干旱胁迫对黄土高原不同质地土壤长柄扁桃和沙柳幼苗生理生态特征的影响

通过盆栽控水试验,研究了干旱胁迫对黄土高原不同质地土壤(砂土、壤土)长柄扁桃和沙柳幼苗光合特性(净光合速率P_n、气孔导度G_s、胞间CO_2浓度C_i、内禀水分利用效率WUE)、蒸腾速率T_r及叶片水势Ψ_w的影响。结果表明:干旱胁迫下不同质地土壤长柄扁桃和沙柳P_n、G_s、WUE及T_r均呈现先上升后下降的趋势,而C_i和Ψ_w分别表现为上升和下降趋势。土壤质地显著影响长柄扁桃和沙柳对水分亏缺的敏感性,在相同干旱程度下,壤土中长柄扁桃P_n和G_s均显著高于砂土,而沙柳P_n和G_s表现为砂土显著高于壤土(p0.05)(重度干旱除外)。当土壤含水量降至中度和重度干旱时,长柄扁桃和沙柳WUE在不同质地土壤间具有显著差异(p0.05)。壤土中长柄扁桃生长优于砂土,而沙柳则相反。因此,黄土高原植被恢复与生态建设过程中不仅应根据土壤水分状况选择造林树种,还应考虑植物在不同质地土壤上对干旱胁迫的不同响应。     

土壤质地对玉米不同生理指标水分有效性的影响

为确定土壤质地对玉米不同生理指标水分有效性的影响,该文利用3种土壤（重壤土、中壤土和砂壤土）的盆栽控水试验和1种土壤（重壤土）的田间小区控水试验,研究了玉米不同生理指标随相对土壤含水率（土壤含水率占田间持水率的比）的动态变化。结果表明：3种土壤中各生理指标相对值在相对土壤含水率降低到土壤水分阈值之前保持不变,低于此阈值时随相对含水率的进一步降低而线性降低,且均可用分段函数来拟合（R2=0.824～0.999）。土壤水分有效性大小排序为：砂壤土＞中壤土＞重壤土,而且瞬时生理指标的土壤水分阈值低于日变化和整个试验阶段的累积指标。因此土壤质地和不同生理指标的时间尺度都会影响玉米生理指标对土壤水分有效性的响应。     

施磷对不同质地棉田土壤磷素有效性及磷肥利用率的影响

为阐明施磷对不同质地棉田土壤磷素有效性及磷肥利用率的影响,以盆栽试验为基础,在不同质地(粘土、壤土、砂土)上设计5个磷素水平(P0、P150、P300、P600、P1200)研究棉田磷素状况和棉花磷素积累及磷肥利用率。结果表明:不同质地棉田土壤有效磷含量在苗期和蕾期均随施磷量的增加而增加,苗期时粘土、壤土、砂土的土壤有效磷含量在P1200处理下与对照相比分别增加了80.94%、85.78%、94.41%,蕾期则分别增加了76.82%、85.10%、94.20%。苗期时,土壤全磷含量分别在粘土P600、壤土P1200、砂土P600处理下达到最大值;蕾期时粘土、壤土和砂土的全磷含量均在P1200处理达到最大值,土壤磷素活化系数在苗期时表现为粘土砂土壤土,蕾期磷素活化系数在粘土和砂土基本呈持续递增状态,最大值与对照(P0)相比分别增加了34.22%、85.71%。植物整株干物质积累在不同土壤质地表现为粘土砂土壤土。植物全磷含量则是壤土略低于粘土,砂土最低。棉花整株磷素积累量在不同土质上表现为粘土最高,壤土次之,砂土最低,且分别在P600、P300、P600处理时达到最大值。不同磷水平下,磷肥表观利用率在3种土壤质地上表现不同,粘土、壤土、砂土分别在P150、P300、P600时达到最大值,与P0相比分别提高了16.84%、29.19%、10.68%。同一磷水平下不同土壤质地磷素生理利用率表现为砂土壤土粘土。因此,在生产中应针对土壤质地合理施磷,粘土土质下棉田施磷量应控制在约150 kg/hm~2,壤土土质应控制在150～300 kg/hm~2,砂土土质施磷量总体应控制在300～600 kg/hm~2,才能促进土壤中磷的有效性和棉花磷素吸收,从而提高磷肥利用率。     

基于Hydrus-1D模型的玉米根系吸水影响因素分析

为探索土壤质地、植物生长状况和气象条件对不同土壤水分条件下根系吸水速率的影响机理,该文以相对根吸水速率与土壤含水率的关系衡量土壤水分有效性,利用Hydrus-1D模型模拟了3种土壤（壤黏土、黏壤土和砂壤土）中不同玉米生长状况（包括叶面积指数、根系深度和根系剖面分布）或蒸发力条件下根系吸水速率随含水率的动态变化,确定了不同条件下根系吸水速率开始降低的临界含水率。结果表明：土壤质地、植物的叶面积指数和根系分布及大气蒸发力都对根系吸水动态曲线的临界含水率有一定影响,其中根系深度和根系分布形状还影响根系吸水速率与含水率关系曲线的形状,但在3种土壤中,根系吸水速率的动态变化对植物生长和大气蒸发力的响应不同。总体而言,3种土壤临界含水率的大小是壤黏土＞黏壤土＞砂壤土;临界含水率随大气蒸发力的升高而升高,随根系深度和深层根系分布的增加而降低;各因子对玉米根系吸水影响程度的大小是土壤质地＞根系分布形状＞根系深度＞大气蒸发力＞叶面积指数。     

黄土高原土壤水分性质及其分区

黄土高原土壤具有很好的调节作物供水的功能,这和土壤的水分性质有关。本文研究了土壤的持水性能、有效性能和移动性能等。影响黄土高原土壤水分性质的主要因素是土壤质地。文内绘制了包括五个质地带的土壤质地分区图。田间持水量在轻壤土、中壤土和重壤土范围内均为20%±2,未表现出明显相关。萎蔫湿度则几乎完全决定于土壤质地。文内绘制了田间持水量和萎蔫湿度的等值线图。土壤水物理蒸发影响深度可达2-3米。两米土层内物理蒸发失水量轻壤土和中壤土达田间持水量的25-35%;具有下伏粘化层的重壤土,60厘米以下矢水量不大于10%。后者为作物准备了较多的储水。文内列出了不同质地土壤的水分特征曲线的经验方程,所有资料汇总于土壤水分性质表中。本研究结果为合理利用土地提供了基础数据。     

运用染色示踪方法研究土壤质地对土壤水非均匀流动特征的影响

为研究土壤质地对土壤水流运动非均匀特征的影响,在砂土、壤土和粉质粘土中采用亮蓝(砂土)和碘离子(壤土和粉质粘土)作为示踪剂分别开展了2个、5个和4个不同入渗条件(土壤初始含水率和入渗水量)的染色示踪试验,通过计算活动流场模型(Active Region Model,ARM)分形特征参数(γ)来定量描述和比较不同入渗条件下土壤水流运动的非均匀特征。研究结果表明,(1)活动流场模型较好的捕捉到了土壤水流运动整体的非均匀信息;(2)当其它入渗条件(土壤初始含水率和入渗水量)相同时,土壤水在细质地土壤中运动的非均匀程度要高于其在粗质地土壤中运动的非均匀程度。     

盐分对棉花光合特性及根际土壤CO2浓度的影响

为了探寻不同盐分含量土壤棉花生长根际CO2浓度与光合指标之间的关系,通过桶栽试验,对4种盐分(CK:0%,F1:0.2%,F2:0.4%,F3:0.6%)处理下2种质地(砂土和壤土)土壤CO2浓度和棉花光合特征的变化规律进行了研究。结果表明:随着棉花生育期的推进,壤土和砂土的土壤CO2浓度均呈先升高后降低的"单峰"曲线变化趋势,峰值出现在花铃期,分别高达17 061.95,17 572.00μmol/mol。在盐分处理下不同质地土壤CO2浓度随土层深度的增加而增加,50cm处土壤CO2浓度均值为13 540.32μmol/mol,是表层10cm处的近2倍。随着盐分含量的增加,2种质地土壤CO2浓度差异显著,均呈下降趋势,且壤土CO2浓度明显高于砂土;盐分和土壤质地类型的相互作用对棉花净光合速率(Pn)达到极显著水平(P0.01)。同一土壤质地类型条件下,各生育期棉花Pn随着盐分含量的增加而减小,均在F3处理下达到最小值,盐分含量较低时对棉花光合指标的影响不显著(P0.05);同一盐分处理下,不同土壤质地棉花Pn差异显著,表现为壤土砂土。不同盐分处理下2种质地土壤CO2浓度与棉花净光合速率之间密切相关,棉花Pn能够解释根际土壤CO2浓度变化的81.2%,说明盐分和土壤质地类型通过棉花净光合速率影响土壤CO2浓度的大小。研究结果可为作物生长环境提供理论参考。     

不同质地耕层土壤有效态微量元素含量特征

[目的]研究不同土壤质地下耕层土壤有效态微量元素含量特征,为合理制定农田土壤施肥方案和提高土壤养分资源利用率提供依据。[方法]以库车县不同质地耕层土壤(0—20cm)为调查对象,采用统计方法对土壤微量元素有效态含量特征进行分析。[结果](1)土壤有效态微量元素在壤土、砂壤土、黏土、黏壤土及砂土中含量差异显著(p0.05),且壤土和砂壤土的有效态微量元素含量相对较高;(2)土壤微量元素有效性综合指数排列顺序依次为:砂壤土(1.51)砂土(1.44)黏土(1.42)壤土(1.41)黏壤土(1.27);(3)土壤有机质与土壤有效态微量元素均具有极其显著的相关性(p0.01),pH值则与有效铜和有效锰相关显著(p0.05)。[结论]在不同土壤质地下,微量元素铁和锌含量较为缺乏,锰和铜含量则相对较为丰富,故应依据这一特性进行土地科学管理和施肥。     

一维马尔可夫链模拟黑河中游流域土壤质地垂向变异

黑河中游地区土壤剖面砂、黏层次相间排列的特点及部分区域剖面中出现的不透水层对土壤中的水分运动和溶质迁移具有重要影响。该研究调查了黑河中游100km2区域内土壤剖面的质地分层情况,运用一维嵌入马尔可夫链模型模拟该地区的土壤质地剖面。研究表明,研究区土壤剖面共出现砂土、壤质砂土、砂质壤土、壤土、黏质壤土、粉黏壤土和粉黏土7种质地类型,层次厚度呈对数正态分布。粉黏土未在表层土壤出现,而砂土在表层出现的概率明显高于其余质地类型,剖面某一质地层之下多出现细粒含量比其高的下一质地类型。剖面上相邻两质地层之间的转移具有明显的马尔可夫链特征(简称马氏性),且马氏链是平稳的。一维嵌入马尔可夫链模型能够较好地描述研究区土壤质地层次的垂向变化,剖面主要的质地层次组合为:砂—壤、壤—砂、壤—黏、黏—壤。对土壤质地剖面的定量模拟,可以为开展该区域土壤水循环、转化和溶质迁移等相关研究提供参考。     

利用植物气体交换参数确定萎蔫系数的方法

萎蔫系数是确定土壤有效水范围、储量和对植物有效性的关键因子,但现有的基于植物形态变化测定的萎蔫系数存在生理意义不明确及难以在田间原位测定的缺陷。为此,本研究利用自然干旱下的盆栽试验,测定了2种土壤质地（黄土高原区黄绵土和南方丘陵区红壤）下4种植物（大豆Glycine max L.、向日葵Helianthus annuus L.、苜蓿Medicago sativa L.、羊草Leymus chinensis (Trin.) Tzvel）幼苗的萎蔫系数及叶片气体交换参数对土壤水分含量变化的动态响应过程,探讨了基于植物气体交换参数确定的土壤水分下限阈值与萎蔫系数的关系。结果表明：（1）土壤质地和植物抗旱性显著影响萎蔫系数,且影响在永久萎蔫时对应的土壤水势。4种植物在黄绵土下的萎蔫系数分别为0.083 cm3?cm-3（向日葵）>0.081 cm3?cm-3（大豆）>0.072 cm3?cm-3（羊草）>0.060 cm3?cm-3（苜蓿）,在红壤下的萎蔫系数表现为0.188 cm3?cm-3（向日葵）>0.180 cm3?cm-3（大豆）>0.174 cm3?cm-3（羊草）>0.172 cm3?cm-3（苜蓿）。4种植物在红壤上的萎蔫系数均大于黄绵土,且植物抗旱性越强,其萎蔫系数越低。（2）利用三次函数模拟气孔导度变化确定的土壤水分下限阈值与萎蔫系数存在高度一致性。因此,萎蔫系数可基于植物气体交换参数进行间接估算。     

Agricultural potential of Mediterranean grain and forage legumes: Key differences between and within Vicia species in terms of phenology, yield, and agronomy give insight into plant adaptation to semi-arid environments

In order to identify key adaptive traits which affect productivity in Mediterranean grain and forage legumes and simultaneously determine the agricultural potential of a wide range of Vicia species, germplasm collected from the wild throughout the eastern Mediterranean was grown under semi-arid conditions in Tel Hadya, Syria (313 mm growing season rainfall). These included species currently in use in Mediterranean agriculture, such as V. sativa L., as well as those more widely used in the past-such as V. ervilia L., but also a broad selection from Section Narbonensis (B. Fedtsch. ex Radzhi) Maxted, including V. narbonensis L, V. johannis Tamamsch., V. hyaeniscyamus Mout., V. serratifolia Jacq., and V. kalakhensis Khattab et al. V. faba, a near relative of the taxa in Section Narbonensis, was included as a domesticated control. Where applicable, a representative range of subspecies was used. Accessions were chosen from a wide range of habitats in terms of latitude (31.02–40.72 decimal^o), longitude (27.1–43.17 decimal^o), altitude (20–1510 m), rainfall (180–1700 mm/yr) and soil depth (5–50 cm) in order to maximise diversity within species. Agricultural potential was determined by measuring seed, hay and biological yield, as well as agronomic traits such as harvest index, standing crop height, and seed size. The comparative influence of phenology and key agronomic traits such as plant habit and seed size on productivity varied tremendously between species, depending on their reproductive strategies. In V. sativa and V. ervilia, the smaller seed species which rely on long vegetative phases and growing seasons to accumulate sufficient biomass to set seed, and in which there was comparatively little agronomic variation, phenology had a large impact on yield. In early emerging taxa such as V. ervilia and V. s. subsp. sativa, with built-in long vegetative phases and growing seasons, seed yield was negatively correlated with flower ing (r = –0.86 to –0.88), whereas the opposite was the case for later emerging taxa such as V. s. subsp. nigra (L.) Ehrh. (r = 0.95). Within V. narbonensis and relatives, the larger seeded Vicia species which rely on more conservative reproductive strategies where high seedling vigour associated with large seeds enables the species to enter reproductive phases relatively early, phenology had a much smaller impact on yield than did variation of key traits such as seed weight, plant habit and pod shattering. Among the undomesticated germplasm harvest indices ranged from 0.09–0.31, hay yields from 0.1–3.4 t/ha, seed yield from 0–2.0 t/ha, and dry matter at maturity from 1.6–6.5 t/ha. Sub-specific taxonomy was crucial in determining agronomic potential. V. narbonensis var. aegyptiaca Kornhuber ex Asch. et Schweinf. showed the most potential, combining an upright habit, large seeds (212 mg) and tendency to retain intact pods after maturity, with the highest yield, harvest index and crop height of all the wild Vicia species. V. sativa subsp. sativa, V. ervilia and V. narbonensis var. narbonensis were less productive, but still showed agricultural potential. The smaller seeded V. narbonensis, var. affinis, var. jordanica H. Schäf. and var. salmonea (Mout.) H. Schäf., and their close relatives V. johannis, V. hyaeniscyamus, V. serratifolia and V. kalakhensis have little to offer Mediterranean agriculture on the basis of poor agronomy.     

On the Taxonomy of the Members of ‘Saccharum Complex’

The difficulty and problems encountered in the study of cultivated plants, in general and sugarcane, in particular has been indicated. In order to understand these problems, a brief review on the taxonomy of Saccharum and closely related taxa, namely, Erianthus, Sclerostachya, Narenga and Miscanthus (generally known as ‘Saccharum complex’) has been given. A short account on the important morphological features that are specific to sugarcane has also been stated as classification is commonly based on such morphological characters. A note has been added on the chromosome number, origin, and distribution of the species of ‘Saccharum complex’ members. Taxonomic keys have been devised for identification of the genera of Saccharinae and for the species of Saccharum and Erianthus occurring in India. A new combination, Sclerostachya fallax (Balansa) Amalraj et Balasundaram, has also been proposed.     

Yield and N assimilation of winter wheat (Triticum aestivum L., var. Norstar) inoculated with rhizobacteria

The effects of bacterial inoculants on the growth of winter wheat were studied in a growth chamber. Azospirillum brasilense, Azotobacter chroococcum, Bacillus polymyxa, Enterobacter cloacae, or a mixture of the four rhizobacteria were the inoculants tested. Inoculation effects on yield, yield components, and N-derived from fertilizer (Ndff) were assessed. The response of plants inoculated with individual bacteria was inconsistent and varied with treatment. At the first harvest (58 days after planting-DAP) plants inoculated with the mixture exhibited increases in plant dry weight, total-N and Ndff. At the second harvest (105 DAP), plants inoculated with A. brasilense and the mixture exhibited increases in shoot biomass, whereas at maturity (170 DAP), the inoculated plants showed no differences in total-N or shoot dry matter yield, as compared to the uninoculated controls. Inoculation with A. brasilense, however, increased the Ndff in the shoots, and B. polymyxa tended to enhance grain yield. Practical use of these rhizobacteria as inoculants for winter wheat may have limited value until such time as we better understand factors which influence rhizosphere competence of bacterial inoculants.     

Polymorphism of high molecular weight glutenin subunits in three species of Aegilops

A collection of 136 accessions of Aegilops umbellulata (39), Ae. comosa (75) and Ae. markgrafii (22) was analysed for high-molecular-weight (HMW) glutenin subunits composition. The homogeneity of the accessions was studied and 55.1% of the collection was homogeneous for HMW glutenin subunits (29 Ae. umbellulata, 33 Ae. comosa and 14 Ae. markgrafii). The HMW glutenin subunits of Ae. umbellulata are encoded by the Glu-U1 locus; in Ae. comosa results showed that this proteins are encoded at the 1M chromosome, and the locus was named Glu-M1. In Ae. markgrafii it was assumed that HMW glutenin subunits were encoded by an homoeologous locus and it was named Glu-C1. All the accessions of Ae. umbellulata and Ae. markgrafii expressed both, x-type and y-type subunits. Among the Ae. comosa accessions, only one expressed an x-type subunit alone. All the accessions of Ae. umbellulata and some of Ae. comosa had x-type glutenins of higher molecular weights than those commonly present in bread wheat. A total of 8 alleles were detected at the Glu-U1 locus, 11 at the Glu-M1 and 4 at the Glu-C1. The new HMW glutenin variation found in this work suggests their possible utilisation in breeding for wheat quality.     

Genetic diversity of rhizobia associated with indigenous legumes in different regions of Flanders (Belgium)

We investigated the diversity of rhizobia isolated from different indigenous legumes in Flanders (Belgium). A total of 3810 bacterial strains were analysed originating from 43 plant species. Based on rep-PCR clustering, 16S rRNA gene and recA gene sequence analysis, these isolates belonged to Bradyrhizobium, Ensifer (Sinorhizobium), Mesorhizobium and Rhizobium. Of the genera encountered, Rhizobium was the most abundant (62%) and especially the species Rhizobiumleguminosarum, followed by Ensifer (19%), Bradyrhizobium (14%) and finally Mesorhizobium (5%). For two rep-clusters only low similarity values with other genera were found for both the 16S rRNA and recA genes, suggesting that these may represent a new genus with close relationship to Rhodopseudomonas and Bradyrhizobium. Primers for the symbiotic genes nodC and nifH were optimized and a phylogenetic sequence analysis revealed the presence of different symbiovars including genistearum, glycinearum, loti, meliloti, officinalis, trifolii and viciae. Moreover, three new nodC types were assigned to strains originating from Ononis, Robinia and Wisteria, respectively. Discriminant and MANOVA analysis confirmed the correlation of symbiosis genes with certain bacterial genera and less with the host plant. Multiple symbiovars can be present within the same host plant, suggesting the promiscuity of these plants. Moreover, the ecoregion did not contribute to the separation of the bacterial endosymbionts. Our results reveal a large diversity of rhizobia associated with indigenous legumes in Flanders. Most of the legumes harboured more than one rhizobial endosymbiont in their root nodules indicating the importance of including sufficient isolates per plant in diversity studies.     

Changes in trace element speciation in kola north surface waters during snow melt

The powerful mass-flow of melt water during the brief period of spring thaw provides a surge of pollutants and causes extremely toxic short term conditions. Together with the increase in total metal concentrations during this period, a redistribution in metal speciation occurs that increases the proportion of more toxic forms. Concentrations of ion-exchangeable fractions of nickel (Ni), copper (Cu), aluminium (Al), and manganese (Mn) show strong negative correlations with pH. The sum of complex metal species shows a strong positive correlation with total organic carbon (TOC). A data analysis model based on relationships observed between key parameters and metal behaviour together with gidrological data allowed an assessmrnt of the metal impact on waterbodies from accumulated airborne pollution (Ni, Cu) as well as from accelerated leaching from watersheds (Al, Mn).     

The effects of feeding by Oniscus asellus on leaf litter sulfur constituents

Summary Feeding by the isopod, Oniscus asellus, produced changes in the sulfur constituents of leaf litter substrates (Acer negundo, A. saccharum, Fagus grandifolia, Picea rubens, and Tsuga canadensis). Isopod consumption of leaf litter generally accelerated the mineralization of carbon-bonded S and increased the formation of ester sulfate in all substrates. After the isopod egestion of A. negundo leaves, fecal decomposition over 6 weeks increased total S concentration from 68 to 120 mol S/g due to the catabolism of organic carbon. During the same period sulfate decreased from 34 to 20 mol S/g and carbon-bonded S increased from 34 to 100 mol S/g. Thus the total S pool in aged feces became enriched with organic S (83% of total S). Macroinvertebrate consumption accelerated the transformation of S constituents and the carbon-bonded S concentration approached that of the Oa organic horizon of a northern hardwood forest.Dedicated to the late Prof. Dr. M.S. Ghilarov     

Phylogenetic relationships of Actinidia and related genera based on micromorphological characters of foliar trichomes

The phylogenetic relationships within the genus Actinidia and among 3 allied genera Clematoclethra, Saurauia, and Sladenia have been very controversial. In order to understand the systematic implication of foliar trichomes in those genera, the micromorphological characteristics of foliar trichomes from 34 taxa were examined by means of light microscopy and scanning electron microscopy (SEM). The observed micromorphological characteristics were classified into eight main types. A phylogenetic analysis of Actinidia and related genera based on 15 micromorphological characters of foliar trichomes was conducted using Wagner parsimony method and Sladenia celastrifolia, Rhododendron hybridum and R. simsii as a complex outgroup. In the resulting phylogenetic tree, Actinidia is shown to be a monophyletic group, and Clematoclethra and Saurauia form another monophyletic group. This study also presents the phylogenetic relationships among 4 sections within the genus Actinidia: the monophyly of sect. Leiocarpae and of sect. Strigosae, and polyphyly of sect. Maculatae and of sect. Stellatae.