The dynamics of Imperata: historical overview and current farmer perspectives, with special reference to South Kalimantan, Indonesia

The paper reviews modern attitudes to and uses of imperata grasslands in parts of Southeast Asia, including government and NGO efforts at reforestation. It is argued that the perspective of small farmers has usually been inadequately considered in planning the rehabilitation of Imperata. A historical overview of the impact of changes in land use, population, vegetation dynamics and human adaptation on the creation, disappearance or persistence of Imperata provides a context for the present concerns and emphasizes the wide variability of the situation at the local level. Nine questions are proposed as needing consideration whenever changes are planned to a grassland-based system. They cover three main areas: 1) the origins, past and present uses of the grasslands (including the present land tenure position and variations in uses by ethnic group, socio-economic status and gender); 2) perceptions by farmers of the opportunities and constraints provided by local micro-environments, particularly vegetation types; 3) farmer suggestions for ways of improving their economic status and the potential benefits of agroforestry innovations. These questions are applied to one case study area, the Riam Kiwa valley of South Kalimantan, Indonesia.     

Soil fertility management for reclamation of Imperata grasslands by smallholder agroforestry

Imperata cylindrica grasslands are widely believed to indicate poor soil fertility. Soil fertility improvement may have to be an important component of a reclamation strategy. Data for Sumatra, Indonesia indicate, however, that Imperata occurs on a broad range of soil types and is not confined to the poorest soils. A direct role of Imperata in soil degradation cannot be ascertained. In many instances, however, Imperata soils are low in available P and effective N supply. The use of rock phosphate in combination with erosion control (fertility traps) and legume cover crops can be effective in restoring soil fertility. Case studies for a number of sites in Sumatra have confirmed the practical possibility of reclaiming grasslands for food and tree crops.     

Early effects of four fast-growing tree species and their planting density on ground vegetation in Imperata grasslands

Ground vegetation development was studied under young plantations of Acacia mangium, A. crassicarpa, Gmelina arborea and Paraserianthes falcataria on an Imperata grassland site in South Kalimantan, Indonesia. The study was based on two separate trials: 1) a species trial with a spacing of 3×3 m, and 2) a spacing trial with progressively decreasing planting density. Both trials were established by use of intensive site preparation, NPK-fertilization, and weeding during the first year. Two years after planting and one year after the last weeding, ground vegetation in the first trial was dominated by Imperata cylindrica grass and shrubs Chromolaena odorata and Clibadium surinamense. In the open area (control) only Imperata grass appeared. Significant differences in total ground vegetation biomass (t ha^–1) between the tree species were as follows: G. arborea (0.2) A. mangium (1.4) A. crassicarpa (4.2) < P. falcataria(9.6) open area (11.4). The early results from the spacing trial showed similar trends in the hardwood species' abilities in Imperata suppression. Highly significant linear relationships existed in regression analysis between the distance between planted trees and the ground vegetation biomass as follows: G. arborea (r²=0.75), A. mangium (r²=0.95), A. crassicarpa (r²=0.97), P. falcataria (r²=0.96). Tree species and their planting density should thus be critically considered in forest plantation establishment on Imperata grasslands, since ground vegetation development plays a decisive role in fire susceptibility, maintenance requirements and the promotion of native species in plantations.     

Indigenous livelihood systems in industrial tree-plantation areas in West Kalimantan, Indonesia: Economics and plant-species richness

An integrated tree plantation approach combining indigenous livelihoods and industrial wood production is being implemented in West Kalimantan, Indonesia. In this study the economics of land-use was investigated in villages within the plantation scheme. The effect of three alternative land-use scenarios and costs and benefits of each land-use type were studied using linear programming. The scenarios were based 1) on the current land-use, 2) on the integrated tree plantation system with incentives and government regulations, and 3) on a financially optimal land-use distribution. Additionally, plant species richness in different land-use types was surveyed. The scenarios had different kinds of effects on villages varying in respect to their possibilities for land-use intensification. In villages with extensive land use, establishment of tree plantations without any land-use regulations increased the potential economic return on land, resulting in conversion of natural and man-made semi-natural forests into swidden fields. In villages with more sedentary agriculture, the plantation scheme did not create pressures towards remaining forests even in the case of financially optimal land-use distribution. Incorporation of land-use regulations prevented further deforestation but also decreased households' economic returns. The results also showed that conversion of Imperata grasslands to any other land-use increased species richness. Incentives, restrictions or regulations aiming at conserving natural and semi-natural forests are needed to ensure conservation of biodiversity and long-term improvements in local livelihoods. It can be concluded that tree plantations can be combined with other land-use practices: They can improve the economic return on land without further degradation of the environment.This revised version was published online in November 2005 with corrections to the Cover Date.     

Fire management on Imperata grasslands as part of agroforestry development in Indonesia

Fire is an important factor in the Imperata grassland ecosystem. It prevents or slows down the natural succession to shrubs and/or secondary forest vegetation and is a major threat to (agro)forestry options for Imperata grassland rehabilitation. Forest fires can also be a primary cause of the extension of Imperata grasslands. In this review an attempt is made to integrate biophysical and socioeconomic aspects of the causation of fires in a conceptual model. Fire effects on vegetation are examined. The management options at the level of a farmer, a village community and a national government are analyzed.     

Imperata economics and policy

Should policymakers — or anyone else — care about millions of ha of Imperata grasslands? The answer depends on the balance between costs of conversion to other uses and the net benefits produced in economic growth, poverty alleviation, and protection of the environment. The first section on Imperata economics sets up the analytical framework to address this question and draws on the wider development economics literature to consider whether growth and poverty alleviation are conflicting or complementary objectives. Although evidence is limited, it suggests smallholder-based agroforestry could provide the same economic growth with greater poverty alleviation than large-scale forestry estates. There is, however, no substitute for project appraisal for specific settings. The second section on Imperata policy reviews whether policy distortions and market failures provide a sufficient rationale for direct policy intervention to promote tree planting on Imperata grasslands. Estimates of imputed values of carbon sequestration to alleviate global warming are presented for Acacia mangium and rubber agroforestry. The conclusion summarizes the policy research agenda and examines the desirability and feasibility of policy intervention to promote carbon sequestration through Imperata grassland conversion to tree-based systems.     

Imperata management strategies used in smallholder rubber-based farming systems

A large proportion of global natural rubber production takes place in Southeast Asia. The majority of this rubber is produced by smallholders. Thailand, Indonesia and Malaysia are the three principal countries involved. In Malaysia and Indonesia the smallholder sector accounts for 72% and 84% of total rubber production respectively. In other countries rubber plays a significant role on a more local basis. Imperata is a serious problem for the smallholder rubber farmer in most of Southeast Asia in three respects: the high cost (labour and/or capital) of opening Imperata-infested land, its competitive effect on rubber and annual intercrops, and the fire hazard that it poses during the dry season as a major source of combustible material. The costs of Imperata to smallholders are substantial in terms of a direct loss through fire and revenue foregone or delayed as a result of competition. Most smallholders intercrop their rubber during the first 1–3 years after planting it, and during this period Imperata is reasonably well controlled. Intercropping is only feasible for a limited period because of limited soil fertility and/or the shading effect of the rubber trees. After intercropping, Imperata tends to establish itself for a few years until it too is shaded out by the rubber. This is the problem period, during which farmers practise only limited weed management, if any.A large amount of research has been done on methods of controlling Imperata, but the recommended methods have been generally spurned by smallholders unless they have been given subsidies to apply them. This paper describes the precise nature of the Imperata problem, with reference to some of the smallholder rubber-based farming systems within Southeast Asia; the different Imperata control strategies currently practised in these farming systems; and some of the constraints on the adoption of currently and recently recommended practices. It then proposes a ten-point agenda for research on Imperata control, including two ways in which current research programmes could be usefully reoriented: first, they need to take greater account of smallholder farming systems and constraints; and second they should shift their emphasis from single-method to integrated control systems.     

Prevalence and incidence of the root-inhabiting fungi, Fusarium, Cylindrocarpon and Pythium, on container-grown Douglas-fir and spruce seedlings in British Columbia

Surveys were made at the end of the 1990 and 1991 growing seasons for root-inhabiting fungi in the genera Fusarium, Cylindrocarpon and Pythium from the roots of one year-old container-grown Douglas-fir and spruce seedlings grown under greenhouse conditions. In the 1990 survey of four nurseries, it was found that 61–97% of both Douglas-fir and spruce roots were colonized with Fusarium, Cylindrocarpon or Pythium. There were significantly (p0.05) more Douglas-fir roots than spruce roots colonized by Fusarium at all nurseries, however, there were significantly (p0.05) more spruce roots than Douglas-fir roots colonized by Cylindrocarpon and Pythium. Root colonization of Douglas-fir and spruce by the three fungal genera during 1991 varied from 0–82% at three nurseries, however, only at a south coastal nursery was there significantly (p0.05) more spruce than Douglas-fir roots colonized by Cylindrocarpon. Significantly more seedlings were infected in 1990 than in 1991. In 1991, there were few significant differences between Douglas-fir and spruce, in the percentage of seedlings with colonized roots and in the percentage of growth medium colonized by the fungi. However, there were significant differences between nurseries.     

Provenance variation in growth characters of four subtropical pine species planted in Mexico

In 1995 a species/provenance test was established in the state of Puebla, Mexico to assess and compare the growth of Pinus greggii var. australis, P. maximinoi, P. patula and P. tecunumanii, including 10, 5, 11 and 4 provenances of each species, respectively. Each provenance test lot consisted of seed from 7 to 10 individual trees collected in natural stands. Range-wide samples were included in the test for P. greggii var. australis and P. patula. Material of P. maximinoi and P. tecunumanii from Guatemala, Honduras and Nicaragua, as well as Mexico was established in the test. The test site is in a mountainous area at 1440m elevation, near 20°N latitude. At 5 years of age significant differences were seen at both the species and provenance levels in survival, total height, diameter and volume. The overall test survival was 93%. Variance among species was three time greater than among provenances, comprising 25–33% of the total variation. P. maximinoi had the greatest height, diameter and volume (62dm³) followed by P. tecunumanii (53dm³). P. greggii var. australis had an average volume of 43dm³ and P. patula produced 30dm³. Although P. maximinoi had the lowest survival rate, the San Jeronimo provenance was the most productive of all 30 provenances tested; 98% survival and 73dm³ volume. This was equivalent to 2.8cm annual diameter increment and 2m in height growth per year. Significant growth differences among provenances within species indicate that potential gain exists using provenance selection.     

Susceptibility of adult trees of the endangered species Pinus armandii var. amamiana to pine wilt disease in the field

Adult trees of Pinus armandii var. amamiana (PAAm) and P. thunbergii grown in the field were inoculated with 100000 or 1000 of the nematode Bursaphelenchus xylophilus to evaluate their susceptibility to pine wilt disease. PAAm trees inoculated with 100000 nematodes started to show disease symptoms 2 weeks after inoculation, and all died within 29 weeks. Although the PAAm trees inoculated with 1000 nematodes tended to show delayed disease symptoms compared with those inoculated with 100000 nematodes, all of them died within 33 weeks after inoculation. All P. thunbergii trees inoculated with 1000 nematodes had died 6 weeks after inoculation. In the nematode-inoculated PAAm trees, death of branches distal to the nematode inoculation site was the first visible symptom, followed by the systemic discoloration of needles, whereas the whole tree wilted simultaneously in P. thunbergii trees. In nematode-inoculated PAAm trees, the period from inoculation to death was longer than that in P. thunbergii. These results suggest that adult PAAm trees are susceptible to pine wilt disease, but are less vulnerable than P. thunbergii.     

Productivity of hedgerow shrubs and maize under alleycropping and block planting systems in semiarid Kenya

A field experiment was conducted for eight cropping seasons from 1988 to 1991 in semiarid Machakos, Kenya, to compare the productivity of shrubs and crops in intercropping (alleycropping) versus block (sole) planting systems. The study, conducted in a split-plot experiment with three replications, consisted of two tree species (Leucaena leucocephala andSenna siamea syn.Cassia siamea) in the main-plots and combinations of two planting systems (alleycropping and block planting of the shrubs and maize) with five different ratios of land allocation for the shrub and crop (1000, 1585, 2080, 2575, and 0100) in sub-plots. Thus, a sole maize and a sole tree were included in the subplot treatments. The spacing between hedgerows of shrubs in intercropping was 6.7, 5, and 4 m, respectively, corresponding to 15, 20, and 25% land allocation to shrubs. The trees were pruned to 0.5 m height four times a year. Intercropped hedgerows of senna and leucaena produced 10% and 24% more biomass than their respective block planting systems. On average, leucaena produced more biomass than senna. Maize alleycropped with leucaena yielded 16% less grain than sole-crop maize, whereas senna intercropping caused hardly any maize-yield reduction. Compared with the respective sole-crop systems, leucaena intercropping did not affect land equivalent ratio (LER), whereas LER increased by 28% with senna intercropping. The different tree:crop land occupancy ratios did not affect the production of either the component species or of the total system, except that LER declined with incrreased spacing between hedgerows. It is concluded that in semiarid highlands of Kenya, leucaena and crops should better be grown in sole blocks, not in alleycropping. In the case of slow-growing species such as senna, intercropping is worthwhile to consider only if the additional labour needed does not pose a serious problem for management, and the species has fodder value.     

The performance of the leaf mulches ofLeucaena leucocephala,Flemingia macrophylla andGliricidia sepium in weed control

The performance of the leaf mulches ofLeucaena leucocephala, Gliricidia sepium andFlemingia macrophylla in weed control has been tested in two trials. The length of the period during which a mulch layer yields significantly less weedbiomass compared to the control plots is called the effective life-span of the mulch.Of the three mulch materials only that ofF. macrophylla shows promise in retarding weed development.In the second trialF. macrophylla leaf mulch was applied at rates of 3, 6 and 9 tons dry matter per ha. The effective lifespan of a mulch layer of 3 tons is between 12 and 13 weeks. The treatments 6 and 9 tons have effective life-spans of over 14 weeks.For moderate quantities (up to 5 tons of dry leaf mulch per ha) the effective life-span is estimated at about a 100 days.The value of mulching in weed control is limited to the control of weed species that multiply by seed. Regrowth originating from roots or stumps from former vegetation is unlikely to be checked by a mulch layer.The subject of this paper is part of a broader study, presently conducted in Southern Ivory Coast, on the production, agronomical value and use in alley-cropping systems of the species mentioned in the title of the paper. A final report is scheduled for publication in 1988.     

Fallow enrichment of a traditional slash/mulch system in southern Costa Rica: comparisons of biomass production and crop yield

The slash and mulch system of frijol tapado or covered bean is a pre-hispanic system of bean production found throughout much of Central America. However, land use pressures have forced farmers to shorten the traditional fallow period, thus resulting in decreasing productivity. A potential solution is to enrich the fallow by using leguminous nitrogen-fixing trees. The enriched fallow systems evaluated in this study include both single and mixed species treatments: 1) Erythrina poeppigiana; 2) Calliandra colothrysus; 3) Gliricidia sepium; 4) Inga edulis; 5) Inga edulis and Erythrina poeppigiana; 6) Inga edulis and Calliandra calothrysus; and 7) Inga edulis and Gliricidia sepium. Biomass production of the fallow vegetation is shown to be of greater quality and quantity in all fallow enrichment treatments. However, bean yields did not show a significant response to the fallow enrichment treatments.     

Degrading uplands in the rainforest region of Madagascar: Fallow biomass,nutrient stocks,and soil nutrient availability

Soil fertility restoration depends on natural fallows in the slash-and-burn system of eastern Madagascar. In the Beforona-Vohidrazana study zone, none of the fallow species are able to withstand the slashing, burning and cropping frequencies of 3–5 years. Eventually soils are abandoned for agriculture. Along the degradation sequence, this study quantifies fallow biomass, nutrient stocks and soil nutrient availability of four dominant fallow species Trema orientalis, Psiadia altissima, Rubus moluccanus, and Imperata cylindrica. At 3 years, the shrubs Psiadia and Rubus were more productive (11–14.4 t/ha aboveground biomass or AGB) than the tree species Trema (8.5 t/ha). Only after 5 years did tree productivity (24.7 t/ha) exceed that of shrubs (17–20 t/ha). Imperata’s biomass stagnated at 5.5 t/ha after 3 years. A sharp decline in fallow productivity was observed with advancing fallow cycles after deforestation. While Psiadia produced highest AGB in the second fallow cycle (C2) being 100%, C1 achieved 89% of that, C3 74%, and C4 only 29%. With the ability to propagate vegetatively and to accumulate important amounts of nutrients in roots, Rubus and Imperata, both exotic and invasive species, showed improved adaptation mechanisms towards frequent disturbances compared to the two indigenous species Trema and Psiadia. Available soil nutrients P, K, Mg were highest under forest and declined rapidly with increasing fallow cycles. Ca and pH rose momentarily in the first fallow cycle before declining with advanced soil use. Al concentrations increased steadily with time. As lengthened fallow periods are not practical, there is a need to intensify upland systems based on improved nutrient cycling, targeted inputs, fire-less land management, and land use diversification. Allowing regrading tree and bush fallows to accumulate biomass (above- and belowground carbon) will significantly improve Madagascar's greenhouse gas mitigation contribution.     

Food-crop-based production systems as sustainable alternatives for Imperata grasslands?

Purely annual crop-based production systems have limited scope to be sustainable under upland conditions prone to infestation by Imperata cylindrica if animal or mechanical tillage is not available. Farmers who must rely on manual cultivation of grassland soils can achieve some success in suppressing Imperata for a number of years using intensive relay and intercropping systems that maintain a dense soil cover throughout the year, especially where leguminous cover crops are included in the crop cycle. However, labour investment increases and returns to labour tend to decrease in successive years as weed pressure intensifies and soil quality declines.Continuous crop production has been sustained in many Imperata-infested areas where farmers have access to animal or tractor draft power. Imperata control is not a major problem in such situations. Draft power drastically reduces the labour requirements in weed control. Sustained crop production is then dependent more solely upon soil fertility management. Mixed farming systems that include cattle may also benefit from manure application to the cropped area, and the use of non-cropped fallow areas for grazing. In extensive systems where Imperata infestation is tolerated, cassava or sugarcane are often the crops with the longest period of viable production as the land degrades.On sloping Imperata lands, conservation farming practices are necessary to sustain annual cropping. Pruned tree hedgerows have often been recommended for these situations. On soils that are not strongly acidic they may consistently improve yields. But labour is the scarcest resource on small farms and tree-pruning is usually too labour-intensive to be practical. Buffer strip systems that provide excellent soil conservation but minimize labour have proven much more popular with farmers. Prominent among these are natural vegetative strips, or strips of introduced fodder grasses.The value of Imperata to restore soil fertility is low, particularly compared with woody secondary growth or Compositae species such as Chromolaena odorata or Tithonia diversifolia. Therefore, fallow-rotation systems where farmers can intervene to shift the fallow vegetation toward such naturally-occurring species, or can manage introduced cover crop species such as Mucuna utilis cv. cochinchinensis, enable substantial gains in yields and sustainability. Tree fallows are used successfully to achieve sustained cropping by some upland communities. A variation of this is rotational hedgerow intercropping, where a period of cropping is followed by one or more years of tree growth to generate nutrient-rich biomass, rehabilitate the soil, and suppress Imperata. These options, which suit farmers in quite resource-poor situations, should receive more attention.     

Increasing rubber planting density to shade Imperata: a bioeconomic modelling approach

The profitability to smallholders of rubber production in Imperata infested areas of Indonesia was assessed using an existing bioeconomic model. An Imperata groundcover component was incorporated within the model as follows: tree girth = f(Imperata groundcover) = f(relative shading) = f(crown height, canopy width, tree spacing) = f(tree girth). The first two relationships represent extensions to the original model.Cumulative tree girth was predicted for rubber planting densities from 400 to 1000 stems/ha. At low tree planting rates, competition from Imperata restricts tree growth. At high tree planting rates, Imperata is controlled, but there are negative consequences from inter-tree competition. These two effects of higher planting rates counterbalanced, such that tree girth was approximately constant across the range of tree densities.Tree girth is a driving force in determining latex yield. Latex yield was translated into present value net economic returns within the model, by reference to prices and costs associated with rubber production in South Sumatra, Indonesia in 1995. Economic returns from planting 400 to 1000 trees/ha were calculated. Rubber growing by smallholders on Imperata infested land, was found to be profitable. Maximum profitability was obtained at 600 trees/ha. However, the sensitivity of net economic returns, with respect to tree density, was not great. This was due to the counterbalancing effects of changes in tree density.     

Minimum distance boundary method: maximum size-density lines for unthinned <Emphasis Type="Italic">Acacia mangium</Emphasis> plantations in South Sumatra,Indonesia

A minimum-distance boundary method that will minimize the sum of distances between measured points and a fitted self-thinning lines on log–log coordinates of stand density and quadratic mean diameter was proposed in order to estimate the maximum size density line: an upper boundary of self-thinning line. The lines for A. mangium were inferred with this method using data in two areas of unthinned plantations in South Sumatra, Indonesia. Slopes of the lines were deduced as –1.63 and –1.67 within the range of 10–21cm of quadratic mean dbh. The intensity of self-thinning was examined as a rate of reduction of density in relation to dbh increment. The rates were found to be higher than the slopes in the range close to the maximum line; hence the lines inferred in this study were likely existent. Maximum basal area deduced from the size-density line was 28–30m²/ha at 12cm of dbh and then it increased up to 34–37m²/ha at 20cm of dbh.     

Soil changes induced by Acacia mangium plantation establishment: Comparison with secondary forest and Imperata cylindrica grassland soils in South Sumatra,Indonesia

Acacia plantation establishment might cause soil acidification in strongly weathered soils in the wet tropics because the base cations in the soil are translocated rapidly to plant biomass during Acacia growth. We examined whether soils under an Acacia plantation were acidified, as well as the factors causing soil acidification. We compared soils from 10 stands of 8-year-old Acacia mangium plantations with soils from 10 secondary forests and eight Imperata cylindrica grasslands, which were transformed into Acacia plantations. Soil samples were collected every 5–30 cm in depth, and pH and related soil properties were analyzed. Soil pH was significantly lower in Acacia plantations and secondary forests than in Imperata grasslands at every soil depth. The difference was about 1.0 pH unit at 0–5 cm and 0.5 pH unit at 25–30 cm. A significant positive correlation between pH and base saturation at 0–20 cm depth indicated that the low pH under forest vegetation was associated with exchangeable cation status. Using analysis of covariance (ANCOVA), with clay content as the covariate, exchangeable Ca (Ex-Ca) and Mg (Ex-Mg) stocks were significantly lower in forested areas than in Imperata grasslands at any clay content which was strongly related to exchangeable cation stock. The adjusted average Ex-Ca stock calculated by ANCOVA was 249 kg ha⁻¹ in Acacia plantations, 200 kg ha⁻¹ in secondary forests, and 756 kg ha⁻¹ in Imperata grasslands at 0–30 cm. Based on a comparison of estimated nutrient stocks in biomass and soil among the vegetation types, the translocation of base cations from soil to plant biomass might cause a decrease in exchangeable cations and soil acidification in Acacia plantations.     

Effects of a nursery CO2 enriched atmosphere on the germination and seedling morphology of two Mediterranean oaks with contrasting leaf habit

The use of an enriched CO₂ atmosphere in tree nurseries has been envisaged as a promising technique to increase productivity and to obtain seedlings with a higher root/shoot ratio, an essential trait to respond to water stress in Mediterranean-type ecosystems. In that framework, we have analyzed the effects of three levels of atmospheric CO₂ concentration (350, 500 and 700ppm) on the germination rate, growth and morphology of seedlings of two Mediterranean oaks used in reforestation programs: the evergreen Quercus ilex L. and the deciduous Quercus cerrioides Wilk. et Costa. CO₂ enrichment increased the germination rate of Q. cerrioides (from 70±7 to 81±3%) while it decreased that of Q. ilex (from 71±10 to 41±12%). Seedlings of both species increased approximately 60% their total biomass in response to CO₂ enrichment but at two different CO₂ concentrations: 500ppm for Q. cerrioides and 700ppm for Q. ilex. This increase in seedlings biomass was entirely due to an augmentation of root biomass. Considering germination and biomass partitioning, an enriched CO₂ atmosphere might not be appropriate for growing Mediterranean evergreen oaks, such as Q. ilex, since it reduces acorn germination and the only gains in root biomass occur at a high concentration (700ppm). On the other hand, a moderate CO₂ enrichment (500ppm) appears as a promising nursery technique to stimulate the germination, growth and root/shoot ratio of deciduous oaks, such as Q. cerrioides.     

Inhibition ofTrichoderma cellulase activity by a stilbene glucoside fromPicea glehnii bark

The stilbene glucoside isorhapontin (5,4-dihydroxy-3-methoxystilbene-3--D-glucoside) is the major constituent of the ethyl acetate extracts fromPicea glehnii bark. Isorhapontin inhibited the hydrolytic activity ofTrichoderma cellobiohydrolase I (CBH I) for both bacterial microcrystalline cellulose and the soluble cellooligosaccharide celloheptaitol. The inhibitory effect for celloheptaitol, however, was more drastic than that for bacterial microcrystalline cellulose. The hydrolytic activity of the CBH I core domain for celloheptaitol was also inhibited by isorhapontin to a similar extent, suggesting that the interaction between isorhapontin and the core domain of CBH I is the reason for this phenomenon. The inhibition of CBH I activity by isorhapontin showed mixed noncompetitive and uncompetitive types in a concentration of the inhibitor of less than 125M. TheK _i andK _i values were estimated to be 57.2 and 33.3M, respectively. Whereas isorhapontin strongly inhibited CBH I activity, its aglycone isorhapontigenin (3-methoxy-3,5,4-trihydroxystilbene) showed almost no inhibition. Consequently, both the stilbenic and the-glucosidic structures in isorhapontin are essential for the inhibitory effect on CBH I activity. Isorhapontin also inhibited the activity ofTrichoderma endoglucanase I for celloheptaitol, whereas almost no effect was observed for the activities of both endoglucanases II and III.An outline of this work was presented at the 49th Annual Meeting of the Japan Wood Research Society, Tokyo, April 1999