NaCl胁迫下玉米地方品种的种子萌发效应

采用室内培养和检测,以5个玉米地方品种为材料,设置高盐(NaCl)和自来水2种处理,研究不同玉米地方品种在盐胁迫下的种子萌发和生理特性变化.结果表明,盐胁迫对玉米地方品种的种子萌发和生理有不同程度的影响,不同玉米地方品种的耐盐性存在明显的基因型差异.总体而言,不同地方品种的同一形态性状和同一地方品种的不同形态性状的耐盐胁迫指数存在差异.综合评价盐胁迫下5个玉米地方品种的耐盐特性,"DP44"是耐盐性强的品种,可作为耐盐种质加以选择,而"DP21"的耐盐胁迫能力较差.此外,对玉米品种进行耐盐胁迫的遗传改良是可行的.     

高温胁迫对早稻根系质膜ATPase活性及NH4+吸收速率的影响

以耐热型品种“农大228”、“082”和热敏感品种“茉莉占”、“协清早B”为材料，研究高温胁迫后不同耐性品种之间的生理差异。结果如下：高温胁迫后，耐热型品种根系质膜ATPa~e活性升高，热敏感型品种活性降低；耐热型品种根系NHf吸收速率不受影响，热敏感型品种明显下降。高温胁迫后根系NHf吸收动力学参数的变化与品种的耐热性无关。     

营养液pH变化对刺梨苗吸收硝态氮和铵态氮的影响

研究介质pH变化条件下刺梨(Rosa roxburghii)对不同形态氮素吸收特性的影响,能为不同pH土壤上刺梨的合理施肥提供科学依据。本研究以‘贵农5号’刺梨实生苗为材料,采用营养液培养和离子耗竭法,在分别供给硝态氮和铵态氮的条件下,设置营养液分别为pH 4、5、6、7、8、9的6个处理,测定不同pH营养液培养的刺梨苗对NO₃^-、NH₄⁺的吸收动力学参数和刺梨苗的植株高度、干重生物量、氮含量及氮吸收量,分析营养液pH变化与NO₃^-、NH₄⁺的吸收动力学参数的相关性和营养液pH变化与刺梨苗植株高度、生物量、氮含量和氮素吸收量的相关性。研究结果表明,刺梨根系对硝态氮、铵态氮及总氮的吸收规律均符合Michaelis-Menten酶动力学方程。营养液的pH变化能够改变刺梨苗根系对NO₃^-和NH₄⁺的吸收特性。在营养液pH 4~9的范围内和供给硝态氮的条件下,刺梨实生苗的根系对NO₃^-的最大吸收速率(I_max)、根系与NO₃^-的亲和力、NO₃^-流入根系的速率(α)、植株高度、干重生物量、氮含量及氮的吸收量随营养液pH的增大而明显降低,营养液的pH变化与上述指标呈极显著负相关。在供给铵态氮的条件下,刺梨实生苗根系对NH₄⁺的上述指标随营养液pH的升高而明显增大,营养液的pH变化与上述指标呈极显著正相关。在酸性条件下有利于刺梨苗对硝态氮的吸收,升高营养液的pH不利于刺梨苗吸收硝态氮。碱性条件有利于刺梨苗对铵态氮的吸收,酸性条件对刺梨苗吸收铵态氮有不利影响。     

盐碱胁迫对小麦幼苗根系活力和苯丙氨酸解氨酶活性的影响

分别采用两种中性盐(NaCl和Na2SO4)和两种碱性盐(NaHCO3和Na2CO3)混合模拟不同浓度的盐碱胁迫条件,对龙麦26和克旱16两种不同基因型春小麦幼苗进行7d胁迫处理。分别测定叶片中苯丙氨酸解氨酶活性和根系活力,以探讨盐、碱两种胁迫下小麦根系对离子交换吸附能力的影响。结果表明:盐碱混合胁迫增强了小麦叶片的苯丙氨酸解氨酶活性,增加了根系活跃吸收面积,增大了根系活力。这3个指标受胁迫溶液离子浓度、pH值、碱性盐比例等多重影响,且品种间存在一定差异性。     

耐盐性不同水稻品种根系对盐胁迫的响应差异及其机理研究

为阐明耐盐性不同的水稻品种根系和产量对盐胁迫的响应及其生理特性,以4个耐盐水稻品种和2个盐敏感水稻品种为材料,设置4个不同的盐浓度处理(0.0、1.0、2.0和2.5g/kg)。结果表明,与盐敏感水稻品种相比,耐盐水稻品种能够耐受更高浓度的盐胁迫(2.5g/kg),且减产幅度较小。耐盐水稻品种具有较高的产量,得益于其较高的总颖花量和结实率,且总颖花量对产量的影响最大。与盐敏感水稻品种相比,在分蘖中期、穗分化期和抽穗后15d,耐盐水稻品种在盐胁迫下具有更大的根系总吸收表面积和活跃吸收表面积,更高的根系氧化力,更有优势的根长、根直径和根表面积,较低的过氧化氢含量,较高的脱落酸含量以及更强的固定Na⁺的能力。上述结果表明,分蘖中期、穗分化期和抽穗后15d根系生理性状和形态性状差异会影响水稻产量构成因素中的总颖花量和结实率,进而影响产量,且根干重与根长对总颖花量和结实率的影响最大。这也证实了较发达的根系是水稻具有较强耐盐性的生理基础。耐盐水稻良好的根系生理和形态性状是获得高产的基础。该研究结果对水稻耐盐生理机制研究与水稻耐盐育种有借鉴意义。     

盐胁迫条件下甜高梁幼苗的光合特性及光系统Ⅱ功能调节

通过气体交换和叶绿素荧光猝灭动力学研究了盐胁迫对甜高粱幼苗碳同化能力和光系统Ⅱ光化学效率的影响。结果表明，50和100mmol L^-1的盐（NaO）处理对叶绿素含量、相对含水量和膜质过氧化程度影响很小；200mmol L^-1 NaCl处理导致叶绿素含量和相对含水量明显下降、膜质过氧化程度增加。50mmol L^-1 NaCl处理未影响甜高粱幼苗的净光合速率；NaCl浓度大于50mmol L^-1时，净光合速率开始迅速降低；同时，气孔限制值（Ls）也减小；而且，光合能力的下降未能通过增加CO2浓度得以恢复。甜高粱幼苗的初始荧光（Fo）、最大荧光产量（Fm）和最大光化学效率（Fv/Fm）只在200mmol L^-1 NaCl处理时有较大程度的下降。此外，50mmol L^-1 NaCl胁迫也没有影响甜高粱幼苗的荧光猝灭动力学参数；当NaCl浓度大于50mmol L^-1时，光系统Ⅱ开放反应中心转化效率（Fv，F’m），光化学猝灭系数（qp）和光系统Ⅱ实际光化学效率（ΦPSⅡ）开始下降，而非光化学猝灭（NPQ）提高。因此认为，盐胁迫导致的碳同化能力的降低属于非气孔限制；碳同化能力的降低改变了甜高粱光系统Ⅱ的激发能利用和分配。100mmol L^-1盐胁迫条件下，甜高粱幼苗主要通过增加热耗散来消耗过多的激发能，而200mmol L^-1盐胁迫条件下通过减少光能吸收和增加热耗散来维持光能捕获和利用的平衡。     

褪黑素对盐胁迫下高粱种子萌发的缓解效应及生理机制

为了探究外源褪黑素影响高粱种子耐盐性的机理，本研究以粒用高粱品种晋杂2002的种子为试验材料，分析盐胁迫下褪黑素浸种对高粱种子萌发及生理特性的影响。结果表明，盐胁迫能明显抑制高粱种子萌发，增加相对电导率及丙二醛含量，使其干物质积累量减少；适宜浓度褪黑素浸种能缓解盐胁迫对高粱种子萌发的抑制作用。褪黑素浸种可通过提高高粱种子萌发过程中抗氧化酶活性，清除过量活性氧积累，减轻膜脂过氧化程度；增加渗透调节物质含量，维持细胞膨压，提高吸水能力；同时还可增加种子胚根根系活力，提高胚芽绿色部分光合色素含量，这些变化共同提高了高粱种子萌发期抵御盐胁迫的能力，促进其种子萌发及干物质积累；通过主成分分析法筛选出胚芽长度和可溶性糖含量两个指标可作为褪黑素缓解盐胁迫下高粱种子萌发的评价指标。     

盐胁迫对油桃生理生化特性的影响

为分析不同油桃的耐盐特性,为后续的耐盐品种选育研究打下基础,以‘华光’油桃盆栽苗为试材,研究不同程度盐胁迫对植株茎生理指标的影响。试验发现0.4%盐胁迫下各指标与对照组无明显差异,0.8%盐胁迫中后期开始有一定影响,而1.2%盐胁迫初期时相关指标便有极明显差异,表明该盐环境为植株能够承受的极限。在此基础上,以‘中油桃4 号’、‘艳光’、‘华光’、‘千年红’、‘玫瑰红’5 个品种油桃盆栽苗为试验材料,分析了1.2%盐溶液胁迫下的生理指标变化,研究不同品种油桃的耐盐特性。结果表明：不同油桃品种在盐胁迫下的表现差异性很大,黄肉品种‘中油桃4 号’耐盐特性优于‘千年红’;白肉品种‘华光’耐盐性强于‘艳光’,‘玫瑰红’高于‘华光’;白肉品种耐盐特性整体上强于黄肉品种。     

植物响应盐碱胁迫的机制

土壤盐碱化对农林业生产和发展的影响日渐严重,已经成为全球范围内所面临的重大环境问题。研究盐碱胁迫的危害以及植物对盐碱胁迫的响应机制,有助于挖掘植物耐盐碱基因,选育耐盐碱品种,改良盐碱地,提高农作物的产量,扩大园林植物的栽培应用。盐、碱实际上是两种不同的非生物胁迫,碱胁迫在盐胁迫的基础上还增加了高pH胁迫,其危害程度较盐胁迫更深。本综述分析了土壤盐渍化现状,盐碱胁迫对植物产生的渗透胁迫、离子毒害、高pH伤害、活性氧胁迫等危害,从渗透调节物质的合成、离子的吸收转运与pH调节、增强抗氧化酶活性及内源激素响应等方面阐述了植物耐盐碱的生理机制;从盐碱胁迫的信号转导、转录因子调控响应及抗盐碱相关基因的表达等方面梳理了植物耐盐碱的分子机制。最后对植物适应盐碱胁迫的研究方向及多组学联合分析在全面研究植物抗盐碱机制中的应用作出了展望。     

盐胁迫对不同小麦品种种子萌发的影响

不同浓度ＮａＣｌ胁迫对不同小麦品种种子萌发过程的影响试验结果表明，在盐胁迫下种子发芽率、发芽指数、活力指数和种子内α－淀粉酶活性下降；胚芽渗透势降低；种子内储藏物质转化速度变慢。这些反应均随盐胁迫强度的提高而增强。α－淀粉酶活性与种子萌发活力指数、种子内储藏物质的转化速度等均呈显著正相关。不同品种对盐胁迫反应不同，耐盐品种可维持较高的α－淀粉酶活性，种子内储藏物质转化速度快，具有较高水平的萌发活力     

小麦新鲜根系铁氰化钾还原酶与氢、钾跨膜运输的关系

为研究麦根质膜氧化还原反应与钾离子吸收的关系,比较不同基因型小麦吸钾差异,以水培和短期生物吸收试验方法,检测到小麦根系铁氰化钾还原酶的存在,而且不同品种铁氰化钾还原酶还原活性不同。反应液中由于根系内外质子和钾离子的迁移,加与不加铁氰化钾,不同品种根系都引起了溶液pH的改变,但外部电子受体Fe(CN)63-的存在即铁氰化钾还原酶底物的存在加强了溶液pH改变（更多为降低）的程度。铁氰化钾还原也明显影响了根系钾离子的吸收或外排动态,但不同品种影响不同。小麦根细胞膜氧化还原系统明显影响质子和钾离子的跨膜运输,不同品种影响不同。     

剑河县土壤微量元素与有机质、pH值的关系研究

对剑河县12乡镇72个土壤样本的有效态微量元素、有机质及pH值进行了测定,研究了土壤中有效锌、有效铜、有效锰、有效铁、有效硼元素与有机质、pH值的关系.研究结果表明：土壤有机质与有效铁、有效铜、有效锌、有效硼含量有正相关性,与有效锰含量呈负相关性;土壤pH值与有效锰、有效铁、有效锌、有效硼含量有负相关性,与有效铜含量呈正相关性的规律.在生产过程中,应针对不同乡镇的土壤微量元素状况,应在长期施用氮磷钾肥料的基础上,可采取调节有机质和pH值等提高微量元素有效性的方法来提高农作物对微量元素的吸收,从而提高农作物产量、质量.     

污灌区盐渍化土壤重金属Cd的形态分析及其影响因素

以受盐渍化及重金属Cd污染的天津污灌区土壤作为研究对象,探讨盐渍化土壤重金属的形态分布及其影响因素。研究的盐分种类主要为NaCl和Na_2SO_4,盐度7个[添加质量分数依次为0%(CK)、0.2%、0.4%、0.6%、1%、2%和5%],采用Tessier连续提取法测定土壤前3种重金属的形态(可交换态、碳酸盐结合态、铁锰氧化态),得出重金属的形态分布规律。结果表明:在添加NaCl盐度条件下,土壤中Cd碳酸盐结合态可交换态铁锰氧化态,可交换态含量与盐度、重金属总量、pH值和有机质均呈显著相关。在添加Na_2SO_4盐度条件下,土壤中Cd铁锰氧化态可交换态碳酸盐结合态,可交换态、碳酸盐结合态与pH值和有机质均呈极显著相关,铁锰氧化态与pH值呈显著负相关。盐度与Cd各形态也有不同程度的相关性。在添加NaCl盐度条件下,pH值对可交换态含量有重要影响;有机质对碳酸盐结合态含量有重要影响;Cd含量对铁锰氧化态含量有重要影响。在添加Na_2SO_4盐度条件下,有机质对可交换态含量有重要影响;pH值、有机质对碳酸盐结合态含量有重要影响;CEC对铁锰氧化态含量有重要影响。得出的Cd形态分布规律以及土壤各理化性质对Cd形态含量的影响分析对土壤重金属的污染防治和生态风险评价提供了基本的理论依据。     

北京果园土壤营养状况和微生物种群调查分析

为了探明北京地区主要果树的肥力水平,应用田间土壤取样方法,对苹果、梨、桃和葡萄三种不同产量品质水平(高、中、低)果园的土壤理化性状、营养水平和微生物种群数量进行分析。结果表明：土壤容重1.06～1.60 g.cm-3,不同树种差异不显著;土壤阳离子交换量(CEC)81.47～238.83 mmol.kg-1,苹果、桃、葡萄园显著高于梨园,同一树种的高(H)、中(M)型果园均高于低(L)型园;土壤pH在7.39～8.55间;有机质含量7.34～19.50 g.kg-1,不同树种差异不显著,同一树种的H园高于M、L园;全氮0.41～1.09 g.kg-1,苹果、桃和葡萄园显著高于梨园,同一树种H园高于M、L园;速效磷14.54～185.50 mg.kg-1,速效钾99.77～267.50 mg.kg-1,不同树种及不同类型果园间差异均不显著;有效铁、锌和硼含量分别为4.78～33.42mg.kg-1、0.97～10.54mg.kg-1、0.19～0.58mg.kg-1。四种果园的土壤微生物总量为0.45×107～1.23×107cfu.g-1,苹果园最高,梨园最低;细菌数量占绝对优势,放线菌次之,真菌最少。不同树种果园土壤营养水平(Pi)顺序为苹果(0.71)＞葡萄(0.58)＞桃(0.54)＞梨(0.31),同一树种为H＞M＞L。综合分析,北京果园土壤营养和微生物种群总体状况为土壤pH偏高,有机质和全氮缺乏,磷钾比偏高,有效硼不足,土壤微生物总量偏少等,为北京果园土壤改良提供了基本理论依据。     

Ionic Homeostasis and Expression of Na+ Related Genes of Cotton under Different Salt and Alkali Stresses

[Objective] Maintaining intracellular ion homeostasis is one of the important salt-tolerant mechanisms of crops. This study aims to analyze differences in response characteristics of cotton ionome and salt-tolerant gene expression under different saline-alkali stresses, which provides a basis for understanding the mechanism of salt tolerance and improving salt tolerance of cotton. [Method] Using Lumianyan 24 as the experimental material, three kinds of salt and alkali stress types (salt stress, alkali stress, and mixed salt-alkali stress) and two concentration gradients (low and high concentrations) were set under pot cultivation conditions. Meanwhile, non-saline-alkali stress treatment was set as control. The dry matter weight of cotton plants and root morphological parameters including root length, root surface area, and root volume were measured in this study. The concentrations of 13 elements such as P, Na, K, Ca and Mg in different organs of cotton plants were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). The relative expressions of salt tolerance related genes GhDFR1, GhSOS1, GhNHX1 and GhAKT1 were determined by the quantitative real-time polymerase chain reaction method. [Result] 1) Salt and alkali stresses significantly inhibited cotton growth. The growth inhibition rate of cotton under mixed salt-alkali stress treatments (48.7%–57.9%) was significantly higher than that under salt stress (27.6%–49.9%) and alkali stress (21.2%–35.5%) treatments. Under salt stress and mixed salt-alkali stress treatments, both shoot and root growth of cotton were significantly inhibited, dry matter weight, root length, root surface area and root volume were significantly reduced, while root growth was less inhibited under alkali stress treatments. 2) Under three types of saline-alkali stresses, Na content and Mo content in different organs of cotton plant increased significantly, but N content in leaves and roots decreased. 3) Under salt stress treatments, the uptake of Ca, Mg, Fe, Mn and Zn in cotton was inhibited, and the ion balance was maintained by promoting the transport of these ions and P, K. 4) In addition to Ca, Mg, Fe, Mn and Zn, P uptake was also inhibited under alkali stress treatments, but K uptake and P, K, Ca, Mg, Fe, Mn and Zn transport were promoted. 5) Under mixed salt-alkali stress treatments, especially under high salinity and pH conditions, most of nutrients uptake was inhibited, and the transport capacity of Ca, Mg, Zn, Mn and Fe was reduced. 6) The relative expression of GhSOS1 and GhAKT1 genes increased significantly under salt stress treatments, but increased first and then decreased under alkali stress and mixed salt-alkali stress treatments. The relative expression levels of GhSOS1 and GhAKT1 genes under three types of saline-alkali stresses were alkali＞salt＞mixed saline-alkali stress. With the increase of soil salinity and pH value, the relative expression of GhNHX1 gene increased first and then decreased. The expression levels of GhNHX1 gene were salt＞alkali＞mixed salt-alkali stress. [Conclusion] Due to high salinity and pH value, mixed salt-alkali stress significantly inhibits cotton growth and ions uptake, which restricts the transport of P, K, Ca, Mg, Zn, Mn and Fe. The decrease of K and Na regulation ability leads to ions imbalance.     

石灰土壤中植物铁营养生理生化的研究进展

为了阐明石灰土中植物缺铁失绿的生理生化机理,综述了石灰土或重碳酸盐胁迫下植物对铁吸收和转运的研究进展。在石灰土壤中,由于存在CaCO3或MgCO3,它们与CO2和H2O反应形成过量的碳酸氢根离子（也叫重碳酸盐）,从而导致了石灰土中富含重碳酸盐。总结了重碳酸盐改变土壤pH,降低了土壤中铁的可利用性,从而限制了植物对土壤中铁的吸收;另一方面,植物在重碳酸盐胁迫下,诱导植物根系产生一系列的生理生化反应响应低铁生境,包括植物根系分泌相关的物质或质子到根际土壤酸化土壤,诱导根系铁还原酶基因表达从而增强铁还原酶活性,以及诱导铁转运体基因的表达等。石灰土壤中的存在大量的重碳酸盐,针对该土壤环境的特殊性,未来应加强以下几个方面的研究,（1）重点研究重碳酸盐对铁在植物组织之间的长距离运输的影响,挖掘重碳盐影响铁转运的分子机制;（2）植物细胞内的铁蛋白是否受到重碳酸盐的影响,仍然需要开展相关的研究;（3）进一步研究土壤中的微生物与重碳酸盐在植物胁迫中的作用。     

Effects of Soil Treatment with Iron, Manganese and Zinc on Growth and Micronutrient Uptake of Sunflower Plants Grown in High-pH Soil

The purpose of this work was to study the effects of adding different concentrations of iron (Fe), manganese (Mn) and zinc (Zn) in irrigation water to high-pH sandy soil on the growth and micronutrient uptake of sunflower plants ( Helianthus annus L., cv. Giza 1). The experiment was carried out in Mitscherlish pots under greenhouse conditions. Low Fe, Mn and Zn concentrations were found to have a positive effect on the micronutrient contents of the plant organs. The number of leaves and the leaf area were increased by addition of Fe, Mn and Zn. Root size was increased by addition of Fe and Mn. Stem and root lengths were increased by Mn only. It could be concluded that continuous addition of Fe, Mn and Zn in irrigation water to high-pH sandy soil deficient in these nutrients, where root growth was contained within a limited volume (in pots), led to increases in growth and micronutrient uptake (Fe, Mn and Zn). Further studies are needed to determine whether similar effects will be found under field conditions.     

Segregation of peach and nectarine (Prunus persica (L.) Batsch) cultivars according to their organoleptic characteristics

Cultivar segregation according to the sensory perception of their organoleptic characteristics was attempted by using trained panel data evaluated by principal component analysis of four sources per cultivar of 23 peach and 26 nectarine cultivars as a part of our program to develop minimum quality indexes. Fruit source significantly affected cultivar ripe soluble solids concentration (RSSC) and ripe titratable acidity (RTA), but it did not significantly affect sensory perception of peach or nectarine flavor intensity, sourness or aroma by the trained panel. For five out of the 49 cultivars tested, source played a role in perception of sweetness. In all of these cases when a source of a specific cultivar was not classified in the proposed organoleptic group it could be explained by the fruit having been harvested outside of the commercial physiological maturity (immature or over-mature) for that cultivar. The perception of the four sensory attributes (sweetness, sourness, peach or nectarine flavor intensity, peach or nectarine aroma intensity) was analyzed by using the three principal components, which accounted for 92 and 94% of the variation in the sensory attributes of the tested cultivars for peach and nectarine, respectively. Season did not significantly affect the classification of one cultivar that was evaluated during these two seasons. By plotting organoleptic characteristics in PC1 and PC2 (∼76%) for peach and nectarine, cultivars were segregated into groups (balanced, tart, sweet, peach or nectarine aroma and/or peach or nectarine flavor intensity) with similar sensory attributes; nectarines were classified into five groups and peaches into four groups. Based on this information, we recommend that cultivars should be classified in organoleptic groups and development of a minimum quality index should be attempted within each organoleptic group rather than proposing a generic minimum quality index based on the ripe soluble solids concentration (RSSC). This organoleptic cultivar classification will help to match ethnic preferences and enhance current promotion and marketing programs.     

广元植烟区土壤养分空间分布特征研究

旨在明确广元植烟区土壤pH和土壤养分的空间分布特征,土壤养分与pH的相关性,为广元地区烤烟种植合理施肥提供科学依据。2013—2014年,通过网格法布点采集、检测、分析广元地区846个土壤样本。结果表明：广元烟区土壤pH平均为7.68±0.7,变异系数9.06%,pH 7.5~8.5的土壤样本占总样本数的69.53%。有效磷、交换性钙离子、有效铁、有效锌和有效硼的变异系数在50%~100%之间,接近强变异性;有效铜和有效硫的变异系数大于100%,其中有效硫变异系数最大,其值107.12%,有效锰最小,变异系数22.51%;土壤有效硫、交换钙离子和交换性镁离子与土壤pH呈著正相关,相关系数分别是0.31^*、0.51^*和0.14^*。由此可知,广元植烟土壤养分含量地域间分布不均衡,土壤pH总体偏碱,土壤中有机质和有效硼总体缺乏,碱解氮和速效钾含量总体偏低,有效磷和有效锌适中;有效锰、有效铁、有效铜、有效硫和交换性镁离子总体丰富,交换性离子钙总体极丰富。     

Efficacy of HBED/Fe3+ at supplying iron to Prunus persica in calcareous soils

To date, the application of synthetic Fe chelates has been the most effective solution to correct Fe chlorosis. Commercial products based on the EDDHA/Fe³⁺ (ferric ethylenediamine-N,N′-bis(hydroxyphenylacetate)) are the most effective and commonly used; however, their synthesis produces products with a low purity (∼6% chelated Fe).The aims of this work were to study the effectiveness of 2 Fe chelates of higher purity, HJB (N,N′-bis(2-hydroxy-5-methylbenzyl)ethylenediamine-N,N′-diacetic acid) and HBED (N,N′-bis(2-hydroxybenzyl)ethylenediamine-N,N′-diacetic acid), to prevent and correct the Fe deficiency in nectarine and flat peach trees, respectively, grown in alkaline field conditions and to compare their effectiveness with the traditional EDDHA. Tree status was evaluated during 2 growing seasons, and nutritional parameters related to the Fe concentration in leaves and flowers, the SPAD index and micronutrient ratios were evaluated. Moreover, the yield and the size of fruit were evaluated in the experiment with nectarine trees.Parameters such as the SPAD index, the Fe concentration and the Fe/Mn ratio in leaves showed that EDDHA/Fe³⁺, HJB/Fe³⁺ and HBED/Fe³⁺, especially at low doses, presented a similar ability to prevent chlorosis symptoms in nectarine trees.Chlorotic flat peach trees treated with commercial EDDHA/Fe³⁺ showed a faster regreening (SPAD index) during the first year of chelate application; however, no significant differences among treated trees could be observed at the end of the second growing season. The results of measuring Fe concentration in leaves were not as conclusive; however, flower analysis after the treatment application showed a good recovery in treated trees.These studies show the efficacy of chelates with a purer composition than the traditional EDDHA/Fe³⁺ to correct and prevent Fe deficiency.