Effects of urea formaldehyde foam soil amendment on growth and response to transient water deficit stress of potted Flindersia schottiana saplings

Hydrocell™is a urea formaldehyde resin foam (UFRF) product used as a soil amendment. It is proposed to improve the physicochemical properties (viz. water relations and aeration) of the plant root zone. Flindersia schottiana is a tree species used in the ornamental horticulture industry. This study investigated the potential of Hydrocell™ [0–50% (v/v) incorporation rates] to promote growth of F. schottiana saplings during containerized nursery production in composted pine bark medium. The growth response of the potted saplings to transient water stress was also assessed. In addition, growth of F. schottiana saplings potted into three different soil types (sand, loam, clay) was assessed in the presence and absence of 30% (v/v) Hydrocell™. Under well-watered conditions in composted pine bark, Hydrocell™ treatments enhanced sapling leaflet numbers, with 30% (v/v) being the most beneficial treatment. However, no consistent significant differential effects of incorporation rate on either plant height or stem diameter were recorded. Extended time to wilting upon withholding irrigation was achieved, with the optimum Hydrocell™ rate being 10% (v/v). Among the three soil types compared, use of 30% (v/v) Hydrocell™ resulted in improved growth of potted F. schottiana saplings in the sand and loam soils, but not in the clay soil. These findings suggest that the UFRF, Hydrocell™, holds limited promise as a soil amendment for trees in the nursery phase. Optimum incorporation rates (v/v) would need to be determined for individual media types, specific irrigation regimes and individual taxa.     

Bell pepper yield and soil properties during conversion from conventional to organic production in Indian Himalayas

A conversion period of at least two years is required for annual crops before produce may be certified as organically grown. There is a need for better understanding of the various management options for implementing from conventional to organic production. The purpose of this study was to evaluate the effects of three organic amendments on growth and yield of bell pepper (Capsicum annuum L.), the benefit:cost ratio, soil fertility and enzymatic activities during conversion to organic production. For that purpose six treatments were established: composted farmyard manure (FYMC, T₁); vermicompost (VC, T₂); poultry manure (PM, T₃) along with biofertilizers (BF) [Azotobacter + phosphorus solubilizing bacteria (Pseudomonas striata)] and mix of three amendments (FYMC + PM + VC + BF, T₄); integrated crop management (FYMC + NPK, T₅) and unamended control (T₆). The bell pepper yield under organic management was markedly lower (33–53% and 18–40% less in first and second year of conversion, respectively) than with the integrated crop management (FYMC 10 Mg ha⁻¹ + NPK – 100:22:41.5 kg ha⁻¹) treatment (T₅). Combined application of three organic amendments (FYMC 10 Mg ha⁻¹ + PM and VC each 1.5 Mg ha⁻¹ + BF, T₄) and T₁ produced similar but significantly higher bell pepper yield (27.9 and 26.1 Mg ha⁻¹, respectively) compared with other organic amendment treatments. Both T₄ and T₁ greatly lowered soil bulk density (1.15–1.17 Mg m⁻³), and enhanced soil pH (7.1) and oxidizable organic carbon (1.2–1.3%) compared with T₅ and unamended control (T₆) after a two-year transition period. However, the N, P and K levels were highest in the plots under integrated management. T₁ plots showed higher dehydrogenase activity values. However, acid phosphatase and β-glucosidase activities were higher in T₆ plots whereas urease activity was greater in T₅ plots compared with other treatments. Among the treatments involving organic amendments alone, T₁ gave a higher gross margin (US $ 8237.5 ha⁻¹) than other treatments. We conclude that T₁ was found more suitable for enhancing bell pepper growth and yield, through improved soil properties, during conversion to organic production.     

Root development of Thuja plicata in peat-substitute rooting media

In this study we investigated the potential usefulness of different substrates produced from gorse (Ulex europaeus L.) for propagating cuttings of Thuja plicata ‘Atrovirens’. The gorse was composted by different methods, to produce four types of compost. These composts were then used as such (100% compost) or in mixes with coarse sand and pine bark compost (25% compost, 25% coarse sand and 50% pine bark compost, v/v/v) to prepare eight different substrates. The substrates were compared in cutting propagation trials carried out in a greenhouse, with a standard rooting medium consisting of 25% Sphagnum peat moss, 25% coarse sand and 50% pine bark compost (v/v/v). Cuttings were taken from a garden hedge in March 2003, and comprised shoots of the previous year's growth. The bases of the cuttings were treated with a 0.4% solution of IBA rooting hormone, then inserted into cells filled with the corresponding substrate and placed on a propagation bench. Rooting percentage and visual rooting score were determined in July. The quality of roots was evaluated, as total root length and number of root tips, in July, September and November 2003, by use of a Delta-T Scan system. The rooting success of the cuttings ranged from 75% to 90%, except when the substrate comprised the pure composted gorse, obtained after the shorter period in an enclosed composting system with addition of fertilizer and reuse of leachates. Most of the cuttings in this substrate died and only 4% of the cuttings rooted successfully. Physicochemical characteristics of this rooting medium included very high electrical conductivity, low C/N ratio and high water retention. Addition of coarse sand and pine bark compost to the composted gorse significantly increased the rooting success. The root quality variables differed depending on the substrate considered and date of harvest. The results showed that substrates made from gorse compost are of potential use as a peat substitute for propagating cuttings of T. plicata ‘Atrovirens’. The physicochemical properties of the composted gorse should be determined before its use to establish whether it can be used pure as a substrate for rooting or whether it should be mixed with other substances.     

Growth of Viburnum tinus in peat-based and peat-substitute growing media

To assess the growth of ornamental shrubs in peat alternative substrates, one ornamental species, Viburnum tinus L., was cultivated in a number of different substrates in two climates: a French oceanic (Oce) and a Spanish Mediterranean (Med). In Oce, three mixtures (1/1, v/v) of Finnish peat/yard compost, yard compost/raw coir and Finnish peat/raw coir were used while the expanded perlite/composted manure (1/1), forest compost/composted bark (1/1) and forest compost/cattle manure compost (2/3) were tested in Med. A mixture (1/1) of Finnish peat/pine bark compost was used in both climates as a control. Plants were cultivated at a density of six plants/m² in 4 l containers with drip irrigation. Plant height, dry mass, and leaf area were measured at intervals during cultivation. In Oce, substrates with yard compost or raw coir produced plants of similar size to those in the control substrate. In Med, forest compost/cattle manure compost mixture produced plants with the same height as those in the control mixture and the two other mixtures produced shorter plants. In both climates, substrates ranked the same whether height, dry mass or leaf area were considered. Using those parameters, significant effects of different substrates were revealed. Those effects are related to the substrate characteristics, mainly physical ones. As peat is used in a large range of situations, the experiment showed that substrate performance varies with its use, so alternative substrates can show better performance than those using peat.     

Effects of explant type, culture media and growth regulators on callus induction and plant regeneration of Chinese jiaotou (Allium chinense)

To establish an efficient protocol of shoot regeneration from callus, effects of explant type, culture media and plant growth regulators on callus induction and shoot regeneration of Chinese jiaotou (Allium chinense) were evaluated. The results showed that basal plate was the best explant for callus induction (47.5%) when cultured on B5 medium supplemented with 0.1 mg l⁻¹ 6-benzylaminopurine (BA) and 1.0 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D), and B5 was the best medium to induce callus formation with 49.3% of the explants forming callus. The highest callus induction (65.2%) was achieved culturing basal plate on B5 medium supplemented with 0.1 mg l⁻¹ BA and 1.0 mg l⁻¹ 2,4-D after 8 weeks of culture. The best callus proliferation was observed on B5 medium with 1.5 mg l⁻¹ 2,4-D. Shoots regenerated at the highest frequency of 58.8% with 4.5 shoots when calli were cultured on B5 medium with 0.1 mg l⁻¹ BA and 1.0 mg l⁻¹ a-naphthaleneacetic acid (NAA). This protocol provides a basis for future studies on genetic improvement and could be applied to large-scale multiplication systems for commercial nurseries of Allium chinense.     

Relative salt tolerance of selected herbaceous perennials and groundcovers

In order to use reclaimed water to irrigate landscape plants and minimize damage and loss, salinity tolerance of commonly used landscape plants needs to be determined and characterized. Eight herbaceous perennials and groundcovers were obtained from a local nursery, transplanted to 2.6-L plastic containers and grown in the greenhouse for 2 weeks before saline irrigation at electrical conductivity (EC) of 0.8 (tap water), 3.2, 6.4, or 12 dS m⁻¹ were initiated. Plants were irrigated with measured amount of saline solutions to obtain 30% leaching when approximately 50% water had been depleted. After 12 weeks, four plants in each treatment were destructively harvested and dry weights of shoots and roots were determined. Three Penstemon species (P. eatonii A. Gray, P. pseudospectabilis M.E. Jones, and P. strictus Benth.) and Lavandula angustifolia Mill. at 6.4 and 12 dS m⁻¹ and most at 3.2 dS m⁻¹ did not survive. Shoot dry weight of Delosperma cooperi (Hook.f.) L. Bolus decreased by 25% at 12 dS m⁻¹, but there were no significant differences among the rest of the treatments. All plants of Teucrium chamaedrys L. survived but growth was reduced significantly with lower visual scores as salinity of irrigation water increased. Although growth was reduced in Gazania rigens (L.) Gaertn. as salinity increased, no other signs of stress or injury were observed. Ceratostigma plumbaginoides Bunge had reduced growth at 3.2 dS m⁻¹ and higher EC levels compared to the control, older leaves showed reddish pigmentation at 6.4 dS m⁻¹, whereas those at 12 dS m⁻¹ did not survive. Ion concentrations of shoot and root tissue at the end of the experiment on surviving plants were also affected by salinity levels and varied among species. Among the tested species, D. cooperi and G. rigen indicated a relatively high tolerance to salinity, T. chamaedrys and C. plumbaginoides were moderately tolerant, and the rest were less tolerant.     

Varietal differences in phenolic content and astringency in skin and flesh of hardy kiwifruit resources in Japan

Varietal differences in the total phenolic content and astringency in the skin and flesh were determined among the cultivars and local collections of hardy kiwifruit with a ploidy variance found in Japan. The average values of the total phenolic content in the skin and flesh were 2.66 and 0.18 g 100 g⁻¹ FW, respectively. There were large varietal differences in the total phenolic content in the skin in the range of 1.3–5.0 g 100 g⁻¹ FW. Kochi (tetraploid), while Gassan and ‘Mitsuko’ (hexaploid) contained a larger amount of total phenolics. High astringency was found in Gassan, ‘Mitsuko’ and ‘Hoko’ (hexaploids) and Kochi. HPLC analysis showed that the major components of phenolics in the flesh were (+)-catechin, chlorogenic acid, rutin, (−)-epicatechin and quercetin.     

Physico-chemical and antioxidant properties of six apple cultivars (Malus domestica Borkh) grown in southern Brazil

The objective of this study was to compare the physico-chemical properties and antioxidant activity of six apple cultivars grown in southern Brazil. Apple peel color, dry matter, total soluble solids, pH, total sugars, titratable acidity, total phenolics, total monomeric anthocyanin and total antioxidant activity were measured in the apple cultivars Imperatriz, Daiane, Fred Hough, Fuji Suprema, Galaxy and Baronesa. The results showed great quantitative differences in the composition of the apple cultivars. Of all the cultivars, the peel of Galaxy was slightly more red-colored and that of Fred Hough was the least red-colored. The dry matter varied from 15.24% (Galaxy) to 19.55% (Fuji Suprema), the soluble solids content was between 11.8 (Fred Hough) and 14.0 (Daiane) °Brix, pH values varied from 3.90 (Imperatriz) to 4.27 (Fred Hough), the total sugar content (g 100 g⁻¹) ranged from 11.54 (Imperatriz) to 14.78 (Fuji Suprema) and the titratable acidity content (g 100 g⁻¹) varied from 0.20 (Baronesa) to 0.36 (Imperatriz). The total phenolic content (GAE 100 g⁻¹ fresh matter) observed in the apple cultivars was between 105.4 (Baronesa) and 269.7 mg (Imperatriz). The values of the total anthocyanin content (mg 100 g⁻¹ FM) ranged from 4.79 (Fred Hough) to 41.96 (Galaxy). The highest total antioxidant activity was observed in Imperatriz (739 μmol TEAC 100 g⁻¹ FM), while the lowest value was found in Fuji Suprema (335 μmol TEAC 100 g⁻¹ FM). There was a strong correlation between total monomeric anthocyanin content and all peel color measurements and between antioxidant activity and total phenolic content. The results suggested that genotype is the main factor that determines the composition of bioactive compounds in apples and this provides important information on how to make the best use of the apple cultivars investigated.     

Effect of the culture environment on stomatal features, epidermal cells and water loss of micropropagated Annona glabra L. plants

Shoots of Annona glabra L. were rooted in vitro under three levels of irradiance and two closure systems (conventional and natural ventilation) of the culture vessels. Once the shoots had been rooted, we studied how the manipulation of the culture environment affects the stomata features and water loss through leaf tissues after the plants are removed from the vessel. The stomata frequency increased significantly in the leaves of plants grown under high (300 μmol m⁻² s⁻¹) compared to low (50 μmol m⁻² s⁻¹) or intermediate (150 μmol m⁻² s⁻¹) irradiance, with higher effect under natural ventilation. Irrespective of the culture environment, leaves developed in vitro attained a higher stomata frequency than those grown in vivo. Under high irradiance and natural ventilation, the leaves presented functional stomata of characteristically elliptical shape and the epidermal cells were smaller and had slightly sinuous anticlinal walls. Besides, water loss through leaves of plants grown under high irradiance and natural ventilation was drastically reduced if these plants were exposed to an environment with low relative humidity thereafter. Our results indicate that an increased light availability and the use of natural ventilation improve the regulatory capacity of water loss in micropropagated A. glabra L. plants and can favor the plants’ survival and growth after transference to the natural environment.     

Effects of nutrient solution electrical conductivity and sulfur, magnesium, and phosphorus concentration on sesquiterpene lactones in hydroponically grown lettuce (Lactuca sativa L.)

The effect of nutrient solution electrical conductivity (EC) and sulfur (S), magnesium (Mg), and phosphorus (P) levels on the content of the primary sesquiterpene lactones (SLs), lactucin, 8-deoxylactucin, and lactucopicrin, in hydroponically grown lettuce was assessed. Lettuce grown at 4 EC levels (0.5, 1.0, 2.0, and 3.0 dS m⁻¹) displayed significant differences in leaf area index, number of leaves, plant height, fresh weight per plant, and chlorophyll content that were highest at EC 2.0 dS m⁻¹. Lactucin (5.5 μg g⁻¹ dry weight), 8-deoxylactucin (7.5), lactucopicrin (35.8), and total SLs (48.7) concentraions were highest at EC 0.5 dS m⁻¹. Four S (8, 16, 48, and 80 mg L⁻¹) and Mg (6, 12, 36, and 60 mg L⁻¹) levels and 3 P (8, 16, and 48 mg L⁻¹) levels were assessed for their effect on individual and total SLs. S and P had the greatest effect on SL levels. Plants in the lowest S level had significantly higher lactucin, lactucopicrin and total SLs. Each of the SLs was higher in the highest P level while Mg influenced only the lactucopicrin level in a quadratic manner. The results indicate that solution culture conditions can strongly influence the SL concentration and therefore bitterness and acceptability of lettuce.     

Germination and growth of sponge gourd (Luffa cylindrica) pollen tubes and FTIR analysis of the pollen tube wall

Emergence of multiple pollen tubes from single pollen grains occurred both in vitro and in vivo in sponge gourd (Luffa cylindrica (L.) Roem). The frequency with which pollen grains produced multiple pollen tubes in vivo (7.2%) was lower than that under in vitro conditions (14.9%). In pollen grains germinated in vitro, the total length of the multiple pollen tubes was greater than that of single pollen tubes, but individual tubes among the multiple tubes did not reach the same length as single tubes. Moreover, the growth of the single pollen tubes continued for a longer period in vitro than that of the multiple tubes. Fluorescence microscopy showed that callose was present throughout the pollen tube wall except in the apical part of growing pollen tubes, and nuclei moved into the longest of the multiple tubes. Results of Fourier transform infrared microspectroscopy indicated that abnormal cell wall components (peaks at 800–1000 cm⁻¹) were more frequent in multiple pollen tubes lacking nuclei, and the pectin content (1733 cm⁻¹) in multiple pollen tubes was much lower than that in single pollen tubes. These findings suggested that there were significant differences in pollen tube growth and wall composition between single and multiple pollen tubes, and that multiple pollen tubes had much less opportunity than single pollen tubes to reach the embryo sac and achieve double fertilization.     

Establishment of in vitro tissue cultures from Echinacea angustifolia D.C. adult plants for the production of phytochemical compounds

The establishment of in vitro cultures of Echinacea angustifolia D.C. was obtained directly from sections of flower stalks of adult plants. The shoot formation was obtained from this plant material placed on a modified MS basal medium named CH supplemented with 0.5 mg L⁻¹ 6-benzylaminopurine (BA). The in vitro propagation procedure of E. angustifolia consisted of three distinct phases: an initial regeneration phase from stalk sections (IP shoots on basal medium with 0.25 mg L⁻¹ BA), an elongation phase on active charcoal and an axillary proliferation of the shoots (AP shoots on basal medium with 0.5 mg L⁻¹ BA).Regenerating calli were established from leaves of in vitro shoots cultured on CH medium supplemented with 3 mg L⁻¹ BA and 0.5 mg L⁻¹ indole-3-butyric acid (IBA). Developed shoots from the callus cultures were subcultured on the CH medium with 0.5 mg L⁻¹ BA (leaf regenerated shoots: LR shoots). The secondary metabolite content of the in vitro plant material was compared with that of the greenhouse growing plants. The quali-quantitative LC-DAD-ESI-MS analysis on the extracts from axillary proliferation shoots (AP shoots) showed significant production of caffeic acid derivatives while leaf callus and LR shoots, accumulated mainly alkamides. These results showed that the proper choice of the procedures for in vitro multiplication allowed us to obtain plant biomass able to produce the active compounds typical of E. angustifolia plants.     

In vitro and in vivo assessment of the potential of compost and its humic acid fraction to protect ornamental plants from soil-borne pathogenic fungi

Five humic acid fractions (HAs) were isolated from various organic substrates used for plant growth in containers, including both conventional ones, i.e. peat (P) and coconut fiber (CF), and newly introduced ones, i.e. compost (Co) and mixtures at 20% and 60% (v/v) of Co with P (Co20 + P and Co60 + P) and Co with CF (Co20 + CF and Co60 + CF). The effects of these HAs at 50 and 300 mg L⁻¹ were evaluated on the mycelial growth of two soil-borne phytopathogens, Pythium ultimum (PYT) and Fusarium oxysporum f. sp. callistephi (FOC), in a series of in vitro experiments. Furthermore, in experiments performed in a climatic chamber, the bulk substrates were tested on the growth and health of two ornamental plant species: impatiens (Impatiens walleriana Hook) and China aster (Callistephus chinensis L. Nees), repeatedly infected respectively with PYT and FOC. In general, any HA at either dose reduced significantly the radial growth of PYT mycelium after 42 h, whereas Co-HA and P-HA at the higher concentration showed a high inhibition on PYT during the whole experiment. A significant inhibition of FOC growth was exerted by all HAs, especially at the higher dose, during the 192-h experimental time. No significant correlation exists between the extent of the inhibitory action on the two fungi and the chemical and functional properties of HA. The only exception is the existence of a negative correlation between the inhibition of PYT and the HA oxygen content. The various substrates used in trials conducted in vivo caused different plant growth response. The treatments with Co alone or its mixtures at 60% with P or CF were lethal for both plants, even before the first fungal inoculation. The substrates Co20 + P and Co20 + CF were very effective in controlling the fungal attack and increasing the growth of impatiens and China aster.     

The inhibition of flowering by means of gibberellic acid application reduces the cost of hand thinning in Japanese plums (Prunus salicina Lindl.)

The application of gibberellic acid during flower bud induction significantly reduced flowering of ‘Black Diamond’ and ‘Black Gold’ Japanese plums. The response depended on the concentration applied and on the type of shoot. Mixed shoots had a similar response in both varieties, flowering being reduced by 40% for GA₃ 50 mg l⁻¹ and by 75–90% for GA₃ 75 mg l⁻¹ or higher concentration. With regard to spurs, GA₃ 50 mg l⁻¹ reduced flowering intensity by 40% and 25% in ‘Black Gold’ and ‘Black Diamond’, respectively, and GA₃ 75 mg l⁻¹ or higher concentration reduced flowering by 70% and 50%, respectively. This partial inhibition of flowering significantly reduced the cost of manual thinning. The best GA₃ concentration was found to be 50 mg l⁻¹, since it reduced the cost of thinning by 45–47% and increased final fruit weight by 7–33% for ‘Black Diamond’ and ‘Black Gold’, respectively. Not significant differences in yield and in mature fruit characteristics of treated trees were found compared to untreated trees.     

Sod establishment and turfgrass growth as affected by urea–formaldehyde resin foam soil amendment

Urea–formaldehyde resin foam (UFRF) has recently been introduced as a soil amendment for turfgrass culture. A field study was performed to evaluate the impact of UFRF on soil physical and chemical properties, sod establishment and turfgrass shoot and root growth. Treatments included a non-amended, sandy loam soil and the same soil amended with UFRF at a rate of 20% (v/v) incorporated into the upper 0.1 m of the substrate. Physical and chemical analyses of the two substrates involved the determination of bulk density, total porosity, air-filled porosity at 0.4 m, moisture release curve, pH and electrical conductivity. Turfgrass growth was determined through several measurements that included shoot growth rate, root growth rate and monitoring of three root architecture parameters namely, total root length, total root area and mean root diameter. Turfgrass establishment was evaluated by measuring the vertical force applied to detach the sod from the substrate.UFRF amendment did not influence pH and EC and provided minimal alterations (non-significant) in soil physical properties by slightly increasing total porosity, easily available water and air-filled porosity, and by reducing bulk density. The impact of UFRF amendment improved cumulative clipping yield in five sampling dates but root growth and sod establishment rate were better than the control only on one sampling date. There appears to be limited benefit in amending soil with UFRF.     

Flavonol and phenolic acid composition of sweet cherries (cv. Lapins) produced on six different vegetative rootstocks

The effect of rootstock (‘MaxMa 14’, ‘Weiroot 13’, ‘PiKu 1’, ‘Weiroot 158’, ‘Gisela 5’ and ‘F12/1’) on phenolic acid and flavonol content of “Lapins” sweet cherry was investigated. Phenolic acids and flavonols were isolated from sweet cherries and analyzed by using reversed phase high-performance liquid chromatography (RP-HPLC). The major phenolic acids in sweet cherries were neochlorogenic acid (18–50 mg kg⁻¹), chlorogenic acid (19–62 mg kg⁻¹) and p-coumaric acid derivatives (15–125 mg kg⁻¹). The amount of flavonol quercetin-3-rutinoside (8–37 mg kg⁻¹) was significant as well. There are significant variations in the phenolic compound content among sweet cherry fruits grown on trees grafted on different vegetative rootstocks. The significantly higher chlorogenic acid, neochlorogenic acid, p-coumaric derivative and quercetin-3-rutinoside contents were found in sweet cherry fruits grown on trees grafted on ‘Weiroot 13’ and ‘PiKu 1’ rootstocks. Sweet cherries produced on trees grafted on other rootstocks had significantly lower phenolic compound content.     

Energy balance of six common landscape surfaces and the influence of surface properties on gas exchange of four containerized tree species

Gas exchange and growth of woody landscape plants is strongly affected by underlying surfaces. In urban areas, plants are subjected to energy balance characteristics of a variety of surfaces. We investigated energy balance properties of six urban surfaces: asphalt, gravel rock mulch, lava rock mulch, concrete, pine bark mulch, and turf. Each summer over a 3-year period, incoming global shortwave radiation, surface temperature, surface reflectivity (albedo), soil temperature below each surface, and soil heat flux were measured for each surface, and total incoming radiation, thermal conductivity, and longwave radiation emitted by each surface were calculated. Differences in surface properties were analyzed by regression analysis. Albedo was greatest for concrete and least for lava rock mulch, while thermal conductivity was greatest for asphalt and least for lava rock and pine bark mulches. Under maximum incoming total radiation, regression analysis indicated: soil heat flux was greatest under asphalt and concrete and least under lava rock and pine bark mulches; soil temperature below each surface was greatest for asphalt and concrete and least for pine bark mulch; surface temperature was greatest for pine bark mulch and least for turf; and longwave radiation flux of each surface was greatest for pine bark mulch and least for turf. This research revealed that more energy was conducted into the soil below asphalt and concrete, and that a greater portion of incoming radiation was prevented from entering the soil below pine bark and lava rock mulches than below other surfaces. Due to these effects, and the lack of evaporative cooling, surface temperatures were greater, and more longwave radiation was emitted from non-vegetative surfaces than from turf. In a concurrent study, we investigated if the energy balance of turf, pine bark mulch, and asphalt surfaces influenced gas exchange of four containerized tree species grown over each surface. On several occasions over a 2-year period, morning-to-evening measurements of stomatal conductance, leaf temperature, and plant water loss were made on containerized Bechtel crabapple (Malus ionensis ‘Plena’), Norway maple (Acer platanoides ‘Crimson King’), globe willow (Salix matsudana ‘Navajo’) and American plane tree (Platanus occidentalis). Leaves over pine bark mulch and asphalt intercepted more longwave radiation and generally had greater leaf temperature and leaf-to-air vapor difference than leaves over turf. As a result, trees over non-vegetative surfaces generally had lower stomatal conductance and water loss than trees over turf.     

Effects of an in vitro maturation treatment on plant recovery from avocado zygotic embryos

An efficient protocol for in vitro maturation of very immature, <10 mm, avocado embryos has been developed. The efficiency of plant recovery as well as the quality of the resulting plants was greatly improved by including a maturation phase prior to induction of germination. The influence of different factors, such as the gelling agent, organic supplements or abscisic acid, on embryo maturation and subsequent germination was tested. Optimum conditions were met when maturation was carried out in B5m medium supplemented with the Jensen's amino acids, an extra 88 mM sucrose and 6 g l⁻¹ agar as gelling agent. At these conditions, embryos which had been collected 68 days after pollination germinated at a 65% rate in solid medium, giving rise to healthy and vigorous plantlets. Anatomical differentiation and storage product accumulation occurring during the in vitro maturation phase were studied by means of histological techniques. Results obtained revealed that, at the end of the in vitro maturation period, embryos resembled the pattern previously established for avocado embryos matured under in vivo conditions: histodifferentiation had been accomplished and starch granules and protein bodies were abundant.     

Use of a cyclic high-pressure sodium lamp to inhibit flowering of chrysanthemum and velvet sage

Photoperiod is commonly controlled in the commercial production of ornamental crops to induce or prevent flowering. Flower induction in short-day (SD) plants can be prevented or delayed when the natural daylength is short by providing low-intensity lighting during the dark period. A stationary high-pressure sodium (HPS) lamp with an oscillating aluminum parabolic reflector (cyclic HPS) has been developed to provide intermittent lighting to greenhouse crops. We determined the efficacy of a cyclic HPS lamp at preventing flowering in SD plants garden chrysanthemum [Chrysanthemum × grandiflorum (Ramat.) Kitam.] ‘Bianca’, pot chrysanthemum ‘Auburn’, and velvet sage (Salvia leucantha L.) relative to traditional night interruption (NI) lighting strategies. Plants were grown in a glass-glazed greenhouse at a mean daily temperature of 19.5–20.7 °C with natural SD photoperiods. NI lighting was delivered during the middle of the night (2230–0230 h) from a 600 W cyclic HPS lamp mounted at one gable end of the greenhouse or from incandescent (INC) lamps that were illuminated for the entire 4 h (CONT INC) or for 6 min every 30 min for 4 h. Plants under cyclic HPS were grown at lateral distances of 1, 4, 7, 10, or 13 m from under the lamp. Control plants were grown under an uninterrupted 15 h skotoperiod. As the distance from the cyclic HPS lamp increased from 1 to 13 m, the maximum irradiance measured during the NI decreased from 25.4 to 0.3 μmol m⁻² s⁻¹ and time to visible inflorescence (VI) and the number of nodes at VI decreased. All species had a VI within 54 d, but ≤10% of plants flowered when grown at a lateral distance of 1 or 4 m from the cyclic HPS lamp or under CONT INC. Plants grown without NI had a VI 2 to 15 d earlier and flowered 7 to 24 d earlier than plants grown at 10 or 13 m from the cyclic HPS. All garden chrysanthemums flowered under cyclic INC, whereas velvet sage and pot chrysanthemum had 15% and 35% flowering, respectively. These results indicate that a cyclic HPS lamp can be used effectively to delay flower induction and prevent flowering in these species when NI is delivered at ≥2.4 μmol m⁻² s⁻¹.     

Effects of flurprimidol and daminozide on growth and flowering of oriental lily ‘Mona Lisa’

Two separate experiments were conducted to evaluate the effectiveness of growth retardants in controlling height of oriental lily ‘Mona Lisa’. In first experiment flurprimidol was applied as a single foliar spray (0, 10, 20, 30 and 40 mg l⁻¹) or a soil drench (0, 0.1125, 0.225, 0.3375 and 0.45 mg/pot) at two plant growth stages (7 and 15 cm). In second experiment flurprimidol (0, 10, 20, 30 and 40 mg l⁻¹) and daminozide (0, 2500, 3500 and 4500 mg l⁻¹) were applied once or twice as a foliar spray. Daminozide applied once or twice at concentrations of 2500–4500 mg l⁻¹ was ineffective in ‘Mona Lisa’ growth retardation. Flurprimidol was effective growth retardant in reducing stem extension without adverse side-effects. The maximum reduction in plant height obtained was 45% compared to controls. Flowering time was not affected by lower rates of flurprimidol, but it was slightly delayed when retardant was applied at higher doses. The tepal size, leaf size and pedicel length were smaller than those of the control plants. Intensified leaf colour was observed on flurprimidol treated ‘Mona Lisa’. The effect of flurprimidol persisted in the year after treatment.