Are Nitric Acid (HNO<Subscript>3</Subscript>) Digestions Efficient in Isolating Microplastics from Juvenile Fish?

A standard method for the detection and isolation of microplastics is required to adequately investigate plastic ingestion by juvenile fish. Dissections of juvenile fish guts require precise handling, which can affect the processing time if sample numbers are high. To investigate the efficacy of nitric acid (HNO₃) in aiding the isolation of microplastics using whole fish, we digested juvenile glassfish, Ambassis dussumieri (Cuvier, 1828), at room temperature and at 80 °C. For a complete digestion, overnight incubation in 10 mL of 55% analytical-reagent (AR) HNO₃ was sufficient for a whole fish of 1 g at room temperature. When coupled with elevated temperature, the digestion time is shortened to a few minutes and larger fish of 3 g can be digested in 30 min. Four of the five types of plastic survived the process, with nylon being the exception. This is a shortfall to the method; however, until a better method replaces it, we still value the use of HNO₃ for its simple, inexpensive, swift and complete digestions of whole fish. Four fish species from two feeding guilds were digested using this method to validate its use. The number of plastic particles ingested did not differ between benthic and pelagic species and microplastic fibres comprised the majority of the plastic types found.     

Changes in recovery of native and applied phosphorus with time as affected by soil properties in some calcareous soils

In an attempt to investigate variations in availability of native and applied phosphorus (P) with time, twenty five calcareous soil samples were treated with 0, 50 and 500 mg P kg^?1 soil and incubated at 80–90% of field capacity moisture content in an open door glasshouse for a period of one year. Soil samples were taken out at certain intervals for P extraction with Olsen extractant under moist condition. Results indicated that following wetting the mean value of native Olsen-P decreased initially and then increased gradually with time of incubation. Generally, the short term (24 h) recoveries were not correlated with long term ones. The recovery of added P with time was described properly by the following new proposed model: R = 100/(1 + kt^b), where R is percent recovery at time t, and k and b are empirical parameters. The values of k constant at 50 and 500 mg P kg^?1 were in the ranges of 0.40 to 1.22 (0.84 ± 0.25, on average) and 0.04 to 0.52 (0.23 ± 0.14, on average), respectively. The corresponding respective values of b constant also ranged from 0.05 to 0.32 (0.15 ± 0.06, on average) and 0.12 to 0.92 (0.46 ± 0.23, on average).     

EFFECT OF SOIL BUNDS ON RUNOFF,SOIL AND NUTRIENT LOSSES,AND CROP YIELD IN THE CENTRAL HIGHLANDS OF ETHIOPIA

The effects of soil bunds on runoff, losses of soil and nutrients, and crop yield are rarely documented in the Central Highlands of Ethiopia. A field experiment was set up consisting of three treatments: (i) barley‐cultivated land protected with graded soil bunds (Sb); (ii) fallow land (F); and (iii) barley‐cultivated land without soil bund (Bc). For 3 years (2007–2009), the effect of soil bunds on runoff, losses of soil and nutrients, and crop productivity was studied. Daily runoff and soil and nutrient losses were measured for each treatment using standard procedures while barley yield was recorded from the cultivated plots. The results showed that Sb brought about significant reduction in runoff and soil losses. Plots with Sb reduced the average annual runoff by 28 per cent and the average annual soil loss by 47 per cent. Consequently, Sb reduced losses of soil nutrients and organic carbon. However, the absolute losses were still high. This implies the need for supplementing Sb with biological and agronomic land management measures to further control soil erosion. Despite these positive impacts on soil quality, Sb do not increase crop yield. Calculated on a per‐hectare basis, Sb even reduce crop yield by about 7 per cent as compared with control plots, which is entirely explained by the reduction of the cultivable area by 8·6 per cent due to the soil bunds. Suitable measures are needed to compensate the yield losses caused by the construction of soil bunds, which would convince farmers to construct these land management measures that have long‐term beneficial effects on erosion control. Copyright © 2012 John Wiley & Sons, Ltd.     

Nitrate Removal from Waters Containing Sulfate Using Autotrophic Denitrification with Sulfide Return

In this study, a process has been proposed whereby the sulfide required for autotrophic denitrification is supplied by reducing the sulfate of influent water without the need to add an external sulfide source. The molar ratio of nitrate-to-sulfide was maintained at 1.6. The proposed system was operated continuously for 6 months, including two anoxic and anaerobic reactors with upward flow. The results indicate that the average amount of nitrate declined by 74%. The pH of 7–8 was more effective than a pH of 6 in removing the nitrate. As the hydraulic retention time was prolonged from 1.5 to 3 and was further prolonged to 5 h, the system efficiency was enhanced by removing the nitrate. An alkalinity consumption rate of 1.15 mg (as CaCO₃) per mg of removed NO₃ ^?-N was achieved. In the effluent water, the increased sulfate was 6.7 mg per mg of removed NO^? ₃-N, while the hardness was diminished by 2.85 mg (as CaCO₃).     

Cast production and NIR spectral signatures of Aporrectodea caliginosa fed soil with different amounts of half-decomposed Populus nigra litter

Sub-adult individuals of Aporrectodea caliginosa were incubated for 16 weeks under laboratory cultures in a soil treated with 0%, 10% or 50% of a Populus nigra half-decomposed leaves, respectively. Growth was maximum in the 50% organic matter treatment and cocoon production occurred. Average soil ingestion rates decreased from 1.56 g/g^?1 fresh weight of worm per day^?1 in control soil to 1.17 and 0.5 g, respectively, in treatments with 10% and 50% half-decomposed litter. Surface casts never comprised more than 10% of total cast production. Near-infrared spectrometry (NIRS)signatures of digested and non-ingested soil significantly differed and showed a rather constant effect of digestion, independent of the organic matter content (p?<?0.01). These results confirm the value of NIRS spectral signatures as indicators of the origin of soil aggregates and biological processes involved in soil aggregation.     

Nutrients management strategies to improve yield and quality of sugar beet in semi-arid regions

The objective of this study was to evaluate the effects of organic and inorganic fertilizers on the yield and quality of sugar beet genotypes (Beta vulgaris L.). Therefore, a field trial was carried out in Peshawar, Pakistan, during the winters in 2012–2013. The field experiment was conducted in a randomized complete block design with split plots, having three replications. Fertilizer treatments (control, composted manure Higo Organic Plus at 5 t ha^?1, Maxicrop Sea Gold seaweed extract at 5 L ha^?1, farm yard manure at 10 t ha^?1, inorganic nitrogen–phosphorus (NP) at 90:60 kg ha^?1, NP at 120:90 kg ha^?1 and NP at 150:120 kg ha^?1) were allotted to main plots, while genotypes (Sandrina, Serenada and Kawe Terma) were allotted to the sub-plots. Plots treated with the application of NP at 120:90 kg ha^?1 produced the highest beet yield (76.4 t ha^?1) and sugar yield (11.1 t ha^?1), and had the second highest polarizable sugar content (14.52%) and more economic return (Rs. 553,000 per hectare) as compared to control plots. Sugar beet genotype Serenada had significantly higher beet yield (55.5 t ha^?1) and sugar yield (7.9 t ha^?1) and a higher economic return (Rs. 380,000 per hectare) than the other genotypes. Sugar beet genotype Serenada supplied with NP at 120:90 kg ha^?1is recommended for the general cultivation in the agro-climatic conditions of Peshawar valley.     

Determination of Elemental Sulfur in the Presence of Anaerobic Sediments by Extraction Procedure Using High-Performance Liquid Chromatography

Elemental sulfur is the most commonly found form of sulfur in anaerobic sediments. Accurate determination of elemental sulfur is the key step to know physical chemistry and biogeochemical processes in the sediments. A novel method was developed for the analysis of elemental sulfur using high-performance liquid chromatography (HPLC) with a C18 column. The procedure of determination of elemental sulfur concentrations from 0.1 to 100 mg/g (on a basis of the dry weight of sediment) is based on the direct injection of acetone extracts of sediments into a chromatographic column. The linearity range of 20–110 mg/L showed an excellent linearity of response (r = 0.999). The limit of detection and limit of quantitation for elemental sulfur were 9.41 and 4.18 μg/L, and converted sulfur mass per sediment mass was 1.88 × 10^?2 and 8.36 × 10^?3 μg/kg. Besides, a repetitive experiment (ten times) was carried out and the average chromatographic peak area was 141.47 mg L^?1. The sulfur concentration in the solution used for the determination of standard deviation and relative standard deviation was 1.02 and 0.727%, respectively. The average recovery ranges between 98 and 100%. The quantitation of elemental sulfur in sediment samples from anaerobic digestion reactor is obvious, and the average concentration of elemental sulfur on the basis of the dry weight of the sediment is 5.24 mg/g. The method was sensitive and exhibited good signal-to-noise ratio, as well as linear responses over a wide concentration range.     

Ibuprofen Genotoxicity in Aquatic Environment: An Experimental Model Using Oreochromis niloticus

Medicines and their metabolites have been found as water contaminants at very low concentrations; moreover, there is no extensive toxicological data to determine the risks associated with their occurrence in water resources. The ibuprofen genotoxicity potential to the Oreochromis niloticus fish (Tilapia), due to nanograms per liter exposure, was evaluated using the micronucleus test. Acute (48 h) and sub-chronic assays (10 days) were carried out at 300 ng/L ibuprofen aquatic concentration comparing with the negative control group (without treatment), with eight animals per group. The results were assessed from the average of triplicate analyses. The micronucleus frequency in peripheral blood of fish was determined using a sample size of 3,000 erythrocytes per animal. Significance was defined using t test (p????0.05). The bioassay results showed a statistically significant increase in the frequency of micronuclei for both exposure times in comparison to the negative control. The micronucleus frequency observed for the sub-chronic tests was higher than the one identified in the acute assays. The observed ibuprofen genotoxic effects demonstrated an aquatic environmental risk of this pharmaceutical, which occurs for the used fish experimental model in lower concentration than previously described for other aquatic organisms.     

Behavior of Pilot-Scale Constructed Wetlands in Removing Nutrients and Sediments Under Varying Environmental Conditions

Water resources are threatened globally and declining water quality is primarily due to stormwater, agricultural, urban, and mining runoffs. Steamboat Creek in Nevada is the largest non point source (NPS) of pollution to the Truckee River. Treatment wetlands are a cost-effective and reliable technique to control NPS pollution, therefore, a large-scale wetland along Steamboat Creek has been proposed as a component of a regional watershed restoration plan. This study used ten parallel pilot-scale wetland mesocosms, and tested the effects of drying and rewetting, hydraulic retention time (HRT), and high nitrogen loading on the efficiency of nutrient and total suspended solids (TSS) removal. Drying and rewetting produced noticeable effects on nutrient retention, but the effect was short-lived. During longer HRT period nutrient removal in manipulated mesocosms with an 8 h HRT were higher than controls with a 4 h HRT. Reducing the HRT from 4 h to 30 min further decreased nutrient interception. During increased influent nitrogen loading (9.5?±?2.4 mg l^?1), manipulated mesocosms functioned as sinks for total nitrogen (TN) with removal efficiency increasing from 45?±?13% to 87?±?9%. The average change in TN concentration was 9.1?±?2.2 mg l^?1. Drying/rewetting and varying HRT influenced total phosphorus (TP) and TSS similarly, and TP removal was associated with TSS removal. Results can help make decisions regarding wetland construction, management, and operation more effective in order to reduce nutrient loads to the Truckee River.     

基于Web日志和协同过滤算法的水产养殖信息推荐

随着水产养殖信息在互联网和物联网中快速增长,大部分信息成为死信息储存于互联网中各个角落,未得到有效利用。为了从众多资源候选中选择用户需要的资源推送给用户,该文构建了一个基于Web日志的水产养殖推荐系统。该系统通过用户兴趣调查获取用户初始倾向,并运用Web日志挖掘技术构建了潜在的用户兴趣模型,将用户个性化的信息通过基于项目的协同过滤算法整合至推荐系统。基于用户的推送系统实现根据用户的需求精确推荐水产品交易、养殖技术、政府优惠政策和物联网数据等信息。     

Effect of cutting frequency on species richness and dry matter yield of permanent grassland after grazing cessation

The effect of grazing cessation management has only rarely been investigated. In this study, the influence of cutting regime in the absence of fertilization was examined during 2005–2011 on a semi-natural grassland in Rapotin (Czech Republic) which had been grazed for more than 30 years before the start of the trial. This was conducted as a randomized plot design with five levels of treatment: 4 C – intensive (four cuts per year); 3 C – medium intensive (three cuts per year); 2 C – low intensive (two cuts per year); 1 C – extensive (one cut per year); 0 C – abandoned (control; without any management). Species richness was significantly lower in treatments 1 C and 0 C than in other treatments. Management and year had similar and significant influence on species composition and explained 23% of its variability. Dry matter (DM) yields were found to be lower in three-cut than in two-cut treatments (5.56 t ha^?1 and 6.22 t ha^?1, respectively). In the case of grazing cessation, a suitable compromise of three cuts per year can be recommended to guarantee maintenance of species richness, botanical composition, and appropriate grass forage yield under similar site conditions.     

Genetic diversity in farmer grown spring barley material from Kyrgyzstan

Barley (Hordeum vulgare L.) is one of the economically important cereal crops grown in the highlands of the Kyrgyz Republic. In marginal agricultural areas of the country barley is considered as irreplaceable and very suitable fodder crop for livestock. In this study the genetic diversity of currently grown farmers’ spring barley material from Naryn and Issik-Kul provinces is described. In order to capture maximum diversity present in farmer field’s different morphological types were collected. The 22 spring barley accessions collected in 2008 were described morphologically and analysed using fourteen simple sequences repeat (SSR) markers. Most material was two-rowed barley, 78 %, whereas six-rowed barley was only 22 %. The spike colours present were light yellow, dark yellow, brown and violet. The material varied also for such characters as length of spike and number of kernels per spike. For the SSR loci 129 alleles were detected with an average of 9.3 alleles per locus and a genetic diversity with an average of h = 0.721 per locus. Based on analysis of molecular variance the 71.3 % of genetic diversity accounted for within accession variation, whereas 27.4 % for among accession variation. The variation between provinces was not significantly different. A group of two-rowed barley was identified that was more closely related to six-rowed barley than to the rest of the two-rowed barley material. Two-rowed material currently grown in Kyrgyzstan is more diverse than six-rowed barley (h = 0.65, A = 8.8 vs. h = 0.59, A = 3.1). From this study we could conclude that the materials nowadays grown by farmers are mixtures. Since there are no geographic differentiation between these mixtures suggests that materials is frequently inter-exchanged between farmers and are most probably not landrace material. The question concerning the origin of these mixtures remains unsolved and further investigation will be carried out to identify sources of variation in farmers’ field.     

Effect of Soil Sample Density on Nitrogen Application Maps and Sugar Beet Root Yield and Quality

Abstract

Production of high‐quality sugar beets requires precise nitrogen (N) fertilizer management with N recommendations based on a measurement of residual nitrate‐N to 120 cm (4 ft) soil depth. The objective of this study was to determine the agronomic and economic aspects of using different grid cell sizes for sampling soil nitrate‐N. A 5‐ha field (12.4 acre) was soil sampled for nitrate‐N using 18.3×18.3 m grid cells in 1997 and 1998. Nitrogen application maps for four different grid cell sizes 18.3×18.3 m (60×60 ft), 54.9×54.9 m (180×180 ft), 91.4×91.4 m (300×300 ft), and 128×128 m (420×420 ft) were developed from these soil samples and compared with a conventional average for each field. The five nitrogen fertilizer treatments adjusted for grid cell size were applied with a fertilizer truck equipped with a variable rate applicator in two replicates of 274‐m long strips. Sugar beet quality was determined on root samples taken immediately before root harvest on a 18.3×18.3 grid. Root yield was determined with a conventional harvester equipped with a yield monitor. The nitrogen fertilizer application maps derived from the 54.9×54.9 m, 91.4×91.4 m, and 128×128 m soil sampling grids were similar in 1997. The application map derived from 18.3×18.3 m grid cell size was different from the application maps derived from larger grid cell sizes. Although there were differences in N application maps, there were no differences in root yield, root quality, or recoverable sucrose among grid cell sizes or constant rate applications in either 1997 or 1998.     

Influence of Nitrogen and Potassium Fertilization on Yield and Quality of Rosemary in Relation to Harvest Number

Rosemary is an important aromatic and spice plant. Its cultivation for production of its essential oil has gained importance in recent years. An experiment was conducted in a red sandy loam (Kandiustalf) soil to study the impact of cultivation of this multiharvest crop at different nitrogen (N) and potassium (K) application regimes on soil fertility and crop yield at the farm of the Central Institute of Medicinal and Aromatic Plants, Resource Center, Bangalore, during 2003–2005. The treatments consisted of all combinations of N levels (0, 150, and 300 kg per hectare per year) and K levels (0, 50, and 100 kg per hectare per year). Five harvests were taken at about 5‐month intervals. The results showed that initially the soil alone was able to meet the crop requirements for K, but with the progress of time, addition of K to the soil in the form of fertilizer became necessary for obtaining optimum yields. At the end of five harvests, exchangeable K in soil was significantly lower as application of K decreased from 100 to 50 and 0 kg per hectare per year. Soil fertility could be maintained and oil yields of rosemary from four harvests could be increased from 146.6 L to 344.7 L per hectare by application of 150 kg N and 100 kg K.     

Planting Date and Variety Effects on Rice Main and Ratoon Crop Production in South Texas

Rice ratooning could be a practical way to increase rice production per unit area and per unit time. The objectives of this study were to evaluate variety [two inbreds (Cocodrie and Presidio) and three hybrids (Clearfield XL729, Clearfield XL745, and XL723)] and planting date (early and normal) effects on rice ratoon production in a sandy loam and clay soil at Eagle Lake and Beaumont, Texas, USA, respectively. A randomized design with four replications was used at both sites in 2008 and 2009 with a total of 160 observations. A PROC MIXED model was used to analyze the data with year and site as random factors and planting date and variety as fixed factors, and main crop (MC) and ratoon crop (RC) were analyzed using Repeated Measures analysis of variance (ANOVA). The grain yield of MC was only affected by the variety with hybrids having greater yields than inbreds (p < 0.01). However, both RC and total (MC plus RC) grain yields were affected by both planting date and variety (p < 0.01). For both RC and TC crops, early planting had greater (10%) grain yields than the normal planting (p < 0.01). Hybrids had 12% greater rain yields than inbreds (p < 0.001), but only for RC. The rice milling quality of MC was also affected by planting date and variety with greater milling yields with inbreds under normal planting. The results from this study indicate that rice ratoon and total crop yields could be improved through a combination of selected management practices including optimal variety selection and an earlier planting date.     

Organic carbon fractions and micronutrient status of soils under some plantation crops in southwestern Nigeria

The organic carbon (OC) fractions and micronutrient status of soil under cashew (Anacardium occidentale), plantain (Musa spp), oil-palm (Elaeis guineensis) and gmelina (Gmelina arborea) plantations were studied to determine their effectiveness for managing converted forest in the derived savanna ecology of southwestern Nigeria. The control secondary forest soil had statistically similar OC (2.11%) at 0–15 cm with gmelina but significantly higher than those of the remaining plantation crops. At deeper depths, however, the OC content of the control forest was lower than those of other plantation crops. Apart from gmelina, other plantation crops had carbon enrichment ratio (CER) <1 at 0–15 cm. Only gmelina sequestered as much non-hydrolysable carbon (NHC) as the control at 0–15 cm depth. Also it was observed that plantain plantation sequestered higher NHC than the control at 15–30 cm depth. At >30 cm depth, however, none of the plantation crops sequestered as much NHC as the control. Using the average NHC at all the soil depth as a quality index, gmelina has a better potential to sequester NHC than the other plantation crops used in this study.     

The Impact of Irrigated Agriculture on Water Quality of Rivers Kongoni and Sirimon, Ewaso Ng’iro North Basin, Kenya

This study aimed at determining the water quality of River Sirimon and River Kongoni, Ewaso Ng'iro North Basin, Kenya. Water quality analysis of these two rivers was done for a period of 5 months between November 2005 and February 2006. Portable Palintest equipment was used for the chemical analysis. The study established that there were sulphates concentrations of 22 mgL^?1 in the Kongoni River water associated with the use of commercial fertilisers as compared to mean values of 7 mgL^?1 along Sirimon River; phosphate concentrations were 1.3 mgL^?1 in Kongoni and 0.15 mgL^?1 in Sirimon and salinity 3 mgL^?1 in Kongoni and 0.47 mgL^?1 in Sirimon. On average, mean nitrates concentrations of 1.7 mgL^?1 were recorded for Kongoni River, which were higher than those recorded for Sirimon River (0.033 mgL^?1). These concentration levels were however within the standard levels set by WHO for example 50 mgL^?1 for nitrates (WHO 2008). River Kongoni has two major irrigated horticultural farms across it which were likely polluting the river during the time of this study.     

Agronomic performance and grain quality of sesame (<Emphasis Type="Italic">Sesamum indicum</Emphasis> L.) landraces and improved varieties grown in a Mediterranean environment

Sesame seeds are an excellent food and non-food raw material, for which there is a consolidated deficit in Italy and in other European Union countries. For this reason, a 2-years experiment was conducted in Sicily to compare the agronomic performance (phenology, morphological and productive traits) and grain quality (oil and its main constituents, protein of defatted flour, fibre) of three landraces, one of Turkish origin and two Sicilian (“Ispica” and “Modica”), and two improved varieties (‘Pachequino’ and ‘Yori 77’). The landraces evidenced earliness (115 days) and the greater height of insertion of first capsule (0.52 m), whereas the variety ‘Pachequino’ was the most productive (3.5 t ha^?1). Turkish and “Ispica” landraces and ‘Yori 77’ variety provided seeds with greater lipid (54 %, on average) and protein contents (44 % on defatted flour, on average). ‘Pachequino’ and both Sicilian landraces produced seeds richer in fibre fractions. As regard to oil quality, the oleic acid/linoleic acid was found balanced (about 1) for Turkish landrace, and it decreased for the other cultivars reaching the lowest value for ‘Pachequino’. “Modica” had higher quantity of unsaponifiable matter (1.98 %) in the oil, whereas ‘Yori 77’ had the maximum concentration of phytosterols (5532.8 mg kg^?1). Policosanol fraction prevailed in oil of “Ispica” (205.8 mg kg^?1). Moreover, there was variability in the fatty acid, sterol and policosanol compositions with differences among the cultivars. Research provide information to exploit sesame within agrosystems under Mediterranean climates, and may be a starting point for breeding activity to enhance crop productivity and grain quality.     

Effects of vermicompost and vermiwash biofertilizers on fenugreek (Trigonella foenum) plant

ABSTRACT

In order to evaluate the effect of vermicompost and vermiwash biofertilizers on the traits of fenugreek (Trigonella foenum) plant, a field experiment of a randomized complete block design with 8 treatments and 3 replications was conducted. The treatments consisted of control without fertilizer, vermicompost at 5, 10, and 15 tons per hectare, vermiwash spraying with 3,000, 6,000, and 12,000 ppm, and a combined application of 10 tons of vermicompost with 6,000 ppm vermiwash. In this research, traits of crop growth rate (CGR), pod length, pod width, grain yield, chlorophyll a and b, essential oil percentage, and essential oil yield were evaluated. The results revealed that the use of vermicompost biofertilizer increased the measured quantitative traits more than that of the vermiwash. The most and the least effects on all traits, except for the length of the pod, were 15 and 5 tons vermicompost per hectare, respectively. The treatment of 10 tons of vermicompost with 6,000 ppm vermiwash did not show significant differences in 10 and 15 tons vermicompost per hectare in most traits. It was observed that the application of vermicompost fertilizer leads to weaker results than the vermiwash fertilizer. With the increase of vermiwash concentration, the effect of this fertilizer on the measured traits reduced; however, in most traits, the results of the application of 3,000 and 6,000 ppm vermiwash had a statistically significant effect on the treatment with 5 tons of vermicompost per hectare. Overall, this experiment showed that all the evaluated traits in this study have a positive reaction to the application of vermicompost and vermiwash biofertilizers. Also, the response of the fenugreek plant to the vermicompost is much greater than the vermiwash fertilizer.