不同浓度钙营养液对烟草矿质营养吸收与积累的影响

本文采用温室水培的方法,研究了不同的钙素供应水平对烟草各种矿质养分的吸收与积累的影响。结果表明,在其它养分形态与浓度一致条件下,随着钙浓度从75mgL-1提高到900mgL-1,烟草生长量呈抛物线型变化。钙浓度为150mgL-1时烟株干物质量最大。超过150mgL-1,随钙的增加而下降,当钙素达900mgL-1(K/Ca为1/6)时,烟株生长严重受抑。随着供钙水平由低到高,烟株氮、磷、钾、锌、锰含量均呈抛物线型变化,含钙量呈直线上升关系,而镁和铜含量则随钙浓度提高而显著下降。烟株各养分积累量均随营养液钙浓度提高呈抛物线型变化,但不同养分出现最大积累量时的营养液钙浓度却不相同。不同供钙水平上烟叶中各元素含量的变化特征与全株的结果基本一致。在低钙浓度范围(75~300mgL-1),钙对钾有协合关系,增钙促进提钾,大于300mgL-1后有拮抗作用,提钙导致降钾。钙与镁、锌、铜、锰间也存在明显的交互作用。烟叶中各元素含量与积累量的变化趋势与整株基本一致。     

Characterization of the most odor-active compounds of Iberian ham headspace

Gas chromatography-olfactometry (GC-O) based on detection frequency (DF) was used to characterize the most odor-active compounds from the headspace of Iberian ham. Twenty-eight odorants were identified by GC-O on two capillary columns, including aldehydes (11), sulfur-containing compounds (7), ketones (5), nitrogen-containing compounds (2), esters (2), and an alcohol. Among them, the highest odor potencies (DF values) were found for 2-methyl-3-furanthiol, 2-heptanone, 3-methylbutanal, methanethiol, hexanal, hydrogen sulfide, 1-penten-3-one, 2-methylpropanal, ethyl 2-methylbutyrate, and (E)-2-hexenal. Nine of the 28 most odor-active compounds were identified for the first time as aroma components of dry-cured ham, including hydrogen sulfide, 1-penten-3-one, (Z)-3-hexenal, 1-octen-3-one, and the meaty-smelling compounds 2-methyl-3-furanthiol, 2-furfurylthiol, 3-mercapto-2-pentanone, 2-acetyl-1-pyrroline, and 2-propionyl-1-pyrroline.     

Computer simulation of the interactions of glyphosate with metal ions in phloem

Essential nutrients such as trace metal ions, amino acids, and sugars are transported in the phloem from leaves to other parts of the plant. The major chelating agents in phloem include nicotianamine, histidine, cysteine, glutamic acid, and citrate. A computer model for the speciation of metal ions in phloem has been used to assess the degree to which the widely used herbicide glyphosate binds to Fe(3+), Fe(2+), Cu(2+), Zn(2+), Mn(2+), Ca(2+), and Mg(2+) in this fluid over the pH range of 8 to 6.5. The calculations show that glyphosate is largely unable to compete effectively with the biological chelating agents in phloem. At a typical phloem pH of 8, 1.5 mM glyphosate binds 8.4% of the total Fe(3+), 3.4% of the total Mn(2+), and 2.3% of the total Mg(2+) but has almost no effect on the speciation of Ca(2+), Cu(2+), Zn(2+), and Fe(2+). As the pH decreases to 6.5, there are some major shifts of the metal ions among the biological chelators, but only modest increases in glyphosate binding to 6% for Fe(2+) and 2% for Zn(2+). The calculations also indicate that over 90% of the glyphosate in phloem is not bound to any metal ion and that none of the metal-glyphosate complexes exceed their solubility limits.     

Acaricidal toxicity of 2'-hydroxy-4'-methylacetophenone isolated from Angelicae koreana roots and structure-activity relationships of its derivatives

The acaricidal activities of 2'-hydroxy-4'-methylacetophenone derived from Angelica koreana roots and its derivatives against Dermatophagoides farinae, Dermatophagoides pteronyssinus, and Tyrophagus putrescentiae were examined by vapor phase and contact toxicity bioassays. In the vapor phase toxicity bioassay, 2'-methylacetophenone (1.25 μg/cm(2)) was 8.0 times more toxic against D. farinae than benzyl benzoate (10.00 μg/cm(2)), followed by 3'-methylacetophenone (1.26 μg/cm(2)), 4'-methylacetophenone (1.29 μg/cm(2)), 2'-hydroxy-4'-methylacetophenone (1.75 μg/cm(2)), and 2'-hydroxy-5'-methylacetophenone (1.96 μg/cm(2)). In the contact toxicity bioassay, 3'-methylacetophenone (0.58 μg/cm(2)) was 17.24 times more effective against D. farinae than benzyl benzoate (7.52 μg/cm(2)), followed by 2'-methylacetophenone (0.64 μg/cm(2)), 2'-hydroxy-4'-methylacetophenone (0.76 μg/cm(2)), 4'-methylacetophenone (0.77 μg/cm(2)), and 2'-hydroxy-5'-methylacetophenone (1.16 μg/cm(2)). The acaricidal activities of 2'-hydroxy-4'-methylacetophenone derivatives against D. pteronyssinus and T. putrescentiae were similar to those against D. farinae. In terms of structure-activity relationships, acaricidal activity against the three mite species changed with the introduction of hydroxyl and methyl functional groups onto the acetophenone skeleton. Furthermore, some of 2'-hydroxy-4'-methylacetophenone derivatives could be useful for natural acaricides against three mite species.     

Kinetic study of the quenching reaction of singlet oxygen by Pyrroloquinolinequinol (PQQH(2), a reduced form of Pyrroloquinolinequinone) in micellar solution

A kinetic study of the quenching reaction of singlet oxygen ((1)O(2)) with pyrroloquinolinequinol (PQQH(2), a reduced form of pyrroloquinolinequinone (PQQ)), PQQNa(2) (disodium salt of PQQ), and seven kinds of natural antioxidants (vitamin C (Vit C), uric acid (UA), epicatechin (EC), epigallocatechin (EGC), α-tocopherol (α-Toc), ubiquinol-10 (UQ(10)H(2)), and β-carotene (β-Car)) has been performed. The second-order rate constants k(Q) (k(Q) = k(q) + k(r), physical quenching and chemical reaction) for the reaction of (1)O(2) with PQQH(2), PQQNa(2), and seven kinds of antioxidants were measured in 5.0 wt % Triton X-100 micellar solution (pH 7.4), using UV-visible spectrophotometry. The k(Q) values decreased in the order of β-Car > PQQH(2) > α-Toc > UA > UQ(10)H(2) > Vit C ～ EGC > EC ? PQQNa(2). PQQH(2) is a water-soluble antioxidant. The singlet oxygen-quenching activity of PQQH(2) was found to be 6.3, 2.2, 6.1, and 22 times as large as the corresponding those of water-soluble antioxidants (Vit C, UA, EGC, and EC). Further, the activity of PQQH(2) was found to be 2.2 and 3.1 times as large as the corresponding activity of lipid-soluble antioxidants (α-Toc and UQ(10)H(2)). On the other hand, the activity of PQQH(2) is 6.4 times as small as that of β-Car. It was observed that the chemical reaction (k(r)) is almost negligible in the quenching reaction of (1)O(2) by PQQH(2). The result suggests that PQQH(2) may contribute to the protection of oxidative damage in biological systems, by quenching (1)O(2).     

Assessment of the aroma impact of major odor-active thiols in pan-roasted white sesame seeds by calculation of odor activity values

Eleven odor-active thiols, namely, 2-methyl-1-propene-1-thiol, (Z)-3-methyl-1-butene-1-thiol, (E)-3-methyl-1-butene-1-thiol, (Z)-2-methyl-1-butene-1-thiol, (E)-2-methyl-1-butene-1-thiol, 2-methyl-3-furanthiol, 3-mercapto-2-pentanone, 2-mercapto-3-pentanone, 4-mercapto-3-hexanone, 3-mercapto-3-methylbutyl formate, and 2-methyl-3-thiophenethiol, recently identified in an extract prepared from white sesame seeds, were quantitated in sesame using stable isotope dilution analyses. For that purpose, the following deuterium-labeled compounds were synthesized and used as internal standards in the quantitation assays: [2H6]-2-methyl-1-propene-1-thiol, [2H3]-(E)- and [2H3]-(Z)-2-methyl-1-butene-1-thiol, [2H3]-2-methyl-3-furanthiol, [2H2]-3-mercapto-2-pentanone, [2H3]-4-mercapto-3-hexanone, [2H6]-3-mercapto-3-methylbutyl formate, and [2H3]-2-methyl-3-thiophenethiol. On the basis of the results obtained, odor activity values (OAVs) were calculated as ratio of the concentration and odor threshold of the individual compounds in cooking oil. According to their high OAVs, particularly the 3-methyl-1-butene-1-thiols (OAV: 2400) and the 2-methyl-1-butene-1-thiols (OAV: 960) were identified as the most odor-active compounds in pan-roasted white sesame seeds. These compounds were therefore suggested to be mainly responsible for the characteristic but rather unstable sulfury aroma of freshly pan-roasted white sesame seeds.     

不同NPK配置对强筋小麦群体生物量与产量的效应研究

在关中川道灌区以强筋小麦陕253为材料,研究了不同NPK配置对其不同生育期生物产量、群体数量和产量的效应,结果表明提高施肥量可显著增加生物产量、群体数量及产量,处理平均值分别比常规处理增加11.9%~43.6%、4.1%~17.4%和8.3%,其最佳N、P、K配置依次为N135kg hm-2,P2O5和K2O各120kg hm-2、N135kg hm-2,P2O5225kghm-2,K2O120kg hm-2和N225kg hm-2,P2O5和K2O各120kg hm-2。在目前常规投肥水平下适度增加钾肥有利于生物产量的显著提高,增加磷肥和钾肥有利于群体数量的提高,增施氮钾肥有显著的增产效果。产量的最佳NPK配比较常规处理增产19.1%,其增加养分的平均产量4.44 kg kg-1,比各处理平均养分产量高1.6倍。     

Flavone C-glycosides from leaves of Oxalis triangularis

The flavone C-glycosides luteolin 6-C-(2'-O-beta-xylopyranosyl-beta-glucopyranoside) (1), apigenin 6-C-(2'-O-alpha-rhamnopyranosyl-beta-glucopyranoside) (2), apigenin 6-C-(2'-O-beta-xylopyranosyl-beta-glucopyranoside) (3), apigenin 6-C-(2'-O-(6'-(E)-caffeoylglucoside)-beta-glucopyranoside) (4), and apigenin 6-C-(2'-O-(6'-(E)-p-coumaroylglucoside)-beta-glucopyranoside) (5) have been isolated from the purple leaves of Oxalis triangularis. Compound 4 is new, while 5 has previously been isolated from Cucumis sativus after treatment with silicon and infection with Sphaerotheca fuliginea. Signal duplication in the NMR spectra of 2, 4, and 5 revealed the presence of rotameric conformers, created by rotational hindrance at the C(sp3) -C(sp2) glucosyl-flavone linkage in these flavone C-glycosides.     

Characterization of key chromophores formed by nonenzymatic browning of hexoses and L-alanine by using the color activity concept

Thermal treatment of an aqueous solution of D-glucose and L-alanine in the presence of the carbohydrate degradation product furan-2-aldehyde resulted in the formation of a variety of colored compounds, among which (Z)-2-[(2-furyl)methylidene]-5, 6-di(2-furyl)-6H-pyran-3-one (I), [E]- and [Z]-1, 2-bis(2-furyl)-1-pentene-3,4-dione (IIa/IIb), 4, 5-bis(2-furyl)-2-methyl-3H-furan-2-one (III), and (S,S)- and (S, R)-2-[4, 5-bis(2-furyl)-2-hydroxy-2-methyl-3(2H)-pyrrol-1-yl]propionic acid (IVa/IVb) as well as 2-[(2-furyl)methylidene]-4-hydroxy-5-[(E)-(2-furyl)methylidene]methyl -2H-furan-3-one (V) were successfully identified as the most intense by application of the color dilution analysis. To measure the contribution of these colorants to the overall color of the browned Maillard mixture, color activity values were calculated as the ratio of the concentration to the visual detection threshold of each colorant. By application of this color activity concept, 16.0% of the overall color of the Maillard mixture accounted for these five types of colorants, thus confirming them as key chromophores. On the basis of synthetic model experiments, the formation pathways leading to the chromophores IIa/IIb, III, and IVa/IVb were proposed.     

不同生物质炭输入水平下旱作农田温室气体排放日变化研究

在陇中黄土高原干旱半干旱区,采用小区定位试验,对不同生物质炭水平(0 t·hm~(-2)、10 t·hm~(-2)、20 t·hm~(-2)、30 t·hm~(-2)、40 t·hm~(-2)、50 t·hm~(-2))下农田土壤温室气体(CO_2、N_2O和CH_4)的日排放通量及其影响因子进行连续观测,并确定1 d中不同生物质炭处理水平下的最佳观测时间。结果表明:6个生物质炭输入水平处理下,春小麦地土壤CH_4、N_2O和CO_2通量变化趋势与气温日变化轨迹大体一致,均表现为白天排放量大于夜间,并在4:00—5:00时,出现对CH_4通量的吸收峰,以及N_2O与CO_2的排放低谷;全天内各处理CH_4平均排放通量依次为:10.14mg·m~(-2)·h~(-1)、7.82mg·m~(-2)·h~(-1)、6.57mg·m~(-2)·h~(-1)、-0.10mg·m~(-2)·h~(-1)、1.05mg·m~(-2)·h~(-1)和2.89mg·m~(-2)·h~(-1),N_2O平均排放通量依次为:288.79mg·m~(-2)·h~(-1)、201.78mg·m~(-2)·h~(-1)、157.14mg·m~(-2)·h~(-1)、112.06mg·m~(-2)·h~(-1)、154.60mg·m~(-2)·h~(-1)和164.02mg·m~(-2)·h~(-1),CO_2平均排放通量依次为:85.44 mg·m~(-2)·h~(-1)、80.91 mg·m~(-2)·h~(-1)、76.49 mg·m~(-2)·h~(-1)、65.29 mg·m~(-2)·h~(-1)、67.19 mg·m~(-2)·h~(-1)和69.10 mg·m~(-2)·h~(-1);当生物质炭输入量小于30 t·hm~(-2)时,土壤CH_4、N_2O、CO_2排放通量随其输入量增加而显著减小,但当其输入量超过30 t·hm~(-2)时,3种温室气体排放通量则呈显著增大趋势;当生物质炭输入水平为30 t·hm~(-2)时,春小麦土壤全天表现为CH_4的吸收汇,其余各水平处理下的土壤表现为CH_4的弱排放源;6种处理水平下,全天春小麦地土壤表现为N_2O、CO_2的排放源。0~5 cm的土壤温度及水分(y)与生物质炭输入量(x)回归方程分别为y=-0.017 6x+16.585(R~2=0.302 6,r=-0.55,P0.05)和y=0.056 5x+13.626(R~2=0.815 1,r=0.903,P0.05),生物质炭输入量与0~5 cm的土壤水分呈显著正相关关系;无生物质炭输入处理下3种温室气体的吸收或排放通量与地表温度及5 cm地温均呈显著正相关关系,其他各处理也表现出不同程度的正相关关系。因此,当生物质炭输入水平为30 t·hm~(-2)时,更有利于CH_4、N_2O和CO_2 3种温室气体的增汇减排;生物质炭输入水平差异引起的土壤温度及水分差异可能是不同生物质炭处理CH_4、N_2O和CO_2日排放通量产生差异的主要原因;由矫正系数及最佳时段温室气体排放量与累积排放量回归分析可得,3种温室气体的最佳同期观测时间为8:00—9:00。     

Metabolic fate of isotopes during the biological transformation of carbohydrates to 2,5-dimethyl-4-hydroxy-3(2h)-furanone in strawberry fruits

Isotopically labeled D-glucose, D-fructose, 1-deoxy-D-fructose, and 6-deoxyhexoses were applied to detached ripening strawberry (Fragaria x ananassa) fruits, and the incorporation of the isotopes into the key strawberry aroma compounds 2,5-dimethyl-4-hydroxy-3(2H)-furanone (DMHF, 1) and 2,5-dimethyl-4-methoxy-3(2H)-furanone (DMMF, 2) was determined by gas chromatography-mass spectrometry. In contrast to previous reports the data clearly showed that 6-deoxy-D-fructose/6-deoxy-D-glucose and 1-deoxy-D-fructose are not natural precursors of the furanones. However, isotopically labeled 1 and 2 were observed after the application of [1-(2)H]-, [2-(2)H]-, and [6,6-(2)H(2)]-D-glucose as well as [U-(13)C(6)]-, [1-(13)C]-, [1-(2)H]-, [6,6-(2)H(2)]-D-fructose. The isotope label of [4-(2)H]-D-glucose was not recovered in the furanones. In contrast, [2-(2)H]-D-glucose was converted to [1- or 6-(2)H]-1 and [1- or 6-(2)H]-2 by the strawberry fruits. The observed isotope shift can be explained by the catalysis of phosphohexose isomerase in the course of the biogenesis of the hydroxyfuranone (1) and the methoxyfuranone (2) from D-glucose. Thus, the applied D-glucose is metabolized to D-fructose-6-phosphate prior to the transformation into the furanones.     

施肥对喀斯特地区植草土壤活性有机碳组分和牧草固碳的影响

通过盆栽试验,研究施肥对喀斯特地区植草土壤不同活性有机碳组分和牧草固碳的影响。试验处理包括CK（不施肥）、 N1 （N 150 mg/kg）、 N2 （N 250 mg/kg）、 N1P1 （P2O5100 mg/kg）、 N2P2 （P2O5150 mg/kg）、 N1P1K1 （K2O 70 mg/kg）、 N1P1K2 （K2O 105 mg/kg）和N2P2K1和N2P2K2。结果表明,与对照（不施肥）相比,施肥处理增加植草土壤有机碳、 微生物量碳和易氧化碳,有机碳日矿化量和累积矿化量以及牧草固碳量。其中N1P1K1处理土壤有机碳和易氧化碳最高,N1P1处理土壤微生物量碳最高,N2P2K1处理土壤可溶性碳最高,N2P2K2处理牧草地上部及根系固碳量、 有机碳日矿化量和累积矿化量均最高。综上,低量氮磷钾肥配施有利于土壤活性有机碳的积累,高量氮磷钾平衡配施牧草固碳效果最佳。     

施肥对幼龄腰果植株生长和结果的影响

采用L9(34)正交设计,探讨氮、磷、钾肥配施对幼龄腰果植株生长和结果的影响.试验结果表明,对于2龄腰果植株,最佳施肥组合为N1P1K1,年施氮(N)150 g/株、磷(P2O5) 50 g/株和钾(K2O) 50 g/株处理,可以促进植株生长;对于3龄腰果植株,年施氮250～450 g/株处理,植株的冠幅、株高增量随着氮施用量增加而增大,磷、钾影响不显著;现蕾期和初花期,P2O5 90 g/株和K2O 200 g/株处理的植株现蕾、开花枝梢比例最高,施氮影响不显著;盛花坐果期,施N 350 g/株、P2O5 150 g/株和K2O 150 g/株处理的植株现蕾开花枝梢比例最高.氮和钾对3龄植株结果影响较大,年施N 250～450 g/株处理,平均株产量随着氮施用量的增加而增加,最佳施肥组合为N3P1K2,施N 450 g/株、P2O5 90 g/株和K2O 150 g/株处理,可以促进3龄腰果植株结果.3龄植株腰果产量与初花期叶片钾(K)含量(0.43%～0.47%)和盛花坐果期叶片N含量(1.89%～2.20%)呈直线显著正相关.     

Influence of cucumariosides upon intracellular [Ca2+]i and lysosomal activity of macrophages

Biological effects of the triterpene glycosides, cucumariosides A(2)-2 and A(7)-1 from the edible sea cucumber Cucumaria japonica and their aglycones were investigated using embryos of the sea urchin Strongylocentrotus nudus and the BALB/C line mouse peritoneal macrophages. Cucumariosides were highly cytotoxic in a sea urchin embryo development test with EC(50) values of 0.3 and 1.98 microg/mL, respectively. The aglycone was completely lacking in cytotoxicity. In subtoxic concentrations (0.001-0.1 microg/mL), cucumarioside A(2)-2 showed more then 2-fold stimulation of lysosomal activity and induced a rapid short-term increase in cytosolic Ca(2+) content in mouse macrophages. The maximal stimulatory effect was detected after 1-2 h of cultivation of cells with this glycoside. Cucumarioside A(7)-1 demonstrated more weak effects and even slightly inhibited lysosomal activity, while the aglycone was completely ineffective. At the toxic concentration (1 microg/mL), cucumarioside A(2)-2 induced the sharp irreversable increase of intracellular Ca(2+) concentration. We suggested that cucumariosides, especially A(2)-2, may act as Ca(2+) agonists due to their membranolytic properties.     

Structural elucidation of phototransformation products of azimsulfuron in water

The photodegradation of the sulfonylurea herbicide azimsulfuron, N-[[(4,6-dimethoxypyrimidin-2-yl)amino]carbonyl]-1-methyl-4-(2-methyl-2H-tetrazole-5-yl)-1H-pyrazole-5-sulfonamide (AZS), was studied in water at different wavelengths and in the presence of photocatalysts. AZS was rapidly degraded by UV light, affording three photoproducts. The main product, accounting for about 70% of photodegraded herbicide, was identified as 6-amino-5-[(4,6-dimethoxypyrimidin-2-yl)methylamino]-1,5,6,8-tetrahydro-7-oxa-8lambda(6)-tia-1,2,5,6-tetraza-azulen-4-one (ADTA) by single-crystal X-ray diffraction. With simulated sunlight irradiation, the reaction was slower and 2-amino-4,6-dimethoxypyrimidine (DPA) and 1-methyl-4-(2-methyl-2H-tetrazole-5-yl)-1H-pyrazole-5-sulfonamide (MPS), arising from a photohydrolytic cleavage of the sulfonylurea bridge, were the only byproducts observed. The reactions followed first-order kinetics. The addition of dissolved organic matter (DOM) did not modify significantly the AZS photodegradation rate. The presence of Fe2O3 accelerated more than twice the reaction rate affording two major products, DPA and MPS, together with minor amounts of N-[[(5-hydroxy-4,6-dimethoxypyrimidin-2-yl)amino]carbonyl]-1-methyl-4-(2-methyl-2H-tetrazole-5-yl)-1H-pyrazole-5-sulfonamide (AZS-OH). The greatest degradation rate was detected in the presence of TiO2. Only the photohydroxylation product AZS-OH was observed, which was transformed rapidly into oxalic acid.     

温度和二氧化碳浓度升高对青稞生长的影响

采用开顶式气室（OTC）模拟温度和二氧化碳（CO2）浓度升高的生长环境,以模拟大田环境为对照（CK）,通过大田原位模拟试验对“藏青2000”青稞品种在增温2℃（T2）、增温4℃（T4）和增温2℃同时增加CO2浓度100μL·L-1（T2+CO2）环境下进行观测,分析气候变化环境下青稞的生育期、光合作用、农艺性状及其耕层土壤养分的变化。结果表明：增温处理（T2、T4、T2+CO2）下青稞的生育期缩短5～7d。其中,T4处理的生育期最短,为92d,T2和T2+CO2处理为94d。增温处理中青稞叶片光合作用降低,其净光合速率（Pn）、蒸腾速率（Tr）和气孔导度（Gs）均显著低于CK。增温处理使土壤耕层有机质和速效钾含量显著降低,速效磷含量提高。与CK相比,T2、T4和T2+CO2各处理青稞产量分别下降31.4%、46.9%和39.4%;增温2℃使青稞农艺性状（株高、干物质量、穗长）有所提高,在增温的基础上增加CO2浓度对青稞的影响并不显著,增温4℃严重影响青稞的正常生长和产量。     

洞穴水Ca2+,Mg2+含量特征及其对次生化学沉积物发育影响--以重庆武隆芙蓉洞为例

洞穴水Ca^2＋，Mg^2＋含量及其变化量是洞穴次生化学沉积物发育程度的最直接和最基础的反演载体。通过对芙蓉洞地表水、基岩水及洞穴水进行采样对比分析认为：（1）芙蓉洞洞穴水Ca^2＋，Mg^2＋含量总体变化不大，Ca^2＋离子含量为44～52mg／L．Mg^2＋离子含量为32～45mg／L。洞穴次生化学沉积物的主要物质来源是Ca^2＋离子．正处于Ca^2＋离子沉淀较为完全，而Mg^2＋离子还没来得及甚至没有开始沉淀阶段。（2）碳酸盐岩地区次生化学沉积物Ca^2＋／Mg^2＋比值在一定程度上反映了其景观发育成熟度。不同岩溶地区的岩石渗透性在很大程度上影响岩溶洞穴次生化学沉积物的发育程度及速率。（3）洞穴水Ca^2＋,Mg^2＋离子含量与洞穴次生化学沉积物发育特征关系不大。（4）10年来，芙蓉洞洞穴水Ca^2＋离子含量变化，在很大程度上影响次生化学沉积物发育度。和Mg^2＋离子关系不大。外界能量输入引起的温度升高对次生化学沉积物发育程度影响较小，而pH值的改变对它影响较明显。     

Pathway of dephosphorylation of myo-inositol hexakisphosphate by phytases of legume seeds

Using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytases purified from faba bean and lupine seeds, respectively, was established. The data demonstrate that the legume seed phytases under investigation dephosphorylate myo-inositol hexakisphosphate in a stereospecific way. The phytase from faba bean seeds and the phytase LP2 from lupine seeds degrade phytate by sequential removal of phosphate groups via D-Ins(1,2,3,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2) to finally Ins(2)P, whereas the phytases LP11 and LP12 from lupine seeds generate the final degradation product Ins(2)P via D-Ins(1,2,4,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2).     

Preparation and biological activity of four epiprogoitrin myrosinase-derived products

(5R)-5-Vinyl-1,3-oxazolidine-2-thione, (2S)-1-cyano-2-hydroxy-3-butene, and two diastereoisomeric erythro-(2S)- and threo-(2S)-1-cyano-2-hydroxy-3,4-epithiobutanes were prepared in pure form starting from (2S)-2-hydroxybut-3-enyl glucosinolate (epiprogoitrin). This glucosinolate was isolated in almost pure form using ripe seeds of Crambe abyssinica and then hydrolyzed under different conditions. The hydrolysis was carried out using either myrosinase immobilized on nylon, to produce (5R)-5-vinyl-1,3-oxazolidine-2-thione, or the endogenous myrosinase contained in defatted crambe meals, to produce the other epiprogoitrin-derived products. After purification and physicochemical characterization, all four myrosinase degradation products were tested for their biological activity. A bioassay on Lactuca sativa was chosen as a simple test to determine their apparent action on living tissues. (5R)-5-Vinyl-1,3-oxazolidine-2-thione negatively affected mainly root growth, whereas (2S)-1-cyano-2-hydroxy-3-butene affected the early phase of germination, and both (2S)-1-cyano-2-hydroxy-3,4-epithiobutane diastereoisomers appeared to negatively affect both germination and root growth at doses 5-10 times lower than those of (2S)-1-cyano-2-hydroxy-3-butene or (5R)-5-vinyl-1,3-oxazolidine-2-thione.     

Effect of tea catechins on regulation of antioxidant enzyme expression in H2O2-induced skeletal muscle cells of goat in vitro

Skeletal muscle cells (SMCs) of goats were stress induced with 1 mM H(2)O(2) in the absence or presence of 0.5, 5, and 50 μg/mL tea catechins (TCs) incubation. Cells were harvested at 48 h postincubation with TCs to investigate the effects of TCs on cell proliferation, cell membrane integrity, antioxidant enzyme activities, and antioxidant enzyme genes and protein expression levels. Results showed that H(2)O(2) induction inhibited cell proliferation with or without TC incubation; moreover, the inhibition effect was enhanced in the presence of TCs (P < 0.001). H(2)O(2)-induced stress increased the lactate dehydrogenase (LDH) activity in the absence or presence of TC incubation, but concentrations of TCs, less than 5 μg/mL, showed protective functions against LDH leakage than in other H(2)O(2)-induced treatments. The catalase (CAT) activity increased when SMCs were stress induced with H(2)O(2) in the absence or presence of TC incubation (P < 0.001). H(2)O(2)-induced stress decreased CuZn superoxide dismutase (CuZn-SOD) and glutathione peroxidase (GPx) activities, whereas this effect was prevented by incubation with TCs in a concentration-dependent manner. H(2)O(2)-induced stress with or without TC incubation had significant effects on mRNA and protein expression levels of CAT, CuZn-SOD, and GPx (P < 0.001). CAT and CuZn-SOD mRNA expression levels were increased by different concentrations of TC incubation, and this tendency was basically consistent with corresponding protein expression levels. The GPx mRNA expression level increased with a low concentration of TCs but decreased with concentrations greater than 5 μg/mL of TCs, whereas GPx protein expression in all TC-incubated groups was lower than in the control treatment. The current findings imply that TCs had an inhibitory effect on cell proliferation and enhanced damage to the cell membrane integrity, but TCs affected antioxidant status in SMCs by modulating antioxidant enzyme activities at mRNA and protein expression levels.