优选白芍醇提工艺比较产地加工对芍药苷含量影响

研究白芍的乙醇回流提取工艺,比较不同加工方法对芍药苷含量的影响。采用正交实验的方法,以芍药苷的含量为指标,考察乙醇浓度、加醇量、提取时间、提取次数4因素。用优选的提取工艺提取白芍,比较不同加工方法对芍药苷含量的影响。结果表明：最佳提取工艺为A1B2C2D2,即50％的乙醇溶剂,8倍量,提取2次,每次2h。未煮未去皮白芍中所含的芍药苷含量最高。结论：优选的工艺稳定可行。为白芍的产地加工提供参考数据。     

牡丹江地区芍药根中主要活性成分的研究与探讨

以牡丹江西安区不同栽培年限的观赏芍药的根,以及同龄生的芍药根的不同径级为试验材料,对其根内几种主要活性成分的含量进行测定,得出芍药根中几种主要初级代谢产物和次生代谢产物的变化趋势。结果表明,不同苗龄栽培芍药根中芍药苷、总黄酮、三萜、多酚含量变化不大。芍药根中初级代谢产物中总糖含量较高,芍药根中总糖、脂肪、灰分的含量随着栽培年限的增长而逐渐增高;根中蛋白质的含量在不同苗龄间差别不大。同龄的芍药根总糖、脂肪、蛋白质、灰分4种营养成分的含量随着根茎变细总的趋势是逐渐减少的。     

紫甘薯花色苷的组分及抗氧化活性研究(英文)

对紫甘薯花色苷的化学成分和抗氧化活性进行了研究.研究采用大孔树脂AB-8纯化紫甘薯花色苷,高效液相色谱-2极管阵列法(HPLC-DAD)分析表明,纯化后的提取物中共含有11种花色苷,其中主要成分为酰化的矢车菊素和芍药素.并测定了紫甘薯总花色苷在DPPH自由基清除体系、超氧阴离子体系、还原力和亚油酸体系的抗氧化活性.在质量浓度均为0.5g/L时,花色苷、L-AA和 BHT的还原力分别为0.572、0.460 和0.121,花色苷的清除DPPH自由基的半数抑制浓度(IC50)和清除超氧阴离子IC50分别为6.94和3.68mg/L,表明花色苷还原能力强,并能有效地清除DPPH自由基和超氧阴离子.此外,紫甘薯花色苷能较好地抑制脂质过氧化.     

HPLC法测定赤芍、返魂草、黄芪、丹参和苍术不同部位的含量

目的:测定赤芍、返魂草、黄芪、丹参和苍术不同部位的含量。方法:采用HPLC法,色谱柱为AGILENT ZORBAX SB-C18(250mm×4.6mm,5μm)。检测芍药苷的流动相为甲醇-0.05mol/L磷酸二氢钾溶液(40:65),波长为230 nm;检测金丝桃苷的流动相为乙腈-0.2%醋酸溶液(18:82),波长为360 nm;检测毛蕊异黄酮葡萄糖苷的流动相为乙腈-水溶液(30:70);波长为260 nm;检测黄芪甲苷的流动相为乙腈-水溶液(32:68),蒸发光检测法;检测丹参酮IIA的流动相为甲醇-水溶液(75:25),波长为270 mn;检测丹酚酸B的流动相为甲醇-乙腈-甲酸-水(30:10:1:59),波长为286nm;检测苍术素的流动相为甲醇-水(79:21),波长为340nm。结果:赤芍中芍药苷的含量符合标准,为合格品;返魂草叶中金丝桃苷比返魂草茎杆含量大,阿多尼弗林碱含量均符合标准,返魂草叶为合格品;黄芪根中毛蕊异黄酮葡萄糖苷和黄芪甲苷含量均符合标准,为合格品,黄芪叶与茎均不合格;丹参中丹参酮IIA与丹酚酸B含量均符合标准,为合格品;苍术中苍术素含量没有达到药典规定的最低含量。结论:本文采用HPLC检测法是常规、简便的检测方法,测定数据准确,为5种中药材的规范化种植及采摘部位奠定了基础。     

伊藤芍药单芽嫁接繁殖试验

分别用剪取的伊藤芍药(Paeonia Lactiflora)枝条最下部第1、2、3个芽嫁接在牡丹实生苗、芍药实生苗、芍药根上,以研究伊藤芍药嫁接成活率。结果表明,用牡丹苗作砧木嫁接成活率最高,芍药苗嫁接次之,芍药根嫁接最低。     

黑龙江产三种芍药不同物候期的芍药甙含量测定

芍药为毛茛科芍药属（Paeonia）植物,野生芍药根作赤芍入药。其活性成分是以芍药甙（Patonifzorin）为主的一类单门类。芍药成具有镇静、解痉、抗炎、抗应激性溃疡病,扩张冠状血管、抗急性心肌缺血及抑制血小板聚集等多种药理作用。黑龙江产芍药原认为有二个种植物,即芍药（P．lactifloraPall）和草芍药（P．obovataMaxim）。刘鸣远氏新近研究,原认为的草芍药实为草芍药和日本芍药（P．japonicaMiyable．et．Takeda）二个种。为了对黑龙江产芍药的药用质量进行科学评价,提供采收、药用、加工的科学依据。我们对黑龙江产的上述三个…     

芍药资源在园林中的应用

芍药作为一种重要的花卉资源,在园林应用中有着非常广阔的前景。本文在讨论了芍药文化、生态习性及种质资源的基础上,提出了芍药专类园、芍药花台、芍药花带、芍药花境和庭院栽植五种园林造景应用形式,并进一步分析了芍药生态性景观、文化艺术性景观、多样性景观和季相景观的4种营建手法。     

论中国牡丹、芍药生产的产业化与可持续发展

对中国牡丹、芍药资源与利用现状进行了概述,指出世界花卉业发展的新趋势与中国加入 WTO为中国牡丹、芍药生产的产业化与可持续发展带来了契机。提出通过迁地保护中心的建立来实现对牡丹、芍药资源利用的可持续性;通过形成具有中国特色的牡丹、芍药新品系与发展产业化生产相配套的技术来实现对牡丹、芍药资源的有效开发与利用;通过专业人才的培养、信息网络的完善来为牡丹、芍药产业的发展注入活力;通过优化产业结构,加强宏观调控来保证牡丹、芍药产业的健康发展,从而实现中国牡丹、芍药生产的产业化与可持续发展。     

日本芍药引种栽培试验

芍药（Paeonia lactiflora．pall）是我国栽培历史悠久的传统名花之一，与牡丹并称为“花中二绝”，属芍药科芍药属宿根植物。芍药花朵硕大，富丽堂皇，花色、花型丰富，可与花王牡丹媲美，被称为“花相”。近几年随着切花市场的发展，芍药由于花大美丽且花枝硬长宜作切花，越来越受到人们的青睐。2001年我们从日本引进4个品种的芍药，丰富了菏泽的芍药品种，填补了芍药生产中的一些空白。例如早花品种2号，它的花期在4月下旬，和晚花牡丹相接应，且花大如牡丹，花期长，使牡丹花期与芍药花期连在了一起，大大延长了菏泽观花旅游的时间。     

牡丹芍药野生种迁地保护收效显著 豫西资源圃七年间引种驯化水平跃居国际前列

经过7年多的不懈努力,河南豫西山区芍药属野生种迁地保护资源圃已从西藏、云南、四川、甘肃等地收集到野生牡丹、芍药达14种。其中牡丹组9个种全部原产中国,已基本收集齐全。芍药组原产中国的9个种,现已收集5个。牡丹组的部分种和芍药组的芍药、草芍药、川赤芍3个种,迁地保护引种驯化水平居国际前列。     

Paeoniflorin alleviates liver fibrosis by inhibiting HIF-1α through mTOR-dependent pathway

HIF-1α/mTOR signaling pathway is considered to play a crucial role in genesis and progress of tissue fibrosis. The elevation of HIF-1α and mTOR is relevant to CCl4 induced liver fibrotic rats. Paeoniflorin has been consistently shown to exhibit multiple pharmacological effects in liver disease. However, so far, no research demonstrates the relationship between paeoniflorin and HIF-1α/mTOR fibrogenesis pathway in liver fibrosis. In this study, the liver fibrosis was performed by CCl4 rats and HSC-T6 cell line. The data demonstrated that paeoniflorin treatment could attenuate liver fibrosis and inhibit the activation of HSC. Moreover, paeoniflorin significantly enhanced hepatic function by decreasing serum level of ALT, AST and ALP, and increasing level of ALB, TP. Meanwhile, ECM degradation was modulated by paeoniflorin treated rats with a remarkable reduce of α-SMA and collagen III mRNA expression. Moreover, the alleviation effect of liver fibrosis was relevant to inhibiting HIF-1α and phosphor-mTOR. Our data indicate that paeoniflorin alleviates liver fibrosis by inhibiting HIF-1α expression partly through mTOR pathway and paeoniflorin may be a potential therapeutic agent for liver fibrosis.     

Monoterpene glycosides from Paeonia delavayi

A new monoterpene glycoside, 4-O-methyl-4'-hydroxy-3'-methoxy-paeoniflorin (1), was isolated from the root cortex of Paeonia delavayi along with the known paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, albiflorin and a paeonilactone-A.     

New monoterpene glycosides from Paeonia lactiflora

Three new monoterpene glycosides named 4-O-methyl-paeoniflorin (1), isopaeoniflorin (2), and isobenzoylpaeoniflorin (3), together with two known monoterpene glycosides, paeoniflorin (4) and benzoylpaeoniflorin (5), were isolated from the roots of Paeonia lactiflora. Their structures were established on the basis of spectral and chemical evidence.     

Comparative pharmacokinetic study of paeoniflorin and albiflorin after oral administration of Radix Paeoniae Rubra in normal rats and the acute cholestasis hepatitis rats

A comparative study was designed and conducted to compare the pharmacokinetic difference of paeoniflorin and albiflorin after oral administration of Radix Paeoniae Rubra to normal rats and the acute cholestasis hepatitis rats induced by alpha-naphthylisothiocyanate (ANIT). UPLC-ESI-MS/MS method was employed to determine the level of paeoniflorin and albiflorin in rat plasma using geniposide as the internal standard (IS). Unpaired Student's t-test was used for the statistical comparison. The investigation showed that there were significant differences between the normal rats and the acute cholestasis hepatitis rat groups in calculated parameters, such as AUC(0-t), AUC(0-∞), T(max) and CLz/F. The results indicated that acute liver injury in rats could alter the pharmacokinetics of drug. Since patients are the final users of the drug, it is essential to investigate the pharmacokinetics of the drug in disease status. Therefore, we used normal rats and the acute cholestasis hepatitis rats to study pharmacokinetics of Radix Paeoniae Rubra with the purpose of examining the influence of disease on the metabolic course.     

蓖麻枝叶形态特征对蒴果性状的影响

为给蓖麻高产栽培提供参考,以‘淄蓖5号’为材料,测定蓖麻枝叶形态特征指标(开花时叶面积、灌浆期叶面积、收获时分枝直径)和蒴果性状指标(每穗果球数、果球质量、每果球种子数、种子质量、穗长、穗宽),分析蓖麻枝叶形态特征对蒴果性状的影响。相关性分析结果表明,按蓖麻枝叶形态特征的影响由大到小排序,各蒴果性状指标依次为果球质量、每穗果球数、种子质量、每果球种子数、穗长、穗宽。回归分析结果表明,蓖麻分枝直径对每穗果球数、果球质量变异的解释程度分别为31%、37%;开花时叶面积对每穗果球数、每果球种子数变异的解释程度分别为53%、51%;灌浆期叶面积对种子质量变异的解释程度为51%;开花时叶面积、灌浆期叶面积和收获时分枝直径对每穗果球数、果球质量、每果球种子数、种子质量变异的解释程度分别为67%、68%、76%、79%。修枝时保留粗壮枝条有利于获得更多果球、更大果球质量,开花时保持较大的叶片面积是获得更多果球和种子的重要保障,保持灌浆期较大叶面积利于获得饱满的种子。     

The impact of flower density and irrigation on capsule and seed set in <Emphasis Type="Italic">Eucalyptus globulus</Emphasis> seed orchards

Low capsule set is a major factor limiting the productivity of Eucalyptus globulus seed orchards. This study tested the effect of flower density, as well as two common irrigation techniques on capsule and seed set. Ramets with high flower density had significantly lower capsule set (69.7%) than those with low flower density (81.7%). In a regulated deficit irrigation trial, the non-irrigated ramets set a higher proportion of capsules (63.6%) than the ramets that received conventional irrigation (CI) (51.4%). In a partial root zone drying (PRD) trial, capsule set was highest in the absence of irrigation (74.7%) followed by the PRD treatment (67.8%) and then CI (53.7%). The CI treatment tended to produce the highest number of seed per capsule. Increased water availability resulted in increased vegetative growth, which was associated with higher levels of abortion in developing capsules but those surviving tended to have higher seed set. It is argued that the observed effects of irrigation and flower density can be explained by resource competition between vegetative and reproductive growth as well as competition among reproductive structures themselves.     

香榧细小卷蛾的生物学特性及其防治

通过对香榧细小卷蛾幼虫头壳宽度的度量和数学分析,发现其幼虫的头壳宽度为不连续分布,呈现4个较为集中的分布区间,可以以此来确定幼虫的龄级.并描述了香榧细小卷蛾各虫态的形态特征、生活史及生活习性,提出了物候法预测发生期的预测预报方法和防治建议.     

山楂种子抑制物质的提取分离及生物测定

为了更深入地了解山楂种子的休眠机理,以野生山楂种子为试材,用80%的甲醇溶液对其内果皮、种皮和种胚进行浸提,然后测定提取物对白菜种子发芽的影响;将浸提物分离成A、B、C、D、E、F、G共7相,浓缩后分别测定其对白菜种子发芽的影响。结果表明,山楂种子的内果皮、种皮、种胚均含有发芽抑制物质,其抑制作用依次减弱。7个分离相中除了种皮的A相及内果皮的G相对白菜种子发芽没有影响外,其余各相均显著抑制发芽。