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芦荟吸芽离体培养和植株再生 总被引:1,自引:0,他引:1
芦荟吸芽在MS+BA3-4mg/L+NAA 0.5-0.7mg/L的培养基中,产生丛生芽;转入MS+BA 2.0-3 NAA0.5-0.7mg/L继代培养时,芽生长健壮;切割不定芽在1/2MS+ABT1.5-2.0mg/L培养基中可诱导生根,生根率达90%以上。每年3-6月,9-10月移栽于河沙:Luo石为3:1的基质内,移栽成活率达80%以上。 相似文献
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冀北山区日本落叶松半木质化插穗夏插30天后开始生根,从扦插至大部分插穗生根需要62天。插穗经200ppmIBA溶液处理20min后,生根率可达76^,延长处理时间至40min,生根率可提高到86%以上。日本落叶松半同胞有系,母株间插穗生根率变异幅度大,分别为40%=92%,14.2%-100%。生根性状在二种水平上均存在有巨大的改良潜力。因此,日本落叶松扦插繁殖生要物改良要实行家系选择和母株选择兼 相似文献
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采用文中所介绍的技术、龙眼扦插40天后即产生愈伤组织,愈合率达86.6%;60-90天大量生根,生根率达87.2%成活率达74.5%;90天后即可进行苗地移栽,成活率达90.5%,1年一苗高60-150cm,地径0.6-1.5cm,此时即可邮圃定植,2-3年挂果,4年进入丰产期,丰产效果显著。 相似文献
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毛果含笑扦插繁殖法研究 总被引:4,自引:0,他引:4
本文报道了扦插基质、NAA浓度、光照强度、插条的光合面积对毛果含笑(MicheliasphaeranthaC.y.WuetLaw)插条生根的影响.结果表明,扦插基质以珍珠岩:腐叶土:红壤=1:1:1混合最好,生根率达62%;NAA150×10-6处理校好,特别是当愈伤组织形成后,取出插条再次用150×10-6的NAA浸泡4小时效果更佳,生根率达72%且可使插条比处理一次的提前20~30天生根;搞条带两片叶子生根较好,生根率达68%,并且根系祖壮发达,在60%的透光下生根较好. 相似文献
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赤桉试管苗移栽及插插技术研究初报 总被引:2,自引:2,他引:0
组培苗自组培室移出3d内栽完的成活率为74%,11d内栽完的降低至28.7%;扦插时气温15.6℃以上生根率可达到89.4%至98%,12℃以下生根率只有71.3%;2号ABT生根粉100mg/L液浆处理插穗,生根率为95.5%, 相似文献
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Developing methods for routine clonal propagation of sandalwood (Santalum austrocaledonicum) is important for its domestication and development as a commercial agroforestry species. The amenability of this species to propagation by leafy stem cuttings in low-cost non-mist propagators was assessed in four separate experiments. These experiments evaluated the effects of (1) genotype (15 genotypes from two island provenances), (2) cutting position on the stock plant (apical, medial and basal), (3) cutting size (1-node/400 mm2 and 2-node/800 mm2 leaf area), (4) three propagation media [scoria (5 mm, air-filled porosity (AFP)—29%), vermiculite and perlite (1:1 v/v, AFP—46%) and vermiculite, perlite and peat (2:2:1 v/v/v, AFP—42%)], (5) indole-3-butyric (IBA) (3000, 4000 and 8000 ppm) and (6) irradiance in the propagator [daily light integral (DLI) 5.3, 3.9, 2.6, 2.2 mol m?2 day?1]. IBA, propagation media and cutting size had no significant effect on rooting percentage, root number or root growth. Evidence of provenance-based variation in rooting capacity was recorded with greater rooting success for genotypes from the island of Erromango compared with Tanna. Variation in adventitious root induction was also recorded between individual genotypes from Erromango across all four experiments. Cuttings collected from the apical and medial parts of the shoot on the stock plant had higher rooting percentage than those collected from the base. Differences in rooting capacity between apical and medial shoots were variable between experiments and may be attributed to different levels of hardening. The use of artificial shade (~?70%) to achieve a mean DLI of between 2.6 and 3.9 mol m?2 day?1 increased rooting percentage compared to both lower (2.2 mol m?2 day?1 or?~?90% shade) and higher (5.3 mol m?2 day?1 or?~?50% shade) irradiance treatments. Leaf retention of cuttings in the propagator was positively associated with the percentage of cuttings with adventitious roots, with highest percentage in cuttings with full leaf retention, regardless of original cutting size. This study demonstrated S. austrocaledonicum seedlings can be successfully propagated by cuttings provided the propagation conditions are optimized for each genotype. 相似文献
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Catherine Ky-Dembele Jules Bayala Antoine Kalinganire Fatoumata Tata Traoré Bréhima Koné Alain Olivier 《Southern Forests》2016,78(3):193-199
Pterocarpus santalinoides is a multipurpose tree species, native to Africa and South America. However, excessive pruning for fodder is jeopardising its seed production leading to poor regeneration in the Sahel region. Efficient vegetative propagation techniques may overcome this concern. To address this issue, four experiments were conducted to determine the effects of substrate (Experiment 1), cutting type (Experiments 2 and 3), genotype (Experiment 4) and auxins (Experiments 1, 2, and 4) on rooting ability in a non-mist propagating unit in Mali. Among the four tested substrates, no significant differences were found in rooting parameters, including the percentage of rooted cuttings, the number of roots and secondary roots per rooted cutting, and the length of the longest root per rooted cutting. Semi-hardwood cuttings rooted significantly better than softwood cuttings with a higher percentage of rooted cuttings (31.7?±?6.5% vs. 9.2?±?2.7%), greater number of roots (8.2?±?4.8 vs 1.5?±?0.7), greater number of secondary roots (16.7?±?4.2 vs 4.6?±?1.8) and longer root length (17.8?±?12.0?cm vs 2.3?±?1.0?cm) per rooted cutting. In addition, one-node cuttings cut below the second and third nodes, respectively, had the highest rooting success (88% and 90%, respectively) followed by cuttings cut below the fourth node (68%) and the first node (62%). Significant differences in the percentage of rooted cuttings were found among 12 randomly selected genotypes with rooting varying from 2% to 75%.The results also showed that auxin application, especially IBA, had a significant effect on rooting success, the number of roots and secondary roots per rooted cutting. Single-node cuttings, and cuttings of 10?cm length with multiple softwood and semi-hardwood nodes, can be used with or without auxin treatment for successful clonal propagation of P. santalinoides. The results of the present study indicated that P. santalinoides is amenable to clonal propagation using low-cost propagators and sand-based rooting media. 相似文献
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落叶松扦插繁殖配套技术的研究 总被引:1,自引:0,他引:1
从3个主要途径收集了落叶松优良品系,建立了采穗圃,营造了对比试验林。对优良品系无性苗扦插技术进行了较全面研究,结果表明:(1)在扦插基质选择上,以河沙为好;(2)插穗地径与生根有较大关系,采穗时应尽量剪取地径大于1.8 mm的插穗;(3)母树年龄越小,插穗生根效果越好,据此,采穗圃以经营5 a为宜;(4)不同品系落叶松的扦插生根能力不同;(5)不同配比、不同浓度的激素至关重要,采用IBA50%+IAA20%+NAA30%的质量分数为5.0×10-5 浓度溶液和IBA60%+IAA20%+NAA20%的质量分数为1.5×10-4 浓度溶液,辅以适量烟酸及链霉素等增效剂处理落叶松插穗,其生根可稳定在80%以上。 相似文献
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[目的]为欧洲云杉扦插穗条选择和扦插苗培育提供依据。[方法]采用完全随机区组试验设计,研究了2种穗条类型(嫩枝基部带1.5年生硬枝、嫩枝)、3种穗条长度(5 10、10 15、15 20 cm)的嫩枝扦插苗从移植当年到第3年的成活率、保存率和生长情况。[结果]2种穗条类型扦插苗间的移植成活率和保存率差异不显著,但移植后嫩枝基部带1.5年生硬枝扦插苗的苗高、新梢生长量、地径、侧枝数、冠幅、鲜质量、干质量差异显著,都是嫩枝基部带1.5年生硬枝扦插苗大于嫩枝扦插苗。3种长度扦插苗的移植成活率、保存率的差异均显著;其中,15 20 cm长度穗条扦插苗移植成活率和保存率最高,分别是98.00%和86.00%,不同长度穗条扦插苗移植成苗率逐年下降,而且与穗条长度有关,弱小穗条获得的扦插苗成苗率低;5 10 cm的扦插苗移植成活率为46.00%,到第3年时保存率仅为23.33%,且移植后苗高、新梢生长量、地径、侧枝数、冠幅、鲜质量、干质量的差异也显著,且都以15 20cm穗条的扦插苗值最高,10 15 cm穗条的扦插苗次之,5 10 cm的扦插苗最小。嫩枝带硬枝扦插苗的苗木质量指数(QI)是嫩枝的7.74倍,15 20 cm穗条的扦插苗质量指数(QI)是5 10、10 15 cm扦插苗的5.47、5.23倍。扦插苗地径初始值与移植当年地径、苗高初始值与移植当年苗高间呈极显著正相关关系。[结论]扦插苗最初的生根状况和穗条质量影响后期培育过程中的存活和生长,嫩枝基部带1.5年生硬枝扦插苗和15 20 cm穗条的扦插苗不仅移植成活率、保存率高,而且生长量、生物量、质量指数都比短而弱小穗条的扦插苗高。 相似文献
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在无激素处理的条件下,1.5年生幼龄母株的插穗易于生根,平均生根率达96.0%以上,且发根最大,偏根率低;4.5年生时,插穗生根率极显著低于1.5年生母株,而与8.5年生母株无显著差异;12.5年生母株插穗生根率又极显著低于8.5年生母株。母株年龄与插穗生根率和偏根率分别呈显著的负相关(r=-0.9652)和极显著正相关(r=0.9993),但与生根量和平均最长根长度无显著相关。插穗基部切削方式不 相似文献
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Poplar shoots trained horizontally and normally growing shootswere used to compare the effect of horizontal and vertical orientationin summer on the subsequent rooting of terminal cuttings; asignificantly greater number of preformed roots emerged fromcuttings of vertical shoots. This difference in rooting abilitymay be due to the lower concentration of indolyl-acetic acidin the upper than in the lower half of horizontal poplar stemsas shown by Leach and Wareing (1967). Decapitation of a seriesof these poplar cuttings (Populus ? robusta Schn.) showed thatwound-root production was influenced by the larger terminalbuds on vertical than on horizontal shoots. From a comparison of horizontal and vertical storage of applerootstock cuttings at 45 ?F in the autumn, 29 per cent moreof the horizontal cuttings than the vertical ones had a basalcallus in the following February. Of these cuttings which madesatisfactory shoot growth (over 5 cm) in the following summer,horizontal cuttings exceeded vertical ones by 80 per cent; aresult which was correlated with root production. In a second experiment with M II apple rootstocks in which cuttingswere both stored and grown at the same angle more roots occurredon cuttings placed horizontally rather than vertically, andthis orientation also induced the rootings of a greater proportionof leafy Worcester Pearmain apple cuttings. 相似文献
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沙松扦插繁殖技术研究 总被引:1,自引:0,他引:1
以沙松硬枝和嫩枝插穗为材料进行正交扦插试验,研究穗条母株年龄大小、药品种类、质量浓度、处理时间以及基质不同种类、穗条不同类型与不同着生部位对生根的影响,结果表明:影响硬枝和嫩枝扦插生根的主要因素均为穗条母树年龄大小,年龄越小,生根率越高,最优处理组合均为4~10 a母树穗条,用200 mg·L-1ABT1号生根粉浸泡12 h,生根率分别为21.4%、63.9%;嫩枝扦插生根率高于硬枝扦插,差异达到极显著水平。采用1/2珍珠岩+1/2河沙混合基质生根效果最好。穗条不同类型扦插生根存在差异,一级侧枝截制的穗条生根性状优于二级侧枝穗条和顶芽穗条,下部枝条截制的插穗生根率和根系效果均高于上部枝条。 相似文献