A bibliography and review of genetic diversity studies of African germplasm using protein and DNA markers

A review of the existing literature available on the bibliographic CD-ROM PlantGeneCD on the use of protein and molecular markers on plant material from sub-Saharan Africa was undertaken. The review showed that these molecular techniques have been used in a wide range of studies, from measurement of genetic diversity within single population to the elucidation of phylogenetic relationship among species in a genus or group of genera. The review revealed that 67% of the 146 studies examined used protein electrophoresis (58% isozymes, the rest mostly seed proteins), 20% used RFLPs, 8% RAPDs and 3% sequencing. Isozymes have been used for a whole range of applications, whereas electrophoresis of other proteins has mainly been used to study ecogeographic distribution of diversity in crops. RAPDs have been used particularly in the investigation of genetic relationship among accessions of a single species and phylogentic relationship among species. RFLPs of cpDNA have mostly been used at the interspecific level and nuclear markers at the intraspecific level. The studies covered most of the major crop species of Africa and their wild relatives, though important gaps remain, for example sweet potato. So-called minor crops were not well represented. Wild taxa not related to crops were also covered, in particular agroforestry and forest species.     

Multivariate analysis of morphological variation in sorghum (Sorghum bicolor (L.) Moench) germplasm from Ethiopia and Eritrea

Multivariate methods, including principal component, cluster and discriminant analyses, were used to assess the patterns of morphological variation and to group 415 sorghum accessions for 15 quantitative characters. The first five principal components explained 79% of the total variation with plant height and days to 50% flowering being the most important characters in the first principal component. Cluster analysis grouped the accessions into ten clusters. A greater proportion of accessions of similar adaptation zones and accessions from regions of origin with similar agro-climatic conditions were grouped together. Moreover, discrimination of accessions was more pronounced when discriminant analysis was based on zone of adaptation rather than regions of origin. Based on the observed patterns of variation, it is concluded that the morphological variation in the material studied is structured by environmental factors. The implications of the results for plant breeding and germplasm conservation programmes arediscussed.     

Results of activities to maintain landraces and other material in some European countries in situ before 1945 and what we may learn from them

All three recommendations to maintain landraces in European countries, given in 1927: 1. maintenance by traditional farmers, 2. maintenance by school personnel and pupils, and 3. maintenance by small agricultural institutions, were disregarded. The present outcome: maintenance by genebanks, which often grow landraces and improved cultivars of one crop next to each other, and which often are part of a large agricultural research institute, was advised against.The above observation may be of value to those who at present advocate maintenance of landraces by traditional farmers in developing countries.It is recommended that landraces should be collected and maintained in genebanks as this will result in a partial loss of the material, whereas on farm maintenance (in situ conservation) would lead to a complete loss.     

Soil landscapes,land cover change and erosion features of the Central Plateau region of Tigrai,Ethiopia: Photo-monitoring with an interval of 30 years

Human land use of the Tigray landscape (north Ethiopia) can be traced back for at least 3000 years and is recognizably very complex, but in the past half-century there have been multiple narratives on environmental change in the Northern Ethiopian Highlands in which statements such as “the forest and soil resources in Tigray are dwindling at unprecedented rates” are common. In an attempt to provide an objective assessment, we made a semi-quantitative analysis of observed changes in the environment of the central Tigray plateau, between 1975 and 2006, and its impact on soil erosion. The first part of this period saw strong degradation, caused by a combination of drought, impoverishment, poor land husbandry and war; but over the whole period intense rehabilitation activities have been high on the agenda. To study these changes, two sets of 51 landscape photographs have been used. The older photo-set was taken in 1975 by R.N. Munro during the Tigrai Rural Development Study; locations were revisited in 2006 by J. Nyssen and colleagues, when a new set of photographs was made at the same locations and with the same aspect. Based on longstanding experience in soil erosion and landscape analysis worldwide and in Ethiopia, the time-lapsed photographs were rated for visible erosion, land cover and protective measures. We present a quantitative evaluation of the change of soil loss by sheet and rill erosion, involving the Universal Soil Loss Equation (USLE) and particularly the changes in the C (cover) and the P (management) factors. This allowed assessing soil loss in 2006 as a percentage of the 1975 situation. Both the landscape and land unit analysis show that the situation for natural resources has improved (and locally strongly improved) since 1974. The rehabilitation is due both to improved vegetation cover and to physical conservation structures. The USLE application indicates that in terms of a whole landscape the current average soil loss would be at around 68% of its 1975 rate. Exceptionally, degradation is still ongoing around Desa'a forest and some other remnant forests, and conservation should be strongly implemented too in these forests. On average, gullies have expanded slightly since 1975, but these incisions appear to have originated in the drought years of the 1980s. This photo-monitoring analysis invalidates hypotheses on (a) irreversibility of land degradation in Tigray; and (b) futility of Soil and Water Conservation (SWC) programmes. The study demonstrates that (a) land management has become an inherent part of the farming system in Tigray, and (b) that the authorities and NGOs are on the right track when promoting SWC.     

The Vigna angularis complex: Genetic variation and relationships revealed by RAPD analysis, and their implications for in situ conservation and domestication

The present study, using RAPD analysis, was undertaken to characterize genetic variation in three forms of V. angularis, cultivated, wild and weedy forms, and their relationships. The materials used consisted of 171 individuals (plants) or cultivars from 23 populations including 5 wild populations, 6 weedy populations, 6 cultivated populations and 6 populations with plants having wild and weedy or intermediate morphology, denoted here as complex populations. The materials used were collected on Honshu Island, Japan and seeds collected directly from the field were germinated for DNA extraction. In addition, 6 landrace accessions of V. angularis from the genebank were also analyzed. Genetic variation was highest in the wild form (H_g= 0.132; GD = 0.388), followed by the weedy form (H_g= 0.124; GD = 0.341) and the least in the cultivated form (H_g= 0.079; GD = 0.274). Intra-population genetic variation was high in the weedy and in the wild populations. However, inter-population was greater than intra-population genetic variation for all groups of populations studied in the V. angularis complex. 93% of the total diversity in the present study was exhibited by plants from complex populations and specific RAPD bands were found in these populations. Our results provide evidence that complex populations would be a logical focus for efforts to conserve the V. angularis complex in situ. Our results suggest that weedy populations are usually an ecotype of the wild form adapted to a different habitat.     

Application of fragmentation research to conservation planning for multiple stakeholders: An example from the Taita Hills, southeast Kenya

Attrition and fragmentation of natural habitats remains one of the most persistent problems for conservationists. Exactly how habitat loss leads to the loss of biodiversity is not easily pinned down, and varies across species, taxonomic groups and landscapes. Integrating theory with conservation on the ground is challenging due to the multi-faceted nature of the problem. Here, we briefly highlight and review key scientific results from an ongoing long-term ornithological research project in the highly fragmented and threatened forests of the Taita Hills, SE Kenya. The findings point to the inability of birds to disperse between remnant habitats, or to adjust to changing habitat quality as the main mechanisms underlying adverse effects of forest loss on birds. We integrated our findings and their implications for conservation in a recent stakeholders’ workshop bringing together scientists, government officials and members of the local community in the Taita Hills. During this workshop the local community and the government voiced their priorities concerning these forest resources, by identifying the pressing problems and suggesting best avenues of mitigating them. We discuss how the scientific results and recommendations from a biodiversity standpoint are being translated into actual conservation, taking into account the views and priorities of other key stakeholders. This exemplifies one of the few unique situations in eastern Africa where enormous effort is being made towards bridging the chronic gap between scientists, government and local communities concerning the conservation of natural resources. We believe that this approach holds great promise for achieving lasting conservation on the ground.     

Centres of Crop Diversity and/or Origin, Genetically Modified Crops and Implications for Plant Genetic Resources Conservation

The concept of centres of crop diversity and/or origin of agriculture is briefly reviewed. The conservation status of crop genetic resources, either ex situ or in situ, cultivated or wild, has been assessed for species of the Central American and Mexican centre, demonstrating that that region is indeed one of the important centres of crop diversity for human kind. Furthermore, biotechnological developments with regard to the creation and spread of genetically modified crops have been analyzed. The likelihood of unintentional introgression of genetically modified traits into conventional seed lots, crops as well as into germplasm collections have been assessed. Related biosafety measures as well as the possible implications of intellectual property rights on transgenic crops and/or genes are being discussed vis-a-vis the possible implications they might have for germplasm management. The Central American crop genetic resources situation has been used as a “case study” to illustrate the potential impact of the spreading of GM varieties on the genetic diversity in genebanks and farmers’ fields and the need for effective and efficient conservation efforts. Conservation management strategies and practices are being proposed of mitigate the potential negative impact of GM crops on the conservation efforts.     

Changes in food resources and conservation of scarab beetles: from sheep to dog dung in a green urban area of Rome (Coleoptera, Scarabaeoidea)

The aim of the research was to show how a change in land use influences the structure of a dung beetle assemblage and affect its conservation. In the Pineto Urban Regional Park (Rome), dog dung is the sole food resource currently available for scarab dung beetles, after the recent removal of wild and domestic herbivores. A one-year sampling was conducted to study the scarab assemblage in dog scats (1999) and to compare it with the previous assemblage associated with sheep droppings (1986). Richness, evenness and similarity parameters were compared between the two allochronic assemblages. From sheep to dog dung, an impoverishment of the total richness was observed (from 19 to 9 species) together with an increase of individuals (by 7 times). Dog dung harboured 20% of the current scarab dung beetle fauna of Rome, probably as a consequence of the dog mixed diet, rich in cellulose. Both the communities showed a high percentage of tunnellers, probably because of the food shortage and, for dog scats, of the high dehydration rate. A comparison with other Roman scarab communities enhanced that: (1) the change in food resource determined a higher difference in species composition respect to other parameters (size and habitat diversity); (2) dog dung provided a temporary refuge for species that otherwise may encounter local extinction in urban environments.     

Behavioural response of a trophic specialist, the Iberian lynx, to supplementary food: Patterns of food use and implications for conservation

Prey scarcity compromises population survival, especially for specialist predators. Supplementary feeding is a management tool that can be applied to reverse the decline of food-limited populations. We analyse how a population of Iberian lynx, a threatened food specialist, initially reacted to, and subsequently used, supplementary food. Twenty-seven feeding stations (FS) with domestic rabbits were placed in the Doñana Biological Reserve, SW Spain, between 2002 and 2005. We recorded lynx tracks inside and around stations to analyse spatio-temporal patterns of use, as well as the performance of several station designs. Iberian lynx used 62% of the FS installed, and consumed most of the supplied food. All station designs were used and food provision apparently covered the energetic needs of the lynx inhabiting the reserve. There was spatial aggregation in the use of the FS. Fourteen weeks were needed on average for lynx to become familiar with feeding stations and making a regular use of the supplementary food. Seasonal variations in consumption frequency appeared to be modulated by fluctuations of wild rabbit numbers throughout its annual cycle as well as by variation in energy demand of breeding females. The Iberian lynx responded positively to our supplementary feeding programme. We show that this technique allows the persistence of lynx populations during long periods in areas where wild rabbits are extremely scarce. Supplementary food may be used to sustain lynx whenever rabbit populations need recovery, as well as in the context of lynx restocking or reintroduction programmes.