南亚热带三种不同土地利用历史的森林生态系统土壤可交换阳离子对模拟氮沉降的响应

The effects of simulated nitrogen (N)deposition on soil exchangeable cations were studied in three forest types of subtropical China. Four N treatments with three replications were designed for the monsoon evergreen broadleaf forest (mature forest):control (0 kg N ha^-1 year^-1), low N (50 kg N ha^-1 year^-1), medium N (100 kg N ha^-1 year^-1)and high N (150 kg N ha^-1 year^-1), and only three treatments (i.e., control, low N, medium N)were established for the pine and mixed forests. Nitrogen had been applied continuously for 26 months before the measurement. The mature forest responded more rapidly and intensively to N additions than the pine and mixed forests, and exhibited some significant negative symptoms, e.g., soil acidification, Al mobilization and leaching of base cations from soil. The pine and mixed forests responded slowly to N additions and exhibited no significant response of soil cations. Response of soil exchangeable cations to N deposition varied in the forests of subtropical China, depending on soil N status and land-use history.     

Responses of Soil Acid Phosphomonoesterase Activity to Simulated Nitrogen Deposition in Three Forests of Subtropical China

Soil acid phosphomonoesterase activity(APA)plays a vital role in controlling phosphorus(P)cycling and reflecting the current degree of P limitation.Responses of soil APA to elevating nitrogen(N)deposition are important because of their potential applications in addressing the relationship between N and P in forest ecosystems.A study of responses of soil APA to simulated N deposition was conducted in three succession forests of subtropical China.The three forests include a Masson pine(Pinus massoniana)forest (MPF)-pioneer community,a coniferous and broad-leaved mixed forest(MF)-transition community and a monsoon evergreen broad-leaved forest(MEBF)-climax community.Four N treatments were designed for MEBF:control(without N added),low-N(50 kg N ha^-1 year^-1),and medium-N(100 kg N ha^-1 year^-1)and high-N(150 kg N ha^-1 year^-1),and only three N treatments(i.e.,control, low-N,medium-N)were established for MPF and MF.Results showed that soil APA was highest in MEBF,followed by MPF and MF.Soil APAs in both MPF and MF were not influenced by low-N treatments but depressed in medium-N treatments.However,soil APA in MEBF exhibited negative responses to high N additions,indicating that the environment of enhanced N depositions would reduce P supply for the mature forest ecosystem.Soil APA and its responses to N additions in subtropical forests were closely related to the succession stages in the forests.     

施氮、供水对菠菜(Spinacia oleracea L.)的生长和土壤残留矿质氮的影响

Effects of conventional and optimized water and nitrogen managements on spinach (Spinacia oleracea L.) growth and soil mineral N (N_min) residues were compared in an open field experiment in which water balancemethod and N recommendation with the KNS-system were included. It was shown that the conventionalwater treatment (seasonal irrigated amount: 175 mm) reduced spinach growth compared to the water balancetreatments (seasonal irrigated amount: 80 and 85 mm) at the same N supply level due to N loss through leaching caused by excessive water supply. Although 309 kg N ha^-1 was applied in the conventional N treatment, compared to 82 and 66 kg N ha^-1 in the optimum N treatments, no significant difference in cropyield was investigated between the N treatments with the same irrigation practice. N uptake in spinach andsoil residual Nmin contents were also significantly affected by the irrigation practices. The conventional water supply not only decreased water use efficiency, but also resulted in excessive NO₃^--N being leached below the root zone. In order to meet the same target value of N requirement for the next crop, cauliflower, based on the KNS-system, at least extra 50 kg N ha^-1 was needed in the conventional water treatments in comparison to the water balance treatment.     

保护地蔬菜轮作体系中两种不同复合肥对产量的影响及其氮素动态特征

Yield and N uptake of tomato (Lycopersicum esculentum Mill.) and pepper (Capsicum annuum L.) crops in five successive rotations receiving two compound fertilizers (12-12-17 and 21-8-11 N-P₂O₅-K₂O) were studied to determine 1) crop responses, 2) dynamics of NO₃-N and NH₄-N in different soil layers, 3) N balance and 4) system-level N efficiencies. Five treatments (2 fertilizers, 2 fertilizer rates and a control), each with three replicates, were arranged in the study. The higher N fertilizer rate, 300 kg N ha^-1 (versus 150 kg N ha^-1), returned higher vegetable fruit yields and total aboveground N uptake with the largest crop responses occurring for the low-N fertilizer (12-12-17) applied at 300 kg N ha^-1 rather than with the high-N fertilizer (21-8-11). Ammonium-N in the top 90 cm of the soil profile declined during the experiment, while nitrate-N remained at a similar level throughout the experiment with the lower rate of fertilizer N. At the higher rate of N fertilizer there was a continuous NO₃-N accumulation of over 800 kg N ha^-1. About 200 kg N ha^-1 was applied with irrigation to each crop using NO₃-contaminated groundwater. In general, about 50% of the total N input was recovered from all treatments. Pepper, relative to tomato, used N more efficiently with smaller N losses, but the crops utilized less than 29% of the fertilizer N over the two and a half-year period. Local agricultural practices maintained high residual soil nutrient status. Thus, optimization of irrigation is required to minimize nitrate leaching and maximize crop N recovery.     

过去20年中国稻田土壤有机碳变化的模拟研究

Changes in soil organic carbon (SOC) of rice paddies in China were simulated from 1980 to 2000 by linking a coupled bio-physical model to GIS database. The coupled model consists of two sub-models including Crop-C for simulating net primary productivity and hence residue retention and Soil-C for computing the turnover rates of SOC. The GIS database included parameters of climate, soils and agricultural activities with the resolution of 10 km × 10 km. Model simulation indicated that Chinese rice paddies covering 22.6 Mha sequestrated a considerable amount of C, about 0.15 ± 0.07 Pg C from 1980 to 2000. Annual sequestration rate increased sharply from -180 ± 45 kg C ha^-1 year^-1 in 1980 to 440 ± 170 kg C ha^-1 year^-1 in 1989. Thereafter, a steady sequestration rate of 460 ± 170 kg C ha^-1 year^-1 occurred till 1994 and declined since then. Approximately 84% of the Chinese rice paddies sequestrated carbon, while 15% lost carbon and 1% kept balance over the 20 years. Great SOC sequestration occurred in eastern, southern and central China, while a slight decline of SOC existed in some regions of northeastern and southwestern China.     

中国南方红壤上作物产量和土壤性质对长期施肥的响应

A 15-year fertilization experiment with different applications of inorganic N, P and K fertilizers and farmyard manure (M)was conducted to study the yield and soil responses to long-term fertilization at Qiyang, Hunan Province, China. Average grain yields of wheat and corn (1 672 and 5 111 kg ha^-1, respectively)for the treatment NPKM were significantly higher than those (405 and 310 kg ha^-1)of the unfertilized control and single inorganic fertilizer treatments. Compared with the corresponding initial values of the experiment, all treatments showed a yield decline of 9 to 111 kg ha^-1 year^-1 in wheat and 35 to 260 kg ha^-1 year^-1 in corn, respectively, and a significant pH decline of 0.07 to 0.12 pH year^-1, except for the treatments PK and NPKM. After long-term fertilization, the soil organic C, soil available P, exchangeable Ca²⁺ and Mg²⁺ and available Cu²⁺ and Zn²⁺ contents were higher in the treatment NPKM than in the treatments applied with inorganic fertilizer only. Compared to the treatment NPK, the treatment NPKM, where manure partially replaced inorganic N, had a positive impact on arresting the decline of soil pH. This improved grain yields of wheat and corn, suggesting that application of NPK fertilizer in combination with farmyard manure is important to maintain soil fertility and buffering capacity in red soil.     

施用辐照处理的污水污泥对作物产量和土壤氮的影响

A field experiment was conducted to study the feasibility of irradiated and non-irradiated sewage sludge as a fertilizer for the growth of wheat and rice. The irradiated and non-irradiated sewage sludge were applied at rates of 0 (CK), 75, 150, 225 and 300 kg N ha^-1 for wheat, and 0 (CK), 112.5, 225, 337.5 and 450 kg N ha^-1 for rice, respectively. (NH₄)₂SO₄ at a rate of 150 kg N ha^-1 for wheat, and 225 kg N ha^-1 for rice were added to the control treatments. Additionally, 20 kg ¹⁵N ha^-1 in the form of (NH₄)₂SO₄ was added to each treatment for wheat to study the effect of sewage sludge on chemical nitrogen fertilizer recovery. The results showed that the irradiation of sewage sludge by gamma ray at a dosage of 5 kGy increased crop yield by 11%~27% as compared to the non-irradiated treatments. Irradiation stimulated mineralization of organic nitrogen in the sludge and improved seedling growth. It was found that addition of irradiated sludge could reduce the leaching loss of chemical nitrogen fertilizer. Both irradiated and non-irradiated sewage sludge could increase the content of soil total nitrogen. Based on the preliminary results, it was concluded that irradiated sewage sludge could partly substitute for chemical nitrogen fertilizer in crop production.     

华北平原冬小麦-夏玉米轮作体系中标记15N的去向及残效

A field experiment was conducted to investigate the fate of ¹⁵N-labeled urea and its residual effect under the winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) rotation system on the North China Plain. Compared to a conventional application rate of 360 kg N ha^-1 (N360), a reduced rate of 120 kg N ha^-1 (N120) led to a significant increase (P < 0.05) in wheat yield and no significant differences were found for maize. However, in the 0-100 cm soil profile at harvest, compared with N360, N120 led to significant decreases (P < 0.05) of percent residual N and percent unaccounted-for N, which possibly reflected losses from the managed system. Of the residual fertilizer N in the soil profile, 25.6%-44.7% and 20.7%-38.2% for N120 and N360, respectively, were in the organic N pool, whereas 0.3%-3.0% and 11.2%-24.4%, correspondingly, were in the nitrate pool, indicating a higher potential for leaching loss associated with application at the conventional rate. Recovery of residual N in the soil profile by succeeding crops was less than 7.5% of the applied N. For N120, total soil N balance was negative; however, there was still considerable mineral N (NH₄⁺-N and NO₃^--N) in the soil profile after harvest. Therefore, N120 could be considered agronomically acceptable in the short run, but for long-term sustainability, the N rate should be recommended based on a soil mineral N test and a plant tissue nitrate test to maintain the soil fertility.     

南亚热带三种典型植被土壤动物对模拟N沉降增加的早期响应

A field-scale experiment arranged in a complete randomized block design with three N addition treatments including a control （no addition of N）, a low N （5 g m^-2 year^-1）, and a medium N （10 g m^-2 year^-1） was performed in each of the three typical forests, a pine （Pinus massoniana Lamb.） forest （PF）, a pine-broadleaf mixed forest （MF） and a mature monsoon evergreen broadleaf forest （MEBF）, of the Dinghushan Biosphere Reserve in subtropical China to study the response of soil fauna community to additions of N. Higher NH4^＋ and NO3^- concentrations and a lower soil pH occurred in the medium N treatment of MEBF, whereas the NO3^- concentration was the lowest in PF after the additions of N. The response of the density, group abundance and diversity index of soil fauna to addition of N varied with the forest type, and all these variables decreased with increasing N under MEBF but the trend was opposite under PF. The N treatments had no significant effects on these variables under MF. Compared with the control plots, the medium N treatment had significant negative effect on soil fauna under MEBF. The group abundance of soil fauna increased significantly with additions of higher N rates under PF. These results suggested that the response of soil fauna to N deposition varied with the forest type and N deposition rate, and soil N status is one of the important factors affecting the response of soil fauna to N deposition.     

尼日利亚两个农业生态区施用氮肥和杂草干扰对热带玉米基因型的影响

Low soil nitrogen (N) and weed infestations are some of the major constraints to maize production in Nigeria.A split-split plot experiment in a randomized complete block design with three replicates was established at two sites with different agroecological zones,Ikenne (Typic Paleudalf) and Shika (Typic Tropaquept),in Nigeria in 2002 and 2003 rainy seasons to investigate the responses of four maize genotypes (Oba super II,Low N pool C2,TZB-SR,and ACR 8328 BN C7) to N fertilizer applied at four rates,0,30,60,and 90 kg N ha^-1,and three weed pressure treatments,no weed pressure (weekly weeding),low weed pressure (inter-row weekly weeding),and high weed pressure (no weeding throughout the growing season).Growth and yield parameters of maize and weeds were taken at flwering and harvest.The results indicated that there was a significant reduction in maize leaf area,leaf area index,and photosynthetically active radiation due to weed interference at both sites.The application of nitrogen at 90 kg N ha^-1 significantly increased maize leaf area.Reductions in maize growth and yield at flowering and harvest were significant due to weed interference at both Ikenne and Shika,thus showing that the reductions in maize growth and yield due to weed interference were not ecological zone specific even though weed species and their seed banks may differ.Ameliorative management options could thus be the same in the two agroecological zones.Application of 90 kg N ha^-1 led to a significant increase in maize grain yield at Shika while there was no fertilizer effect at Ikenne on grain yield.There was no significant difference between 60 and 90 kg N ha^-1,suggesting that 60 kg N ha^-1 could be a possible replacement for the higher fertilizer rate at least for the identified maize genotypes.Low weed pressure treatment led to 26% and 35% reductions in maize grain yield at Ikenne and Shika,respectively,while 22% and 51 % reductions,respectively,were observed due to high weed pressure.Generally,maize grain yield was higher at Ikenne than Shika.The maize genotypes Low N pool C2 and ACR 8328 BN C7 performed better than the other genotypes at Ikenne while the maize genotype Oba super II had the best performance at harvest at Shika.Application of nitrogen increased weed biomass at flowering at Ikenne.The maize grain yield was highest in the N-efficient genotypes,Oba super II and Low N pool C2;the susceptible genotype TZB-SR had the least yield at Shika.There existed a negative and significant correlation between maize grain yield and weed biomass at both sites.     

Effect of Simulated N Deposition on Soil Exchangeable Cations in Three Forest Types of Subtropical China

The effects of simulated nitrogen (N) deposition on soil exchangeable cations were studied in three forest types of subtropical China.Four N treatments with three replications were designed for the monsoon evergreen broadleaf forest (mature forest):control (0 kg N ha-1 year-1),low N (50 kg N ha-1 year-1),medium N (100 kg N ha-1 year-1) and high N (150 kg N ha-1 ycar-1),and only three treatments (i.e.,control,low N,medium N) were established for the pine and mixed forests.Nitrogen had been applied continuously for 26 months before the measurement.The mature forest responded more rapidly and intensively to N additions than the pine and mixed forests,and exhibited some significant negative symptoms,e.g.,soil acidification,Al mobilization and leaching of base cations from soil.The pine and mixed forests responded slowly to N additions and exhibited no significant response of soil cations.Response of soil exchangeable cations to N deposition varied in the forests of subtropical China,depending on soil N status and land-nse history.     

中国南方大棚蔬菜地氮平衡与损失

High rates of fertilizer nitrogen (N) are applied in greenhouse vegetable fields in southeastern China to maximize production;however,the N budgets of such intensive vegetable production remain to be explored.The goal of this study was to determine the annual N balance and loss in a greenhouse vegetable system of annual rotation of tomato,cucumber,and celery at five N (urea) application rates (0,348,522,696,and 870 kg N ha-1 year-1).Total N input to the 0-50 cm soil layer ranged from 531 to 1 053 kg ha-1,and N fertilizer was the main N source,accounting for 66%-83% of the total annual N input.In comparison,irrigation water,wet deposition,and seeds in total accounted for less than 1% of the total N input.The fertilizer N use efficiency was only 18% under the conventional application rate of 870 kg N ha-1 and decreased as the application rate increased from 522 to 870 kg N ha-1.Apparent N losses were 196-201 kg N ha-1,of which 71%-86% was lost by leaching at the application rates of 522-870 kg N ha-1.Thus,leaching was the primary N loss pathway at high N application rates and the amount of N leached was proportional to the N applied during the cucumber season.Moreover,dissolved organic N accounted for 10% of the leached N,whereas NH3 volatilization only contributed 0.1%-0.6% of the apparent N losses under the five N application rates in this greenhouse vegetable system.     

Synthesis of Nitrogen Pools and Fluxes from European Forest Ecosystems

To investigate which ecosystem parameters determine the risk and magnitude of nitrate leaching we compiled data from published and unpublished sources on dissolved inorganic nitrogen (DIN: NO₃ ^-) in throughfall, DIN leaching loss in runoff or seepage water, and other ecosystem characteristics from 139 European forests. Not all data were available for all sites: 126 sites had at least one year's data on DIN inputs and DIN leaching loss; 40-50 sites had some data on soil chemistry and/or vegetation pools of N. DIN inputs in throughfall range between <1 and about 70 kg N ha-1 yr-1 and the losses with seepage or runoff range between <1 and 50 kg N ha-1 yr-1. Retention of N within the ecosystem increases with increasing DIN deposition and increasing proportion of NH₄ ⁺ in deposition. The amount of N in needles and litterfall shows a significant linear relationship with throughfall deposition of DIN, whereas the C:N ratio of the organic (OH) horizon is uncorrelated to the level of throughfall-DIN flux. About 50% of the variability in DIN leaching loss can be explained by the flux of DIN in throughfall. Alternatively, about 60% of the variability in DIN leaching loss can be explained in a two-variable multiple regression combining the C:N ratio of the organic soil and the pH of the mineral soil. The survey data suggest that leaching of DIN from forest ecosystems in Europe is related in part to current DIN deposition and in part to the longer-term internal ecosystem N status as reflected in the chemistry of the humus and acidification status of the soil.     

太湖地区水稻最适宜施氮量研究

To determine the optimal amount of nitrogen(N) fertilizer for achieving a sustainable rice production at the Taihu Lake region of China,two-year on-farm field experiments were performed at four sites using various N application rates.The results showed that 22%-30% of the applied N was recovered in crop and 7%-31% in soils at the rates of 100-350 kg N ha 1.Nitrogen losses increased with N application rates,from 44% of the applied fertilizer N at the rate of 100 kg N ha 1 to 69% of the N applied at 350 kg N ha 1.Ammonia volatilization and apparent denitrification were the main pathways of N losses.The N application rate of 300 kg N ha 1,which is commonly used by local farmers in the study region,was found to lead to a significant reduction in economic and environmental efficiency.Considering the cost for mitigating environmental pollution and the maximum net economic income,an application rate of 100-150 kg N ha 1 would be recommended.This recommended N application rate could greatly reduce N loss from 199 kg N ha 1 occurring at the N application rate of 300 kg N ha 1 to 80-110 kg N ha 1,with the rice grain yield still reaching 7 300-8 300 kg DW ha 1 in the meantime.     

太湖地区稻麦轮作下氮素径流和淋洗损失

Although nitrogen （N） loss through runoff and leaching from croplands is suspected to contribute to the deterioration of surrounding water systems, there is no conclusive evidence for paddy soils to prove this hypothesis. In this study, field plot experiments were conducted to investigate N losses through runoff and leaching for two consecutive years with 3 N fertilization rates in rice （Oryza sativa L.）-wheat （Triticum aestivum L.） rotations in the Taihu Lake region, China. A water collection system was designed to collect runoff and leachates for both the rice and wheat seasons. Results showed that dissolved N （DN）, rather than particulate N （PN）, was the main form of N loss by runoff. The NO3^--N concentration in runoff was between 0.1 and 43.7 mg L^-1, whereas the NH4^＋-N concentration ranged from below detection limit to 8.5 mg L^-1. Total N （TN） loads by runoff were 1.0-17.9 and 5.2-38.6 kg ha^-1 during rice and wheat seasons, respectively, and the main loss occurred at the early growing stage of the crops. Nitrogen concentrations in leachates during the rice seasons were below 1.0 mg L^-1 and independent of the N application rate, whereas those during the wheat season increased to 8.2 mg L^-1 and were affected by the fertilizer rate. Annual losses of TN through runoff and leaching were 13.7-48.1 kg ha^-1 from the rice-wheat cropping system, accounting for 5.6%-8.3% of the total applied N. It was concluded that reduction in the N fertilization rate, especially when the crop was small in biomass, could lower the N pollution potential for water systems.     

中国中亚热带杉树及常绿阔叶林中焚烧对养分流失及土壤肥力的影响

A Chinese fir forest(Cunninghamia lanceolata,CF) and an evergreen broadleaved forest(EB) located in Fujian Province,southeastern China,were examined following slash burning to compare nutrient capital and topsoil properties with pre-burn levels.After fire,nutrient(N,P and K) removal from burining rsidues was estimated at 302.5kg ha^-1 in the CF and 644.8kg ha^-1 in the EB.Fire reduced the topsoil capitals of total N and P by about 20% and 10%,respectively,in both forests,while K capital was incresed in the topsoils of both forests following fire.Total site nutrient loss through surface erosion was 28.4kg(N) ha^-1 ,84.kg(P)ha^-1 and 328.7kg(K) ha^-1 in the CF.In the EB,the losses of total N,P and K were 58.5,10.5 and 396.3kg ha^-1,respectively,Improvement of soil structure and increase in mineralization of nutrients associated with increasesd microbe number and enzyme acitvityes and elecvated soil respiration occurred 5 day after fire.Howerver,organic matter and available nutrient contents and most of other soil parameters declined ont year after fire on the burned CF and EB topsoils.These rsults suggest that short-tmerm site productivity can be stimulated immediately.but reduced subsequently by soil and water losses,especially in south China,where high-intensity precipitation,steep slopes and fragile soil can be expected,Therefore,the silvicultural measurements should be developed in plantation management.     

提高高产玉米氮素利用效率的根层氮素管理技术

Many recently developed N management strategies have been extremely successful in improving N use efficiency.How-ever,attempts to further increase grain yields have had limited success.Field experiments were conducted in 2007 and 2008 at four sites to evaluate the effect of an in-season root-zone N management strategy on maize (Zea mays L.).According to the in-season root-zone N management,the optimal N rate (ONR) was determined by subtracting measured soil mineral N (NH + 4--N and NO 3--N) in the root zone from N target values.Other treatments included a control without N fertilization,70% of ONR,130% of ONR,and recommended N rate (RNR) by agronomists in China that have been shown to approach maize yield potentials.Although apparent N recovery for the ONR treatment was significantly higher than that under RNR in 2007,grain yield declined from 13.3 to 11.0 Mg ha 1 because of an underestimation of N uptake.In 2008,N target values were adjusted to match crop uptake,and N fertilization rates were reduced from 450 kg N ha-1 for RNR to 225 to 265 kg N ha-1 for ONR.High maize yields were maintained at 12.6 to 13.5 Mg ha 1,which were twice the yield from typical farmers’ practice.As a result,apparent N recovery increased from 29% to 66%,and estimated N losses decreased significantly for the ONR treatment compared to the RNR treatment.In conclusion,the in-season root-zone N management approach was able to achieve high yields,high NUE and low N losses.     

Effects of nitrogen deposition on soil organic carbon fractions in the subtropical forest ecosystems of S China

Experiments were conducted between 2003 and 2008 to examine how N additions influence soil organic C (SOC) and its fractions in forests at different succession stages in the subtropical China. The succession stages included pine forest, pine and broadleaf mixed forest, and old‐growth monsoon evergreen broadleaf forest. Three levels of N (NH₄NO₃)‐addition treatments comprising control, low‐N (50 kg N ha^–1 y^–1), and medium‐N (100 kg N ha^–1 y^–1) were established. An additional treatment of high‐N (150 kg N ha^–1 y^–1) was established in the broadleaf mixed forest. Soil samples were obtained in July 2008 for analysis. Total organic C (TOC), particulate organic C (POC, > 53 μm), readily oxidizable organic C (ROC), nonreadily oxidizable organic C (NROC), microbial biomass C (MBC), and soil properties were analyzed. Nitrogen addition affected the TOC and its fractions significantly. Labile organic‐C fractions (POC and ROC) in the topsoil (0–10 cm) increased in all the three forests in response to the N‐addition treatments. NROC within the topsoil was higher in the medium‐N and high‐N treatments than in the controls. In the topsoil profiles of the broadleaf forest, N addition decreased MBC and increased TOC, while no significant effect on MBC and TOC occurred in the pine and mixed forests. Overall, elevated N deposition increased the availability of labile organic C (POC and ROC) and the accumulation of NROC within the topsoil irrespective of the forest succession stage, and might enhance the C‐storage capacity of the forest soils.