Influence of Nitrogen on Growth,Yield, and Yield Components and Nitrogen Uptake and Use Efficiency in Dry Bean Genotypes

Dry bean is an important legume and nitrogen (N) deficiency is one of the most yield-limiting factors in most of the bean-growing regions. A greenhouse experiment was conducted with the objective to determine influence of N on growth, yield, and yield components and N uptake and use efficiency of 23 dry bean genotypes. Straw yield, grain yield, yield components, maximum root length, and root dry weight were significantly increased with the addition of N but varied with genotypes. The N × genotype interactions were also significant for most of these traits, indicating variation in responses of genotypes with the variation in N levels. There was significant difference in N uptake and use efficiency among genotypes. Most of growth and yield components were significantly and positively associated with grain yield. Based on grain yield efficiency index (GYEI), genotypes were classified into efficient, moderately efficient, or inefficient group in N-use efficiency. Nitrogen concentration was greater in grain compared to straw, indicating greater N requirement of dry bean genotypes.     

Dry Bean Genotypes Evaluation for Zinc-Use Efficiency

Dry bean is an important legume for human consumption worldwide. Low soil fertility, including zinc (Zn) deficiency, is one of the main factors limiting yield of this legume in South America, including Brazil. The objective of this study was to evaluate 30 dry bean genotypes for zinc (Zn)–use efficiency. The Zn rates used were 0 mg Zn kg^?1 (low) and 20 mg Zn kg^?1 (high) of soil. Grain yield, straw yield, number of pods, hundred-seed weight, number of seeds per pod, maximum root length, and rood dry weight were significantly affected by Zn and genotype treatments. The Zn × genotype interactions were also significant for growth, yield, and yield components, indicating that some genotypes were highly responsive to the Zn application while others were not. Based on seed yield efficiency index (SYEI), genotypes were classified as efficient, moderately efficient, and inefficient in Zn-use efficiency. Most efficient genotypes were CNFP 10104, BRS Agreste, BRS 7762 Supreme, CNFC 10429, BRS Estilo, CNFC 10467, BRS Esplendor, and BRS Pitamaba. The most inefficient genotype was BRS Executive. Remaining genotypes were moderately efficient in Zn-use efficiency.     

Copper-Use Efficiency in Dry Bean Genotypes

Copper (Cu) is an essential micronutrients and its deficiency has been reported in many crops including dry bean. A greenhouse experiment was conducted to evaluate thirty dry bean genotypes (G) for Cu-use efficiency. The Cu levels used were low (natural soil level) and adequate [10 mg Cu kg^?1 soil, applied with copper sulfate (24 percent Cu)]. Straw yield, seed yield, number of pods per plant, seed per pod, seed harvest index (SHI), maximum root length (MRL), and root dry weight (RDW) were significantly affected by Cu and genotype treatments. The Cu × G interactions were also significant for these traits, indicating variation in genotype responses with the variation in Cu levels. Based on seed yield efficiency index (SYEI), genotypes were grouped in three classes: Cu efficient, moderately Cu efficient, and Cu inefficient. Fifty-three percent of the genotypes were classified as efficient, 40 percent were classified as moderately efficient, and 7 percent were classified as inefficient in Cu-use efficiency.     

Growth of Tropical Legume Cover Crops as Influenced by Nitrogen Fertilization and Rhizobia

Tropical legume cover crops are important components in cropping systems because of their role in improving soil quality. Information is limited on the influence of nitrogen (N) fertilization on growth of tropical legume cover crops grown on Oxisols. A greenhouse experiment was conducted to evaluate the influence of N fertilization with or without rhizobial inoculation on growth and shoot efficiency index of 10 important tropical cover crops. Nitrogen treatment were (i) 0 mg N kg^?1 (control or N₀), (ii) 0 mg N kg^?1 + inoculation with Bradyrhizobial strains (N₁), (iii) 100 mg N kg^?1 + inoculation with Bradyrhizobial strains (N₂), and (iv) 200 mg N kg^?1 of soil (N₃). The N?×?cover crops interactions were significant for shoot dry weight, root dry weight, maximal root length, and specific root length, indicating that cover crop performance varied with varying N rates and inoculation treatments. Shoot dry weight is considered an important growth trait in cover crops and, overall, maximal shoot dry weight was produced at 100 mg N kg^?1 + inoculation treatment. Based on shoot dry-weight efficiency index, cover crops were classified as efficient, moderately efficient, and inefficient in N-use efficiency. Overall, the efficient cover crops were lablab, gray velvet bean, jack bean, and black velvet bean and inefficient cover crops were pueraria, calopo, crotalaria, smooth crotalaria, and showy crotalaria. Pigeonpea was classified as moderately efficient in producing shoot dry weight.     

Zinc-Use Efficiency in Upland Rice Genotypes

Zinc (Zn) deficiency is very common in annual crops grown on Brazilian Oxisols. A greenhouse experiment was conducted to evaluate Zn-use efficiency of 20 upland rice genotypes. The Zn levels used were 0 mg kg^?1 (natural level of the soil) and 20 mg kg^?1 of soil applied with zinc sulfate (ZnSO₄). Zinc × genotype interactions were significant for grain yield, panicle number, panicle length, root dry weight, and specific root length, indicating different responses of genotypes with the variation of Zn levels and that selection for Zn-use efficiency is necessary at low as well as at high Zn rates. Based on Zn-use efficiency index, 11 genotypes were classified as efficient and nine were classified as moderately efficient. The most Zn-efficient genotypes were BRA 01596, BRA 042156, BRA 052053, BRA Primavera, and BRA 01506. The most inefficient genotypes in Zn-use efficiency were BRA 042094, BRA 052045, BRA 052034, and BRA 052023. Grain yield and most of the yield attributing characteristics have significant Zn × genotype interactions, which indicate that genotypes respond differently under different Zn levels. Thus, genotype selection is an important strategy for upland rice production in Brazilian Oxisols.     

Nutrient Uptake in Dry Bean Genotypes

Dry bean is an important legume for human consumption in South America. A greenhouse experiment was conducted to evaluate uptake and use efficiency of macro- and micronutrients by six dry bean genotypes at two P levels (25 and 200 mg kg^?1 soil). Shoot dry weight and grain yield varied significantly among genotypes and significantly increased with increasing phosphorus (P) levels. Grain harvest index (GHI) and 100-grain weight also differ significantly among genotypes and significantly increased with the increasing P levels. Based on grain yield efficiency index (GYEI), genotypes were classified as efficient and inefficient. The most efficient genotype was CNFP 10104, and inefficient genotypes were CNFP 10103 and CNFP 10120. Number of pods per plant and number of seeds per pod increased significantly with the addition of 200 mg P kg^?1 of soil compared to the low level of P (25 mg P kg^?1). Similarly, nitrogen (N), P, calcium (Ca), magnesium (Mg), sulfur (S), zinc (Zn), copper (Cu), and manganese (Mn) concentrations and uptake in the shoot and grain also significantly varied among genotypes. Uptake of macro- and micronutrients was greater under the greater P rate compared to the low P rate. This may be related to greater shoot or grain yield at 200 mg P kg^?1 soil compared to 25 mg P kg^?1 of soil.     

ROOT GROWTH OF UPLAND RICE GENOTYPES AS INFLUENCED BY NITROGEN FERTILIZATION

The plant root system is an important organ which supplies water and nutrients to growing plants. Information is limited on influence of nitrogen fertilization on upland rice root growth. A greenhouse experiment was conducted to evaluate influence of nitrogen (N) fertilization on growth of root system of 20 upland rice genotypes. The N rate used was 0 mg kg^?1(low) and 300 mg kg^?1(high) of soil. Nitrogen X genotype interactions for root length and root dry weight were highly significant (P < 0.01), indicating that differences among genotypes were not consistent at two N rates. Overall, greater root length, root dry weight and tops-roots ration were obtained at an N fertilization rate of 300 mg kg^?1compared with the 0 mg N kg^?1soil. However, genotypes differ significantly in root length, root dry weight and top-root ratio. Nitrogen fertilization produced fine roots and more root hairs compared with absence of N fertilizer treatment. Based on root dry weight efficiency index (RDWEI) for N use efficiency, 70% genotypes were classified as efficient, 15% were classified as moderately efficient and 15% were classified as inefficient. Root dry weight efficiency index trait can be incorporated in upland rice for improving water and nutrient efficiency in favor of higher yields.     

UPLAND RICE GENOTYPES EVALUATION FOR PHOSPHORUS USE EFFICIENCY

Phosphorus (P) deficiency is one of the most yield limiting factors in crop production in Brazilian Oxisols. A greenhouse experiment was conducted to evaluate 20 upland rice genotypes at low (25 mg P kg^?1) and high (200 mg P kg^?1) P levels applied to a Brazilian Oxisol. Grain yield and yield components were significantly influenced by P level and genotype treatments. There was a significant interaction between P level and genotype treatments in relation to grain yield, indicating genotypes responded differently under two P levels. Based on grain yield efficiency index (GYEI), genotypes were classified into efficient, moderately efficient and inefficient groups. The efficient genotypes in utilizing P were ‘BRA052053’, ‘BRS Primavera’, ‘BRA052015’, ‘BRA052023’, ‘BRA01506’, ‘BRA052045’, ‘BRA032033’, ‘BRA01596’ and ‘BRA052034’. Remaining genotypes were classified as moderately efficient in P use efficiency. None of the genotypes were fall into inefficient group. Grain yield was significantly and positively related with shoot dry weight, panicle number, grain harvest index, 1000-grain weight and had a negative and significant correlation with spikelet sterility. Grain weight was having maximum contribution in total rice plant weight comparing to root and shoot, indicating improvement in harvest index of modern Brazilian upland rice cultivars by breeding.     

DRY BEAN GENOTYPES EVALUATION FOR GROWTH,YIELD COMPONENTS AND PHOSPHORUS USE EFFICIENCY

Dry bean along with rice is a staple food for the population of South America. In this tropical region beans are grown on Oxisols and phosphorus (P) is one of the most yield limiting factors for dry bean production on these soils. A greenhouse experiment was conducted to evaluate P use efficiency in 20 elite dry bean genotypes grown at deficient (25 mg P kg^?1 soil) and sufficient (200 mg P kg^?1) levels of soil P. Grain yields and yield components were significantly increased with P fertilization and, interspecific genotype differences were observed for yield and yield components. The grain yield efficiency index (GYEI) was having highly significant quadratic association with grain yield. Based on GYEI most P use efficient genotypes were CNFP 8000, CNFP 10035, CNFP10104, CNFC 10410, CNFC 9461, CNFC 10467, CNFP 10109 and CNFP 10076 and most inefficient genotypes were CNFC 10438, CNFP 10120, CNFP 10103, and CNFC 10444. Shoot dry weight, number of pods per plant, 100-grain weights and number of seeds per pod was having significant positive association with grain yield. Hence, grain yield of dry bean can be improved with the improvement of these plant traits by adopting appropriate management practices. Soil pH, extractable P and calcium (Ca) saturation were significantly influenced by P treatments. Based on regression equation, optimum pH value in water was 6.6, optimum P in Mehlich 1 extraction solution was 36 mg kg^?1 and optimum Ca saturation value was 37% for dry maximum bean yield.     

Agronomic Evaluation of Dry Bean Genotypes for Potassium Use Efficiency

Dry bean is an important legume worldwide, and potassium (K) deficiency is one of the important constraints for bean production in most of the bean growing regions. A greenhouse experiment was conducted with the objective to evaluate fifteen dry bean genotypes grown on a Brazilian lowland (Inceptisol) United States Soil Taxonomy classification and Gley humic Brazilian Soil Classification system), locally known as “Varzea” soil. The K rate used was 0 mg kg^?1 (low, natural soil level) and 200 mg kg^?1 (high, applied as fertilizer). Straw yield, seed yield, pods per plant, seeds per pod, 100 seed weight, and seed harvest index were significantly increased with the addition of K fertilizer. These traits were also significantly influenced by genotypic treatment. Similarly, root length and root dry weight were also influenced significantly by K and genotype treatments. The K X genotype interactions for most of these traits were also significant, indicating variation in these traits with the variation in K level. Based on seed yield efficiency index (SYEI), genotypes were classified as efficient, moderately efficient, and inefficient in K use efficiency. Maximum grain yield was obtained with 74 mg K kg^?1 extracted by Mehlich 1 extracting solution. Similarly, K saturation required for maximum grain yield was 1.1%.     

Lowland Rice Genotypes Evaluation for Nitrogen Use Efficiency

Rice is important crop for world population, including Brazil. Nitrogen (N) is one of the most yield limiting nutrients in rice production under all agro-ecological conditions. A greenhouse experiment was conducted to evaluate N responses to 12 lowland rice genotypes. Soil used in the experiment was a Gley humic according to Brazilian soil classification system and Inceptisol according to USA soil taxonomy classification. The N rates used were 0 mg kg^?1 (low) and 300 mg kg^?1 (high) of soil. Plant height, straw yield, grain yield, panicle density, 1000 grain weight, and root dry weight were significantly increased with the addition of N fertilization. These growth, yield, and yield components were also significantly influenced by genotype treatment. Grain yield had significant linear or quadratic association with shoot dry weight, panicle number and 1000 grain weight Based on grain efficiency index genotypes were classified as efficient, moderately efficient and inefficient in N use. The N efficient genotypes were ‘BRS Tropical’, ‘BRS Jaçanã’, ‘BRA 02654’, ‘BRA 051077’, ‘BRA 051083’, ‘BRA 051108’, ‘BRA 051130’ and ‘BRA 051250’. Remaining genotypes fall into moderately efficient group. None of the genotypes were grouped as inefficient in N use efficiency.     

Differential Soil Acidity Tolerance of Dry Bean Genotypes

Soil acidity is a major yield-limiting factors for bean production in the tropical regions. Using soil acidity–tolerant genotypes is an important strategy in improving bean yields and reducing cost of production. A greenhouse experiment was conducted with the objective of evaluating 20 dry bean genotypes for their tolerance to soil acidity constraints. An Inceptisol soil was amended with dolomitic lime (2 g dolomitic lime kg^–1 soil) to achieve low acidity (pH = 5.9) and without lime (zero lime kg^–1 soil,) to achieve high acidity (pH = 4.8) levels to evaluate bean genotypes. At both acidity levels, genotypes differed significantly in shoot dry weight and grain yield. Shoot dry weight and grain yield were significantly decreased at the high acidity level compared to the low acidity level. Grain yield was more sensitive to soil acidity than shoot dry weight. Hence, grain yield was used in determination of tolerance index (GTI) to differentiate the range of soil acidity tolerance among bean genotypes. Based on a GTI value, 55% of the genotypes were classified as tolerant, 40% classified as moderately tolerant, and the remaining were grouped as susceptible to soil acidity. The genotype CNFC 10410 was most tolerant and genotype CNFP 10120 was most susceptible to soil acidity. Number of pods and grain harvest index were significantly and positively associated with grain yield. The improvement in grain yield in low acidity may be related to reduction of toxic levels of soil aluminum (Al³⁺) and hydrogen (H⁺) ions by lime addition. At harvest, soil extractable phosphorus (P) and potassium (K) increased with the reduction of soil acidity, and this might have contributed to the better nutrition of beans and lead to higher growth.     

GENETICALLY MODIFIED AND CONVENTIONAL DRY BEAN GENOTYPE RESPONSES TO SOIL FERTILITY

Dry bean is important pulse for the diet of South American population and results related to comparison of genetically modified and conventional dry bean genotypes to soil fertility are limited. A greenhouse experiment was conducted to compare genetically modified and conventional dry bean genotypes to soil fertility. Genotypes evaluated were Olathe Pinto, Olathe 5.1 (genetically modified), BRS Pontal, BRS Pontal 5.1 (genetically modified), Pérola and Pérola 5.1 (genetically modified). Fertility levels were 1 g fertilizer (5-30-15) kg^?1 soil (low fertility level) and 2 g fertilizer (5-30-15) per kg soil (high fertility level). These fertility levels were designated as low and high, respectively. Grain yield, number of pods per plants, and seed per pod were significantly increased with the increase in soil fertility. Shoot dry weight, seed per pod, and 100 seed weight were also significantly influenced by genotype treatment. Fertility X genotypes interaction was significant for maximum root length and root dry weight, indicating genotypes responded differently at two fertility levels in relations to these two traits. Shoot dry weight, number of pods per plant, and grain harvest index had significant association with grain yield, indicating that increase in these three traits grain yield can be increased. Grain yield efficiency index (GYEI) was having significant linear association with grain yield. Hence, on the basis of GYEI, genotypes were classified as efficient (E), moderately efficient (ME), and inefficient in nutrient use. Three conventional genotypes (Olathe Pinto, BRS Pontal and Pérola) and one genetically modified genotype (Olathe Pinto 5.1) were classified as moderately efficient and two genetically modified genotypes (Pérola 5.1 and BRS Pontal 5.1) were classified as efficient. None of the genotypes fall into the inefficient group.     

Genotypic Differences in Dry Bean Yield and Yield Components as Influenced by Nitrogen Fertilization and Rhizobia

Dry bean (Phaseolus vulgaris L.) is an important legume worldwide and nitrogen (N) is most yield limiting nutrients. A field experiment was conducted for two consecutive years to evaluate response of 15 dry bean genotypes to nitrogen and rhizobial inoculation. The N and rhizobia treatments were (i) control (0 kg N ha^?1), (ii) seed inoculation with rhizobia strains, (iii) seed inoculation with rhizobia strains + 50 kg N ha^?1, and (iv) 120 kg N ha^?1. Straw yield, grain yield, and yield components were significantly influenced by N and rhizobial treatments. Grain yield, straw yield, number of pods m^?2, and grain harvest index were significantly influenced by year, nitrogen + rhizobium, and genotype treatments. Year × Nitrogen + rhizobium × genotype interactions were also significant for these traits. Hence, these traits varied among genotypes with the variation in year and nitrogen + rhizobium treatments. Inoculation with rhizobium alone did not produce maximum yield and fertilizer N is required in combination with inoculation. Based on grain yield efficiency index, genotypes were classified as efficient, moderately efficient, and inefficient in nitrogen use efficiency (NUE). NUE defined as grain produced per unit N applied decreased with increasing N rate. Overall, NUE was 23.17 kg grain yield kg^?1 N applied at 50 kg N ha^?1 and 13.33 kg grain per kg N applied at 120 kg N ha^?1.     

Phosphorus Nutrition of Upland Rice,Dry Bean,Soybean, and Corn Grown on an Oxisol

Rice, dry bean, corn, and soybean are important food crops. Phosphorus (P) deficiency is one of the most yield-limiting factors for these crops grown on highly weathered Brazilian Oxisols. Four greenhouse experiments were conducted to determine P requirements of these four crops. The P levels used were 0, 50, 100, 200, and 400 mg kg^?1. Growth, yield, and yield components evaluated of four crop species were significantly increased with the application of P fertilization. Most of the responses were quadratic in fashion when the P was applied in the range of 0 to 400 mg kg^?1. Maximum grain yield of upland rice was obtained with the application of 238 mg P kg^?1 of soil, maximum dry bean grain yield was obtained with the application of 227 mg P kg^?1 of soil, and maximum grain yield of soybean was obtained with the application of 224 mg P kg^?1 of soil. Maximum shoot growth of corn was obtained with the addition of 323 mg P kg^?1 of soil. Most of the growth and yield components had significant positive association with grain yield or shoot dry weight. Phosphorus concentration and uptake were greater in the grain compared to straw in upland rice and dry bean plants. Overall, P-use efficiencies decreased with increasing P rates.     

Phosphorus Use Efficiency in Upland Rice Genotypes Under Field Conditions

Phosphorus (P) deficiency is one of the most yield limiting factors for crop production in South American soils. Upland rice (Oryza sativa L.) is an important crop in South American cropping systems, including Brazil. A field experiment was conducted with the objective to evaluate 20 upland rice genotypes for phosphorus (P) use efficiency. The P rate used was low (0 kg P ha^?1) and high [87 kg P ha^?1 or 200 kg phosphorus pentoxide (P₂O₅) ha^?1]. Plant height, shoot dry weight, grain yield, panicle number, 1000 grain weight, spikelet sterility, and grain harvest index were significantly influenced by P and genotype treatments. The P X genotype interaction was significant for grain yield, indicating that genotypes responded differently under two P rates. Overall, grain yield increased by 12% with the addition of P fertilization. Based on grain yield efficiency index, genotypes were classified into efficient, moderately efficient, and inefficient group. The genotypes that were classified as efficient in P use were BRA032048, BRA042094, BRA02601, BRA032051, BRA032033, BRA052015, BRA042156, BRA01600, BRA01506, BRA052023 and BRA042160. The inefficient genotypes in P us efficiency were BRS Primavera, BRA052045, BRA01596, and BRS Sertaneja. Grain harvest index had a significant positive association with grain yield and spikelet sterility had a significant negative association with grain yield, as expected. Average, P-use efficiency of five genotypes was about 17 kg kg^?1 (kg grain yield per kg P applied).     

Screening Upland Rice Genotypes for Manganese‐use Efficiency

Manganese (Mn) deficiency in upland rice grown after common bean or soybean, which received adequate rate of liming on highly weathered Oxisols, is observed. A greenhouse experiment was conducted to evaluate Mn‐use efficiency of 10 promising upland rice genotypes. The genotypes were grown on an Oxisol at 0 mg Mn kg^?1 (natural soil Mn level) and 20 mg Mn kg^?1 of soil applied as manganese sulfate. Grain yield, panicle number, and grain harvest index (GHI) were significantly (P < 0.01) influenced by genotype. However, shoot dry weight was significantly affected by Mn as well as genotype treatments. Manganese uptake in the shoot as well as in the grain was also affected by genotype treatment. On the basis of Mn‐use efficiency (mg grain weight/mg Mn accumulated in shoot and grain), genotypes were classified as efficient and responsive (ER), efficient and nonresponsive (ENR), nonefficient and responsive (NER), and nonefficient and nonresponsive (NENR). Genotypes Carisma, CNA8540, and IR42 were classified as ER, and genotypes CNA8557 and Maravilha were classified as ENR. Genotype Caipo was in the group NER, and in the NENR group were genotypes Bonança, Canastra, Caraja, and Guarani. From a practical point of view, genotypes that produce high grain yield at a low level of Mn and respond well to Mn additions are the most desirable because they are able to express their high yield potential in a wide range of Mn availability.     

Yield and Yield Components and Phosphorus Use Efficiency of Lowland Rice Genotypes

Phosphorus deficiency is main constraints for lowland rice production in various rice producing regions of the world. A greenhouse experiment was conducted using lowland (Inceptisol) soil with the objective to determine response of seven lowland rice (Oryza sativa L.) genotypes to phosphorus fertilization and to evaluate their phosphorus (P) use efficiency. Phosphorus treatments included control (0 mg P kg^?1) and 200 mg P kg^?1 of soil. Plant height and shoot dry weight were significantly (P < 0.001) influenced by P treatments. Phosphorus X genotypes interaction was significant for shoot dry weight, indicating different response of genotypes under two P levels. At low P level, none of the genotypes produced grain yield, indicating original P level in the soil was too low for lowland rice yield. However, genotypes differed significantly in grain yield at high P level. Panicle number, panicle length, and thousand grains weight had a significant quadratic association with grain yield. However, spikelet sterility had a significant linear negative association with grain yield. The P use efficiency expressed as agronomic efficiency (AE), physiological efficiency (PE), agro-physiological efficiency (AP), apparent recovery efficiency (ARE), and utilization (UE) were significantly different among genotypes. These efficiencies were having significantly positive association with grain yield, with exception to ARE, indicating improving grain yield with improved P use efficiencies in rice.     

Soil Phosphorous Influence on Growth and Nutrition of Tropical Legume Cover Crops in Acidic Soil

In tropical regions, use of cover crops in crop production is an important strategy in maintaining sustainability of cropping systems. Phosphorus (P) deficiency in tropical soils is one of the most yield-limiting factors for successful production of cover crops. A greenhouse experiment was conducted to evaluate influence of P on growth and nutrient uptake in 14 tropical cover crops. The soil used in the experiment was an Oxisol, and P levels used were low (0 mg P kg^?1), medium (100 mg P kg^?1) and high (200 mg P kg^?1). There was a significant influence of P and cover crop treatments on plant growth parameters. Phosphorus X cover crops interaction for shoot dry weight, root dry weight and root length was significant, indicating different responses of cover crops to variable P levels. Based on shoot dry weight efficiency index (SDEI), legume species were classified into efficient, moderately efficient or inefficient groups. Overall, white jack bean, gray mucuna bean, mucuna bean ana and black mucuna bean were most P efficient. Remaining species were inefficient in P utilization. Macro- and micronutrient concentrations (content per unit dry weight of tops) as well as uptakes (concentration x dry weight of tops) were significantly (P < 0.01) influenced by P as well as crop species treatments, except magnesium (Mg) and zinc (Zn) concentrations. The P x crop species interactions were significant for concentration and uptake of all the macro and micronutrients analyzed in the plant tissues, indicating concentrations and uptake of some nutrients increased while others decreased with increasing P levels. Hence, there was an antagonistic as well as synergetic effect of P on uptake of nutrients. However, uptake of all the macro and micronutrients increased with increasing P levels, indicating increase in dry weight of crop species with increasing P levels. Overall, nutrient concentration and uptake in the top of crop species were in the order of nitrogen (N) > potassium (K) > calcium (Ca) > Mg > sulfur (S) > P for macronutrients and iron (Fe) > manganese (Mn) > zinc (Zn) > copper (Cu) for micronutrients. Interspecific differences in shoot and root growth and nutrient uptake were observed at varying soil P levels     

NITROGEN USE EFFICIENCY IN DRY BEAN GENOTYPES

Dry bean is an important legume crop for Latin American people and nitrogen is one of the most yields limiting nutrients for bean crop. A greenhouse experiment was conducted to evaluate nitrogen (N) use efficiency of 20 dry bean genotypes. Genotypes were grown on an Oxisol and two N levels used were without N application (low level) and an application of 400 mg N kg^?1 (high level). Shoot dry weight, grain yield and yield components, N concentration and uptake in shoot and grain were significantly affected by N and genotype treatments. Grain yield had a highly significant (P < 0.01) association with shoot dry weight, pod number, grains per pod and 100 grain weight. Among the 20 genotypes tested, Perola, CNFR 7847, CNFR 7865, CNFP 7777 and CNFM 6911 were found to produce reasonably good yield at low N rate as well as responded well to applied N. Whereas, some genotypes like BRS Radiante, CNFP 7624, CNFM 7875, CNFM 7886, CNFC 7813, CNFC 7827, CNFP 7677 and CNFP 7775 produced very good yields at higher N rate but very low yields at lower N rate. Hence, these genotypes are good for farmers using higher technology. Nitrogen concentration and uptake were higher in dry bean grains compared with shoot and 63% of N accumulated at zero N rate and 75% N accumulated at 400 mg N rate were translocated to grain across 20 genotypes. Nitrogen uptake efficiencies were having highly significant (P < 0.01) quadratic relationship with grain yield. This indicates that improving N uptake in dry bean plants can increase grain yield.