POCT技术在母猪早期妊娠诊断中的应用

<正>母猪的早期妊娠诊断是提高母猪繁殖效率和畜牧业生产效益的重要技术措施,尤其是配种后20天前后的早期妊娠诊断是母猪繁殖技术中十分重要的内容。通过诊断,可以尽早地确定母猪是否妊娠,以     

母猪早期妊娠常用诊断技术及新技术研究进展

<正>母猪的早期妊娠诊断是提高母猪繁殖效率和畜牧业生产效益的重要技术措施,尤其是配种后20d前后的早期妊娠诊断是母猪繁殖技术中十分重要的内容。通过诊断,可以尽早的确定为妊娠母猪,以便及时采取措施,     

母猪早期妊娠诊断技术的研究进展

<正>随着集约化养猪业的发展,母猪早期妊娠诊断对提高母猪繁殖率和养猪场的经济效益方面的作用越来越明显。对母猪妊娠做出及时而准确的判断,可以减少空怀,并对空怀的母猪进行补配,从而减少因无效饲养增加的饲料成本。本文就母猪早期妊娠诊断技术作一概述。1超声波诊断     

猪场B超早期妊娠诊断声像图识别研究

为了在养殖过程中更好地利用B超对母猪进行早期妊娠诊断,试验采用便携式兽用B超仪对300头次母猪进行早期妊娠诊断;同时对11头母猪进行了跟踪检测,得到各时期的声像图及相应的诊断数据。结果表明:B超早期妊娠诊断准确率在配种后21天即可达到100%。     

母猪早孕胶体金检测试纸法与其它方法的比较

母猪的早期妊娠诊断是提高母猪繁殖效率和养猪业生产效益的重要技术措施,母猪配种后,能够尽早地妊娠确诊,能够减少母猪的空怀期或非繁殖天数,可以提高母猪的平均年产仔窝数,并有利于对繁殖障碍母猪的及早治疗处理。对比目前常见的母猪早期妊娠诊断方法:返情观察法、注射雌激素检测法、直肠触诊法、超声波诊断法、血小板计数法、激素测定法及激素测定法新的应用——免疫胶体金快速检测法,对比各方法优劣,试图找出适合广大养猪户的具有实用性的母猪早期妊娠诊断方法。     

兽用B超在母猪早期妊娠诊断中的应用思考

介绍了兽用B超对母猪早期妊娠诊断应用的原理、方法与标准。与传统诊断技术相比,兽用B超探查具有明显优势,母猪的妊娠诊断完全可在25～39 d进行,对早期妊娠诊断意义重大。通过对武汉市安渡种猪场最近4年的试验数据分析,表明兽用B超母猪早期妊娠诊断可以大幅度地减少规模猪场无效饲养,提高猪群发情期受胎率,增加猪场经济效益。     

母猪早期妊娠诊断技术应用概况

本文就母猪各种早期妊娠诊断方法的原理及应用现状进行了概述。并认为，早期妊娠诊断技术是解决母猪空怀、缩短产仔间隔的根本途径。     

血小板计数法对母猪早期妊娠的诊断研究

应用血小板计数法对母猪早期妊娠进行诊断,结果表明:10头未孕母猪的血小板测定值为323.9×109/L±3.9×109/L;10头妊娠母猪的血小板测定值在妊娠第7天减少到251.9×109/L±8.6×109/L(P＜0.01).根据妊娠母猪配种前后血小板值之差≥72×109/L为妊娠标准,对20头受配母猪进行了早期妊娠诊断,经与超声波诊断结果比较,妊娠母猪符合率为87.5%(P＞0.05).     

浅谈母猪早期妊娠的诊断方法

妊娠诊断是母猪繁殖管理上的一项重要内容。配种后，应尽早检出空怀母猪，及时补配，防止空怀。这对于保胎、缩短胎次间隔、提高繁殖力和经济效益具有重要意义。母猪早期妊娠诊断技术方面的研究很多，笔者在此将近年来较成熟、简便，并具有实际应用价值的早期妊娠诊断技术综述如下。     

B型超声波技术在母猪早期妊娠诊断上的应用研究

<正>妊娠诊断技术是减少猪场非生产天数、提高母猪繁殖效率的一项重要手段。常见的母猪早期妊娠诊断方法有公猪试情、直肠检查、阴道检查、激素反应、尿液检查、血小板计数等〔1〕,但这些方法往往存在误差大、操作繁琐、应激大等缺点。近十年来,代替以上诊断方法的诸多类型妊娠诊断仪器相继问世,具有安全省时、操作简便、准确率高的B型超声波测定技术越来越多地应用到母猪早期妊娠诊断中。但至今仍缺乏有关使用B超进行母猪早期     

Pregnancy diagnosis in sows: a comparison of the vaginal biopsy and Doppler ultrasound techniques.

Two pregnancy diagnosis techniques, vaginal biopsy and ultrasonic detection, were applied to 190 sows following service. Of 172 sows diagnosed pregnant by vaginal biopsy, five did not farrow. Of 18 sows judged not pregnant, one produced a litter. With the ultrasound technique, 175 sows were diagnosed pregnant, but 11 did not farrow. Of the remaining 15 sows considered not pregnant by the 39th day after service, four farrowed. The biopsy result was usually available up to 48 hours earlier than the ultrasound result. It was concluded that where laboratory facilities are available, the biopsy technique is the method of choice.     

Reliability of pregnancy diagnosis in sows using direct radioimmunoassay of estrone-sulfate

The pregnancy diagnosis in sows using direct radioimmunoassay of estrone-sulphate in the blood serum without sample extraction is described. It was found that for the pregnancy diagnosis the period between days 22 and 30 of pregnancy can be used since in this period the estrone-sulphate concentrations in all pregnant sows markedly exceeded those of 64 non-pregnant animals. The estrone-sulphate estimation cannot be used for the pregnancy diagnosis before day 22 and between day 30 and 40 because the estrone-sulphate concentrations in most samples collected in these periods were lower than 4 nmol.l-1.     

Glomerular capillary endotheliosis in sow kidney

A high incidence of proliferative renal glomerular lesions was found in sows from a breeding herd. All the sows had a very similar lesion, which was characterised by swelling of endothelial cells, with varying degrees of obstruction of the capillary lumina, and an increase in the number of mesangial cells. By differential diagnosis the condition was found to conform most closely with the renal lesion of human pregnancy toxaemia (pre-eclampsia syndrome). It is suggested that this nephropathy may play a part in limiting the useful breeding life of affected sows.     

Pregnancy diagnosis in Swine: a comparison of two ultrasound instruments

This investigation was designed to assess the sensitivity, specificity and positive predictive values of amplitude-depth and Doppler pregnancy detectors when utilized for pregnancy diagnosis of mated sows. Mated sows (n = 38) were tested daily from 15 to 45 days after mating for pregnancy with the ultrasound instruments. The same procedure was performed with nonmated sows (n = 10) 15 to 45 days after estrus. Both instruments were unreliable for pregnancy diagnosis between 15 and 22 days after mating. Between 23 and 45 days after mating the Doppler pregnancy detector was more specific and had greater positive predictive values. In contrast the amplitude-depth pregnancy detector was more sensitive during the same time interval.

Serum estrone sulphate concentrations were determined in samples collected between 27 and 30 days after mating or estrus, as an alternate method of pregnancy diagnosis. Serum estrone sulphate concentrations were always equal to or greater than 0.5 ng/mL in the pregnant sows, while in the nonmated sows estrone sulphate concentrations were never more than 0.5 ng/mL serum.

     

Accuracy of Trans-abdominal Ultrasound Pregnancy Diagnosis in Sows using a Linear or Sector Probe

The assessment of an early and reliable pregnancy diagnosis in sows is very important for limiting the number of non-productive days in sow herds. The aim of the present study was to investigate the accuracy of trans-abdominal ultrasound detection for early pregnancy diagnosis in sows under field conditions. Particular attention was paid to the time when a reliable diagnosis can be made, and to possible differences between two commonly used types of scanners. In total, 202 sows from two herds were tested daily with scanners A (linear transducer of 5 MHz) and B (sector transducer of 3.5 MHz) from day 16 until 25 after insemination. Ninety-three percentage of the sows were pregnant 36 days after insemination. Using ultrasound, the first pregnancy could be detected already 18 days after insemination. The sensitivity, specificity and overall accuracy were higher than 95% from day 24 (scanner A) or day 23 (scanner B) post-insemination onwards. The positive predictive values from day 19 onwards were consistently higher than 95% with both types of scanners, whereas the negative predictive values were consistently low for both scanners during the entire period. The sensitivity of testing tended to be slightly lower in sows with a litter size of <10 total born piglets, compared to sows with 10 or more piglets. In conclusion, pregnancy diagnosis in sows using trans-abdominal ultrasound testing can be assessed quickly and reliably under field conditions from day 23 of gestation onwards. Predictive values of positive test results were high in these herds, whereas those of negative test results were low. This implies that sows with a negative test result early in pregnancy should be retested later.     

Eradication of Salmonella Yoruba in an integrated pig herd

An integrated SPF herd with 320 sows was found infected with Salmonella Yoruba during an annual control among sows, aiming to verify freedom from Salmonella infections. It is believed that the infection was introduced to the herd by purchase of feed. The herd performed an age segregated rearing system. Sows and piglets were reared at a central farm, while growers (25-100 kg body weight) were reared at sub-estates. The growers were free from the infection, and as a consequence a specially designed eradication program was designed. Farrowing and weaning were defined as periods of risk for sows and piglets, respectively. Consequently sows were isolated and individually tested for presence of Salmonella one week before and one week after farrowing. The offspring were tested one week post weaning. To verify freedom from disease among piglets they were also tested another time before transfer to the uninfected sub-estates. Piglets with undefined status regarding Salmonella were denoted animals at risk and not transferred to the sub-estates. Instead they were transferred to a third estate, rented to house pigs at risk. The program was successful. It allowed full production during performance, and the herd was declared free from S. Yoruba seven and a half months after the initial diagnosis.     

母猪妊娠早期血液中差异表达蛋白的鉴定

为了研究母猪妊娠早期外周血液中蛋白质的表达情况,寻找潜在的母猪妊娠早期生物标志物,本试验采集了 8头健康、生产表现正常且体况相近的配种后15 d妊娠与非妊娠的长大二元经产母猪外周血液,分离蛋白,利用iTRAQ技术及生物信息学方法筛选与胚胎附植相关的差异表达蛋白,用ELISA验证其表达情况,并进行ROC曲线分析.结果显示...     

便携式B超在母猪早期妊娠诊断中的应用研究简

The pregnancy rates at 20 and 50 days were tested by sampling 91 hybridization sows more than 16 days using the intuitive, fast, accurate and non-invasive portable veterinary B ultrasound instrument in the pig breeding farm had 300 sows. The results showed that the nonpregnant diagnostic accuracy rate was 100% at 18 to 20 days, and the pregnant diagnostic accuracy rate was 100% at 40 to 50 days. According the diagnostic results, the pregnancy, disease, estrus, nonpregnancy and non-estrused sows were took the appropriate measures such as nursing, treatment, supplementary breeding and sensuality, the non-productive days of the individuals and groups of sows were improved by above measures. The study built a intuitive, fast, accurate sows early pregnancy diagnosis technology, which improved the fertility of the individuals and groups of sows increased the economic benefit of pig farm.     

新型激素制剂对母猪诱导发情效果研究

应用PX-700制剂对乏情母猪进行了诱导发情研究，结果表明：700IU是PX-700制剂诱导乏情母猪发情的适宜剂量；而该制剂对断奶后45d以上的乏情母猪诱导发情率较低，但其情期受胎率不受影响；PX-700制剂对诱导发情后母猪的情期受胎率、产仔数及仔猪出生重均无影响。     

Microbial changes as the cause of failures of the therapy of bacterial diseases of the urogenital organs of swine

In long-term clinical and bacteriological controlled breeding sows of 15 stocks with a high rate of urinary tract infections in 41 sows (= 8.97%) of 457 chronic infected sows a spontaneous change of the causative microorganism was seen. In clinical trials to test the effectiveness of new chemotherapeutics in sows suffering from urogenital infections changes of bacteria were noticed during and after therapy. In selected cases the proceeding of change using a quinoxaline-di-N-oxide-derivative and an aminoglycoside-antibiotic is demonstrated. After parenteral application of the quinoxaline-di-N-oxide-derivative in 3 of 6 cases a change of the causative agent happened. Treating the sows with the aminoglycoside-antibiotic there was a change in 18.2% of urinary tract infections and in 25.0% of genital tract infections. The causes of changes of the causative microorganisms are discussed and conclusions are drawn for diagnosis and therapy of urinary and genital tract infections in the sow.