Effect of plant density on stolon growth and development of contrasting white clover (Trifolium repens) varieties and its influence on the components of seed yield

Plants of two contrasting white clover varieties (cv. Aberystwyth S184 and Olwen) were planted in the field in spring in each of 2 years at four densities (9, 25, 49 and 100 plants m⁻²) in 1-m² plots. The effect of plant density on stolon growth and development and the components of seed yield was subsequently measured.
Stolon growth and development was influenced by plant density, variety and year. At low plant densities both white clover varieties produced longer primary stolons than at higher densities. Plant density, however, had no significant effect on the number of inflorescences at harvest. At the high stolon densities there were significantly fewer reproductive nodes per primary stolon than at the lower plant densities. Plant density did not significantly affect any other seed yield components, but the number of inflorescences at harvest, florets per inflorescence, seed set per floret and 1000-seed weight were all significantly influenced by both variety and year.
The relationship between the vegetative and reproductive growth of white clover is discussed in relation to plant density, variety and climate and the possible role of defoliation managements on inflorescence development.     

Evaluation of seed production potential in white clover (Trifolium repens L. varietal improvement programmes

The components of actual and potential seed yield were examined in field experiments on a wide range of varieties of white clover (Trifolium repens L.). The factors affecting seed yield under conditions typical of management regimes used in the production of commercial seed crops were assessed in two experiments. In the first, carried out on spaced plants, considerable diferences are shown between six varieties across the range of leaf sizes in the distribution and profuseness of inflorescence production through the flowering season. In the second, carried out in plots, typical on-farm criteria were used to choose a single harvest date. Significant variation was found between varieties, including representatives of different leaf size categories, for seed yield components that include number of inflorescences m^?2, number of florets per inflorescence and harvestable seed weight. Large-leaved varieties tended to produce more seeds per floret and higher seed weights per inflorescence, whereas small-leaved varieties gave the highest number of inflorescences ^?2. However, the small-leaved variety AderDale, selected for strong peduncles, was exceptional, giving high values for all seed yield components. The impact of weather conditions on many seed yield components (e.g. total number of inflorescences) was demonstrated by the differences between the 2 years of the experiment. However, other characterstics, e.g. number of florets per inflorescence and number of seeds per floret, did not vary between years. Deviations from potential seed yield were assessed fromthe perspective of commerical seed production. The implications of these results for the production of white clover varieties with increased seed yields under UK conditions are discussed.     

The effect of agronomic factors on seed and forage production in perennial legumes sainfoin (Onobrychis viciifolia Scop.) and French honeysuckle (Hedysarum coronarium L.)

Perennial legume varieties and ecotypes belonging to the species sainfoin ( Onobrychis viciifolia Scop.) and French honeysuckle ( Hedysarum coronarium L.) were investigated under different management treatments (two regimes of irrigation, cutting and seeding densities) at the Forage Crops Institute in Foggia, Italy (which has a typical Mediterranean environment) for two seasons, 1990 and 1991. Dry-matter production, seed production and seed yield components were affected more by harvest year and irrigation than by seeding density. Mean increases due to irrigation were 2·8% and 60·6% respectively for dry-matter and seed yield in sainfoin and 35·3% and 32·5% respectively in French honeysuckle. Under both cutting regimes, the higher seeding density was more suitable for sainfoin, increasing stems m⁻² with irrigation and seed yield without irrigation. In French honeysuckle the lower seeding density was more suitable for production of inflorescences per tiller and for seed weight without irrigation. Higher seeding density positively influenced seed yield under irrigation. The seed yield components most influenced by irrigation were: 1000-seed weight, seeds per inflorescence and inflorescence per stem. French honeysuckle genotypes were potentially more productive in dry matter and seed yield under both irrigation regimes than the sainfoin, which was more productive in seed yield when cut. The ecotypes of both legumes represent a genetic resource, particularly for seed yield after forage cutting, to be utilized in breeding programmes for developing varieties well adapted to Mediterranean environments.     

The effect of time of harvest on seed production of three red clover cultivars

Small plots of red clover cv. Sabtoron, S123 (diploids) and Hungaropoly (tetraploid) were harvested for seed production at two-week intervals from 19 August to 17 October inclusive in 1981, inflorescence appearance rate, bee density and components of yield having been monitored throughout the summer. Inflorescence appearance rate reached a maximum at the end of July/early August for Hungaropoly and Sabtoron and during mid-August for S123. Bee density followed a similar pattern. Florets and seeds per inflorescence and 1000-seed weight decreased as flowering progressed except during the first three weeks in July. Potential seed yield was calculated from the components of yield for the harvests taken on 3 and 18 September. Losses in seed yield (difference between actual and potential) up to and during harvesting, threshing and cleaning were lower in the tetraploid cultivar (27–39%) than the diploid cultivars (35–l55%). Each cultivar had an optimum harvest time before which yield was affected by immature seeds and beyond which it was adversely affected by shedding of inflorescences and seeds and sprouting of seeds on the inflorescences. The optimum time to harvest Sabtoron was early September, Hungaropoly early to mid-September and the late flowering cultivar S123 mid-September when less than 4% of the inflorescences were still unripe. Seed yield and inflorescences per unit area were lower in the tetraploid cv, Hungaropoly (maximum 542 kg ha⁻¹) than diploid cv. Sabtoron and S123 (864 and 897 kg ha⁻¹ respectively) although the tetraptoid had heavier seeds. It is concluded that the optimum time to harvest red clover for seed production is about three or four weeks after the end of the period of rapid inflorescence production and that this coincides with the time when only a small proportion of unripe inflorescences remain.     

Effect of seed crop management on potential seed yield of contrasting white clover varieties

An experiment is described in which the effects of different spring managements on the potential seed yield and seed yield components of three white clover cultivars of contrasting leaf types were assessed. Cv. S184, the small-leaved variety, produced more but smaller inflorescences than CVS Olwen, a large-leaved variety, or Menna, a medium-leaved variety. However, cv. Olwen, produced inflorescences with more florets, seeds per inflorescence and a higher seed yield per ten inflorescences than the other cultivars. Both potential seed yield and the individual yield components were influenced by management. Cv. Olwen produced more inflorescences and a higher potential seed yield under a cutting system than under grazing systems, which reduced the number and size of the inflorescences. Cvs S184 and Menna were less influenced by management system and performed similarly under cutting and grazing. The highly significant relationship between the number of ripe inflorescences and potential seed yield showed clearly that high seed yields are only achieved if the crop is harvested when the number of ripe inflorescences is at a maximum. The relatively short duration of the period of maximum ripe inflorescences emphasized the importance of determining the correct harvesting date, although weather conditions also play an important part in deciding when to harvest. Florets per inflorescence, seed set and 1000 seed weight remained relatively constant over the harvest period, and were not influenced by harvest date. The results are discussed in relation to the management of white clover seed crops and the importance of climatic conditions during seed production.     

Nitrogen studies in Lolium perenne grown for seed. IV. Response of amenity types and influence of a growth regulator

In field trials in 1979–81, perennial ryegrass cultivars Royal and Majestic (amenity) and Morenne (agricultural) produced maximum seed yields at levels of applied N ranging from 40 kg ha⁻¹ to 160 kg ha⁻¹. Available soil N levels were estimated at 55 kg ha⁻¹; hence maximum seed yields were obtained at total nitrogen levels of 95–135 kg ha⁻¹ in Royal, 95–215 kg ha⁻¹ in Morenne, and 175–215 kg ha⁻¹ in Majestic.
Applied N at rates above 120 kg ha⁻¹ either reduced or did not significantly increase seed yield, decreased seed numbers per unit area and decreased spikelets per tiller and seeds per spikelet.
The use of a growth regulator increased seed yields by preventing lodging, but did so irrespective of nitrogen application rate. It is suggested that failure to increase seed yield at high N rates is a result not of poor pollination because of lodging, but seed abortion as a consequence of competition for assimilate supply by secondary vegetative tillers.     

Seed production in white clover (Trifolium repens L.). II. Effect of autumn defoliation on potential seed yield and seed yield components

A field experiment was conducted to assess the effect of autumn defoliation treatments on inflorescence production, potential seed yield and yield components of white clover cv Makibashiro. Between 10 July and 10 October 1992, white clover swards were subjected to one of three treatments: monthly cutting to 3–4 cm (4DEF), cutting to 3–4 cm on 10 August and 10 October (2DEF), and no cutting (control). The total numbers of inflorescences and the proportion of inflorescences in different development categories were counted throughout the period of seed crop development to determine the pattern of inflorescence development and optimum harvest date. There were consistent significant differences in inflorescence density between treatments. Plots which received the 2DEF treatment produced significantly more inflorescences than did the no-defoliation (control) and 4DEF-treatment plots. In this particular year the optimum harvest date (date at which the proportion of ripe inflorescences and potential seed yield was highest) was 26 July, approximately 30 d after peak flowering. Defoliation treatments had no effect on optimum harvest date. However, treatments differed in potential seed yield and ripe inflorescences on this date. The 2DEF treatment gave the highest potential seed yield because there were significantly more ripe inflorescences than either the contol or 4DEF plots. Control plots produced inflorescences with more florets than the other two defoliation treatments, but the differences were not always significant. Seed number per pod was higher in inflorescences obtained from previously defoliated plots than from control plots. The 1000-seed weight was significantly lower in inflorescences developed in 4DEF plots than those developed in 2DEF and control plots. The results are discussed in relation to the management of white clover seed crops and the importance of canopy structure and light intensity for seed production.     

The effect of companion perennial ryegrass cultivars on red clover productivity when timing of the first cut is varied

Replicated plots of Hungaropoly red clover were sown on a sterilized area in May 1975 alone (seed rate 11 kg ha^-1) or with one of six cultivars of perennial ryegrass (seed rate 3·5 kg ha^-1) viz. Cropper and S24 (early heading), Barlenna and Hora (medium heading) and Melle and Perma (late heading). In 1976 and 1977 primary growth was cut at one of four dates ranging from mid-May to mid-June and thereafter plots were harvested twice each year.
Varying the time of first cut did not have a significant effect on total dry matter (DM) yield in either year despite differences in means of cutting treatments on annual red clover yields of the order of 6–9%.
In some companion grass treatments total DM yield in 1976 was increased and total red clover yield and percentage red clover contribution were reduced relative to swards sown only with red clover. In 1977 a similar but non-significant trend was found. Swards containing early ryegrasses had higher total herbage DM yields but lower red clover yields and contents than all other swards at the first harvest in both years.
Delay in date of taking the first harvest in 1976 reduced DM digestibility in the first cut and increased it in the second in both years.
It is suggested that by cutting early and increasing the number of harvests from three to four per year, differences in the content of red clover between the first and second cut might be reduced, and it is concluded that more benefit is derived from red clover when medium or late heading ryegrasses are used as companion grasses.     

The effect of row spacing and cover-crop on stolon development and the seed yield components of white clover cultivars of contrasting leaf size

Three white clover cultivars, S184 (small-leaved), Menna (medium-leaved) and Olwen (large-leaved), were broadcast or sown in 15, 30 or 60 cm drills at a seed rate of 3 kg ha^-1. The three cultivars were either sown without a cover-crop or sown under a cover-crop of spring barley (cv. Crescent) or peas (cv. Countess). The effects of these methods of establishment on the stolon growth and components of seed yield were subsequently measured.
Stolon growth and development was influenced by row spacing, cultivar and season. The overall plant response at all but the widest row spacing (60 cm) was to increase stolon growth such that inflorescence production, the number and proportion of ripe inflorescences and the other seed yield components were unaffected by row spacing. Cultivars differed in their response to row spacing. Cultivar Olwen produced most inflorescences and more ripe inflorescences when broadcast, cv. S184 when sown at 60 cm row spacing and cv. Menna at 15 or 30 cm row spacing. Cultivars also differed in their response to cover-crop, with cvs Menna and S184 producing more inflorescences and more ripe inflorescences when sown under barley and peas than when pure sown. The inflorescence production of cv. Olwen was not influenced by cover-crop.
The relationship between vegetative and reproductive growth is discussed in relation to establishment, cultivar and climate and the possible implications for the establishment of white clover seed crops in the UK.     

Breeding and evaluation of new white clover varieties for persistency and higher yields under grazing

Two experiments were established in 1989 and 1990 to evaluate the performance of white clover ( Trifolium repens ) varieties under grazing, using sheep and cattle, and also under a 'cutting-only' regime. The three potential medium/large-leaved varieties (AberVantage, AberCrown and AberDai) were selected and evaluated in a breeding programme that emphasized improving yield and persistency under grazing.
In Experiment 1, the potential varieties were sown with two different grass companions, and evaluated under a continuous cattle-grazing system, representative of their future role in actual farm practice, i.e. a set stocking system. Clover yield of the potential varieties in the second harvest year averaged 1–5 t ha⁻¹ more than the control variety Olwen. Total sward production of AberVantage, AberCrown and AberDai was 2.0–1.4 t ha⁻¹ higher than that of Olwen.
In Experiment 2, clover yields of the three potential varieties averaged 2.1 t more than Olwen in the second harvest year under continuous sheep grazing, but all three were lower yielding than Olwen in the cutting regime.
Both experiments illustrate how the use of grazing treatments during breeding and evaluation can improve the role of white clover varieties.     

Productivity of mixtures of Italian ryegrass and red clover

Two red clover ( Trifolium pratense ) cultivars, Red Head (tetraploid) and Kuhn (diploid), were sown at a seed rate of 13 kg ha⁻¹ either alone or in mixture with Italian ryegrass ( Lolium multiflorum ) cv. RvP sown at seed rates of 10, 15, 20 or 30 kg ha⁻¹. RvP was also sown alone at a seed rate of 30 kg ha⁻¹ and received nil or 300 kg ha⁻¹ fertilizer a⁻¹ fertilizer N. All plots were established using the barley cultivar Midas sown at a seed rate of 100 kg ha⁻¹ as a nurse crop.
Neither clover cultivar nor ryegrass seed rate significantly influenced either dry matter harvested or botanical composition over the 3 harvest years. On average over all years the grass-clover mixtures produced 75% of the yield of the N-fertilized RvP, 125% of the clover monocultures and 225% of the unfertilized RvP. The red clover contribution to the total dry matter harvested of the mixtures averaged 45–60%. The dry matter concentrations of the mixtures were considerably higher than those of the pure clover stands. In the third year yields were markedly reduced in comparison with those in the first and second years.
It was concluded that Italian ryegrass can be a suitable companion grass for red clover. Its superior yielding capacity over other grasses such as perennial ryegrass or timothy under a conservation management can be coupled to advantage with red clover to give a sward which Is essentially stable, at least over a 2- to 3-year cropping period, although giving slightly reduced yields in the third year. Italian ryegrass-red clover mixtures, without the use of fertilizer N, can produce high DM yields of good quality herbage.     

Spring defoliation of white clover seed crops. 2. Potential harvestable seed yield and seed yield components of contrasting white clover cultivars

The effects of different spring defoliation managements on potential harvestable seed yield and seed yield components of three contrasting white clover cultivars were assessed. The small-leaved cv. S184 produced more but smaller inflorescences than the large-leaved cv. Olwen and Menna, a medium-leaved cultivar. Cultivar Olwen, however, produced more ripe and brown (nearly ripe) inflorescences with more florets, seeds per floret and a higher seed yield per ten inflorescences than the other cultivars. Potential harvestable seed yield and individual seed yield components were only influenced by defoliation after bud emergence, as defoliation before bud emergence had no effect on seed yield components. Defoliation after bud emergence had a similar effect on all cultivars: the number of ripe inflorescences was unaffected by defoliation but the number of brown and therefore harvestable (ripe + brown) inflorescences was highest following defoliation three weeks after bud emergence. Florets per inflorescence, seed per floret, 1000 seed weight, seed yield per ten inflorescences and potential harvestable seed yield were not influenced by defoliation after bud emergence. Season had a significant effect on seed yield components and influenced the effect of defoliation treatments, emphasizing the importance of climate in white clover seed production. The results are discussed in relation to the spring defoliation of white clover seed crops, harvesting techniques and the provision of guidelines for optimizing seed yield.     

Flowering and true seed production in potato (Solanum tuberosum L.). 1. Effects of inflorescence position,nitrogen treatment,and harvest date of berries

Summary Flowering and true seed production from different order inflorescences in potato (Solanum tuberosum L.) were evaluated in two experiments. The number of flowers per inflorescence, berry set, berry weight, number of seeds per berry and seed weight generally decreased from primary inflorescences to tertiary inflorescences and inflorescences on lateral stems. The possible relation with carbohydrate distribution is discussed. Quality of the seeds produced from the different orders of inflorescences was a function of seed size; larger seeds showed better germination, emergence and seedling growth. Late harvest of berries increased berry weight and number of seeds per berry from primary inflorescences. Application of additional nitrogen during the flowering period did not affect flowering or differences between inflorescence positions in seed production, but it significantly decreased the number of seeds per berry. The practical implications of these results for true seed production are discussed.     

THE EFFECT OF RELATING APPLICATIONS OF NITROGEN TO THE STAGE OF DEVELOPMENT OF COCKSFOOT (DACTYLIS GLOMERATA L.) GROWN FOR PRODUCTION OF SEED

An experiment is described in which nitrogen was applied in relation to the physiological stage of development in cocksfoot grown for seed. All applications of N increased the yield of seed, but applying all the N during the phase of autumnal tillering was less effective than applying it at stages of development in spring (beginning of vernal growth, initiation of inflorescences, complete differentiation of inflorescences), or in autumn and spring, because of a decrease in the number of tillers which survived to flower in the following year. Differences due to the stage of development at which N was applied in spring were small. Primary effects of N were to increase the number of tillers and ears per plant, and to accelerate the rate of development of inflorescences; in addition, N advanced the date of initiation of inflorescences without similarly advancing the date of complete differentiation of inflorescences. It is concluded that a long period between the initiation and complete differentiation of the inflorescence is conducive to high yields of seed.     

The selection of winter wheat varieties for whole-crop cereal conservation

Ten commercial varieties of winter wheat were sampled at different stages of growth, and dry mailer (DM) yields and potential nutritional quality, as determined by modified acid detergent fibre concentration (MADF), were measured. Maximum yields for the different varieties were spread over a four-week period, when the DM content was above 350 g kg⁻¹. When the varieties were harvested for conservation as either fermented (DM 380 g kg⁻¹) or alkaline (DM 580 g kg⁻¹) whole crop, the mean MADF concentration was similar at both harvests, with large differences recorded between varieties. At both harvests the quality of the crops was sharply increased by raising the cutting height from 10 to 40 cm above ground level.     

Effect of seed crop management on the potential seed yield of contrasting white clover varieties.

Three contrasting white clover varieties, Olwen (large-leaved), Menna (medium-leaved) and S184 (small-leaved) were sown with and without a grass companion in 30 cm drills. Two grazing and one mechanical spring defoliation managements were imposed. Counts of the total number of inflorescences and of the percentage in various ripeness categories were made throughout the period of seed crop development to determine the pattern of inflorescence development and optimum harvest date. Inflorescence numbers were greatest in cv. S184 and least in cv. Olwen and, in general, varieties produced both more inflorescences and a greater proportion of ripe inflorescences when grown under spring defoliation managements similar to those for which they were bred. Thus, cv. Olwen produced more ripe inflorescences, and a lower proportion of brown inflorescences (semi-ripe), after mechanical defoliation while cv. Menna produced more ripe inflorescences following mechanical defoliation and rotational grazing. However, cv. S184 produced more ripe inflorescences under both grazing managements than under mechanical defoliation. Optimum harvest date was not affected by management or variety, total inflorescence number and maximum ripe inflorescences having reached a peak on 11 September. However, varieties differed in the proportion of ripe and brown (semi-ripe) inflorescences on this date, with cv. Menna and cv. S184 containing a significantly higher proportion of brown inflorescences than cv. Olwen, The implications of these differences in inflorescence development, the proportions of inflorescences in the various ripeness categories and their contribution to seed yield are discussed in relation to the management of white clover seed crops and harvesting method under UK climatic conditions.     

Cattle slurry as a source of nutrients for red clover: herbage production and clover contribution

An established sward of red clover cv. Hungaropoly sown pure received approx. 30 kg P ha^-1 and 200 kg K ha^-1 each year for 3 successive years. The P and K were applied either as cattle slurry, inorganic fertilizer or combinations of these. Treatments were applied either in spring or after the first harvest. There were a total of six treatments and these were harvested three times each year. The average yields of total herbage DM over all the treatments in the first, second and third years were 15·2, 14·2 and 14·2 t ha^-1 respectively and the various treatments had no significant effect on the overall yields.
Treatments had a significnt effect on red clover DM yields and percentage red clover in one harvest in each of the first 2 years and all three in the third year. Yields of red clover were lower and grass higher in treatments receiving cattle slurry only. On this treatment there was a total yield of 23·2 t ha^-1 red clover DM in the 3 years compared with 30·2 t ha^-1 on the inorganic fertilizer treatments. However, by applying P and K fertilizer in the spring, followed by cattle slurry after the first harvest, it was possible to maintain a high proportion of red clover in the sward and to produce yields of red clover DM similar to those on the inorganic fertilizer treatments.     

The effect of fertilizer nitrogen rate, white clover variety and closeness of cutting on herbage productivity from perennial ryegrass/white clover swards

Four varieties of white clover (small-leaved cv. Aberystwyth S184. medium-leaved cv. Grasslands Huia and large-leaved cvs Linda and Olwen) were sown at 3 kg ha^-1 together with 10 kg ha^-1 perennial ryegrass cv. Talbot. Herbage productivity was measured for three harvest years, 1979-81, over four annual rates of fertilizer N (0,120,240 and 360 kg ha^-1) and two closeness of cutting treatments (80 and 40 mm from ground level). A simulated grazing regime of six cuts per year at 3- to 6-week intervals was used.
Production of total herbage DM was increased by increasing N rate; mean annual DM production ranged from 783 1 ha^-1 with no N to 11701 ha^-1 at 360 kg ha^-1 N. Mean herbage response to N (kg DM per kg N applied) was 73,90 and 108 for the three successive N increments relative to no N. Mean white clover DM production was reduced from 4 14t ha^-1 with no N to 051 t ha^-1 at 360 kg ha^-1 N.
The large-leaved clover varieties were more productive than the small- or medium-leaved varieties at all N rates. Close cutting increased total herbage and white clover by a mean annual 16% and 31%. respectively. White clover varieties did not interact with either N rate or closeness of cutting.
It is concluded that repetitive N application over the growing season is incompatible with white clover persistence and production, even with large-leaved clover varieties or with close cutting, two factors which improved clover performance in the experimental swards.     

The effects of density, date of inflorescence emergence, date of harvesting and temperature upon seed quality in tetraploid hybrid ryegrasses

Thousand-seed weight, germination and seedling dry weight were measured in some tetraploid hybrid ryegrasses to study variations in seed quality. In cv. Sabrina, seed from spaced plants had a higher 1000-seed weight and seedling dry weight, and a slower germination rate than seed from narrow drills. Seed from later emerging groups of inflorescences had a low 1000-seed weight and seedling dry weight, but a high germination rate. Seeds of cv. Sabrina, Leri and Augusta harvested at between 10 and 25 d after peak anthesis had low 1000-seed weights, low germination rates and low seedling dry weights. Air temperatures between inflorescence emergence and seed maturity also affected seed quality; an increase in temperature from a 15°/10°C regime to a constant 25°C environment reduced 1000-seed weight and seedling dry weight, but increased germination rate. It is concluded that year-to-year variation in seed characters will occur because of temperature and other climatic changes, but seed quality can also be influenced by the time at which the seed is harvested. If combine harvesting is carried out at a moisture concentration of about 400 g kg^-1 (≡40%) then maximum yields of seed of a high quality should be obtained.     

Establishment and production of common sainfoin (Onobrychis viciifolia Scop.) in the UK. 1. Effects of sowing date and autumn management on establishment and yield

The effects of sowing date and autumn management of sainfoin ( Onobrychis viciifolia Scop.) were investigated over 3 years in the UK. Replicated plots were sown between April and September in 2003 and 2004. Autumn management treatments were early and late cutting carried out in the establishment year and in subsequent years. Dry matter (DM) yields were measured over 3 years. One harvest was taken from April to July sowings in the establishment year and three harvests in each of the following years. DM yields from sowings in April and May were 2·8 and 3·3 t DM ha⁻¹, respectively, in the establishment year, which were higher ( P  <   0·001) than from sowings in June and July. Sowings from April to July yielded 10·9–12·5 t DM ha⁻¹ in the first full-harvest year, and 9·5–11·5 t DM ha⁻¹ in the following year. Sowings in August and September only gave 5 t DM ha⁻¹ year⁻¹. Early-autumn cutting of an established sward reduced yields of sainfoin at the second harvest in the first and second full-harvest years. Sowing in May had the lowest proportion of weed species (0·06) in the establishment year, and sowing in July had the highest (0·53). Crude protein concentration increased as the seasons progressed from 149·8 to 230·1 g kg⁻¹ DM.