为探索负压供水对辣椒土壤速效养分和土壤生物活性的影响,在温室大棚内采用盆栽试验,试验利用负压供水装置,设置-5、-10、-15 k Pa以及人工浇水(CK)4个处理,测定了辣椒各生育期土壤碱解氮、有效磷、速效钾含量,以及土壤过氧化氢酶、脲酶、蔗糖酶和磷酸酶活性指标。结果表明,试验中供水压力控制在-5~-15 k Pa时,辣椒各生育期土壤碱解氮、有效磷、速效钾含量,4种土壤酶活性均显著高于CK,其中,-5k Pa处理对土壤养分及酶活性的提高显著。土壤速效养分含量与酶活性相关分析表明,在负压供水条件下,土壤4种酶活性之间,土壤碱解氮、有效磷、速效钾含量之间,土壤酶与土壤速效养分之间均呈极显著正相关;主成分分析表明,第一主成分对土壤养分供应能力的贡献大,第二主成分反映出过氧化氢酶及蔗糖酶不断分解减少时,有效磷及速效钾权重减少。综上所述,采用负压供水方式,能促进土壤酶活性提高,有利于辣椒土壤养分的转化,当供水压力稳定在-5 k Pa时,提高辣椒土壤养分效果更为显著。 相似文献
Microbial activity is affected by changes in the availability of soil moisture. We examined the relationship between microbial activity and water potential in a silt loam soil during four successive drying and rewetting cycles. Microbial activity was inferred from the rate of CO2 accumulating in a sealed flask containing the soil sample and the CO2 respired was measured using gas chromatography. Thermocouple hygrometry was used to monitor the water potential by burying a thermocouple in the soil sample in the flask. Initial treatment by drying on pressure plates brought samples of the test soil to six different water potentials in the range -0.005 to -1.5MPa. Water potential and soil respiration were simultaneously measured while these six soil samples slowly dried by evaporation and were remoistened four times. The results were consistent with a log-linear relationship between water potential and microbial activity as long as activity was not limited by substrate availability. This relationship appeared to hold for the range of water potentials from ?0.01 to ?8.5 MPa. Even at ?0.01 MPa (wet soil) a decrease in water potential from ?0.01 to ?0.02 MPa caused a 10% decrease in microbial activity. Rewetting the soil caused a large and rapid increase in the respiration rate. There was up to a 40-fold increase in microbial activity for a short period when the change in water potential following rewetting was greater than 5 MPa. Differences in microbial activity between the wetter and drier soil treatments following rewetting to the original water potentials are discussed in terms of the availability of energy substrate. 相似文献
Oil hydrocarbons are widespread pollutants in soil which pose serious threats to ecological environment. Thus, this study carried out the bioremediation of oil-contaminated soil by using the efficient petroleum-degrading bacteria and soil conditioner, to investigate the changes of physicochemical properties of contaminated soil during bioremediation, reveal the relationship among the exogenous degradation strains and indigenous microbe, and finally illuminate the effects of soil conditioner and microbe on the bioremediation of oil-contaminated soil.
Materials and methods
A PAH-degrading strain named Stenotrophomonas maltophilia was used in this study, which was isolated from an e-waste dismantling area. The soil conditioner in this present study was developed previously by using agricultural wastes, which was in a powdered form and rich in N, P, and K. The simulated experiments were conducted under the control environmental conditions of greenhouse, to study the effects of inoculation and soil conditioner on bioremediation of oil-contaminated soil. Then, the physicochemical properties of soil and the degradation rates of oil were measured at different set times to evaluate the bioremediation effect.
Results and discussion
Adding 1% soil conditioner could significantly improve the soil conditions and offer microorganism enough N, P, and K, which would promote microbial growth and played a key role on bioremediation of oil-contaminated soil. Although in polluted soil, the microorganism could maintain metabolic activity and use the petroleum as carbon source. The soil indigenous microbe was more easily to adapt to the contaminated surrounding. However, when both of them co-existed in soil, they would restrain each other, and go against the oil decomposition. Thus, making two types of microorganisms work to achieve synergy was the key to gain much better remediation effect. Because the indigenous microbe was good at decomposing low molecular compounds and saturated hydrocarbons, while the oil-degrading strains can effectively decompose high molecular weight aromatics.
Conclusions
The soil nutrient and microorganism, including the exogenous oil-degrading strains and the soil indigenous microbe, had an important effect on degradation of petroleum. The addition of soil conditioner, presence of indigenous microbe, and inoculation of oil-degrading strains all were conducive to bioremediation of oil-contaminated site, but the key was to control the proportion and relationship of the three.