N<Subscript>2</Subscript> fixation by faba bean (<Emphasis Type="Italic">Vicia faba</Emphasis> L.) in a gypsum-amended sodic soil

The response of faba bean to the application of four rates of gypsum (0, 2.5, 5.0, 10.0 t ha⁻¹) to a non-saline, alkaline sodic soil was measured in terms of grain yield, dry matter (DM) production, N accumulation and the proportional dependence of the legume on symbiotic N₂ fixation (P _atm). A yield-independent, time-integrated ¹⁵N-dilution model was used to estimate symbiotic dependence. A significant decrease in the exchangeable sodium percentage and significant increases in exchangeable Ca⁺⁺ and the Ca⁺⁺:Mg⁺⁺ ratio in the 0–10-cm soil layer were measured 30 months after application of 10 t ha⁻¹ gypsum. Despite low and erratic rainfall during crop growth, faba bean DM and N uptake responded positively to gypsum application. The symbiotic dependence of the legume at physiological maturity was little affected by sodicity (P _atm = 0.74 at zero gypsum and 0.81–0.82 at 2.5–10 t ha⁻¹ gypsum). The increase in fixed N due to gypsum application was mainly due to increases in legume DM and total N uptake. At 10 t ha⁻¹ of gypsum, faba bean fixed more than 200 kg N ha⁻¹ in above-ground biomass.     

Wheat response to N2 fixed by faba bean (Vicia faba L.) as affected by sulfur fertilization and rhizobial inoculation in semi‐arid Northern Ethiopia

Nitrogen fixation in faba bean (Vicia faba cv. Mesay) as affected by sulfur (S) fertilization (30 kg S ha^–1) and inoculation under the semi‐arid conditions of Ethiopia was studied using the ¹⁵N‐isotope dilution method. The effect of faba bean–fixed nitrogen (N) on yield of the subsequent wheat crop (Triticum aestivum L.) was also assessed. Sulfur fertilization and inoculation significantly (p < 0.05) affected nodulation at late flowering stage for both 2004 and 2005 cropping seasons. The nodule number and nodule fresh weighs were increased by 53% and 95%, relative to the control. Similarly, both treatments (S fertilization and inoculants) significantly improved biomass and grain yield of faba bean on average by 2.2 and 1.2 Mg ha^–1. This corresponds to 37% and 50% increases, respectively, relative to the control. Total N and S uptake of grains was significantly higher by 59.6 and 3.3 kg ha^–1, which are 76% and 66% increases, respectively. Sulfur and inoculation enhanced the percentage of N derived from the atmosphere in the whole plant of faba bean from 51% to 73%. This corresponds to N₂ fixation varying from 49 to 147 kg N ha^–1. The percentage of N derived from fertilizer (%Ndff) and soil (%Ndfs) of faba bean varied from 4.3% to 2.8 %, and from 45.1% to 24.0%, corresponding to the average values of 5.1 and 47.9 kg N ha^–1. Similarly, the %Ndff and %Ndfs of the reference crop, barley, varied from 8.5 % to 10.8% and from 91.5% to 89.2%, with average N yields of 9.2 and 84.3 kg N ha^–1. Soil N balance after faba bean ranged from 13 to 52 kg N ha^–1. Beneficial effects of faba bean on yield of a wheat crop grown after faba bean were highly significant, increasing the average grain and N yields of this crop by 1.11 Mg ha^–1 and 30 kg ha^–1, relative to the yield of wheat grown after the reference crop, barley. Thus, it can be concluded that faba bean can be grown as an alternative crop to fallow, benefiting farmers economically and increasing the soil fertility.     

Dinitrogen fixation activity of Azorhizobium caulinodans in the rice (Oryza sativa L.) rhizosphere assessed by nitrogen balance and nitrogen-15 dilution methods

 Azorhizobium caulinodans was directly inoculated onto rice plants in three short-term pot trials. Addition of increasing amounts of sucrose (23, 46, 92 kg ha^–1) did not influence the N economy of the A. caulinodans-rice association during the early vegetative growth stage. A. caulinodans inoculation alone and in combination with the highest amount of sucrose had a significantly positive effect on the N balance, with small but significant N gains in the system. Application of 60 kg urea-N ha^–1 had a negative impact on the N economy of the inoculated treatments. N losses increased and the amount of atmospheric N₂ fixed and incorporated decreased significantly as compared to the amounts under the 20 kg urea-N ha^–1 regime. However, N losses were low – a maximum of 8% – at the early vegetative growth stage under the conditions of the experiments. C limitation does not seem to be a limiting factor for the incorporation of fixed N₂ in this bacteria-plant association. Biological N₂ fixation caused by A. caulinodans inoculation was responsible for 14% of the plant N at the vegetative growth stage and under low N conditions. Received: 30 January 2000     

Response of Mucuna pruriens to symbiotic nitrogen fixation by rhizobia following inoculation in farmers' fields in the derived savanna of Benin

 Leguminous cover crops such as Mucuna pruriens (mucuna) have the potential to contribute to soil N and increase the yields of subsequent or associated cereal crops through symbiotic N fixation. It has often been assumed that mucuna will freely nodulate, fix N₂ and therefore contribute to soil N. However, results of recent work have indicated mucuna's failure to nodulate in some farmers' fields in the derived savanna in Benin. One of the management practices that can help to improve mucuna establishment and growth is the use of rhizobial inocula to ensure compatibility between the symbiotic partners. Experiments were conducted in 1995 and 1996 on 15 farmers' fields located in three different villages (Eglimé, Zouzouvou and Tchi) in the derived savanna in Benin. The aim was to determine the response of mucuna to inoculation and examine the factors affecting it when grown in relay cropping with maize. The actual amount of N₂ fixed by mucuna in the farmers' fields at 20 weeks after planting (WAP) averaged 60 kg N ha^–1 (range: 41–76 kg N ha^–1) representing 55% (range: 49–58%) of the plant total N. The result suggested that mucuna in these farmers' fields could not meet its total N demand for growth and seed production only by N₂ fixation. It was estimated that after grain removal mucuna led to a net N contribution ranging from –37 to 30 kg N ha^–1. Shoot dry weight at 20 WAP varied between 1.5 and 8.7 t ha^–1 and N accumulation ranged from 22 to 193 kg N ha^–1. Inoculation increased shoot dry matter by an average of 28% above the uninoculated treatments, but the increase depended on the field, location and year. For the combinations of inoculated treatments and farmers' fields, the response frequency was higher in Eglimé and Tchi than in Zouzouvou. The response to inoculated treatments was dependent on the field and inversely related to the numbers of rhizobia in the soil. Soil rhizobial populations ranged from 0 to >188 cells g^–1 soil, and response to inoculation often occurred when numbers of indigenous rhizobia were <5 cells g^–1 soil. In two farmers' fields at Zouzouvou where extractable P was below 10 μg g^–1 soil, mucuna did not respond to rhizobial inoculation despite a higher population of rhizobia. Significant relationships between mycorrhizal colonization, growth and nodulation of mucuna were observed, and inoculated plants with rhizobia had a higher rate of colonization by arbuscular mycorrhizal fungi (%AMF) than uninoculated ones. Therefore, it was shown that mucuna will establish and fix N₂ effectively in those fields where farmer's management practices such as good crop rotation and rhizobial inoculation allow a build up of AMF spores that might lead to a high degree of AMF infection and alleviate P deficiency. Received: 14 June 1999     

Quality,yield and nitrogen fixation of faba bean seeds as affected by sulphur fertilization

Abstract

Faba bean (Vicia faba L.) is an important source of plant protein for humans and animals; however, nutritional value of seeds is notoriously deficient in sulphur (S)-containing amino acids. In this article, the effect of S fertilization on faba bean's capability of N₂ fixation, grain yield and chemical characteristics in terms of protein fractions, fatty acids and minerals composition is reported. A randomized, complete block design with three replicates was used, and three S applications (0, 30 and 60 kg ha^?1, respectively) for faba bean were performed. The S fertilization was split into two applications: 50% before sowing and 50% in the beginning of March as K₂SO₄. At the same time, both the legume and oat crops were fertilized uniformly with 10 kg N ha^?1 as ¹⁵N NH₄ ¹⁵NO₃ (10% ¹⁵N atomic excess) in solution form. In a Mediterranean climate under optimal spring rainfall situations, faba bean produced high yield of grain and protein. Sulphur application resulted in an increase in overall plant yield and N₂ fixation. In addition, S fertilization enhanced the protein quality, increasing its degradable fraction. Fertilizing faba bean with 30 kg ha^?1 of S resulted in a more appropriate dose in order to obtain a quantitative and qualitative crop improvement. From our findings, it can be concluded that S fertilization to faba bean should be recommended to soils with suboptimal S levels to obtain maximum seed and protein yields.     

Effects of intercropping and Rhizobium inoculation on yield and rhizosphere bacterial community of faba bean (Vicia faba L.)

The effects of intercropping with maize and Rhizobium inoculation on the yield of faba bean and rhizosphere bacterial diversity were analyzed by terminal restriction fragment length polymorphism, amplified 16S rDNA restriction analysis (ARDRA), and 16S rDNA sequencing. The results showed that intercropping but not Rhizobium inoculation significantly increased the faba bean yield. Probably the relatively high level of native rhizobia in soil annulled the effect of rhizobia inoculation. ARDRA results showed that intercropping did not affect bacterial diversity whereas Rhizobium inoculation decreased bacterial diversity. The canonical correspondence analysis showed that the composition of bacterial community was changed apparently by intercropping, and there was a positive correlation (P = 0.724) between faba bean yields and intercropping and an apparent correlation (P = 0.648) between intercropping and total N. The available content of K and P had a lower effect on the bacterial community composition than did the total N content, Rhizobium inoculation, and microbial biomass C. Rhizobium inoculation negatively correlated with microbial biomass C (P = −0.827). These results revealed a complex interaction among the intercropped crops, inoculation with rhizobia, and indigenous bacteria and implied that the increase of faba bean production in intercropping might be related to the modification of rhizosphere bacterial community.     

Field evaluation of lentil cultivars inoculated with Rhizobium leguminosarum bv. viciae strains for nitrogen fixation using nitrogen-15 isotope dilution

 A ¹⁵N isotope dilution technique was applied to quantify the extent of N₂ fixation in lentil (Lens culinaris Medik.) cultivars as influenced by Rhizobium leguminosarum bv. viciae strains in a field experiment in Pakistan. The experiment was conducted on a soil with a very small indigenous rhizobial population and where N was a limiting factor for crop production. Significant variations in number of nodules, dry weight of nodules, biomass yield, grain yield, total N yield, proportion of plant N derived from N₂ fixation (P_fix) and amount of N derived from the atmosphere (N_dfa) were observed among combined treatments of four rhizobial strains and six lentil varieties. In a field previously labelled with ¹⁵N, to which a basal dose of 75 kg P₂O₅ ha^–1 was applied as single super phosphate, N_dfa ranged from 15 to 24 kg N ha^–1 when calculated according to rhizobial strain and from 4 to 38 kg N ha^–1 when calculated according to lentil variety. Lc 26 was the most effective strain and fixed 243% more N than the indigenous population in the uninoculated control. In treatments with the lentil variety PL-406, N_dfa was 38 kg N ha^–1, which was 850% higher than with the lentil variety Precoz/F6-20-1×M-85. Generally, the varieties with greater P_fix produced a higher dry matter yield. Received: 26 May 1999     

Nodulation and N2 fixation of faba bean (Vicia faba L.) after soil amendment with crop residues

The effect of prior soil amendment with different N sources at 50 mg N (kg soil)^—1 on nodulation and N₂ fixation of faba bean (Vicia faba L. cv. Troy) using wheat (Triticum aestivum L. cv. Star) as reference crop was assessed in a pot experiment. Four treatments viz legume manure (LEGM) as clover shoots, cereal manure (CEREM) as barley straw, N fertilizer (FERT‐N) as Ca(NO₃)₂, and no‐manure control (NOMAN) were investigated consecutively at 45, 70, and 90 days after sowing (DAS). Faba bean nodulated profusely, with an increase on average from 629 nodules per pot at 45 DAS to nearly 2.3‐ and 3.3‐fold at 70 and 90 DAS, respectively. Low nodule numbers and nodule dry matter occurred under FERT‐N and CEREM, whereas high values were found for NOMAN and LEGM. Soil amendment affected percent N₂ fixation in relation to N source and plant age. Highest percent N₂ fixation (≥ 90 %) was found under the lowest N‐supplying amendments, no‐manure, and cereal manure, respectively. FERT‐N depressed N₂ fixation particularly at 45 DAS when N₂ fixation was reduced to as low as 23 %. The rise in N₂ fixation thereafter suggests that faba bean adjusted after depletion of mineral N in the soil. N₂ fixation was also decreased after cereal straw application, even though N concentration in faba bean plants was high. The results indicate that plant residues, both with high and low N concentration, applied to soil to raise its fertility may interfere with N₂ fixation of faba bean.     

Non-isotopic method for the quantification of biological nitrogen fixation and wheat production under field conditions

 A field experiment was conducted in the Inshas area (Sharkeia governorate) to study the potential of biofertilizers, when the quantities of commercial fertilizers were reduced, for optimal wheat production. The different treatments were arranged in a completely randomized block design with seven replicates. N fertilizer was applied in three treatments with one control, i.e. zero, full, half and one-fourth rates, in the presence or absence of inocula. Azospirillum brasilense strain Sp245 was used as a biofertilizer. Generally, inoculation increased the accumulation of shoot dry matter and grain yield by about 35%, relative to the control treatment. Similar trends were observed in the case of N and P uptake by shoots and grains, as well as the efficient use of both, where inoculation increased the acquisition of the two elements as compared with the uninoculated plants. The obtained data showed that N₂ fixed by shoots and grains ranged from 2 to 10 kg N ha^–1 and from 8 to 19 kg N ha^–1, respectively. With respect to fixed N₂, the best treatment was inoculation combined with the one-fourth dose of N, followed by inoculation combined with half of the recommended N dose. Most of the fixed N was utilized by grains and the results clearly reflected the negative effect of high N fertilizer rates on biological N fixation. It is obvious that inoculation, in general, enhanced the N fertilizer utilized by both shoots and grains of wheat plants. In conclusion, the application of biofertilization technology to a light-textured soil with low fertility had a positive effect on plant growth, N gained from the air and enhancement of fertilizer N uptake (apparent recovery fraction). Received: 22 April 1999     

Long-term winter cover cropping effects on corn (Zea mays L.) production and soil nitrogen availability

 This study was conducted to determine effects of long-term winter cover cropping with hairy vetch, cereal rye and annual ryegrass on soil N availability and corn productivity. From 1987 to 1995, with the exception of the first year of the study, the cover crops were seeded each year in late September or early October after the corn harvest and incorporated into the soil in late April or early May. Corn was seeded 10 days to 2 weeks after the cover crop residues had been incorporated, and N fertilizer was applied as a side-dressing at rates of 0, 67, 134, or 201 kg N ha^–1 each year. While the average annual total N input from the above-ground biomass of the cover crops was highest for hairy vetch (72.4 kg N ha^–1), the average annual total C input was highest for cereal rye (1043 kg C ha^–1) compared with the other cover crops. Hairy vetch was the only cover crop that significantly increased pre-side-dressed NO₃ ^–-N (N_i) corn biomass and N uptake at 0 N. At an N fertilizer rate of 134 kg N ha^–1 or higher, the cover crops had a minimal effect on corn biomass. This indicated that even after 9 years of winter cover cropping, the effect of the cover crops on corn growth resulted primarily from their influence on soil N availability. The amount of available N estimated from the cover crops (N_ac) was significantly correlated with relative corn biomass production (r ²=0.707, P<0.001). The total amount of available N, comprising N_ac and N added from fertilizer (N_f), was strongly correlated (r ²=0.820, P<0.001)) with relative corn biomass production. The correlation was also high for the available N comprising N_i and N_f (r ²=0.775, P<0.001). Although cereal rye and annual ryegrass did not improve corn biomass production in the short term, they benefited soil organic N accumulation and gradually improved corn biomass production compared with the control over the long term. Received: 10 August 1999     

Effect of cropping systems on nitrogen mineralization in soils

 Understanding the effect of cropping systems on N mineralization in soils is crucial for a better assessment of N fertilizer requirements of crops in order to minimize nitrate contamination of surface and groundwater resources. The effects of crop rotations and N fertilization on N mineralization were studied in soils from two long-term field experiments at the Northeast Research Center and the Clarion-Webster Research Center in Iowa that were initiated in 1979 and 1954, respectively. Surface soil samples were taken in 1996 from plots of corn (Zea mays L.), soybean (Glycine max (L.) Merr.), oats (Avena sativa L.), or meadow (alfalfa) (Medicago sativa L.) that had received 0 or 180 kg N ha^–1 before corn and an annual application of 20 kg P and 56 kg K ha^–1. N mineralization was studied in leaching columns under aerobic conditions at 30  °C for 24 weeks. The results showed that N mineralization was affected by cover crop at the time of sampling. Continuous soybean decreased, whereas inclusion of meadow increased, the amount of cumulative N mineralized. The mineralizable N pool (N _o) varied considerably among the soil samples studied, ranging from 137 mg N kg^–1 soil under continuous soybean to >500 mg N kg^–1 soil under meadow-based rotations, sampled in meadow. The results suggest that the N _o and/or organic N in soils under meadow-based cropping systems contained a higher proportion of active N fractions. Received: 10 February 1999     

Phosphorus uptake and rhizosphere properties of intercropped and monocropped maize, faba bean, and white lupin in acidic soil

Little information is available on phosphorus (P) uptake and rhizosphere processes in maize (Zea mays L.), faba bean (Vicia faba L.), and white lupin (Lupinus albus L.) when intercropped or grown alone in acidic soil. We studied P uptake and soil pH, carboxylate concentration, and microbial community structure in the rhizosphere of maize, faba bean, and white lupin in an acidic soil with 0–250 mg P (kg⁻¹ soil) as KH₂PO₄ (KP) or FePO₄ (FeP) with species grown alone or intercropped. All plant species increased the pH compared to unplanted control, particularly faba bean. High KP supply (>100 mg P kg⁻¹) significantly increased carboxylate concentration in the rhizosphere of maize. The carboxylate composition of the rhizosphere soil of maize and white lupin was significantly affected by P form (KP or FeP), whereas, this was not the case for faba bean. In maize, the carboxylate composition of the rhizosphere soil differed significantly between intercropping and monocropping. Yield and P uptake were similar in monocropping and intercropping. Monocropped faba bean had a greater concentration of phospholipid fatty acids in the rhizosphere than that in intercropping. Intercropping changed the microbial community structure in faba bean but not in the other corps. The results show that P supply and P form, as well as intercropping can affect carboxylate concentration and microbial community composition in the rhizosphere, but that the effect is plant species-specific. In contrast to previous studies in alkaline soils, intercropping of maize with legumes did not result in increased maize growth suggesting that the legumes did not increase P availability to maize in this acidic soil.     

Growth,P uptake in grain legumes and changes in rhizosphere soil P pools

In soils with low P availability, several legumes have been shown to mobilise less labile P pools and a greater capacity to take up P than cereals. But there is little information about the size of various soil P pools in the rhizosphere of legumes in soil fertilised with P although P fertiliser is often added to legumes to improve N₂ fixation. The aim of this study was to compare the growth, P uptake and the changes in rhizosphere soil P pools in five grain legumes in a soil with added P. Nodulated chickpea (Cicer arietinum L.), faba bean (Vicia faba L.), white lupin (Lupinus albus L.), yellow lupin (Lupinus luteus L.) and narrow-leafed lupin (Lupinus angustifolius L.) were grown in a loamy sand soil low in available P to which 80 mg P kg⁻¹ was added and harvested at flowering and maturity. At maturity, growth and P uptake decreased in the following order: faba bean > chickpea > narrow-leafed lupin > yellow lupin > white lupin. Compared to the unplanted soil, the depletion of labile P pools (resin P and NaHCO₃-P inorganic) was greatest in the rhizosphere of faba bean (54% and 39%). Of the less labile P pools, NaOH-P inorganic was depleted in the rhizosphere of faba bean while NaOH-P organic and residual P were most strongly depleted in the rhizosphere of white lupin. The results suggest that even in the presence of labile P, less labile P pools may be depleted in the rhizosphere of some legumes.     

Effect of starter fertilizer in seed-row on emergence,biomass and nutrient uptake by six pulse crops grown under controlled environment conditions

Abstract

Legumes have a unique ability to obtain a significant portion of atmospheric nitrogen (N₂) through a symbiotic relationship with Rhizobia spp of bacteria but it takes time, thus, an early supply of N to the plant may positively influence growth and development. However, too much fertilizer in close proximity to the seed can damage the seedling. Therefore, this study was conducted to determine the maximum safe rates for starter seed-row fertilizer application under low seedbed utilization conditions (15%). Emergence, biomass yield and nitrogen (N), phosphorus (P) and sulfur (S) uptake responses to starter fertilizer products and blends applied at 0, 10, 20 and 30?kg?N?ha^?1 in the seed-row were investigated for six different pulse crops: soybean, pea, faba bean, black bean, lentil and chickpea. The general sensitivity (injury potential) for starter N, P, S fertilizer was lentil?≥?pea?≥?chickpea?>?soybean?≥?black bean?>?faba bean. Lentil, pea and chickpea could generally only tolerate the 10?kg?N?ha^?1 rates while soybean and black bean could tolerate 10–20?kg?N?ha^?1. Faba bean emergence appeared relatively unaffected by all three rates of N and showed least sensitivity to seed row placed fertilizer. In terms of 30-day biomass response, soybean and black bean were most responsive to fertilization, while pea, faba bean, lentil and chickpea were least responsive to the starter fertilizer applications, with no benefit increasing above the 10?kg?N?ha^?1 rate.     

Influence of nitrogen on cellulose and lignin mineralization in blackwater and redwater forested wetland soils

 Microcosms were used to determine the influence of N additions on active bacterial and active fungal biomass, cellulose degradation and lignin degradation at 5, 10 and 15 weeks in soils from blackwater and redwater wetlands in the northern Florida panhandle. Blackwater streams contain a high dissolved organic C concentration which imparts a dark color to the water and contain low concentrations of nutrients. Redwater streams contain high concentrations of suspended clays and inorganic nutrients, such as N and P, compared to blackwater streams. Active bacterial and fungal biomass was determined by direct microscopy; cellulose and lignin degradation were measured radiometrically. The experimental design was a randomized block. Treatments were: soil type (blackwater or redwater forested wetlands) and N additions (soils amended with the equivalent of 0, 200 or 400 kg N ha^–1 as NH₄NO₃). Redwater soils contained higher concentrations of C, total N, P, K, Ca, Mn, Fe, B and Zn than blackwater soils. After N addition and 15 weeks of incubation, the active bacterial biomass in redwater soils was lower than in blackwater soils; the active bacterial biomass in blackwater soils was lower when 400 kg N ha^–1, but not when 200 kg N ha^–1, was added. The active fungal biomass in blackwater soils was higher when 400 kg N ha^–1, but not when 200 kg N ha^–1, was added. The active fungal biomass in redwater wetland soils was lower when 200 kg N ha^–1, but not when 400 kg N ha^–1, was added. Cellulose and lignin degradation was higher in redwater than in blackwater soils. After 10 and 15 weeks of incubation, the addition of 200 or 400 kg N as NH₄NO₃ ha^–1 decreased cellulose and lignin degradation in both wetland soils to similar levels. This study indicated that the addition of N may slow organic matter degradation and nutrient mineralization, thereby creating deficiencies of other plant-essential nutrients in wetland forest soils. Received: 7 April 1999     

Influence of nitrogen on atrazine and 2, 4 dichlorophenoxyacetic acid mineralization in blackwater and redwater forested wetland soils

 Microcosms were used to determine the influence of N additions on active bacterial and fungal biomass, atrazine and dichlorophenoxyacetic acid (2,4-D) mineralization at 5, 10 and 15 weeks in soils from blackwater and redwater wetland forest ecosystems in the northern Florida Panhandle. Active bacterial and fungal biomass was determined by staining techniques combined with direct microscopy. Atrazine and 2,4-D mineralization were measured radiometrically. Treatments were: soil type, (blackwater or redwater forested wetland soils) and N additions (soils amended with the equivalent of 0, 200 or 400 kg N ha^–1 as NH₄NO₃). Redwater soils contained higher concentrations of C, total N, P, K, Ca, Mn, Fe, B and Zn than blackwater soils. After N addition and 15 weeks of incubation, active bacterial biomass in redwater soils was lower when N was added. Active bacterial biomass in blackwater soils was lower when 400 kg N ha^–1, but not when 200 kg N ha^–1, was added. Active fungal biomass in blackwater soils was higher when 400 kg N ha^–1, but not when 200 kg N ha^–1, was added. Active fungal biomass in redwater soils was lower when 200 kg N ha^–1, but not when 400 kg N ha^–1, was added. After 15 weeks of incubation 2,4-D degradation was higher in redwater wetland soils than in blackwater soils. After 10 and 15 weeks of incubation the addition of 200 or 400 kg N ha^–1 decreased both atrazine and 2,4-D degradation in redwater soils. The addition of 400 kg N ha^–1 decreased 2,4-D degradation but not atrazine degradation in blackwater soils after 10 and 15 weeks of incubation. High concentrations of N in surface runoff and groundwater resulting from agricultural operations may have resulted in the accumulation of N in many wetland soils. Large amounts of N accumulating in wetlands may decrease mineralization of toxic agricultural pesticides. Received: 26 June 1998     

Poultry litter as a nitrogen and phosphorous source for the rice–wheat cropping system

Poultry litter (PL) is an important nutrient source; however, no information is available regarding its value in supplying N and P in rice–wheat (RW) production. A three-year field study was conducted at Ludhiana, Punjab, India on a loamy sand soil to identify optimum combination of PL and N and P fertilizers for a sustainable RW production. The litter was applied to rice at 5 Mg ha⁻¹ as a single application and supplemented with different rates of N. The residual effect of PL and the direct effects of the different combinations of N and P were studied in the following wheat. Nitrogen and P mineralization from PL was studied under controlled conditions in the laboratory, and macronutrient input–output balances were estimated from field results. About 46% of the N from PL was released after 60 days of incubation. The release of P from the PL occurred mainly during the initial 20 days after incubation, accounting for 15–17% of the total P. Combining PL with fertilizer N (40 kg ha⁻¹) increased rice yield and nutrient uptake similar to what was obtained with the application of recommended fertilizer N (120 kg ha⁻¹). In the following wheat, the residual effect of PL was equal to 30 kg N ha⁻¹ and 13 kg P ha⁻¹. After three annual cropping cycles and PL application, mean soil organic C increased by 17%, Olsen-P by 73%, and NH₄OAc-extractable-K by 24%. Most treatments had positive P but negative K balances. About 11% of the net P balance was recovered from the soil as Olsen-P. The study showed that optimum N and P fertilizer doses for an RW system receiving 5 Mg ha⁻¹ of PL are 40 kg N ha⁻¹ for rice and 90 kg N + 13 kg P ha⁻¹ for the following wheat. Safe and effective management of PL should be based on P balance, particularly when regular applications of PL are to be made in the RW system.     

Organic residues affect phosphorus availability and maize yields in a Nitisol of western Kenya

 The effects of organic residues and inorganic fertilizers on P availability and maize yield were compared in a Nitisol of western Kenya. Leaf biomass of Calliandra calothyrsus, Senna spectabilis, Croton megalocarpus, Lantana camara, Sesbania sesban, and Tithonia diversifolia were incorporated into the soil at 5 Mg ha^–1 for six consecutive seasons in 3 years and responses compared with those following the application of 120 kg N ha^–1, 0 kg P ha^–1 (0P); 120 kg N ha^–1, 10 kg P ha^–1; and 120 kg N ha^–1 25 kg P ha^–1 as urea and triple superphosphate (TSP); K was supplied in all treatments. Addition of Tithonia, Lantana and Croton increased soil resin-extractable P over that of fertilizer-amended soil throughout the first crop, but the amounts in the former treatments became similar to those for soils amended with inorganic fertilizers for subsequent crops. Addition of Sesbania, Calliandra and Senna had a similar effect on resin P as inorganic fertilizers. Total maize yields after six seasons were tripled by the application of Tithonia compared to 0P, and were higher than those of the Calliandra, Senna, Sesbania and Lantana treatments, and similar only to that of the Croton treatment. P recovered in the above-ground biomass and resin P, immediately after the implementation of the treatments, was higher in the Senna, Sesbania, Croton, Lantana and Tithonia (35–77%) treatments than in the inorganic fertilizer treatments (21–27%). The P content of organic residues, and the soluble C:total P ratio, were the main residue parameters predicting soil P availability and maize yield. All organic residues used in this study can replace inorganic fertilizers for the enhancement of P availability and maize production, while an additional benefit could be obtained from the use of Croton, Lantana and Tithonia. Received: 19 January 2000     

Evaluating N2 fixation by food grain legumes in farmers’ fields in three agro-ecological zones of Zambia, using 15N natural abundance

A survey of N₂ fixation in farmers’ fields of Northern (>1,000 mm rainfall), Central (800–1,000 mm rainfall), and Southern (<800 mm rainfall) Zambia revealed some significant differences in plant growth and symbiotic performance of different food grain legumes. Of the three grain legumes (i.e., Bambara groundnut (Vigna subterranea L. Verdc.), cowpea (Vigna unguiculata L. Walp.), and groundnut (Arachis hypogaea L.)) grown in Southern Zambia, cowpea showed greater shoot biomass and significantly lower shoot δ¹⁵N values than groundnut and Bambara groundnut. The lower shoot δ¹⁵N resulted in greater %Ndfa (59%) in shoots and higher amounts of N-fixed, whether per square meters (6,394.0 mg N), per plant (650.8 mg N), or per hectare (63.9 kg N) relative to groundnut and Bambara groundnut, even though the number of cowpea plants per square meter was significantly lower than that of groundnut or Bambara groundnut. Although the shoot δ¹⁵N values of cowpea, Bambara groundnut and common bean (Phaseolus vulgaris L.) were significantly lower than those of groundnut in Central Zambia and their %Ndfa values, therefore, greater, the higher number of groundnut plants per square meter resulted in significantly greater shoot N content, as well as N-fixed per square meter and per hectare relative to the other species. Despite having similar plant density as cowpea in Central Zambia, common bean could fix only 6.0 kg N ha⁻¹ compared with 35.4 kg N ha⁻¹ by cowpea. In Northern Zambia, Bambara groundnut showed the lowest mean shoot δ¹⁵N value (0.54 ± 0.3‰), followed by groundnut (1.59 ± 1.0‰), and then common bean (the three grain legumes grown in that region). As a result, %Ndfa and N-fixed were significantly greater in groundnut (69.7% and 566.0 mg N per plant) and Bambara groundnut (62.9% and 440.1 mg N per plant) than in common bean (2.6% and 2.4 mg N per plant). In Northern Zambia, groundnut, Bambara groundnut and common bean fixed 78.7, 67.6, and 0.9 kg N ha⁻¹, respectively, even though the plant density per square meter of common bean (which fixed the lowest amount of N per hectare) was twice that of groundnut and Bambara groundnut. A species × site analysis showed that cowpea fixed relatively greater amounts of N per plant, per square meter, and per hectare in Southern than Central Zambia. Bambara groundnut and common bean also had significantly lower δ¹⁵N values and higher %Ndfa in Central than Northern Zambia.     

Effectiveness of native Rhizobium on nodulation and yield of faba bean (Vicia faba L.) in Eastern Ethiopia

This study was initiated to evaluate the effect of locally isolated Rhizobium on nodulation and yield of faba bean at Haramaya, Ethiopia for three consecutive years. Ten treatments comprising of eight effective isolates of rhizobia, uninoculated, and N-fertilized (20 kg N ha^?1) were laid out in a randomized complete block design with three replications. The result of the experiment indicated that all inoculation treatments increased nodule number and dry weight over the control check in all cropping seasons. The result, however, showed the non-significant effect of Rhizobium inoculation on shoot length, number of tiller per plant and 100 seed weight in all cropping season. Inoculating Haramaya University Faba Bean Rhizobium (HUFBR)-15 in 2011 and National Soil Faba Bean Rhizobium (NSFBR)-30 in 2012 and 2013 gave the highest grain yields (4330, 5267 and 4608 kg ha^?1), respectively. These records were 75%, 48%, and 5% over the uninoculated treatment of respective years. Over the season, NSCBR-30 inoculation resulted in the highest nodulation and grain yield production as compared to the other treatments. In general, isolates from central Ethiopia were better than those isolated from eastern Ethiopia and Tropical Agricultural Legume (TAL)-1035 in enhancing faba bean production at Haramaya site. Therefore, NSFBR-30 is recommended as a candidate isolate for faba bean biofertilizer production in eastern Ethiopia soils.