MORPHOLOGICAL RESPONSE OF FORAGE CHICORY FINE ROOTS TO MANIPULATED SOIL PHOSPHORUS LEVELS

In Appalachian pastures, chicory (Cichorium intybus L.) offers the potential for increased phosphorus (P) uptake. To determine if hydroponically grown chicory root response patterns also occur with soil grown plants, an experiment with three cultivars and four soil P levels was conducted. Roots were washed free of soil and then measured, then plant tissues were analyzed for P content. With decreasing soil P concentrations, “Grasslands Puna” decreased the diameter and increased the length of the thinnest roots. The cultivar “Forage Feast” responded to low P with the opposite pattern. Two populations of root diameter classes (meso-diameter classes - mDC) are shown to be involved in these responses. With Grasslands Puna, the larger diameter mDC is apparently suppressed at low P levels in favor of the smaller diameter mDC. The reverse is true with Forage Feast. Thus, chicory cultivars have differential fine root responses to soil P levels.     

Response of Forage Chicory Seedlings to Available Soil Phosphorus in Two Soils in a Controlled Environment

Poor phosphorus (P) fertility is a problem limiting productivity on unimproved Appalachian soils and has been implicated in poor palatability of forage chicory (Cichorium intybus L.). Three cultivars were grown in a greenhouse at varying soil P levels in Gilpin and Hagerstown silt loam soils and harvested 48 and 77 days after planting. In Gilpin soil, available soil P (ASP) was positively associated with dry shoot mass, leaf mass, leaf area, and leaves per plant and negatively associated with specific leaf area. In Hagerstown soil, ASP was positively associated only with only dry shoot and leaf mass. At equivalent ASP levels, dry shoot and leaf mass, leaf area, and shoot concentrations of P, potassium, calcium, and magnesium were always greater in Hagerstown than Gilpin soil, whereas specific leaf area was less. Equalization of ASP concentration and environmental conditions did not eliminate growth-limiting differences between Gilpin and Hagerstown soils for chicory.     

Carbon distribution and variations in nitrogen-uptake between catch crop species in pot experiments

Chicory (Cichorium intybus L.) and perennial ryegrass (Lolium perenne L.) are seen as suitable catch crops species in Sweden. Pot experiments were conducted to study C distribution and variations in nitrogen uptake between several varieties of chicory and perennial ryegrass for comparison.A soil amended with Ca(¹⁵NO₃) (109 and 145 mg N kg⁻¹ soil) and glucose (2.5 g C kg⁻¹ soil) was incubated for 10 days to promote the immobilization of added ¹⁵N; therefore, N was supplied to plants through the remineralization of the immobilized ¹⁵N. In experiment 1 four varieties of chicory and one variety of perennial ryegrass were grown for 60 days in greenhouse conditions. In experiment 2, only two varieties of chicory and one ryegrass were grown in soil with high-N rate of fertilization. In the later experiment, pots were moved from greenhouse to a growth chamber with ¹⁴CO₂ atmosphere for a pulse labelling of the plants 7–10 days before harvest.At both levels of N supply, dry weights of taproots were higher in the chicory cultivars Cassel and Fredonia than in cultivars Puna and Salsa. The opposite was found for dry weights of small roots. There were significant differences in N uptake between chicory varieties. Cassel and Fredonia together with the ryegrass were significantly more effective in securing nitrate than the other two varieties. Significantly higher amounts of labelled-N were found in taproots of Cassel than in Puna. The opposite trend was found for small roots. Similar results were measured for amounts of radioactivity (kBq pot⁻¹) of newly fixed C transferred to roots. Amounts of labelled-N measured in soil residues for both crop species were significantly higher at the low level of N supply than at the high level of N. There was no significant increase in plant uptake of soil-N (native-N) either between chicory varieties or between chicory and ryegrass, when the high level of N was supplied.The importance of these results is discussed in relation to the suitability of chicory species as catch crop and as plant material for breeding.     

Effect of nitrogen rate on plant nitrogen loss in winter wheat varieties 1

Gaseous nitrogen (N) loss from winter wheat (Triticum aestivum L.) plants has been identified, but has not been simultaneously evaluated for several genotypes grown under different N fertility. Two field experiments were initiated in 1993 and 1994 at the Agronomy Research Station in Stillwater and Perkins to estimate plant N loss from several cultivars as a function of N applied and to characterize nitrogen use efficiency (NUE). A total of five cultivars were evaluated at preplant N rates ranging from 30 to 180 kg·ha^‐1. Nitrogen loss was estimated as the difference between total forage N accumulated at anthesis and the total (grain + straw) N at harvest. Forage, grain, straw yield, N uptake, and N loss increased with increasing N applied at both Stillwater and Perkins. Significant differences were observed among varieties for yield, N uptake, N loss, and components of NUE in forage, grain, straw, and grain + straw. Estimates of N loss over this two‐year period ranged from 4.0 to 27.9 kg·ha^‐1 (7.7 to 59.4% of total forage N at anthesis). Most N losses occurred between anthesis and 14 days post‐anthesis. Avoiding excess N application would reduce N loss and increase NUE in winter wheat varieties. Varieties with high harvest index (grain yield/total biomass) and low forage yield had low plant N loss. Estimates of plant loss suggest N balance studies should consider this variable before assuming that unaccounted N was lost to leaching and denitrification.     

Broad tuning of the human bitter taste receptor hTAS2R46 to various sesquiterpene lactones, clerodane and labdane diterpenoids, strychnine, and denatonium

Sesquiterpene lactones are a major class of natural bitter compounds occurring in vegetables and culinary herbs as well as in aromatic and medicinal plants, where they often represent the main gustatory and pharmacologically active component. Investigations on sesquiterpene lactones have mainly focused on their bioactive potential rather than on their sensory properties. In the present study, we report about the stimulation of heterologously expressed human bitter taste receptors, hTAS2Rs, by the bitter sesquiterpene lactone herbolide D. A specific response to herbolide D was observed i.a. for hTAS2R46, a so far orphan bitter taste receptor without any known ligand. By further investigation of its agonist pattern, we characterized hTAS2R46 as a bitter receptor broadly tuned to sesquiterpene lactones and to clerodane and labdane diterpenoids as well as to the unrelated bitter substances strychnine and denatonium.     

YIELD AND MINERAL CONCENTRATIONS OF SOUTHEASTERN UNITED STATES OAT CULTIVARS USED FOR FORAGE

A shift in oat (Avena sativa L.) production from grain to forage (hay and grazing) is occurring in the southeastern USA. However, most available cultivars were developed for improved grain production, rather than forage yield. We field tested several standard and new oat releases over 2 years, using repeated clippings to determine forage yield, nutrient uptake, and the potential to match plant nutrients with cattle mineral dietary needs. There were no differences in total annual forage yield among the tested cultivars within years but there was a difference between years. Forage tissue phosphorus (P), magnesium (Mg), and calcium (Ca) were sufficient, potassium (K), sulfur (S), and manganese (Mn) were excessive, and iron (Fe), copper (Cu), and zinc (Zn) were occasionally or frequently deficient to meet daily cattle dietary mineral needs. Sulfur, Cu, Fe, Zn, and Mn may be the most challenging to regulate in U.S. Coastal Plain soils at concentrations that satisfy both, oat and cattle nutritional requirements.     

Response of tall fescue to fertilizer placement at different levels of phosphorus,potassium, and soil pH 1

Fertilizer application, particularly nitrogen (N), is important in cool‐season grass forage production. Subsurface (knife) placement of N often has resulted in higher forage yield and N uptake of tall fescue (Festuca arundinacea Schreb.) compared to surface‐broadcast fertilization, but further studies were needed to indicate whether soil pH, phosphorus (P), or potassium (K) modifies the response. Experiment I tested responses of forage yield and N and P concentration to N, P, and K amount and placement. Two types of fertilizer placement ‐ broadcast and knife ‐ were used with 13, 112, or 168 kg N; 0 or 19 kg P; and 0 or 37 kg K/ha in a factorial arrangement. Yields increased by 53% as N fertilization went from 13 to 112 kg/ha and by 69% as N increased from 13 to 168 kg N/ha. Forage yield was increased 26% from knife compared to broadcast fertilizer placement. P application increased forage production by 13%, but K application had no effect on yield. Forage N concentrations increased by 25% as N fertilization went from 13 to 112 kg/ha and by 38% as N increased from 13 to 168 kg N/ha. Effects of added P and fertilizer placement on N concentration often resulted in interactions among factors. Forage P generally was increased by added P, with some effects of interactions among N rate, P rate, and placement. In Experiment II, fescue responses to N placement were tested where different soil characteristics had been established by previous lime and fertility treatments. Forage yield, N concentration, and N uptake were highest where 9.36 Mg/ha of lime were applied as compared to the control. Previous fertility treatments had no significant (P<0.05) effect. When N was knifed, forage yield was related positively to available soil P but not to pH or K. Yield and forage N concentration and uptake were increased by 20, 11, and 33%, respectively, as a result of knife versus broadcast N application.     

Forage Nutritional Quality Evaluation of Bahiagrass Selections during Autumn in Florida

Bahiagrass (Paspalum notatum Flugge) is the major pasture forage in the southern Gulf Coast, USA. A bahiagrass selection breeding program has been ongoing since 1960 at the Coastal Plain Experiment Station at Tifton, Georgia, USA, to increase forage yield in Pensacola (P. notatum var. sanese) bahiagrass. However, the impact of selecting for forage yield on forage nutritional quality is unknown. Forage quality was evaluated on four Pensacola derived selection cycles (C) of bahiagrass [C0 (Pensacola), C4, C9 (Tifton 9), and C23]. A total of 175 plants per cycle were grown. Forage from individual 1-year-old plants was harvested by hand on 3 October and again on 15 November 2000. The samples were dried, ground, and analyzed using internally calibrated near-infrared reflectance spectroscopy (NIRS) for dry matter (DM), in vitro–digestible organic matter (IVDOM), neutral detergent fiber (NDF), and crude protein (CP). Cycle means (g kg^–1 DM basis combined over both harvest dates) for IVDOM, NDF, and CP were 497, 810, and 142; 503, 797, and 137; 528, 787, and 132; and 520, 785, and 129 for C0, C4, C9, and C23, respectively. The average IVDOM of C4 was greater than for C0 (P = 0.03) and that for C9 was greater than for C4 (P < 0.001). Results indicated that forage quality also increased with advancing selection cycles for increasing yield.     

Germplasm Sources for Improvement of Forage Quality in Lotus corniculatus L. and L. uliginosus Schkuhr (Fabaceae)

Extensive germplasm resources are present in collections of Lotus corniculatus and L. uliginosus, two forage species that are valuable in situations where other legumes are not persistent. Naturalised populations in many regions are the primary gene pool for genetic improvement of these species and interspecific hybrids are an important source of variation for agronomic and forage quality traits. Although previous research has provided evidence that selection for optimal levels of condensed tannins and herbage productivity are feasible breeding objectives, less is known about correlations of condensed tannin with other parameters of nutritive value. A germplasm collection of 38 accessions in both species from 9 geographic regions was used as a data base to determine these correlations. Forage of each accession was sampled in two successive years from a field site in south-eastern Australia and analysed for condensed tannin, in vitro digestible dry matter, nitrogen, acid detergent fibre and neutral detergent fibre. In both species condensed tannin was negatively correlated with in vitro digestible dry matter and nitrogen. This result helped to identify potentially valuable accessions with low condensed tannin (<4% of dry weight) and high in vitro digestible dry matter (>70%). One of these was an interspecific hybrid of L. uliginosus and L. corniculatus, confirming the view that interspecific hybridization would be a valuable avenue for improvement of agronomic and forage quality characters in Lotus species.     

Predicting silage maize yield and quality in Sweden as influenced by climate change and variability

Abstract

In recent decades European silage maize production has extended northwards, into Scandinavia, and the importance of maize in fodder production has increased substantially. For the northward expansion of maize production it is of interest to evaluate both the role of climate change that has occurred already, and scenarios for possible future climate change. The aim of this study was to assess for Swedish climatic conditions, the annual variation in silage maize yield and quality (dry weight and starch contents) of cultivars currently grown in Germany.

The MAISPROQ simulation model currently used in German maize production was applied to evaluate the effects of (i) cultivar differences (four cultivars; four sites; 2003–2009), (ii) intra-regional variation among ten sites representing three regions (two cultivars; 2003–2009), and (iii) climatic variability among two historical periods during 1961–2009 and three future periods during 2011–2100 using A2-emission climate scenarios and the Delta-method (two cultivars; four sites).

Forage quality assessments strongly influenced the assessments of harvest time and thereby the yield. Changes in simulated yield of the tested cultivars were high for the past climate, but relatively small under future climatic conditions due to earlier harvest caused by improved forage quality. By the end of the 21st century an appropriate fodder quality would be achieved every year in the south of Sweden, whereas in the middle of Sweden (60°N) about 30% of the years would not be successful, even for the earliest cultivar. In the east, increased water stress counteracted the positive effect of a prolonged growing season. It was concluded that adaptation of field experiments to model calibration requirements remains to be done, in order to enable extrapolation of observations from Swedish field trials to a changing future climate.     

Calcium,magnesium, and potassium uptake by crested wheatgrass grown on calcareous soils

Abstract

Forage intake with potassium/(calcium + magnesium) [K/(Mg + Ca)] values in excess of 2.2 are associated with grass tetany and Mg deficiencies in ruminants. This study was conducted to determine the degree to which forage K and Mg concentrations and K/(Ca + Mg) ratios could be predicted from soil bicarbonate (HCO₃) extractable phosphate‐phosphorus (PO₄‐P), and saturation extract Ca, Mg, K, sodium (Na), and nitrate‐nitrogen (NO₃‐N) concentrations. Crested wheatgrass (Agropyron spp) strains and cultivars representing four ploidy levels were grown in the greenhouse on eight calcareous soils with different saturation extract Ca, Mg, K and K/Mg ratios. The plants were harvested three times. Soil solution K/(Ca + Mg) and K/Mg ratios were the only measured soil parameters that showed a consistent correlation with plant K/(Ca + Mg) ratios. Bicarbonate extractable soil P was positively related to plant P and K uptake in the first harvest, but was not related in the second and third harvests nor was soil P related to plant Ca or Mg content. There was a tendency for the higher ploidy level entries to have higher plant K/(Ca + Mg) ratios. It was concluded that soil K/(Ca + Mg) ratios can be used to predict relative forage K/(Ca + Mg) ratios for grasses grown under similar conditions.     

Response of texas bluegrass to season of nitrogen fertilization on the Louisiana coastal plain

Texas bluegrass (Poa arachnifera Torr.) has shown potential for use as a cool‐season perennial pasture grass in the southern Great Plains, where it occurs as a natural component of rangeland plant communities, and into the western Coastal Plain. Responsiveness of this grass to nitrogen (N) fertilization appeared to be limited to the spring growing period in initial evaluations in Louisiana. A field plot experiment was conducted to assess forage production and quality responses to season of N fertilization on the Syn‐1 population of Texas bluegrass. Winter forage production responses to 50 kg N ha^‐1 were obtained in the 1997–98 growing season but not in 1998–99 after stands had been depleted by summer drought. Greatest yield increases resulted from spring N application, however, fall plus winter fertilization provided the most uniform distribution of forage through the cool season. Forage fiber fractions, in vitro digestibility, and crude protein were not affected by N fertilization. Both amount and distribution of Texas bluegrass forage, but not forage quality, can be manipulated by time of N fertilization.     

Nitrogen efficiency in hydroponic chicory

An experiment was conducted to develop a model of vegetative growth based on the nitrate concentrations in nutrient solutions using the nutrient film technique and to determine the best cultivar for each concentration. The chicory cultivars (“Pão de Açúcar,” “Folha Larga,” “Precoce de Trieste” and “Catalonha”) and the subplots corresponded to different nitrate concentrations (6.68, 10.0, 13.32 and 16.64 mmol L^?1). There was an interaction between the evaluated factors, such that there was no similarity in the response of the chicory cultivars to the N concentrations in the nutrient solution. The most productive chicory cultivar was “Pão de Açúcar,” which exhibited 10.5% higher productivity than the average, and the least productive was “Folha Larga,” which showed approximately 8.5% lower productivity than the average.     

Bread Quality of Spelt Wheat and Its Starch

Flours from five spelt cultivars grown over three years were evaluated as to their breadbaking quality and isolated starch properties. The starch properties included amylose contents, gelatinization temperatures (differential scanning calorimetry), granule size distributions, and pasting properties. Milled flour showed highly variable protein content and was higher than hard winter wheat, with short dough‐mix times indicating weak gluten. High protein cultivars gave good crumb scores, some of which surpassed the HRW baking control. Loaf volume was correlated to protein and all spelt cultivars were at least 9–51% lower than the HRW control. Isolated starch properties revealed an increase in amylose in the spelt starches of 2–21% over the hard red winter wheat (HRW) control. Negative correlations were observed for the large A‐type granules to bread crumb score, amylose level, and final pasting viscosity for cultivars grown in year 1999 and to pasting temperature in 1998 samples. Positive correlations were found for the small B‐ and C‐type granules relative to crumb score, loaf volume, amylose, and RVA final pasting viscosity for cultivars grown in 1999, and to RVA pasting temperature for samples grown in 1998. The environmental impact on spelt properties seemed to have a greater effect than genetic control.     

Grass species and cultivar differences in magnesium concentration

Abstract

Two rates of Mg, 0 and 56 kg/ha, were randomized within each of 43 cultivars that had previously been established in a randomized complete block design. The 43 cultivars consisted of 6 species. Forage samples were obtained in early spring and chemically analyzed for content of Ca, Mg and K.

Grass species and cultivars with species significantly differed in content of Ca, Mg and K and in the ratio meq K/(meq Ca + meq Mg). Significant interactions of Mg with species and Mg with cultivars within species were observed. At the relatively low level of soil Mg and Mg rate, no cultivar contained 0.20% Mg, a value often used to identify proneness to grass tetany. However, no cultivar had an average meq ratio, K/(Ca + Mg), greater than 2.2, a value often used in diagnosing potential tetany problems that might be associated with intake of low Mg forage.

The interaction of cultivar with Mg rate indicates that fertilizer Mg level influenced cultivar content of Mg, and that the effect was not consistent for all cultivars.     

Potential of feed-food double-cropping in central highlands of Ethiopia

The potential of feed-food double-cropping was evaluated at Ginchi, Ethiopia, for two years with the objective of evaluating herbage yields of fodder crops and the subsequent effects on grain yields of chickpea and grass pea. Early maturing oat (79Ab382 (TX) (80SA95); 79CP84 (Coker SR. res) 80SA130; SRCP X 80Ab2806; C7512/SRCP X 80Ab 2252 and CO X SRCP X 80Ab2291) and common vetch (Acc. No. 2490; Acc. No. 2742) were selected and planted on lands reserved for chickpea/grass pea at the start of the main rainy season. Following forage harvest, chickpea and grass pea were planted on all plots and control fallow plot (farmers’ practice). A randomized complete block design with three replicates was used. Vigor (potential growth), plant height, herbage yield of forage crops, and grain yields of chickpea and grass pea varied across years. Higher average herbage yield (3.36 t ha^–1) was obtained from oat–common vetch mixtures compared to a fallow system (1.11 t ha^–1). Moreover, grain yields of chickpea and grass pea grown as a double-crop following oat–common vetch were more or less similar to yields obtained under a fallow system except in the case of chickpea following T6 (oat SRCP X 80Ab2806 × Vicia sativa acc. No. 2742). From this study, it was concluded that double-cropping of early-maturing, improved forage crops and residual soil moisture-based planting of chickpea and grass pea could improve feed availability, and labor and land productivity.     

Genetic variation for Mg tissue concentration in pearl millet lines grown under Mg stress conditions

Efficient nutrient utilization by plants is extremely important in developing countries and is becoming more important in the developed countries as the costs of fertilizers are increasing. Pearl millet is a world food and forage crop usually grown on droughty soils low in nutrients. Eighty‐five genetically diverse pearl millet, Pennisetum glaucum (L.) R. Br. lines were grown in low Mg and K sand for three weeks to screen for efficient Mg uptake. Magnesium free Hoagland's solution at one‐fourth strength was used to water the plants as needed. Forage produced was clipped, dried, ground, ashed, and analyzed for Mg and K content. A 2‐fold difference for Mg concentration in the forage existed among the lines. The data indicated that a low [K]/[Mg] ratio in the plant was indicative of a Mg efficient genotype. The data also suggest that caution should be used in making general nutrient recommendations based on tissue or soil tests without knowing the nutrient requirement of the cultivar.     

Exploration of genetic diversity within Cichoriumendivia and Cichorium intybus with focus on the gene pool of industrial chicory

The present study used 15 simple sequence repeat loci to characterize the genetic diversity of the germplasm that originated the current industrial chicory and to establish the relationships between and inside Cichoriumintybus L. and Cichorium endivia L. Initially we analyzed 19 cultivated C. endivia accessions, 27 wild and 155 cultivated C. intybus accessions distributed among three groups: 83 root chicories, 42 Witloof and 30 leaf chicories. The leaf chicories comprised cultivars corresponding to the Radicchio, Sugarloaf and Catalogne subgroups. The latter has not been previously included in any genetic diversity study. Subsequently, 1297 individuals from the 15 modern root chicory cultivars at the origin of the breeding of the current industrial root chicory cultivars were analyzed. Although the accessions of C. endivia and C. intybus were clearly separated from each other, seven wild C. intybus individuals were genetically closer to C. endivia than to C. intybus, revealing complex genetic interrelationships between these species. The differentiation of C. intybus into three cultivar groups (Witloof, root chicory and leaf chicory) was confirmed. The leaf chicory individuals were divided into three genetic subgroups, corresponding to the Radicchio, Sugarloaf and Catalogne cultivars, thus attesting to the validity of the classification based on morphological factors. Clear differentiation was observed among the Belgian, Polish and Austrian modern industrial root cultivars, but not among the French industrial modern root cultivars. The high phenotypic and genetic variability of the modern industrial root cultivars indicates that this germplasm constitutes a useful gene pool for cultivar improvement and selection.     

Declines in forage availability for bumblebees at a national scale

We assessed national scale changes in the forage plants of bumblebees in Britain, as a means of providing quantitative evidence for the likely principal cause of declines in bumblebee species. We quantified the relative value of native and long-established plant species as forage (nectar and pollen) resources for bumblebees by collating visitation data from 14 field sites across Britain. Twentieth Century changes in range and frequency of these forage plants were assessed using data from the New Atlas of the British and Irish Flora (1930-1969 to 1987-1999) and the Countryside Surveys of Britain (1978-1998). Forage plants declined in both large-scale range and local-scale frequency between the two survey periods. These changes were of greater magnitude than changes in other native plant species, reflecting serious reductions in quality of foraging habitats for bees as well as a general decline in insect-pollinated plants. Seventy-six percent of forage plants declined in frequency within 1-km squares, including those (e.g. Trifolium pratense) of particular value for threatened bumblebee species. We consider how our findings relate to other recorded changes in the British flora, how they may help to explain declines in bumblebees and how they could contribute to a conservation strategy.     

Forage nutrient composition in the wet season in Benue state,Nigeria. I. Macrominerals and in vitro organic matter digestibility

Abstract

Seventy‐one forage samples were analyzed for their macromineral composition and forage in vitro organic matter digestibility (IVOMD) in three agricultural zones of Benue State, Nigeria. The zones consisted of Northern, Eastern, and Central zones, each of which were made up of four Local Government Areas. In each Local Government Area, grazing animals were monitored and forages corresponding to those consumed were collected during the peak of the wet season (June) and analyzed for their macromineral composition and IVOMD. Fewer number of forage samples were harvested from Northern and Eastern zones due to greater agricultural activities and infra‐structural land use which limited land space for grazing animals. Forage IVOMD ranged from 32% to 78.3% across all classes of forage and from 43.5% to 62.8% in the Local Government Areas. About 18% of total samples, exclusively from the Northern zone, showed forage IVOMD concentrations below 20%, probably resulting from grazing pressures and agricultural activities in the zone. Forages from the Northern zone were deficient in phosphorus (P), potassium (K), and sodium (Na) based on their criteria of adequacy. No deficiency levels were indicated in forage calcium (Ca) or magnesium (Mg). Northern and Eastern zones were in greatest need of mineral supplementation programs.