Effect of fractionation on the enzymatic state and behaviour of enzyme activities in different structural soil units

Summary The behaviour and state of soil catalase, dehydrogenases, urease and proteases associated with different soil structural fractions were studied. Assays of the enzymatic sensitivity to pH variation, thermal stability and the calculation of kinetics constants of Michaelis were performed. The results indicated that catalase and urease activity in these soils seem to be of the same type, because the activities presented a similar behaviour in the soil fractions studied. However, their state appeared different in each group of soil units. Dehydrogenases showed a similar state and behaviour while proteases were in a different state and behaviour in each soil fraction.     

A soil quality index based on the equilibrium between soil organic matter and biochemical properties of undisturbed coniferous forest soils of the Pacific Northwest

Recent studies have suggested that the organic matter contents of undisturbed soils (under natural vegetation) are in equilibrium with biological and biochemical properties. Accordingly, we hypothesised that such equilibria should be disrupted when soils are subjected to disturbance or stress, and that measurement of this disruption can be expressed mathematically and used as a soil quality index. In this study, we evaluated these hypotheses in soils from the H.J. Andrews Experimental Forest in Oregon. Both O and A horizons were sampled from nine sites in Spring 2005 and Fall 2006. Soil samples were analyzed for enzyme activities (phosphatase, β-glucosidase, laccase, N-acetyl-glucosaminidase, protease and urease), and other biological and chemical properties including N-mineralization, respiration, microbial biomass C (MBC), soil organic carbon (SOC) and total nitrogen content. In addition, soil samples from one old-growth site were manipulated in the laboratory to either simulate chemical stresses (Cu addition or pH alteration) or physical disturbances (wet-dry or freeze-thaw cycles). The results showed variation in biological and biochemical soil properties that were closely correlated with SOC. Multiple regression analysis of SOC levels against all soil properties showed that a model containing only MBC and phosphatase activity could account for 97% of the SOC variation among the sites. The model fit was independent of spatial and temporal variations because covariates such as site, stand age, sampling date, and soil horizon were found to be not statistically significant. Although the application of stress/disturbance treatments inconsistently affected most of the individual biochemical properties, in contrast, the ratio of soil C predicted by the model (C_p), and soil C measured (C_m) was consistently reduced in soils submitted to at least one level of stress and disturbance treatments. In addition, C_p/C_m was more affected in soils submitted to wet-dry cycles and Cu contamination than to freeze-thaw cycles or shifts in soil pH. Our results confirm previous evidence of a biochemical balance in high quality undisturbed soils, and that this balance is disrupted when the soil is submitted to disturbances or placed under stress conditions. The C_p/C_m ratio provides a simple reference value against which the degrading effects of pollutants or management practices on soil quality can be assessed.     

Effect of soil organic matter, electrical conductivity and sodium adsorption ratio on tensile strength of aggregates

The properties of soils affected by salinity and processes involving degradation of soil structure have been partly recognized. However, the effects of saline and sodic conditions on mechanical and physical properties of soils have been studied to a lesser extent. In this research, the effects of electrical conductivity (EC) and sodium adsorption ratio (SAR) on soils possessing various amounts of organic matter were assessed under laboratory conditions. The soils contained a uniform clay type, predominantly Illite. The major difference of the soils was their amount of organic matter content. The treatments consisted of solutions with definite EC and SAR (two levels of EC: 0.5 and 4 dS/m and three levels of SAR: 0, 5 and 15). The amount of tensile strength was dependent on organic matter, EC, and SAR in a way that with the increase of SAR, the tensile strength decreased. In similar SAR, treatments with higher EC exhibited greater tensile strength. Also, the soils with higher organic matter showed greater tensile strength. The analysis of variance showed the significant difference (at 1%) between the mean of parameters analyzed (soil type, sampling depth, EC, and SAR). The order of averages of tensile strength were: permanent pasture (Agropyron elengatum)Festuca arusdinaceae)相似文献   

Spatial distribution and physiology of biological soil crusts from semi-arid central Spain are related to soil chemistry and shrub cover

Despite the critical role of biological soil crusts (BSCs) in arid and semi-arid ecosystem function, few studies are found concerning the most important environmental variables affecting their distribution and physiology. This study seeks to determine soil and microenvironmental factors affecting the spatial distribution and pigment production of BSC-forming lichens and mosses in open patches of a semi-arid Mediterranean kermes oak thicket. We measured late-successional BSC cover, shrub cover, distance to nearest kermes oak (to test for effects of kermes oak thicket microenvironment on BSC), and pigment concentration of one lichen (Cladonia foliacea) and one moss (Pleurochaete squarrosa) species in the Nature Reserve El Regajal-Mar de Ontígola (Central Spain). At the macroscale (>0.5 m), results showed that BSC distribution and pigments were tightly coupled to a suite of soil properties, in particular soil pH, Fe, and Ca. Specifically, soil pH had a positive relationship with the cover of five individual BSC-forming lichen species and was negatively related to pigment production in C. foliacea. When pH was excluded from the analysis, Ca appeared as the main soil variable and was correlated with total BSC cover and total lichen cover. The micronutrient Fe had a significant positive relationship with the concentration of eight pigments in P. squarrosa and was also coupled with the cover of two BSC-forming lichens. Manganese, previously proposed as a key limiting micronutrient for BSCs, affected lichen diversity in a negative way. At the microscale (∼0.5 m), kermes oak microenvironment, shrub cover, and moss cover were determinants of BSC distribution, and total lichen and total BSC cover were overrepresented on N and E-facing shrub microsites. Our findings suggest that soil chemical variability and microsite diversity created by neighbouring vegetation affect BSC distribution in complex and essential ways and that studies aiming to explore BSC-environment relationships should be conducted at various spatial scales. Studies based on species- or group-specific responses are, thus, inadequate to unveil the main factors determining the distribution of the diverse organisms that constitute BSCs and/or to propose potential tools aiming to restore BSC in arid and semiarid ecosystems.     

Spatial and temporal variability of soil hydraulic conductivity in relation to soil water distribution, using an exponential model.

An internal drainage experiment was conducted to examine soil water content variability in space and in time. It is shown that the flux-gradient model used to describe water flow in field soil profiles, based on the Darcy–Buckingham equation, yielded results of extreme variability and questionable validity. The problem lies in the representativeness of a site due to soil variability, horizontally and vertically in time, which added to the character of the hydraulic conductivity versus soil water content relation, which in many cases can be approximated by exponential functions, leads to coefficients of variation up to 170% in the estimation of soil hydraulic conductivity values.     

Impact of faunal complexity on microbial biomass and N turnover in field mesocosms from a spruce forest soil

In a field study using soil mesocosms in an acid spruce forest soil we investigated the effects of mesofauna and macrofauna on microbial biomass, dissolved organic matter, and N cycling. Intact soil monoliths were taken from the ground, defaunated by deep-freezing, and wrapped in nets of various mesh-sizes to control re-immigration of different faunal size-classes. The monoliths were then replanted in the field. Three treatments of mesocosms were prepared: (1) with only microbiota, (2) microbiota and mesofauna, and (3) microbiota, mesofauna, and macrofauna (= complex fauna). After 8 months of exposure the mesocosms and the unmanipulated control plots (treatment 4) were destructively sampled. We estimated microbial biomass by substrate-induced respiration and the chloroform fumigation-extraction method. N cycling was measured by monitoring microbial N mineralization, the NH _inf4 ^sup+ content, and selected amino acids and the activities of protease, urease, and deaminase. The results from the L/F layer showed that the pool of the microbial biomass was not changed by the activity of the mesofauna. However, the mesofauna and macrofauna together enhanced SIR. An increase in microbial N mineralization was only observed in treatment 3 (microbiota + complex fauna). Protease activity and NH _inf4 ^sup+ content increased in treatments 2 (microbiota + mesofauna) and 3 (microbiota + complex fauna). The complex fauna induced a soil pH increase in treatment 3 as opposed to treatment 1 and the control. This increase was presumably due to excretory NH _inf4 ^sup+ . Principal component analysis revealed that the complex fauna in treatment 3 caused a significantly higher N turnover per unit of microbial biomass.     

The effect of initial soil water content and rainfall intensity on near-surface soil hydrologic conductivity: A laboratory investigation

This paper investigates the influence of rainfall intensity and initial soil water content on changes in the near-surface soil hydraulic conductivity. While numerous papers have examined surface sealing, this paper outlines two important innovations: the design and application of time-domain reflectometry probes with the ability to measure and record soil water content at very short time and length scales; the design and application of stainless steel tensiometers to measure soil water potential at the same, very short, time and length scales. These permit the Richards Equation to be applied and the near-surface hydraulic conductivity computed. An exponential curve was fitted to the temporal changes in hydraulic conductivity, as both a function of water potential or soil water content, with a high degree of fit. As rainfall intensity increased, the degree of scatter about the best-fit line increased, probably due to the ability of high intensity rainfall to disrupt the near-surface soil structure. While utilising deterministic models, such as the Richards Equation, may be appropriate at the column scale there are practical difficulties due to the very small length scale variability in each of the input variables. The experimental results detailed here indicate that even with data at very short time and length scales it is still not possible to utilize the Richards Equation, or at least, it may be difficult to transfer the laboratory results to the field due to the effect of the specific laboratory experimental conditions on the results.     

Effects of soil properties and land management on the structure of grassland snail assemblages in SW Germany

Quantitative analyses of the snail assemblages of four a priori defined categories of grassland sites, characterized by soil moisture, land use and management, were conducted in SW Germany. Topsoil pH and calcium content were determined for each of 39 sites. The objectives were to study the effects of these habitat characteristics on snail density, species richness and quantitative structure of the land snail assemblages. With one exception, no significant relationships between soil pH (as a proxy for Ca supply) and the total numbers of individuals and species at the four site categories were observed. Snail density and species richness on groundwater soils, represented by rarely mown sedge stands and regularly mown meadows, were clearly higher than on intermediate moisture soils (as defined by the climatic water balance) of regularly mown meadows, indicating that soil moisture was the most important factor accounting for these differences. Furthermore, we observed specific conditions associated with the presence of trees in the category of meadow orchards on intermediate moisture soils. There, numbers of individuals and species were significantly higher than in the meadows on intermediate moisture soils. A posteriori cluster analysis of quantitative similarity of all snail assemblages showed that soil moisture accounts for the major differences between the site categories with respect to snail density, species richness and quantitative composition of the grassland snail assemblages. As these results are in contrast to the findings of many woodland studies, we suggest that the occurrence or abundance of certain species in the site categories are explained by land management, especially by frequent disturbances at the soil surface caused by mowing.     

Dynamics of soil microbial biomass C, N and P in disturbed and undisturbed stands of a tropical wet-evergreen forest

To understand the spatial and temporal dynamics of soil microbial biomass and its role in soil organic matter and nutrient flux in disturbed tropical wet-evergreen forests, we determined soil microbial biomass C, N and P at two soil depths (0–15 and 15–30 cm), along a disturbance gradient in Arunachal Pradesh, northeastern India. Disturbance resulted in considerable increase in air temperature and light intensity in the forest and decline in the soil nutrients concentration, which affected the growth of microbial populations and soil microbial biomass. There were significant correlations between bacterial and fungal populations and microbial biomass C, N and P. Soil microbial population was higher in the undisturbed (UD) forest stand than the disturbed forest stands during post-monsoon and less during rainy season due to heavy rainfall. Greater demand for nutrients by plants during rainy season limited the availability of nutrients to soil microbes and therefore, low microbial biomass C, N and P. Microbial biomass was negatively correlated with soil temperature and pH in all the forest stands. However, there were significant positive relationships among microbial biomass C, N and P. Percentage contribution of microbial C to soil organic C was higher in UD forest, whereas percentage contribution of microbial biomass N and P to total N and total P was higher in the moderately disturbed site than in the highly disturbed (HD) site. These results reveal that the nutrient retention by soil microbial biomass was greater in the selective logged stand and would help in the regeneration of the forest upon protection. On the other hand, the cultivated site (HD) that had the lowest labile fractions of soil organic matter may recover at a slower phase. Further, minimum and maximum microbial biomass C, N and P during rainy and winter seasons suggest the synchronization between nutrient demand for plant growth and nutrient retention in microbial biomass that would help in ecosystem recovery following disturbance.     

Effects of 'Agri-SC' soil conditioner on soil structure and erodibility: some further observations

Abstract. In field and laboratory experiments the conditioner‘Agri-SC’has shown improvements in the structure of loamy sand soils in east Shropshire, UK. It resulted in statistically significant decreases in soil bulk density values and increases in soil porosity and aggregate stability. Further experiments are in progress on both loamy sand and silt loam soils.     

Water retention and hydraulic conductivity of a loamy sand soil as influenced by crop rotation and fertilization

Measurements are reported of soil organic carbon content, dry bulk density, water retention characteristics, and saturated hydraulic conductivity of a sandy loam soil with two different crop rotations and two levels of fertilization. The water retention characteristics were fitted to the van Genuchten equation. Values of unsaturated hydraulic conductivity were estimated by calculation. It was found that crop rotation has much larger effects on these soil physical properties than fertilization. Water retention and hydraulic conductivity are greater when mustard, and clover with grass are included in the crop rotation, but only at water contents greater than 0,10 and 0, 13 kg kg^?1respectívely.     

Impact of perennial pasture and tillage systems on carbon input and soil quality indicators

Soil degradation associated with tillage is a major problem in Uruguayan agriculture. Either rotation of crops with pastures (ROT) or no-till (NT) cropping have been proposed as alternatives to minimize the impact of agriculture on soil quality. The combined impact on soil properties of ROT and NT has not been evaluated. In this study, we report results of the first 12 years of a long-term experiment established on a clay loam soil in western Uruguay. The objective was to determine the influence of conventional tillage (CT) and NT on systems under continuous cropping (CC, two crops per year) or ROT (3.5-year annual crops/2.5-year pastures). Soil samples taken at the beginning of the experiment in 1994 and in 2004 were analyzed for organic carbon (SOC), total organic carbon (TSOC) and total nitrogen content (STN), and for water-stable aggregation (WAS). Soil loss and erodibility indicators were studied using microrain simulator. With 12 years, the cumulative carbon (C) inputs of aboveground biomass were similar between tillage, but C input in CC was 50% higher than ROT. This difference was explained because 84% of the pastures dry matter was consumed by animals. Nevertheless we estimated a higher below ground biomass in ROT compared to CC systems (24.9 Mg ha⁻¹ vs. 10.9 Mg ha⁻¹). NT presented 7% higher SOC than CT (0–18 cm) with no differences between rotation systems. While all treatments declined in STN during 12 years, ROT had 11% and 58% higher STN and WAS than CC systems, with a large impact of the pasture under CT. Runoff and erosion were minimized under NT in both rotations systems. Thus, including pastures in the rotation, or switching from CT to NT improved soil quality properties. The expected benefit of combining NT and ROT will likely require more years for the cumulative effect to be detectable in both C input and soil properties.     

Changes of pore morphology, infiltration and earthworm community in a loamy soil under different agricultural managements

Earthworm activity produces changes at different scales of soil porosity, including the mesoporosity (between 1.000 and 30 μm eq. dia.) where both water retention and near-saturated infiltration take place. At this scale, the structural changes are poorly described in temperate agricultural systems, so we do not yet fully understand how these changes occur. The present study was conducted to determine the relationships between the morphology of the mesopores, which is mainly affected by earthworm activity, and the hydrodynamic behaviour (near-saturated infiltration) of topsoil under different agricultural managements inducing a large range of earthworm populations.Investigations were carried out at the soil surface in three fields under different management practices giving rise to three different earthworm populations: a continuous maize field where pig slurry was applied, a rye-grass/maize rotation (3/1 year, respectively) also with pig slurry, and an old pasture sown with white clover and rye-grass.Pore space was quantified using a morphological approach and 2D image analysis. Undisturbed soil samples were impregnated with polyester resin containing fluorescent pigment. The images were taken under UV light, yielding a spatial resolution of 42 μm pixel⁻¹. Pores were classified according to their size (which is a function of their area) and their shape. Hydraulic conductivity K(h) was measured using a disc infiltrometer at four water potentials: −0.05, −0.2, −0.6, and −1.5 kPa. The abundance and ecological categories groups of earthworms were also investigated.Continuous soil tillage causes a decrease in both abundance and functional diversity (cf. maize compared with old pasture) when soil tillage every 4 years causes only a decrease in abundance (cf. rotation compared with old pasture). There were no relationships between total porosity and effective porosity at h=−0.05 kPa. Image analysis was useful in distinguishing the functional difference between the three managements. Fewer roots and anecic earthworms resulted in fewer effective tubular voids under maize. There were fewer packing voids in the old pasture due to cattle trampling. Greater hydraulic conductivity in the pasture phase of rotation may arise from a greater functional diversity than in the maize and absence of cattle trampling compared with the pasture. We point to some significant differences between the three types of agricultural management.A better understanding is required of the influence of agricultural management systems on pore morphology. This study provides a new methodology in which we consider the earthworm activity as well as community in order to assess the effects of agricultural management on soil structure and water movement.     

混凝法去除镉及含镉絮体的稳定性研究

以广西龙江河的河水和底泥为研究对象,通过混凝沉淀实验验证聚合氯化铝(PAC)对人工配制的超标40倍河水中镉的去除效果,并考察不同pH模拟洪水对含镉絮体释镉的影响。结果表明:在pH为(9.00±0.05)的浑浊河水中,PAC对河水中的镉具有很好的去除效果,投加PAC后12h,PAC对河水中镉的去除率可达到98.52%～99.42%;在pH为(6.00±0.05)～(8.00±0.05)的模拟洪水过后72h内,河水中的Cd2+浓度均低于5μg/L,含镉絮体不释镉或少量释镉,稳定性较好;在pH为(5.00±0.05)的模拟洪水过后72h内,河水中的Cd2+浓度部分高于5μg/L,含镉絮体释镉量较大,稳定性较差。因此,在龙江河的生态环境影响后评估工作中应加强洪水期的监测。     

Variability of soil physical quality and erodibility in a water-eroded cropland

Physical degradation of the soil increases its susceptibility to erosion by water action. However, relatively few studies have evaluated the opposite, i.e., the impact of water erosion on soil erodibility. This study was conducted in a corn field in Ohio. Some sites within the field have experienced water-induced soil erosion following heavy rainstorms. Physical characteristics of the soil were compared between eroded (ER) and un-eroded sites (UN). Compared with ER, the soil in UN had lower penetration resistance (4.87 vs. 4.53 MPa), bulk density (1.45 vs. 1.33 Mg m^?3), and sand content (17.4 vs. 14.2%), and higher shear strength (80.1 vs. 125.3 KPa), hydraulic conductivity (3.0 vs. 3.4 cm h^?1), intrinsic permeability (31.9 vs. 36.4 × 10^?10 cm²), and contents of soil organic carbon (36.1 vs. 32.1 g kg^?1), total nitrogen (3.3 vs. 3.1 g kg^?1), clay (25.2 vs. 24.2%), silt (60.5 vs. 58.4%), and very fine sand (3.4 vs. 1.1%). Also Munsell's variables differed between ER and UN (1.24 vs. 0.54 for hue, 4.59 vs. 4.35 for value, and 1.99 vs. 1.79 for chroma, respectively). The erodibility factor (K) was lower in UN than in ER (0.00327 vs. 0.00354 Mg ha h ha^?1 MJ^?1 mm^?1, respectively). Hence, it is suggested the ER sites within the corn field agroecosystem are more susceptible to accelerated erosion as compared with UN sites.     

Seasonal changes in multi-scale spatial structure of soil pH and related parameters along a tropical dry evergreen forest slope

Seasonal changes in multi-scale spatial variation in soil chemical properties, which may be controlled simultaneously by biotic and abiotic factors, have not been studied in tropical dry forests. We evaluated the spatial variation of physico-chemical soil properties, plant litter and terrain attributes at multiple scales in a tropical dry evergreen forest using multivariate geostatistics. Soil samples were collected at different depths using nested interval sampling during 1- and 10-m intervals in both the wet and dry seasons. We measured pH, exchangeable cations (Ex-K⁺ and Ex-Ca²⁺), acidity (Ex-H⁺ and Ex-Al³⁺), particle size (clay and sand contents), and forest floor mass (O_i and O_a). Pronounced spatial variation in pH was observed in surface soil (0-5 cm) but not in deeper soil (5-55 cm). Multi-scale spatial structures with short (20 m) and long (86 m) ranges were observed in the auto- and cross-variograms of soil, litter and slope gradient. Pronounced multi-scale structures were observed simultaneously in pH and Ex-Ca²⁺ both in the wet and dry seasons. Only a short-range structure was observed in Ex-K⁺ and O_a, whereas a long-range structure was pronounced in sand contents and slope gradients. Although the variograms had similar shapes between wet and dry seasons for almost all variables, the short-range structure of the cross-variogram between O_a with pH and base cations was more pronouncedly developed in the wet season than in the dry season. Scale-dependent correlation coefficients suggest that a small-scale spatial variation in pH was connected to heterogeneous litter accumulation via base-cation input, whereas long-range spatial variation was simultaneously linked to particle size and slope gradient. This multivariate geostatistical approach applied within a stand detected biotic and abiotic factors controlling spatial variation in soil properties at both short and long distances.     

New indices for quantifying the resistance and resilience of soil biota to exogenous disturbances

The stability (resistance and resilience to disturbance) of a soil system is a key factor influencing ecosystem properties and processes. To compare the stability of different systems, it is necessary to have indices that provide a relative quantitative measure of both the resistance and resilience of a response variable in all possible scenarios. However, the indices currently in use are frequently unable to do this, or are difficult to interpret. Here, we present new indices that avoid these problems. We compare our indices with previously published indices of stability, and also test their performance by using a real data set. We show that our indices accurately represent the response of soil properties (e.g. soil microbial biomass) to a disturbance, and that they are capable of determining differences in stability between contrasting soils.     

Estimation and analysis of soil hydraulic properties through infiltration experiments: comparison of BEST and DL fitting methods

The BEST method (Beerkan estimation of soil transfer parameters through infiltration experiments) appears promising and easy to estimate not only saturated hydraulic conductivity but also water retention and hydraulic characteristics. However, few tests have been conducted to test the methodology. This study involved field BEST infiltration experiments for three layers (surface, 15 and 30 cm) for each of three soils with different soil textures under grassland. By comparing BEST with DL (differentiated linearization method), we found that the DL method did not produce a good estimate of the soil hydraulic properties and neither did it identify the transient flow state. The BEST method resulted in reasonable results and is therefore promising. However, with BEST we encountered some anomalies when calculating hydraulic properties in some cases with too few data points under the transient flow state. We show that the application of BEST field experiments requires a wide range of soil water content from initial to saturated states so as to include sufficient transient flow. The soil hydraulic properties determined using the BEST method showed contrasting characteristics between different soil textures with higher saturated hydraulic conductivity under coarse texture and lower values under loam textures, especially with highly compacted soils. Vertical variation in soil hydraulic properties was significant, and the surface layer had a lower saturated hydraulic conductivity partly caused by compaction (high bulk density) or by remnants of grass plants. Further research on the effects of compaction and grass plants on soil hydraulic properties is needed.