表面活性剂LAS对土壤Cd形态及Cd在黄豆植株中分配的影响

A pot experiment was conducted to study the effect of an anionic surfactant linear alkylbenzene sulfonate （LAS） application on cadmium （Cd） fractions in soils and Cd distribution in different tissues of soybean （Glycine max） plants as well as soil pH. Soil samples were treated with three levels of Cd （0, 5, and 10 mg kg^-1） and five levels of LAS （0, 5, 15, 50, and 100 mg L^-1）. Results indicated that compared to the control soils （no Cd and no LAS treatment）, soil pH increased and available Cd decreased in the soil treated with external Cd and watered with LAS solutions. Meanwhile, soil exchangeable Cd and Cd bound to carbonates decreased; Cd bound to amorphous iron and manganese oxides and Cd bound to organic matter increased. In addition, LAS application could reduce enrichment of Cd in soybean plants, resulting in decreased Cd in the soybean plants. Thus, suitable LAS application could decrease bioavailability and mobility of soil Cd.     

七种钾释放动力学方程的比较和动力学参数的应用

Batch equilibrium experiments were conducted to investigate cadmium （Cd） sorption by two permanent-charge soils, a yellow-cinnamon soil and a yellow-brown soil, and two variable-charge soils, a red soil and a latosol, with addition of selected organic acids （acetate, tartrate, and citrate）. Results showed that with an increase in acetate concentrations from 0 to 3.0 mmol L^-1, Cd sorption percentage by the yellow-cinnamon soil, the yellow-brown soil, and the latosol decreased. The sorption percentage of Cd by the yellow-clnnamon soil and generally the yellow-brown soil （permanent-charge soils） decreased with an increase in tartrate concentration, but increased at low tartrate concentrations for the red soil and the latosol. Curves of percentage of Cd sorption for citrate were similar to those for tartrate. For the variable-charge soils with tartrate and citrate, there were obvious peaks in Cd sorption percentage. These peaks, where organic acids had maximum influence, changed with soil type, and were at a higher organic acid concentration for the variable-charge soils than for the permanent charge soils. Addition of cadmium after tartrate adsorption resulted in higher sorption increase for the varlable-charge soils than permanent-charge soils. When tartrate and Cd solution were added together, sorption of Cd decreased with tartrate concentration for the yellow-brown soil, but increased at low tartrate concentrations and then decreased with tartrate concentration for the red soil and the latosol.     

重金属和有机污染物在修饰土中的吸附

Sorption characteristics of both an organic pollutant （phenol） and a heavy metal （cadmium ion） on the clay layer of a Lou soil （Eum-orthic Anthrosol in Chinese Soil Taxonomy） along with the sorption mechanism were investigated using three soil treatments： modification with a cationic surfactant cetyltrimethylammonium bromide added at an amount equivalent to 50% and 100% of the soil CEC （50? and 100?）, modification with an amphoteric surface-modifying agent dodecyldimethylbetaine （commercially known as BS-12） added at an amount equivalent to 50% and 100% of the soil CEC （50% BS and 100%BS）, and an unmodified control （CK）. Results showed that the BS soil treatments increased sorption of both the heavy metal Cd^2＋ and the organic pollutant phenol. The equilibrium sorption amount of Cd^2＋ decreased in the order： 50%BS 〉 100%BS 〉 CK 〉 50? 〉 100?, with the BS soil treatments being about 1.3 to 1.8 times higher and the CB soil treatments about 23% to 41% lower than CK. Both the single-site and two-site Langmuir models could be applied to describe the sorption of Cd^2＋ in each soil treatment. The equilibrium sorption amount of phenol on the soil samples decreased in the order： 100? 〉 50? 〉 100%BS 〉 50%BS 〉 CK, with the CB soil treatments being 41.0 to 79.6 times higher and the BS soil treatments 4.0 to 8.3 times higher than CK. The Freundlich equation could also be used to describe the sorption characteristics of phenol. In the BS soil treatments, both an organophobic long carbon chain and hydrophilic charged groups resulted in a relatively strong sorption ability for both heavy metals and organic pollutants. In addition, the sorption ratio K, the ratio of phenol sorption amount of the modified soil to that of CK, increased initially and decreased later with the amount of phenol added, and the critical sorption ratio Kc, the peak value of the sorption ratio curve plotted against the added phenol concentration, was a good index for evaluating the sorption ability of phenol in the soil.     

A review of cadmium sorption mechanisms on soil mineral surfaces revealed from synchrotron-based X-ray absorption fine structure spectroscopy: Implications for soil remediation

The sorption of cadmium(Cd) is one of the most important chemical processes in soil, affecting its fate and mobility in both soil and water and ultimately controlling its bioavailability. In order to fundamentally understand the sorption/desorption of Cd in soil systems, X-ray absorption fine structure spectroscopy(XAFS) has been applied in numerous studies to provide molecular-level information that can be used to characterize the surface adsorption and precipitation reactions that Cd can undergo. This information greatly improves our current knowledge of the possible chemical reactions of Cd in soil. This paper critically reviews the mechanisms of Cd sorption/desorption at the mineral-water interface based on XAFS studies performed over the past twenty years. An introduction to the basic concepts of sorption processes is provided, followed by a detailed interpretation of XAFS theory and experimental data collection and processing,ending finally with a discussion of the atomic/molecular-scale Cd sorption mechanisms that occur at the soil mineral-water interface. Particular emphasis is placed on literature that discusses Cd adsorption and speciation when associated with iron, manganese, and aluminum oxides and aluminosilicate minerals.Multiple sorption mechanisms by which Cd is sorbed by these minerals have been found, spanning from outer-sphere to inner-sphere to surface precipitation,depending on mineral type, surface loading, and pH. In addition, the application of complementary techniques(e.g.,113 Cd nuclear magnetic resonance(NMR) and molecular dynamics simulation) for probing Cd sorption mechanisms is discussed. This review can help to develop appropriate strategies for the environmental remediation of Cd-contaminated soils.     

Effect of controlled-release urea on heavy metal mobility in a multimetal-contaminated soil

Controlled-release N fertilizers can affect the availability of heavy metals in the contaminated paddy soil.A soil incubation experiment was conducted to investigate the effects of prilled urea(PU),S-coated urea(SCU),and polymer-coated urea(PCU)on the solubility and availability of heavy metals Cd,Pb,Cu,and Zn in a multimetal-contaminated soil.The results showed that the application of different coated urea significantly affected the solubility and availability of heavy metals.At 5 d of incubation,the application of PU,SCU,and PCU had significantly decreased the concentrations of water-soluble and available Cd,Pb,Cu,and Zn,when compared with the control.At 60 d of incubation,the depletory effects of PU on water-soluble and available heavy metals had reduced,and the initial decrease in the concentrations of water-soluble Cd,Pb,Cu,and Zn caused by SCU had changed to an increase.The concentrations of water-soluble Pb,Cu,and Zn in the SCU-treated soil were higher than those in the control.Application with PCU led to a higher water-soluble Cu than that in the control,while the available Cd,Pb,and Zn were lower than those in the control.The effect of different coated urea was much stronger on the water solubility of the heavy metals than on their availability.The effects of controlled-release urea on the transformation of heavy metals resulted in changes in the concentrations of NH4^+,water-soluble SO4^2-,and soil p H.The results further suggested that PCU could be used in dry farming operations in multimetal-contaminated acid soils.     

可溶性有机物对土壤中绿麦隆吸附与解吸的影响

A batch equilibrium techniques was used to examine the effect of dissolved organic matter （DOM） extracted from both non-treated sludge （NTS） and heat-expanded sludge （HES） on the sorption and desorption of chlorotoluron （3-（3-chloro-p-tolyl）-1,1-dimethylurea） in two types of soils, a yellow fluvo-aquic and a red soil from China. Without DOM,sorption of chlorotoluron was significantly greater （P 〈 0.05） in the red soil than in the yellow fluvo-aquic soil. However,with DOM the effect was dependent on the soil type and nature of DOM. Chlorotoluron sorption was lower in the yellow fluvo-aquic soil than in the red soil, suggesting that with the same DOM levels the yellow fluvo-aquic soil had a lower sorption capacity for this herbicide. Application of DOM from both NTS and HES led to a general decrease in sorption to the soils and an increase in desorption from the soils. Desorption of chlorotoluron also significantly increased （P 〈 0.05） with an increase in the DOM concentration. Additionally, for sorption and desorption, at each DOM treatment level the NTS treatments were significantly lower （P 〈 0.05） than the HES treatments. This implied that non-treated sludge had a greater effect on the sorption and desorption of chlorotoluron than heat-expanded sludge.     

施入粉煤灰和污泥对酸性淋溶土镉和铅吸附的影响

The safe recycling of fly ash (FA) and sewage sludge (SS) in the agricultural processes comprises an important environmental technology on waste management. Soils amended with FA and SS may change their ability to adsorb heavy metals due to either increase of soil pH or decomposition of sludge-borne organic matter. Thus, Cd and Pb sorption was investigated by 1-month incubation under soil moisture content at field capacity and room temperature with an acidic Typic Haploxeroalf from central Greece amended with varying amounts of FA and SS. Batch experiments were conducted by equilibrating the soil samples with CaCl₂ solutions containing 0-400 mg Pb L^-1 or 0-100 mg Cd L^-1. The results showed that the Freundlich equation described well the Cd and Pb sorption. Distribution coefficient (K_d) values of Pb were higher than those of Cd in all the treatments of this study. Application of FA increased K_d values for Cd and Pb to 8.2 and 2.3 times more than the controls, respectively. Simultaneous applications of FA and SS caused a K_d increase of 3.8 and 2.1 times compared to the treatments that received only SS for Cd and Pb, respectively. Treatment of SS alone did not significantly change Cd and Pb sorption compared to the controls. The sorption reactions seemed to be mainly affected by soil pH, which was revealed by the significant correlations of Cd and Pb sorption with soil pH. These suggested that fly ash was very useful as a low-cost adsorbent for Cd and Pb and could be used as an ameliorant for biosolid-amended acidic soils.     

外源铬对小白菜(Brassica chinensis L.)根际土壤微生物生物量和磷脂脂肪酸的剖面分布特征的影响

The effects of root activity on microbial response to cadmium （Cd） loading in the rhizosphere are not well understood. A pot experiment in greenhouse was conducted to investigate the effects of low Cd loading and root activity on microbial biomass and community structure in the rhizosphere of pakchoi （Brassica chinensis L.） on silty clay loam and silt loamy soil. Cd was added into soil as Cd（NO3）2 to reach concentrations ranging from 0.00 to 7.00 mg kg-1. The microbial biomass carbon （MBC） and community structure were affected by Cd concentration, root activity, and soil type. Lower Cd loading rates （〈 1.00 mg kg-1） stimulated the growth of pakchoi and microorganisms, but higher Cd concentrations inhibited the growth of microorganisms. The content of phospholipid fatty acids （PLFAs） was sensitive to increased Cd levels. MBC was linearly correlated with the total PLFAs. The content of general PLFAs in the fungi was positively correlated with the available Cd in the soil, whereas those in the bacteria and actinomycetes were negatively correlated with the available Cd in the soil. These results indicated that fungi were more resistant to Cd stress than bacteria or actinomycetes, and the latter was the most sensitive to Cd stress. Microbial biomass was more abundant in the rhizosphere than in the bulk soil. Root activity enhanced the growth of microorganisms and stabilized the microbial community structure in the rhizosphere. PLFA analysis was proven to be sensitive in detecting changes in the soil microbial community in response to Cd stress and root activity.     

草酸对土壤和矿物中钾素的释放规律研究

Oxalic acid plays an important role in improving the bioavailability of soil nutrients. Batch experiments were employed to examine the influences of oxalic acid on extraction and release kinetics of potassium （K） from soils and minerals along with the adsorption and desorption of soil K^＋. The soils and minerals used were three typical Chinese soils, black soil （Mollisol）, red soil （Ultisol）, and calcareous alluvial soil （Entisol）, and four K-bearing minerals, biotite, phlogopite, muscovite, and microcline. The results showed that soil K extracted using 0.2 mol L^-1 oxalic acid was similar to that using 1 mol L^-1 boiling HNO3. The relation between K release （y） and concentrations of oxalic acid （c） could be best described logarithmically as y = a ＋ blogc, while the best-fit kinetic equation of K release was y = a ＋ b√t, where a and b are the constants and t is the elapsed time. The K release for minerals was ranked as biotite 〉 phlogopite 〉〉 muscovite 〉 microcline and for soils it was in the order： black soil 〉 calcareous alluvial soil 〉 red soil. An oxalic acid solution with low pH was able to release more K from weathered minerals and alkaline soils. Oxalic acid decreased the soil K^＋ adsorption and increased the soil K^＋ desorption, the effect of which tended to be greater at lower solution pH, especially in the red soil.     

pH Effect on kinetics of Heavy Metal Sorption in Soils

The pH effect on the sorption kinetics of heavy metals in soils was studied using a constant flow leaching method.The soil samples were red soil collected from Yingtan,Jiangxi,and yellow-brown soil from Nanjing,Jiansu,The heavy metals tested were zinc and cadmium.Assuming that the experimental data diffed to the following kinetic rate equation:1/c.dx/dt=kx∞-kx,the rate constant k of sorption could be determined from the slope of the straight line by plotting of 1/c,dx/dt vs.x.The results showed that the pH effect on the rate constants of heavy mental sorption in soils was very significant.The values of k decreased with increasing pH.The sorptions were more sensitive to pH in red soil than in yellow-brown soil.     

不同生育期冬小麦光谱特征对叶绿素和氮素的响应研究

研究测定了不同施氮水平条件下冬小麦冠层在七个典型生育期叶片叶绿素、地上部分全氮含量以及冠层光谱,分析了单波段反射率、可见光和近红外波段组合而成的归一化植被指数(NDVI)、比值植被指数(RVI)与相应时期叶片叶绿素和地上部分全氮含量的相关性。结果表明,施氮量增加,两个农学参量、冠层近红外波段反射率都随之增加,但当施氮量增加到300kg hm-(2一次性施入)时,上述各项指标均降低;整个生长期中孕穗期在近红外区域反射率最高,与可见光波段反射率相差最大;除分蘖期外,其它时期单波段510nm～1100nm反射率、NDVI、RVI与叶绿素和全氮含量显著相关,植被指数的相关性较单波段高,且从分蘖期到乳熟期,相关性逐渐增强;整体来讲,可见光中560nm、660nm和近红外760nm、1100nm和1200nm组合的NDVI在各生育期与两个农学指标的相关性较好,选择NDVI(560,760)可以准确拟合叶片叶绿素和地上部分全氮含量。     

不同土壤水分条件下玉米叶片/冠层气孔导度的光谱监测模型

通过玉米水分控制试验,测定不同水分条件下各生育期叶片气孔导度、叶面积指数和冠层光谱反射率等,以分析玉米叶片气孔导度的变化规律及其与光谱植被指数的相关性,从而建立基于光谱植被指数和土壤湿度的叶片气孔导度模型。结果表明：玉米在可见光区和近红外中、长波区的反射率随着土壤水分的降低而上升,但叶片气孔导度（Gs）、叶面积指数（LAI）、比值植被指数（RVI）和归一化植被指数（NDVI）随着土壤水分的下降而降低;玉米NDVI和RVI与单叶片和冠层气孔导度均呈极显著指数函数关系（P〈0.01）,且对单叶片气孔导度的拟合效果优于对冠层导度的拟合效果,而经土壤湿度订正的RVI监测模型优于NDVI监测模型。表明通过测定冠层反射光谱率可实时、迅速地定量监测玉米叶片的气孔导度,为大面积作物气孔导度估算奠定基础。     

镁(Ⅱ): 水溶态镁在中国粘土上的吸附及复合物形成的稳定常数

Techniques for measurement of the N status of rice can be an aid to making manaement decisions with economic and environmental implications.A field experiment was conuced to identify spectral variables most sensitive to N deficiency detection in rice canopy with the possibiliy for their use as a management tool. Spectral and agronomic measurements were collected in the evaluation experiment of N status from rice canopy under vive N treatments in a silt loam soil ,Nitroen fertilization effects were seen across the entire wavelength measured .Red refectance decreased and near infrared reflectance increased with increasing N fertilizer application.Spectral differences between treatments were seen throughout the test period.The naer infrared refectnce/red reflectance ration (RVI) differed mored between treatment than between single bands.Variations in canopy reflectances due to other environmental factors were reduced by the use of RVI.In the spectral variables examined ,the RVI separated the treatments most effectively,and three or four treatments can be separated.Differences in spetral responses betwenn the treatments were attributable to leaf area index ,leaf chlorophyll concentration and phtomass,wich all changed with N fertilization.     

水稻微分光谱和植被指数的作用探讨

通过两年两个不同品种水稻在不同供氮水平下的田间试验，测定了水稻冠层在生长过程中不同时期的高光谱反射率及对应的叶绿素、类胡萝卜素含量，分析了微分光谱对消除水稻冠层光谱的背景影响和植被指数在农学参数测定中的作用。结果表明：由微分光谱所得的红边位置、红边斜率与盖度和供氮水平之间有一定的相关性，与叶面积指数、地上生物量及冠层叶绿素、类胡萝卜素含量有显著相关；水稻多光谱植被指数RVI、NDVI与叶面积指数LAI及其地上部生物量之间有极显著相关性；高光谱植被指数及其变量与植被盖度、供氮水平、叶面积指数、地上生物量及叶绿素、类胡萝卜素含量之间存在相关性。这表明，用微分光谱技术与植被指数方法来监测水稻的色素含量和长势应该是可行的。     

不同品种夏玉米光谱特征差异及其与农学参量相关性研究

研究测定了不同品种夏玉米在六个典型生育期地上部分全氮、叶绿素含量、干生物量、叶面积指数(LAI)以及冠层光谱反射率,利用不同方法比较了不同品种玉米光谱特征的差异,系统分析了单波段反射率、可见光和近红外波段组合而成的8种常见植被指数与相应时期不同农学参量的相关性。结果表明:不同品种夏玉整个生育期不同品种冠层在可见光范围内反射率差异一直没有达到显著水平,苗期和拔节期在近红外区域反射率也无显著差异,从孕穗期到乳熟期在近红外区域反射率差异增大,普遍呈显著性差异。采用F检验法可以准确地反映不同品种在不同波段反射率的差异。常见光谱植被指数从拔节期到孕穗期绝大部分都可以准确反映不同品种夏玉米的农学参量,以GRVI最佳,它不仅可以区分不同品种夏玉米在中后期的长势,又可以准确拟合这段时期作物的农学参量。     

Nondestructive estimation of bok choy nitrogen status with an active canopy sensor in comparison to a chlorophyll meter

Precise estimation of vegetable nitrogen (N) status is critical in optimizing N fertilization management. However, nondestructive and accurate N diagnostic methods for vegetables are relatively scarce. In our two-year field experiment, we evaluated whether an active canopy sensor (GreenSeeker) could be used to nondestructively predict N status of bok choy (Brassica rapa subsp. chinensis) compared with a chlorophyll meter. Results showed that the normalized difference vegetation index (NDVI) and ratio vegetation index (RVI) generated by the active canopy sensor were well correlated with the aboveground biomass (AGB) (r=0.698-0.967), plant N uptake (PNU) (r=0.642-0.951), and root to shoot ratio (RTS) (r=-0.426 to -0.845). Compared with the chlorophyll meter, the active canopy sensor displayed much higher accuracy (5.0%-177.4% higher) in predicting AGB and PNU and equal or slightly worse (0.54-1.82 times that of the chlorophyll meter) for RTS. The sensor-based NDVI model performed equally well in estimating AGB (R²=0.63) and PNU (R²=0.61), but the meter-based model predicted RTS better (R²=0.50). Inclusion of the days after transplanting (DAT) significantly improved the accuracy of sensor-based AGB (19.0%-56.7% higher) and PNU (24.6%-84.6% higher) estimation models. These findings suggest that the active canopy sensor has a great potential for nondestructively estimating N status of bok choy accurately and thus for better N recommendations, especially with inclusion of DAT, and could be applied to more vegetables with some verification.     

玉米冠层叶片光谱反射率与玉米长势空间变异的关系

为了寻找一种快捷获取玉米叶绿素空间分布信息的方法,研究了玉米苗期冠层叶片光谱反射率的变化特征,分析了不同生长阶段冠层叶片光谱反射率的空间分布和叶绿素质量浓度的空间分布.研究结果表明:玉米苗期冠层叶片叶绿素的质量浓度空间分布不均匀.冠层叶片的叶绿素质量浓度与光谱指数RVI的相关性较低,与归一化差异植被指数NDV1值和550 nm波段的反射率均有较高的相关性.分析550nm波段的反射率值与冠层叶片叶绿素质量浓度值的相关关系,显示二者成负相关变化趋势,相关系数绝对值在0.69～0.88范围随着生长期的变化而逐渐增大,五叶期前期的相关性系数绝对值达到苗期最大值,表明550 nm波段反射率值能够较好的反映玉米苗期叶绿素质量浓度的水平,分析和掌握550 nm波段反射率值的变化及其空间分布特性对快速监测玉米苗期生长状况,对指导田间施肥具有重要意义.     

Spectral indices measured with proximal sensing using canopy reflectance sensor, chlorophyll meter and leaf color chart for in-season grain yield prediction of basmati rice

A number of optical sensing tools are now available and can potentially be used for refining need-based fertilizer nitrogen (N) topdressing decisions. Algorithms for estimating field-specific fertilizer N needs are based on predictions of yield made while the crops are still growing in the field. The present study was conducted to establish and validate yield prediction models using spectral indices measured with proximal sensing using GreenSeeker canopy reflectance sensor, soil and plant analyzer development (SPAD) chlorophyll meter, and two different types of leaf color charts (LCCs) for five basmati rice genotypes across different growth stages. Regression analysis was performed using normalized difference vegetation index (NDVI) recorded with GreenSeeker sensor and leaf greenness indices measured with SPAD meter and LCCs developed by Punjab Agricultural University, Ludhiana (India) (PAU-LCC) and the International Rice Research Institute, Philippines (IRRI-LCC). The exponential relationship between NDVI and grain yield exhibited the highest coefficient of determination (R²) and minimum normalized root mean square error (NRMSE) at panicle initiation stage and explained 38.3%-76.4% variation in yield using genotype-specific models. Spectral indices pooled for different genotypes were closely related to grain yield at all growth stages and explained 53.4%-57.2% variation in grain yield. Normalizing different spectral indices with cumulative growing degree days (CGDD) decreased the accuracy of yield prediction. Normalization with days after transplanting (DAT), however, did not reduce or improve the predictability of yield. The ability of each model to predict grain yield was validated with an independent dataset collected from two experiments conducted at different sites with a range of fertilizer N doses. The NDVI-based genotype-specific models exhibited a robust linear correlation (R²=0.77, NRMSE=7.37%, n=180) between observed and predicted grain yields only at 35 DAT (i.e., panicle initiation stage), while the SPAD, PAU-LCC, and IRRI-LCC consistently provided reliable predictions (with respective R² of 0.63, 0.60, and 0.53 and NRMSE of 10%, 10%, and 13.6%) even with genotype invariant models with 900 data points obtained at different growth stages. The study revealed that unnormalized values of spectral indices, namely NDVI, SPAD, PAU-LCC, and IRRI-LCC, can be satisfactorily used for in-season estimation of grain yield for basmati rice. As LCCs are very economical compared with chlorophyll meters and canopy reflectance sensors, they can be preferably used by small farmers in developing countries.     

应用IKONOS卫星影像监测冬小麦氮营养状况

应用从高精度卫星影像获取的光谱植被指数和单波段光谱反射值与冬小麦拔节期氮素营养状况进行了相关分析,结果发现:高精度卫星光谱归一化植被指数、绿植被指数、比值植被指数、优化土壤调节植被指数和单波段光谱反射值红外波段与拔节期小麦叶片叶绿素仪读数、茎基部硝酸盐含量、地上部生物量和地上部吸氮量等都有显著的线性正相关关系,相关系数范围在0.651~0.860之间。而红光波段、绿光波段则与拔节期小麦氮营养诊断指标呈显著线性负相关关系,相关系数范围在-0.803~-0.574之间。且上述光谱植被指数和单波段反射值均与小麦拔节期优化施氮量有很好的相关关系。因此,可以应用高精度卫星影像结合地面土壤植株测试进行冬小麦拔节期的冠层氮营养诊断和氮肥推荐。     

基于夏玉米光谱特征的叶绿素和氮素水平及氮肥吸收利用研究

本文首先分析不同施 N 水平条件下夏玉米冠层在6个典型生育期的光谱特征曲线,然后计算了可见光和近红外波段光谱反射率组成的归一化植被指数(NDvI)及相应时期叶片叶绿素含量和地上部分全N含量2个重要指标以及孕穗期冠层NDVI和几个N肥吸收利用效率的相关性.结果表明,孕穗期冠层光谱反射率在近红外区域反射率最大,且其可见光和近红外区域反射率差异最大:从苗期到孕穗期,NDVI和叶绿素、全N含量的相关性逐渐增强,到抽雄期减弱,灌浆期又有所增强;整体来讲绿色归一化植被指数 GNDNI(560,760)在各生育期与不同农学参量的相关性比其他波段组合的指数好,其次为NDVI(660,760)波段组合.随着施N量的增加,N肥利用效率、收获指数和N肥收获指数、N素农艺效率以及N肥回收效率均逐渐降低,对各N肥吸收利用指数的预测以NDVI(660,760)、NDVI(660,1100)和GNDVI(560,760)较理想.