施用硒、锌、铁对玉米和大豆产量与营养

采用黄土高原低硒区典型土壤,以玉米、大豆为供试作物,研究施用硒、锌、铁对作物产量和营养品质的影响。结果表明,仅施用氮、磷、钾肥料,玉米、大豆产量无明显变化,施用适量硒、锌、铁时,玉米产量明显提高15.6%～73.8%,大豆产量提高58.9%～115.1%。施用硒、锌、铁,玉米籽粒氮含量明显增加,而磷、钾含量无明显变化,大豆籽粒氮含量无明显变化,磷、钾含量有所下降。土施或结合叶面喷施硒、锌、铁时,玉米和大豆籽粒硒、锌、铁含量明显增加,尤其是土施结合叶面喷施硒、锌、铁时效果突出。与对照比较,玉米籽粒硒增加480.4%、锌增加106.9%、铁增加126.7%; 大豆籽粒硒增加512.9%、锌增加62.2%、铁增加15.6%。综合考虑玉米、大豆产量和籽粒硒、锌、铁含量变化,锌、硒适宜土壤施用,或土施结合叶面喷施施用,叶面喷施铁可有效提高作物籽粒铁含量。     

叶面喷硒对水稻籽粒硒富集及分布的影响

在大田试验条件下，研究了江苏省苏南地区水稻（龙晴4号、86优8和丙9652）叶面喷施和未喷施亚硒酸钠对水稻籽粒硒含量、硒的形态及对蛋白质结合态硒的影响。结果表明：叶面喷施亚硒酸钠10g hm^-2，可使该地区水稻籽粒硒含量达255．3—586．5μg kg^-1，是不喷硒的7．9-11．0倍。无论喷硒还是未喷硒，水稻籽粒硒主要以有机硒的形态存在，有机硒含量占总硒量的87．72％-90．98％，对产量没有显著差异。总体看来，叶面喷硒可以显著提高籽粒蛋白结合态硒含量，籽粒蛋白质结合的硒占有机硒的43．07％-51．65％，占总硒的38．04％-48．98％。叶面喷硒与对照相比，二个品种（龙晴4号与丙9652）籽粒的蛋白谱带基本一致，只是喷硒的水稻籽粒在14～20kDa之间增加了一些蛋白条带，这可能是由于喷硒增加了一些小分子蛋白质的合成。     

不同施硒方式对水稻硒利用效率的影响

采用盆栽试验方法,研究土壤施硒和叶面喷硒两种施硒方式(均为硒酸钠形态)对水稻籽粒硒生物强化以及营养品质的影响机制。结果表明:等量施硒条件下,土壤施硒和叶面施硒两种方式均能显著提高水稻地上部和籽粒硒含量,土壤施硒水稻地上部和籽粒硒含量分别为叶面喷硒处理的8.9倍和5.3倍,说明在等量施硒条件下土壤施硒较叶面施硒更能有效提高籽粒硒含量。叶面喷硒处理的籽粒硒分配系数为土壤施硒的2倍,说明硒通过叶片向籽粒转运速率较通过根系到籽粒的转运速率更快。土壤施硒能够显著提高籽粒中镁、硫、铁、锰和锌等矿质营养元素含量。相比对照组,土壤施硒和叶面喷硒的水稻籽粒粗蛋白分别提高8%和4.5%,丝氨酸和酪氨酸含量说明施硒能够提高水稻营养品质,以土壤施硒效果更佳。     

叶面喷施硒对水稻籽粒中硒含量及形态的影响

为研究叶面喷施氨基酸螯合硒对水稻籽粒硒含量及形态的影响,以水稻品种百香 139 为材料,通过大田试验,在水稻破口期叶面喷施不同用量的氨基酸螯合硒,并在水稻拔节孕穗期、始穗期、扬花授粉期、灌浆期、蜡熟期 5个不同生育期叶面喷施 13.5 g/hm²(以硒计)的氨基酸螯合硒,在水稻成熟期测定、分析各处理组水稻地上部不同组织器官硒含量和籽粒硒形态。结果表明,喷施外源氨基酸螯合硒可提高水稻各器官硒含量,在适量范围内水稻植株各器官硒含量随着喷硒浓度的增加而提高;水稻籽粒中硒主要以硒代蛋氨酸存在,水稻籽粒中硒代蛋氨酸含量与总硒含量的变化规律相一致。喷施氨基酸螯合硒用量为 6.75、13.5、27.0 g/hm² 时水稻籽粒中硒含量分别为 0.168、0.431、0.439 mg/kg,均达到《富硒农产品硒含量分类要求》(DB45/T 1061-2014)的要求。拔节孕穗期至灌浆期喷硒处理水稻籽粒硒含量呈现增高趋势,灌浆期喷硒处理的水稻籽粒总硒含量最高,达到 0.320 mg/kg;水稻籽粒中硒代蛋氨酸含量占总硒含量的比例为 43% ～ 91%,硒代蛋氨酸含量占总硒含量的比例由大到小依次为:拔节孕穗期 >始穗期>扬花授粉期＝灌浆期>蜡熟期,硒代蛋氨酸比例随着施硒时期越靠近水稻成熟期越低,表明水稻叶片中硒的转化时间对稻米中硒形态有显著影响,提早喷施硒有利于有机态硒的形成,提高稻米中有机态硒的比例。在硒的生物强化生产富硒水稻中,水稻破口期叶面喷施氨基酸螯合硒的最佳用量为 13.5 g/hm²,在 5 个不同生育期喷施 13.5 g/hm² 的氨基酸螯合硒均能生产出富硒大米,且最佳喷施外源硒的时期为灌浆期。     

叶面过量施硒对玉米产量、硒和矿质营养元素含量的影响

为了明确过量施硒对作物生长的毒害效应,为合理施用硒肥提供依据,本研究在田间条件下探究了叶面过量施硒对糯玉米京科糯2000和普通玉米郑单958两个品种产量、硒含量和矿质营养元素含量的影响。结果表明,叶面喷硒量(亚硒酸钠)≤0.2 g·L^-1时,京科糯2000植株生物量和籽粒产量与对照(不喷硒)相比持平或略有增加,未表现出毒害效应;京科糯2000在叶面喷硒量≥0.5 g·L^-1时,郑单958在叶面喷硒量≥0.1 g·L^-1时,其植株生物量和籽粒产量均明显降低;随着叶面喷硒浓度的增加,两品种玉米籽粒硒含量均逐步增加,郑单958产量和籽粒硒含量的变化幅度高于京科糯2000。叶面喷硒可显著提高玉米籽粒锌和铁的吸收量,对铜、磷、镁和钙的吸收基本无影响,但抑制硼的吸收。总体来看,过量施硒抑制2个不同品种玉米生长,造成产量下降,但可提高籽粒硒、锌和铁的营养水平。本研究对玉米微量元素营养调控具有一定的借鉴指导意义。     

叶面喷施硒肥对小麦籽粒及面粉硒含量的影响

为了探明叶面喷施硒肥对小麦籽粒及制粉各粉路硒含量的影响,本研究选用4个小麦品种,在拔节和抽穗期分2次喷施0.017%亚硒酸钠叶面肥,并对成熟期籽粒进行制粉,分析各粉路的总硒、无机硒和有机硒含量。结果表明,叶面喷硒对小麦籽粒和面粉的总硒、有机硒和无机硒含量的影响存在品种间差异。喷施硒肥后,4个小麦品种籽粒的总硒含量提高了26.4~35.8倍,但小麦籽粒产量无显著变化。皮磨粉、心磨粉、统粉、小麸和大麸5种粉路中,小麸的总硒含量最高,其次为大麸,皮磨粉、心磨粉和统粉的总硒含量较低。与硬质小麦相比,软质小麦出粉率低,面粉的总硒量损失较多。经制粉,对照和喷硒样本的总硒积累量分别损失52.97%~69.28%和36.73%~44.51%。对照样本中,各粉路的有机硒含量略高于无机硒含量;而喷硒处理样品中,除宁麦20的小麸外,各粉路的有机硒含量均略低于无机硒含量。本研究揭示了制粉粉路硒分布规律,这对于富硒小麦的加工利用具有重要的指导作用。     

叶面喷施硅和硒对水稻砷积累及光合参数的影响

本文采用盆栽试验，研究了在砷污染土壤上，水稻叶面分别喷施硅溶液、硒溶液和硅硒混合溶液对籽粒砷含量相对较低的水稻品种旌1优华珍和C两优华占以及籽粒砷含量相对较高的水稻品种丰优香占砷积累和光合参数的影响。试验结果表明，3种叶面喷施处理相比较而言，以叶面单独喷施硅降低水稻籽粒砷含量效果最好，优于叶面单施硒和叶面喷施硅硒混合液处理。叶面单独喷施硅与对照相比，显著降低了籽粒砷含量相对较低的两个水稻品种的籽粒砷含量，但对籽粒砷含量相对较高的水稻品种则没有显著影响。籽粒砷含量相对较低的水稻品种更多比例的砷固定在茎中使其稻米砷积累量较低。3种叶面喷施处理与对照相比均提高了水稻光合参数(如光合速率、气孔导度、胞间CO₂浓度)，光合参数与水稻砷浓度呈负相关关系，叶面喷施硅或硒对水稻叶片光合参数的影响差异可能是造成籽粒砷含量差异的重要原因，不同水稻品种叶片光合参数对外源硅或硒的敏感度或响应度不同。总之，叶面喷施硅溶液对改善水稻光合参数的效果最好，是减少水稻籽粒砷含量一项经济有效的措施，在生产应用实践中，要注意作物品种间的差异。     

不同时期叶面喷硒对冬枣含硒量与品质的影响

为了筛选冬枣(Zizyphus jujuba Mill. var. Dongzao)的最佳施硒方案,通过在展叶期、幼果期、果实膨大期、白熟期分别进行一次性叶面喷施亚硒酸钠溶液(含硒量为45 mg·L~(-1),施硒量为27 mg·株~(-1)),研究不同时期施硒对冬枣叶片和果实的硒含量、硒有机化程度以及果实品质的影响。结果表明:在果实膨大期或白熟期叶面喷施硒溶液后,叶片和果实的总硒含量均得到显著提高,其中果实膨大期喷硒使总硒和有机硒含量增幅最大,冬枣果实含硒量由25μg·kg~(-1)提高到76μg·kg~(-1)。不同时期叶面喷硒对冬枣果实品质的作用效果不尽相同,果实膨大期施硒对冬枣品质的改善效果最佳,此时冬枣果实中的可溶性固形物、可溶性糖、维生素C、总黄酮含量和糖酸比均达到最高值,分别较对照显著提高了13.30%、17.17%、16.33%、29.17%和28.89%。综合研究结果表明,生产富硒冬枣以在果实膨大期一次叶面喷硒的效果最佳。     

低硒土壤区玉米植株中硒的分布及调控初探

对低硒地区玉米植株中硒的含量分布规律进行了研究，采籽玉米成熟期根系＞茎叶雄穗（混）＞籽粒，籽粒中含硒量非常低；青贮玉米灌浆期叶片＞茎秆＞根系＞雄穗＞雌穗，下部茎＞上部茎，上部叶≈下部叶。用亚硒酸钠溶液浸种不利于提高玉米含硒量，叶喷可有效地提高玉米各器含硒量，其含硒量随喷硒量的增加而增加，喷１５－７５ｇ／ｈｍ＾２可使玉米茎叶含硒量达到充足阈，喷硒对玉米产量没有明显提高。     

浔郁平原不同作物的硒富集特征及其影响因素

作物中的硒含量是影响食物链硒水平的关键因素。以浔郁平原土地质量地球化学评价项目中的农作物调查结果和多年定位试验结果为基础,探究不同作物对土壤硒的富集特征及其影响因素。结果表明:浔郁平原的覃塘、港南、桂平市和平南县等4个县(市、区)的水稻富硒率均高于86%。花生、黄豆、玉米和水稻的天然富硒率最高,富硒率依次为100%、100%、100%和89%。水稻根、茎、籽粒的生物富集系数依次下降,分别为0.663、0.130和0.108,水稻籽粒的生物富集系数远高于龙眼、荔枝和甘蔗。根系土与水稻籽粒硒含量的相关系数为0.660,远高于龙眼和荔枝。港南区作物硒与土壤pH相关系数为0.342,在碱性土壤中甘蔗茎的硒含量(0.012 1 mg/kg)最高,在酸性土壤中水稻的硒含量(0.0653mg/kg)最高。本研究中,以种子为可食部分的作物其天然富硒率较高,土壤硒含量和土壤酸碱性对水稻籽粒硒含量影响较大。     

Effects of different application methods of selenite and selenate on selenium distribution within wheat

Abstract

The objective of this sand culture experiment was to determine how fertilization methods (i.e., fertigation rates of 0.5, 1, and 2?mg Se·pot^?1, foliar rates of 5, 10, and 20?mg Se· L^?1) and fertilizer type (i.e., selenate or selenite) affected wheat Se concentrations. The results showed that the fertigation and foliar treatments both increased wheat Se content. In the fertigation and foliar treatment total Se content of wheat was greatest in the selenate treatments. In the fertigation treatment, the Selenite had mainly accumulated in the roots, while the Selenate was majority transported to shoot. In the foliar treatment, we found that most of Se was transported to grain. In addition, the organic Se concentration was the most than other Se fractions. In conclusion, selenate was more effective than selenite in both the fertigated and the foliar application treatments. And the foliar application was better than fertigation.     

锌肥不同施用方式及施用量对我国主要粮食作物增产效果的影响

【目的】施用锌肥是改善作物缺锌、 提高产量和籽粒锌含量的重要措施。锌肥的施用效果受多种因素的影响,通过总结自70年代以来锌肥施用对我国主要粮食作物小麦、 玉米、 水稻产量的影响,分析不同年代、 锌肥施用方式、 锌肥用量对这三大作物产量影响的进程,探讨锌肥的适宜用量和施用方式。【方法】利用万方数据库、 中国知网,查阅了1970至2013年间,我国主要粮食作物水稻、 小麦和玉米锌肥施用相关的田间试验文献333篇,剔除文献中没有产量数据、 没有具体施肥相关信息如施肥量、 施肥方式等文献,有效样本数总计为1656个。采用相关分析、 方差分析等统计分析方法,Microsoft Excel 2010软件分析。【结果】锌肥增产效果受锌肥施用方式、 施用量、 年代的影响,具体结果如下, 1)锌肥施用方式土壤施用、 叶面喷施和种子处理在小麦上的平均增产率分别为11.3%、 10.0%和11.1%; 在玉米上的平均增产率分别为13.7%、 12.7%和12.1%; 水稻上的平均增产率分别为15.0%、 9.8%和9.7%。与叶面喷施和种子处理相比,无论是小麦、 玉米还是水稻,土施锌肥的增产效果最好。2)锌肥施用量小麦、 玉米和水稻的增产率随土施锌肥量增加而增加,当施锌量达到一定量后,随施肥用量的进一步增大,增产率有所降低。小麦、 玉米和水稻土施锌肥的合适用量分别为1545 kg /hm2、 2030 kg/hm2、 2030 kg/hm2。小麦增产率与喷施锌肥的浓度关系不明显,叶面喷施浓度在0.4%~0.5% ZnSO47H2O时增产效果最佳; 而玉米、 水稻增产率和叶面喷施锌肥的浓度变化趋势与土施锌肥变化趋势一致。过去40年玉米和水稻适宜喷施锌肥浓度分别是0.1%~0.3%、 0.2%~0.4% ZnSO47H2O。3)施肥年代随着年代的变化,不同作物施用锌肥的增产幅度不同。随着年代的推进,同一锌肥施用方式在小麦上增产率呈逐渐增高的趋势; 锌肥土施和叶面喷施在玉米上的增产率呈下降趋势; 锌肥土施在水稻上的增产率呈下降趋势,而叶面喷施在水稻的增产率呈先降低后增加的趋势; 种子处理方式在水稻和玉米上的增产率随年代的变化不明显。【结论】施用锌肥能有效提高小麦、 玉米和水稻的产量,但是其增产效果受锌肥施用方式、 施用量、 年代的影响。因此,今后在锌肥施用方面,农户应根据作物、 土壤、 环境等条件,选择恰当的施肥方式及锌肥用量,来提高锌肥的增产效果。     

Se肥施用方式和施用时期对水稻含Se量及产量的影响

在田间小区的试验条件下研究不同硒(Se)肥的施用方法及施用时期对水稻植株和籽粒中Se的吸收与富集的影响。研究表明:水稻在生长前期和旺盛时期对Se的吸收和积累有很大差异。与对照相比,苗床施Se、土壤施Se和叶面喷Se 3种方式均能明显提高各生育期水稻植株和籽粒中Se的含量,富Se效果达到差异极显著水平。从稻米富Se效果看,富Se含量在0.163~0.207 mg·kg-1之间,Se含量增加了5.8~7.6倍,且都在安全范围内。从水稻富Se的方式上,不同施Se方式富Se效果依次为水稻扬花期喷Se土壤施Se育苗期喷Se对照。从叶面喷施Se的时间上,稻米富Se效果依次是扬花期喷Se育苗期喷Se灌浆期喷Se齐穗期喷Se对照。从水稻富Se类型上看,外源无机Se和有机Se都能明显促进水稻对Se的积累。水稻育苗期和扬花期叶面喷Se在提高水稻稻米中含Se量的同时,并没有降低水稻产量。因此水稻叶面喷Se是一条安全、简单、易操作的途径。     

海藻多糖–纳米硒的结构及其促进水稻硒累积的机理

【目的】以海藻提取物和亚硒酸钠为原料,采用抗坏血酸还原法制备了海藻多糖–纳米硒(APS-SeNPs),以期为富硒大米生产提供高效的富硒肥料。【方法】采用单因素试验和响应面法优化APS-SeNPs的制备工艺;借助傅里叶红外光谱、透射电镜和表面张力仪对APS-SeNPs进行表征。通过开展大田试验,探究0、5、25mg/L APS-SeNPs叶面喷施对粳稻‘洛稻998’和籼稻‘川香优2号’硒累积的影响。【结果】1) APS-SeNPs的最佳制备工艺:硒添加量2800 mg/L,亚硒酸钠与抗坏血酸的摩尔比19∶100,反应温度32℃,时间20 min。APSSeNPs粒径为38.94 nm,Zeta电位为–52.17 mV。红外分析结果表明,海藻多糖与纳米硒形成了新的Se—O化学键。APS-SeNPs的表面张力为46.23 mN/m,显著低于Na₂SeO₃。APS-SeNPs在水稻叶片上的持液量为6.28mg/cm²,而Na₂SeO₃只有3.94 mg/cm²。2...     

Effect of zinc application methods on apparent utilization efficiency of zinc and potassium fertilizers under rice-wheat rotation

Apparent utilization of zinc (Zn) and potassium (K) fertilizers was examined in rice (Oryza sativa L.)-wheat (Triticum aestivum L.) using combinations of no K; soil applied K levels and no Zn; soil and foliar applied Zn. Application of 33.2 kg K ha^?1 in rice and 24.9 kg K ha^?1 in wheat along with foliar spray of 2 kg Zn ha^?1 at 30 and 60 days gave the highest mean grain yields. Foliar application of zinc increased Zn concentration in flag leaves, grain, and straw of rice and wheat and K concentration in flag leaves of rice and straw of wheat significantly. Potassium application increased Zn concentration in rice grain and straw and K concentration in wheat straw significantly. Zinc and K increased the uptake of each other in grain; straw and total uptake by both crops significantly. Zinc fertilizer enhanced the utilization of soil K. Potassium fertilizer enhanced the utilization of applied Zn.     

硒对水稻光合作用及抗氧化作用的影响

硒是植物非必需营养元素,但是适量的硒对植物生长有着促进作用。通过研究硒对水稻光合作用及抗氧化作用的影响为硒对水稻生长的促进作用提供依据。采用盆栽试验,研究不同施硒浓度(0~5 mg/kg)对水稻产量、硒含量、叶绿素含量、光合参数、抗氧化酶活性及细胞膜透性的影响。结果表明,施硒显著提高了水稻产量,在1.0和5.0 mg/kg施硒水平较对照(不施硒)分别增产20.8%和28.8%。施硒提高了水稻谷壳、大米、秸秆及根中硒的含量。施硒对水稻叶绿素a和叶绿素b含量影响不大,但在5.0 mg/kg施硒水平上显著增加了水稻类胡萝卜素含量。施硒显著增加了叶片胞间二氧化碳浓度、蒸腾速率和光合速率,最高增幅分别为28.4%、26.1%及25.0%。施硒显著降低了水稻叶片离子渗漏量及相对电导膜透性。施硒显著增加了超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性,最高增幅分别为101.2%、23.4%和64.3%。试验结果表明,硒能增强水稻的光合作用及抗氧化作用,从而达到了促进水稻生长的效果。     

Effect of zinc application methods on apparent utilization efficiency of zinc and phosphorus fertilizers under basmati rice–wheat rotation

To examine the effect of zinc (Zn) application method on the utilization of phosphorus (P) from applied P fertilizer, a field experiment was conducted on basmati rice–wheat rotation with combinations of Zn levels (0, soil application of 2.5 kg Zn ha ^{– 1} and two foliar applications of 2.0 kg Zn ha ^–1) and P levels (0, soil application of 8.7, 17.5 and 26.2 kg P ha ^–1). The highest pooled grain yields of basmati rice and wheat were obtained with soil application of 17.5 kg P ha ^–1 and foliar applications of 2 kg Zn ha ^–1. Foliar applications of Zn increased the P concentration in grain and straw and the total P uptake by basmati rice and the P concentration in flag leaves of wheat significantly, while soil or foliar application of Zn increased the total P uptake of wheat. Phosphorus application increased the Zn concentration in flag leaves, grain and straw of basmati rice and in grain and straw of wheat and the total Zn uptake of both crops. Phosphorus levels up to 17.5 kg P ha ^–1 increased utilization efficiency of soil or foliar application of Zn. Zinc application increased the P utilization efficiency of basmati rice and wheat up to 17.5 kg P ha ^–1 level; foliar Zn application was more effective in a wheat crop than a rice crop.     

Determination of selenium concentration of rice in china and effect of fertilization of selenite and selenate on selenium content of rice

A method of hydride generation atomic fluorescence spectrometry was applied to the determination of the selenium concentration of regular polished rice in China and selenium-enriched polished rice obtained by foliar application of selenium-enriched fertilizer in the forms of selenite and selenate. The average selenium content of regular rice was 0.025 +/- 0.011 microg g(-)(1). On the basis of a daily dietary rice intake of 300-500 g suggested by the China Nutrition Society, the total selenium intake from regular rice was calculated to be 7.5-12.5 microg per person per day for an adult. The selenium contents of rice were significantly increased to 0.471-0.640 microg g(-)(1) by foliar application of selenium-enriched fertilizer at rate of 20 g of Se ha(-)(1) in the forms of sodium selenite and sodium selenate. The selenium content of rice by application of a fertilizer of selenate was 35.9% higher than that by a fertilizer of selenite, which showed that Se-enriched fertilizer in selenate exhibited greater efficiency in increasing Se content in rice products. The Se-enriched rice products can increase daily Se intake on average by 100-200 microg of Se per day by the consumption of 400 g of rice products if the Se level of rice products is controlled at 0.3-0.5 microg of Se g(-)(1). Because rice is a staple food in China, selenium-enriched rice obtained by bioenrichment of selenium to increase the Se content of rice could be a good selenium source for the population in selenium-deficient regions.     

Long-Term Sulfur Fertilization: Effects on Crops and Residual Effects in a No-Till System of Argentinean Pampas

A long-term experiment has been conducted between 2001 and 2008 at Balcarce, Argentina, to determine the effect of sulfur (S) fertilization on S concentration in grains, crop yield, and residual S in soil. Two treatments were evaluated: annual S application to crops (15 kg ha^?1; S1) and a control with no S fertilization (S0). Sulfur fertilization only increased wheat yield (22% of the crops in the experiment). However, S application increased S concentration in grains in wheat, soybean, and maize (56% of the crops). Although, for all years, the S mass balance was positive for S1 and negative for S0, no differences in soil S extracted as sulfate (S-SO₄ ^?2) content previous to the crop sown were determined. The absence of differences in S accumulation in aboveground vegetative biomass and grain of the maize used as a check also suggest that long-term S fertilization did not affect the soil S availability for crops.     

Zinc and amino acids on wheat-soybean intercropping under no-till management

Abstract

Foliar fertilization with micronutrients and amino acids (AAs) has been used to increase the grain yield and quality of different crops. The aim of the present study was to evaluate the effects of Zn and AAs foliar application on physiological parameters, nutritional status, yield components and grain yield of wheat-soybean intercropping under a no-till management. We used a randomized block experimental design consisting of eight treatments and four replicates. The treatments were five Zn rates (0, 1, 2, 4 and 8?kg ha^?1) and 2?L ha^?1 of AAs and three additional treatments: a control (without the Zn or AA application), 2?kg ha^?1 Zn and 2?kg ha^?1 Zn + 1?L AA. The treatments were applied by spraying during the final elongation stage and at the beginning of pre-earing for the wheat and in growth stage V6 for the soybean for two crop years in a Typic Oxisol (860?g kg^?1 clay). Zinc foliar fertilization increased the wheat grain Zn concentrations. The Zn rates and AA foliar fertilization in soil with did not affect the physiological parameters, nutrient status or yield components. The AA application at the different concentrations tested changed the soybean grain yield and the leaf N concentration. The results suggest that Zn and amino acids application increases the grains Zn concentration in the wheat, being an important strategy to agronomic biofortification.