保护性耕作对潮土团聚体组成及其有机碳含量的影响

利用保护性耕作长期定位试验平台,研究了小麦-玉米轮作条件下连续5年实施免耕与秸秆还田对农田潮土团聚体组成、团聚体中有机碳含量的影响。结果表明:相比于常规翻耕,常规翻耕+秸秆还田、免耕、免耕+秸秆还田处理下5 mm粒级机械稳定性团聚体含量显著提升,增加比例分别为16.62%、16.05%、44.23%;而免耕、免耕+秸秆还田能显著提升5~2 mm粒级水稳性团聚体含量,提升比例分别为29.81%和64.28%,同时团聚体稳定率也有一定的提高;实施免耕能显著提高5~2、2~1 mm粒级水稳性团聚体中有机碳含量;除常规翻耕+秸秆还田处理下2~1 mm粒级外,秸秆还田对各粒级团聚体中有机碳含量均有不同程度的提升作用。     

抛荒对冷浸稻田土壤团聚体及有机碳稳定性的影响

冷浸稻田是长江流域重要的低产稻田类型之一,近年来抛荒严重,而抛荒对冷浸稻田土壤团聚体的影响并不清楚。本研究以连年种植的冷浸稻田(CWC)、抛荒3年的冷浸稻田(CWA3)和抛荒6年的冷浸稻田(CWA6)为对象,分析抛荒后冷浸稻田土壤团聚体特征以及有机碳稳定性,以期为准确评估抛荒对长期淹水土壤的结构和有机碳的影响提供数据支持。结果表明,不论是0~25 cm土层还是25~50 cm土层,冷浸稻田土壤53μm粒级团聚体占总团聚体比例均超过40%;0~25 cm土层土壤250μm团聚体比例超过35%;53~250μm粒级团聚体比例低于20%。抛荒使0~25 cm土层53μm粒级团聚体占总团聚体比例显著增加,53~250μm粒级比例显著降低。在0~25 cm土层,抛荒使有机碳活性指数Ⅰ(LIc-Ⅰ)在53μm粒级和250μm粒级上升高,有机碳活性指数Ⅱ(LIc-Ⅱ)在53~250μm和250μm粒级上降低;而有机碳难降解指数(RIc)在53μm和53~250μm粒级上降低。土壤总有机碳随抛荒时间延长而增加。     

长期保护性耕作提高土壤大团聚体含量及团聚体有机碳的作用

【目的】团聚体形成被认为是土壤固碳的最重要机制。本文以河南豫西地区长期耕作试验为研究对象,研究了长期保护性耕作对土壤团聚体性质及土壤有机碳(SOC)含量的影响,为探讨土壤固碳机理,优化黄土高原坡耕地区农田耕作管理措施,实现土壤固碳减排、培肥土壤提供理论依据。【方法】长期耕作试验开始于1999年,试验处理有免耕覆盖(NT)、深松覆盖(SM)和翻耕(CT)。利用湿筛法筛分第3年(2002年)和第13年(2011年)0—10cm和10—20 cm土层中,2000、250~2000、53~250和53μm级别的水稳性团聚体,计算团聚体平均质量直径(MWD),并测定了各级别团聚体的有机碳(SOC)含量。【结果】1)连续13年免耕覆盖和深松覆盖显著提高了土壤表层0—10 cm的SOC含量,分别比翻耕增加了33.47%和44.48%。2011年免耕覆盖和深松覆盖SOC含量较2002年上升了1.92%和8.59%,翻耕下降了18.97%。2)与翻耕相比,免耕覆盖和深松覆盖2000μm团聚体含量显著提高了40.71%和106.75%;53~250μm团聚体含量显著降低了19.72%和22.53%;团聚体平均质量直径显著提高了20.55%和39.68%,显示了土壤结构的明显改善。3)免耕覆盖和深松覆盖显著提高了表层土壤所有团聚体有机碳的含量,尤其以2000μm团聚体提升最多。与翻耕相比,2000μm团聚体有机碳分别提高了40.0%和27.6%。4)免耕覆盖和深松覆盖下表层土壤大团聚体有机碳含量随耕作年限增加,微团聚体有机碳随耕作年限降低。2000μm的土壤团聚体有机碳含量2011年较2002年分别升高了23.93%和7.12%,53~250μm微团聚体有机碳含量分别下降了19.58%和13.27%。【结论】长期保护性耕作(包括免耕覆盖和深松覆盖)可显著提高表层土壤大团聚体含量,降低微团聚体含量,提高团聚体的水稳性,改善土壤结构。同时可增加土壤团聚体有机碳含量,提高土壤肥力。长期保护性耕作在河南豫西丘陵地区是一种较为合理的耕作方式。     

黄土丘陵区不同植被群落土壤团聚体有机碳及其组分的分布

有机碳是形成土壤团聚体的重要物质,植被群落通过有机残体的输入增加土壤有机碳含量,从而通过影响团聚体的形成而影响土壤结构。为探究不同植被群落对土壤结构改良的意义,对黄土丘陵区森林带和草原带的不同植被群落土壤团聚体中有机碳组分进行了研究。结果表明:(1)研究区域森林带土壤有机碳含量大于草原带,森林带植被群落土壤总有机碳含量大小顺序为:辽东栎群落>人工刺槐群落>狼牙刺群落,草原带植被群落土壤总有机碳含量大小顺序为:人工沙棘群落>达乌里胡枝子+茭蒿群落>铁杆蒿+达乌里胡枝子群落;(2)土壤活性有机碳和腐殖质碳占土壤总有机碳的比例在两种植被带之间基本相同,相同植被群落土壤活性有机碳占土壤总有机碳的比例高于腐殖质碳占总有机碳的比例;(3)森林带土壤>0.25 mm团聚体含量显著高于草原带土壤>0.25 mm团聚体含量,各种形态的有机碳随着土壤团聚体粒级的增大有机碳含量呈先增加后减少或者随着团聚体粒级的增大而增大的趋势,2~0.25 mm和<0.25 mm团聚体中有机碳含量最高;(4)草原带每种植被群落土壤活性有机碳含量空间差异性较大,辽东栎群落各种形态土壤有机碳含量的空间差异性都较大,<0.25 mm团聚体腐殖质碳含量大于其他粒径;(5)草原带人工沙棘群落土壤各种形态有机碳在土壤剖面上的含量差异很小,其他各植被群落0~10 cm土层土壤有机碳含量均大于10~20 cm土层。     

黄土丘陵区植被恢复中不同粒级土壤团聚体有机碳分布特征

对黄土丘陵区土壤有机碳在不同粒级团聚体中的分布特征及其对植被恢复的响应进行了研究。结果表明：（1）黄土丘陵区不同植被覆盖条件下,土壤有机碳的分布具有一定的表聚性,0～20 cm土层中有机碳的含量均高于20～40 cm中有机碳的含量,不同植被群落下有机碳的含量大小为：大针茅群落〉长芒草群落〉铁杆蒿群落〉百里香群落;（2）同一深度土壤各粒级团聚体中有机碳的分布特征是：0.5～0.25 mm与1～0.5 mm两个粒级中有机碳的含量最高,〉1 mm的团聚体中有机碳的含量有随粒级增大而减小的趋势;（3）恢复年限对不同粒级土壤团聚体中有机碳的含量影响很大,有机碳的含量随恢复年限的增加总体呈上升趋势。黄土高原沟壑区土壤有机碳的积累与土壤团聚体的粒级和植被恢复的类型、年限等有明显的关系。     

有机无机肥长期施用对潮土团聚体及其有机碳含量的影响

用湿筛法和密度分级相结合的方法,将土壤区分为不同粒径团聚体,并进一步把团聚体细分成不同组分,系统比较了有机无机肥长期施用对潮土及其团聚体中有机C含量的影响。结果表明,潮土团聚体以53～250μm为主,有机肥长期施用显著提高了250～2000μm团聚体数量,降低了53～250μm团聚体的比例。无机肥施用增加了各粒径团聚体有机C含量,但没有改变团聚体的比例。团聚体中有机C以细颗粒有机C(fine iPOM)为主,有机肥施用显著增加了矿物态有机C含量,并通过增加250～2000μm团聚体中细颗粒有机C(fine iPOM)和矿物态有机C比例来提高土壤有机C含量。无机肥则通过比较均匀地增加团聚体中各组分有机C含量来提高土壤有机C水平。因此,有机肥和无机肥在潮土上增加土壤有机质含量的机制存在明显差异。     

黑土区落叶松人工林土壤团聚体有机碳及动态变化

以东北典型黑土区林龄分别为8a,21a,30a,37a,52a的落叶松人工林为研究对象,通过对土壤干团聚体组成、不同粒级团聚体有机碳含量及有机碳贮量的测定、计算与分析,研究了不同林龄落叶松人工林土壤团聚体有机碳分配特征及其动态变化规律。结果表明:在0-30cm土层范围内,各粒级团聚体组成比例随粒级从大到小呈"∧"型分布,2～5mm粒级团聚体所占比例均最大,且与其他粒级存在显著性差异(P0.05)。表层土壤各粒级团聚体有机碳含量均大于下层土壤,表层土壤中各粒级团聚体有机碳平均变化范围为32.17～48.75g/kg,下层土壤中的变化范围28.82～33.53g/kg,并均以2～5mm粒级团聚体有机碳含量最低。团聚体有机碳贮量随林龄的变化表现为大粒级团聚体(5～10mm)有机碳贮量的降低和小粒级有机碳贮量的增加。表层土壤有机碳贮量大于下层土壤有机碳贮量,但均以2～5mm粒级团聚体有机碳贮量最高,故可以认为土壤有机碳贮量的变化主要取决于各个粒级团聚体组成比例而不是各个粒级团聚体有碳含量。研究结果为进一步评价人工林植被恢复对土壤有机碳的影响提供了理论依据和参考。     

不同有机厩肥输入量对土壤团聚体有机碳组分的影响

土壤是重要的有机碳库,其微小变化可能引起大气CO2浓度水平的较大变异。土壤团聚体对土壤有机碳具有物理保护作用。有机厩肥的输入既可以提高土壤有机碳含量,又可以促进土壤团聚体的形成,对土壤有机碳的截获和保持有重要意义。本实验采用湿筛的方法分离土壤团聚体,并对团聚体进行有机碳组分分离。通过对连续8年施加不同量有机厩肥试验的研究发现:适量的有机厩肥施用可以显著地提高土壤的平均质量直径(MWD),改善土壤结构;过量施用有机厩肥则明显降低了>2 000μm团聚体含量。潮棕壤有机碳主要分布在250～53μm和2 000～250μm团聚体中,两者相加约占有机碳全量的73.7%～78.5%。并且随着有机碳输入量的增加,土壤有机碳主要贮存在2 000～250μm团聚体中。有机厩肥的施加明显地加快了>2 000μm团聚体的更新速率。土壤轻组分有机碳含量也随有机厩肥输入量的增加而不断增加,高量有机厩肥下占全量的22.1%。土壤固定有机碳的能力有限,存在明显的等级饱和现象。因此,在有机质匮乏的土壤施用有机肥意义重大,应尽量减少向高有机质土壤输入有机碳。     

改良剂作用下滨海盐化潮土团聚体分布、稳定性及有机碳分布特征

通过对滨海盐化潮土小麦—玉米轮作2年田间定位试验,研究不同改良剂施用对土壤团聚体分布、稳定性及土壤团聚体中有机碳含量、各级团聚体有机碳对总有机碳贡献率的影响。试验共设置3个处理:对照(CK)、有机土壤改良剂(OSA)和有机—无机土壤改良剂(CSA),分析土壤团聚体分布、水稳性大团聚体(R_(0.25))、平均重量直径(mean weight diameter,MWD)、几何平均直径(geometric mean diameter,GMD)、分形维数(D)、有机碳储量(soil organic carbon storage,SOCS)和有机碳贡献率(contribution rate of organic carbon)。结果表明,滨海盐化潮土水稳性团聚体组成主要以0.25 mm粒径为主,改良剂施用后土壤R_(0.25)显著提高,其影响主要集中在5 mm和2～5 mm粒径级,OSA处理2个粒级团聚体含量较CK分别显著增加167.38%和59.00%,CSA处理分别显著增加89.17%和100.66%。施用OSA与CSA同时显著提高了土壤团聚体MWD和GMD值,说明2种改良剂的施用均有利于提高大团聚体数量及稳定性。施用改良剂2年处理土壤各粒级团聚体中有机碳含量均有所提高,OSA处理以1～2 mm粒级提高最多,CSA以2～5 mm粒级提高最多,且前者达显著水平。与CK相比,改良剂可促使土壤有机碳向大团聚体富集,显著提高1～2 mm粒级团聚体对土壤总有机碳的贡献率93.62%～109.76%,降低或显著降低1～2 mm粒级团聚体对土壤总有机碳的贡献率20.55%～24.92%。在小麦—玉米轮作模式下,改良剂施用不仅可以显著提高滨海盐化潮土水稳性大团聚体含量和稳定性,还可显著增加水稳性大团聚体有机碳含量与储量,是加强盐碱土壤有机碳库积累的有效措施。     

不同施肥制度下褐土微团聚体有机碳活性变化

通过8年田间定位试验,研究了不同施肥制度下褐土及其微团聚体有机碳活性变化规律。结果表明,褐土微团聚体有机碳活性随粒级的减小而增强,不同施肥制度下土壤微团聚体组成变化各异,不施肥主要是由于10~50μm粒级总有机碳和<10μm粒级易氧化有机碳储量减少而造成土壤有机碳活性降低,土壤肥力下降;单施常量NPK化肥处理呈同样的趋势;增量NPK化肥可保持有机碳活性和肥力不降低,但投入较高;有机肥(物)料配施常量NPK化肥主要是通过增加大粒级特别是10~50μm粒级总有机碳和易氧化有机碳储量而使有机碳活性和土壤肥力水平得到提高。采用有机肥(物)料配施常量NPK化肥是改善褐土肥力的有效措施。     

耕作方式对土壤水分入渗、有机碳含量及土壤结构的影响

为探明不同耕作方式对土壤剖面结构、水分入渗过程等的作用机理,采集田间长期定位耕作措施(常规耕作、免耕、深松)试验中的原状土柱(0~100 cm)及0~10 cm、10~20 cm、…、90~100 cm环刀样、原状土及混合土样,通过室内模拟试验进行了0~100 cm土层土壤入渗过程和饱和导水率的测定,分析了不同土层的土壤有机碳含量、土壤结构特征及相互关系。结果表明:从土柱顶部开始供水(恒定水头)到水分全部入渗到土柱底部的时间为:常规耕作免耕深松;土柱土壤入渗速率和累积入渗量为:深松免耕常规耕作;土柱累积蒸发量为:常规耕作免耕深松。土壤的饱和导水率表现为:0~10 cm和50~60 cm土层,免耕深松常规耕作;20~50 cm和60~100 cm土层,深松免耕常规耕作。随土层的加深,0.25 mm水稳性团聚体含量和土壤有机碳含量均表现为先增加(10~20 cm)再降低的趋势。在0~40 cm土层和80~100 cm土层,均以深松处理0.25 mm水稳性团聚体含量最高。在60 cm以上土层,土壤有机碳含量表现为:免耕深松常规耕作,而60 cm土层以下土壤有机碳显著降低,均低于4 g·kg?1,且在70 cm以下土层,常规耕作免耕深松。综上,耕作措施能够改变土壤有机碳含量,改善土壤结构,促进土壤蓄水保墒;深松更利于水分就地入渗,而免耕则更利于有机碳的提升和水分的储存,其作用深度在0~60 cm土层。     

免耕对土壤团聚体特征以及有机碳储量的影响

以实施7年的中国科学院禹城综合试验站冬小麦夏玉米轮作免耕长期定位试验场为对象,研究免耕条件下土壤水稳性团聚体和有机碳储量的变化,为进一步评价免耕措施对黄淮海平原土壤结构和质量的影响提供科学依据。设置免耕(NT)、免耕秸秆不还田(NTRR)、常规耕作(CT)3种处理,分析土壤表层(0~20 cm)及深层(20~60 cm)水稳性团聚体分布特征、土壤有机碳以及团聚体有机碳的变化和相互关系。研究结果表明:由于减少了对土壤的破坏以及增加了秸秆还田和有机肥的施用,与常规耕作相比,NT和NTRR可提高表层土壤有机碳含量和储量、水稳性团聚体平均重量直径(MWD)和几何平均直径(GMD),以及大团聚体有机碳的含量和储量。其中,秸秆覆盖比施用有机肥对表层土壤有机碳储量和0.25~2 mm团聚体有机碳储量的提高具有更显著的作用。与表层不同,深层土壤有机碳和大团聚体有机碳的含量和储量表现为NT相似文献   

中国黄土高原区轮耕对土壤团聚体、有机碳氮含量的影响

In rain-fed semi-arid agroecosystems, continuous conventional tillage can cause serious problems in soil quality and crop production, whereas rotational tillage (no-tillage and subsoiling) could decrease soil bulk density, and increase soil aggregates and organic carbon in the 0-40 cm soil layer. A 3-year field study was conducted to determine the effect of tillage practices on soil organic carbon (SOC), total nitrogen (TN), water-stable aggregate size distribution and aggregate C and N sequestration from 0 to 40 cm soil in semi-arid areas of southern Ningxia. Three tillage treatments were tested: no-tillage in year 1, subsoiling in year 2, and no-tillage in year 3 (NT-ST-NT); subsoiling in year 1, no-tillage in year 2, and subsoiling in year 3 (ST-NT-ST); and conventional tillage over years 1-3 (CT). Mean values of soil bulk density in 0-40 cm under NT-ST-NT and ST-NT-ST were significantly decreased by 3.3% and 6.5%, respectively, compared with CT, while soil total porosity was greatly improved. Rotational tillage increased SOC, TN, and water-stable aggregates in the 0-40 cm soil, with the greatest effect under ST-NT-ST. In 0-20 and 20-40 cm soils, the tillage effect was confined to the 2-0.25 mm size fraction of soil aggregates, and rotational tillage treatments obtained significantly higher SOC and TN contents than conventional tillage. No significant differences were detected in SOC and TN contents in the >2 mm and <0.25 mm aggregates among all treatments. In conclusion, rotational tillage practices could significantly increase SOC and TN levels, due to a fundamental change in soil structure, and maintain agroecosystem sustainability in the Loess Plateau area of China.     

Soil carbon fractions and relationship to soil quality under different tillage and stubble management

Effect of 19 years of different tillage (direct drilled vs. conventional tillage) and stubble management (stubble retained vs. burnt) on soil carbon fractions were studied in a red earth, an Oxic Paleustalf at Wagga Wagga, NSW. The changes in carbon fractions were related to observed changes in soil structural stability and nitrogen availability. Significant differences in total organic carbon (TOC) were detected to 0.20 m depth, but the largest differences existed in the top 0.05 m where a difference of 8.0 g/kg (equivalent to 5.2 t ha⁻¹) was found between the extreme treatments (direct drilled/stubble retained (DD/SR) vs. conventional cultivation/stubble burnt (CC/SB)). Tillage had a much greater effect in reducing total carbon than stubble burning accounting for 80% of the total difference between the extreme treatments in 0–0.05 m layer. Tillage and stubble burning resulted in lower levels of different organic carbon fractions with tillage preferentially reducing the particulate organic carbon (POC) (>53 μm) (both free and associated POCs), whereas stubble burning reduced the incorporated organic carbon (<53 μm). We also found that tillage and stubble burning both significantly lowered the water stability of aggregate >2 mm, whereas stubble burning was related to the reduction of water stability of aggregates <50 μm. Furthermore, tillage was related to the decline in mineralisable nitrogen (MN) due to the loss of POC, especially the free POC fraction. POC was a more sensitive indicator of soil quality changes under different tillage and stubble management than TOC.     

No‐tillage with half‐amount residue retention enhances microbial functional diversity,enzyme activity and glomalin‐related soil protein content within soil aggregates

A 3‐year field tillage and residue management experiment established in North China was used to analyse topsoil (0–15 cm) aggregation, and microbial functional diversity, enzyme activity and glomalin‐related soil protein (GRSP) content within aggregates. Compared with conventional tillage (CT), no‐tillage (NT) alone significantly (P < 0.05) increased organic C contents in 50–250 and <2 μm aggregates and decreased the proportion of C accumulated by 2–50 μm aggregates and microbial functional diversity indices in <2 μm aggregates. Regardless of tillage practice, both half‐amount (C50) and full (C100) residue retention tended to increase organic C and GRSP contents, or dehydrogenase and invertase activities, in certain aggregates. Under CT, a poorer performance of C50 than C100 was observed in maintaining Shannon index (H′) and Simpson index (D) in >250 and <2 μm aggregates, and also McIntosh index (U) in <2 μm aggregates, owing to insufficient residue and possible decreases in the distribution of decomposer micro‐organisms. Under NT, however, C50 was more effective than C100 in maintaining/elevating H′, D and U in all soil aggregates except for 50–250 μm, suggesting that surplus residue may induce worse soil conditions, decreasing heterotrophic microbial activities. Thus, NT with half‐amount residue retention improved soil physical–chemical–biological properties and could be a useful management practice in North China.     

Contributions of soil biota to C sequestration varied with aggregate fractions under different tillage systems

It is increasingly believed that substantial soil organic carbon (SOC) can be sequestered in conservation tillage system by manipulating the functional groups of soil biota. Soil aggregates of different size provide diverse microhabitats for soil biota and consequently influence C sequestration. Our objective was to evaluate the contributions of soil biota induced by tillage systems to C sequestration among different aggregate size fractions. Soil microbial and nematode communities were examined within four aggregate fractions: large macroaggregates (>2 mm), macroaggregates (2–1 mm), small macroaggregates (1–0.25 mm) and microaggregates (<0.25 mm) isolated from three tillage systems: no tillage (NT), ridge tillage (RT) and conventional tillage (CT) in Northeast China. Soil microbial and nematode communities varied across both tillage systems and aggregate fractions. The activity and abundance of microbes and nematodes were generally higher under NT and RT than under CT. Among the four aggregate fractions, soil microbial biomass and diversity were higher in microaggregates, while soil nematode abundance and diversity were higher in large macroaggregates. Structural equation modelling (SEM) revealed that the linkage between microbial and nematode communities and their contributions to soil C accumulation in >1 mm aggregate fractions were different from those in <1 mm aggregate fractions. Higher abundance of arbuscular mycorrhizal fungi (AMF) could enhance C retention within >1 mm aggregates, while more gram-positive bacteria and plant-parasitic nematodes might increase C accumulation within <1 mm aggregates. Our findings suggested that the increase in microbial biomass and nematode abundance and the alteration in their community composition at the micro-niche within aggregates could contribute to the higher C sequestration in conservation tillage systems (NT and RT).     

Impact of tillage and crop rotation on light fraction and intra-aggregate soil organic matter in two Oxisols

It is well known that no-tillage (NT) practices can promote greater stocks of soil organic matter (SOM) in the soil surface layer compared to conventional tillage (CT) by enhancing the physical protection of aggregate-associated C in temperate soils. However, this link between tillage, aggregation and SOM is less well established for tropical soils, such as Oxisols. The objective of this study was to investigate the underlying mechanisms of SOM stabilization in Oxisols as affected by different crop rotations and tillage regimes at two sites in southern Brazil. Soils were sampled from two agricultural experiment sites (Passo Fundo and Londrina) in southern Brazil, with treatments comparing different crop rotations under NT and CT management, and a reference soil under native vegetation (NV). Free light fraction (LF) and intra-aggregate particulate organic matter (iPOM) were isolated from slaking-resistant aggregates. Of the total C associated with aggregates, 79–90% was found in the mineral fraction, but there were no differences between NT and CT. In contrast, tillage drastically decreased LF-C concentrations in the 0–5 cm depth layer at both sites. In the same depth layer of NT systems at Londrina, the concentrations of iPOM-C were greater when a legume cover crop was included in the rotation. At Londrina, the order of total iPOM-C levels was generally NV > NT > CT in the 0–5 cm depth interval, but the difference between NT and CT was much less than in Passo Fundo. At Passo Fundo, the greatest concentrations and differences in concentrations across tillage treatments were found in the fine (53–250 μm) iPOM fractions occluded within microaggregates. In conclusion, even though no aggregate hierarchy exists in these Oxisols, our results corroborate the concept of a stabilization of POM-C within microaggregates in no-tillage systems, especially when green manures are included in the rotation.