Oil hydrocarbons are widespread pollutants in soil which pose serious threats to ecological environment. Thus, this study carried out the bioremediation of oil-contaminated soil by using the efficient petroleum-degrading bacteria and soil conditioner, to investigate the changes of physicochemical properties of contaminated soil during bioremediation, reveal the relationship among the exogenous degradation strains and indigenous microbe, and finally illuminate the effects of soil conditioner and microbe on the bioremediation of oil-contaminated soil.
Materials and methodsA PAH-degrading strain named Stenotrophomonas maltophilia was used in this study, which was isolated from an e-waste dismantling area. The soil conditioner in this present study was developed previously by using agricultural wastes, which was in a powdered form and rich in N, P, and K. The simulated experiments were conducted under the control environmental conditions of greenhouse, to study the effects of inoculation and soil conditioner on bioremediation of oil-contaminated soil. Then, the physicochemical properties of soil and the degradation rates of oil were measured at different set times to evaluate the bioremediation effect.
Results and discussionAdding 1% soil conditioner could significantly improve the soil conditions and offer microorganism enough N, P, and K, which would promote microbial growth and played a key role on bioremediation of oil-contaminated soil. Although in polluted soil, the microorganism could maintain metabolic activity and use the petroleum as carbon source. The soil indigenous microbe was more easily to adapt to the contaminated surrounding. However, when both of them co-existed in soil, they would restrain each other, and go against the oil decomposition. Thus, making two types of microorganisms work to achieve synergy was the key to gain much better remediation effect. Because the indigenous microbe was good at decomposing low molecular compounds and saturated hydrocarbons, while the oil-degrading strains can effectively decompose high molecular weight aromatics.
ConclusionsThe soil nutrient and microorganism, including the exogenous oil-degrading strains and the soil indigenous microbe, had an important effect on degradation of petroleum. The addition of soil conditioner, presence of indigenous microbe, and inoculation of oil-degrading strains all were conducive to bioremediation of oil-contaminated site, but the key was to control the proportion and relationship of the three.
相似文献Information on root-zone soil water content (SWC) is essential for vegetation restoration, irrigation scheduling, and hydrological modeling. However, measurements of SWC within a variety of land uses may be time-consuming and labor-costing. This study tested whether SWC at a depth of a land use can be used to predict profile SWC of other land uses in terms of temporal stability analysis at a karst depression area in southwest China.
Materials and methodsA total of 30 datasets of root-zone SWC from 0.1- to 0.5-m depths were collected by time domain reflectometry probes for three typical land uses from March 12 to November 8, 2015.
Results and discussionResults showed that the profile mean SWC and its associated standard deviation (SDP) and coefficient of variation (CVP) differed significantly (P?<?0.05) among the grassland, farmland, and forestland. The profile SWC was more temporally stable according to the apparently lower CVT in comparison with CVP. The similarities of the vertical patterns of SWC were strong for the same land uses, while were relatively weak between the different land uses. The SWC measurements of the most temporally stable depth can be used to accurately predict profile SWC for both the same land use and other land uses.
ConclusionsThis study further expands the application of the temporal stability analysis and can aid water resource management in areas with diverse land uses.
相似文献Large spoil heaps formed during construction projects have caused serious soil erosion and threatened ecological security. The recent researches on soil erosion of spoil heaps are based on one or several soil types, which can only represent the soil texture category within the limited area, but cannot be used in other larger scale areas. Soil texture and gravel are the main factors affecting infiltration and erosion processes of spoil heaps.
Materials and methodsThe runoff plot dimensions were 5.0 m?×?1.0 m?×?0.5 m (length × width × depth). A series of rainfall experiments with a constant rainfall intensity of 1.0 mm min?1 and a slope gradient of 25° were conducted to investigate the effects of soil texture (sandy, loam, and clay) and gravel mass content (GC, 0%, 10%, 20%, and 30%) on the infiltration and erosion processes. The gravels are divided into 3 classes according to particle size 2–14 mm (small), 14–25 mm (medium), 25–50 mm (large), and the mass ratios were 30%, 50%, and 20%. The duration of each rainfall event was 45 min after runoff out of the plot.
Results and discussionResults showed that there was a critical GC (10%) improving or controlling infiltration and soil loss. Infiltration rate of sandy spoil heap (SSH) decreased within 45 min, but it decreased first and then stabilized for loam spoil heap (LSH) and clay spoil heap (CSH). Soil loss rate (SLR) of SSH stabilized first and then increased, while it decreased and then stabilized for LSH and CSH. SLR at early stage (0–18 min) was 0.08–0.23 times than it was at later stage (18–45 min) for SSH, but it was 2.06–5.06 times and 1.46–1.95 times for LSH and CSH, respectively. The soil texture had a more significant effect on SLR (P?< 0.05) than GC did. The effects of gravel on SLRs were dependent on soil texture.
ConclusionsThe greater the GC was, the lower the SLR was for the spoil heaps. Special attention should be paid to the later stage during rainfall events for SSHs and the early stage for LSHs and CSHs when considering erosion protection measures.
相似文献The purpose of this paper is to study the responses of soil biological parameters as indicator of ecological status on PAH-contaminated soil.
Materials and methodsStudies are conducted on the soils and natural grassy vegetation of monitoring plots subjected to Novocherkassk power station (NPS) emissions. Monitoring plots were established at different distances from the NPS (1.0–20.0 km).
Results and discussionThe level of polycyclic aromatic hydrocarbons (PAHs) around NPS is the highest at the monitoring plot located at distance 1.6 km to the northwest through the prevailing wind direction. Gradually, decrease of PAHs was observed while increasing the distance from the NPS through the prevailing wind direction. Calculation of correlations between PAH level and biological activity parameters of soils showed lack of dependence with total and every PAH content in all 12 studied monitoring plots. The most significant correlations were found between PAH content and enzyme activity in the monitoring plots situated through the prevailing wind direction from NPS.
ConclusionsThe main pollution source in the studied area is NPS. It was found that contamination of soil by PAHs has a direct dependence on the activity of all biological communities in chernozems, as well as the activities of dehydrogenase and the phytotoxicity of soils. Inverse correlations have been revealed between the PAH contamination and abundance of soil bacteria.
相似文献Soil consists of various sizes of aggregates, and different soil aggregates vary in their abilities to adsorb or transport metals. This study aimed to investigate the distribution behaviors of Cu from different fungicides in soil aggregates after a 15-month incubation.
Materials and methodsBordeaux mixture (CuSO4/Ca(OH)2?=?1/1 by weight, BR), copper nitrate (Cu(NO3)2·4H2O, CN), and copper oxychloride (3Cu(OH)2·CuCl2, CO) were applied to a representative Chinese Mollisol to reach the Cu content 200 mg kg?1. Five soil aggregate fractions, i.e., >?2000 μm, 2000–1000 μm, 1000–500 μm, 500–250 μm, and <?250 μm, were obtained by the wet sieving method. The modified Bureau Communautaire de Références (BCR) sequential extraction was applied to assess the Cu distribution among the main soil fractions.
Results and discussionThe highest Cu mass loading was found for the >?2000-μm soil aggregate. The input Cu was mainly in stable fractions, and the highest proportion was found for the residual fraction. The bioavailability and mobility of Cu from different fungicides in soils varied from each other, and they presented an order of CO > CN > BR. High bioavailability and transferring coefficients were found in the <?250-μm and >?2000-μm soil aggregates.
ConclusionsThis study indicated that the input Cu from fungicides mainly distributed in the >?2000-μm soil aggregates. Moreover, the CO-derived Cu presented a higher availability than the BR- and CN-derived Cu in the soil.
相似文献Many studies have shown the simulated effects of nitrogen (N) deposition on soil microbial community composition by adding N directly to the forest floor but have ignored the N retention process by the canopy. This study was conducted to compare the responses of soil microbial biomass and community composition between soil application of N (SAN) and foliage application of N (FAN).
Materials and methodsA pot experiment was designed with (1) two N application methods (SAN and FAN), (2) three N application levels (5.6, 15.6 and 20.6 g N m?2 year?1), and (3) two tree species (Schima superba Gardn. et Champ. and Pinus massoniana Lamb.) following a nested factorial design. Soil microbial biomass and community composition were determined using phospholipid fatty acids (PLFAs) techniques after 1 and 1.5 years of treatments.
Results and discussionNitrogen addition increased (P?<?0.05) soil NH4+-N content and soil NO3?-N content and decreased (P?<?0.05) soil pH and soil microbial (bacterial, fungal, and actinomycete) biomass for both N application methods. Compared with the SAN treatment, the FAN treatment had higher (P?<?0.05) pH and lower (P?<?0.05) contents of soil NH4+-N and soil NO3?-N. Soil microbial biomass and community composition were significantly different between the different N addition levels under the SAN treatment, but they showed no significant difference (P?<?0.05) between the different N addition levels under the FAN treatment. The soil microbial biomass in the S. superba soil was higher (P?<?0.05) than that in the P. massoniana soil for the FAN treatment, with the opposite trend observed under the SAN treatment. Moreover, redundancy analysis showed that soil microorganisms were significantly correlated with soil pH, soil water content, NH4+-N, and NO3?-N.
ConclusionsThe results showed that N addition affected soil properties, microbial biomass, and the composition of microbial communities; however, the FAN treatment had less influence on soil properties and soil microorganisms than did the SAN treatment over short time scales, and the extent of this effect was different between coniferous and broadleaf trees.
相似文献A field experiment was conducted to assess the role of nitrogen (N) and water addition in shaping soil fungal communities and co-occurrence networks in temperate grassland, northern China.
Materials and methodsWe measured soil fungal and plant community compositions, and also soil properties including available N, phosphorus, potassium concentrations, soil pH, and soil moisture. Soil fungal co-occurrence networks were constructed using a random matrix theory–based network inference approach.
Results and discussionPlant species richness was decreased by N addition but increased by water addition, whereas fungal richness was decreased by N addition. The fungal community composition was significantly changed by both N addition and water addition. Soil fungal α diversity and β diversity were explained by a combination of variations in plant species richness and plant functional composition, and also by changes in soil pH via the soil acidification pathway induced by N and water addition. The fungal co-occurrence networks were more complex and clustered under water addition than that in ambient precipitation.
ConclusionsOur results suggested that plant functional composition, plant species richness, and soil acidification should be incorporated into ecosystem models for predicting soil fungal communities under future climate changes in terrestrial ecosystems.
相似文献Infiltration modeling is an important tool to describe the process of water infiltration in the soil. However, direct measurements of the parameters of infiltration models are usually time-consuming and laborious. The present study proposed an effective method to estimate parameters of the Kostiakov-Lewis model (a classical infiltration model) from soil physical properties (SPPs).
Materials and methodsParameters k, α, and f0 of the Kostiakov-Lewis infiltration models were measured in 240 double-ring field experiments in Shanxi Province, China. SPPs at the corresponding experimental points were measured at the topsoil layer (TL, 0–20 cm) and the top-subsoil layer (TSL, 0–20 and 20–40 cm). The Kennard-Stone (KS) sampling method and principal component analysis (PCA) were used for dividing training samples and extracting principal components (PCs) of SPPs, respectively. Partial least squares (PLS), back-propagation neural networks (BPNNs), and a support vector machine (SVM) were used to establish models for estimating k, α, and f0 with the SPPs of TL and TSL as the input variables (IV).
Results and discussionThe differences in soil density (BD), texture, and moisture content (θv) were found in topsoil and subsoil, but loading distributions of SPPs on PCs present different degrees of correlation. Moreover, SVM produced the most accurate estimation among these three methods for using the SPP of TL and TSL as inputs. The highest accuracy for k estimations was obtained by SVM using the SPP of TL as IV; R and RMSE in the model test process were 0.78 and 0.3 cm min?1, respectively. However, using SPP of TSL as IV obtained the highest accuracy for both α and f0 estimations with the SVM method (R values were 0.71 and 0.82, respectively, and RMSE values were 0.03 and 0.018 cm min?1) in the model testing.
ConclusionsThe SVM method with SPPs as inputs is an effective and practical method for estimating the parameters of the Kostiakov-Lewis infiltration model.
相似文献Vanadium (V) contamination in soil can cause diverse damage to soil ecosystem and has attracted research interests in exploring soil V stabilization methods, but only a few materials were proposed and studied. Here, a pot experiment was firstly conducted to estimate the efficiency of nano-hydroxyapatite (n-HAP) in stabilizing V in soil. To verify the impact of n-HAP on soil V bioavailability and phytotoxicity, cabbages (Brassica chinensis L.) were grown in V-spiked soils after n-HAP amendment.
Materials and methodsSoils were sampled from a farmland in China, and the n-HAP was prepared in the laboratory. In each pot of soil spiked with 0, 75, 150, 300, and 600 mg/kg V, 2% n-HAP was amended for 30 days, while soils without n-HAP amendment were set as controls. The stabilization effect of n-HAP on V in soil was estimated by the water-extractable and bioavailable V concentrations in soils. Cabbages were grown in pots subsequently. The V(V/IV) concentrations in cabbage leaves and roots, the organic bound V concentrations in cabbage roots, and the chlorophyll concentrations in leaves were determined. Bioconcentration factor and translocation factor were calculated. The composition of organic bound V in leaf was characterized by fluorescence excitation–emission matrix.
Results and discussionIn soils spiked with 150 mg/kg V, n-HAP amendment yielded the highest stabilization rates of 51.0% and 42.4% for water-extractable and bioavailable V, respectively. In 75, 150, and 300 mg/kg V-spiked soil, the plant weight, plant height, and root length of cabbage after 60-day growing decreased 54.6%/89.6%, 30.9%/45.5%, and 41.5%/51.4% in groups with/without n-HAP, respectively. Cabbage leaf chlorophyll concentrations descend firstly then ascend with rising soil V concentration. Leaf V speciation analysis revealed that less leaf V was reduced to V(IV) in groups amended with n-HAP than groups without n-HAP amendment. In 150 and 300 mg/kg V-spiked soil, n-HAP effectively reduced the V content and the V bioconcentration factor of cabbage root. Tyrosine-like and humic acid-like analogues composed the principal part of V complex.
ConclusionsIn general, n-HAP amendments are potential to decrease the mobility of V in soils, as well as inhibit the bioavailability and phytotoxicity of V to cabbage. In V-spiked soils, n-HAP amendment can alleviate the toxicity of V to the cabbage. Overall, 2% n-HAP is efficient for the amendment of slight V-polluted (150–300 mg/kg) soils to alleviate the soil V stress to cabbage.
相似文献Soil health has been a major focus of research by international scholars because it is an important factor that supports human survival and development. However, few scholars have performed bibliometric analyses of research on soil health. This study investigated the current research status and development characteristics of soil health.
Materials and methodsWe performed a bibliometric analysis of documents on soil health from 1999 to 2018 in the Web of Science Core Collection. The bibliometric analysis tools, CiteSpace and HistCite software, were applied.
Results and discussionA total of 1629 documents on soil health in the Web of Science Core Collection were identified. From the research status of soil health, the number of publications will continue to increase. Most of the research institutions examining soil health are located in the USA, and there is little cooperation and communication among countries, neither do research institutions in this field. Each country has its own research labs while lab cooperation within individual countries is relatively close. Most documents on the research of soil health have been published in the journal Applied Soil Ecology. In addition, research directions are expanding from the basic study of soil production, soil health indicators, and soil pollution to the comprehensive study of soil ecosystem.
ConclusionsBibliometric analysis tools, CiteSpace and HistCite software, provide a comprehensive approach for making predictions regarding trends in the field. This method was used in this study to identify the hotspots, frontiers, and future development of soil health to provide guidance for future research.
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