Water circulation, groundwater outflow and nutrient dynamics in Mida creek, Kenya

The relationship between physical hydrodynamic processes and nutrients dynamics was investigated in Mida creek, a groundwater influenced mangrovefringed creek in Kenya between March 1996 and May 1997. The research involved spot and timeseries measurement of nitrate–nitrite, ammonia, silicates, phosphates, salinity, temperature, sealevel as well as tidal currents at seven stations located in the front, middle and backwater zones of the creek. Groundwater level as well as total dissolved solids' concentration, salinity, temperature and nutrients' concentration were also measured once every month in shallow wells (watertable<5m) located in the upper region of the creek. Results of the study show that nutrient concentrations vary with the tide and that, though there is no river drainage, they are of the same magnitude as in mangrove creeks with substantial river runoff. The peak concentrations of NH ₄ ⁺ –N (5.45M), NO ₂ –NO ₃ (5.63M), PO ₄ ^3– –P (0.58M) and SiO ₃ ^2– –Si (81.36M) in the creek occurred during flood tide, 2–3h before high waters. The (NO ₂ ^– + NO ₃ ^– )–N concentrations declined rapidly during ebb tide, reaching the minimum levels during low water. Contribution of groundwater seepage to the net nutrients flux (particularly on nitrite–nitrates) is largest in dry seasons. The study shows that groundwater outflow sustains the mangroves during periods of severe salinity stress and nutrients deficiency in dry seasons. This is essentially by limiting salinity increase and by boosting nutrient supply in dry seasons.     

Cultural factors influencing adventitious shoot and plantlet formation from slash pine cotyledons

Cultural factors affecting in vitro shoot and subsequent plantlet formation of slash pine (Pinus elliotti Engelm.) cotyledons were investigated. Basal media composition, N⁶-benzylaminopurine (BAP) concentration and exposure time significantly influenced bud induction in cotyledons cultured under a continuous photoperiod of 35–40 mol m^–2 s^–1 at 24 ± 1 °C. The largest number of adventitious shoots was obtained after 28 days exposure to 66 M BAP-supplemented modified Gresshoff and Doy 1 (GD1) medium. Relatively high frequencies of large shoots were obtained after a 14-day exposure to 22 M BAP-supplemented Brown and Lawrence (BL) or 66 M BAP-supplemented GD1. Adventitious shoots derived from 21- or 28-day exposures to BAP developed more slowly and were smaller in size than those derived from a 14-day exposure to the cytokinin. Shoot differentiation and subsequent growth were also influenced by basal media, media concentration, and presence of activated charcoal in the medium. The percentage of cotyledons forming shoots was highest on half-strength GD1 medium containing activated charcoal. Rooting was achieved in vitro under a continuous photoperiod of 60–70 mol M^–2 S^–1. Roots were formed when excised shoots were planted on GD 1/2 medium supplemented with 2.68 M 1^–1 a-napthaleneacetic acid (NAA) with or without BAP for 14 days. The proposed technique of slash pine propagation using cotyledon explants can produce up to 100 seedlings per embryo.     

Tissue culture micropropagation of Douglas-fir

A procedure was developed for plantlet production from embryos of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. The Medium for Conifer Morphogenesis, used at full strength, and supplemented with 10 M benzyladenine for 17 days produced an average of 6.8 shoots on more than 90% of the embryos. The percentage of shoot-forming embryos as well as the average number of shoots per embryo varied significantly among eight seedlots. For secondary multiplication, 89% of the adventitious shoots produced axillary buds on MCM with 5 M benzyladenine. However, 0.5 M BA was more suitable for the elongation of axillaries. Rooting ranged from 0–87% depending upon the treatment. The highest percentage was obtained with a 7-week incubation in peat:perlite containing 1/5-strength medium, 1% sucrose and 2.7 M naphthaleneacetic acid, followed by 5 weeks on peat:perlite with 1/5-strength major and minor salts, full iron and organics, 1% sucrose and 0.1% charcoal.     

In vitro and ex vitro rooting of micropropagated shoots using three green ash (Fraxinus pennsylvanica) clones

Shoot multiplication using seedling materials was achieved by subculture on Murashige and Skoog salts with Gamborg's B5 vitamins (MSB5) medium containing a combination of 5 M 6-benzyladenine (BA), 5 M thidiazuron (TDZ), and 1 M indole-3-butyric acid (IBA) with three green ash (Fraxinus pennsylvanica) clones, SD1009 (South Dakota origin), SD2002 (South Dakota origin), and KA2018 (Kansas origin). Shoots were rooted using in vitro and ex vitro methods. For in vitro rooting studies, elongated shoots were transferred to rooting plugs supplied with liquid MSB5 medium containing a 3×3 factorial arrangement of two different auxins, -naphthaleneacetic acid (NAA) and IBA, at three concentrations (0, 5, and 10 M). The most effective treatment for in vitro root number, root length, and shoot height was 5 M IBA. The three clones also were tested for ex vitro rooting using a quick dip in 1 mM NAA, 1 mM IBA, or control (no auxin). The maximal ex vitro rooting response occurred when shoot explants of the three clones were dipped in 1 mM IBA. Significant clonal differences were noted in response to in vitro and ex vitro rooting treatments. Rooted plantlets were acclimated to the greenhouse.     

Zur Technik der Untersuchung von Erdbeerpflanzen und Chrysanthemenblättern auf Blattälchen (Aphelenchoides fragariae undA. ritzemabosi)

Zusammenfassung Die Befallsstärke von Erdbeerpflanzen durch Blattälchen wird allgemein in Älchen je 20 g Herzen ausgedrückt. Man versteht unter dem Herz einer Erdbeerpflanze die Knospe, die jungen Blütenanlagen und die unentfalteten Blätter. Es besteht aber ein gewisser Ermessensspielraum in der Zuordnung zu gefaltet bis zu voll entfaltet. Meist ist die Knospe am dichtesten befallen. Der Durchschnittsbefall sinkt deshalb um so tiefer, je mehr nicht zur Knospe gehörende Pflanzenteile mituntersucht werden. Es handelt sich um die Frischgewichte der Herzen. Diese unterliegen Schwankungen je nach Jahreszeit, Erdbeersorte und Witterung. Ferner sind die Herzen stark befallener Pflanzen leichter als die gesunder Pflanzen. Im Durchschnitt wiegen im Frühjahr 30 Herzen 20 g. Es wird vorgeschlagen, die veränderliche Bezugsgröße 20 g Herzen durch das konstante Maß 30 Pflanzen oder 30 Rosetten zu ersetzen.

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Summary On the isolation of bud and leaf nematodes (Aphelenchoides fragariae andA. ritzemabosi) from strawberry and chrysanthemum. Part II.The degree of infestation to strawberry plants by the leaf and bud nematodes is usualy expressed in nematodes per 20 g hearts. The heart of a strawberry plant includes the bud, the young inflorescences and the young closed leaves. A certain degree of judgement is requirred to distinguish between a closed leaf and an opened one. As the nematodes are usualy most concentrated in the bud, the degree of infestation is brought down by the inclusion of parts of the plant that do not directly belong to the bud.The weight of the hearts is also natably affected by weather conditions, season and plant variety. Furthermore heavily infested hearts are lighter than healthy ones. In spring, 30 hearts weigh approximately 20 g. It is suggested that the unstable measure of 20g hearts be substituted by the more reliable amount 30 plants or 30 rosettes.

     

Die Übertragung des Enationenvirus der Erbse durch verschiedene Morphen der BlattlausartenMacrosiphum euphorbiae (Thomas) undAcyrthosiphon pisum (Harris)

Zusammenfassung Unterschiedliche Vektoreignungen können zwischen Rassen, Morphen und Altersstadien einer Blattlausart auftreten. Es wurden verschiedene Morphen vonMacrosiphum euphorbiae (Thomas) undAcyrthosiphon pisum (Harris) hinsichtlich ihrer Übertragungsfähigkeit für das persistente Enationenvirus der Erbse geprüft. Dabei erwiesen sich ovipare Weibchen und adulte Männchen vonM. euphorbiae als ungeeignete Überträger, während die gleichen Morphen von zweiA. pisum-Populationen die Virose übertrugen. Auch die Fundatrix desA. pisum-BastardsTrifolium pratense rot XSarothamnus scoparius grün übertrug zu einem relativ hohen Prozentsatz. Ovipare Weibchen und Männchen der inaktiven RasseLotus uliginosus gelb konnten keine Infektion bewirken; die persistente Virusübertragungsfähigkeit ist im Falle vonAcyrthosiphon pisum (Harris) offenbar ein genetisch fixiertes Merkmal.

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Summary Different vector abilities may exist between races, morphs and development instars of a given aphid species. Several morphs ofMacrosiphum euphorbiae (Thomas) and ofAcyrthosiphon pisum (Harris) were tested in their transmission ability for the persistent pea enation mosaic virus. Oviparous females and adult males ofM. euphorbiae proved insuitable for transmission, the corresponding morphs of twoA. pisum-populations, however, were successful in transmitting. The fundatrix of theA. pisum hybridTrifolium pratense red xSarothamnus scoparius green transmitted to a relatively high percentage. Oviparous females and males of the inactiv raceLotus uliginosus yellow failed completely in transmitting; in the present case ofA. pisum the transmission character of the persistent virus evidently is genetically fixed.

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Résumé Différentes aptitudes de vecteur peuvent se trouver entre les races, les morphes et les périodes d'âge d'une espèce de pucerons. Différentes morphes deMacrosiphum euphorbiae (Thomas) et deAcyrthosiphon pisum (Harris) ont été examinées en ce qui concerne leurs aptitudes de transmettre le virus persistant du pois(pea enation mosaic). En ce cas les femelles ovipares et les mâles adultes deM. euphorbiae se montraient impropres à la transmission, pendant que les mêmes morphes de deux populations deA. pisum transmettaient la virose. C'était la fondatrice du bâtard deA. pisum Trifolium pratense rouge avecSarothamnus scoparius vert qui transmettait à une pourcentage relativement haute. Les femelles ovipares et les mâles de la race «inactive» deLotus uliginosus jaune ne pouvaient pas effectuer d'infection; en cas deAcyrthosiphon pisum (Harris), l'aptitude persistante à transmettre le virus évidemment est un caractère fixé d'une façon génétique.

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, . Macrosiphum euphorbiae (Thomas) Acyrthosiphon pisum (Harris) peaenation mosaic virus). M. euphorbiae , {A. pisum} . A. pisum-Trifolium pratense Sarothamnus scoparius . «» Lotus uliginosus ; Acrythosiphon pisum (Harris) .

     

In vitro propagation of <Emphasis Type="Italic">Oroxylum indicum</Emphasis> Vent. a medicinally important forest tree

In vitro propagation of Oroxylum indicum Vent. was carried out using cotyledonary node explants. Among the different types of cytokinins used for culture establishment, 6-benzyladenine exhibited the best response with higher concentrations (8.87µM or above) for inducing multiple shoots. Inclusion of indole-3-acetic acid (2.85µM) into 6-benzyladenine-supplemented medium triggered a high frequency of response as well as a proliferation of shoots. The best medium for proliferation was Murashige-Skoog (MS) medium with 6-benzyladenine (8.87µM) and indole-3-acetic acid (2.85µM). However, incorporation of gibberellic acid (1.44µM) was mandatory to enhance shoot elongation. Repeated subculturing of cotyledonary node and in vitro developed nodal segments in MS medium with 6-benzyladenine (4.44µM) at 4-week intervals resulted in continuous mass multiplication of shoots without any evidence of decline. Root induction was best (91.6%) when MS strength was reduced to one-quarter and combined with -naphthalene acetic acid (2.69µM) and indole-3-acetic acid (5.71µM), with a high survival rate (70–72%) of plantlets hardened in either soil rite or soil:sand:soil rite (1:1:2).     

Zur Tagesdynamik des Harzdruckes und der Harzabsonderung sowie ihre Bedeutung für den Befall der Kiefer durch den TriebwicklerRhyacionia buoliana Schiff. (Lep., Tortricidae)

Zusammenfassung Es wurden in Kiefernkulturen Untersuchungen über die Tagesdynamik des Harzdruckes und der Intensität der Harzabsonderung geführt und die Ergebnisse in Beziehung zum Befallsgrad des KiefernknospentriebwicklersRhyacionia buoliana Schiff. gesetzt.Im Verlauf eines Tages variierte der Harzdruck wenig, die Intensität der Harzabsonderung dagegen stark. Die höchste Harzfluß-Intensität fällt in den Zeitraum zwischen 13 und 16 Uhr. Die Flugzeit des Wicklers, nach 18 Uhr, liegt somit außerhalb der Zeit des stärksten Harzflusses.Weder der Index der Harzfluß-Intensität noch jener des Harzdruckes zeigten eine Beziehung zur Stärke des Wicklerbefalls. Sie sind daher als Kriterien der Widerstandsfähigkeit einer Kulturkiefer gegenRh. buoliana, zumindest unter den Verhältnissen der Süd-Ukraine, nicht verwendbar.

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Summary Studies on the diurnal rhythm of resin pressure and resin secretion in connection with the degree of pine-infestation byRhyacionia buoliana Schiff.In the course of one day pines in the age of 15 years showed only a small variation in resin pressure but a great one in secretion of resin. The maximal intensity of secretion was observed early in the afternoon. The moths ofRh. buoliana are flying after this time when the secretion of resin is low.There was no connection between resin pressure and secretion of resin on the one hand and the degree of pine-infestation byRh. buoliana on the other. The two factors are not suitable for indicating the degree of pine-infestation by this tortricid.

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Inhibition ofTrichoderma cellulase activity by a stilbene glucoside fromPicea glehnii bark

The stilbene glucoside isorhapontin (5,4-dihydroxy-3-methoxystilbene-3--D-glucoside) is the major constituent of the ethyl acetate extracts fromPicea glehnii bark. Isorhapontin inhibited the hydrolytic activity ofTrichoderma cellobiohydrolase I (CBH I) for both bacterial microcrystalline cellulose and the soluble cellooligosaccharide celloheptaitol. The inhibitory effect for celloheptaitol, however, was more drastic than that for bacterial microcrystalline cellulose. The hydrolytic activity of the CBH I core domain for celloheptaitol was also inhibited by isorhapontin to a similar extent, suggesting that the interaction between isorhapontin and the core domain of CBH I is the reason for this phenomenon. The inhibition of CBH I activity by isorhapontin showed mixed noncompetitive and uncompetitive types in a concentration of the inhibitor of less than 125M. TheK _i andK _i values were estimated to be 57.2 and 33.3M, respectively. Whereas isorhapontin strongly inhibited CBH I activity, its aglycone isorhapontigenin (3-methoxy-3,5,4-trihydroxystilbene) showed almost no inhibition. Consequently, both the stilbenic and the-glucosidic structures in isorhapontin are essential for the inhibitory effect on CBH I activity. Isorhapontin also inhibited the activity ofTrichoderma endoglucanase I for celloheptaitol, whereas almost no effect was observed for the activities of both endoglucanases II and III.An outline of this work was presented at the 49th Annual Meeting of the Japan Wood Research Society, Tokyo, April 1999     

Agrobacterium-mediated transformation of cork oak (<Emphasis Type="Italic">Quercus suber</Emphasis> L.) somatic embryos

A simple method is presented for the unreported genetic transformation of cork oak (Quercus suber L.). Pro-embryo masses (PEMs) were induced on immature zygotic embryos applied to medium supplemented with 2.3 M 2,4-dichlorophenoxyacetic acid. The established PEMs were inoculated with Agrobacterium tumefaciens LBA4404/p35S GUS INT/pCAMBIA 1301 strain. Transformants were selected on hygromycin (HYG) 94 M-supplemented medium. Viable embryos constituted 13% of those selected on HYG during 4 months. Expression of -glucuronidase at 4 months following co-cultivation confirmed transformation in 5.8% embryos selected on HYG. This method forms a basis for genetic transformation of cork oak somatic embryos.     

Neuere Beobachtungen und Untersuchungen zur Kartierung von Pflanzenschädlingen

Summary In the introduction a historical survey is given about the development of the registration service of pests. It can be seen that originally informations of the appearance of pests was mostly given by agricultural and horticultural experts in special journals of agriculture and horticulture.It is well known that a registration service was set up when a Plant Protection Organisation was foundet. Its efficiency has never ceased since its beginnings. These efforts are foundet intensionally to register the existence of pests as completely as possible.The registration service starting first of all from the damage caused by the pests maybe well understood.Our research work being a continuous contribution to the systematic registration of pests generally shows in a number of examples the heterogeneous character of the problem which must be studied concerning the individual pests and difficulties which arise. This is shown with regard to someElaterides and also because of experiments withLaspeyresia funebrana Tr. during several years.In accordance withKirchner and F. P.Müller, Rostock, we propose to register systematically a number of pests on index cards according to uniform principles in the whole of Europe, to register the vertical and horizontal appearance of pests with the same measure, in the same periods of classification and in the same way (seeAuersch 1 to 3).We are encouraged to do so by a number of appreciated views of specialists from Germany and foreign countries however it is, not enaugh to see the great importance of the card index (card-register) and to acknowledge its practicability, but it can only prove its efficiency if it is generally introduced.The systematic registration of pests will enable us to gather in the course of time empirical data, the reliability and evidence of which will steadily increase.

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Résumé On commence par livrer un aperçu historique sur le développement du système de rendre compte relativement aux plantes nuisibles. En lisant ces comptes rendus on peut reconnaître que les rapports donnés au début sur l'apparition des plantes nuisibles furent livrés en substance par la pratique agricole et jardinière par des revues spéciales de l' agriculture et de l'horticulture.Il est connu qu'avec l'établissement du service pour la protection des plantes on a établi un service de comptes rendus, au perfectionnement duquel on a travaillé dès son existance.Ces efforts furent influencés et activés par l'idée de saisir l'apparition des plantes nuisibles aussi complétement que possible. Le service des comptes rendus s'est orienté d'abord en première ligne aux dommages produits, ce qui est aisé à comprendre.Puisque notre traité doit être en général une contribution ultérieure à la connaissance systématique de l' apparition des plantes nuisibles, on explique au moyen de plusieurs exemples, comment sont différentes les questions se rapportant aux plantes nuisibles individuelles, questions qu'il faut ainsi prendre en considération et quelles difficultés en résultent. Cela est démontré pour quelques élatérides de même que sur la base d'examinations exécutées pendant plusieurs années avecLaspeyresia funebrana Tr.En concordance avecKirchner et F. P.Müller, Rostock, nous proposons de saisir systématiquement en classeurs à fiches une série de plantes nuisibles selon des points de vue uniformes, cela veut dire d'enregistrer dans les fiches proposées l'apparition verticale et horizontale des plantes nuisibles avec des mesures égales, dans les mêmes périodes d'examen et appréciation des qualités productives du sol et dans la même manière (voireAuersch 1 à 3).Nous sommes encouragés à faire ces propositions avant tout par des assentiments à notre disposition, qui nous ont été envoyés de la part des cercles compétents de notre pays et de l'é tranger. Il ne suffit pas cependant de reconnaître le fichier dans sa signification variée et d'apprécier son utilisation; le fichier peut faire ses preuves plutôt seulement, s'il est introduit en général. Au cours du temps il sera possible d'obtenir des valeurs expérimentelles par le fichement systématique des plantes nuisibles, dont la sûreté et faculté de déposition augmente constammant avec la durée de leur existence.

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õ . : . . . . õ . . , . Elateriden uLaspeyresia funebrana Tr. . . . , , , . . ( 1 3.) . . , . . .

     

Five heavy metals in propagules of ten mangrove species of China

Five heavy metals in the propagules of 10 mangrove species in China have been investigated. The results revealed that the levels of five heavy metals in most of the propagules were lower than the background levels of the soil. The levels of copper, manganese, zinc, cadmium, and lead in the propagules varied at about 2.1–7.8 g/g, 3.9–28.0 g/g, 5.7–60.0 g/g, 0.014–0.057 g/g, and 0.018–0.038 g/g, respectively. On average, the levels of five heavy metals were in the order Zn > Mn > Cu > Cd > Pb. The biological absorption coefficients were 0.02–1.30 for copper, manganese, zinc, and cadmium and about 0.0007–0.0020 for lead in propagules, showing the following order: Zn > Cd > Cu > Mn > Pb. The above results indicate that the five heavy metals have low accumulation in most of the propagules of 10 mangrove species and are at safe levels for the effective utilization of mangrove resources.     

Field measurements of gas exchange in Avicennia marina and Bruguiera gymnorrhiza

Diurnal gas exchange characteristics were measured simultaneously in two mangrove species, Avicennia marina and Bruguiera gymnorrhiza, over 7 d in summer (February–March), to compare their productivity. The study was undertaken in the Beachwood Mangroves Nature Reserve, Durban, South Africa, using fully expanded leaves of young and mature trees at the top of the canopy. Gas exchange was strongly influenced by photosynthetic photon flux density (PPFD), leaf temperature and the accompanying leaf to air vapour pressure deficit ( w). Carbon dioxide exchange was saturated at a PPFD of about 600 mol m^-2s^-1 in B. gymnorrhiza compared to 800 mol m^-2s^-1 in A. marina. Maximal CO₂ exchange occurred between 12h00 and 14h00 and was consistently greater in A. marina (8.8 mol m^-2s^-1) than in B. gymnorrhiza (5.3 mu;mol m^-2s^-1). Mean internal CO₂ concentrations ( c_i) were 260 l l^-1 in A. marina and 252 l l^-1 in B. gymnorrhiza. Photorespiratory activity was 32% in A. marina and 30% in B. gymnorrhiza. Mean water use efficiency (WUE) was 8.0 mol mmol^-1 in A. marina and 10.6 mol mmol^-1 in B. gymnorrhiza. Diurnal leaf water potentials ranged from –0.8 to –3.5 MPa and were generally lower in A. marina.     

Der Faktor der Biozönose in den Tollwutepizootien

Summary It is stated that in the spreading of epizooties among small wild animals, a factor of biocoenosis played a main role, first of all some phenomena among small rodents, the field mice. In the spreading of rabies infection, a role is ascribed to an insect, theNecrophorus vespillo.The author suggests a research work to be done in that respect, and perhaps in the regions where rabies was never registered and are still free of rabies, as to establish what in a such biocoenosis is lacking that could be a factor of rabies epizooty.

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Résumé A l'extension de grandes épizooties chez les animoux féroces, on considère que le grand rôle joue le facteur de biocenose, tout d'abord certains phénomènes entre les menus rongeurs, souris de champs. On attribute surtout le rôle de l'extension de l'épizootie de rage à l'insectNecrophorus vespillo. On recommende de faire des recherches dans ce but avec les autres branches de sciences naturelles et peut-être dans ces regions du monde où il n'y en avait pas et il n'y a pas aujourd'hui de rage, pour établir ce qu'il manque dans une telle biocenose et que peut être pourrait on prendre en considération comme le facteur des épisooties de rage.

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, — . Necrophorus vespillo L. . , .

     

Effects of nitrogen and phosphorus fertilization on the growth and nutrition of hybrid poplars on Vancouver Island

The effects of nitrogen (N) and phosphorus (P) rates, P source, and method of P application were tested on growth and nutrition of four clones of hybrid poplar (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex Marsh or P. trichocarpa×P. maximowiczii A. Henry) in a plantation on Vancouver Island. Treatments were applied shortly after planting. Nitrogen (0, 500kgNha^–1) was supplied as ammonium sulfate (AS); half of the AS was added at the start of the second growing season. Phosphorus (0, 100, 200kgPha^–1) was supplied as triple super phosphate (TSP), rock phosphate (RP), or diammonium phosphate (DAP) and banded or broadcast. Over four growing seasons, stem volumes increased with AS and P additions. The effects of AS and P each were greatest when the other was also added. Volume was greater when P was applied at 100kgha^–1 than in the P control and did not significantly increase further at 200kgPha^–1. Phosphorus additions were more effective when added as TSP or DAP than when added as RP, but only in P100 and when banded. Banding increased volume when P was applied at 200kgha^–1, but increases were significant only for DAP fertilized trees. Roto-tilling associated with the banding treatment also increased volume when AS and P were not added. Rankings of clones with respect to stem volume varied with N supply and changed over time.     

A study on some anatomical parameters of the piercing-sucking process in leaves and branches of<Emphasis Type="Italic"> Pittosporum tobira</Emphasis> L. (Pittosporaceae) infested by the cottony cushion scale,<Emphasis Type="Italic"> Icerya purchasi</Emphasis> Maskell (Homoptera: Coccina,Margarodidae)

Pittosporum tobira L. (Family Pittosporaceae) is an important plant species grown in parks and gardens in Turkey. The cottony cushion scale, Icerya purchasi Maskell (Homoptera: Coccina, Margarodidae), is an important pest of P. tobira, and causes damage mainly to the leaves, branches and stems of the host plant. In spite of the great economic importance of I. purchasi to its host plant P. tobira, information has been limited on some basic anatomical parameters of the nature of the damage to leaves and branches. The present study was, therefore, initiated to investigate the insertion and penetration of stylets into the leaves and branches of P. tobira, the length of stylets and depth of penetration in both of these tissues, and penetration of stylets into the cortex, xylem and phloem tissues and into the secretory and non-secretory canals of the leaves and branches. The results showed great variations between the leaves and branches in all the criteria observed in I. purchasi infesting P. tobira. The study found 20 whole and 23 broken mouthparts in the leaves as compared to 3 whole mouthparts and 1 broken in the branches. Length of the stylets inserted into the leaves ranged from 33.83 m to 540.93 m and into the branches from 202.85 m to 340.8 m. The stylets reached greater depths in the leaves, at 540.93 m, as compared to 498.67 m in the branches. The stylets in the leaves were associated with 12 secretory canals, but with none in the branches. It was found that three stylets reached xylem in the leaves as compared to one in the branches. Similarly, 17 stylets in the leaves reached to the phloem, but only 2 in the branches. No stylets were found in the cortex of the leaves, but one was found in the branches. In the leaves, 11 stylets reached and remained in the non-secretory canals, but none in the case of the branches.     

Samenschädlinge der Laubhölzer in der zentralen Ukraine und einige Faktoren,welche ihre Widerstansfähigkeit gegen Schädinsekten bestimmen

Zusammenfassung Samenuntersuchungen an 12 Eichenarten und -formen, 20 Birkenarten und 14 Ahornarten zeigten, daß die Samen der Eichen und Ahorne, nordamerikanischer Birken und Ahorne nordasiatischer Abstammung in der Ukraine praktisch von Schädinsekten nicht beschädigt werden, während der Samenertrag der heimischen durch Schadinsekten stark herabgemindert wird.Die Widerstandsstärke der Samen gegen Schädlinge ändert sich. nicht nur bei den verschiedenen Arten, sondern auch innerhalb der Bäume einer Art je nach ihrem physiologischen Zustand. Die Widerstandsfaktoren der Samen gegen Schädinsekten sind entweder anatomisch-morphologische Besonderheiten (Schalenstärke), oder das Vorhandensein von Schutzstoffen in den Samen oder beide zusammen. Besonders deutlich sind diese Faktoren in den Eicheln von Bäumen der nordamerikanischen Abstammung gegen Eichelrüssler ausgeprägt. Chromatographische Analyse der Auszüge aus den widerstandsfähigen Samen der Schmidt'schen Birke und des Eschenahorns zeigten, daß für die Rolle der Schutzstoffe die Phenolstoffe verantwortlich sind von denen z. B. die Chlorogensäure ermittelt wurde.

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Lignin in woody tissues after chemical pretreatment and bacterial attack

Summary Tangential latewood sections (60 m) of Scots pine sapwood were differently treated with chlorite. The subsequently incubated two strains of Bacillus polymyxa caused a weight loss up to about 25%, including a loss of lignin of about 42%.UV-microspectrophotometry of 1 m-cross-sections prepared from the cultured woody tissues demonstrated that bacteria caused neither quantitative nor qualitative changes of the remaining lignin.The lignin, which was dissociated from the pretreated woody cell wall by bacteria, could not be respired, suggesting that the lignin is a ballast to these bacteria that inhibits the dissimilation of the carbohydrates in the wood.We appreciate the financial support by Deutsche Forschungsgemeinschaft. Thanks are due to Mrs. R. Endeward for her assistance during the microspectrophotometric evaluations and to Mr. U. Engel and Dr. O. Faix for carrying out the IR-measurements     

Moisture content-water potential relationship of red pine sapwood above the fiber saturation point and determination of the effective pore size distribution

Summary The pressure membrane and pressure plate techniques were used to establish the moisture content-water potential (M-) relationship of red pine (Pinus resinosa Ait.) sapwood in desorption above the fiber saturation point. The moisture content-water potential relationship is required for the development of a model of drying considering the gradient of water potential as the driving force of moisture in wood. This relationship was established at 18, 56 and 85 °C for radial desorption. The results obtained demonstrate that water potential increases with temperature T at a given moisture content M. There is no significant variation of /T with temperature. Also, there is no plateau at intermediate moisture contents as was the case for the M- relationship of aspen sapwood established in a previous work. The effective integral and differential pore size distributions inferred from the M- relationship are also presented. The largest proportion of effective pore openings was found for a radius of 0.2 m. This value can be related to the pit membrane openings of red pine.This research project is currently supported by the Natural Sciences and Engineering Research Council of Canada under grant no. OGP0121954     

Sensory evaluation of carpet cleaner containing essential oil and the effect on mites

We prepared carpet cleaners containing three wood oils extracted from Thujopsis dolabrata Sieb. et Zucc. var. hondai Makino, Chamaecyparis obtusa Endl., and Chamaecyparis taiwanensis Masamune et Suzuki and studied their effects on mites and perfumerists' impressions. The oil concentrations were set at 0.1%, 0.2%, 0.4%, 0.8%, and 1.6%. The effects on Dermatophagoides pteronyssinus Trouessart were investigated. The sensory evaluations were conducted by seven male perfumerists using the SD method and they were asked to describe freely their impressions of the scents. These results showed that: (1) all three types of wood oil had a significant effect on making D. pteronyssinus inactive at 0.1% concentration; and (2) the wood oil of T. dolabrata evoked refreshing, natural, rich, and intellectual feelings; C. obtusa oil evoked vivacious, rich, and intellectual feelings; and C. taiwanensis evoked refreshing, natural, rich, rough, masculine feelings. According to the test subjects, T. dolabrata evoked woody and earthy impressions; C. obtusa evoked woody, citrus, and pine-resin impressions; and C. taiwanensis evoked woody, citrus, and medical impressions. Many issues remain to be investigated, but the inclusion of wood oil in carpet cleaners offered both good mite control and a pleasant aroma to humans. This study suggests a new potential for using these wood oils.