平欧杂种榛子品种栽培比较试验

<正>平欧杂种榛子是由我国原产的平榛与欧洲榛种间远缘杂交选育出的优良品种,集中了欧洲榛与平榛的优良遗传基因,既具有平榛的抗寒适应性强、风味好的特点,又具有欧洲榛的大果、丰产、出仁率高的特点。辽宁省桓仁县2003年开始对平欧杂种榛子优良品种进行栽培,经过11年的栽培试验、技术改进,筛选出抗寒性、丰产性都好的品种。1材料与方法试验地点在桓仁满族自治县五里甸子镇老黑山村4组,共有8个地段,面积依次为6 600、6 020、6 734、     

杂交榛子‘辽榛7号’‘辽榛8号’‘辽榛9号’的选育

<正>平榛原产我国,野生资源丰富,分布广泛,抗寒性强,但果小、壳厚、产量低。引进的欧洲榛果大、壳薄、丰产性好,但抗寒力弱,适生区域狭窄。培育适合我国栽培的大果、优质、丰产、抗寒的榛子新品种,是榛树育种的主要目标~([1])。辽宁省经济林研究所利用平榛和欧洲榛种间远缘杂交育种的方法,已培育出一批杂交榛子优     

平欧杂交榛扦插育苗技术

正平欧杂交榛是辽宁省经济林研究所于1996~1999年用平榛与欧洲榛杂交选育出的榛子新品种,1999年通过品种审定。遵化市于2011年开始引入进行栽培试验,经4年的栽培观察,在遵化生长发育良好,开花结果正常,丰产性强,是适合遵化市发展的新型干果优良品种。现简单介绍其扦插繁育技术。榛树是难生根树种,硬枝扦插在国内尚未取得满     

榛子新品种‘辽榛2号’

 ‘辽榛2号’为平榛与欧洲榛的种间杂种,该品种在产量、出仁率、坚果大小等方面较平榛有极显著提高,其抗寒性和栽培适应性与平榛相近,与同期选育出的‘辽榛1号’品种相比,其树姿较直立。     

售大果榛子苗

<正>大果榛子是我国平榛与欧洲榛的杂交品种,果大、皮薄、口感好,营养丰富,抗逆性强。栽培管理简单,丰产、稳产,666.7 m2效益在5 000元以上。地址:辽宁省普兰店市星     

平欧杂交榛子育苗及丰产栽培技术

欧洲榛子栽培已有700多年的历史,土耳其、意大利、西班牙、美国等发达国家榛子栽培已实现了集约化、良种化,果实个大、皮薄、产量高。我国育种工作者利用野生平榛为母本,欧洲榛为父本进行种间远缘杂交选育出一系列优良品种和品系,统称平欧杂交榛子,既继承了欧洲榛的坚果大(平均     

平欧杂种榛丰产栽培技术

平欧杂种榛是辽宁省经济林研究所于1 980～ 1 985年用我国原产的平榛 (Corylushet erphyllaFisch)与引入我国的欧洲榛 (CorylusavellanaL )进行种间远缘杂交 ,于 1 999年育成并向生产上推广的系列优良品种和杂交优系。它集中了欧洲榛与平榛的优良性状 ,既具有平榛抗寒、适应性强 ,又具有欧洲榛大果、丰产、出仁率高的特点 ,而且营养丰富 ,用途广 ,价格高 ,市场需求量大 ,发展前景广阔。目前已在河北、山东、河南、山西、新疆、四川、云南等地试栽 ,效果良好。1　榛园建立1 1　优良品种、品系性状1 )平欧 586号 :坚果长圆形 ,果大 ,平均单…     

售大果榛子苗

正大果榛子是我国平榛与欧洲榛的杂交品种,果大、皮薄、口感好,营养丰富,抗逆性强。栽培管理简单,丰产、稳产,666.7 m2效益在5 000元以上。地址:辽宁省普兰店市星台镇元岭村元岭屯联系人:高旭东电话:0411-83316150     

辽榛3号在新疆伊犁的栽培表现

<正>榛子是世界著名四大干果之一,也是我国北方重要干果,其果仁营养丰富,既可鲜食也可加工,经济价值高。我国食用榛以毛榛和平榛为主,坚果小,产量低,品质差。由我国原产平榛与欧州榛种间远缘杂交选育出的平欧杂种榛,既具有平榛抗寒适应性强、风味优良的特点,又具有欧洲榛果个大、丰产、出仁率高的特点,且因其根系发达,枝繁叶茂,还具有很好的生态保护作用。辽榛3号(原代号84-226)是辽宁省经济林研究所1984年以平榛优良单株作母本、欧洲     

杂交榛子早期丰产栽培技术

杂交榛子是平榛与欧洲榛种间远缘杂交获得的优良品种.桓仁县榛子果品生产基地于2003年引进并进行早期丰产栽培试验,收到了良好的效果.……     

我国榛属植物种质资源的研究、利用与创新

榛子为榛科榛属植物,全世界有16种,我国原产8个种和2个变种;我国现有榛林的95%是野生平榛,选育的平欧杂种榛和引进的欧洲榛是目前主要的栽培种。从平榛资源调查和选优、平欧杂种榛种质创新和品种选育、我国其他榛属资源的利用和国外榛属资源的引种等方面对我国榛子的育种现状进行了介绍;对我国及国外榛属植物种质资源的研究进展进行了总结,对比了我国与世界先进水平的差距;并从现有平欧杂种榛在科研和生产中亟待解决的问题、第1代平欧杂种榛的目标性状改良及今后榛属植物的育种策略等方面,对榛属育种与资源利用提出了一些见解,以期与国内同行专家进行交流。     

平欧杂交榛转录组中SSR信息分析和引物筛选

对平欧杂交榛的转录组数据进行了深入挖掘,以期开发其SSRs标记。在25 649条含SSR序列的Unigene中,共找到35 604条SSR序列;其中,单、二、三核苷酸重复为高频类型,其数量及所占百分比分别为：10 102（28.37%）、16 543（46.46%）、7 035（19.76%）;在单核苷酸重复中,A/T重复最多（28.13%）;二核苷酸重复中,AG/CT（35.79%）、AT/AT（7.47%）、AC/GT（3.17%）最为丰富;三、四、五、六核苷酸重复中,比例最高的重复序列分别为：AAG/CTT（7.24%）、AAAG/CTTT（0.33%）、AAAAG/CTTTT（0.38%）、AAAAAG/CTTTTT（0.15%）。依据转录组中的SSRs序列信息,随机合成了60对引物,并对含平榛（Corylus heterophylla Fisch）、平欧杂交榛（C. heterophylla × C. avellana）、欧洲榛（C. avellana）及毛榛（C. mandshurica Maxim.）的25份材料进行了PCR扩增和毛细管电泳,找到7对多态性良好的引物,其PIC（Polymorphism Information Content）值为0.35 ~ 0.70,获得多态性条带52条;UPGMA（Unweighted Pair Group Method Analysis）聚类结果显示,上述引物可将这些种质进行准确区分。以上研究初步明确了平欧杂交榛转录组中SSR的分布特征,为其遗传连锁图谱构建等研究提供了科学依据。     

平欧杂交榛的组织培养

为了探索我国平欧杂交榛离体培养和快繁技术,以5个平欧杂交榛品种(系)的幼嫩茎段为外植体,研究其组织培养及快速繁殖技术,结果表明,(1)5月中旬为茎尖培养取材的较好时期,污染率低、萌芽率高。(2)适宜的基本培养基为DKW培养基。(3)不同基因型间的萌芽率及芽苗生长状态存在极显著差异。(4)增殖阶段较好的培养基为:DKW+TDZ1.5mg/L+IBA0.01mg/L。(5)生根培养较佳培养基为:1/2MS+NAA0.1mg/L,生根率达90%。     

The effect of various storage temperatures on peroxidase activity and protein PAGE gel electrophoresis in ‘Queen’ pineapple fruit

Mature ‘Queen’ pineapple fruit were stored for 30 days at various temperatures and afterwards examined for internal quality. A storage temperature of 8°C is at present generally accepted as the optimum. It was found that peroxidase (PO) activity is correlated with storage temperature and the appearance of storage injury symptoms. High storage temperatures of 10°C, 12°C and 16°C resulted in a high PO activity with internal browning of the core and pulp whereas temperatures of 2°C, 4°C and 6°C resulted in a low PO activity and white watery pulp. To verify these results, polyacrylamide gel electrophoresis was carried out with the proteins in the fruit. The difference in the two extreme temperatures of 2°C and 16°C resulted in a high intensity of high molecular mass proteins at 2°C and an increase in intensity of low molecular mass proteins at 16°C. There is a distinct “change over” of these protein band intensities at 8°C to 12°C. It is presumed that the correct storage temperature will be within these two limits.     

Effect of postharvest desiccation at high temperature on soluble sugar changes of two supersweet sweet corn cultivars

The alteration in the soluble sugar contents of two commercial cultivars of sweet corn, Zea mays L. Supersweet Jubilee and Sweetie, desiccated at 60°C was determined. The loss of kernel moisture under field condition was gradual, but was rapid under laboratory condition at 60°C. Constant dry weight of kernels was reached after 24 h. In both cultivars, postharvest desiccation at 60°C significantly increased raffinose and fructose contents over those of non-desiccated control. Sucrose content of kernels, however, was significantly decreased by desiccation in both cultivars. In cv. Supersweet Jubilee, raffinose, sorbitol, fructose and glucose were significantly increased in kernels contained in plastic bags held at 60°C compared with naked desiccation at 60°C. In cv. Sweetie, however, only fructose and glucose contents were increased and not raffinose, sorbitol or sucrose. It was concluded that high temperature desiccation had a profound effect on sweet corn soluble sugars which may be associated with their cell membrane protecting role.     

Effects of different diurnal temperature combinations on fruit set of sweet pepper

Trials were carried out on sweet pepper, Capricum annuum L. cultivar ‘Ma'or’ under controlled temperature conditions and natural light. In the first trial, we examined night temperatures of 15, 18, 21 and 24°C (± 1) in combination with a day temperature of 24°C, and in the second trial day temperatures of 22, 25 and 28°C (12 hours) and divided day temperatures of 28-32-28°C (4+4+4 hours) in combination with a night temperature of 18°C. The highest fruit-set was obtained at the lowest night temperature; the highest night temperature caused considerable blossom drop. The highest tested day temperature did not cause increased blossom drop.     

Optimization of agrotechniques in the cultivation of Luffa acutangula

Germination requirements, flowering pattern, planting density and growing regimes were examined for Luffa acutangula (L.) Roxb. Maximum germination (50%) was obtained at 35°C, and at 8, 12, and 45°C germination was completely inhibited. Partial removal of the seed coat increased the percentage of germination while vernalization and exposure to salinity 5=50 mM NaCl reduced it. Planting season influenced flowering pattern, with significantly more female flowers being produced in spring-summer (long days and high temperatures) than in autumn-winter (short days and low temperatures). A high yield of 44.5-47.3 Mg ha 1 was obtained for plants trained on trellises at planting densities of 10,000 and 20,000 plants ha-1. Fruits kept at low temperatures showed the least deterioration during storage, a shelf life of about two weeks being demonstrated at 4°C.     

兜唇石斛的组织培养研究

以兜唇石斛的成熟蒴果作为外植体,比较不同的培养基、激素等因素对种子发芽及试管苗生根的影响。结果表明:以成熟蒴果为外植体能够诱导成苗,种子萌芽的最适培养基为:MS+NAA 0.5mg/L+6-BA 2.0mg/L+GA 1.0mg/L+香蕉汁150g/L+蔗糖20g/L+琼脂8g/L+活性炭2g/L,适宜的生根培养基为:1/4MS+NAA 2.0mg/L+6-BA 0.5mg/L+香蕉汁150g/L+蔗糖20g/L+琼脂8g/L+活性炭2g/L。     

平欧杂种榛钙调蛋白基因ChaCaM的克隆与表达分析

以平欧杂种榛（Corylus heterophylla × C. avellana）品种‘达维’为材料,基于转录组测序数据,利用RACE技术克隆钙调蛋白基因,通过生物信息学进行序列预测分析,并利用实时荧光定量PCR技术分析其在不同组织和自交不亲和授粉后不同时间雌蕊中的表达模式。克隆获得一个钙调蛋白基因序列,命名为ChaCaM（GenBank登录号：KY211037）,其开放阅读框为450 bp,编码149个氨基酸,分子量16.8476 kD。生物信息学分析表明,ChaCaM为酸性稳定蛋白,属于疏水性蛋白,无跨膜域和信号肽存在,主要定位于细胞质基质中,存在糖基化和磷酸化位点。序列分析表明,ChaCaM包含两个EFh结构域,与其他物种的CaM高度相似。系统进化树表明,ChaCaM与烟草、可可和萝卜等的CaM亲缘关系极近,聚类在同一分支下。基因表达分析表明,ChaCaM在平欧杂种榛不同组织中的表达具有差异,且在自交不亲和授粉后的雌蕊中具有明显的表达量变化。ChaCaM具有典型的钙调蛋白基因特征,主要定位于细胞质基质中;表现为组成型表达,推测其参与了榛属植物的自交不亲和反应。