Nitrogen Use Efficiency in an 11-Year Study of Conventional and Organic Wheat Cultivation

Abstract

Resource conservation with respect to nitrogen (N) was compared in organic and conventional cultivation of winter and spring wheat. Sustainability was measured in the nitrogen use efficiency of plant‐available N. The amounts of N entering each system and the amounts removed in the harvested crop and remaining as unused mineral nitrogen in the soil at harvest were determined. Net surpluses and losses during the growing season were also monitored, and the environmental variables influencing N harvest in the different cultivation systems were identified. The study was carried out in three different cultivation systems: conventional animal production (CONV), organic animal production (ORG1), and organic cereal production (ORG2). On average for all years and sampling occasions in winter wheat, there were approximately 60 kg more mineral nitrogen left in the soil during the growing season in CONV than in ORG1, and coefficients of variation were higher in CONV. The maximum values were considerably higher in CONV than in ORG1 (p=0.06–0.09), which increased the risk of leaching in the former, particularly in winter wheat cultivation. Nitrogen use efficiency in winter and spring wheat cultivation was 74% in whole crop conventional winter wheat and 81% in organic. Nitrogen use efficiency in harvested winter wheat grain was 44% for CONV and 49% for ORG1. ORG1 spring wheat was as efficient as ORG1 winter wheat, whereas ORG2 spring wheat used 73% of N in the whole crop and 39% in grain. Multivariate regression analysis showed that climate affected CONV and ORG1 winter wheat differently. High temperature in May increased grain yields in ORG1, but the converse was true for CONV. Large unused mineral N reserves at harvest coincided with large N harvest in CONV winter wheat. Residual fertility effects from the preceding crop produced high yields in ORG1 winter and spring wheat but had no effect in CONV. Generally, an increase in N reserves between plant development stages 13 and 31 was positive for both CONV and ORG1 winter wheat. Both winter and spring wheat require most N during this period, so the potential for improvement seems to lie in increasing mineralization (e.g., by intensified weed harrowing early in stage 13 in winter wheat and between stages 13 and 31 in spring wheat). Cultivation of winter wheat in ORG1 was a more efficient use of nitrogen resources than CONV. CONV efficiency could be improved by precision fertilization on each individual field with the help of N analysis before spring tillage and sensor‐controlled fertilization.     

Influence of industrial and alternative farming systems on contents of sugars, organic acids, total phenolic content, and the antioxidant activity of red beet (Beta vulgaris L. ssp. vulgaris Rote Kugel)

The contents of sugars, organic acids, total phenolic content, and the antioxidant activity were quantified in the flesh of red beet from conventional (CON), integrated (INT), organic (ORG), biodynamic (BD), and control farming systems using established methods. Significant differences were measured for malic acid, total phenolic content (TPC), and total antioxidant activity, where malic acid content ranged from 2.39 g kg(-1) FW (control) to 1.63 g kg(-1) FW (CON, ORG, and INT). The highest TPC was measured in BD and control samples (0.677 and 0.672 mg GAE g(-1), respectively), and the lowest in CON samples (0.511 mg GAE g(-1)). Antioxidant activity was positively correlated with TPC (r2=0.6187) and ranged from 0.823 μM TE g(-1) FW to 1.270 μM TE g(-1) FW in CON and BD samples, respectively, whereas total sugar content ranged from 21.03 g kg(-1) FW (CON) to 31.58 g kg(-1) FW (BD). The importance of sugars, organic acids, phenols, and antioxidants for human health, as well as for plant resilience and health, gained from this explorative study, is discussed and put into perspective.     

Potential impact of climate change on rainfed wheat production in Iran

Abstract

Mean monthly weather data values from 1968 – 2000 for 12 major rainfed wheat production areas in north-west and western Iran were used with a climate model, United Kingdom Meteorological Organization (UKMO), to predict the impact of climate change on rainfed wheat production for years 2025 and 2050. The crop simulation model, World Food Study (WOFOST, v 7.1), at CO₂ concentrations of 425 and 500 ppm and rising air temperature of 2.7 – 4.7°C, projected a significant rainfed wheat yield reduction in 2025 and 2050. Average yield reduction was 18 and 24% for 2025 and 2050, respectively. The yield reduction was related to a rainfall deficit (8.3 – 17.7%) and shortening of the wheat growth period (8 – 36 d). Cultivated land used for rainfed wheat production under the climate change scenarios may be reduced by 15 – 40%. Potential improvements in wheat adaptation for climate change in Iran may include breeding new cultivars and changing agronomic practices like sowing dates.     

不同蔬菜种植模式对土壤淋溶水总氮、总磷和COD的影响

以中国农业大学曲周实验站始于2002年的日光温室有机蔬菜长期定位试验为基础,采用渗漏计装置收集地下1 m深淋溶水,通过测定2014年春茬茄子与秋茬芹菜土壤淋溶水总氮、总磷和化学需氧量(COD),研究不同蔬菜种植模式(有机、综合、常规)下土壤养分淋失情况。结果表明:有机模式总氮淋失量两茬蔬菜之和为137.02 kg·hm~(-2),分别比综合和常规模式减少12.0%和25.9%;总磷淋失量两茬蔬菜之和为18.23 kg·hm~(-2),分别比综合和常规模式高51.2%和119.9%;淋溶水COD两茬蔬菜之和为856.99 kg·hm~(-2),分别比综合和常规模式高32.4%和3.1%。3种模式下不同时期淋溶水总氮、总磷与COD变化趋势差异显著。春茬茄子总氮淋失量前期维持在较高水平,追肥后出现峰值,之后迅速降低,进入6月份后一直在较低水平波动;总磷淋失量变化相对平缓,呈先增加后减小的变化趋势,6—7月间达到峰值;淋溶水COD前期稍有降低,追肥前达到最低值,进入5月份后到茄子季结束呈现逐渐上升的趋势。综上,有机种植模式在减少氮素淋失方面较综合和常规模式表现出优势,但会显著增加磷素淋失风险,并在一定程度上提高淋溶水COD。     

Influence of Microwave Heating on Biological Activities and Electrophoretic Pattern of Albumin Fraction of Wheat Grain

Microwave treatment is a sufficiently alternative technique to be applied widely in food production and cereals protection against insect pests. Water‐soluble proteins were washed out from microwave‐heated wheat grain for the purpose of assaying the influence on biological activities, reducing sugars content, and SDS‐PAGE electrophoresis proteins patterns. The differences between microwave‐heated grain samples were verified by analysis of variance at the P ≤ 0.05 level of significance. Microwave heating of wheat grain within the temperature range of 28–98°C caused a decrease in water‐extractable proteins, statistically significant when grain temperature reached 79 and 98°C. Statistically significant increase in reducing sugars content was noted in grain samples heated only to 48°C; a decrease was noted above this temperature. All biological activities studied (amylolytic and inhibition activities against α‐amylases from insects (Sithophilus granarius L., Tribolium confusum Duv., Ephestia kuehniella Zell.), human saliva, hog pancreas, antitryptic activity) were distinctly diminished in grain samples heated to 79°C. At the highest grain temperature of 98°C, the loss of all biological activities were even more pronounced due to denaturation of ≈45% of extractable proteins. Among the wheat albumins studied by SDS‐PAGE, only eight and nine protein bands were detected in the grains heated to 98 and 28°C, respectively, whereas 12 bands were present in the control. The highest number of protein bands (13) was found in the grains heated to 48 and 64°C, respectively.     

Intensive organic vegetable production increases soil organic carbon but with a lower carbon conversion efficiency than integrated management

Intensive vegetable production in greenhouses has rapidly expanded in China since the 1990s and increased to 1.3 million ha of farmland by 2016, which is the highest in the world. We conducted an 11‐year greenhouse vegetable production experiment from 2002 to 2013 to observe soil organic carbon (SOC) dynamics under three management systems, i.e., conventional (CON), integrated (ING), and intensive organic (ORG) farming. Soil samples (0–20 and 20–40 cm depth) were collected in 2002 and 2013 and separated into four particle‐size fractions, i.e., coarse sand (> 250 µm), fine sand (250–53 µm), silt (53–2 µm), and clay (< 2 µm). The SOC contents and δ¹³C values of the whole soil and the four particle‐size fractions were analyzed. After 11 years of vegetable farming, ORG and ING significantly increased SOC stocks (0–20 cm) by 4008 ± 36.6 and 2880 ± 365 kg C ha^?1 y^?1, respectively, 8.1‐ and 5.8‐times that of CON (494 ± 42.6 kg C ha^?1 y^?1). The SOC stock increase in ORG at 20–40 cm depth was 245 ± 66.4 kg C ha^?1 y^?1, significantly higher than in ING (66 ± 13.4 kg C ha^?1 y^?1) and CON (109 ± 44.8 kg C ha^?1 y^?1). Analyses of ¹³C revealed a significant increase in newly produced SOC in both soil layers in ORG. However, the carbon conversion efficiency (CE: increased organic carbon in soil divided by organic carbon input) was lower in ORG (14.4%–21.7%) than in ING (18.2%–27.4%). Among the four particle‐sizes in the 0–20 cm layer, the silt fraction exhibited the largest proportion of increase in SOC content (57.8% and 55.4% of the SOC increase in ORG and ING, respectively). A similar trend was detected in the 20–40 cm soil layer. Over all, intensive organic (ORG) vegetable production increases soil organic carbon but with a lower carbon conversion efficiency than integrated (ING) management.     

Cardinal temperatures for seed germination of wild barley,barley grass and hoary cress

It is necessary to understand the effects of environmental conditions, especially temperature, on weed seed germination patterns, in order to make comprehensive and effective weed management decisions. The effect of cardinal temperature (0°C, 5°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C and 40°C) on the germination pattern of three noxious weeds, such as wild barley (Hordeum spontaneum Koch.), barley grass (H. murinum L.) and hoary cress (Cardaria draba L.) in wheat fields of Iran, was studied in three separate experiments. The experiments were conducted based on completely randomized design (CRD) with four replications. The result showed that the germination of all three species started at low temperatures and there was no significant difference in germination percentage (GP) at lower temperatures. About 50% of the weed population germinated within a short period when using a dent-like model indicating the high ability of these weeds to compete for space and resources. Segmented and dent-like models were used to evaluate the cardinal temperatures. The results showed that dent-like model was the preferred model to evaluate germination rate in relation to variable temperatures in hoary cress, while the segmented model was better in wild barley. However, there was no significant difference between the two models for barley grass. The results of this study showed that mechanical, cultural (pre-planting irrigation) and chemical practices could be more useful and effective at 10–30°C.     

Nutrient and carbon balances in organic vegetable production on an irrigated,sandy soil in northern Oman

Our understanding of nutrient and carbon (C) fluxes in irrigated organic cropping systems of subtropical regions is limited. Therefore, leaching of mineral nitrogen (N) and phosphorus (P), gaseous emissions of NH₃, N₂O, CO₂, and CH₄, and total matter balances were measured over 24 months comprising a total cropping period of 260 d in an organic‐cropping‐systems experiment near Sohar (Oman). The experiment on an irrigated sandy soil with four replications comprised two manure types (ORG1 and ORG2) characterized by respective C : N ratios of 19 and 25 and neutral detergent fiber (NDF)‐to‐soluble carbohydrates (SC) ratios of 17 and 108. A mineral‐fertilizer (MIN) treatment with equivalent levels of mineral N, P, and potassium (K) served as a control. The three treatments were factorially combined with a cropping sequence comprising radish (Raphanus sativus L.) followed by cauliflower (Brassica oleracea L. var. botrytis) or carrot (Daucus carota subsp. sativus). Over the 24‐months experimental period gaseous N emissions averaged 45 kg ha^–1 (59% NH₃‐N, 41%N₂O‐N) for MIN, 55 kg N ha^–1 (69% NH₃‐N, 31%N₂O‐N) for ORG1, and 49 kg N ha^–1 (59% NH₃‐N, 41% N₂O‐N) for ORG2. Carbon losses were 6.2 t ha^–1 (98% CO₂‐C, 2% CH₄‐C) for MIN, 9.7 t C ha^–1 (99% CO₂‐C, 1% CH₄‐C) for ORG1, and 10.6 t ha^–1 (98% CO₂‐C, 2% CH₄‐C) for ORG2. Exchange resin–based cumulative leaching of mineral N amounted to 30 kg ha^–1 for MIN, 10 kg ha^–1 for ORG1, and 56 kg ha^–1 for ORG2. Apparent surpluses of 361 kg N ha^–1 and 196 kg P ha^–1 for radish‐carrot and 299 kg N ha^–1 and 184 kg P ha^–1 for radish‐cauliflower were accompanied by K deficits of –59 kg ha^–1 and –73 kg ha^–1, respectively, for both cropping systems. Net C balances for MIN, ORG1, and ORG2 plots were –7.3, –3.1, and 1.5 t C ha^–1 for radish‐carrot and –5.0, 1.3, and 4.6 t C ha^–1 for radish‐cauliflower. The results underline the difficulty to maintain soil C levels in intensively cultivated, irrigated subtropical soils.     

Some factors affecting survival of sclerotia of Macrophomina phaseolina in soil

At least 75% of the sclerotia of Macrophomina phaseolina survived for 1 yr in most natural soils kept at 26°C and at 50–55% of the soil moisture holding capacity (m.h.c.). Although survivability was reduced in a very acid soil (pH 4.5) collected under a pine stand, 33% of the sclerotia survived for 1 yr. Soil pH had very little or no effect on sclerotial survivability. Of three organic amendments tested (alfalfa hay, chitin, pine needles) only ground alfalfa hay at 0.8% (w/w) reduced survivability of sclerotia in soil by about 75% in a year. Alfalfa hay at 0.4% reduced survivability by 36%. Various N sources added at 200 μg Ng^?1 soil had no effect on survival. Of 13 fungicides tested, only benomyl and captan at 20 μg a.i. g^?1 soil appreciably reduced populations of sclerotia in soil.Soil temperature and moisture content were the two most important factors affecting survivability of sclerotia. At ?5 or 5°C the biggest drop in sclerotial survivability occurred when the soil was incubated moist (at 50% m.h.c. or more). At 26°C the biggest drop occurred in air-dried soil (2–3% m.h.c.) and survivability was decreased to some extent at 15 and 30% m.h.c. Survivability also dropped rapidly in moist soil (50–55% m.h.c.) exposed to four cycles each having 3-week freezing (?5°C) and 1 week thawing (26°C). Sclerotia in air-dried soil (2–3% m.h.c.) continuously kept at ?5°C maintained nearly complete survivability after 16 weeks. Sclerotia survived almost 80–90% in moist soil (50–55% m.h.c.) kept for 16 weeks at 26°C or in moist soil exposed to four cycles each having 3-week thawing (26°C) and 1-week freezing (?5°C).     

Fate of Fusarium graminearum and Other Fusarium Species During Composting Of Beef Cattle Feedlot Manure

Fusarium head blight, caused by Fusarium graminearum, could potentially become a major concern for the cereal industry in Alberta, Canada. Infested feed grain in feedlot manure may act as a means of spreading the disease when manure is land applied. The ability of manure composting to eradicate the pathogen on infested grain (wheat, barley, corn) was evaluated. F. graminearum and other Fusarium spp. were rapidly eradicated from infested grains buried in compost windrows with no recovery after 2 d where windrow temperature attained 51°C. Under cooler windrow conditions (11.9 to 17.5°C), recovery of F. graminearum reached zero on Day 9 for corn (Zea mays L.), Day 14 for wheat (Triticum aestivum L.) and Day 22 for barley (Hordeum vulgare L.), showing that factors in addition to compost temperature may play a role in pathogen elimination. Composting represents an effective strategy in mitigating the dissemination of F. graminearum via manure should land application occur on fields that are subsequently used for grain production.     

Supra‐optimal growth temperature exacerbates adverse effects of low Zn supply in wheat

Rising temperatures are a major threat to global wheat production, particularly when accompanied by other abiotic stressors such as mineral nutrient deficiencies. This study aimed to quantify the effects of supra‐optimal temperature on growth, photosynthetic performance, and antioxidative responses in bread wheat cultivars grown under varied zinc (Zn) supply. Two bread wheat cultivars (Triticum aestivum L., cvs. Lasani‐2008 and Faisalabad‐2008) with varied responsiveness to Zn supply and drought tolerance were cultured in nutrient solution with low (0.1 µM) or adequate (1.0 µM) Zn under optimal (25/20°C day/night) or supra‐optimal (36/28°C day/night) temperature regimes. Supra‐optimal temperature severely reduced root but not shoot biomass, whereas low Zn reduced shoot as well as root biomass. Shoot‐to‐root biomass ratio was reduced under low Zn but increased under supra‐optimal temperature. Supra‐optimal temperature inhibited root elongation and volume particularly in plants supplied with low Zn. In both cultivars, Zn efficiency index was reduced by supra‐optimal temperature, whereas heat tolerance index was reduced by low Zn supply. Supra‐optimal temperature decreased photosynthesis, quantum yield, and chlorophyll density in low‐Zn but not in adequate‐Zn plants. In comparison, low Zn decreased specific activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) and increased glutathione reductase (GR), where supra‐optimal temperature increased SOD, decreased GR and did not change APX activity in leaves and roots. Moreover, supra‐optimal temperature severely reduced shoot Zn concentration and Zn uptake per plant specifically under adequate Zn supply. Overall, supra‐optimal temperature exacerbated adverse effects of low Zn supply, resulting in severe reductions in growth traits viz. shoot and root biomass, root length and volume, and consequently impeded Zn uptake, enhanced oxidative stress and impaired photosynthetic performance. Adequate Zn nutrition is crucial to prevent yield loss in wheat cultivated under supra‐optimal temperatures.     

Influence of Loss‐on‐Ignition Temperature and Heating Time on Ash Content of Compost and Manure

Abstract

Loss‐on‐ignition (LOI) is a simple method for determining ash content, and by reciprocation, organic matter content of compost and manure. However, reported ignition temperatures and heating times for LOI measurements vary widely, and this brings into question the accuracy of one specific combination of ignition temperature and heating time over another. This study examined the effect of 42 temperature‐heating time combinations (six ignition temperatures from 400 to 650°C in 50°C increments by seven heating times of 1, 2, 8, 12, 16, 20, and 24‐h) on the ash content of a finished compost and a fresh manure. The experiment included the 550°C for 2‐h method recommended in Test Methods for Evaluation of Compost and Composting. The magnitude of the decrease in ash content due to increase in temperature was not consistent across all heating times. For example, after a 1‐h heating time for compost, ash content was 75.7% at 400°C and 67.5% at 650°C, compared to 69.6% at 400°C and 66.8% at 650°C after 24‐h. Irrespective of heating time, an ignition temperature of 400°C overestimated ash content for both compost and manure compared to the TMECC method. The TMECC method with its moderate temperature and short heating time requirement could reduce energy costs without affecting ash content results.     

Soil organic matter dynamics under grain farming in Northern Kazakhstan

Because of their ability to store a high amount of soil organic matter (SOM), Chernozem soils are one of the most important resources from both agricultural and environmental viewpoints. This study was carried out to determine the SOM budget under grain farming in the Chernozem soil of northern Kazakhstan through the analysis of in situ soil respiration and soil environmental factors such as soil temperature as well as moisture content. Five experimental plots including one fallow field were established at the experimental farm of Barayev Kazakh Research and Production Center of Grain Farming, Shortandy, northern Kazakhstan (mean annual precipitation and average year temperature are 323 mm and 1.6°C, respectively). Mean daily soil temperature increased to above O°C in early April, remaining at above 20°C from mid-June to mid-August, and then sharply decreased to below 5°C at the end of September. Most of the biological activities were considered to be limited from April to September. On the other hand, the soil moisture content remained high after thawing until mid-June and then continuously decreased in the cropped plots except during the rainfall events. The soil respiration rate recorded the highest values from late June to early July and overall fluctuations were similar to those of the soil temperature, unlike the fluctuations of soil microbial C and N contents, which exhibited similar patterns to those of the soil moisture content. In order to represent the daily soil respiration rates using the soil environmental factors, the following relationship was introduced as a model function: Cem = aM ^pbexp(-E/RT). The coefficients, a, b, and E (activation energy in Arrhenius equation), were determined by stepwise multiple regression after logarithm transformation using the measured data, Cem (daily soil respiration rate), M (volumetric soil moisture content), and T (absolute soil temperature). As a result, a significant relationship was always obtained between the soil respiration rate and the activation energy, E, while the contribution of the soil moisture content to the soil respiration rate was uncertain. Using the regression equations and monitored data of soil temperature and moisture content, cumulative soil respiration throughout the cropping period was calculated to be in the range of 2.5 to 3.2 Mg C ha^p-1 On the other hand, the amounts of crop residues in the cropped plots that were expected to be incorporated into the soils ranged from 1.6 to 4.4 Mg C ha^p-1 Except for the plot planted with oats (higher amounts of residues than for wheat), the SOIL budget was slightly negative in this year, that is, the soils lost their organic matter stock. Although it is difficult to generalize the C budget in different years because of the large variations in crop growth due to fluctuating water resources, the disadvantage of summer fallow (no residues) was obvious in terms of SOM budget. The net soil respiration rate in the fallow plot, 2.9 Mg C ha^p-1 was approximately equivalent to 4% of the total SOM stock in the plow layer (30 cm) (70 to 80 Mg C ha^p-1 To reduce further loss of SOM, at least evenly extensive use of summer fallow should be reconsidered.     

Effect of Ultrasonic Treatment of Brown Rice at Different Temperatures on Cooking Properties and Quality

This research studied developing quick cooking brown rice by investigating the effect of ultrasonic treatment at different temperatures on cooking time and quality. The medium grain brown rice was ultrasonically treated in water at temperatures of 25, 40, and 55°C for 30 min and then dried by air at 25°C to its initial moisture content (11.0 ± 0.6%, wb) before cooking. The microstructure of rice kernel surface, chemical composition, and optimal cooking time of treated brown rice were determined. The pasting and thermal properties and chemical structure of flour and starch from treated brown rice were also examined. The results showed that the optimal cooking times were 37, 35, and 33 min after treatment at 25, 40, and 55°C, respectively, compared to the control of 39.6 min. The ultrasonic treatment resulted in a loss in natural morphology of rice bran, allowing water to be absorbed by a rice kernel easily, particularly at high‐temperature treatment. Even through rice flour still maintained an A‐pattern in the pasting properties, the crystallinity significantly increased after treatment at 55°C. Ultrasonic treatment increased the peak, hold, and final viscosities and decreased the onset temperature (T_o) and peak temperature (T_p), significantly. Thus, ultrasonic treatment could be used for reducing cooking time of brown rice.     

Use of IR thermography in screening wheat (Triticum aestivum L.) cultivars for salt tolerance

Thermography is proposed to be an alternative non-destructive and rapid technique for the study and diagnosing of salt tolerance in plants. In a pot experiment, 30 cultivars of wheat (Triticum aestivum L.) were evaluated in terms of their leaf temperature and shoot growth and their ion distribution responses to NaCl salinity at two concentration levels: the control with electrical conductivity (EC) of 1 dS m^?1 and salinity treatment with EC of 16 dS m^?1 (150 mM). A completely randomized block design with factorial treatments was employed with three replications. The results indicated that thermography may accurately reflect the physiological status of salt-stressed wheat plants. The salt stress-based increase in leaf temperature of wheat cultivars grown at 150 mM NaCl reached 1.34°C compared to the control. According to the results obtained, it appears that thermography has the capability of discerning differences of salinity tolerance between the cultivars. Three salt-tolerant wheat cultivars, namely Roshan, Kharchia and Sholeh, had higher mean shoot dry matter (0.039 g plant^?1) and higher mean ratio of leaf K⁺/Na⁺ (14.06) and showed lower increase in the mean leaf temperature (0.37°C) by thermography compared to the control. This was while nine salt-sensitive cultivars, namely Kavir, Ghods, Atrak, Parsi, Bahar, Pishtaz, Falat, Gaspard and Tajan, had lower mean plant dry matter production (0.027 g plant^?1), lower mean ratio of K⁺/Na⁺ (9.49) and higher mean increases in leaf temperature (1.24°C).     

Quality studies on wheat grown in different cropping systems: a holistic perspective

Spring wheat from a conventional (CONV) and an organic (ORG1) cropping system, both with animals, and from an organic system without animals (ORG2) was evaluated with respect to baking quality for the years 1995–2002. Amino acid (AA) composition was studied in both spring and winter wheat in 1993 and 2000–2002. The data were combined in multivariate analysis for exploration of the main factors responsible for the variation in quality. The most important factor for baking quality was weather conditions. High rainfall in May favoured baking quality in both cropping systems with animals, as did high temperature in May and high rainfall in July in the ORG1 system, and low rainfall in August in the CONV system. The only significant difference between the cropping systems was falling number, which was higher in ORG1 (252 s) than in CONV (205 s), probably due to a heavier CONV crop stand causing more difficult drying conditions. AA composition differed more between years than between cropping systems for both winter and spring wheat. The content of essential amino acids was high under the weather conditions associated with poor baking quality. The contents of threonine and leucine in spring wheat were significantly higher in ORG1, 1.76 and 8.11 g/100 g crude protein than in CONV, 1.63 and 7.72, respectively. In the interaction between AA and baking quality in spring wheat, it was possible to determine a correlation between phenylalanine, histidine, lysine and good baking properties. The primary effect was associated with weather conditions, but there was also an effect of differences between the cropping systems.     

Nitrous oxide production in aerobic soils under varying pH,temperature and water content

Laboratory studies were conducted to evaluate the effect of soil pH, temperature and water content on the rate of nitrification and on the amount of N₂O evolved from samples of Plano silt loam soil. The rate of nitrification of added NH₄⁺-N increased with increasing soil pH (4.7, 5.1 and 6.7), temperature (10, 20 and 30°C) and water content (0.1, 0.2 and 0.3 m³ m^?3). At soil water contents of 0.1 and 0.2 m³ m^?3, corresponding to 18 and 36% water-filled pore space, respectively, N₂O evolution was proportional to NO₃^? production. Approximately 0.1–0.2% of the nitrified N was evolved as N₂O-N. At 0.3 m³ m^?3 water content (54% water-filled pore space) and 20 and 30°C, the ratio of N₂O-N evolved to N nitrified was significantly higher (range of 0.3–1.1%).An additional experiment was conducted using diurnally fluctuating temperatures (10–30°C). The pattern of N₂O evolution was markedly different when the system was sampled at 10 and 30°C than at 20°C. The apparent N₂O emission rates were approximately equal for 12-h periods during which the temperature increased from 10 to 30°C or decreased from 30 to 10°C. In contrast, the apparent N₂O emission rates were significantly lower for the 12-h period when the incubation flasks were sampled at 20°C following the daily minimum temperature compared to the 12-h period when the samplings were at 20°C following the daily maximum temperature. This provides additional evidence that temperature fluctuation in the surface soil is a factor in-observed diurnal variations in N₂O emissions under field conditions.Our findings indicate that an interaction of three factors (soil pH, temperature and water content) affects the amount of N₂O evolved during nitrification in soils. In relatively dry soils, estimated N₂O production of ca. 0.1–0.3% of the N nitrified may be sufficiently accurate. Much higher N₂O output can be expected following rainfall or irrigation. Diurnal variability in N₂O fluxes from soils due to fluctuating temperature is an additional uncertainty in quantifying N₂O production in field soils.     

Glomalin in aggregate size classes from three different farming systems

Glomalin was measured in soil from farming systems managed for 8 years by chisel tillage (CT), more intensive tillage for organic (ORG) production, and no tillage (NT) on Acrisols (FAO Soil Units) in the Mid-Atlantic region of the U.S. Whole soil and aggregate size classes of >2.00, 0.50–2.00 and 0.21–0.50 mm (macroaggregates), 0.05–0.21 mm (microaggregates), and <0.05 mm (fine material) were examined. Glomalin-related soil protein (GRSP) was extracted from 1-g samples (four plots per treatment) with 100 mM sodium pyrophosphate, pH 9.0, at 121 °C in three extraction cycles. Extracts were pooled and quantified by using the Bradford protein assay. Concentrations of GRSP and total carbon (C) in aggregates were linearly related across aggregate size classes for all treatments (GRSP = 0.101C + 0.56, r² = 0.95). No tillage had significantly greater whole soil GRSP than did CT or ORG (P = 0.01). Mean values for GRSP in aggregates of NT were higher than for CT or ORG aggregates by 0.53 and 0.66 mg g⁻¹ aggregates, respectively. There were no differences among treatments in GRSP concentrations in fine material. In NT the concentration of GRSP increased as aggregate size increased in contrast to the disturbed treatments, CT or ORG, where there were no differences in GRSP concentration across aggregate size fractions. Larger proportions of GRSP were distributed in macroaggregates of NT compared to CT and ORG in contrast to larger proportions in microaggregates of CT and ORG than in NT. Although soil disturbance in ORG farming is greater than for CT farming, both treatments had similar GRSP concentrations and distributions.     

Thermal stabilities of urease enzymes in some irish soils

The thermal stabilities of urease enzymes in six re-wetted, air-dry soils were characterized and compared by incubation at 4, 37 and 70°C. Inactivation rates were dependent on temperature and soil type. Calculated half-lives (t_1/2 values) of soil urease were 384–768 h at 4°C, 79–229 h at 37°C and <2–6 h at 70°C. At 37°C, soil urease decay patterns were best described by first-order kinetics.Half-life values (t_1/2) at 37°C were significantly correlated with soil pH values (r = 0.944; P < 0.05) but less strongly with organic-C (r = 0.699; NS) and CEC (r = 0.671; NS). Almost all (98.9%; P < 0.05) of the variation in t_1/2 values was accounted for by a multiple regression equation incorporating the foregoing variables.     

Increase in Apparent Amylose Content and Change in Starch Pasting Properties at Cool Growth Temperatures in Mutant Wheat

Some mutant wheat lines with low‐amylose content were grown in a field and greenhouse (15 or 20°C) to compare apparent amylose content and starch pasting properties. The apparent amylose content of flour and starch increased and starch pasting parameters as measured by a Rapid Visco Analyser (RVA) changed in the greenhouse (at cool temperatures) during seed maturation. Densitometric analysis of the protein band separated by electrophoresis suggested that the increase in amylose content by cool temperature was related to the amount of Wx‐D1 protein. This data suggests that the Wx‐D1 gene was responsible for these changes. In wheat starch from Tanikei A6099 and Tanikei A6598 at 15°C, the value of final viscosity and total setback was higher than that from the field. In wheat starch from Tanikei A6599‐4 (waxy mutant with stable hot paste viscosity), the peak viscosity temperature was higher and time maintained >80% of the peak was shorter at 15°C than that from the field. Genetic analysis using doubled‐haploid (DH) lines from a combination of Tanikei A6599‐4 and Kanto 118 (low‐amylose line) showed that apparent amylose content increased and the starch pasting curve and properties changed in waxy progenies similar to Tanikei A6599‐4.