Micropropagation of western hemlock (Tsuga heterophylla [Raf.] Sarg.) from embryonic explants

An in vitro propagation protocol is described for western hemlock, an important forestry species in Canada. For shoot bud induction, embryonic explants were placed initially on one-third strength Schenk and Hildebrandt medium containing 5 M N⁶-benzyladenine (BA) or 5 M BA in combination with either 5 M kinetin or 5 M 2-isopentenyl adenine for 14 days. The explants were transferred to basal medium without cytokinins for 3 weeks, and then to basal medium containing 0.05% activated charcoal. Elongating shoots were subcultured every 4 weeks on charcoal medium. Shoots, 10 mm in stem height, were rooted either in agar or sterilized peat/perlite (1:1). Up to 70% of the shoots formed roots when they were transferred to the latter, moistened with 1/2 strength Gresshoff and Doy medium containing 5 M -naphthaleneacetic acid. About 90% of the plantlets survived transfer to greenhouse conditions.     

Cultural factors influencing adventitious shoot and plantlet formation from slash pine cotyledons

Cultural factors affecting in vitro shoot and subsequent plantlet formation of slash pine (Pinus elliotti Engelm.) cotyledons were investigated. Basal media composition, N⁶-benzylaminopurine (BAP) concentration and exposure time significantly influenced bud induction in cotyledons cultured under a continuous photoperiod of 35–40 mol m^–2 s^–1 at 24 ± 1 °C. The largest number of adventitious shoots was obtained after 28 days exposure to 66 M BAP-supplemented modified Gresshoff and Doy 1 (GD1) medium. Relatively high frequencies of large shoots were obtained after a 14-day exposure to 22 M BAP-supplemented Brown and Lawrence (BL) or 66 M BAP-supplemented GD1. Adventitious shoots derived from 21- or 28-day exposures to BAP developed more slowly and were smaller in size than those derived from a 14-day exposure to the cytokinin. Shoot differentiation and subsequent growth were also influenced by basal media, media concentration, and presence of activated charcoal in the medium. The percentage of cotyledons forming shoots was highest on half-strength GD1 medium containing activated charcoal. Rooting was achieved in vitro under a continuous photoperiod of 60–70 mol M^–2 S^–1. Roots were formed when excised shoots were planted on GD 1/2 medium supplemented with 2.68 M 1^–1 a-napthaleneacetic acid (NAA) with or without BAP for 14 days. The proposed technique of slash pine propagation using cotyledon explants can produce up to 100 seedlings per embryo.     

Agrobacterium-mediated transformation of cork oak (<Emphasis Type="Italic">Quercus suber</Emphasis> L.) somatic embryos

A simple method is presented for the unreported genetic transformation of cork oak (Quercus suber L.). Pro-embryo masses (PEMs) were induced on immature zygotic embryos applied to medium supplemented with 2.3 M 2,4-dichlorophenoxyacetic acid. The established PEMs were inoculated with Agrobacterium tumefaciens LBA4404/p35S GUS INT/pCAMBIA 1301 strain. Transformants were selected on hygromycin (HYG) 94 M-supplemented medium. Viable embryos constituted 13% of those selected on HYG during 4 months. Expression of -glucuronidase at 4 months following co-cultivation confirmed transformation in 5.8% embryos selected on HYG. This method forms a basis for genetic transformation of cork oak somatic embryos.     

In vitro and ex vitro rooting of micropropagated shoots using three green ash (Fraxinus pennsylvanica) clones

Shoot multiplication using seedling materials was achieved by subculture on Murashige and Skoog salts with Gamborg's B5 vitamins (MSB5) medium containing a combination of 5 M 6-benzyladenine (BA), 5 M thidiazuron (TDZ), and 1 M indole-3-butyric acid (IBA) with three green ash (Fraxinus pennsylvanica) clones, SD1009 (South Dakota origin), SD2002 (South Dakota origin), and KA2018 (Kansas origin). Shoots were rooted using in vitro and ex vitro methods. For in vitro rooting studies, elongated shoots were transferred to rooting plugs supplied with liquid MSB5 medium containing a 3×3 factorial arrangement of two different auxins, -naphthaleneacetic acid (NAA) and IBA, at three concentrations (0, 5, and 10 M). The most effective treatment for in vitro root number, root length, and shoot height was 5 M IBA. The three clones also were tested for ex vitro rooting using a quick dip in 1 mM NAA, 1 mM IBA, or control (no auxin). The maximal ex vitro rooting response occurred when shoot explants of the three clones were dipped in 1 mM IBA. Significant clonal differences were noted in response to in vitro and ex vitro rooting treatments. Rooted plantlets were acclimated to the greenhouse.     

In vitro propagation of <Emphasis Type="Italic">Oroxylum indicum</Emphasis> Vent. a medicinally important forest tree

In vitro propagation of Oroxylum indicum Vent. was carried out using cotyledonary node explants. Among the different types of cytokinins used for culture establishment, 6-benzyladenine exhibited the best response with higher concentrations (8.87µM or above) for inducing multiple shoots. Inclusion of indole-3-acetic acid (2.85µM) into 6-benzyladenine-supplemented medium triggered a high frequency of response as well as a proliferation of shoots. The best medium for proliferation was Murashige-Skoog (MS) medium with 6-benzyladenine (8.87µM) and indole-3-acetic acid (2.85µM). However, incorporation of gibberellic acid (1.44µM) was mandatory to enhance shoot elongation. Repeated subculturing of cotyledonary node and in vitro developed nodal segments in MS medium with 6-benzyladenine (4.44µM) at 4-week intervals resulted in continuous mass multiplication of shoots without any evidence of decline. Root induction was best (91.6%) when MS strength was reduced to one-quarter and combined with -naphthalene acetic acid (2.69µM) and indole-3-acetic acid (5.71µM), with a high survival rate (70–72%) of plantlets hardened in either soil rite or soil:sand:soil rite (1:1:2).     

Slash pine (Pinus elliottii Engelm.) somatic embryogenesis II. Maturation of somatic embryos and plant regeneration

Maturation of slash pine (Pinus elliottii Engelm.) somatic embryos was achieved using two protocols, each starting with a different agar incubation step to deplete plant growth regulators (PGRs) used in previous cultural steps. Strength of maturation medium (single vs. double) was found important in the first protocol to develop normal, mature embryos. In the second protocol, abscisic acid (ABA) concentrations (0, 15 and 30 M) and carbohydrate sources were tested for embryo maturation. Thirty M ABA and 6% maltose were deemed the best combination. Embryo germination was accomplished in a continuously lighted environment and embryos receiving a cold pretreatment (4 °C in darkness for 16 days) germinated better than embryos which did not receive cold pretreatment. With a survival rate of 33% after acclimation in a mist system, more than 25 plants from somatic embryos have been established in a greenhouse. Incompletely germinated embryos (lacking roots) were rooted via adventitious rooting techniques and subsequently established in the greenhouse. All established plants obtained from somatic embryogenesis appear normal in morphology.     

Water circulation, groundwater outflow and nutrient dynamics in Mida creek, Kenya

The relationship between physical hydrodynamic processes and nutrients dynamics was investigated in Mida creek, a groundwater influenced mangrovefringed creek in Kenya between March 1996 and May 1997. The research involved spot and timeseries measurement of nitrate–nitrite, ammonia, silicates, phosphates, salinity, temperature, sealevel as well as tidal currents at seven stations located in the front, middle and backwater zones of the creek. Groundwater level as well as total dissolved solids' concentration, salinity, temperature and nutrients' concentration were also measured once every month in shallow wells (watertable<5m) located in the upper region of the creek. Results of the study show that nutrient concentrations vary with the tide and that, though there is no river drainage, they are of the same magnitude as in mangrove creeks with substantial river runoff. The peak concentrations of NH ₄ ⁺ –N (5.45M), NO ₂ –NO ₃ (5.63M), PO ₄ ^3– –P (0.58M) and SiO ₃ ^2– –Si (81.36M) in the creek occurred during flood tide, 2–3h before high waters. The (NO ₂ ^– + NO ₃ ^– )–N concentrations declined rapidly during ebb tide, reaching the minimum levels during low water. Contribution of groundwater seepage to the net nutrients flux (particularly on nitrite–nitrates) is largest in dry seasons. The study shows that groundwater outflow sustains the mangroves during periods of severe salinity stress and nutrients deficiency in dry seasons. This is essentially by limiting salinity increase and by boosting nutrient supply in dry seasons.     

Inhibition ofTrichoderma cellulase activity by a stilbene glucoside fromPicea glehnii bark

The stilbene glucoside isorhapontin (5,4-dihydroxy-3-methoxystilbene-3--D-glucoside) is the major constituent of the ethyl acetate extracts fromPicea glehnii bark. Isorhapontin inhibited the hydrolytic activity ofTrichoderma cellobiohydrolase I (CBH I) for both bacterial microcrystalline cellulose and the soluble cellooligosaccharide celloheptaitol. The inhibitory effect for celloheptaitol, however, was more drastic than that for bacterial microcrystalline cellulose. The hydrolytic activity of the CBH I core domain for celloheptaitol was also inhibited by isorhapontin to a similar extent, suggesting that the interaction between isorhapontin and the core domain of CBH I is the reason for this phenomenon. The inhibition of CBH I activity by isorhapontin showed mixed noncompetitive and uncompetitive types in a concentration of the inhibitor of less than 125M. TheK _i andK _i values were estimated to be 57.2 and 33.3M, respectively. Whereas isorhapontin strongly inhibited CBH I activity, its aglycone isorhapontigenin (3-methoxy-3,5,4-trihydroxystilbene) showed almost no inhibition. Consequently, both the stilbenic and the-glucosidic structures in isorhapontin are essential for the inhibitory effect on CBH I activity. Isorhapontin also inhibited the activity ofTrichoderma endoglucanase I for celloheptaitol, whereas almost no effect was observed for the activities of both endoglucanases II and III.An outline of this work was presented at the 49th Annual Meeting of the Japan Wood Research Society, Tokyo, April 1999     

Five heavy metals in propagules of ten mangrove species of China

Five heavy metals in the propagules of 10 mangrove species in China have been investigated. The results revealed that the levels of five heavy metals in most of the propagules were lower than the background levels of the soil. The levels of copper, manganese, zinc, cadmium, and lead in the propagules varied at about 2.1–7.8 g/g, 3.9–28.0 g/g, 5.7–60.0 g/g, 0.014–0.057 g/g, and 0.018–0.038 g/g, respectively. On average, the levels of five heavy metals were in the order Zn > Mn > Cu > Cd > Pb. The biological absorption coefficients were 0.02–1.30 for copper, manganese, zinc, and cadmium and about 0.0007–0.0020 for lead in propagules, showing the following order: Zn > Cd > Cu > Mn > Pb. The above results indicate that the five heavy metals have low accumulation in most of the propagules of 10 mangrove species and are at safe levels for the effective utilization of mangrove resources.     

Maximum effective lumen and pit pore sizes of the earlywood and the latewood of never dried loblolly pine sapwood

Summary Measurements of the air pressure required to initially displace a saturating liquid and allow a slow continuous stream of air bubbles to pass through wood cross sections of different thickness, together with the equilibrium surface tension of the saturating liquid, make it possible to calculate the maximum effective opening radii. Previous measurements were made for wood as a whole over complete annual rings. Measurements reported here were made separately for earlywood and latewood. Extrapolating plots of the maximum effective opening radius-cross section thickness, for thicknesses below the maximum fiber length, to zero thickness gave maximum lumen radii of 16 m for the earlywood and 10.3 m for the latewood. The values are only slightly greater than the calculated average values. Extrapolating the plots in the opposite direction to zero opening radius gave approximate maximum lumen or fiber lengths for the earlywood of 6 mm and for the latewood of 5 mm. The maximum effective opening radii for cross sections thicker than the maximum fiber length give maximum effective communicating pore radii. These values continue to decrease, with increasing thickness of the cross sections due to the decreasing probability of the largest openings falling in any one series path through the structures. The maximum effective pit pore radius for passage through fifty pits in series was 0.8 m for the earlywood and 0.28 m for the latewood.Paper No. 3787 of the Journal Series of North Carolina State University Agricultural Experiment Station, Releigh, N. C.     

Die Übertragung des Enationenvirus der Erbse durch verschiedene Morphen der BlattlausartenMacrosiphum euphorbiae (Thomas) undAcyrthosiphon pisum (Harris)

Zusammenfassung Unterschiedliche Vektoreignungen können zwischen Rassen, Morphen und Altersstadien einer Blattlausart auftreten. Es wurden verschiedene Morphen vonMacrosiphum euphorbiae (Thomas) undAcyrthosiphon pisum (Harris) hinsichtlich ihrer Übertragungsfähigkeit für das persistente Enationenvirus der Erbse geprüft. Dabei erwiesen sich ovipare Weibchen und adulte Männchen vonM. euphorbiae als ungeeignete Überträger, während die gleichen Morphen von zweiA. pisum-Populationen die Virose übertrugen. Auch die Fundatrix desA. pisum-BastardsTrifolium pratense rot XSarothamnus scoparius grün übertrug zu einem relativ hohen Prozentsatz. Ovipare Weibchen und Männchen der inaktiven RasseLotus uliginosus gelb konnten keine Infektion bewirken; die persistente Virusübertragungsfähigkeit ist im Falle vonAcyrthosiphon pisum (Harris) offenbar ein genetisch fixiertes Merkmal.

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Summary Different vector abilities may exist between races, morphs and development instars of a given aphid species. Several morphs ofMacrosiphum euphorbiae (Thomas) and ofAcyrthosiphon pisum (Harris) were tested in their transmission ability for the persistent pea enation mosaic virus. Oviparous females and adult males ofM. euphorbiae proved insuitable for transmission, the corresponding morphs of twoA. pisum-populations, however, were successful in transmitting. The fundatrix of theA. pisum hybridTrifolium pratense red xSarothamnus scoparius green transmitted to a relatively high percentage. Oviparous females and males of the inactiv raceLotus uliginosus yellow failed completely in transmitting; in the present case ofA. pisum the transmission character of the persistent virus evidently is genetically fixed.

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Résumé Différentes aptitudes de vecteur peuvent se trouver entre les races, les morphes et les périodes d'âge d'une espèce de pucerons. Différentes morphes deMacrosiphum euphorbiae (Thomas) et deAcyrthosiphon pisum (Harris) ont été examinées en ce qui concerne leurs aptitudes de transmettre le virus persistant du pois(pea enation mosaic). En ce cas les femelles ovipares et les mâles adultes deM. euphorbiae se montraient impropres à la transmission, pendant que les mêmes morphes de deux populations deA. pisum transmettaient la virose. C'était la fondatrice du bâtard deA. pisum Trifolium pratense rouge avecSarothamnus scoparius vert qui transmettait à une pourcentage relativement haute. Les femelles ovipares et les mâles de la race «inactive» deLotus uliginosus jaune ne pouvaient pas effectuer d'infection; en cas deAcyrthosiphon pisum (Harris), l'aptitude persistante à transmettre le virus évidemment est un caractère fixé d'une façon génétique.

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, . Macrosiphum euphorbiae (Thomas) Acyrthosiphon pisum (Harris) peaenation mosaic virus). M. euphorbiae , {A. pisum} . A. pisum-Trifolium pratense Sarothamnus scoparius . «» Lotus uliginosus ; Acrythosiphon pisum (Harris) .

     

A study on some anatomical parameters of the piercing-sucking process in leaves and branches of<Emphasis Type="Italic"> Pittosporum tobira</Emphasis> L. (Pittosporaceae) infested by the cottony cushion scale,<Emphasis Type="Italic"> Icerya purchasi</Emphasis> Maskell (Homoptera: Coccina,Margarodidae)

Pittosporum tobira L. (Family Pittosporaceae) is an important plant species grown in parks and gardens in Turkey. The cottony cushion scale, Icerya purchasi Maskell (Homoptera: Coccina, Margarodidae), is an important pest of P. tobira, and causes damage mainly to the leaves, branches and stems of the host plant. In spite of the great economic importance of I. purchasi to its host plant P. tobira, information has been limited on some basic anatomical parameters of the nature of the damage to leaves and branches. The present study was, therefore, initiated to investigate the insertion and penetration of stylets into the leaves and branches of P. tobira, the length of stylets and depth of penetration in both of these tissues, and penetration of stylets into the cortex, xylem and phloem tissues and into the secretory and non-secretory canals of the leaves and branches. The results showed great variations between the leaves and branches in all the criteria observed in I. purchasi infesting P. tobira. The study found 20 whole and 23 broken mouthparts in the leaves as compared to 3 whole mouthparts and 1 broken in the branches. Length of the stylets inserted into the leaves ranged from 33.83 m to 540.93 m and into the branches from 202.85 m to 340.8 m. The stylets reached greater depths in the leaves, at 540.93 m, as compared to 498.67 m in the branches. The stylets in the leaves were associated with 12 secretory canals, but with none in the branches. It was found that three stylets reached xylem in the leaves as compared to one in the branches. Similarly, 17 stylets in the leaves reached to the phloem, but only 2 in the branches. No stylets were found in the cortex of the leaves, but one was found in the branches. In the leaves, 11 stylets reached and remained in the non-secretory canals, but none in the case of the branches.     

Field measurements of gas exchange in Avicennia marina and Bruguiera gymnorrhiza

Diurnal gas exchange characteristics were measured simultaneously in two mangrove species, Avicennia marina and Bruguiera gymnorrhiza, over 7 d in summer (February–March), to compare their productivity. The study was undertaken in the Beachwood Mangroves Nature Reserve, Durban, South Africa, using fully expanded leaves of young and mature trees at the top of the canopy. Gas exchange was strongly influenced by photosynthetic photon flux density (PPFD), leaf temperature and the accompanying leaf to air vapour pressure deficit ( w). Carbon dioxide exchange was saturated at a PPFD of about 600 mol m^-2s^-1 in B. gymnorrhiza compared to 800 mol m^-2s^-1 in A. marina. Maximal CO₂ exchange occurred between 12h00 and 14h00 and was consistently greater in A. marina (8.8 mol m^-2s^-1) than in B. gymnorrhiza (5.3 mu;mol m^-2s^-1). Mean internal CO₂ concentrations ( c_i) were 260 l l^-1 in A. marina and 252 l l^-1 in B. gymnorrhiza. Photorespiratory activity was 32% in A. marina and 30% in B. gymnorrhiza. Mean water use efficiency (WUE) was 8.0 mol mmol^-1 in A. marina and 10.6 mol mmol^-1 in B. gymnorrhiza. Diurnal leaf water potentials ranged from –0.8 to –3.5 MPa and were generally lower in A. marina.     

Absolute configuration of arylglycerol-β-aryl ethers obtained by asymmetric reduction of the correspondingα-ketonic compound with intactFusarium solani cells

When (±)--oxo-guaiacylglycerol--(vanillic acid) ether (1) is degraded byFusarium solani M-13-1, the-ketone is initially reduced to giveerythro andthreo guaiacylglycerol--(vanillic acid) ethers (2), arylglycerol--aryl ethers, both of which are enantiomerically pure. The absolute configuration in each2 was determined by Mosher's method; the products were converted to,-di-(R)--methoxy--trifluoromethylphenylacetates (MTPA esters) (3) oferythro (-)- andthreo (+)-veratrylglycerol--(methyl vanillate) ethers (3), whose¹H nuclear magnetic resonance (NMR) spectra were examined and compared with those of four di-(R)-MTPA ester (3) diastereomers from chemically synthesizederythro (±)-3 andthreo (±)-3. To assign the- and-MTPA-OCH₃ peaks, the¹H NMR scans of several compounds that have substructures of 3 and their 3,4,5-trimethoxyphenyl analogues were examined. When a racemic alcohol reacts with (R)-MTPA to give a pair of (R)-MTPA ester diastereomers, the value was defined as the absolute value of the difference in the¹H chemical shifts of the peak between the diastereomers. It was found that the values of-MTPA-OCH₃ were larger than those of-MTPA-OCH₃ owing to a shielding effect of the veratryl ring located on the-MTPA-OCH₃, and that the-MTPA-OCH₃ peaks in the 3,4,5-trimethoxyphenyl compounds shifted downfield relative to those in the veratryl compounds. On the basis of the¹h NMR data of (R)-MTPA esters, the absolute configuration of the four chemically prepared diastereomers (3) were determined. The catabolicerythro 3 [fromerythro (-)-3] andthreo 3 [fromthreo (+)-3] were identical to (R, S, R)-erythro 3 and (R, S, S)- threo 3, respectively. An hydrogen species in the fungal reduction would attack the-ketone fromre-face of both (R)-1 and (S)-1, givingerythro (S, R)-2 andthreo (S, S)-2, respectively.Part of this paper was presented at the 33rd Lignin Symposium, Tsukuba, November 1988     

Ultrastructure of pits inPinus banksiana lamb

Summary The cross-sectional view of pitting between various cell types inPinus banksiana Lamb. was studied at the ultrastructural level. Cell types inPinus banksiana include longitudinal tracheids, ray tracheids, ray parenchyma cells, buffer cells and epithelial cells. Two common characteristic features of bordered pit-pairs between longitudinal tracheids are an initial pit border and a thickened torus at the center of the pit membrane. The shape and size of the pit border and torus of bordered pit-pairs between two compression wood cells, and between the last-formed latewood longitudinal tracheid and first-formed earlywood longitudinal tracheid were different from those in the earlywood and latewood longitudinal tracheids. The pit border on the ray tracheid side varied in size and shape due to wall dentation. No initial pit border was found on the pit border of the ray tracheid side. The shape of bordered pit-pairs between two ray tracheids varied considerably due to irregularity of the dentate cell wall. The size of bordered pit-pairs in longitudinal tracheids was between 16 m to 20 m, which was twice the diameter of bordered pit-pairs in ray tracheids. Bordered pitpairs at the end wall of two ray tracheids appeared to be the smallest at 5 m, Pit aspiration occurred in the bordered pit-pairs with or without a torus. In the heartwood zone, some half-borders pit-pairs between tracheary and ray parenchyma cells showed an additional secondary wall on the ray parenchyma cell side. Plasmodesmata were found in the half-bordered pit-pairs as well in the simple pit-pairs. Blind pits were observed between a ray tracheid and a longitudinal tracheid. Bordered pit-pairs between two buffer cells were also observed. The possible functions of buffer cells were discussed.Use of transmission electron microscope provided by the Science Instrumentation Lab, Lakehead University and the technical assistance provided by Mr. A. MacKenzie, Director of Science Instrumentation Lab are gratefully appreciated     

Stock quality and field performance of Pinus patula seedlings produced under two nursery growing regimes during seven different nursery production periods

Two types of containerized P. patula seedlings, referred to as hard and soft seedlings, were raised by droughting and different fertilizer applications. Hard seedlings were raised by applying half the water and a quarter of the fertilizer (0.01 g per seedling 21%N:7.1%P:14.2%K) applied to the soft seedlings. At the end of the nursery cultural treatments the two types of seedlings were morphologically distinct. Hard seedlings had lower heights, height to root collar diameter ratios and seedling dry masses than soft seedlings, but mortality was similar for both seedling types when planted during seven different production periods within the planting season on three sites which differed climatically and geologically. Correlations between mortality and height to root collar diameter ratio (r = 0.76), root to shoot ratio (r = 0.83) and root growth potential (r = --0.69) were obtained indicating that grading of seedlings on these parameters may improve survival. However, it was evident that survival could be significantly improved by identifying the best time of year and conditions for planting, as well as improving stock quality.     

Commercial micropropagation of five Alnus species

Clonal micropropagation was demonstrated as feasible on a commercial basis for several clones of five Alnus species. Approximately 60,000 ready-to-root individual shoots were multiplied in vitro on modified MS medium supplemented with 2.5–5 M BAP. A total of 15,500 shoots from different clones were rooted in vitro on half strength MS medium including 1–5 M IBA. They were transferred under mist conditions within a growth chamber illuminated with high pressure sodium lamps. Those conditions gave 99–100% plantlet survival after four weeks. Plantlets were then inoculated with selected Frankia sp. strains. These nodulated alder plants are under field evaluation at the Petawawa National Forestry Institute, Canadian Forestry Service in Chalk River, Ontario.     

Neuere Beobachtungen und Untersuchungen zur Kartierung von Pflanzenschädlingen

Summary In the introduction a historical survey is given about the development of the registration service of pests. It can be seen that originally informations of the appearance of pests was mostly given by agricultural and horticultural experts in special journals of agriculture and horticulture.It is well known that a registration service was set up when a Plant Protection Organisation was foundet. Its efficiency has never ceased since its beginnings. These efforts are foundet intensionally to register the existence of pests as completely as possible.The registration service starting first of all from the damage caused by the pests maybe well understood.Our research work being a continuous contribution to the systematic registration of pests generally shows in a number of examples the heterogeneous character of the problem which must be studied concerning the individual pests and difficulties which arise. This is shown with regard to someElaterides and also because of experiments withLaspeyresia funebrana Tr. during several years.In accordance withKirchner and F. P.Müller, Rostock, we propose to register systematically a number of pests on index cards according to uniform principles in the whole of Europe, to register the vertical and horizontal appearance of pests with the same measure, in the same periods of classification and in the same way (seeAuersch 1 to 3).We are encouraged to do so by a number of appreciated views of specialists from Germany and foreign countries however it is, not enaugh to see the great importance of the card index (card-register) and to acknowledge its practicability, but it can only prove its efficiency if it is generally introduced.The systematic registration of pests will enable us to gather in the course of time empirical data, the reliability and evidence of which will steadily increase.

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Résumé On commence par livrer un aperçu historique sur le développement du système de rendre compte relativement aux plantes nuisibles. En lisant ces comptes rendus on peut reconnaître que les rapports donnés au début sur l'apparition des plantes nuisibles furent livrés en substance par la pratique agricole et jardinière par des revues spéciales de l' agriculture et de l'horticulture.Il est connu qu'avec l'établissement du service pour la protection des plantes on a établi un service de comptes rendus, au perfectionnement duquel on a travaillé dès son existance.Ces efforts furent influencés et activés par l'idée de saisir l'apparition des plantes nuisibles aussi complétement que possible. Le service des comptes rendus s'est orienté d'abord en première ligne aux dommages produits, ce qui est aisé à comprendre.Puisque notre traité doit être en général une contribution ultérieure à la connaissance systématique de l' apparition des plantes nuisibles, on explique au moyen de plusieurs exemples, comment sont différentes les questions se rapportant aux plantes nuisibles individuelles, questions qu'il faut ainsi prendre en considération et quelles difficultés en résultent. Cela est démontré pour quelques élatérides de même que sur la base d'examinations exécutées pendant plusieurs années avecLaspeyresia funebrana Tr.En concordance avecKirchner et F. P.Müller, Rostock, nous proposons de saisir systématiquement en classeurs à fiches une série de plantes nuisibles selon des points de vue uniformes, cela veut dire d'enregistrer dans les fiches proposées l'apparition verticale et horizontale des plantes nuisibles avec des mesures égales, dans les mêmes périodes d'examen et appréciation des qualités productives du sol et dans la même manière (voireAuersch 1 à 3).Nous sommes encouragés à faire ces propositions avant tout par des assentiments à notre disposition, qui nous ont été envoyés de la part des cercles compétents de notre pays et de l'é tranger. Il ne suffit pas cependant de reconnaître le fichier dans sa signification variée et d'apprécier son utilisation; le fichier peut faire ses preuves plutôt seulement, s'il est introduit en général. Au cours du temps il sera possible d'obtenir des valeurs expérimentelles par le fichement systématique des plantes nuisibles, dont la sûreté et faculté de déposition augmente constammant avec la durée de leur existence.

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õ . : . . . . õ . . , . Elateriden uLaspeyresia funebrana Tr. . . . , , , . . ( 1 3.) . . , . . .

     

Mangrove forest productivity and biomass accumulation in Hinchinbrook Channel,Australia

Data on stand structure and rates of photosynthesis were used to estimate net canopy carbon fixation and carbon accumulation as living biomass in mangrove forests in Hinchinbrook Channel, Australia. Total annual canopy net carbon fixation was estimated to be about 29tCha–1yr–1. This equates to about 204,000tCyr–1 for all mangrove forests in Hinchinbrook Channel. Of this, only about 12% was stored as living plant biomass. Although it is not yet possible to present a robust carbon balance for mangrove trees, the remainder is presumably lost through plant respiration, litter fall, root turnover and exudation of organic compounds from roots.     

Structure of xylan from culms of bamboo grass (Sasa senanensis Rehd.)

Xylan prepared from culms of kumaizasa (Sasa senanensis Rehd.), a representative species of bamboo grass, was hydrolyzed with-xylanase ofStreptomyces olivaceoviridis E-86. Four arabinoxylo-oligosaccharides and two glucuronoxylo-oligosaccharides were isolated from the enzymatic hydrolysate of the xylan by chromatography on a charcoal column, a Dowex 1-x8 column, a Toyo-pearl HW-40S column, and a LiChrospher 100 NH₂ column and on preparative paper chromatography. The results of the structural analyses of the saccharides showed that the isolated oligosaccharides had the structures of 3²--l-arabinofuranosyl-xylobiose, 3²--l-arabinofuranosyl-xylotriose, 3²--[-d-xylopyranosyl-(1 2)-l-arabinofuranosyl]-xylobiose, 3³--[-d-xylopyranosyl-(1 2)-l-arabinofuranosyl]-xylotriose, 2³--4-O-methyl-d-glucuronosyl-xylotriose, and 2³--d-glucuronosyl-xylotriose. From the structural analysis of the oligosaccharides derived from the xylan, kumaizasa xylan was concluded to be a kind of arabinoglucuronoxylan having not only stubs of singlel-arabinose and singled-glucuronic acid but also stubs of disaccharide units such as-d-xylopyranosyl-(1 2)-l-arabinofuranose.