半干旱地区土壤水分和养分对玉米生长和产量的耦合影响

Interaction between soil water and nutrients plays an important role in sustainable crop management in semi-arid environments.On the basis of a field experiment conducted from 2000 to 2003,this study examined the coupled effects of irrigation and fertilizers on maize growth and yield in a semi-arid region of northeastern China.In terms of plant productivity,nitrogen fertilizer had the most significant effect followed by irrigation and phosphate levels.The combined application of nutrients and irrigation exerted a synergistic effect on the grain yield of maize plants.Regression analysis indicated that optimal levels of nitrogen and phosphate,in addition to adequate irrigation,could greatly improve the efficiency of grain production.Similarly,optimization of soil nutrient availability substantially increased water use efficiency.These suggested that for the most efficient and sustainable crop production,irrigation and nutrient management should be based on a quantitative understanding of water/nutrients interaction,particularly in semi-arid and arid regions.     

长期施肥对华北平原土壤生产力的影响

Soil productivity is the ability of a soil, in its normal environment, to support plant growth and can be evaluated with respect to crop production in unfertilized soil within the agricultural ecosystem. Both soil productivity and fertilizer applications affect crop yields. A long-term experiment with a winter wheat-summer maize rotation was established in 1989 in a field of the Fengqiu State Key Agro-Ecological Experimental Station, a region typical of the North China Plain, including seven treatments: 1) a balanced application of NPK chemical fertilizers(NPK); 2) application of organic fertilizer(OM); 3) application of 50% organic fertilizer and50% NPK chemical fertilizers(1/2OMN); 4) application of NP chemical fertilizers(NP); 5) application of PK chemical fertilizer(PK);6) application of NK chemical fertilizers(NK); and 7) unfertilized control(CK). To investigate the effects of fertilization practices on soil productivity, further pot tests were conducted in 2007–2008 using soil samples from the different fertilization treatments of the long-term field experiment. The soil sample of each treatment of the long-term experiment was divided into three pots to grow wheat: with no fertilization(Potunf), with balanced NPK fertilization(PotNPK), and with the same fertilizer(s) of the long-term field experiment(Potori). The fertilized soils of the field experiment used in all the pot tests showed a higher wheat grain yield and higher nutrient uptake levels than the unfertilized soil. Soil productivity of the treatments of the field experiment after 18 years of continuous fertilizer applications were ranked in the order of OM 1/2OMN NPK NP PK NK CK. The contribution of soil productivity of the different treatments of the field experiment to the wheat grain yield of Potoriwas 36.0%–76.7%, with the PK and NK treatments being higher than the OM, 1/2OMN, NPK, and NP treatments since the soil in this area was deficient in N and P and rich in K. Wheat grain yields of PotNPKwere higher than those of Potoriand Potunf. The N, P, and K use efficiencies were higher in PotNPKthan Potoriand significantly positively correlated with wheat grain yield. Soil organic matter could be a better predictor of soil productivity because it correlated more strongly than other nutrients with the wheat grain yield of Potunf. Wheat yields of PotNPKshowed a similar trend to those of Potunf, indicating that soil productivity improvement was essential for a further increase in crop yield. The long-term applications of both organic and chemical fertilizers were capable of increasing soil productivity on the North China Plain, but the former was more effective than the latter. The balanced application of NPK chemical fertilizers not only increased soil productivity, but also largely increased crop yields, especially in soils with lower productivity. Thus, such an approach should be a feasible practice for the sustainable use of agricultural soils on the North China Plain, particularly when taking into account crop yields, labor costs, and the limited availability of organic fertilizers.     

营养和病虫害管理对双季杂交水稻种植系统的土壤生态质量的共同影响

The mineral fertilizers(NPK) and pesticide,including herbicides,insecticides and fungicides,were applied alone or in combination and the soil sampling was done at different growth stages during the crop cycle to study the changes in soil organic matter,microbial biomass and their activity parameters in a paddy soil with different nutrient and pest management practices in a hybrid rice double-cropping system.A consistent increase in the electron transport system(ETS) activity was measured during the different crop growth stages of rice.The use of fertilizers(NPK) alone or with pesticides increased ETS activity,while a decline of ETS activity was noticed with pesticides alone as compared with the control.Nearly an increasing trend in soil phenol content was observed with the progression of crop growthstages,while the usage of pesticides alone caused maximum increments in the soil phenol content.The soil protein content was found nearly stable with fertilizers and/or pesticides application at various growth stages in both crops taken.But notable changes were noticed at different growth stages probable because of fluctuations in moisture and temperature at particular stages,which might have their effects on N mineralization.Marked depletions in the phospholipid content were found with the advancement of crop growth stages,while the incorporation of fertilizers and/or pesticides also produced slight changes,in which a higher decline was noticed with pesticide application alone compared with the control.     

中国小麦田土壤线虫对生物炭添加的响应

While studies have focused on the use of biochar as soil amendment, little attention has been paid to its effect on soil fauna. The biochar was produced from slow pyrolysis of wheat straw in the present study. Four treatments, no addition (CK) and three rates of biochar addition at 2 400 (B1), 12 000 (B5) and 48 000 kg ha-1 (B20), were investigated to assess the effect of biochar addition to soil on nematode abundance and diversity in a microcosm trial in China. The B5 and B20 application significantly increased the total organic carbon and the C/N ratio. No significant difference in total nematode abundance was found among the treatments. The biochar addition to the soil significantly increased the abundance of fungivores, and decreased that of plant parasites. The diversity of soil nematodes was significantly increased by B1 compared to CK. Nematode trophic groups were more effectively indicative to biochar addition than total abundance.     

Leaching and Transformation of Nitrogen Fertilizers in Soil After Application of N with Irrigation： A Soil Column Method

A soil column method was used to compare the effect of drip fertigation （the application of fertilizer through drip irrigation systems, DFI） on the leaching loss and transformation of urea-N in soil with that of surface fertilization combined with flood irrigation （SFI）, and to study the leaching loss and transformation of three kinds of nitrogen fertilizers （nitrate fertilizer, ammonium fertilizer, and urea fertilizer） in two contrasting soils after the fertigation. In comparison to SFI, DFI decreased leaching loss of urea-N from the soil and increased the mineral N （NH4^＋-N ＋ NO3^--N） in the soil. The N leached from a clay loam soil ranged from 5.7% to 9.6% of the total N added as fertilizer, whereas for a sandy loam soil they ranged between 16.2% and 30.4%. Leaching losses of mineral N were higher when nitrate fertilizer was used compared to urea or ammonium fertilizer. Compared to the control （without urea addition）, on the first day when soils were fertigated with urea, there were increases in NH4^＋-N in the soils. This confirmed the rapid hydrolysis of urea in soil during fertigation. NH4^＋-N in soils reached a peak about 5 days after fertigation, and due to nitrification it began to decrease at day 10. After applying NH4^＋-N fertilizer and urea and during the incubation period, the mineral nitrogen in the soil decreased. This may be related to the occurrence of NH4^＋-N fixation or volatilization in the soil during the fertigation process.     

聚合果属生物炭引起的咸水灌溉下土壤养分有效性和番茄生长变化

Thermally modified organic materials commonly known as biochar have gained popularity of being used as a soil amendment.Little information, however, is available on the role of biochar in alleviating the negative impacts of saline water on soil productivity and plant growth. This study, therefore, was conducted to investigate the effects of Conocarpus biochar(BC) and organic farm residues(FR) at different application rates of 0.0%(control), 4.0% and 8.0%(weight/weight) on yield and quality of tomatoes grown on a sandy soil under drip irrigation with saline or non-saline water. The availability of P, K, Fe, Mn, Zn and Cu to plants was also investigated. The results demonstrated clearly that addition of BC or FR increased the vegetative growth, yield and quality parameters in all irrigation treatments. It was found that salt stress adversely affected soil productivity, as indicated by the lower vegetative growth and yield components of tomato plants. However, this suppressing effect on the vegetative growth and yield tended to decline with application of FR or BC, especially at the high application rate and in the presence of biochar. Under saline irrigation system, for instance, the total tomato yield increased over the control by 14.0%–43.3% with BC and by 3.9%–35.6% with FR. These could be attributed to enhancement effects of FR or BC on soil properties, as indicated by increases in soil organic matter content and nutrient availability. Therefore, biochar may be effectively used as a soil amendment for enhancing the productivity of salt-affected sandy soils under arid conditions.     

海河流域合理生态用水比例的确定

Winter wheat-maize rotations are dominant cropping systems on the North China Plain, where recently the use of organic manure with grain crops has almost disappeared. This could reduce soil fertility and crop productivity in the long run. A 20-year field experiment was conducted to 1） assess the effect of inorganic and organic nutrient sources on yield and yield trends of both winter wheat and maize, 2） monitor the changes in soil organic matter content under continuous wheat-maize cropping with different soil fertility management schemes, and 3） identify reasons for yield trends observed in Xuzhou City, Jiangsu Province, over a 20-year period. There were eight treatments applied to both wheat and maize seasons： a control treatment （C）; three inorganic fertilizers, that is, nitrogen （N）, nitrogen and phosphorus （NP）, and nitrogen, phosphorus and potassium （NPK）; and addition of farmyard manure （FYM） to these four treatments, that is, M, MN, MNP, and MNPK. At the end of the experiment the MN, MNP, and MNPK treatments had the highest yields, about 7 t wheat ha^-1 and 7.5 t maize ha^-1, with each about 1 t ha^-1 more than the NPK treatments. Over 20 years with FYM soil organic matter increased by 80% compared to only 10% with NPK, which explained yield increases. However, from an environmental and agronomic perspective, manure application was not a superior strategy to NPK fertilizers. If manure was to be applied, though, it would be best applied to the wheat crop, which showed a better response than maize.     

铅对两个红壤中酶活性的影响

Enzyme activities have the potential to indicate biological functioning of soils. In this study, soil urease, dehydrogenase, acid phosphatase and invertase activities and fluorescein diacetate（FDA） hydrolysis were measured in two red soils spiked with Pb^2＋ ranging from 0 to 2 400 mg kg^-1 to relate the enzyme activity values to both plant growth and the levels of available and total Pb^2＋ concentrations in soils, and to examine the potential use of soil enzymes to assess the degrees of Pb contamination. Soil samples were taken for enzyme activities assaying during 3 month’s incubation and then after planting of celery（Apium graveolens L.） and Chinese cabbage（Brassica chinensis L.）. Enzyme activities in the red soil derived from arenaceous rock（RAR） were generally lower than those in the red soil developed on Quaternary red earths（REQ）. At high Pb^2＋ loadings, in both incubation and greenhouse studies, urease activity and FDA hydrolysis were significantly inhibited. But there were no significant relationships between soil dehydrogenase, acid phosphatase or invertase activity and soil Pb^2＋ loadings in both RAR and REQ soils. The growth of celery and Chinese cabbage increased soil urease activity and FDA hydrolysis, but had minimal effect on dehydrogenase and invertase activities. There were positive correlations between celery biomass and soil urease activity and FDA hydrolysis. These results demonstrate that urease activity and FDA hydrolysis are more sensitive to Pb^2＋ than acid phosphatase, dehydrogenase and invertase activities in the RAR and REQ soils.     

不同土地利用方式下上壤微生物群落水平生理图谱(CLP)与土壤碳、氮有效性之间的相互关系（英文）

The goal of this work was to assess soil microbial respiration,determined by the assay of community-level physiological profiling in an oxygen-sensitive microplate(O2-CLPP),in response to endogenous C and several individual C substrates in the soils with different organic C contents(as a function of soil type and management practice).We also used the O2-CLPP to determine the respiratory response of these soils to endogenous C and amended C substrates with N addition.A respiratory quotient(RQ) was calculated based on the ratio of the response to endogenous soil C vs.each C-only substrate,and was related to total organic carbon(TOC).For assessing N availability for microbial activity,the effect of N supplementation on soil respiration,expressed as N_(ratio),was calculated based on the response of several substrates to N addition relative to the response without N.Soils clustered in 4 groups after a principal component analysis(PCA),based on TOC and their respiratory responses to substrates and endogenous C.These groups reflected differences among soils in their geographic origin,land use and C content.Calculated RQ values were significantly lower in natural forest soils than in managed soils for most C-only substrates.TOC was negatively correlated with RQ(r = —0.65),indicating that the soils with higher organic matter content increased respiratory efficiency.The N addition in the assay in the absence of C amendment(i.e.,only endogenous soil C present) had no effect on microbial respiration in any soil,indicating that these soils were not intrinsically N-limited,but substrate-dependent variation in N_(ratio) within soil groups was observed.     

西藏高原自然生态系统转化为农田后提高了土壤的净矿化和净硝化

A comparative study was conducted to determine the NH4^＋ and NO3^- concentrations in soil profiles and to examine the net nitrogen （N） mineralization and nitrification in adjacent forest, grassland, and cropland soils on the Tibetan Plateau. Cropland soil showed significantly higher inorganic N concentrations in soil profiles compared with forest and grassland soils. NO3^- -N accounted for 70%-90^ of inorganic N in cropland soil, while NH4^＋ -N was the main form of inorganic N in forest and grassland soils. The average net N mineralization rate at 0 20 cm depth was approximately twice in cropland soil （1.48 mg kg^-1 d^-1） as high as in forest （0.83 mg kg^-1 d^-1） or grassland soil （0.72 mg kg^-1 d^-1）. Cropland showed strong net nitrification, with the net rate almost equal to the total net N mineralization. Urea addition stimulated soil respiration, particularly in forest soil. Most urea-N, however, remained as NH4^＋ in forest and grassland soils, while NO3^- was the main form of inorganic N to increase in cropland soil. Higher rates of net nitrification in cropland soils suggest that land use change on the Tibetan Plateau may lead to high N losses through nitrate leaching.     

施入~(15)N标记氮肥在长期不同培肥土壤的残留及其利用

采用盆栽试验和短期矿化培养相结合的方法,研究了施入15N标记氮肥(+N)及其与秸秆配施(+1/2N+1/2S)在3种长期(19年)不同培肥土壤(即:No-F,长期不施肥土壤;NPK,长期施用NPK化肥土壤;MNPK,长期有机无机肥配施土壤)中的残留及其矿化和作物吸收特性。结果表明,第一季小麦收获后,+1/2N+1/2S处理下三供试土壤和+N处理下的NPK和MNPK土壤残留肥料氮(残留15N)中有82.6%~95.1%以有机态存,而+N处理下No-F土壤残留15N有47.7%以矿质态存在。经过28 d矿化培养后,与NPK土壤相比,MNPK土壤氮素净矿化量显著增加,增幅为39%~49%;NPK和MNPK土壤残留肥料氮(残留15N)矿化量为1.23~1.90 mg kg-1,占总残留15N的2.78%~5.53%,均显著高于No-F土壤。与+N处理相比,+1/2N+1/2S处理显著提高了3供试土壤氮素净矿化量,但两施肥处理对NPK和MNPK土壤残留15N矿化量无显著影响。+N处理下No-F土壤残留15N的利用率为20%,显著高于NPK(9%)和MNPK(12%)土壤。两种施肥处理下,MNPK土壤残留15N的利用率均显著高于NPK土壤。短期培养期间土壤氮素矿化量和第二季小麦生育期作物吸氮量呈显著性正相关,而残留15N矿化量和第二季小麦吸收残留15N量间无显著性相关关系。长期有机无机配施可以提高土壤残留肥料氮的矿化量及有效性。     

Benefits of Biochars and NPK Fertilizers for Soil Quality and Growth of Cowpea (Vigna unguiculata L. Walp.) in an Acid Arenosol

Fertilization is required for optimum plant growth, particularly in unfertile soils, while optimizing nutrient use efficiency is an alternative to reduce inorganic fertilizer needs and reduce environmental problems caused by nutrient leaching. This study investigated soil properties and cowpea yield responses to biochars (BCs) made from different feedstocks, baby corn peel biochar (BC1), branches of mango tree biochar (BC2), and rice husk biochar (BC3), applied in combination with nitrogen-phosphorus-potassium (NPK) fertilizers. The experiment was conducted in a greenhouse, using an acid sandy soil (Arenosol) that was submitted for 70 d to the following eight treatments:i) control; ii) full dose of NPK (a commercial compound fertilizer (12-24-12 of N-P₂O₅-K₂O) + urea (46% N)); iii) BC1 + half dose of NPK; iv) BC1 + full dose of NPK; v) BC2 + half dose of NPK; vi) BC2 + full dose of NPK; vii) BC3 + half dose of NPK; and viii) BC3 + full dose of NPK. All biochars were applied at a rate of 0.9% (weight/weight), and each type of biochar was combined with half and full doses of NPK fertilizers. Soil pH increased significantly (P < 0.05) in treatments with BC1 and BC2, while cation exchange capacity (CEC) and available P were higher in the treatments with BC1; BC1 and BC2 also induced higher activity of enzymes related to the P cycle and higher cowpea yield. Similar soil properties and cowpea yield parameters were obtained with the full and half doses of NPK fertilizers for each type of biochar used. In conclusion, biochars in the combination with NPK fertilizers improved soil chemistry and enzymatic activities, allowing reduced fertilizer application and food production costs in the acid soil studied.     

Long‐term field fertilization affects soil nitrogen transformations in a rice‐wheat‐rotation cropping system

Mineralization and nitrification are the key processes of the global N cycle and are primarily driven by microorganisms. However, it remains largely unknown about the consequence of intensified agricultural activity on microbial N transformation in agricultural soils. In this study, the ¹⁵N‐dilution technique was carried out to investigate the gross mineralization and nitrification in soils from a long‐term field fertilization experiment starting from 1988. Phospholipid fatty acids (PLFA) analysis was used to determine soil microbial communities, e.g., biomasses of anaerobic bacterial, bacterial, fungi, and actinobacteria. The abundance of ammonia‐oxidizing bacteria (AOB) and archaea (AOA) were measured using real‐time quantitative polymerase chain reaction. The results have demonstrated significant stimulation of gross mineralization in the chemical‐fertilizers treatment (NPK) ([6.53 ± 1.29] mg N kg^–1 d^–1) and chemical fertilizers–plus–straw treatment (NPK+S1) soils ([8.13 ± 1.68] mg N kg^–1 d^–1) but not in chemical fertilizers–plus–two times straw treatment (NPK+S2) soil when compared to the control‐treatment (CK) soil ([3.62 ± 0.86] mg N kg^–1 d^–1). The increase of anaerobic bacterial biomass is up to 6‐fold in the NPK+S2 compared to that in the CK soil ([0.7 ± 0.5] nmol g^–1), implying that exceptionally high abundance of anaerobic bacteria may inhibit gross mineralization to some extent. The gross nitrification shows upward trends in the NPK+S1 and NPK+S2 soils. However, it is only significantly higher in the NPK soil ([5.56 ± 0.51] mg N kg^–1 d^–1) compared to that in the CK soil ([3.70 ± 0.47] mg N kg^–1 d^–1) (p < 0.05). The AOB abundance increased from (0.28 ± 0.07) × 10⁶ copies (g soil)^–1 for the CK treatment to (4.79 ± 1.23) × 10⁶ copies (g soil)^–1 for the NPK treatment after the 22‐year fertilization. In contrast, the AOA abundance was not significantly different among all treatment soils. The changes of AOB were well paralleled by gross nitrification activity (gross nitrification rate = 0.263 AOB + 0.047 NH ‐N + 2.434, R² = 0.73, p < 0.05), suggesting the predominance of bacterial ammonia oxidation in the fertilized fields.     

生物炭施用下潮土团聚体微生物量碳氮和酶活性的分布特征

  【目的】  探究生物炭配施化肥对不同粒级团聚体中微生物量碳、氮 (MBC、MBN) 含量和胞外酶活性的影响,分析影响团聚体胞外酶活性变化的主控因素,为提升土壤质量提供科学依据。  【方法】  田间微区试验在河南现代农业研究基地进行,供试土壤为石灰性潮土。设置4个处理:不施肥 (CK)、单施化肥 (NPK)、单施生物炭 (BC) 和生物炭配施化肥 (BC+NPK),生物炭是以花生壳为原料高温裂解制备而成,仅在试验开始前施用一次,化肥每季均施用。试验开始于2017年小麦季,于2019年9月玉米收获后采集耕层土壤样品,测定土壤养分含量,分析各粒径团聚体MBC、MBN含量和酶活性。  【结果】  与CK相比,NPK处理可显著提高耕层土壤有效磷、速效钾和硝态氮含量,BC处理可显著提高有机碳和全氮含量,BC+NPK处理则显著提高了以上各指标含量。与CK相比,BC处理显著降低了粒径2～0.25 mm团聚体MBN含量,并明显增加了该粒径的MBC/MBN值;BC+NPK处理显著增加了粒径 > 2 mm和0.25～0.053 mm团聚体中MBC含量 (增幅分别为59.57%和34.68%),也增加了耕层土壤、粒径 > 2 mm和2～0.25 mm团聚体中MBN含量 (增幅分别为17.33%、42.24%和19.28%)。与CK相比,NPK、BC和BC+NPK处理均显著增加粒径 > 2 mm团聚体微生物熵,而BC和BC+NPK处理则显著降低了耕层土壤、粒径2～0.25 mm和0.25～0.053 mm团聚体微生物熵。与CK相比,NPK和BC+NPK处理均显著提高了粒径2～0.25 mm和0.25～0.053 mm团聚体中β-葡糖苷酶、β-纤维二糖苷酶、α-葡糖苷酶和β-木糖苷酶活性;在粒径 > 2 mm团聚体中,仅BC+NPK处理明显提高了该四种酶的活性。与CK相比,NPK和BC+NPK处理均明显提高了粒径 > 2 mm团聚体中脲酶活性及粒径0.25～0.053 mm团聚体中乙酰氨基葡糖苷酶活性,仅BC+NPK处理可显著提高粒径 > 2 mm和0.25～0.053 mm团聚体中亮氨酸氨基肽酶活性。团聚体酶活性变化与MBC、MBN含量以及MBC/MBN值显著相关。粒径 > 2 mm团聚体中酶活性变化与微生物熵、全氮和MBC含量均显著相关,粒径2～0.25 mm团聚体中酶活性变化与MBC/MBN值显著相关,而粒径0.25～0.053 mm团聚体中酶活性变化与MBC含量显著相关。  【结论】  生物炭与化肥配施有利于土壤碳的固存,改善土壤微环境,提升土壤质量,且生物炭添加到土壤中有较长的后效。     

Biochar combined with magnesium fertilizer improves cabbage (Brassica oleraceae L.) yield by modulating physicochemical characteristics and the bacterial community in acidic soil

Biochar and magnesium (Mg) fertilizers have gained increasing attention in agriculture for their potential benefits. However, their combined effects on vegetable growth in southern acidic soils have not been fully elucidated. To address this issue, a study was conducted with six treatments all of which included a baseline amount of compound fertilizer (CK), an increased amount of compound fertilizer (F), Mg fertilizer (M), biochar (BC), biochar combined with Mg (BCM) and an increased amount of compound F with Mg (FM), to investigate their synergistic effects on soil physicochemical properties, enzyme activity, microorganisms and cabbage yield. The results of the study showed that compared with CK, the M, BC and BCM treatments all increased soil organic matter (SOM), exchangeable magnesium (Ex-Mg), acid phosphatase (ACP) and yield, while the F treatment led to a 5% decrease in SOM. The BCM treatment significantly increased SOM, Ex-Mg, ACP by 14%, 40%, and 25%, respectively. The most abundant phyla in BCM processing were Proteobacteria (21%), Chloroflexi (19%) and Actinomycetes (17%); the most abundant genera were Bacillus (8%). Further, the effect of BCM on crop growth promotion was better than that of FM, and the economic analysis showed that compared with FM, the benefit of BCM increased by 14%. Through structural equation modelling analysis, the synergistic increase in yield was because of the increase in soil TP, Ex-Mg, NO₃⁻–N and β-GC caused by BCM, improving soil nutrients and microbial communities, thereby promoting cabbage growth. Therefore, the combined application of biochar and Mg in acidic soil could significantly improve soil fertility and crop productivity, while also reducing the need for costly chemical fertilizers, thereby offering a promising and cost-effective approach for sustainable agriculture.     

不同生物炭对中国亚热带地区湿地松生长、氮肥利用率、土壤N2O和CH4排放以及碳变化的影响

Intensive management of planted forests may result in soil degradation and decline in timber yield with successive rotations.Biochars may be beneficial for plant production,nutrient uptake and greenhouse gas mitigation.Biochar properties vary widely and are known to be highly dependent on feedstocks,but their effects on planted forest ecosystem are elusive.This study investigated the effects of chicken manure biochar,sawdust biochar and their feedstocks on 2-year-old Pinus elliottii growth,fertilizer N use efficiency (NUE),soil N2O and CH4 emissions,and C storage in an acidic forest soil in a subtropical area of China for one year.The soil was mixed with materials in a total of 8 treatments:non-amended control (CK);sawdust at 2.16 kg m-2 (SD);chicken manure at 1.26 kg m-2 (CM);sawdust biochar at 2.4 kg m-2 (SDB);chicken manure biochar at 2.4 kg m-2 (CMB);15N-fertilizer alone (10.23 atom％ 15N) (NF);sawdust biochar at 2.4 kg m-2 plus 15N-fertilizer (SDBN) and chicken manure biochar at 2.4 kg m-2 plus 15N-fertilizer (CMBN).Results showed that the CMB treatment increased P.elliottii net primary production (aboveground biomass plus litterfall) and annual net C fixation (ANCF) by about 180％ and 157％,respectively,while the the SDB treatment had little effect on P.elliottii growth.The 15N stable isotope labelling technique revealed that fertilizer NUE was 22.7％ in CK,25.5％ in the NF treatment,and 37.0％ in the CMB treatment.Chicken manure biochar significantly increased soil pH,total N,total P,total K,available P and available K.Only 2％ of the N in chicken manure biochar was available to the tree.The soil N2O emission and CH4 uptake showed no significant differences among the treatments.The apparent C losses from the SD and CM treatments were 35％ and 61％,respectively;while those from the CMB and SDB treatments were negligible.These demonstrated that it is crucial to consider biochar properties while evaluating their effects on plant growth and C sequestration.     

Effect of long-term fertilization on 15N uptake and retention in soil

We did a pot experiment with three different fertilized soils (no fertilizer (No-F), inorganic fertilizer nitrogen, phosphorus and potassium (NPK), manure plus inorganic fertilizer (MNPK)) from a 19-year fertilizer trial. Three N treatments, (1) no N, (2) 100 mg/kg urea-¹⁵N (N), (3) 50 mg/kg urea-¹⁵N + 50 mg/kg corn straw-N (1/2N + 1/2S), were applied to each soil. The residual soil from the same treatments was used to grow second wheat crop. The MNPK soil had significantly higher nitrogen use efficiency (NUE) in the first growing season, and lower N loss than the NPK, and No-F soils. The 1/2N + 1/2S treatment decreased NUE on each soil, even though the MNPK soil still had highest NUE and lowest N loss. The residual ¹⁵N use efficiency (RNUE) in 1/2N + 1/2S treatment of MNPK soil was higher than NPK and No-F soils. We concluded that long-term application of manure plus inorganic fertilizer increased NUE and decreased N loss.     

生物质炭和秸秆施用对黄褐土生化性质及小麦产量的影响

以位于河南省方城县的黄褐土田间定位试验为平台,监测生物质炭和秸秆连续施用4 a后小麦拔节期和成熟期土壤性质变化及其与成熟期籽粒产量的关系,明确影响小麦产量的主要土壤生化因子。试验包含6个处理,即分别在不施用生物质炭(–B)和施用生物质炭(+B)条件下各设置3个处理:(1)对照(CK),(2)单施化肥(NPK),(3)秸秆还田配施化肥(NPK+S)。结果表明:生物质炭和秸秆施用对土壤生化性质和籽粒产量的影响基本上不存在交互作用。连续4a施用生物质炭后,小麦产量平均降低了17.4%。尽管NPK+S与NPK处理间平均产量没有显著差异,但它们比CK处理产量分别增加了33.8%和37.4%。采用偏最小二乘法路径模型(PLS–PM)分别分析了拔节期和成熟期的土壤速效养分、活性有机质和酶活性对产量的影响,发现小麦拔节期的土壤速效养分含量,特别是氮素的供应是直接影响产量的最为重要因子;而成熟期土壤生化性质对作物产量的影响比较小。因此,为防止黄褐土上施用生物质炭和秸秆后小麦产量降低,需要特别注意小麦拔节期土壤氮素的补充。     

Effects of NPK fertilizer combinations on yield and nitrogen balance in sorghum or pigeonpea on a vertisol in the semi-arid tropics

A long-term experiment was carried out on a Vertisol from 1986 to 1992 to examine the combined effects of NPK fertilizers on yield using sorghum (Sorghum bicolor L. Moench cv. CSH 5) and short-duration pigeonpea (Cajanus cajan L. Millsp. cv. ICPL 87). The fertilizer treatments were as follows: 0 (no fertilization), N (150 kg N ha^-1 ), P (65.5 kg P₂O₅ ha^-1), K (124.5 kg K₂O ha^-1), and all possible combinations (NP, NK, PK, and NPK). In this study we continued this experiment during the period 1993 to 1994 and analyzed the crop yield response to fertilizers and the N balance. The amount of N derived from the atmosphere and fertilizer was estimated by the ¹⁵N natural abundance method and ^l5N isotope dilution method, respectively. A combined application of Nand P fertilizers gave the highest grain yield for the two crops under the 8th and 9th continuous croppings, unlike the application of K fertilizer. The values of total N for the two crops were significantly higher in the NP and NPK plots. These crops took up N mainly from soil. There was a significant positive relationship between the uptake of N_dff and N_dfs by each crop. Pigeonpea or sorghum took up more N from the soil in the N fertilizer plots than in the plots without N, suggesting that soil N fertility was enhanced and the amount of N supplied from soil increased in the plots with consecutive application of N fertilizer for 7 y. Even pigeonpea, which fixes atmospheric N inherently, needed N fertilizer to achieve high grain yield, suggesting that N fixation by the nodules was not always sufficient to meet the N requirements of the crop under these conditions. Although fertilizer N exerted a beneficial effect on plant growth and yield in the two crops, the values of fertilizer N recovery (FNR) by the two crops were considerably low. Therefore, it is suggested that the development of N fertilizer management which could maximize FNR of each crop should be promoted.     

Soil Total Nitrogen and Natural 15Nitrogen in Response to Long-Term Fertilizer Management of a Maize–Wheat Cropping System in Northern China

The Fengqiu long-term field experiment was established to examine effects of organic manure and mineral fertilizers on soil total nitrogen (N) and natural ¹⁵N abundance. Fertilizer regimes include organic manure (OM), one-half N from organic manure plus one-half N from mineral N fertilizer (1/2OMN), mineral fertilizers [N–phosphorus (P)–potassium (K), NP, NK, PK], and a control. Organic manure (OM and 1/2OMN) significantly increased soil total N and δ¹⁵N, which was expected as a great amount of the N applied remained in soils. Mineral NPK fertilizer and mineral NP fertilizer significantly increased total N and slightly increaed δ¹⁵N. Phosphorus-deficient fertilization (NK) and N-deficient fertilization (PK) had no effect on soil total N. Significantly greater δ¹⁵N was observed in the NK treatment as compared to the control, suggesting that considerable N was lost by ammonia (NH₃) voltalization and denitrification in this P-deficiency fertilization regime.