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1.
This study aims to determine leaf litter preference, consumption rate, growth rate, food conversion efficiency, and quality
of fecal pellets of two endemic pill millipedes (Arthrosphaera dalyi and Arthrosphaera davisoni) of the Western Ghats of India by laboratory microcosm experiments. Among seven combinations of three plantation leaf litters
offered in 4-day trial, top three preferred combinations were selected for 4-week trial. In 4-week trial, preference of mixed
litter diet was higher than single litter diet, which resulted in enhanced growth as well as food conversion efficiency of
millipedes. Among Hopea
,
Pongamia
, and Areca litters, A. dalyi preferred Hopea + Pongamia, and its consumption was significantly correlated with contents of organic carbon (P < 0.05; r = –0.97) and nitrogen (P < 0.01; r = 0.99), while growth rate with phosphorus content (P < 0.05; r = 0.97) and food conversion efficiency with contents of organic carbon (P < 0.05; r = 0.98) and calcium (P < 0.01; r = –0.99). Among Areca
,
Elettaria
, and Coffea litters, Areca + Elettaria
+ Coffea was most preferred by A. davisoni, which was significantly correlated with organic carbon content (P < 0.05; r = 0.98) and food conversion efficiency with calcium content (P < 0.0001; r = 0.99). The food conversion efficiency, however, was the highest in millipedes fed with Areca
+
Elettaria. The present study demonstrated increased nitrogen and phosphorus contents and decreased phenolic content and C/N ratio in
fecal pellets of pill millipedes fed with plantation litter, and thus, these millipedes play an important role in leaf litter
mineralization and soil enrichment in plantations Western Ghats. 相似文献
2.
A study was conducted to investigate the effects of cow manure and sewage sludge application on the activity and kinetics
of soil l-glutaminase. Soil samples were collected from a farm experiment in which 0, 25, and 100 Mg ha−1 of either cow manure or sewage sludge had been applied annually for 4 consecutive years to a clay loam soil (Typic Haplargid).
A chemical fertilizer treatment had also been applied. Results indicated that the effects of chemical fertilizer and the solid
waste application on pH in the 18 surface soil (0–15 cm) samples were not significant. The organic C content, however, was
affected significantly by the different treatments, being the greatest in soils treated with 100 Mg ha−1 cow manure, and the least in the control treatment. l-Glutaminase activity was generally greater in solid-waste applied soils and was significantly correlated (r = 0.939, P < 0.001) with organic C content of soils. The values of l-glutaminase maximum velocity (Vmax) ranged from 331 to 1,389 mg NH4
+–N kg−1 2 h−1. Values of the Michaelis constant (K
m) ranged from 35.1 to 71.7 mM. Organic C content of the soils were significantly correlated with V
max (r = 0.919, P < 0.001) and K
m (r = 0.763, P < 0.001) values. These results demonstrate the considerable influence that solid waste application has on this enzymatic
reaction involved in N mineralization in soil. 相似文献
3.
A 15N dilution experiment was carried out to investigate effects of cultivation on the gross N transformation rate in coastal
wetland zone. Microbial community composition was estimated by phospholipid fatty acid (PLFA) analysis and abundance of soil
ammonia-oxidizing bacteria (AOB) was quantified by real-time polymerase chain reaction (PCR). Soil salinity decreased significantly,
while total N increased after coastal wetland was cultivated. Microbial biomass (total PLFA), bacterial biomass, fungal biomass,
and actinomycete biomass of the native coastal wetland soils were significantly (p < 0.05) lower than those of the cultivated soils whereas AOB population size also significantly increased after coastal wetland
cultivation. Multiple regression analysis showed that total PLFA biomass and soil total N (TN) explained 97% of the variation
of gross N mineralization rate in the studied soils (gross mineralization rate = 0.179 total PLFA biomass + 5.828TN − 2.505,
n = 16, p < 0.01). Gross nitrification rate increased by increasing the soil AOB population size and gross mineralization rate (M) (gross nitrification rate = 3.39AOB + 0.18 M − 0.075, R
2 = 0.98, n = 16, p < 0.01). Management of salt discharge and mineral N fertilization during the cultivation of wetland soils might have changed
composition of soil microflora and AOB population size, thus influencing mineralization and nitrification. Probably, the cultivation
of coastal wetland soils increased the risk of N losses from soil through nitrate leaching and gas emission (e.g., N2O and NO). 相似文献
4.
The long-term treatment effect (since 1957–1966) of farmyard manure (FYM) application compared with crop residue incorporation
was investigated in five soils (sandy loam to silty clay) with regards to the net sulfur (S) mineralization potential. An
open incubation technique was used to determine accumulated net S mineralization (SAccMin) and a number of soil physical and chemical properties were determined. Treatments and soil differences in SAccMin, as well as correlations with soil variables, were tested with single and multivariate analyses. Long-term FYM application
resulted in a significantly (p = 0.012) higher net S mineralization potential, although total amounts of C, N, and S were not significantly (p < 0.05) increased. The accumulated S mineralization differed significantly (p < 0.05) between soils within this treatment. The measured soil variables were not significantly correlated to SAccMin. Conclusively, different treatment histories influenced the quality (e.g., chemical composition) and cycling rate of the
organic S pool, rather than its size. 相似文献
5.
Shixue Yin Yuanhua Dong Yangchun Xu Qiwei Huang Qirong Shen 《Biology and Fertility of Soils》2011,47(3):303-313
Rice seedling wilt frequently occurs in upland nurseries under well-aerated conditions and causes considerable economic loss.
Whether the wilt is pathogenic or edaphic is not known. We hypothesize the use of composts to alleviate seedling wilt. The
severity level of upland rice seedling wilt was significantly (p < 0.05) positively correlated with soil pH (r = 0.499; n = 19), but negatively correlated with soil organic matter (r = −0.745), microbial biomass C (r = −0.669), activities of dehydrogenase (r = −0.589), arylsulfatase (r = −0.272), fluorescein diacetate hydrolysis (r = −0.466), and β-glucosidase (r = −0.280). Correlations between severity level and soil inorganic N and exchangeable potassium K were not significant. Contents
of Fe, Zn, Cu, and Mn in healthy seedlings were not significantly (p < 0.05) different from those in infected seedlings. These data suggest that seedling wilts are not associated with nutrient
constraints. Compost amendment at the rate of 3% or above in pot experiments significantly improved seedling growth and reduced
the wilt symptoms. Field trials further showed that aboveground weight of seedlings in compost-amended treatment ranged from
11.5 to 14.9 mg per plant, significantly higher than the range from 6.38 to 12.1 mg per plant in the control treatment; in
addition to rice growth compost significantly increased microbial biomass and enzyme activities of soils. Soil fumigation
significantly increased rice growth and alleviation symptoms in 11 out of 19 soils, suggesting the involvement of pathogens.
It is concluded that upland seedling wilt is a pathogen-associated disease. Probably high soil pH and low soil biochemical
activities may favor pathogen activities. 相似文献
6.
Pamela H. Templer Andrew F. Schiller Nathan W. Fuller Anne M. Socci John L. Campbell John E. Drake Thomas H. Kunz 《Biology and Fertility of Soils》2012,48(4):413-424
Projected changes in climate for the northeastern USA over the next 100 years include a reduction in the depth and duration
of the winter snowpack, which could affect soil temperatures and frost regimes. We conducted a snow-removal experiment in
a northern hardwood forest at the Hubbard Brook Experimental Forest in central New Hampshire over 2 years to induce soil freezing
and evaluate its effect on the abundance, richness, and diversity of soil arthropods during the growing season. Snow removal
at the beginning of winter increased the depth and duration of soil frost, decreased soil temperatures, and led to a reduced
abundance of some arthropod taxa, including Araneae (reduced by 57%; P = 0.0001), Pseudoscorpionida (75%; P < 0.0001), Hymenoptera (57%; P = 0.0033), Collembola (24%; P = 0.0019), adult Coleoptera (23%; P = 0.0057), and larval Diptera (33%; P < 0.0001) and an increase in other taxa, including Hemiptera (increased by 7%; P = 0.032). Taxa that did not respond significantly to snow removal included Chilopoda (P = 0.55), Acari (P = 0.66), Diplopoda (P = 0.66), adult Diptera (P = 0.54), and larval Coleoptera (P = 0.39). A delayed snowpack over two winters also resulted in decreased arthropod richness by 30% (P < 0.0001) and Simpson’s index of diversity by 22% (P = 0.0002) during the two subsequent growing seasons. Results of this study demonstrate that predicted changes in the winter
snowpack and depth and duration of soil frost may reduce the abundance and alter the community composition of arthropods living
in the forest floor of northern hardwood forests, which could have implications for the structure and function of northern
forest ecosystems. 相似文献
7.
Balaji Anandha Rao Cameron P. Wake Todd Anderson William Andrew Jackson 《Water, air, and soil pollution》2012,223(1):181-188
Temporal depositional rates are important in order to understand the production and occurrence of perchlorate (ClO4−) as limited information exists regarding the impact of anthropogenic production or atmospheric pollution on ClO4− deposition. Perchlorate concentrations in discrete ice core samples from the Eclipse Icefield (Yukon Territory, Canada) and
Upper Fremont Glacier (Wyoming, USA) were analyzed using ion chromatography tandem mass spectrometry to evaluate temporal
changes in the deposition of ClO4
− in North America. The ice core samples cover a time period from 1726 to 1993 and 1970 to 2002 for the Upper Fremont Glacier
(UFG) and Eclipse ice cores, respectively. The average ClO4
− concentration in the Eclipse ice core for the time period from 1970 to 1973 was 0.6 ± 0.3 ng L−1, with higher values of 2.3 ± 1.7 and 2.2 ± 2.0 ng L−1 for the periods 1982–1986 and 1999–2002, respectively. All pre-1980 ice core samples from the UFG had ClO4
− concentrations <0.2 ng L−1, and the post-1980 samples ranged from <0.2 ng L−1 to a maximum of 2.6 ng L−1 for the year 1992. A significant positive correlation (R = 0.75, N = 15, p < 0.001) of ClO4− with SO42− was found for the annual UFG ice core layers and of ClO4
− with SO42− and NO3− in sub-annual Eclipse ice samples (R > 0.3, N = 121, p < 0.002). The estimated yearly ClO4− depositional flux for the Eclipse ice core ranged from 0.6 (1970) to 4.7 μg m−2 year−1 (1982) and the UFG from <0.1 (pre-1980) to 1.4 μg m−2 year−1 (1992). There was no consistent seasonal variation in the ClO4− depositional flux for the Eclipse ice core, in contrast to a previous study on the Arctic region. The presence of ClO4− in these ice cores might correspond to an intermittent source such as volcanic eruptions and/or any anthropogenic forcing
that may directly or indirectly aid in atmospheric ClO4− formation. 相似文献
8.
Crop residues and fertilizer nitrogen influence residue decomposition and nitrous oxide emission from a Vertisol 总被引:2,自引:0,他引:2
Wisal Muhammad Sarah M. Vaughan Ram C. Dalal Neal W. Menzies 《Biology and Fertility of Soils》2011,47(1):15-23
Crop residues with high C/N ratio immobilize N released during decomposition in soil, thus reducing N losses through leaching,
denitrification, and nitrous oxide (N2O) emission. A laboratory incubation experiment was conducted for 84 days under controlled conditions (24°C and moisture content
55% of water-holding capacity) to study the influence of sugarcane, maize, sorghum, cotton and lucerne residues, and mineral
N addition, on N mineralization–immobilization and N2O emission. Residues were added at the rate of 3 t C ha−1 to soil with, and without, 150 kg urea N ha−1. The addition of sugarcane, maize, and sorghum residues without N fertilizer resulted in a significant immobilization of
soil N. Amended soil had significantly (P < 0.05) lower NO3−–N, which reached minimum values of 2.8 mg N kg−1 for sugarcane (at day 28), 10.3 mg N kg−1 for maize (day 7), and 5.9 mg N kg−1 for sorghum (day 7), compared to 22.7 mg N kg−1 for the unamended soil (day 7). During 84 days of incubation, the total mineral N in the residues + N treatments were decreased
by 45 mg N kg−1 in sugarcane, 34 mg kg−1 in maize, 29 mg kg−1 in sorghum, and 16 mg kg−1 in cotton amended soil compared to soil + N fertilizer, although soil NO3−–N increased by 7 mg kg−1 in lucerne amended soil. The addition of residues also significantly increased amended soil microbial biomass C and N. Maximum
emissions of N2O from crop residue amended soils occurred in the first 4–5 days of incubation. Overall, after 84 days of incubation, the
cumulative N2O emission was 25% lower with cotton + N fertilizer, compared to soil + N fertilizer. The cumulative N2O emission was significantly and positively correlated with NO3−–N (r = 0.92, P < 0.01) and total mineral N (r = 0.93, P < 0.01) after 84 days of incubation, and had a weak but significant positive correlation with cumulative CO2 in the first 3 and 5 days of incubation (r = 0.59, P < 0.05). 相似文献
9.
Nitrogen and carbon mineralization of cattle manure (N=6 g kg–1; C:N=35), pressmud (N=17.4 g kg–1; C:N=22), green manure (N=26.8 g kg–1; C:N=14) and poultry manure (N=19.5 g kg–1; C:N=12) and their influence on gaseous N losses via denitrification (using the acetylene inhibition technique) in a semiarid subtropical soil (Typic Ustochrepts) were investigated in a growth chamber simulating upland, nearly saturated, and flooded conditions. Mineralization of N started quickly in all manures, except pressmud where immobilization of soil mineral N was observed for an initial 4 days. Accumulation of mineral N in upland soil plus denitrified N revealed that mineralization of cattle manure-, pressmud-, poultry manure- and green manure-N over 16 days was 12, 20, 29 and 44%, respectively, and was inversely related to C:N ratio (R 2=0.703, P=0.05) and directly to N content of organic manure (R 2=0.964, P=0.01). Manure-C mineralized over 16 days ranged from 6% to 50% in different manures added to soil under different moisture regimes and was, in general, inversely related to initial C:N ratio of manure (R 2=0.690, P=0.05). Cumulative denitrification losses over 16 days in control soils (without manure) under upland, nearly saturated, and flooded conditions were 5, 23, and 24 mg N kg–1, respectively. Incorporation of manures enhanced denitrification losses by 60-82% in upland, 52–163% in nearly saturated, and 26–107% in flooded soil conditions over a 16-day period, demonstrating that mineralized N and C from added manures could result in 2- to 3-fold higher rate of denitrification. Cumulative denitrification losses were maximal with green manure, followed by poultry manure, pressmud and cattle manure showing an increase in denitrification with increasing N content and decreasing C:N ratio of manure. Manure-amended nearly saturated soils supported 14–35% greater denitrification than flooded soils due to greater mineralization and supply of C. 相似文献
10.
Conservation tillage, and especially no-tillage, induce changes in the distribution of organic pools in the soil profile.
In long-term field experiments, marked stratification of the total soil microbial biomass and its activity have been observed
as consequence of the application of no-tillage to previously tilled soils. Our objective was to study the evolution of the
total and active soil microbial biomass and mineralized C in vitro during the first crop after the introduction of no-tillage
to an agricultural soil. The experiment was performed on a Typic Hapludoll from the Argentinean Pampa. Remaining plant residues,
total and active microbial biomass and mineralized C were determined at 0–5 cm and 5–15 cm depths, at three sampling times:
wheat tilling, silking and maturity. The introduction of no-tillage produced an accumulation of plant residues in the soil
surface layer (0–5 cm), showing stratification with depth at all sampling dates. Active microbial biomass and C mineralization
were higher under no-tillage than under conventional tillage in the top 5 cm of the profile. The total soil microbial biomass
did not differ between treatments. The active soil biomass was highly and positive correlated with plant residues (r
2=0.617;P<0.01) and with mineralized C (r
2=0.732;P<0.01). Consequently, the active microbial biomass and mineralized C reflected immediately the changes in residue management,
whereas the total microbial biomass seemed not to be an early indicator of the introduction of a new form of soil management
in our experiment.
Received: 23 February 1999 相似文献
11.
Fabrice Monna Lhoussaine Bouchaou Claire Rambeau Rémi Losno Olivier Bruguier Gaetano Dongarrà Stuart Black Carmela Chateau 《Water, air, and soil pollution》2012,223(3):1263-1274
More than 30 epiphytic lichens, collected in Agadir (Morroco) and along a 150-km transect from the Atlantic Ocean eastward,
were analyzed for their metal content and lead isotopic composition. This dataset was used to evaluate atmospheric metal contamination
and the impact of the city on the surrounding area. The concentrations of Cu, Pb, and Zn (average ± 1 SD) were 20.9 ± 15.2 μg g−1, 13.8 ± 9.0 μg g−1, and 56.6 ± 26.6 μg g−1, respectively, with the highest values observed in lichens collected within the urban area. The 206Pb/207Pb and 208Pb/207Pb ratios in the lichens varied from 1.146 to 1.186 and from 2.423 to 2.460, respectively. Alkyllead-gasoline sold in Morocco
by the major petrol companies gave isotopic ratios of 206Pb/207Pb = 1.076–1.081 and 208Pb/207Pb = 2.348–2.360. These new, homogeneous values for gasoline-derived lead improve and update the scarce isotopic database
of potential lead sources in Morocco, and may be of great value to future environmental surveys on the presence of lead in
natural reservoirs, where it persists over time (e.g., soils and sediments). The interest of normalizing metal concentrations
in lichens to concentrations of a lithogenic element is demonstrated by the consistency of the results thus obtained with
lead isotopic ratios. Leaded gasoline contributed less than 50% of the total amount of lead accumulated in lichens, even in
areas subject to high vehicular traffic. This strongly suggests that the recent banishment of leaded gasoline in Morocco will
not trigger a drastic improvement in air quality, at least in Agadir. 相似文献
12.
Long-term winter cover cropping effects on corn (Zea mays L.) production and soil nitrogen availability 总被引:1,自引:0,他引:1
This study was conducted to determine effects of long-term winter cover cropping with hairy vetch, cereal rye and annual
ryegrass on soil N availability and corn productivity. From 1987 to 1995, with the exception of the first year of the study,
the cover crops were seeded each year in late September or early October after the corn harvest and incorporated into the
soil in late April or early May. Corn was seeded 10 days to 2 weeks after the cover crop residues had been incorporated, and
N fertilizer was applied as a side-dressing at rates of 0, 67, 134, or 201 kg N ha–1 each year. While the average annual total N input from the above-ground biomass of the cover crops was highest for hairy
vetch (72.4 kg N ha–1), the average annual total C input was highest for cereal rye (1043 kg C ha–1) compared with the other cover crops. Hairy vetch was the only cover crop that significantly increased pre-side-dressed NO3
–-N (Ni) corn biomass and N uptake at 0 N. At an N fertilizer rate of 134 kg N ha–1 or higher, the cover crops had a minimal effect on corn biomass. This indicated that even after 9 years of winter cover cropping,
the effect of the cover crops on corn growth resulted primarily from their influence on soil N availability. The amount of
available N estimated from the cover crops (Nac) was significantly correlated with relative corn biomass production (r
2=0.707, P<0.001). The total amount of available N, comprising Nac and N added from fertilizer (Nf), was strongly correlated (r
2=0.820, P<0.001)) with relative corn biomass production. The correlation was also high for the available N comprising Ni and Nf (r
2=0.775, P<0.001). Although cereal rye and annual ryegrass did not improve corn biomass production in the short term, they benefited
soil organic N accumulation and gradually improved corn biomass production compared with the control over the long term.
Received: 10 August 1999 相似文献
13.
Ignacio A. Ciampitti Esteban A. Ciarlo Marta E. Conti 《Biology and Fertility of Soils》2008,44(4):581-588
The purpose of this study was to evaluate, during the phenological stages of inoculated soybean crop [Glycine max (L.) Merrill], the effect of different N fertilization levels and inoculation with Bradyrhizobium japonicum on N2O emissions from the soil. Gas emissions were evaluated at field conditions by the static-chamber method. Nitrogen fertilization
increased N2O emissions significantly (P < 0.05). The variable that best explained cumulative N2O emissions during the whole soybean growing season was the soil nitrate level (r
2 = 0.1899; P = 0.0231). Soil moisture presented a greater control on N2O emissions between the grain-filling period and the crop commercial maturity (r
2 = 0.5361; P < 0.0001), which coincided with a positive balance of the available soil N, as a consequence of the decrease in crop requirements
and root and nodular decomposition. Only soil soluble carbon (r
2 = 0.29; P = 0.019) and moisture (r
2 = 0.24; P = 0.039) were correlated with N2O emissions during the residue decomposition period. The relationship between soil variables and N2O emissions depended on crop phenological or stubbles decomposition stages. 相似文献
14.
We hypothesized that the integration of trees and shrubs in agricultural landscapes can reduce NO3
– leaching and increase utilization of subsoil N. A field survey was conducted on 14 farms on acid soils in the subhumid highlands
of Kenya, where there is little use of fertilizers, to determine the effect of vegetation types (VT) on soil NH4
+ and NO3
– to 4 m depth. The VT included maize (Zea mays) with poor growth and good growth, Markhamia lutea trees scattered in maize, natural weed fallow, banana (Musa spp.), hedgerow, and eucalyptus woodlot. The effect of VT on NH4
+ was small (<1 mg N kg–1). NO3
– within a VT was about constant with depth below 0.25 m, but subsoil NO3
– varied greatly among VT. Mean NO3
–-N concentrations at 0.5–4 m depth were low beneath hedgerow and woodlot (<0.2 mg kg–1), intermediate beneath weed fallow (0.2–0.7 mg kg–1), banana (0.5–1.0 mg kg–1) and markhamia (0.5–1.6 mg kg–1), and high beneath both poor (1.0–2.1 mg kg–1) and good (1.9–3.1 mg kg–1) maize. Subsoil NO3
– (0.5–4 m) was agronomically significant after maize harvest with 37 kg N ha–1 m–1 depth of subsoil beneath good maize and 27 kg N ha–1 m–1 depth beneath poor maize. In contrast, subsoil NO3
– was only 2 kg N ha–1 m–1 depth beneath woodlot and hedgerow. These results demonstrate that the integration of perennial vegetation and the rotation
of annual and perennial crops can tighten N cycling in agricultural landscapes.
Received: 8 July 1999 相似文献
15.
Arylsulfatase activity of microbial biomass in soils as affected by cropping systems 总被引:5,自引:0,他引:5
The impacts of crop rotations and N fertilization on different pools of arylsulfatase activity (total, intracellular, and
extracellular) were studied in soils of two long-term field experiments in Iowa to assess the contibution of the microbial
biomass to the activity of this enzyme. Surface-soil samples were taken in 1996 and 1997 in corn, soybeans, oats, or meadow
(alfalfa) plots that received 0 or 180 kg N ha–1 before corn, and an annual application of 20 kg P ha–1 and 56 kg K ha–1. The arylsulfatase activity in the soils was assayed at optimal pH (acetate buffer, pH 5.8) before and after chloroform fumigation;
microbial biomass C (Cmic) and N (Nmic) were determined by chloroform-fumigation methods. All pools of arylsulfatase activity in soils were significantly affected
by crop rotation and plant cover at sampling time, but not by N fertilization. Generally, the highest total, intracellular,
and extracellular arylsulfatase activities were obtained in soils under cereal-meadow rotations, taken under oats or meadow,
and the lowest under continuous cropping systems.Total, intracellular, and extracellular arylsulfatase activities were significantly
correlated with Cmic (r>0.41, P<0.01) and Nmic (r>0.38, P<0.01) in soils. The averages of specific activity values, i.e., of arylsulfatase activity of the microbial biomass, expressed
per milligram Cmic, ranged from 315 to 407 μg p-nitrophenol h–1. The total arylsulfatase activity was significantly correlated with the intracellular activity, with r values >0.79 (P<0.001). In general, about 45% of the total arylsulfatase activity was extracellular, and 55% was associated with the microbial
biomass in soils, indicating the importance of the microflora as an enzyme source in soils.
Received: 23 April 1998 相似文献
16.
Simple and rapid chemical indices of soil nitrogen (N)-supplying capacity are necessary for fertilizer recommendations. In
this study, pot experiment involving rice, anaerobic incubation, and chemical analysis were conducted for paddy soils collected
from nine locations in the Taihu Lake region of China. The paddy soils showed large variability in N-supplying capacity as
indicated by the total N uptake (TNU) by rice plants in a pot experiment, which ranged from 639.7 to 1,046.2 mg N pot−1 at maturity stage, representing 5.8% of the total soil N on average. Anaerobic incubation for 3, 14, 28, and 112 days all
resulted in a significant (P < 0.01) correlation between cumulative mineral NH4+-N and TNU, but generally better correlations were obtained with increasing incubation time. Soil organic C, total soil N,
microbial C, and ultraviolet absorbance of NaHCO3 extract at 205 and 260 nm revealed no clear relationship with TNU or cumulative mineral NH4+-N. Soil C/N ratio, acid KMnO4-NH4+-N, alkaline KMnO4-NH4+-N, phosphate–borate buffer extractable NH4+-N (PB-NH4+-N), phosphate–borate buffer hydrolyzable NH4+-N (PBHYDR-NH4+-N) and hot KCl extractable NH4+-N (HKCl−NH4+-N) were all significantly (P < 0.05) related to TNU and cumulative mineral NH4+-N of long-term incubation (>28 days). However, the best chemical index of soil N-supplying capacity was the soil C/N ratio,
which showed the highest correlation with TNU at maturity stage (R = −0.929, P < 0.001) and cumulative mineral NH4+-N (R = −0.971, P < 0.001). Acid KMnO4-NH4+-N plus native soil NH4+-N produced similar, but slightly worse predictions of soil N-supplying capacity than the soil C/N ratio. 相似文献
17.
Spatial changes of soil fungal and bacterial biomass from a sub-alpine coniferous forest to grassland in a humid, sub-tropical region 总被引:7,自引:0,他引:7
Fungal and bacterial biomass were determined across a gradient from a forest to grassland in a sub-alpine region in central
Taiwan. The respiration-inhibition and ergosterol methods for the evaluation of the microbial biomass were compared. Soil
fungal and bacterial biomass both significantly decreased (P<0.05) with the shift of vegetation from forest to grassland. Fungal and bacterial respiration rates (evolved CO2) were, respectively, 89.1 μl CO2 g–1 soil h–1 and 55.1 μl CO2 g–1 soil h–1 in the forest and 36.7 μl CO2 g–1 soil h–1 and 35.7 μl CO2 g–1 soil h–1 in the grassland surface soils (0–10 cm). The fungal ergosterol content in the surface soil decreased from the forest zone
(108 μg g–1) to the grassland zone (15.9 μg g–1). A good correlation (R
2=0.90) was exhibited between the soil fungal ergosterol content and soil fungal CO2 production (respiration) for all sampling sites. For the forest and grassland soil profiles, microbial biomass (respiration
and ergosterol) declined dramatically with depth, ten- to 100-fold from the surface organic horizon to the deepest mineral
horizon. With respect to fungal to bacterial ratios for the surface soil (0–10 cm), the forest zone had a significantly (P<0.05) higher ratio (1.65) than the grassland zone (1.05). However, there was no fungal to bacterial ratio trend from the surface
horizon to the deeper mineral horizons of the soil profiles.
Received: 30 March 2000 相似文献
18.
Manuel Aira Niall P. McNamara Trevor G. Piearce Jorge Domínguez 《Journal of Soils and Sediments》2009,9(1):54-61
Background, aim, and scope Earthworms make a major contribution to decomposition in ecosystems where they are present, mainly acting in the drilosphere,
that is, galleries, burrows, casts, and middens. Earthworm middens are hot-spots of microbial activity and nutrient dynamics
and represent a suitable model for studying earthworm-mediated influences on soil microbial communities by alteration of the
patch structure of the microbial environment. We studied the structure and activity of the microbial communities in the soil
system formed by middens of Lumbricus terrestris and the soil below and surrounding them and the role of earthworms in maintaining these structures through time.
Material and methods We set up an experiment in which middens were either left (control) or removed from their original place (translocated) and
left in a nearby area free of earthworm activity for 2 months. After 1 and 2 months we sampled middens, soil below them, and
surrounding soil. We analyzed the phospholipid fatty acid (PLFA) profiles and measured respiratory fluxes of CO2 and CH4.
Results Microbial communities of middens clearly differed from those of soil below and surrounding soil samples, showing higher bacterial
and fungal PLFAs (p < 0.0001 and p < 0.01, respectively); furthermore, changes in microbial communities were stronger in control middens than in translocated
middens. Moreover, gram positive and negative bacterial PLFAs were greater in translocated than control middens (p < 0.0001 and p < 0.001, respectively), as well as total organic carbon (p < 0.001). Microbial activity was higher in middens than in soil below and surrounding soil samples both for CO2 (p < 0.0001) and CH4 (p < 0.0001).
Discussion Soil bioturbation by the earthworm L. terrestris was strong in their middens, but there was not any effect on soil below and surrounding soil. Microbial communities of middens
maintain their biomass and activity when earthworms were not present, whereas they decreased their biomass and increased their
activity when earthworms were present.
Conclusions Earthworms strongly enhanced microbial activity measured as CO2 production in middens, which indicates that there are hot spots for soil microbial dynamics and increasing habitat heterogeneity
for soil microorganisms. Moreover, our data strongly support the fact that the impact of this earthworm species in this soil
is restricted to their middens and increasing soil heterogeneity.
Recommendations and perspectives Our data indicate that it is not clear if earthworms enhance or depress microbial communities of middens since the microbial
activity increased, but did not modify their biomass and this was not dependent on soil organic C content. These results indicate
no competence for C pools between this anecic earthworm and microorganisms, which has been found for other earthworm species,
mainly endogeics. Conversely, they suggest some type of facilitation due to the release of additional nutrient pools in middens
when earthworms are present, through the digestion of middens' material or the addition of casts produced from other food
sources. 相似文献
19.
Many phosphate solubilizing microorganisms (PSM) require external pyrroloquinoline quinone (PQQ) for strong phosphorus (P)
solubilization in vitro. The objective of this study was to isolate efficient and PQQ-independent PSM. A total of 21 PSM were
isolated from the rhizosphere soil of wheat and maize grown in the pots. Acinetobacter strains were the only PQQ-independent and most effective solubilizers of tricalcium phosphate containing agar. The mean P
dissolved in liquid cultures of Acinetobacter strains in a 5-day incubation ranged from 167 to 888 μg/ml P. The pH dropped to below 4.7 from 7.8 in six isolates, which
produced gluconic acid in concentrations ranging between 27.5 and 37.5 mM. There was a linear regression between soluble P
and gluconic acid concentrations in the bacterial cultures (P < 0.05; R
2 = 0.59). Inoculation with Acinetobacter sp. WR922 significantly (P < 0.05) increased wheat (Triticum aestivum L.) P content by 27% at 15 days after emergence (DAE) and dry matter by 15% at 30 DAE compared to the control. The plant
P content in inoculated plants at 30 DAE was linearly correlated with soluble P of the bacterial cultures (P < 0.05; R
2 = 0.69). Gluconic acid production directly affected phosphate solubilization in vitro, which in turn influenced plant P content
of inoculated plants in PQQ-independent P-solubilizing Acinetobacter strains. 相似文献
20.
Pot experiments were carried out over two growing periods to assay the biocontrol efficacy and rhizosphere colonization of
Trichoderma harzianum SQR-T037 (SQR-T037) applied as SQR-T037 conidia suspension (TCS), SQR-T037 conidia suspension blended with organic fertilizer
(TBF), or SQR-T037 fermented organic fertilizer (TFF). Each formulation had three T. harzianum numbers. In two experiments, Percent Disease Indexes (PDIs) decreased with the increase of SQR-T037 number added to soils.
The TFF treatment consistently exhibited the lowest PDIs at same amendment rate of SQR-T037 and 0–8.9%, 25.6–78.9%, and 4.4–50.0%
of PDIs were found in TFF, TCS, and TBF treatment, respectively. Soils treated with TFF showed the highest SQR-T037 population
in rhizosphere and bulk soil. Decrease of Fusarium oxysporum population in both bulk and rhizosphere soils occurred in the treatment SQR-T037 at 105 and 106 cfug−1 soil rate. The TFF treatment at the SQR-T037 rate of 103 cfug−1 soil significantly (p < 0.05) increased SQR-T037 population within the rhizoplane but had no effect on F. oxysporum population when compared to TCS and TBF. Generally, TFF treatments were superior to TCS and TBF treatments on disease control
by sustaining colonization of SQR-T037 and decreasing F. oxysporum abundance in the rhizosphere soil. We propose that TFF treatment at SQR-T037 rate of 107 cfug−1 (i.e., 105 cfug−1 soil after applied to soil) was the best formulation for controlling Fusarium wilt of cucumber. 相似文献