Transport Of Fecal Bacteria From Poultry Litter and Cattle Manures Applied to Pastureland

Land applications of manure from confined animal systems and direct deposit by grazing animals are both major sources of bacteria in streams. An understanding of the overland transport mechanisms from land applied waste is needed to improve design of best management practices (BMPs) and modeling of nonpoint source (NPS) pollution. Plots were established on pasturelands receiving phosphorus-based livestock waste applications to measure the concentrations of Escherichia coli (E. coli), fecal coliform (FC), and Enterococcus present in overland flow at the edge of the field. The flow-weighted bacteria concentrations were highest in runoff samples from the plots treated with cowpies (1.37×10⁵ colony forming units (cfu)/100 ml of E. coli) followed by liquid dairy manure (1.84×10⁴ cfu/100 ml of E. coli) and turkey litter (1.29×10⁴ cfu/100 ml of E. coli). The temporal distribution of fecal bacterial concentrations appeared to be dependent upon both the animal waste treatment and the indicator species, with peak concentrations occurring either at the beginning of the runoff event or during peak flow rates. BMPs could be selected to reduce peak flows or first flush effects depending upon the litter or manure applied to the land. The commercial Biolog System was used to identify the dominant species of Enterococcus present in the cowpie source manure (Enterococcus mundtii 55%) and in the runoff collected from the transport plots treated with cowpies (Enterococcus faecalis 37%). The identification of predominant species of Enterococcus that are associated with specific sources of fecal pollution could greatly assist with identifying the origins of NPS pollution.     

Preferential Attachment of Escherichia coli to Different Particle Size Fractions of an Agricultural Grassland Soil

This study reports on the attachment preference of a faecally derived bacterium, Escherichia coli, to soil particles of defined size fractions. In a batch sorption experiment using a clay loam soil it was found that 35% of introduced E. coli cells were associated with soil particulates >2 μm diameter. Of this 35%, most of the E. coli (14%) were found to be associated with the size fraction 15–4 μm. This was attributed to the larger number of particles within this size range and its consequently greater surface area available for attachment. When results were normalised with respect to estimates of the surface area available for bacterial cell attachment to each size fraction, it was found that E. coli preferentially attached to those soil particles within the size range 30–16 μm. For soil particles >2 μm, E. coli showed at least 3.9 times more preference to associate with the 30–16 μm than any other fraction. We report that E. coli can associate with different soil particle size fractions in varying proportions and that this is likely to impact on the hydrological transfer of cells through soil and have clear implications for our wider understanding of the attachment dynamics of faecally derived bacteria in soils of different compositions.     

Runoff and Sediment Attenuation by Undisturbed and Lightly Disturbed Forest Buffers

A runoff plot experiment found that ten metre undisturbed forest buffers removed80–90% of runoff and over 95% of sediment produced by logging skid tracks. The study was carried out on 21^º slopes in a native forest in eastern New South Wales, Australia. The experiment included three replicates of four treatments including undisturbed control, skid track, skid track + undisturbed buffer and skid track + disturbed buffer. Skid track and control plots were 20 m long by 5 m wide. Buffer plots consisted of a 20 m by 5 m skid track directing runoff to a 10 m by 5 m naturally vegetated buffer that was either undisturbed or lightly disturbed. Runoff and sediment yields from plots were monitored over two successive summers. Undisturbed buffers greatly reduced overland flow and decreased sediment yields from around 100 Mg ha¹ to less than 0.5 Mg ha^-1. Differences in both runoff and sediment yield between undisturbed buffer and control treatments were minimal and not statistically significant. Disturbed buffers achieved similarly large reductions in runoff and sediment yield in two out of three replicates. The third replicate yielded as much or more runoff and sediment than the skid track plots suggesting that disturbance increased the risk of buffer failure. The peak rate of outflow from buffer plots was generally not related to peak buffer inflow until a threshold inflow of 1.6 L s^-1 was reached, after which peak outflow and peak inflow were linearly related.     

Manure as a Source of Antibiotic-Resistant Escherichia coli and Enterococci: a Case Study of a Wisconsin, USA Family Dairy Farm

The spread of antibiotic-resistant bacteria in the environment is raising serious public health concerns, and manure is being increasingly recognized as a major source of antibiotic-resistant bacteria. In this research, we isolated Escherichia coli and enterococci from manure produced in a Wisconsin, USA family dairy farm to determine their resistance to six representative antibiotics. The average densities for E. coli and enterococci were 6.37(±4.38)?×?10⁷ colony formation units (CFU)?g^?1 and 1.60(±1.57)?×?10⁴ CFU g^?1, respectively. The E. coli isolates were found to be resistant to cephalothin, ampicillin, tetracycline, and erythromycin. In addition to these four antibiotics, the Enterococcus isolates were also resistant to gentamicin and ciprofloxacin. Additionally, we examined the survival and growth of E. coli and enterococci in dairy manure over a period of ~3 days. While the densities of enterococci remained stable over the study period, the concentrations of E. coli on average increased by 1.5 log10 units. Further tests of the bacterial antibiotic resistance over time showed no significant changes in the prevalence of antibiotic resistance. This result indicated that slightly aged manure could represent a larger source of antibiotic-resistant E. coli than fresh manure and the accumulation of antibiotic-resistant E. coli and enterococci in the agricultural fields must be accounted for in the modeling of the spread of antibiotic-resistant bacteria in the environment.     

Relationship Between Fecal Indicators in Sediment and Recreational Waters in a Danish Estuary

The European Union has introduced a new bathing water directive where future classification of recreational waters will be based on the microbial parameters Escherichia coli, and intestinal enterococci. Introduction of enterococci as a new quality parameter may pose a challenge in some areas because relatively less is known about these organisms compared to E. coli. In the present study, the relative abundance of intestinal enterococci, E. coli, and ten fecal sterol and stanol biomarkers were investigated in water and sediment at two estuarine beach sites affected by fecal pollution. In the bathing water, enterococci were relatively more abundant at low E. coli concentrations. In the sediment, enterococci were generally more abundant than E. coli with surface concentrations between 1.0?×?10² and 4.5?×?10³ CFU cm^?3. Enterococci populations were relatively similar in water and sediment, and were phenotypically different from that of nearby pollution sources. The putative human specific genetic marker esp in Enterococcus faecium was not detected in water or sediment samples despite occasional inputs of human waste from storm water overflows. Sterol and stanol profiles suggested a direct link between water and sediment pollution profiles on days with wind conditions that facilitated resuspension. Sediment resuspension may occur at wind speeds exceeding 6–8 m s^?1, and could contribute significantly to enterococci concentrations in the overlying water. The study emphasized that recontamination of the water column due to wind induced resuspension should be considered when evaluating indicator levels and microbial hazards in estuarine recreational waters.     

Nutrient Movement Due to Overland Flow in Managed Native Eucalyptus Forests,Southeastern Australia

A rainfall simulator study of total nutrient movement due to overland flow on forest skid trails and general harvesting areas (GHA) in the native eucalypt forests of southeastern Australia, confirmed that the disturbed and compacted areas were the dominant sources of sediment-bound pollutants. Low runoff production and low sediment availability on the rough surfaces of the GHA reduced total nutrient yields. On the more recently disturbed and burnt sites, the solution pathway may have been more dominant due to runoff coming in contact with surface ash and charcoal. We also recorded significant reductions in the total nutrient yields as runoff from the skid trail was delivered to the adjacent hillslope via a cross bank (water bar). This was due primarily to coarse-grained sediment deposition at the base of the banks. Nutrient concentrations in the eroded sediment at the cross bank outlets, however, remained 9 times higher than the in-situ surface soils, due to the preferential delivery of fine aggregate material to the adjacent hillslope. Further reductions in total nutrient yields occurred as overland flow passed through the GHA, illustrating the effectiveness of vegetative filtering in reducing runoff volumes and sediment fluxes. Temporal trends in the data highlighted significant reductions in total nutrient movement on both areas of disturbance as sites regenerated over a period of ～ 5 years. Water pollution abatement practices in these environments should aim to locate skid trails to maximise the redistribution of runoff and sediment from highly disturbed areas to more vegetated parts of the hillslope, and by appropriate cross bank spacing, reduce the volume of overland flow carrying sediment and nutrients.     

Concentrations and Exports of Fecal Indicator Bacteria in Watersheds with Varying Densities of Onsite Wastewater Systems

The research goal was to determine if onsite wastewater system (OWS) density had an influence on the concentrations and watershed exports of Escherichia coli and enterococci in urbanizing watersheds. Eight watersheds with OWS densities ranging from <?0.1 to 1.88 systems ha^?1 plus a watershed served by sewer (Sewer) and a mostly forested, natural watershed (Natural) in the Piedmont of North Carolina served as the study locations. Stream samples were collected approximately monthly during baseflow conditions between January 2015 and December 2016 (n?=?21). Median concentrations of E. coli (2014 most probable number (MPN) 100 mL^?1) and enterococci (168 MPN 100 mL^?1) were elevated in streams draining watersheds with a high density of OWS (>?0.77 system ha^?1) relative to watersheds with a low (<?0.77 system ha^?1) density (E. coli: 204 MPN 100 mL^?1 and enterococci: 88 MPN 100 mL^?1) and control watersheds (Natural: E. coli: 355 MPN 100 mL^?1 and enterococci: 62 MPN 100 mL^?1; Sewer: 177 MPN 100 mL^?1 and 130 MPN 100 mL^?1). Samples collected from watersheds with a high density of OWS had E. coli and enterococci concentrations that exceeded recommended thresholds 88 and 57% of times sampled, respectively. Results show that stream E. coli and enterococci concentrations and exports are influenced by the density of OWS in urbanizing watersheds. Cost share programs to help finance OWS repairs and maintenance are suggested to help improve water quality in watersheds with OWS.     

Soil erosion and runoff in different vegetation patches from semiarid Central Mexico

Vegetation patches in arid and semiarid areas are important in the regulation of surface hydrological processes. Canopy and ground covers developed in these fertility islands are a natural cushion against the impact energy of rainfall. Also, greater levels of organic matter improve the soil physicochemical properties, promoting infiltration and reducing runoff and soil erosion in comparison with the open spaces between them. During the 2006 rainy season, four USLE-type plots were installed around representative vegetation patches with predominant individual species of Huisache (Acacia sp), Mesquite (Prosopis sp), Prickly Pear or Nopal (Opuntia sp) and Cardon (Opuntia imbricata), to evaluate soil erosion and runoff, in semiarid Central Mexico. A comparative bare surface condition (Control) was also evaluated. Vegetative canopy and ground cover were computed using digital images. Selected soil parameters were determined. Soil erosion was different for the studied vegetation conditions, decreasing as canopy and ground cover increased. There were not significant differences in runoff and soil erosion between the Control and O.imbricata surfaces. Runoff was reduced by 87%, 87% and 98% and soil loss by 97%, 93%, and 99% for Acacia farnesiana, Prosopis laevigata and Opuntia sp, respectively, as compared to the Control. Soil surface physical conditions were different between the low vegetation cover conditions (Control and O.imbricata surfaces) and the greater vegetation cover conditions (A.farnesiana, P.laevigata and Opuntia sp), indicating a positive effect of vegetation patches on the regulation of surface hydrological processes.     

Seasonal variability of runoff and soil loss on forest road backslopes under simulated rainfall

Runoff and soil loss from forest road backslopes is a serious problem in Mediterranean areas. Surface runoff and sediment production on backslopes of forest roads in Los Alcornocales Natural Park (southern Spain) has been studied in this paper using a simple portable rainfall simulator at an intensity of 90 mm h^− 1. One hundred rainfall simulations were performed on bare and vegetated road backslopes during summer and winter in order to study seasonal differences. Runoff coefficients and soil loss rates were lower on the vegetated plots than on the bare ones. Runoff coefficients increased 1.7 (bare backslopes) and 3.1 times (vegetated backslopes) from summer to winter. Preserving the vegetation cover over 20% is recommended for keeping soil loss rates under low levels, especially during winter.     

Effects of 30-year-old Aleppo pine plantations on runoff,soil erosion,and plant diversity in a semi-arid landscape in south eastern Spain

Forest management policies in Mediterranean areas have traditionally encouraged land cover changes, with the establishment of tree cover (Aleppo pine) in natural or degraded ecosystems for soil conservation purposes: to reduce soil erosion and to increase the vegetation structure. In order to evaluate the usefulness of these management policies on reduced erosion in semi-arid landscapes, we compared 5 vegetation cover types (bare soil, dry grassland, shrublands, afforested dry grasslands and afforested thorn shrublands), monitored in 15 hydrological plots (8 × 2 m), in the Ventós catchment (Alicante, SE Spain), over 4 years (1996 to 1999). Each cover type represented a different dominant patch of the vegetation mosaic on the north-facing slopes of this catchment. The results showed that runoff coefficients of vegetated plots were less than 1% of the precipitation volume; whereas runoff in denuded areas was nearly 4%. Soil losses in vegetation plots averaged 0.04 Mg ha^− 1 year^− 1 and increased 40-fold in open-land plots. The evaluation of these forest management policies, in contrast with the natural vegetation communities, suggests that: (1) thorn shrublands and dry grassland communities with vegetation cover could control runoff and sediment yield as effectively as Aleppo pine afforestation in these communities, and (2) afforestation with a pine stratum improved the stand's vertical structure resulting in pluri-stratified communities, but reduced the species richness and plant diversity in the understorey of the plantations.     

Survival of <Emphasis Type="Italic">Escherichia coli</Emphasis> O157:H7 in various soil particles: importance of the attached bacterial phenotype

The risk of enteropathogens to food and water is highly dependent on their survival in soil environments. Here, the effects of soil type, particle size, the presence of natural organic matter (NOM) or Fe/Al (hydro)oxides on pathogenic Escherichia coli O157:H7 survival in sterilized soil particles were assessed through survival, attachment, metabolic activity, and qRT-PCR analyses. The abundance of inoculated E. coli O157:H7 in Brown soil (Alfisol) particles increased 0.6–1.4 log₁₀ CFU/g within 3 days (except for NOM-stripped clay), while that in Red soil (Ultisol) particles decreased rapidly in 8 days post-inoculation. Additionally, survival of bacteria was significantly enhanced when Fe/Al (hydro)oxides had been removed from Red soil particles. For the two soils, E. coli O157:H7 survived the longest in NOM-present clays and the bacterial adenosine 5′-triphosphate (ATP) levels were 0.7–2.0 times greater in clays than in sands and silts on day 8. Moreover, clays were more effective than silts and sands in binding cells and changing the expressions of acetate pathway-associated genes (pta and ackA). For silts and sands, E. coli O157:H7 decayed more rapidly in the presence of NOM and similar trends of bacterial ATP levels were observed between NOM-stripped and NOM-present soil particles, indicating that the primary role of NOM was not as a nutrient supply. These findings indicate that soil particles function mainly through attachment to change the metabolic pathway of E. coli O157:H7 and ultimately impact the survival of bacterial pathogens in soils.     

Relative risk of surface water pollution by E. coli derived from faeces of grazing animals compared to slurry application

Abstract. This article examines some of the factors that influence the relative risk of Escherichia coli pollution of surface waters from grazing animals compared to cattle slurry application. Drainage water from pipe‐drained plots grazed with sheep (16 sheep + lambs per hectare) from 29 May to 17 July 2002 had average E. coli counts of 11 c.f.u. mL^?1 or 0.4% of estimated E. coli inputs over the grazing period. Drainage water from plots on the same site treated with cattle slurry (36 m³ha^?1 on 29 May 2002) had lower average E. coli counts of 5 c.f.u. mL^?1 or 0.03% of estimated faecal input. Sheep (16 lambs per hectare) grazing under cooler, moister conditions from 24 September to 3 December 2001 gave drainage water with much higher average E. coli counts of 282 c.f.u. mL^?1 or 8.2% of estimated input, which is more than twice the average E. coli counts previously reported under such conditions (Vinten et al. 2002 Soil Use and Management 18, 1–9). Laboratory studies of runoff from soil slabs after slurry application showed that the mobility of E. coli in surface soil decreased with time, suggesting that increased attachment to soil or migration to ‘immobile’ water also provides at least part of the physical explanation for the relatively higher risk of pollution from grazing animals compared with slurry. Sampling for E. coli in field drainflow and in streamwater during a storm event in the predominantly dairy Cessnock Water catchment, Ayrshire, Scotland supported the hypothesis that E. coli transport is linked to grazing animals. For a 7‐mm rainfall event, roughly 14% of the estimated daily input from grazing livestock was transported to the river, even though little slurry spreading had occurred in the catchment in the previous month. Spot sampling of field drains in grazed fields and silage fields in the same catchment also showed that grazing animals were the principal source of E. coli and faecal streptococci.     

Comparative diversity and abundance of ammonia monooxygenase genes in mulched and vegetated soils

Soil microbial habitats are altered by mulching, a common practice in urban areas during which vegetation is removed and soils covered to suppress weeds and retain moisture. Soil microorganisms drive nitrogen-cycling processes in mulched soils, because living plants no longer take up ammonium-N released during decomposition of residual organic matter. Because ammonia oxidizers carry out the first, rate-limiting step of nitrification, we compared ammonia oxidizers in experimental, unfertilized plots of mulched and vegetated soils. We hypothesized that mulched and vegetated soils would support contrasting communities of bacterial and archaeal ammonia oxidizers, as determined by quantitative PCR and primers specific for genes encoding ammonia monooxygenase subunit A (amoA). Clone libraries of archaeal amoA also were constructed to compare diversity in soil cores, duplicate blocked plots, and treatments (bark-mulched, gravel-mulched, and unmanaged old field vegetation). Gene copies from ammonia-oxidizing bacteria (AOB) ranged from 2.2 × 10⁶ to 2.7 × 10⁷ gene copies per gram dry soil and did not differ across treatments. In contrast, gene copies from ammonia-oxidizing archaea (AOA) ranged from 9.1 × 10⁵ to 1.0 × 10⁸ copies per gram dry soil, with bark-mulched soils having significantly lower abundance. Community structure of AOA in gravel-mulched soils was distinct from the other two treatments. At 97% amino acid similarity, 22 operational taxonomic units, or OTUs, were identified, with only one OTU found in all 18 clone libraries. This ubiquitous OTU-1, which was highly similar to published amoA sequences recovered from soils, comprised 55% of all 482 translated sequences. Greater variability in OTU richness was observed among cores from mulched soils than from vegetated soils. Our observations supported our hypothesis that AOA communities differ in mulched and vegetated soils, with mulched soils providing altered and variable microniches for these N cycling microorganisms.     

Hydrological pathways of metolachlor export from an agricultural watershed

Hydrological pathways of metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl) acetamide] transport from a 5.5 ha^?1 corn field in southern Ontario were examined. Metolachlor was applied as an early post-emergence surface broadcast treatment at a rate of 334.1 mg active ingredient m^?2. Persistence in soils decreased exponentially following application, with slower dissipation in footslope areas. Metolachlor concentrations in runoff from experimental plots also decreased exponentially with time, and were strongly associated with herbicide levels in surrounding soils. Differences between concentrations and yields in saturation overland flow and Horton overland flow during the monitoring period were controlled by near-stream water table conditions. Total herbicide losses in runoff from the plots were in the order of 1% of applied a.i. Metolachlor was rapidly leached to depths of 1 m, such that peak concentrations and instantaneous metolachlor loads in tile drainage were observed within 14 days following application. Peak concentration in streamflow (125 µg L-1) occurred during the first major runoff event 13 days following application; peak concentrations in succeeding events followed an exponential decay during the growing season. Export of metolachlor from the field was in the order of 1% of applied a.i., with overland flow serving as the dominant transport mechanism from site of application. Near-stream hydrologic conditions may determine whether field exports of herbicide enter receiving water courses.     

Effect of ground‐cover type on surface runoff and subsequent soil erosion in Champagne vineyards in France

This study was conducted in Champagne vineyards in France, and the objectives were to compare the main cultivation practices in Champagne vineyards and to specify the conditions required for the optimum effect of inter‐row grass cover on runoff and erosion in experimental plots of 0.25 m² under simulated rainfall. Three types of ground cover were studied. In the bark‐and‐vine‐prunings plots, the runoff coefficient (RC) ranged from 1.3 to 4.0% and soil losses were <1 g/m²/h. In the bare soil (BS) plot, the highest RC of the study was found (80.0%) and soil losses reached 7.4 g/m²/h. In the grass cover plots, the RC and amount of eroded soil were highly variable: the RCs ranged from 0.4 to 77.0%, and soil losses were between less than 1 and 13.4 g/m²/h. Soil type, soil moisture, slope and agricultural practices did not account for the variability. In fact, the density of grass cover in the wheel tracks explained a portion of this variability. The lack of grass in the centre of the inter‐row allowed for a preferential flow and created an erosion line in the wheel tracks where the soil was compacted. This study showed that grass cover in a vineyard was not necessarily sufficient to reduce surface runoff and prevent soil erosion. To be effective, the grass cover must be dense enough in the wheel tracks of agricultural machinery to avoid RCs close to the RC achieved with BS.     

坡地开垦的径流泥沙响应

Land use and land cover change is a key driver of environmental change. To investigate the runoff and erosion responses to frequent land use change on the steep lands in the Three Gorges area, China, a rainfall simulation experiment was conducted in plots randomly selected at a Sloping Land Conversion Program site with three soil surface conditions: existing vegetation cover, vegetation removal, and freshly hoed. Simulated rainfall was applied at intensities of 60 (low), 90 (medium), and 120 mm h 1 (high) in each plot. The results indicated that vegetation removal and hoeing significantly changed runoff generation. The proportion of subsurface runoff in the total runoff decreased from 30.3% to 6.2% after vegetation removal. In the hoed plots, the subsurface runoff comprised 29.1% of the total runoff under low-intensity rainfall simulation and the proportion rapidly decreased with increasing rainfall intensity. Vegetation removal and tillage also significantly increased soil erosion. The average soil erosion rates from the vegetation removal and hoed plots were 3.0 and 10.2 times larger than that in the existing vegetation cover plots, respectively. These identified that both the runoff generation mechanism and soil erosion changed as a consequence of altering land use on steep lands. Thus, conservation practices with maximum vegetation cover and minimum tillage should be used to reduce surface runoff and soil erosion on steep lands.     

Slope sediment yield in arid lowland continuous permafrost environments, Canadian Arctic Archipelago

Surface wash erosion was measured at runoff plots on low to moderate slopes in clayey and sandy silts underlain by continuous permafrost on the Fosheim Peninsula, Ellesmere Island. Due to snow redistribution in winter, total precipitation on the plots varied from 34 to 150 mm, with corresponding surface runoff values of 0 to 102 mm. Where runoff occurred, at least 80% of it was derived from snowmelt. Suspended sediment removal was <75 g m⁻² a⁻¹ at relatively well-vegetated sites but averaged more than 1200 g m⁻² a⁻¹ at a plot where the vegetation had been removed by landsliding. Niveo-aeolian deposition was greater than suspended sediment removal at some plots, indicating net accumulation. Solute removal ranged up to 80 g m⁻² a⁻¹ and exceeded clastic sediment transport at one vegetated site. Elevated rates of erosion at the sites of detachment slides that pre-date 1950 demonstrated that terrain disturbance in permafrost environments can affect slopewash processes for at least several decades.Plot data (precipitation, vegetation and surface grain-size) from the Fosheim Peninsula and Banks Island were used to develop a statistical model of suspended sediment removal by surface wash on undisturbed slopes. For any given grain-size, the model predicts a rise in erosion from zero precipitation (because of an absence of runoff) to a peak at about 50 mm, a decline as precipitation increases to 300 mm and a further increase in erosion beyond this inflection point. This non-linear response is due to the complex interaction of moisture (primarily snow) and vegetation cover. Erosion at any given precipitation value varies through three orders of magnitude depending on surface grain-size. The maximum erosion predicted is 1 kg m⁻² a⁻¹ for a runoff plot with 1100 mm of precipitation, a corresponding vegetation cover of 77% and a median surface grain-size of 7 φ.     

Investigation of environmental and land use impacts in forested permafrost headwaters of the Selenga-Baikal river system,Mongolia - Effects on discharge,water quality and macroinvertebrate diversity

Headwater streams play a major role for provision of ecosystem services, e.g. drinking water. We investigated a high-altitude headwater catchment of the Kharaa River (including 41 1st-order rivers) to understand the impact of land cover (especially forest cover), environment and human usage on runoff, chemical water quality and macroinvertebrate fauna in a river basin under discontinuous permafrost conditions in an arid, sparsely populated region of Mongolia. To verify our hypotheses that different landuses and environmental impacts in permafrost headwaters influence water quality, we investigated 105 sampling sites, 37 of them at intermittent stream sections without water flow. Discharge was positively impacted by land cover types steppe, grassland and forest and negatively by shrubland, forest burnt by wild fires (indicating a reduction of permafrost) and slope. Water quality was affected by altitude, longitude and latitude, shrub growth and water temperature. Shannon diversity of macroinvertebrates was driven by water temperature, iron content of the water, flow velocity, and subbasin size (adjusted R² = 0.54). Sample plots clustered in three groups that differed in water chemistry, macroinvertebrate diversity, species composition and bio-indicators. Our study confirms that steppes and grasslands have a higher contribution to runoff than forests, forest cover has a positive impact on water quality, and diversity of macroinvertebrates is higher in sites with less nutrients and pollutants. The excellent ecological status of the upper reaches of the Kharaa is severely threatened by forest fires and human-induced climate change and urgently needs to be conserved.     

Effectiveness of geotextiles in reducing runoff and soil loss: A synthesis

Despite geotextiles having potential for soil conservation, limited scientific data are available to assess the effects of geotextiles in reducing runoff and water erosion. Hence, the objective of this review is to analyse the effects of plot length (L) and other possible affecting factors [cover percentage (C, %), slope gradient (S), rainfall duration (D), rainfall intensity (I), sand, silt and clay contents, soil organic matter (SOM) content and geotextile type (natural or synthetic)] on the effectiveness of geotextiles in reducing soil and water loss, based on reported experimental data. From linear regressions, C (%) and soil sand, silt and clay contents are found to be the most important variables in reducing SLR (ratio of soil loss in bare plots to that in geotextile treated plots) for splash, C (%) for interrill and D (min) for rill and interrill erosion processes, respectively. Soil clay and silt contents and D are key variables in decreasing RR (ratio of runoff from bare plots to that from geotextile treated plots) for interrill, and clay content for rill and interrill erosion processes, respectively. The linear relationship between mean b-value (geotextile effectiveness factor in reducing soil loss) and L of all studies was not significant (P > 0.05). The same is true for the relationship between L and SLR, and L and RR. However, when L is added to an equation as an interaction term with C (%), geotextile cover is significantly (P < 0.05) more effective in reducing SLR on shorter plots than longer ones for both interrill and rill and interrill erosion processes. Buffer strip plots (area coverage ∼ 10%) with Borassus and Buriti mats have the highest b-values.