Recycling of Two-Phase Olive-Mill Cake “Alperujo” by Co-composting with Animal Manures

The use of poultry manure or goat/sheep manure in the co-composting of the two-phase olive-mill cake “alperujo” (ALP) with olive leaf (OL) is compared by studying organic-matter mineralization and humification processes during composting and the characteristics of the end products. For this, two different piles (P1 and P2) were prepared using ALP with OL mixed with poultry manure (PM) and goat/sheep manure (GSM), respectively, and composted by the turned windrow composting system. Throughout the composting process, a number of parameters were monitored, such as temperature, pH, electrical conductivity (EC), organic matter (OM), OM losses, total organic carbon (C_org), total nitrogen (N_t), C_org/N_t ratio, and the germination index (GI). In both piles, the temperature exceeded 55 °C for more than 2 weeks, which ensured maximum pathogen reduction. Organic-matter losses followed a first-order kinetic equation in both piles. The final composts presented a stabilized OM and absence of phytotoxins, as observed in the evolution and final values of the C_org/N_t ratio (C_org/N_t < 20) and the germination index (GI > 50 percent). Therefore, composting can be considered as an efficient treatment to recycle this type of waste, obtaining composts with suitable properties that can be safely used in agriculture.     

Comparison of Cassava Distillery Residues and Straw as Bulking Agents for Full-scale Sewage Sludge Composting

To solve problems of bulking agents shortage for sewage sludge (SS) composting and of disposal of cassava distillery residues (CDR) in the alcohol industry, the effects of using CDR instead of maize straw as bulking agents were investigated at full scale in Rizhao, China. This was done by comparing CDR-added and straw-added piles. Both CDR and straw promoted sanitation achievement of SS composts. CDR was effective at increasing fermentation temperature, whereas straw was superior in maintaining the thermophilic phase. The free air space (FAS) of matrices provided by CDR or straw were sufficient (>30%) for fermentation, and showed rising trends during composting. Extensive mass reduction was also accomplished in composting even after considering the weight of added straw or CDR. Compared with straw-added piles, CDR-added piles showed less average FAS (51.42%), water removal (49.68%), and organic matter (OM) loss (21.51%), but greater volume reduction (16.34%). The time-dependence of OM loss varied between the CDR-added (which followed zero-order kinetics) and straw-added (first-order kinetics) piles. After fermentation, the composts were phytotoxin-free in both treatments. The CDR-added piles had lower EC (2,221 μS cm^?1) and C/N (13.29) than straw-added piles. A smaller germination index (73.59%) for CDR-added piles, combined with higher pH (7.33) and oxygen consumption rate (4.33 mL L^?1 min^?1), suggests that SS composting with CDR requires a longer time to reach maturity than with straw. However, CDR can be used instead of maize straw as a bulking agent for full-scale SS composting.     

Evaluation of maturity and stability parameters of composts prepared from farm wastes

A composting experiment was carried out to study changes in physical [color, odor, temperature, organic matter (OM) loss], chemical [C:N ratio, water-soluble organic carbon (C_w):organic N (N_org) ratio, NH₄ ⁺-N and NO₃ ^?-N, humic acid (HA):fulvic acid (FA) ratio, humification index (HI) and cation-exchange capacity (CEC):total organic carbon (TOC) ratio)] and biological [seed germination index (GI)] parameters to assess compost maturity and stability over a period of 150 days. Five composts were prepared using a mixture of different farm wastes with or without enrichment of N, rock phosphate (RP) and microorganism (MO) inoculation. All the composts appeared to change to a granular and dark grey color without foul odor, and attained a constant temperature with no measurable changes (ambient level) at 120 days of composting. Correlation analysis showed that the optimal values of the selected parameters for our experimental conditions are as follows: organic matter loss > 42%, C:N ratio < 15, HA:FA ratio > 1.9, HI > 30%, CEC:TOC ratio > 1.7 and C_w:N_org ratio < 0.55. Composts enriched with N + RP or N + RP + MO matured at 150 and 120 days, respectively, whereas composts without any enrichment or enrichment with N or RP + MO did not mature even at 150 days of composting.     

Transformations of carbon and nitrogen during composting of animal manure and shredded paper

Composts produced from animal manures and shredded paper were characterized in terms of their carbon (C) and nitrogen (N) forms and C mineralization. Total, water-soluble, acid-hydrolyzable and non-hydrolyzable C and N contents were determined on composts sampled on days 0, 11, 18, 26, 33, 40 and 59 after composting was initiated. Water-soluble and acid-hydrolyzable C and N decreased during composting, whereas non-hydrolyzable C remained relatively constant, and non-hydrolyzable N greatly increased during composting. The water-soluble forms of N were characterized by a decrease of ammomium (NH₄ ⁺-N) at the beginning of composting, followed by an increase of nitrate (NO₃ ^–-N) towards the end of composting. The mineralization of C in composted materials was generally higher at the beginning than at the end of composting, whereas no differences were observed for mineralization of C in non-hydrolyzable materials. The addition of N inhibited C mineralization in composts except in samples collected on days 40 and 59, while C mineralization was strongly stimulated by adding N to the non-hydrolyzable materials. The data suggest that the N forms in the non-hydrolyzable materials were chemically similar and not readily available to microbes, indicating that the C/N ratios often used to assess the biodegradability of organic matter and to develop compost formulations should be based on biologically available N and C and not on total N and C. Received: 12 May 1997     

Chemical and microbiological parameters for the characterisation of the stability and maturity of pruning waste compost

Composting of pruning waste, leaves and grass clippings was monitored by different parameters. A windrow composting pile, having the dimensions 2.5 m (height) x 30 m (length) was establish. The maturation of pruning waste compost was accompanied by a decline in NH₄ ⁺-N concentration, water soluble C (WSC) and an increase in NO₃ ^–-N content. Both organic matter (OM) content and total N (TN) losses during composting followed a first-order kinetic equation. These results were in agreement with the microbiological activity measured either by the CO₂ respiration or dehydrogenase (DH-ase) activity during the process. Statistically significant correlations were found between DH-ase activity, easily biodegradable organic C forms, NH₄ ⁺-N and NO₃ ^–-N concentrations and organic matter content and N losses. For this reason, DH-ase activity and the CO₂ evolution could be used as good indicators of pruning waste compost maturity. In contrast, humification parameters data from the organic matter fractionation did not agree with the initially expected values and did not contribute to the assessment of compost maturity. Neither the cation exchange capacity nor the germination index showed a clear tendency during the composting time, suggesting that these parameters are not suitable for evaluating the dynamics of the process.     

Composting Olive Mill Waste and Sheep Manure For Orchard Use

An industrial-scale composting plant has been designed for producing organic fertilizers from olive mill waste using the windrow pile system. Materials to be composted, two phase olive mill waste (TPOMW) and sheep litter (SL), were characterized and made into three piles consisting of different proportions of each. Throughout the composting process, temperature (T), moisture (M), organic matter (OM), total organic carbon (C_org), total nitrogen (NT), germination index (GI), pH and electrical conductivity (EC) were monitored. The potential agronomic value of the final composts was ascertained by analyzing the bulk density, OM and Corg concentration, pH, EC, macro and micronutrient content (N, P, K, Ca, Mg, Fe, Cu, Mn, Zn, B), the concentration of humic and fulvic acids and inorganic nitrogen (NH₄⁺,NO₂^?,NO₃^?). Each compost was applied to an area of one hectare within a six year-old olive plantation. Four months after application, the soils showed an increased OM concentration and cationic exchange capacity (CEC).     

Relating Compost Measures of Stability And Maturity to Plant Growth

Assessment of compost maturity is important for successful use of composts in agricultural and horticultural production. We assessed the “maturity” of four different sawdust-based composts. We composted sawdust with either cannery waste (CW), duck manure (DM), dairy (heifer) manure (HM) or potato culls (PC) for approximately one year. Windrows were turned weekly for the first 60 days of composting, covered for four winter months and then turned monthly for six more months. We measured compost microbial respiration (CO₂ loss), total C and N, C:N ratio, water soluble NO₃-N and NH₄-N, dissolved organic carbon (DOC), pH and electrical conductivity at selected dates over 370 days. Compost effects on ryegrass biomass and N uptake were evaluated in a greenhouse study. We related compost variables to ryegrass growth and N uptake using regression analysis. All composts maintained high respiration rates during the first 60 days of composting. Ammonium-N concentrations declined within the first 60 days of composting, while NO₃-N concentrations did not increase until 200+ days. After 250+ days, DM and PC composts produced significantly more ryegrass biomass than either CW or HM composts. Total C, microbial respiration and water-extractable NO₃-N were good predictors of compost stability/maturity, or compost resistance to change, while dissolved organic carbon, C:N ratio and EC were not. The compost NO₃-N/CO₂-C ratio was calculated as a parameter reflecting the increase in net N mineralization and the decrease in respiration rate. At ratio values >8 mg NO₃-N/mg CO₂-C/day, ryegrass growth and N uptake were at their maximum for three of the four composts, suggesting the ratio has potential as a useful index of compost maturity.     

Characteristics of Composts Derived from Waxed Corrugated Cardboard

The characteristics of 12 composts containing, by volume, spent mushroom substrate (SMS, 50 percent), waste waxed corrugated cardboard (WCC, 0 percent, 25 percent or 50 percent), and/or pulverized wood wastes (WW, 50 percent, 25 percent or 0 percent) were measured during two separate windrow composting periods (12-16 weeks). Supplemental N was added to some of the composts in the form of poultry manure, and/or soybean processing wastes. During the first eight to 10 weeks, composts containing 50 percent WCC tended to reach and maintain the highest temperatures, but subsequently cooled most rapidly. Microbial activity (CO₂ evolution) also was initially highest in these composts but fell by the twelfth week to levels comparable to composts containing lower levels of WCC. The paraffin wax in WCC containing composts was almost completely degraded (>95 percent). After 12 weeks of composting N (1.2-1.6 percent DW), P (0.30-0.55 percent), and K (0.9-1.2 percent) concentrations were within typical ranges and N and P were highest in composts containing 50 percent WCC. KC1 extractable NH₄-N (494 mg-N kg^?1) and NO₃+NO₂-N (281 mg-N kg^?1) were highest and lowest, respectively, in composts containing 50 percent WCC. Electrical conductivity (4.5-8.5mS/cm) and pH (7.5-8.5) were high in all composts and highest in composts with 50 percent WCC. Concentrations of phenolic compounds were highest in composts containing 50 percent WCC, manure, and soybean wastes and were positively correlated with NH₄-N. C:N ratios of all composts were within an acceptable range (18-23:1).     

Optimization of Medlar Pruning Waste Composting Process by Cattle Manure Addition

In this study, medlar pruning waste (MPW) was composted with and without cattle manure (CM). Two piles were prepared: one contained only MPW (pile 1) and one contained MPW augmented with CM (pile 2). Both piles were composted in an enclosed composting vessel with passive aeration and aeration by turning. During the composting process, temperature, pH, electrical conductivity (EC), organic matter (OM), OM losses, total organic carbon (C_org), total nitrogen (N_T), C_org/N_T ratio, and germination index (GI) were measured. Pile 2 produced a faster increase of the temperature and had a longer thermophilic phase than pile 1. However, the rate of OM degradation was faster in pile 1 than in the pile containing CM (pile 2). The addition of CM also resulted in an increased pH and salt content. In both piles, C/N ratio decreased throughout the process, presumably as a result of the faster organic carbon degradation compared to N mineralization. However, only pile 2 had a final C/N ratio <20, the limit accepted for compost by the Spanish legislation on fertilizer. Also, both composts had GI > 50 percent, indicating an absence of phytotoxicity.     

Evaluation of Parameters During Composting of Two Contrasting Raw Materials

The aims of this work were: i) to evaluate, during a composting process, some parameters in two contrasting raw materials: one a ligneous material (C1) and the other (C2) a mixture of horse and poultry manure with a low straw percentage and ii) to compare results from microbiological and chemical analyses of both composting material during the process. Total carbon, total nitrogen, C: N ratio, ash, organic matter, organic matter destroyed, CEC, soluble organic carbon, soluble ammonium and nitrate, ammonium: nitrate ratio and respiration rate were evaluated during 18 weeks. C1 material showed a lower rate of organic matter mineralization probably due to the high proportion of ligneous material. This material reached a greater CEC during the experiment. Increase in CEC during composting is due to conversion of the remaining organic material into humic substances. These results would imply that C1 presented a greater humification level and consequently, a better quality. On the other hand, the greater decrease in soluble organic carbon and NH₄⁺-N values in C2 is in accordance with greater organic matter mineralization. A high decrease in soluble fractions, especially the more degradable ones (water soluble components) indicates a high mineralization of the organic matter during composting and a lower humification level. According to the data obtained in our experiment, some parameters such as CEC, soluble organic carbon and soluble NH₄⁺-N seem to achieve the stability level for both studied materials, while those parameters or indices such as C: N ratio, NH₄⁺-N: NO₃^?-N ratio indicated stability/maturity only in C2 material during the experimental time.     

Nitrogen and Phosphorus Availability in Groundfish Waste and Chitin-Sludge Cocomposts

Seafood processing generates a substantial volume of wastes. This study examined the feasibility of converting the fish waste into useful fertilizer by composting. Groundfish waste and chitin sludge generated from the production of chitin were composted with red alder or a mixture of western hemlock and Douglas-fir sawdust to produce four composts: alder with groundfish waste (AGF); hemlock/fir with groundfish waste (HGF); alder with chitin sludge (ACS); and hemlock/fir with chitin sludge (HCS). The resulting AGF had a higher total N and a lower C:N ratio than the other three composts. A large portion of the total N in the AGF, HGF, and HCS composts was in inorganic forms (NH₄⁺-N and NO₃^?-N), as opposed to only two percent in the ACS compost. Alder sawdust is more quickly decomposed, which favored N retention and limited nitrification during the composting period. It was less favorable than the hemlock/Douglas fir sawdust for composting with chitin sludge. Corn growth on soil amended with compost was dependent upon both compost type and rate. Nitrogen and P availabilities in all composts except the ACS were high and compost addition enhanced corn yields, tissue N and P concentrations, and N and P up-take. Neither the total N concentration nor the C:N ratio of the composts was an effective measure of compost N availability in the soil. Because soil inorganic N test levels correlated well with the corn biomass, tissue N and N uptake, they should be an effective measure of the overall compost effects on soil N availability and corn growth response. Phosphorus concentration, which increased linearly with increasing compost rates, was related to soil P availability from compost additions and correlated well with corn biomass, tissue P concentration and P uptake under uniform treatments of N and K fertilizers. Composting groundfish waste with alder or hemlock/Douglas-fir sawdust can produce composts with sufficient amounts of available N and P to promote plant growth and is considered to be a viable approach for recycling and utilizing groundfish waste.     

Nitrogen Availability from Compost in High Tunnel Tomato Production

High yield agricultural systems, such as high tunnel (HT) vegetable production, require a large supply of soil nutrients, especially nitrogen (N). Compost is a common amendment used by HT growers both to supply nutrients and to improve physical and biological soil properties. We examined commercially-available composts and their effects on soil N, plant N uptake, and tomato yield in HT cultivation. In addition, a laboratory study examined N and carbon (C) mineralization from the composts, and the usefulness of compost properties as predictors of compost N mineralization was assessed under field and laboratory conditions. The field study used a randomized complete block design with four replications to compare four compost treatments (all added at the rate of 300 kg total N ha^?1) with unamended soil and an inorganic N treatment (110 kg N ha^?1). Tomatoes were grown in Monmouth, Maine during the summers of 2013 and 2014. Compost NO₃^?-N and NH₄⁺-N application rates were significantly correlated with soil NO₃^?-N and NH₄⁺-N concentrations throughout the growing season. Marketable yield was positively correlated with compost total inorganic N and NO₃^?-N in both years, and with NH₄⁺-N in 2014. There were no significant differences among composts in percentage of organic N mineralized and no correlations were observed with any measured compost property. In the laboratory study, all compost-amended soils had relatively high rates of CO₂ release for the initial few days and then the rates declined. The compost-amended soils mineralized 4%–6% of the compost organic N. This study suggested compost inorganic N content controls N availability to plants in the first year after compost application.     

灌溉施用氮肥后氮素在土壤中的转化及淋失状况: 土柱模拟研究

A soil column method was used to compare the effect of drip fertigation (the application of fertilizer through drip irrigation systems, DFI) on the leaching loss and transformation of urea-N in soil with that of surface fertilization combined with flood irrigation (SFI), and to study the leaching loss and transformation of three kinds of nitrogen fertilizers (nitrate fertilizer, ammonium fertilizer, and urea fertilizer) in two contrasting soils after the fertigation. In comparison to SFI, DFI decreased leaching loss of urea-N from the soil and increased the mineral N (NH₄⁺-N + NO₃^--N) in the soil. The N leached from a clay loam soil ranged from 5.7% to 9.6% of the total N added as fertilizer, whereas for a sandy loam soil they ranged between 16.2% and 30.4%. Leaching losses of mineral N were higher when nitrate fertilizer was used compared to urea or ammonium fertilizer. Compared to the control (without urea addition), on the first day when soils were fertigated with urea, there were increases in NH₄⁺-N in the soils. This confirmed the rapid hydrolysis of urea in soil during fertigation. NH₄⁺-N in soils reached a peak about 5 days after fertigation, and due to nitrification it began to decrease at day 10. After applying NH₄⁺-N fertilizer and urea and during the incubation period, the mineral nitrogen in the soil decreased. This may be related to the occurrence of NH₄⁺-N fixation or volatilization in the soil during the fertigation process.     

Effect of Adding Flue Gas Desulphurization Gypsum on the Transformation and Fate of Nitrogen during Composting

The objective of this study was to investigate the effect of adding flue gas desulphurization gypsum (FGDG) on the transformation and fate of nitrogen during co-composting of dairy manure and pressmud of a sugar refinery. The ammonia absorption of FGDG was investigated. The changes in compost temperature, pH, electrical conductivity (EC), moisture, organic matter, the C/N ratio, Kjeldahl N, NH₄⁺-N, NO₂^?-N, NO₃^?-N were assessed. The addition of FGDG did not significantly affect compost temperature, pH, EC, moisture, and organic matter degradation. However, the addition of FGDG significantly increased the NH₄⁺-N content in the compost during the thermophilic phase, and the NH₄⁺-N maximal content in the compost with FGDG (CP+G) was 59.9% more than that in the compost without FGDG (CP–G). FGDG was thought to create the formation of (NH₄)₂SO₄ and the cation exchange between NH₄⁺ and Ca²⁺. The NO₂^?-N content in the CP+G peaked on day 15, and was not observed in the CP–G. In the final compost products, the NO₃^?-N concentration in the CP–G was more than that in the CP+G, which was 1451 (CP–G) and 1109 mg·kg^?1 (CP+G) dry material. This might be due to the NO₂^? accumulation in the CP+G, which accelerated N loss in the form of N₂O. There is a strong correlation between N₂O emission and NO₂^?-N accumulation in the composting process. Compared with the original N content in the compost mixture, the N loss in CP–G and CP+G were 15.0 and 10.8%, respectively. These results revealed that NH₄⁺-N conservation effect was improved during the thermophilic phase and the total N loss was mitigated by adding FGDG into composting materials. FGDG could be utilized as a potential amendment to conserve nitrogen during composting.     

Effect of pH on ammonium adsorption by natural Zeolite clinoptilolite

Abstract

Clinoptilolite, a zeolite mineral with a high cation exchange capacity and surface area, has ion‐exchange properties that can be utilized to adsorb NH₄ ⁺, protecting it from losses during composting of N‐rich animal manures. Ammonium adsorption by the natural zeolite clinoptilolite was studied to ascertain the effectiveness of the zeolite as an NH₄ ⁺ adsorbent at pH 4, 5, 6, and 7. The NH₄ ⁺ adsorption data were fitted to the one‐ and two‐surface Langmuir, Freundlich, and Temkin isotherms. All models described the NH₄ ⁺adsorption data successfully (r²≥0.939). The one‐surface Langmuir, Freundlich, and Temkin were converted to pH‐dependent forms. The amount of NH₄ ⁺ adsorbed increased as pH and initial NH₄ ⁺concentration increased. From the one‐surface Langmuir isotherm, the NH₄ ⁺adsorption capacity (X_m) of the zeolite increased linearly with pH (r²=0.994), and was estimated to be 9,660 mg N kg^‐1 at pH4, 11,220 mg N kg^‐1 at pH 5, 12,720 mg N kg^‐1 at pH 6, and 13,830 mg N kg^‐1 at pH 7. The adsorption of higher amounts of NH₄ ⁺with increasing pH and initial NH₄ ⁺concentration is an important characteristic of the zeolite that can be beneficial to minimizing N‐losses via NH₃volatilization during composting of N‐rich animal manures.     

Carbon and nitrogen transformation during composting of sweet sorghum bagasse

Two types of compost, consisting of sweet sorghum bagasse with either sewage sludge or a mixture of pig slurry and poultry manure, were studied in a pilot plant using the Rutgers system. The total degradation of the piles as determined by the weight loss of organic matter during the bio-oxidative and maturation phases accounted for 64% of the organic matter applied and followed a first-order kinetic function. Concentrations of total and organic N increased during the composting process as the degradation of organic C compounds reduced the compost weight. Losses of N through NH₃ volatilization were low, particularly in the compost with sewage sludge due to pH values of <7.0 and the low temperatures reached in the compost during the first 2 weeks. The C:N ratio in the two composts decreased from 24.0 and 15.4 to values between 12 and 10. Increases in cation exchange capacity and in fulvic and humic acid-like C revealed that the organic matter had been humified during composting. The humification index, the C:N ratio, fulvic:humic acid-like C, and cation exchange capacity proved to be the most suitable parameters for assessing the maturity of these composts.     

Does the potential ammonium fixation of soils have an impact on the optimum nitrogen fertilizer rate?

Field experiments were conducted to determine the effect of nitrogen (N) fertilizer forms and doses on wheat (Triticum aestivum L.) on three soils differing in their ammonium (NH₄) fixation capacity [high = 161 mg fixed NH₄-N kg^?1 soil, medium = 31.5 mg fixed NH₄-N kg^?1 soil and no = nearly no fixed NH₄-N kg^?1 soil]. On high NH₄⁺ fixing soil, 80 kg N ha^?1 Urea+ ammonium nitrate [NH₄NO₃] or 240 kg N ha^?1 ammonium sulfate [(NH₄)₂SO₄]+(NH₄)₂SO₄, was required to obtain the maximum yield. Urea + NH₄NO₃ generally showed the highest significance in respect to the agronomic efficiency of N fertilizers. In the non NH₄⁺ fixing soil, 80 kg N ha^?1 urea+NH₄NO₃ was enough to obtain high grain yield. The agronomic efficiency of N fertilizers was generally higher in the non NH₄⁺ fixing soil than in the others. Grain protein was highly affected by NH₄⁺ fixation capacities and N doses. Harvest index was affected by the NH₄⁺ fixation capacity at the 1% significance level.     

Distribution and stability of organic forms of nitrogen in forest soil profiles in Tanzania

The organic C and total N in Tanzanian forest soil profiles decreased with the depth but the C:N ratio and pH tended to increase. Soil pH ranged from 6.5 in the surface horizon to 7.3 in sub-surface ones.Of the total N in the surface horizon, 69.3–85.6% was hydrolysable in boiling 6 n HCl and 14.4–30.7% was nonhydrolysable. The amounts, expressed as percentage of total soil N, of NH⁺₄-N, hexosamine-N, serine + threonine-N (hydroxy amino acid-N) and amino acid-N in the total hydrolysable-N fraction ranged between 10.8–21.4, 5.2–11.5, 4.6–11.3 and 18.6–31.2, respectively. The amount of identified-N ranged between 43.3 and 60.0%, and that of unidentified-N between 24.1 and 36.0%. Amino acid-N constituted the largest portion of the identified-N. Total, NH⁺₄, hexosamine, amino acid (in Olmotonyi forest profiles only) and identified N fractions generally tended to decrease with depth in the profile but nonhydrolysable-N increased. Hydroxy amino acid-N and unidentified-N followed no definite trend.During aerobic incubation of surface soil, the amounts of total hydrolysable-N, hexosamine-N and hydroxy amino acid-N decreased while those of NH⁺₄-N and nonhydrolysable-N increased. All the organic N fractions underwent transformation during incubation. The hexosamines and hydroxy amino acids were more unstable than the others; the former being more vulnerable than the latter.     

Mechanisms of fixation and release of ammonium in paddy soils after flooding II. Effect of transformation of nitrogen forms on ammonium fixation

An incubation experiment under aseptic and septic conditions using ¹⁵N-labelled NH₄⁺-N and NO₃^–-N, was carried out to study the effect of N transformations after flooding on NH₄⁺ fixation in a paddy soil from China. After flooding ammonification was favoured, providing NH₄⁺ for fixation by clay minerals. NH₄⁺ fixation was more pronounced under low redox potential (E_h) conditions. Close correlations existed between exchangeable NH₄⁺, E_h, and non-exchangeable NH₄⁺. Therefore, two major conditions for NH₄⁺ fixation induced by flooding in paddy soil were found, namely flooding promoted net production of NH₄⁺ due to the deamination of organic N and, in addition, decreased the E_h of the soil. A lower E_h was caused by reduction and dissolution of Fe oxide coating on the clay minerals' surfaces, eliminating the obstacles for NH₄⁺ diffusing into or out of the interlayers of clay minerals. A higher concentration of exchangeable NH₄⁺ from deamination of organic N would drive NH₄⁺ diffusing from the soil solution into the interlayers of clay minerals. ¹⁵N-labelled NO₃^– incorporated into the flooded soil was not reduced to NH₃. The addition of NO₃^– retarded the decrease in the soil E_h and, therefore, NH₄⁺ fixation.     

Does the Ammonium Fixation Capacity of Soils Have a Significant Effect on the Nitrogen Nutrition of Wheat?

Pot experiments were conducted on three soils differing in their ammonium (NH₄ ⁺) fixation capacity [high = 161 mg NH₄-nitrogen (N) kg^?1 soil; medium = 31.5 mg NH₄-N kg^?1 soil; and no = no NH₄-N was additionally fixed], and the effect of N fertilizer forms and doses on wheat (Triticum aestivum L.) was investigated. Grain yields responded to almost all forms of N fertilizer with 80, 160, and 240 kg N ha^?1 in the high, medium, and no NH₄ ⁺ fixing soil process, respectively. Agronomic efficiency of applied N fertilizers was significantly greater in the no NH₄ ⁺ fixing soil. Thousand grain weights (TGW) of wheat grown on the high and medium NH₄ ⁺ fixing soil decreased with increasing N. Grain protein increased with increasing NH₄ ⁺ fixation capacity. Nitrogen doses and the forms of N fertilizers affected grain protein at a significance level. The combination of urea + ammonium nitrate (NH₄NO₃) was most effective in increasing grain protein content.