Can field populations of the enchytraeid,Cognettia sphagnetorum,adapt to increased drought stress?

The ability to evolve increased drought tolerance in response to climate change was investigated in the enchytraeid, Cognettia sphagnetorum. Populations exposed to reduced precipitation or increased night time temperature for more than six years were collected in mixed Calluna/grass heathland at the Mols Laboratory, Denmark. The level of prolonged drought and increased temperature corresponded to a predicted climate change scenario and has been applied since 1999. In autumn 2005, enchytraeids were sampled in 3 cm intervals down to 9 cm depth and total number, biomass, diversity and soil organic matter were determined. The drought treatment resulted in a significant reduction of the density and biomass of enchytraeids, as well as changes in the species composition. In total, five different genera were found at the site in all three treatments (control, temperature and drought). C. sphagnetorum was the dominant species, especially in the upper 0–3 cm, and was clearly affected by the drought treatment. C. sphagnetorum from all plots were cultured in the laboratory to rear second or third generation adults. Results showed that populations of drought treated plots had not developed an increased drought resistance compared to populations of control or warming plots even after several years of a putative severe selection. Lack of adaptive potential in C. sphagnetorum suggests that more frequent periods with drought in the future will have a very strong negative influence on enchytraeid density, biomass and diversity.     

Involvement of Enchytraeidae (Oligochaeta) in soil structure evolution in agricultural fields

Summary The effects of enchytraeid activity on soil structure were recorded in a field study on population dynamics in agro-ecosystems and in a field experiment, using artificially compounded soil cores. It was established that 21–35% of the enchytraeid population contained mineral grains. The estimated transport of mineral material in the upper 0.4 m amounted to 0.001–0.01% of the bulk soil per year. In experimental cores with enchytraeids present, the air permeability, volume of pores in the size class corresponding to the enchytraeid body width, and the proportion of aggregates corresponding to the size of their fecal pellets were higher than in cores without enchytraeids.Dedicated to the late Prof. Dr. W. Kühnelt Communication no. 15 of the Dutch programme on soil ecology of arable farming systems     

Population dynamics of Enchytraeidae (Oligochaeta) in different agricultural systems

Organic matter and abiotic conditions seem to influence distribution patterns of Enchytraeidae. In this study effects of changes in management practices on population dynamics of enchytraeids were determined. At two sites (in Athens and Griffin, GA) parts of a fescue (FE) field were converted into conventional tillage (CT) and no-tillage (NT) plots and changes in densities and depth distribution of enchytraeids were followed for 3 years. A site at Watkinsville, containing various soil textures and characterised by very low organic carbon content, which was converted into no-tillage 4 years earlier, was also sampled. Significant reductions in enchytraeid densities, after conversion of fescue into CT, were only found at Griffin. The management practices affected the vertical distribution of enchytraeids. In fescue and NT more enchytraeids were found in the 0–5cm than in the 5–15cm layer. In conventional tillage fields enchytraeids were more evenly distributed over the profile or more abundant in the 5–15cm layer. Management also affected the timing of population dynamics in the different plots. At two sample dates high abundances were found in CT plots only, not in any of the other plots. Enchytraeids were larger at Athens than at Griffin and Watkinsville and contained more soil particles in their gut. At Athens enchytraeids will presumably contribute more to the development of soil structure than at the other sites. We conclude that management affects vertical enchytraeid distributions in soil and changes the timing of population dynamics. Received: 7 January 1996     

Increased frequency of drought reduces species richness of enchytraeid communities in both wet and dry heathland soils

Studies of biological responses in the terrestrial environment to rapid changes in climate have mostly been concerned with above-ground biota, whereas less is known of belowground organisms. The present study focuses on enchytraeids (Oligochaeta) of heathland ecosystems and how the enchytraeid community has responded to simulated climate change in a long-term field experiment. Either increased temperature or repeated drought was applied for 13 years to field plots located in Wales, The Netherlands and Denmark representing a gradient in precipitation and annual temperature fluctuations thereby providing an opportunity to study biological responses on a local (within sites) and regional scale. Warming treatments increasing night-time temperature (0.5–1 °C higher than ambient at 5 cm soil depth) had no detectable effects on the enchytraeid communities. Increased intensity and frequency of drought had rather weak persistent effects on total enchytraeid abundance suggesting that ecosystem functions of enchytraeids may only be transiently impacted by repeated spring or summer drought. However, drought treatment had persistent negative effects on species richness and community structure across sites. Drought treated plots harboured only 35–65% of the species present in control plots, and the reduction of species richness was most pronounced at the driest sites. It is discussed that soil invertebrates, due to their weak migratory potential, may be more liable to extinction under changing climatic conditions than above-ground species, and therefore consequences of climate change to soil organisms need particular attention in future research.     

Earthworm invasion alters enchytraeid community composition and individual biomass in northern hardwood forests of North America

European earthworms are invading many ecosystems worldwide and fundamentally transform habitats by acting as dominant ecosystem engineers. However, there is little knowledge of the consequences of earthworm invasion on the composition and diversity of native soil organisms. Particularly functionally similar groups, such as enchytraeids (Annelida: Enchytraeidae), may be affected through changes in the chemical and physical properties of the soil, but also due to competition for resources. In 2010–2011, we studied the impact of earthworm invasion on enchytraeids at two sites in the northern hardwood forests of North America: one site within the Chippewa National Forest in northern Minnesota and one site in the Chequamegon-Nicolet National Forest in northern Wisconsin, USA. At each site, three plots were sampled along a transect, representing (1) a non-invaded or very slightly invaded area, (2) the leading edge of earthworm invasion and (3) a heavily invaded area with an established population of the anecic earthworm Lumbricus terrestris (among other species). In total, 29 enchytraeid (morpho)species were identified (some yet to be formally described, several first or second records for the continent); of those 24 occurred at the Minnesota site and 17 at the Wisconsin site. The structure of enchytraeid assemblages differed significantly among the three invasion stages, although this was not equally pronounced at the two sites. Each stage was characterized by one or several indicator species. Mean enchytraeid densities (10,700–30,400 individuals/m²) did not differ significantly among the invasion stages, but were lowest at the leading edge of earthworm invasion at both sites. In the heavily invaded plot at the Minnesota site, the mean enchytraeid density and biomass in L. terrestris middens were significantly higher than in soil in-between the middens. This was due to a pronounced effect of L. terrestris middens in the uppermost 3 cm of soil. Differences in biomass among earthworm invasion stages were most apparent for mean individual biomass. This was significantly higher in the heavily invaded area than at the leading edge or in the non-invaded area at the Minnesota site. Compositional changes of the enchytraeid assemblage are likely to result in changes in the functioning of soil foods webs. Our results suggest that earthworm invasions can cause a loss of native species in soil, including heretofore unknown ones, that might go unnoticed.     

Tillage and drainage impact on soil quality: II. Tensile strength of aggregates, moisture retention and water infiltration

Tillage-induced changes in soil quality are important to understanding soil strength and water retention and transmission properties. Thus, this study was conducted to assess the effects of two tillage systems under un-drained and drained conditions on tensile strength (TS) of 5–8 mm aggregates, soil water characteristics (SWC), plant available water (PAW), and the water infiltration rate (i). Soil properties were determined mainly in the surface (0–10 cm) layer on a Crosby (fine, mixed, mesic, Aeric Ochraqualf) silt loam soil at the Waterman Farm of the Ohio State University, Columbus, OH on a 14-year-old field study. Effect of two tillage treatments comprising no-tillage (NT) and conventional tillage (CT) were studied for two levels of drainage: un-drained (UD) and tile drained (D). The TS for 0–10 cm depth was significantly (P ≤ 0.01) affected by tillage and drainage treatments, and was higher in CT than NT by 61% in UD and by 48% in D soil. In comparison, TS increased by 13% in NT and 4% in CT in D compared with the UD treatments. Soil organic carbon (SOC) in 0–10 cm depth of NT–UD treatment was 23% higher than CT–UD treatment and 38% more than NT–D treatments. Tillage and drainage impact on SWC was non-significant at 0 kPa suction, but significant (P ≤ 0.1) at −3, −6, −10, −30, −100 and −300 kPa suctions indicating that water was retained more in NT–UD than CT–UD soil. The PAW was significantly influenced by drainage (P ≤ 0.01) but not by tillage treatments. Yet, there existed a general trend of about 8% more PAW in NT–UD than CT–UD treatments. In contrast, PAW was 48% more in soil from NT–UD than NT–D treatments. PAW increased with increase in the SOC concentration (R² = 0.89; P ≤ 0.01). There were also differences in soil water sorptivity (S), and equilibrium infiltration rate (i_c) in NT–UD compared with CT–UD treatments. A positive and significant correlation (r = 0.57, P ≤ 0.05) occurred between i_c and SOC concentration. The value of S was more in NT–UD by 70% than CT–UD, and 46% in NT–D than CT–D. Similarly, the i_c was more in NT than CT by 119% in UD compared with 82% in D soil. The value of A in NT was higher than that in CT by 39% and 12% in UD and D treatments, respectively. The mean cumulative infiltration (I) in 3 h was 71.4 cm in NT versus 44.0 cm in CT in UD compared with 62.1 cm in NT and 48.4 cm in CT for the D treatment. The I was positively and significantly correlated with SOC concentration (r = 0.32, n = 12, P ≤ 0.1) indicating improvement of I with increase in SOC concentration. Results of this study suggest that conversion from CT to NT management system may reduce the risk of surface runoff, increase soil aggregation, and improve soil hydrological properties.     

Use of an instrumented disc coulter for mapping soil mechanical resistance

A soil mechanical resistance sensor with a large-diameter disc coulter was developed to delineate areas of differing soil strength across agricultural fields. The instrumented disc coulter consisted of a 76.2 cm disc with two depth-measuring sensors (rotary potentiometer and ultrasonic proximity sensor) along with a global positioning system (GPS) receiver to georeference operating depth measurements. The consistency and repeatability of the system response were evaluated by making six passes across long-term tillage comparison plots with different degrees of soil disturbance, including: 20 cm plowing, 15 cm disking, 30 cm chiseling, and no-till in several combinations. At the time of testing, standard soil cone penetrometer measurements were taken. The relationship between the average cone index in the 0–30 cm soil profile (CI_0–30 cm) and the disc operating depth was evaluated. In addition, the cumulative energy density of the given depth of penetration defined as specific cone penetration energy (J m⁻² or N cm⁻¹) for each tillage plot was calculated using the cone index profiles. The average measured depth in each tillage plot was compared to the average predicted depth (d_ci) of a fixed specific cone penetration energy (P_ci). Static calibration tests on the depth sensors showed excellent linearity with coefficients of determination (R²) greater than 0.99. The results showed that, on the average, the changes in the depth measured with the rotary potentiometer were 44 and 68% of the changes in the depth measured with the ultrasonic proximity sensor while the disc coulter was passing across, or along, the tillage plots. This difference was primarily due to the sinkage of the tractor wheels. The depth measured with the ultrasonic sensor had significant correlation with both CI_0–30 cm and d_ci. This was partially due to the fact that a significantly high correlation (R² = 0.97) between the CI_0–30 cm and d_ci was observed, which was not expected and originated from the type of soil profiles present. The instrumented disc coulter is a low soil disturbance system and could be used as an inexpensive and simple sensor to obtain information about the mechanical condition of the soil for spot tillage or other management decisions.     

Changes in soil organic carbon, nutrients and aggregation after conversion of native desert soil into irrigated arable land

This study aimed at investigating the effects of agricultural exploitation on desert soil organic C, N and P, and soil aggregation. Four land uses were assessed: (1) 5-year wheat (Triticum aestivum L.)/barley (Hordeum vulgare L.) + 5-year maize (Zea mays L.); (2) 5-year wheat/barley + 5-year alfalfa (Medicago sativa L.); (3) 6-year wheat/barley + 4-year acacia (Robinia pseudoacacia L.) and (4) uncultivated desert soil. The desert soil contained total organic C (TOC) of 3.1, 3.7 and 4.2 g kg⁻¹ and particulate organic C (POC) of 0.6, 0.7 and 0.8 g kg⁻¹ at 0–10, 10–20 and 20–30 cm depths, respectively. The soil TOC concentration was increased by 32–68% under wheat–maize rotation and by 27–136% under wheat–acacia at 0–20 cm depth, and by 48% under wheat–alfalfa only at 0–10 cm depth. This contrasted with an increase in the soil POC concentration by 143–167% at depth 0–20 cm under wheat–maize and by 217%, 550% at depth 0–10 cm under wheat–alfalfa and wheat–acacia, respectively. The desert soil had 13 Mg ha⁻¹ TOC stock and 2 Mg ha⁻¹ POC stock at depth 0–30 cm, whereas crop rotations increased the soil TOC stock by 30–65% and POC stock by 200–350%. Over the 10-year period, the rates of TOC accumulation were 0.6, 0.3, 0.8 Mg ha⁻¹ year⁻¹ and the rates of POC accumulation were 0.4, 0.4 and 0.7 Mg ha⁻¹ year⁻¹ under wheat–maize, wheat–alfalfa and wheat–acacia rotations, respectively. At 0–30 cm depth, total soil N was increased by 61–64% under wheat–maize and wheat–acacia, but total soil P was reduced by 38% under wheat–alfalfa. A significant improvement in clay stability but not in aggregate water-stability was observed in cultivated soils. The results showed a significant increase in soil organic C pool but unimproved macro-aggregation of the desert soil after 10 years of cultivation.     

Earthworm burrowing in laboratory microcosms as influenced by soil temperature and moisture

Earthworm burrows contribute to soil macroporosity and support diverse microbial communities. It is not well known how fluctuations in soil temperature and moisture affect the burrowing activities of earthworms. The objective of this experiment was to evaluate the maximum depth and length of burrows created by the endogeic earthworm Aporrectodea caliginosa (Savigny) and the anecic earthworm Lumbricus terrestris L. for a range of temperatures (5–20 °C) and soil water potentials (−5 and −11 kPa). The laboratory microcosm was a plexiglass chamber (45 cm high, 45 cm wide) containing 0.14 m² of pre-moistened soil and litter, designed to house a single earthworm for 7 days. Earthworm mass, surface casting and burrowing activities were affected significantly by soil temperature, moisture and the temperature×moisture interaction. Burrow length and maximum burrow depth increased with increasing temperature, but there was less burrowing in wetter soil (−5 kPa) than drier soil (−11 kPa). Weight gain and surface casting, however, were greater in soil at −5 kPa than −11 kPa. Our results suggest more intensive feeding and limited burrowing in wetter soil than drier soil. Earthworms inhabiting the non-compacted, drier soil may have pushed aside particles without ingesting them to create burrows. The result was that earthworms explored a larger volume of soil, deeper in the chamber, when the soil was drier. How these burrowing activities may affect the community structure and activity of soil microorganisms and microfauna in the drilosphere remains to be determined.     

Physical characterization of a soil amended with organic residues in a rice–wheat cropping system using a single value soil physical index

The effect of soil incorporations of lantana (Lantana spp.) biomass, an obnoxious weed, on physical environment of a silty clay loam soil (Typic Hapludalf) under rice (Oryza sativa L.)–wheat (Triticum aestivum L.) cropping was studied in a long-term field experiment conducted in a wet temperate region of north India. Fresh lantana biomass was incorporated into the plough layer at 10, 20 and 30 Mg ha⁻¹ annually, 7–10 days before puddling. Plant-available water capacity (PAWC), non-limiting water range (NLWR) and NLWR:PAWC ratio were determined to characterize soil physical environment during wheat crop in the tenth cropping cycle.

Ten annual applications of lantana at 10, 20 and 30 Mg ha⁻¹, increased organic carbon (OC) content over control by 12.6, 17.6 and 27.9% in 0–15 cm soil layer, and 17.1, 26.3 and 39.5% in 15–30 cm soil layer, respectively. The OC content in 0–15 and 15–30 cm soil layer of control plots was 11.1 and 7.6 g kg⁻¹ soil. Bulk density decreased by 3–14% in 7.5–10.5 cm layer and 1–6% in 15–18 cm layer. Volumetric moisture contents at 10% air-filled porosity were 38.4, 40.0, 54.5 and 55.7% at 7.5–10.5 cm depth, and 31.4, 32.2, 33.9 and 34.6% at 15–18 cm depth corresponding to 0, 10, 20 and 30 Mg ha⁻¹ lantana treatment, respectively. At 15–18 cm soil depth, volumetric moisture contents at 2 MPa soil penetration resistance were 26.9, 24.8, 23.0 and 19.6% in zero, 10, 20 and 30 Mg ha⁻¹ lantana-treated plots, respectively. Lower soil water contents associated with 10% air-filled porosity and greater soil water contents associated with a limiting penetration resistance of 2 MPa resulted in a lower NLWR (4.3%) for control as compared to lantana-treated soil (7.4–15.1%). The PAWC showed slight increase from 12.9 to 13.4–14.9% due to lantana additions. The NLWR:PAWC ratio was also lower in control (0.33) as compared to lantana-treated soil (0.55–1.01). The NLWR was significantly and positively correlated with wheat grain yield (r=0.858^**).     

Activity and biomass of soil microorganisms at different depths

We measured microbial biomass C and soil organic C in soils from one grassland and two arable sites at depths of between 0 and 90 cm. The microbial biomass C content decreased from a maximum of 1147 (0–10 cm layer) to 24 g g^-1 soil (70–90 cm layer) at the grassland site, from 178 (acidic site) and 264 g g^-1 soil (neutral site) at 10–20 cm to values of between 13 and 12 g g^-1 soil (70–90 cm layer) at the two arable sites. No significant depth gradient was observed within the plough layer (0–30 cm depth) for biomass C and soil organic C contents. In general, the microbial biomass C to soil organic C ratio decreased with depth from a maximum of between 1.4 and 2.6% to a minimum of between 0.5 and 0.7% at 70–90 cm in the three soils. Over a 24-week incubation period at 25°C, we examined the survival of microbial biomass in our three soils at depths of between 0 and 90 cm without external substrate. At the end of the incubation experiment, the contents of microbial biomass C at 0–30 cm were significantly lower than the initial values. At depths of between 30 and 90 cm, the microbial biomass C content showed no significant decline in any of the four soils and remained constant up to the end of the experiment. On average, 5.8% of soil organic C was mineralized at 0–30 cm in the three soils and 4.8% at 30–90 cm. Generally, the metabolic quotient qCO₂ values increased with depth and were especially large at 70–90 cm in depth.     

Crop yield and soil fertility response to reduced tillage under organic management

Conservation tillage (no-till and reduced tillage) brings many benefits with respect to soil fertility and energy use, but it also has drawbacks regarding the need for synthetic fertilizers and herbicides. Our objective was to adapt reduced tillage to organic farming by quantifying effects of tillage (plough versus chisel), fertilization (slurry versus manure compost) and biodynamic preparations (with versus without) on soil fertility indicators and crop yield. The experiment was initiated in 2002 on a Stagnic Eutric Cambisol (45% clay content) near Frick (Switzerland) where the average annual precipitation is 1000 mm. This report focuses on the conversion period and examines changes as tillage intensity was reduced. Soil samples were taken from the 0–10 and 10–20 cm depths and analysed for soil organic carbon (C_org), microbial biomass (C_mic), dehydrogenase activity (DHA) and earthworm density and biomass. Among the components tested, only tillage had any influence on these soil fertility indicators. C_org in the 0–10 cm soil layer increased by 7.4% (1.5 g C_org kg⁻¹ soil, p < 0.001) with reduced tillage between 2002 and 2005, but remained constant with conventional tillage. Similarly, C_mic was 28% higher and DHA 27% (p < 0.001) higher with reduced than with conventional tillage in the soil layer 0–10 cm. In the 10–20 cm layer, there were no significant differences for these soil parameters between the tillage treatments. Tillage had no significant effect on total earthworm density and biomass. The abundance of endogeic, horizontally burrowing adult earthworms was 70% higher under reduced than conventional tillage but their biomass was 53% lower with reduced tillage. Wheat (Triticum aestivum L.) and spelt (Triticum spelta L.) yield decreased by 14% (p < 0.001) and 8% (p < 0.05), respectively, with reduced tillage, but sunflower (Helianthus annuus L.) yield was slightly higher with reduced tillage. Slurry fertilization enhanced wheat yield by 5% (p < 0.001) compared to compost fertilization. Overall, C_org, C_mic, and DHA improved and yields showed only a small reduction with reduced tillage under organic management, but long-term effects such as weed competition remain unknown.     

Management of a previously eroded tropical Alfisol with herbaceous legumes: Soil loss and physical properties under mound tillage

A study was carried out on a previously eroded Oxic Paleustalf in Ibadan, southwestern Nigeria to determine the extent of soil degradation under mound tillage with some herbaceous legumes and residue management methods. A series of factorial experiments was carried out on 12 existing runoff plots. The study commenced in 1996 after a 5-year natural fallow. Mound tillage was introduced in 1997 till 1999. The legumes – Vigna unguiculata (cowpea), Mucuna pruriens and Pueraria phaseoloides – were intercropped with maize in 1996 and 1998 while yam was planted alone in 1997 and 1999. This paper covers 1997–1999. At the end of each year, residues were either burned or mulched on respective plots. Soil loss, runoff, variations in mound height, bulk density, soil water retention and sorptivity were measured. Cumulative runoff was similar among interactions of legume and residue management in 1997 (57–151 mm) and 1999 (206–397 mm). However, in 1998, cumulative runoff of 95 mm observed for Mucuna-burned residue was significantly greater than the 46 mm observed for cowpea-burned residue and the 39–51 mm observed for mulched residues of cowpea, Mucuna and Pueraria. Cumulative soil loss of 7.6 Mg ha⁻¹ observed for Mucuna-burned residue in 1997 was significantly greater than those for Pueraria-mulched (0.9 Mg ha⁻¹) and Mucuna-mulched (1.4 Mg ha⁻¹) residues whereas in 1999 it was similar to soil loss from cowpea treatments and Pueraria-burned residue (2.3–5.3 Mg ha⁻¹). There were no significant differences in soil loss in 1998 (1–3.2 Mg ha⁻¹) whereas Mucuna-burned residue had a greater soil loss (28.6 Mg ha⁻¹) than mulched cowpea (6.9 Mg ha⁻¹) and Pueraria (5.4 Mg ha⁻¹). Mound heights (23 cm average) decreased non-linearly with cumulative rainfall. A cumulative rainfall of 500 mm removed 0.3–2.3 cm of soil from mounds in 1997, 3.5–6.9 cm in 1998 and 2.3–4.6 cm in 1999, indicating that (detached but less transported) soil from mounds was far higher than observed soil loss in each year. Soil water retention was improved at potentials ranging from −1 to −1500 kPa by Mucuna-mulched residue compared to the various burned-residue treatments. Also, mound sorptivity at −1 cm water head (14.3 cm h^−1/2) was higher than furrow sorptivity (8.5 cm h^−1/2), indicating differences in hydraulic characteristics between mound and furrow. Pueraria-mulched residues for mounds had the highest sorptivity of 17.24 cm h^−1/2, whereas the least value of 6.96 cm h^−1/2 was observed in furrow of Mucuna-burned residue. Pueraria phas eoloides was considered the best option for soil conservation on the previously eroded soil, cultivated with mound tillage.     

CO2 evolution and N mineralization after biogas slurry application in the field and its yield effects on spring barley

The objective of this study was to investigate the effects of biogas slurry derived from straw-rich farmyard manure on the soil microbial biomass, on the mineralization in the field and on the related crop yield. The experiment was carried out in the following four treatments: (1) fallow, (2) fallow + biogas slurry, (3) spring barley, and (4) spring barley + biogas slurry. The CO₂ evolution rate ranged between 15 and 120 mg C m⁻² h⁻¹ in both fallow treatments and showed a significant exponential relationship with the soil temperature at 5 cm depth. According to the extrapolation of the CO₂ evolution rates into amounts per hectare, approximately 200 kg C ha⁻¹ or 27% of the biogas slurry derived C were mineralized to CO₂ during a 50 days’ period to 18 June in the fallow treatment with biogas slurry. An additional amount of up to 29.5 kg inorganic N ha⁻¹ could be calculated as the sum of NH₄-N already present in biogas slurry at the time of amendment and from the amount of biogas slurry mineralized in the soil to NO₃-N. A good agreement between measured and modelled stocks of inorganic N at 0–60 cm depth was obtained after having five-fold increased soil organic C turnover compared to the default values of the model DNDC. The mineralization data are in line with an amount of up to 21 kg ha⁻¹ more N transferred by the barley plants to their aboveground biomass in biogas slurry treatment. The N not accounted for by the aboveground plant biomass could be explained by the belowground plant-derived N. CO₂ evolution from the soil surface, inorganic N content at 0–60 cm depth and N transfer into barley aboveground biomass lead apparently to similar results after the application of biogas slurry. The soil ATP content after harvest of the barley was significantly larger in the two treatments with biogas slurry, especially in the fallow treatment indicating a positive effect on the soil microbial community.     

Soil chemical and microbiological properties along a chronosequence of Caragana microphylla Lam. plantations in the Horqin sandy land of Northeast China

Caragana microphylla Lam., a leguminous shrub, is a dominant native plant species widely planted to stabilize the moving and semi-moving sand dunes in the semi-arid Horqin sandy land of Northeast China. The objective of this study was to determine how C. microphylla plantations affected the physical, chemical and microbiological properties of a sandy soil. Soil samples at the depths of 0–10, 10–20, 20–30, 30–40, and 40–50 cm were collected from the C. microphylla plantations with an age sequence of 0, 5, 10, and 23 years. The results showed that shrub growth altered microclimate, increased litter input, and hence, improved soil water holding capacity, contents of total carbon, total N and microbial biomass C and N, electrical conductivity, and activities of urease, phosphomonoesterase, protease, dehydrogenase and polyphenol oxidase, and decreased soil bulk density. These trends increased with increasing plantation age but decreased with increasing soil depth. C. microphylla establishment could be an effective and applicable measure to restore vegetation and control desertification in the Horqin sandy land, and recommended for adoption in semi-arid sandy areas on a large scale.     

Influence of warming and enchytraeid activities on soil CO2 and CH4 fluxes

To determine the sum of ‘direct’ and ‘indirect’ effects of climatic change on enchytraeid activity and C fluxes from an organic soil we assessed the influence of temperature (4, 10 and 15 °C incubations) on enchytraeid populations and soil CO₂ and CH₄ fluxes over 116 days. Moisture was maintained at 60% of soil dry weight during the experimental period and measurements of enchytraeid biomass and numbers, and CO₂ and CH₄ fluxes were made after 3, 16, 33, 44, 65, 86 and 116 days. Enchytraeid population numbers and biomass increased in all temperature treatments with the greatest increase produced at 15 °C (to over threefold initial values by day 86). Results also showed that enchytraeid activity increased CO₂ fluxes by 10.7±4.5, 3.4±4.0 and 26.8±2.6% in 4, 10 and 15 °C treatments, respectively, with the greatest CO₂ production observed at 15 °C for the entire 116 day incubation period (P<0.05). The soil respiratory quotient analyses at lower temperatures (i.e. 4-10 °C) gave a Q₁₀ of 1.7 and 1.9 with and without enchytraeids, respectively. At temperatures above 10 °C (i.e. 10-15 °C) Q₁₀ significantly increased (P<0.01) and was 25% greater in the presence of enchytraeids (Q₁₀=3.4) than without (Q₁₀=2.6). In contrast to CO₂ production, no significant relationships were observed between net CH₄ fluxes and temperature and only time showed a significant effect on CH₄ production (P<0.01).Total soil CO₂ production was positively linked with enchytraeid biomass and mean soil CO₂-C production was 77.01±6.05 CO₂-C μg mg enchytraeid tissue⁻¹ day⁻¹ irrespective of temperature treatment. This positive relationship was used to build a two step regression model to estimate the effects of temperature on enchytraeid biomass and soil CO₂ respiration in the field. Predictions of potential CO₂ production were made using enchytraeid biomass data obtained in the field from two upland grassland sites (Sourhope and Great Dun Fell at the Moor House Nature Reserve, both in the UK). The findings of this work suggest that a 5 °C increase in atmospheric temperature above mean ambient temperature could have the potential to produce a significant increase in enchytraeid biomass resulting in a near twofold increase in soil CO₂ release from both soil types. The interaction between temperature and soil biology will clearly be an important determinant of soil respiration responses to global warming.     

Spatial patterns of soil biological and physical properties in a ridge tilled and a ploughed Luvisol

The present study was conducted to determine the spatial heterogeneity of bulk density, soil moisture, inorganic N, microbial biomass C, and microbial biomass N in the ridge tillage system of Turiel compared to conventional mouldboard ploughing on three sampling dates in May, July, and August. The soil sampling was carried out under vegetation representing the ridge in a high spatial resolution down the soil profile. Bulk density increased with depth and ranged from 1.3 g cm⁻³ at 10 cm depth to 1.6 g cm⁻³ at 35 cm in ploughed plots and from 1.0 g m⁻³ at 5 cm to 1.4 g m⁻³ at 35 cm in the ridges. In the ploughed plots, the contents of microbial biomass C and microbial biomass N remained roughly constant at 215 and 33 μg g⁻¹ soil, respectively, throughout the experimental period. The microbial biomass C/N ratio varied in a small range around 6.4. In the ridged plots, the contents of microbial biomass C and microbial biomass N were 5% and 6% higher compared to the ploughed plots. Highest microbial biomass C contents of roughly 300 μg g⁻¹ soil were always measured in the crowns in July. The lowest contents of microbial biomass C of 85–137 μg g⁻¹ soil were measured in the furrows. The ridges showed strong spatial heterogeneity in bulk density, soil water content, inorganic nitrogen and microbial biomass.     

Effect of tractor weight, depth of ploughing and wheel placement during ploughing in an organic cereal rotation on contrasting soils

The relative effects of using light (2–3 Mg) versus heavier (5–7 Mg) tractors, shallow (15 cm) versus deeper (25 cm) ploughing and on-land versus in-furrow wheel placement during ploughing were investigated from 2003 to 2006 in organic rotations (wheat or barley, green manure, oats with peas) and conventionally fertilized barley. Trials were located on loam soil in south-eastern Norway and silty clay loam in central Norway. Ploughing was performed in spring, when the topsoil moisture content was at or below field capacity, using single furrow ploughs that allowed alternative wheel placement and resulted in complete coverage of the surface by wheels each year (ca. 3 times the normal coverage during ploughing). Low tyre inflation pressures (≤80 kPa) were used throughout. The use of a heavy tractor increased topsoil bulk density slightly in the loam soil, and, in combination with in-furrow wheeling, it reduced air-filled pore space and air permeability at 18–22 cm. On the silty clay loam, the use of a heavy tractor did not increase bulk density, but it reduced air-filled pore space throughout the topsoil. In-furrow wheeling reduced air-filled pore space in this soil also, compared to on-land wheeling. Penetration resistance was in this soil always greater at 15–25 cm depth after shallow than after deep ploughing, especially with in-furrow rather than on-land wheeling. Shallow ploughing led on both soils to marked increases in perennial weed biomass compared to deep ploughing. Earthworms were hardly affected by the treatments, but in the loam in 2006 a higher number of individuals were found where the light rather than the heavy tractor had been used. Few significant treatment effects were found on grain yield and quality. Deep ploughing with a light tractor gave the highest wheat yield and protein content in 2 years on the loam soil, and on the silty clay loam the yield of conventionally fertilized barley was higher after deep than after shallow ploughing. In summary, limited evidence was found to support the use of on-land rather than in-furrow wheeling when ploughing is performed at favourable soil moisture and with tractor weights < 5 Mg. There is, however, reason to be wary of using heavy tractors (>5 Mg), even under such conditions. With regard to ploughing depth in organic rotations dominated by cereals, the need to combat perennial weeds by deep ploughing weighs probably more heavily than any possible beneficial effect of shallow ploughing on stimulating nutrient turnover.     

The effect of tillage systems on some microbial characteristics

Soils were sampled from plots with four variants of tillage methods: (1) conventional tillage (CT); (2) no tillage (NT); (3) minimum tillage (MTS); and (4) no tillage + mulch (NTM). Our aim was to study the influence of tillage on selected soil microbial properties. Determination of urease, dehydrogenase, invertase, arylsulphatase, potential nitrogenase activity, CFU of Azotobacter spp., and carbon microbial biomass has been conducted for time period 2002–2007. Soil samples from 0–0.1 m, 0.1–0.3 m, and 0.3–0.5 m were collected in the spring and autumn. Enzymatic activities (dehydrogenase, urease, arylsulphatase, and invertase) were significantly affected by soil depth and the tillage system employed. The statistically significant higher activity of urease was measured using the minimum tillage system (MTS), compared to the conventional tillage (CT) at soil depths of 0–0.1 m. The highest dehydrogenase activity was measured during the protective tillage treatment (NTM – no tillage with mulch). As far as other enzymatic activities (invertase and arylsulphatase) are concerned, the highest values were recorded in the protective tillage treatments. The highest counts of Azotobacter spp., as well as the highest nitrogenase activity (both statistically significant) were found in the no tillage + mulch (NTM) variant, at depths of 0.1–0.3 m. Microbial biomass (C-biomass) was the highest with the minimum tillage (MTS). The results show a positive influence of protection soil tillage on the reviving of upper layer of topsoil, especially in the variants where soil was supplied with organic matter.     

Microbiological parameters as indicators of soil quality under various soil management and crop rotation systems in southern Brazil

The objective of this work was to identify soil parameters potentially useful to monitor soil quality under different soil management and crop rotation systems. Microbiological and chemical parameters were evaluated in a field experiment in the State of Paraná, southern Brazil, in response to soil management [no-tillage (NT) and conventional tillage (CT)] and crop rotation [including grain (soybean, S; maize, M; wheat, W) and legume (lupin, L.) and non-legume (oat, O) covers] systems. Three crop rotation systems were evaluated: (1) (O/M/O/S/W/S/L/M/O/S), (2) (O/S/L/M/O/S/W/S/L/M), and (3) (O/S/W/S/L/M/O/M/W/M), and soil parameters were monitored after the fifth year. Before ploughing, CO₂-emission rates were similar in NT and CT soils, but plough increased it by an average of 57%. Carbon dioxide emission was 13% higher with lupin residues than with wheat straw; decomposition rates were rapid with both soil management systems. Amounts of microbial biomass carbon and nitrogen (MB-C and MB-N, respectively) were 80 and 104% higher in NT than in CT, respectively; however, in general these parameters were not affected by crop rotation. Efficiency of the microbial community was significantly higher in NT: metabolic quotient (qCO₂) was 55% lower than in CT. Soluble C and N levels were 37 and 24% greater in NT than in CT, respectively, with no effects of crop rotation. Furthermore, ratios of soluble C and N contents to MB-C and MB-N were consistently lower in NT, indicating higher immobilization of C and N per unit of MB. The decrease in qCO₂ and the increase in MB-C under NT allowed enhancements in soil C stocks, such that in the 0–40 cm profile, a gain of 2500 kg of C ha⁻¹ was observed in relation to CT. Carbon stocks also varied with crop rotation, with net changes at 0–40 cm of 726, 1167 and −394 kg C ha⁻¹ year, in rotations 1, 2 and 3, respectively. Similar results were obtained for the N stocks, with 410 kg N ha⁻¹ gained in NT, while crop rotations 1, 2 and 3 accumulated 71, 137 and 37 kg of N ha⁻¹ year⁻¹, respectively. On average, microbial biomass corresponded to 2.4 and 1.7% of the total soil C, and 5.2 and 3.2% of the N in NT and CT systems, respectively. Soil management was the main factor affecting soil C and N levels, but enhancement also resulted from the ratios of legumes and non-legumes in the rotations. The results emphasize the importance of microorganisms as reservoirs of C and N in tropical soils. Furthermore, the parameters associated with microbiological activity were more responsive to soil management and crop rotation effects than were total stocks of C and N, demonstrating their usefulness as indicators of soil quality in the tropics.