长期施肥条件下黑土有机碳和氮的动态变化

以黑土地区海伦农田生态系统国家野外科学观测研究站的农田长期(1990～2004年)肥料定位试验土壤为试验材料,对不同施肥处理有机碳和氮进行了系统分析。结果表明,长期不施化肥(CK),土壤中有机碳、全氮、碱解氮及C/N呈现下降趋势;长期施用氮肥,土壤有机碳亏损速率较大,但土壤氮素变化较小,C/N保持相对稳定;长期施用磷肥,土壤有机碳含量下降速率较小,但全氮含量下降速率较大,因而C/N保持上升趋势;长期施用钾肥,土壤有机碳、氮及C/N变化趋势相同,但土壤有机碳含量下降速率较小,C/N增加较快。因此,通过调控化肥可以维持土壤的有机碳、全氮及碱解氮含量,保持土壤的持续生产力及提高作物产量。     

黑土颗粒有机碳和氮含量对有机肥剂量响应的定量关系

黑土是一种非常重要的耕种土壤,但是由于过度地开发利用和水土流失导致土壤有机质含量迅速下降,严重影响了耕地生产力和作物产量。为了能够快速恢复黑土肥力,利用海伦国家野外科学观测研究站内的长期定位试验,定量分析了黑土颗粒有机碳和氮含量对有机肥剂量的响应。田间试验开始于2001年,设置了4个施肥处理,分别为:1单施化肥(OM0);2低剂量有机肥与化肥配施(OM1);3中剂量有机肥与化肥配施(OM2);4高剂量有机肥与化肥配施(OM3)。在2011年播种前,采集各处理0~20 cm耕层土壤样品。应用有机碳物理分组方法,测定分析了土壤有机碳、氮及各组分的含量。研究结果表明长期不同剂量有机肥输入能够显著增加黑土总有机碳和全氮含量(P0.05),每增施1 t有机肥,土壤有机碳含量增加0.186 kg,土壤全氮含量增加0.02 kg,表明增加有机肥投入量是提高黑土有机碳含量的有效措施。有机肥的施用增加了土壤中粗颗粒和细颗粒组分,不同剂量有机肥处理表现为OM3OM2OM1OM0,而减小了土壤中矿质结合态组分的含量;随着有机肥施入量的增加,粗颗粒和细颗粒土壤有机碳和氮的含量呈增加的趋势,而矿质结合态中的有机碳含量则略有下降,表明粗颗粒和细颗粒有机碳和氮是黑土有机碳和氮的主要储存库,有机无机配施对土壤有机碳、氮的提升作用主要集中于对活性组分颗粒有机质的形成和积累。与OM0处理相比,有机肥的施入显著降低了颗粒有机质和矿质结合态有机质的C/N,并且随着有机肥施入量的增加而逐渐降低。与单施化肥相比,化肥有机肥配施能够显著增加土壤的总有机碳,全氮,颗粒有机碳、氮含量,其中以化肥配施高剂量有机肥效果最佳,有利于黑土土壤肥力的快速提升,改善黑土的土壤质量。     

长期不同施肥对红壤碳氮储量的影响

利用20年长期肥料定位实验,研究了不同施肥条件下土壤全氮在0~20 cm、20~40 m、40~60 cm层次中分布特征。结果表明,不同施肥处理土壤全氮含量、C/N比值均以0~20 cm土壤层次中最高,随着土壤层次加深而出现下降。长期不同施肥处理后土壤全氮储量高低趋势为:有机肥料区组>化学肥料平衡施用区组>化学肥料不平衡施用区组,有机肥料和有机无机肥料配合施用效果最为显著。长期不同施肥,土壤碳氮比(C/N)无显著差异,长期施用有机肥料,土壤有机碳和土壤全氮数量同步上升,土壤C/N比值保持稳定。有机肥料与化学肥料配合施用能够提高土壤碳氮库容,是维持土壤肥力的最优施肥方式。     

长期施用化肥和秸秆对活性有机质组分的影响

以长期定位试验为基础,分析了长期施用化肥和秸秆对土壤不同组分C、N的影响.结果表明:连续施肥16年后,与CK处理相比,单施化肥使土壤全N、有机C、轻组有机N、颗粒态有机N、碱解N、易氧化有机C分别增加了13.8%,9.4%,38.2%,20.0%,19.7%和26.3%,但并没有增加轻组有机C量和颗粒态有机C量.化肥与秸秆配施后,土壤全N、有机C和不同组分C、N含量都显著大于单施化肥.单施化肥显著降低了土壤有机质、轻组有机质、颗粒态有机质的C/N比,但化肥与秸秆配施后, 其C/N比并没有继续降低.此外,易氧化有机C量与土壤有机C量显著相关,颗粒态有机N量、轻组有机N量、碱解N和土壤全N间也存在显著相关性.     

科尔沁沙地沙漠化过程中土壤碳氮特征分析

对科尔沁沙地不同沙漠化阶段土壤有机碳、全氮含量、土壤质地以及碳氮密度进行了测定与分析。结果表明,沙漠化过程中不同阶段的特征体现在土壤碳、氮、颗粒组成的有规律变化,土壤碳、氮含量衰减,质地变粗;从潜在沙漠化阶段到严重沙漠化阶段,100 cm深土壤有机碳和全氮含量分别下降91.6%和85.8%,有机碳密度从4 877.9(C)g/m2下降到481.8(C)g/m2,全氮密度从518.0(N)g/m2下降到89.4(N)g/m2;土壤有机碳、全氮与土壤细颗粒组分(<0.1mm)含量之间表现为同增同减性;沙漠化初期有机碳、氮衰减速率要快于沙漠化后期;沙漠化过程中土壤C/N比呈递减趋势,土壤有机碳衰减速率要快于全氮。     

长期施肥对黑土团聚化作用及碳、氮含量的影响

利用田间定位试验,研究了长期施用有机肥和化肥对黑土团聚化作用及碳、氮含量的影响。团聚体分级结果表明,小团聚体是土壤的主要组成部分,占土壤重量的48.86%~60.24%。长期施用有机肥可显著增加土壤中大团聚体的比例,提高土壤团聚体的稳定性;而化肥的施用则增加了土壤中微团聚体和粉+黏粒的含量,降低土壤团聚体的稳定性。施用有机肥能显著增加土壤有机碳和总氮的含量,而化肥只有与有机肥配施时才能显著增加土壤有机碳和总氮的含量。有机肥的施用能够降低土壤C/N,表明有机肥的施用更有利于土壤氮素水平的提高。     

不同施肥处理对棕壤几个肥力指标的影响

23年定位试验结果表明:土壤有机碳、全氮和碱解氮、生物氮量以:高量有机肥区>低量有机肥区>化肥区>对照。土壤C/N基本维持在11~16之间。单施化肥处理土壤微生物碳量降低,Kos值高于其它施化肥处理,有机无机肥配合施用,结合态腐殖质含量明显下降,松结态/重组腐殖质的比值和松/紧比值也有所降低。有机无机配施各处理土壤微生物碳量,以及土壤松结态、稳结态、紧结态腐殖质的含量显著升高,提高松结态/重组腐殖质和松/紧的比值;不同用量的施有机肥处理土壤微生物碳量与对照相比差异不显著,Kos值下降,其他指标均升高。     

长期施肥对棕壤有机碳组分的影响

对起始于1979年的棕壤长期肥料定位试验田2005年的耕层土壤不同有机碳组分进行了测定与分析,以探讨长期施肥影响土壤有机碳的过程及机理。结果显示:长期单施化肥降低了土壤的游离态颗粒有机碳（FPOM-C）含量,但进一步稳定了矿物结合态有机碳(MOM-C),最终提高了土壤总有机碳(TOC)含量;长期施用有机肥和有机肥配施化肥使土壤的FPOM-C、闭蓄态颗粒有机碳(OPOC)、MOM-C以及含量均显著提高,且增加效果好于单施化肥。从各组分有机碳所占比例或相对比值来看,长期施用有机肥和有机肥配施化肥提高了POM-C/TOC比例而降低了MOM-C/TOC比例,使FPOM-C/OPOM-C比值显著增大。表明土壤有机碳结构分组的应用有助于揭示长期施肥影响土壤有机碳的机理。     

长期施用生物有机肥对土壤肥力及微生物生物量碳的影响

为了研究施用生物有机肥(EM堆肥,即有效微生物制剂 堆肥)对土壤肥力及微生物生物量碳的影响,进行了7年的施用15t/hm2.a有机肥、施用7.5t/hm2.a有机肥(包括EM堆肥、EM鸡粪肥和传统有机肥)、施用化肥和对照处理的田间试验。结果表明:长期施用生物有机肥的土壤肥力明显提高。随着生物有机肥用量的提高,碱性土壤的pH值逐渐降低,土壤有机质、全N、碱解氮、有效磷、速效钾、微生物生物量碳含量增加,与有机肥施用量呈显著正相关。施用化肥可一定程度提高土壤有机质、全N和有效养分含量,但作用不明显。施肥对土壤肥力和微生物量碳的影响趋势是:EM堆肥>传统堆肥>化肥>对照。土壤微生物量碳与土壤有机质、全N、碱解氮、有效磷、速效钾含量呈显著正相关,可以作为施肥过程中土壤质量变化的生物学指标。     

长期施肥对土壤有机碳和无机碳的影响

利用18年长期定位试验,研究了在不同施肥条件下,土壤有机碳和无机碳在0~50 cm土层分布特征。结果表明,施肥对土壤有机碳的影响随着土层深度的增加而下降,0~7.5 cm土层的土壤有机碳比7.5~15 cm、15~30 cm、30~50 cm分别增加了4.6%、22.0%、63.1%,而无机碳含量随着土层深度的增加而增加,与有机碳的变化规律正好相反。不同种类的肥料对土壤有机碳的影响也不相同,化肥、有机肥长期配合施用和长期施用有机肥可以在0~30 cm土层增加土壤有机碳含量,降低土壤中的无机碳含量,而长期单施化肥对土壤的有机碳和无机碳含量无明显差异。     

连续施肥20年后棕壤团聚体分布和碳储量变化

利用两种不同分离方法(干筛法与湿筛法)对耕作施肥20年后棕壤的团聚体组成、团聚体有机碳含量以及有机碳储量进行了研究。结果表明:棕壤团聚体以0.25～1mm团聚体为主。与长期不施肥比较,除0.25～1mm粒级外长期施用氮磷化肥使风干团聚体和水稳性团聚体中较大团聚体和微团聚体数量下降,降低了各级风干团聚体中有机碳积累,增加了水稳性团聚体中有机碳积累;长期施用有机肥较大团聚体和微团聚体数量增加及其相连的有机碳含量和储量均增加;长期有机无机肥配施大团聚体数量下降,微团聚体数量增加,有机碳含量均增加,大团聚体碳库储量下降,微团聚体碳库储量增加。由此可见长期施有机肥土壤结构改善,固C潜力增加。长期高量有机肥与无机肥配施可能有利于土壤固碳,但不利于作物生长。     

中亚热带4种森林类型土壤有机碳氮贮量及分布特征

对湖南省长沙县大山冲省级森林公园立地条件基本一致的4种森林土壤有机碳(SOC)、全氮(N)含量、贮量及土层分布进行研究。结果表明:不同森林、同一森林的不同土层SOC、N含量和贮量均存在显著差异(p<0.05)。各土层SOC含量的变化范围分别为杉木林8.66～22.96g/kg,马尾松林13.33～37.50g/kg,南酸枣林13.78～43.58g/kg,青冈林13.33～36.87g/kg。土壤全N含量的变化范围分别为杉木林1.37～2.29g/kg,马尾松林1.29～2.65g/kg,南酸枣林1.69～3.96g/kg,青冈林1.23～3.26g/kg。4种林地SOC和N含量均随土层深度增加而逐渐下降。4种森林土壤SOC贮量差异不显著(p=0.177 6),N贮量差异极显著(p=0.000 7)。4种森林土壤SOC含量与N含量存在极显著相关性(p<0.01),除青冈林外,其余3种森林土壤SOC与C/N存在极显著相关关系(p<0.01)。马尾松土壤N与C/N之间呈极显著相关(p<0.01),南酸枣林的存在显著关系(p<0.05),青冈林和杉木林的相关性不显著。     

有机肥对棕壤不同粒级有机碳和氮的影响

采集棕壤长期肥料定位试验站不施肥和施用不同用量有机肥的土壤,通过超声波分散—离心分离得到细黏粒(<0.2μm)、粗黏粒(0.2～2μm)、粉粒(2～53μm)、细砂粒(53～250μm)和粗砂粒(250～2000μm)5个颗粒级别后,分析全土及不同粒级中土壤有机碳和氮并进行含量与分布的比较。结果表明,有机质主要分布于黏粒级中,其含量占全土有机碳的42.8%、全氮的58.3%,碳氮比随着粒级的增加而逐渐增大,表明氮易于在小粒级中富集。长期施用有机肥后,全土及各粒级有机碳和氮含量均有显著增加;砂粒级中有机碳和氮的富集系数升高,黏粒级中富集系数降低,粉粒级和砂粒级中的碳氮比降低。增加有机肥的用量加强了全土和各粒级对有机碳和氮的积累,同时加强了粉粒级和砂粒级碳氮比降低的程度。     

Dynamics of soil carbon to nitrogen ratio changes under long‐term fertilizer addition in wheat‐corn double cropping systems of China

Soil carbon to nitrogen (C:N) ratio is one of the important properties of terrestrial ecosystems. Here, we report a study of soil C:N ratio dynamics in wheat‐corn double cropping systems based on four long‐term experimental sites in China: three in the temperate zone and one in the sub‐tropical zone. We evaluate effects of long‐term fertilizer input on soil organic carbon (SOC) and total nitrogen (TN) by comparing three treatments: no added fertilizer (the control), added nitrogen‐phosphorus‐potassium chemical fertilizers (NPK), and chemical fertilizers combined with manure (NPKM). Our study shows that SOC and TN had different responses to the treatments. There was an increasing trend in SOC, even without fertilizer. However, applying inorganic fertilizers only (NPK) did not maintain TN contents at some sites. The NPKM treatment resulted in a large increase in both SOC (35–147%) and TN (33 to 10%) contents, relative to the initial values. The soil C:N ratio showed a significant increase over time at the sub‐tropical site but little change at the three temperate sites. Our analysis showed similar C:N ratios (37–38) in gross input of organic materials under the NPK treatments. However, the estimated C:N ratio during decomposition was much smaller at the sub‐tropical site (23.7) than at the three temperate sites (44.0–48.2) under the NPK treatments, which may explain the increased soil C:N ratio at the sub‐tropical site. Thus, we conclude that variations in soil C:N ratio are not caused by organic matter inputs but by decomposition in the wheat‐corn double cropping systems.     

Specific response of fungal and bacterial residues to one-season tillage and repeated slurry application in a permanent grassland soil

The dynamics of fungal and bacterial residues to a one-season tillage event in combination with manure application in a grassland soil are unknown. The objectives of this study were (1) to assess the effects of one-season tillage event in two field trials on the stocks of microbial biomass, fungal biomass, microbial residues, soil organic C (SOC) and total N in comparison with permanent grassland; (2) to determine the effects of repeated manure application to restore negative tillage effects on soil microbial biomass and residues. One trial was started 2 years before sampling and the other 5 years before sampling. Mouldboard ploughing decreased the stocks of SOC, total N, microbial biomass C, and microbial residues (muramic acid and glucosamine), but increased those of the fungal biomarker ergosterol in both trials. Slurry application increased stocks of SOC and total N only in the short-term, whereas the stocks of microbial biomass C, ergosterol and microbial residues were generally increased in both trials, especially in combination with tillage. The ergosterol to microbial biomass C ratio was increased by tillage, and decreased by slurry application in both trials. The fungal C to bacterial C ratio was generally decreased by these two treatments. The metabolic quotient qCO₂ showed a significant negative linear relationship with the microbial biomass C to SOC ratio and a significant positive relationship with the soil C/N ratio. The ergosterol to microbial biomass C ratio revealed a significant positive linear relationship with the fungal C to bacterial C ratio, but a negative one with the SOC content. Our results suggest that slurry application in grassland soil may promote SOC storage without increasing the role of saprotrophic fungi in soil organic matter dynamics relative to that of bacteria.     

Impact of activated charcoal and tannin amendments on microbial biomass and residues in an irrigated sandy soil under arid subtropical conditions

Effects of goat manure application combined with charcoal and tannins, added as feed additives or mixed directly, on microbial biomass, microbial residues and soil organic matter were tested in a 2-year field trial on a sandy soil under Omani irrigated subtropical conditions. Soil microbial biomass C revealed the fastest response to manure application, followed by microbial residue C, estimated on the basis of fungal glucosamine and bacterial muramic acid, and finally soil organic C (SOC), showing the slowest, but still significant response. At the end of the trial, microbial biomass C reached 220 μg g^?1 soil, i.e. contents similar to sandy soils in temperate humid climate, and showed a relatively high contribution of saprotrophic fungi, as indicated by an average ergosterol to microbial biomass C ratio of 0.35 % in the manure treatments. The mean fungal C to bacterial C ratio was 0.55, indicating bacterial dominance of microbial residues. This fraction contributed relatively low concentrations of between 20 and 35 % to SOC. Charcoal added to manure increased the SOC content and the soil C/N ratio, but did not affect any of the soil microbial properties analysed. Tannins added to manure reduce the 0.5 M K₂SO₄-extractable N to N total ratio compared to manure control. These effects occurred regardless of whether charcoal or tannins were supplied as feed additive or directly mixed to the manure.     

黄土高原不同年限刺槐土壤化学计量特征分析

通过对陕北黄土高原4种不同年限(10,15,25,40年)刺槐林地0—200cm土壤的采集与分析,研究不同刺槐种植时间对土壤营养元素及化学计量比的影响。在每个林龄的林地内设3个小区,每个小区采用随机采样法选取3个采样点分层采集土壤样品,进行土壤碳、氮、磷、钾测定,计算化学计量比。结果表明:(1)有机碳和全氮、全钾含量随刺槐年限增加呈基本增大的趋势,全磷随刺槐年限的增长变化不大;有机碳、全氮和全磷呈极显著的正相关关系;(2)在0—200cm土层,有机碳、全氮空间分布基本一致,随土层深度增加均呈先减少后趋于稳定的变化趋势;全磷在整个空间中的分布较为均匀,其空间变异性低于有机碳和全氮;全钾含量高于碳氮磷含量,且随土壤深度变化不大;(3)土壤C∶N比,C∶P比,N∶P比和C∶N∶P比均随刺槐年限的增长呈先降低后升高的趋势,都在15年处有最低值;(4)土壤C∶N比随土层深度在一定范围波动,C∶P和N∶P比均随土层深度的增加呈先减少后趋于稳定的变化趋势。     

Effect of Fertilizer Nitrogen (N) on Soil Organic Carbon,Total N,and Soil pH in Long-Term Continuous Winter Wheat (Triticum Aestivum L.)

Carbon sequestration via sound agronomic practices can assist in combating global warming. Three long-term experiments (Experiment 502, Experiment 222, and The Magruder Plots) were used to evaluate the effect of fertilizer nitrogen (N) application on soil organic carbon (SOC), total nitrogen (TN), and pH in continuous winter wheat. Soil samples (0–15 cm) were obtained after harvest in 2014, analyzed, and compared to soil test results from these experiments in 1993. Soil pH decreased with increasing N fertilization, and more so at high rates. Nitrogen application significantly increased TN in Experiment 502 from 1993 to 2014, and TN tended to be high at high N rates. Fertilizer N significantly increased SOC, especially when N rates exceeded 90 kg ha^?1. The highest SOC (13.1 g kg^?1) occurred when 134 kg N ha^?1 was applied annually. Long-term N application at high rates increased TN and SOC in the surface soil.     

Effects of tillage, organic resources and nitrogen fertiliser on soil carbon dynamics and crop nitrogen uptake in semi-arid West Africa

Tillage, organic resources and fertiliser effects on soil carbon (C) dynamics were investigated in 2000 and 2001 in Burkina Faso (West Africa). A split plot design with four replications was laid-out on a loamy-sand Ferric Lixisol with till and no-till as main treatments and fertiliser types as sub-treatments. Soil was fractionated physically into coarse (0.250–2 mm), medium (0.053–0.250 mm) and fine fractions (< 0.053 mm). Particulate organic carbon (POC) accounted for 47–53% of total soil organic carbon (SOC) concentration and particulate organic nitrogen (PON) for 30–37% of total soil nitrogen concentration. The POC decreased from 53% of total SOC in 2000 to 47% of total SOC in 2001. Tillage increased the contribution of POC to SOC. No-till led to the lowest loss in SOC in the fine fraction compared to tilled plots. Well-decomposed compost and single urea application in tilled as well as in no-till plots induced loss in POC. Crop N uptake was enhanced in tilled plots and may be up to 226 kg N ha⁻¹ against a maximum of 146 kg N ha⁻¹ in no-till plots. Combining crop residues and urea enhanced incorporation of new organic matter in the coarse fraction and the reduction of soil carbon mineralisation from the fine fraction. The PON and crop N uptake are strongly correlated in both till and no-till plots. Mineral-associated N is more correlated to N uptake by crop in tilled than in no-till plots. Combining recalcitrant organic resources and nitrogen fertiliser is the best option for sustaining crop production and reducing soil carbon decline in the more stabilised soil fraction in the semi-arid West Africa.