不同施肥处理对黑土土壤呼吸的影响

基于中国科学院海伦生态实验站的长期定位试验,采用静态箱式法研究了玉米生长期间不同施肥处理对黑土土壤呼吸的影响。结果表明,在玉米生长期间,土壤呼吸速率表现出明显的季节性变化,分别在出苗后23、37、50、63、87、110 d出现峰值,其中最大峰值出现在出苗后第87天,其后土壤呼吸速率呈下降趋势,直到玉米收获,而根际呼吸速率的季节性变化规律与土壤呼吸相似,土体呼吸速率则主要受气温变化影响;玉米生长显著影响土壤呼吸,土壤呼吸速率的变化基本与玉米生长规律相一致,随生长而增加,随衰老而减小;施肥对土壤呼吸速率、根际呼吸速率有明显的影响,但对土体呼吸速率影响较小,从整个玉米生长期来看,NPKOM处理的土壤呼吸速率和根际呼吸速率最高,其中NPKOM处理土壤呼吸速率为C 27.5~474 mg m-2h-1,NPK处理和NP处理变化范围相近,分别为C 25.9~339 mg m-2h-1和C 29.5~358 mg m-2h-1,NK处理与CK处理变化范围分别为C 28.4~208 mg m-2h-1和C 22.1~184 mg m-2h-1;施肥对土壤呼吸量和根际呼吸量有显著的影响,表现为NPKOM>NPK>NP>CK>NK;在整个玉米生育期中,土壤呼吸累积量在拔节孕穗期和乳熟期出现两个峰值,表现为双峰曲线的变化规律,而土体呼吸累积量只在拔节孕穗期出现峰值,呈抛物线型,根际呼吸量在苗期最低,乳熟期最高,乳熟期后,根际呼吸量下降。     

玉米生长中的土壤呼吸及其受氮肥施用的影响

运用盆栽试验研究了玉米生长和施氮水平(N 150 mg kg-1和300 mg kg-1)对土壤呼吸的影响。结果表明种植玉米的土壤呼吸速率(C)的变化范围为19. 6 ~ 762. 1 mg m-2h-1,而裸土为4. 3 ~ 36mg m-2h-1。在玉米生长的条件下,苗期土壤呼吸最低,73%的土壤呼吸分配在拔节孕穗期和成熟期。玉米生长中各阶段根际呼吸对土壤呼吸的贡献在58%~98%,苗期最小。施氮对裸土呼吸速率无显著影响;在玉米生长的条件下,施用高氮的土壤呼吸比施用低氮高28%,且两种施氮水平下土壤呼吸的差异主要发生在生长中后期。玉米生长的条件下土壤呼吸与温度的相关性不显著,而裸土下土壤呼吸速率与气温、表土温度、5 cm土壤温度均呈极显著的相关性;裸土施用高氮下的土壤呼吸与温度的相关性大于低氮。总之,玉米生长和土壤施氮不仅影响土壤呼吸速率和呼吸量,也影响土壤呼吸在各生长阶段的分配,还影响到土壤呼吸与温度的关系。     

干旱区灌溉及施肥措施下棉田土壤的呼吸特征

为探讨干旱区不同灌溉方式及施肥措施对棉田土壤呼吸速率的影响及其与水热的关系,在新疆绿洲农田棉花生长季设滴灌和漫灌2种灌溉方式,每种灌溉方式设有机肥(OM)、氮磷钾化肥(NPK)、氮磷钾化肥与有机肥配施(NPK+OM)3种施肥处理,以不施肥处理为对照(CK),对不同灌溉方式及施肥措施下土壤呼吸速率及土壤温度、土壤含水率进行了测定和分析。结果表明,不同灌溉方式及施肥措施下,棉田土壤呼吸速率具有明显的季节变化特征,在7月中旬达到峰值,10月中旬降至最低;日变化呈单峰曲线,盛花期峰值出现在15:00～17:00,盛铃期峰值均出现在15:00,最低值均出现在04:00。滴灌各施肥处理平均土壤呼吸速率显著大于漫灌,施肥处理间平均土壤呼吸速率大小顺序为(NPK+OM)>OM>CK>NPK,灌溉与施肥互作条件下,滴灌方式下NPK+OM处理平均土壤呼吸速率最大。滴灌和漫灌方式下各施肥处理的温度敏感性系数(Q10值)平均值分别为2.03和2.43,不同年份漫灌Q10值均大于滴灌。不同灌溉方式及施肥措施下,用复合方程预测土壤呼吸速率的准确性较高,R2值均在0.64～0.72。综合考虑土壤温度和土壤含水率对土壤呼吸速率的影响,能够提高区域土壤呼吸作用研究的准确性。     

不同施肥条件下小麦田土壤呼吸特征研究

土壤呼吸是碳循环中的重要过程,为了解不同施肥措施对土壤呼吸过程的影响,在潮土小麦生长季研究了土壤呼吸特征.结果表明,土壤呼吸具有明显的昼夜变化和季节变化特征;土壤呼吸和土壤温度呈指数变化关系;有机肥和秸秆还田在生长后期显著增加了土壤呼吸速率,而仅仅NPK配施,减小了土壤呼吸速率.     

长期施肥下红壤旱地土壤CO2排放及碳平衡特征

在国家肥力网红壤旱地长期定位试验地上,采用静态箱/气相色谱法测定土壤CO2排放速率,同时利用根去除法区分根系对土壤呼吸的贡献,通过计算净生态系统生产力（NEP）,判断长期不同施肥下红壤旱地农田碳汇强度。结果表明,小麦、玉米生长季各处理的土壤和土体呼吸速率随着作物生长、温度升高均呈现明显的季节变化规律;玉米生长季土壤和土体累积呼吸量大于小麦生长季,小麦、玉米生长季均以NPKM处理土壤和土体呼吸累积呼吸量最大,且显著高于其它处理（P0.05）,NP和NPK处理次之,CK和NK处理最小（P0.05）;小麦、玉米生长季各处理根际呼吸占土壤呼吸的比例分别为7.6 %～17.4 %、4.7%～16.6 %,均以NPKM处理根际呼吸贡献率最大;小麦季NPKM处理、玉米季CK和NPKM处理的NEP值为负,是大气CO2的汇,且NPKM处理的净初级生产力与土壤呼吸的比值（NPP/Rs）最大,其它处理NEP值均为正,是大气CO2的源。有机无机肥配施（NPKM）相比其它处理具有较强的碳汇功能,是红壤旱地比较合理的施肥措施。     

模拟酸雨对冬小麦-大豆轮作农田土壤呼吸、硝化和反硝化作用的影响

为研究模拟酸雨对冬小麦-大豆轮作农田土壤呼吸、硝化和反硝化作用的影响,在农田进行随机区组试验,布设4个区组,每块区组随机设置4个模拟酸雨处理,分别为去离子水A1（pH=6.7）、A2（pH=4.0）、A3（pH=3.0）、A4（pH=2.0）。采用LI-8100开路式土壤碳通量测量系统对不同酸雨强度的冬小麦-大豆轮作农田进行土壤呼吸速率观测,并采用气压过程分离技术（BaPS）测定不同酸雨处理的土壤CO2产生速率、硝化速率和反硝化速率。试验结果表明,冬小麦田各处理间土壤呼吸速率无显著差异（P〉0.05）;大豆田高强度模拟酸雨A4处理明显抑制了土壤呼吸作用（P〈0.05）。就冬小麦-大豆轮作生长季而言,各处理土壤呼吸速率无显著差异（P〉0.05）,其平均土壤呼吸速率分别为（2.26±0.11）、（2.31±0.20）、（1.91±0.09）、（2.03±0.17）μmol·m-2·s-1。冬小麦田A1、A3、A4处理间土壤CO2产生速率、硝化速率和反硝化速率均无显著性差异（P〉0.05）。高强度模拟酸雨抑制了大豆田土壤CO2产生速率;大豆田A1、A3、A4处理的硝化速率测定均值分别为（191.6±36.1）、（261.6±36.3）μg·kg-1·h-1和（255.2±45.1）μg·kg-1·h-1,这3个处理的反硝化速率均值分别为（172.8±19.8）、（216.0±45.7）μg·kg-·1h-1和（216.3±44.6）μg·kg-·1h-1。研究表明,模拟酸雨强度升高未显著影响冬小麦田土壤呼吸、硝化和反硝化作用;高强度模拟酸雨（pH=2.0）降低了大豆田土壤呼吸速率和CO2产生速率,但对土壤硝化和反硝化作用有促进作用。     

长期施肥对东北中部春玉米农田土壤呼吸的影响

【目的】 探究不同施肥措施对土壤呼吸的影响,为我国东北黑土区固碳减排研究提供科学依据。 【方法】 本研究基于“国家黑土肥力与肥料效益监测基地”长期定位试验,选取不施肥 (CK)、单施化肥 (NPK)、化肥配施秸秆 (NPKS)、化肥配施低量有机肥 (NPKM1)、化肥配施高量有机肥 (NPKM2)5个不同施肥处理。采用Soil-box343土壤呼吸测量系统进行野外监测,并同时观测环境条件。 【结果】 长期不同施肥处理下,农田土壤呼吸速率变化范围为4.12～7.23 μmol/(m2·s),随玉米生长表现出“先升高后降低”的季节变化特征,最高值出现在播种后69天左右,NPKM2处理土壤呼吸速率的峰值显著高于其他处理 (P < 0.05)。监测期内土壤呼吸速率与土壤温度之间呈现显著的正相关关系,土壤温度可以解释土壤呼吸速率变异的41%～77%,土壤温度敏感系数Q ₁₀值的变化范围2.35～3.49。春玉米生长季内农田土壤呼吸总量变化范围为3473～5643 kg/hm2,NPKS处理显著高于CK处理34.2%,而NPKM2处理分别比NPKS、NPK和CK处理高21.0%、26.4%、62.4% (P < 0.05),长期有机无机肥配施处理土壤有机碳含量增加趋势比其他处理明显,截止到2016年,NPKM1和NPKM2处理SOC较初始SOC分别增加了6.01 g/kg和5.55 g/kg。 【结论】 长期施用有机肥能够增加土壤呼吸,提高土壤有机碳含量,有利于农田生产力提高和农田可持续利用。      

短花针茅荒漠草原土壤呼吸及其对施肥的响应

2011～2014年,在内蒙古短花针茅荒漠草原设置了氮、磷和氮磷配施等3种施肥处理以及单施氮肥的3个不同添加梯度处理,并以未施肥为对照,采用LI-8100土壤碳通量测量系统测定了各处理样地土壤呼吸速率季节变化,结合土壤养分、地下生物量和环境因子,分析了不同施肥处理下土壤呼吸的变化规律及其与各影响因子之间的关系。结果表明:1)土壤呼吸季节变化规律不受施肥的影响,呈单峰型,最高值出现在每年8月。2)土壤呼吸与气温和降雨量呈指数相关关系,其年际变化受降雨量的影响较大。3)单施N肥、单施P肥和NP配施均促进生长季土壤呼吸,但其作用程度受降雨量多少而不同,降雨量越高对土壤呼吸的影响越明显。4)随着N肥添加量的增加,土壤呼吸速率呈增强趋势,但土壤呼吸与环境因子间的相关性减弱;单施P处理生长季平均土壤呼吸速率最高,NP配施处理稍低,二者随气温和降雨量的变化幅度大于单施N肥处理。     

氮肥与有机肥配施对设施土壤呼吸的影响

依托设施番茄栽培连续3年田间施肥定位试验,利用田间原位土壤呼吸测定法,研究了施氮量0、187.5、375.0、562.5 kg hm~(-2)(N0、N1、N2、N3)及氮肥与有机肥(M:75000 kg hm~(-2))配施(MN0、MN1、MN2、MN3)对土壤日呼吸速率的动态变化和累积呼吸量的影响,并分析了影响土壤呼吸的因素。结果表明:在番茄生长期内,各处理土壤日呼吸速率的动态变化趋势基本一致,番茄生长前期,各施肥处理土壤日呼吸速率较小且变动较小,生长64 d后,各施肥处理土壤日呼吸速率随生长天数的延长呈快速增加趋势,82 d时达最大峰值,之后则呈现下降趋势,但仍维持较高的数值;与单施氮肥处理相比,氮肥与有机肥配施可提高土壤日呼吸速率,并极显著提高土壤呼吸累积量(P<0.01),在施氮处理中,N1处理土壤呼吸累积量显著高于其它4个处理,而氮肥与有机肥配施处理中,施氮量对土壤呼吸累积量影响不显著。各处理5 cm、10 cm、15 cm处土壤温度与土壤日呼吸速率之间均呈显著指数相关性(P<0.05或P<0.01),敏感系数Q10值均随土层深度的增加呈增大趋势,其中,N1(187.5 kg hm~(-2))和MN1处理土壤日呼吸速率对温度的敏感性较强;表层(0~10 cm)土壤容重、pH值和有机碳含量与土壤呼吸累积量之间均有显著线性关系(P<0.05)。本试验条件下,连续有机肥与氮肥配施,可显著提高土壤呼吸,促进CO2排放。     

免耕与翻耕条件下农田土壤呼吸的比较

采用开路式土壤碳通量测量系统于2010年3-10月在冬小麦-大豆轮作期对免耕与翻耕田土壤呼吸速率、5cm深土壤温度和湿度进行测定,以研究耕作措施对农田土壤呼吸的影响。结果表明,在冬小麦、大豆生长时段,免耕与翻耕田土壤呼吸速率的季节变化趋势基本一致。冬小麦生长时段免耕与翻耕田土壤呼吸速率的平均值分别为2.50±0.14和2.40±0.29μmol.m-2.s-1,大豆生长时段分别为2.82±0.28和3.50±0.52μmol.m-2.s-1。冬小麦生长时段免耕与翻耕田土壤呼吸无显著差异,但大豆生长时段二者存在显著差异(P<0.05),差异最明显的阶段在大豆开花期(7月下旬-8月中旬)。利用温度影响函数(指数函数)和湿度影响函数(二次函数)耦合的模拟模型进行土壤呼吸与土壤温度和湿度的回归分析,得出免耕条件下土壤温度和湿度可以共同解释25.3%的土壤呼吸变异(R2=0.253,P<0.05),翻耕条件下二者可以共同解释44.0%的土壤呼吸变异(R2=0.440,P<0.01)。可见,一方面,耕作措施对土壤呼吸的影响因种植作物而异,与翻耕相比,免耕显著降低了大豆田土壤呼吸,但对冬小麦田无显著影响;另一方面,免耕下土壤温度和湿度对土壤呼吸的影响比翻耕要小。     

土壤石油污染对植物苗期生长和土壤呼吸的影响

选取陕北延安地区原油和黄绵土，设计了完全混合和表面施油两种处理方式，通过盆栽试验，研究了不同浓度石油污染处理对植物生长和土壤呼吸的影响。结果表明，玉米和紫花苜蓿在完全混合的油污土壤上萌发良好，但生长受阻，而表面喷洒处理的石油污染严重影响紫花苜蓿的萌发和生长；土壤呼吸作用对石油污染十分敏感，完全混合处理土壤呼吸与石油污染比例成正相关关系，污染越重，土壤呼吸强度越大，所有污染处理1周后土壤呼吸强度出现抑制而迅速回落，并在波动中逐渐趋于稳定，但仍然显著高于对照组，表明黄土中存在对石油耐受的土著微生物群落。     

大豆生长期间的土壤呼吸

Soil respiration induced by soybean cultivation over its entire growing season and the factors influencing soil respiration were investigated to examine the seasonal pattern of soil respiration induced by soybean cultivation, explore soybean growth and photosynthesis on soil respiration, and determine the temperature dependence on soil respiration. Soil respiration in a pot experiment with and without soybean plants was sampled using the static chamber method and measured using gas chromatograph. Air temperature was a dominant factor controlling soil respiration rate in unplanted soil. Additionally, rhizosphere respiration comprised 62% to 98% of the soil respiration rate in the soybean-planted soil varying with the soybean growth stages. Harvesting aerial parts of soybean plant caused an immediate drop in the soil respiration rate at that stage. After harvesting the aerial parts of the soybean plant, a highly significant correlation between soil respiration rate and air temperature was found at the flowering stage （P 〈 0.01）, the pod stage （P 〈 0.01）, and the seed-filling stage （P 〈 0.05）. Thus, rhizosphere respiration during the soybean-growing period not only made a great contribution to soil respiration, but also determined the seasonal variation pattern of the soll respiration rate.     

Root and Microbial Contributions to Soil Respiration during a Soybean Growing Season

Soil respiration is an important process for carbon geochemical cycling. Based on our five long‐term fertilizer experiments, soil respiration was measured using pot experiments with or without planting soybean. Soil respiration rates and soybean root biomass were determined at different observation times. Soil respiration rates due to soil microbial activity could be estimated by extrapolating a newly derived regressive equation at zero root biomass. Soil microbial respiration rates in the control were also observed directly, ranging from 16.0 to 42.7 mg carbon (C) m^?2 h^?1. Average soil microbial respiration rates from the regression analyses and direct observations were 32.9 and 27.8 mg C m^?2 h^?1, respectively. The average proportions of soil respiration rates due to the soybean growth were 63.0% using the regressive equation and 69.8% from direct observation. Therefore, the application of these two methods could provide new insight for separating plant root respiration from soil microbial respiration, which is important for estimating their individual contributions to atmospheric carbon dioxide.     

Tillage and cropping effects on selected properties of an Argiudoll in Argentina

Abstract

Soils of the Argentine humid pampa region are usually weakly structured due to its high silt content. Selecting crop sequence or tillage systems are an alternative in small farms for the protection of the soil against physical degradation and erosion given that conservation practices, grass meadows, and fertilizers are expensive and therefore rarely used. Evaluation of selected soil properties was conducted on soil sampled from a long‐term tillage experiment with continuous soybean established in 1975 on a Typic Argiudoll silty loam soil in Argentina. Tillage treatments included conventional tillage with moldboard plow (CT), chisel plow (CP), and no till (NT). A comparison with continuous corn under NT was also carried out. Sampling was performed after the emergence of both crops in 1990. Tillage and cropping treatments affected properties related to soil slacking and dispersion to a greater extent than they did on aggregate size distribution. According to the De Leenheer and De Boodt index, aggregate stability within soybean soil classified as bad for CT, unsatisfactory for CP, and very good for NT, whereas the soil with corn under NT classified as excellent. The no tillage treatment within soybean had significantly more organic carbon in the 0–5 cm depth than CP or CT. Soil respiration was significantly higher in NT than in CT in the surface layer, while CT showed higher values in the 10–15 cm depth. Tillage treatments did not significantly affect microbial biomass under soybean cropping. The effect of monoculture corn versus monoculture soybean under NT on soil respiration, biomass and organic carbon was not significant. Soil pH in the 0–5 cm depth under soybean was in the order NT > CP > CT, whereas the soil with corn under NT was more acid than the soybean soil (P=0.05). Cation exchange capacity and exchangeable bases followed a similar trend. Organic carbon (0–5 cm depth) and aggregate stability were significantly correlated when samples from all treatments were considered.     

保护性耕作下大豆农田土壤呼吸及影响因素分析

为了探讨保护性耕作对旱作农田土壤呼吸的影响,采用LI6400-09仪器(LI6400便携式光合作用系统连接6400-09呼吸室)在重庆北碚西南大学试验农场对平作(T)、垄作(R)、平作+覆盖(TS)、垄作+覆盖(RS)、平作+覆盖+秸秆速腐剂(TSD)、垄作+覆盖+秸秆速腐剂(RSD)6种处理下的西南紫色土丘陵区小麦/玉米/大豆套作体系中大豆生长季节的土壤呼吸及其水、热、生物因子进行测定和分析,探讨西南丘陵区保护性耕作下大豆农田土壤呼吸及其影响因素。结果表明,大豆整个生育期内土壤呼吸先缓慢增强,到开花期开始增长迅速,成熟期明显下降。不同处理土壤呼吸速率存在差异,表现为TTSD>TS、R>RSD>RS,土壤呼吸的土温敏感指标Q10值排序为TS>TSD>RS=R>T>RSD。秸秆覆盖处理的土壤呼吸对于土壤温度敏感性较高,垄作则降低了土壤温度敏感性。5 cm土层的土壤含水量高低排序为TSD>RSD>TS>RS>T>R。本研究中土壤呼吸与土壤水分呈抛物线函数关系,垄作处理下土壤呼吸与土壤水分正相关,达到显著水平;其他处理均表现负相关,其中TS达到极显著水平。在大豆农田生态系统中优势类群有弹尾目、螨目和双翅目,干漏斗法、陷阱法捕获的土壤动物与土壤呼吸均没有显著的相关关系,两种方法所得土壤动物数量加总与土壤呼吸进行相关分析,发现处理T相关系数达到显著水平,r=0.901,P=0.037。     

两种轮作模式下秸秆还田对土壤呼吸及其温度敏感性的影响

通过分析不同作物轮作模式下秸秆还田对土壤呼吸及其温度敏感性的影响,为深入探究关中地区农田生态系统碳循环提供理论依据。试验设置于陕西省杨凌地区,在2012年10月至2014年9月期间以冬小麦-夏玉米轮作模式和冬小麦-夏大豆轮作模式作为研究对象,分别设置秸秆还田(SM)和秸秆不还田(NS)两个处理,测定分析不同处理下土壤呼吸、土壤温度及土壤含水量的变化趋势和差异,并估算土壤呼吸的温度敏感性(Q_(10))。结果表明:土壤呼吸存在明显的季节变化,在作物生育期大部分时间内,SM处理的土壤呼吸速率均显著高于NS处理(P0.05),且SM处理的作物生育期土壤呼吸平均速率及土壤呼吸累计排放量也极显著高于NS处理(P0.01);不同作物生育期土壤呼吸平均速率依次为夏玉米夏大豆冬小麦,土壤呼吸总量表现为冬小麦夏玉米夏大豆、冬小麦-夏玉米轮作冬小麦-夏大豆轮作。冬小麦-夏玉米轮作与冬小麦-大豆轮作的土壤温度间存在差异;其中,在冬小麦生育前期,冬小麦-夏玉米轮作的土壤温度显著高于冬小麦-大豆轮作;第2季夏玉米生育期内5 cm深度的土壤温度显著低于同季的夏大豆;相比NS处理,SM处理能提高冬季土壤的温度,并降低春季和夏季的土壤温度;在高温少雨的时期内,SM处理能够提高0~30 cm土壤的平均含水量,不同的前茬作物引起两种轮作模式中冬小麦耕作层土壤含水量间明显的差异,夏玉米耕作层土壤含水量显著高于夏大豆。相关分析表明,土壤呼吸与5 cm和10 cm土壤温度均存在极显著的正相关性,且与5 cm土壤温度的相关性更好;但土壤呼吸与0~30 cm的土壤平均含水量无显著相关性。5 cm和10 cm土壤温度变化能够分别解释土壤呼吸变化的64.6%~67.3%和51.5%~59.6%。整个研究周期内,温度敏感性(Q_(10))为1.70~2.01,冬小麦-夏玉米轮作的温度敏感性显著高于冬小麦-大豆轮作,且同一轮作模式下SM处理的温度敏感性显著低于NS处理。因此,秸秆还田能够提高农田的土壤呼吸作用,降低土壤呼吸的温度敏感性,同时能够调节土壤的水热状况。     

Influence of cultivar and germination on bioactive amines in soybeans (Glycine max L. Merril)

The levels of amines in soybeans as affected by cultivar in two consecutive years and by germination were investigated. Spermidine, spermine, putrescine, agmatine, and cadaverine were detected, whereas tyramine, histamine, tryptamine, serotonine, and phenylethylamine were not. Spermidine was the predominant amine followed by spermine. High concentrations of these amines confirmed soybean as a rich source. Cadaverine was confirmed to be inherent to soybean. The percent contribution of spermidine and spermine to total levels was not affected by cultivar in either years. However, amine levels were affected by cultivars in different ways in the consecutive years. Cadaverine was affected more by the cultivar, whereas spermidine, spermine, and agmatine were affected by harvest year. During germination the levels of amines from soybean increased significantly, except for agmatine. Spermidine and spermine accumulated in the cotyledon, whereas cadaverine and putrescine accumulated in the radicle and hypocotyl.     

Changes in some soil properties in a Vertic Argiudoll under short-term conservation tillage

The purpose of this work was to determine whether some soil physical and chemical properties, and microbial activity were affected by two conservation tillage systems in a Chernozemic clay loam soil (Vertic Argiudoll), after 5 years of trial initiation. Two crop sequences, corn (Zea mays L.)–wheat (Triticum aestivum L.)/soybean (Glycine max (L.) Merr.) and wheat/soybean, under chisel plowing (ChP) and no till (NT) were evaluated. Physical and chemical properties were also analyzed taking the same soil without disturbance as reference. The Hénin instability index (HI) was larger in ChP than in NT in both corn–wheat/soybean (C–W/S) and wheat/soybean (W/S) sequences (P≤0.05). The C–W/S sequence differed from W/S (P≤0.01) in total organic carbon (TOC). As regards organic carbon fractions, no differences were found in labile organic carbon (LOC), while W/S under ChP showed the lowest value (P≤0.01) of humified organic carbon (HOC). No differences were found in microbial respiration either in crop sequences or in tillage systems. Soil physical and chemical properties differentiated crop sequences and tillage treatments from the undisturbed soil when a Student’s t-test was performed. Five years elapsed since the beginning of this trial was time enough to detect changes in some of the soil properties as a consequence of management practices. An important reduction in the soil structural stability was observed as related to the undisturbed soil. However, the C–W/S sequence under NT resulted in lower soil degradation with respect to the other treatments.