM), the Pacific Dry Forest (PD), and the Montane Forest (MO) ecoregions. Within each ecoregion, three agricultural land uses and a mature forest were sampled to 100-cm depth. The SOC stock in 0–100 cm depth was 114–150 Mg C ha^?1 for AM, 76–165 Mg C ha^?1 for PD, and 166–246 Mg C ha^?1 for MO. Land use had only weak effects on SOC concentrations and stocks except at PD where both were lower for soils under mango (Mangifera indica) and pasture. This may indicate soil degradation which was also supported by data on SOC stratification. However, it was generally unclear whether differences among land uses within each ecoregion already existed particularly at deeper depths before land-use change, and whether the sampling approach was sufficient to investigate them. Nevertheless, about 26–71% of Costa Rica's total C emissions may be offset by SOC sequestration in agricultural and forest soils. However, ecoregion-specific practices must be implemented to realize this potential.     

Effect of Land-Use History on Soil Carbon and Nitrogen in a Mediterranean Catchment

The impact of land use on soil organic matter was investigated. Five land cover types (pine forest, olive groves, wheat, wheat/maize cultivation systems, and a shrub pasture) belonging to three land-use categories from the same catchment in the island of Lesvos, Greece, were used. The soils developed under similar pedogenetic processes and accepted similar agricultural practices for at least 30 years. The results showed that the land-use and cover types ranged according to their total soil carbon (C) content as follows: forest > double cultivation > wheat > olive > pasture. Crop plantations contained 31 to 40% less C at their upper 0- to 45-cm layer than forest. Pasture had shallow soils with a small C accumulation but high C concentration, whereas olive groves had the lowest concentrations of both soil C and nitrogen (N). Olive grove soils were the most prone to degradation but possessed the greatest potential for C sequestration.     

内蒙农牧交错带地区土地利用方式和施肥对土壤碳库的影响

内蒙武川是我国典型的内蒙农牧交错带地区,土地利用方式转变和施肥是影响该地区农业生产和土壤碳储量的重要人类活动。选取内蒙武川地区,针对不同土地利用方式(耕地、退耕还林/还草)和施肥措施(化肥、有机肥)的长期定位试验土壤,分析土壤有机碳(SOC)、土壤无机碳(SIC)和全氮(TN)含量和储量,结合13C和15N稳定同位素方法,研究土地利用方式和施肥措施对于该地区土壤碳氮转化的影响规律。研究表明,退耕还灌/还草后,SOC储量较耕地均有显著提高(提高幅度0.60~0.98 Mg hm-2 a-1),SIC储量也增加或保持相同水平(柠条地除外)。相比不施肥处理,施用有机肥能显著增加SOC(1.08~1.19 Mg hm-2 a-1),施化肥处理则会降低SIC(0.06~0.16 Mg hm-2 a-1),且主要影响次生碳酸盐。施肥SIC中原生碳酸盐比例(0~23%)低于自然土壤(3%~29%)。施肥措施对于土壤碳氮的转化强度远大于土地利用方式的改变。对于内蒙等干旱半干旱地区土壤,土地利用和施肥措施对于土壤有机和无机碳的影响应该在区域固碳管理中给予全面考虑。     

Redistribution of Different Organic Carbon Fractions in the Soil Profile of a Typical Chinese Mollisol with Land-Use Change

Soil organic carbon (SOC) content depends significantly upon changes in land use and vegetation cover. This study aimed to examine the redistribution of whole soil OC, water-soluble OC (WSOC), and different density-separated OC fractions in soil profiles of 0–100 cm under different land uses and to elaborate the mechanism of C sequestration in response to the land use change. The land use types include maize plots with or without chemical fertilizer application (i.e., Maize-nitrogen, phosphorus, and potassium (NPK) and Maize-NF plots), plots with vegetation removed (No Vegetation), plots with grass (Grass), and alfalfa plant (Alfalfa). These plots used to be maize cropping system with NPK fertilizer for many years before 2003. Significant difference in SOC content generally occurred in soil layers of 0–40 cm among the different plots after 11 years of land-use change. Long-term continuous maize planting decreased SOC content; the significant SOC decrease occurred in Maize plot in the range of 9.3–23.4% for different soil layers compared with the initial soil sampled in 2003. In addition, SOC in Maize plot decreased by 3.6% and 8.5% at top two soil layers, respectively, in comparison with No Vegetation plot. The similar reduction of OC was observed in heavy OC fractions. The calculated sensitivity index for OC decreased in the order of light fraction > water-soluble fraction > the whole soil > heavy fraction. Therefore, the young and labile carbon fractions are much sensitive to land use change relative to the old and recalcitrant carbon fractions. This study indicated that land use changes led to a redistribution of SOC in soil profile, particularly at top soil layers, and conversion from arable land to natural grass cover or nitrogen-fixation plant cultivation such as alfalfa led to the enrichment of SOC at different depths of soil profile.     

Is thermal oxidation at different temperatures suitable to isolate soil organic carbon fractions with different turnover?

Findings of previous studies suggest that there are relations between thermal stability of soil organic matter (SOM), organo‐mineral associations, and stability of SOM against microbial decay. We aimed to test whether thermal oxidation at various temperatures (200°C, 225°C, 275°C, 300°C, 400°C, or 500°C) is capable of isolating SOM fractions with increasing stability against microbial degradation. The investigation was carried out on soils (Phaeozem and Luvisol) under different land‐use regimes (field, grassland, forest). The stability of the obtained soil organic carbon (SOC) fractions was determined using the natural‐¹³C approach for continuously maize‐cropped soils and radiocarbon dating. In the Luvisol, thermal oxidation with increasing temperatures did not yield residual SOC fractions of increasing microbial stability. Even the SOC fraction resistant to thermal oxidation at 300°C contained considerable amounts of young, maize‐derived C. In the Phaeozem, the mean ¹⁴C age increased considerably (from 3473 y BP in the mineral‐associated SOC fraction to 9116 y BP in the residual SOC fraction after thermal oxidation at 300°C). An increasing proportion of fossil C (calculated based on ¹⁴C data) in residual SOC fractions after thermal oxidation with increasing temperatures indicated that this was mainly due to the relative accumulation of thermally stable fossil C. We conclude that thermal oxidation with increasing temperature was not generally suitable to isolate mineral‐associated SOC fractions of increasing microbial stability.