有益微生物的应用对海水对虾养殖池塘中细菌数量动态变化的影响

2001年5~7月,在深圳东海岸水产公司南澳半封闭式斑节对虾Penaeus monodom精养基地,进行了有益微生物的应用对虾塘总异养细菌和弧菌数量影响的调查研究。结果表明,对虾养成过程中,施用有益微生物的实验组虾塘水体中总异养细菌和弧菌数量明显较未施用的对照组虾塘低,但各虾塘水体中总异养细菌和弧菌数量的变化特征基本相同,即在养殖前期,虾塘水体中总异养细菌和弧菌数量均较稳定,并处于相对较低的水平;而在养殖中、后期,总异养细菌和弧菌数量均急剧升高,尤以对照组虾塘的升幅最大,其弧菌数量甚至超过100×102CFU.mL-1的对虾发病之弧菌数量临界值。实验组与对照组虾塘中表层沉积物中,总异养细菌和弧菌数量差异不大,总异养细菌和弧菌数量变化特征也基本相同,但实验组与对照组之间有所差异,其中实验组表现为双峰型的变化特征,峰值出现在养殖前期或中期和养殖将结束时;对照组则呈单峰型变化,峰值均出现在养殖中期。有益微生物的应用对虾塘水体中总异养细菌和弧菌数量的抑制效果较对沉积环境中的效果明显。     

海洋光合细菌筛选及其对养殖水体修复效果的测定

集约化投饵型养殖会导致养殖区域底部环境持续恶化,限制了水产养殖的可持续发展。对采自威海金海滩排污口附近污泥中的海洋光合细菌进行了富集、分离和初步鉴定,模拟自然养殖环境,选取优势菌株进行池塘底泥处理试验(上层水体,下层底泥),分析比较两株菌对底泥的改良效果;试验共获得两株优势光合细菌PSB1和PSB2,分属于红假单胞菌属(Rhodopseudomonas)和小红卵菌属(Rhodovulum)。底泥处理试验结果显示:PSB1和PSB2都可以稳定水体pH,降解水中的硫化物、氨氮和化学耗氧量(COD Mn)。PSB1处理底泥最佳效果为:添加1‰的PSB1,水体中pH在6.98~7.33,底泥中COD Mn 、硫化物、氨氮和NO - 2-N降解率分别为92.91%、79.61%、97.00%和73.56%。研究表明,光合细菌可以促进改善水质、维持下层水体的良好水质,在水产养殖中具有良好的应用前景。     

Comparison of four antibiotics with indigenous marine Bacillus spp. in controlling pathogenic bacteria from shrimp and Artemia

Use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. This has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. This study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. Ten pathogenic bacterial species isolated from shrimp, Penaeus monodon, and Artemia cysts were tested for susceptibility to indigenous marine Bacillus subtilis AB65, Bacillus pumilus AB58, Bacillus licheniformis AB69 and compared with oxytetracycline, chloramphenicol, gentamicin and bacitracin, which are common antibiotics used in Asian aquaculture. The Bacillus spp. were isolated from the local marine environment for bioremediation use in shrimp hatcheries and were proven to reduce total ammonium nitrogen. The pathogenic bacterial isolates were 90% susceptible to B. subtilis AB65, 70% susceptible to B. pumilus AB58 and B. licheniformis AB69 and 100% susceptible to oxytetracycline, chloramphenicol and gentamicin but only 40% to bacitracin. Two representative isolates of the vibrio group, Vibrio alginolyticus VaM11 and Vibrio parahaemolyticus VpM1, when tested for competitive exclusion by a common broth method using the marine Bacillus spp., showed decreased viable counts from 10(8) to 10(2) cfu mL(-1). The results suggest that the action of the marine bacteria appears to be significant in protecting the host shrimp against pathogenic bacteria. In addition to the alternative use of antibiotics, the selected marine bacteria had additional bioremediation properties of reducing ammonia.     

Extracellular protease production of bacteriolytic bacteria isolated from marine environments

ABSTRACT:   Fourteen bacterial strains isolated from marine environments exhibited antagonistic action against a wide range of bacteria including Vibrio spp. A double layer agar method was used for preliminary screening to determine the relative degree of growth inhibition or bacteriolysis exhibited by the isolates. Most of the antagonistic isolates were found to be Gram-negative, motile rods and were oxidase positive, and oxidative in the oxidation and fermentation test, suggesting that they are belong to the genera Pseudomonas . The antagonistic isolates lyzed the dead cells of marine Gram-negative bacteria in both plate and liquid methods. Bacteriolytic and casein hydrolytic activities were observed in the culture supernatant of the isolates. Anion exchange column chromatography (Toyopearl DEAE-650 M) was used to purify the extracellular protease produced by an antagonistic strain A1-J25a. The active fractions of protease collected from the eluted solution also exhibited bacteriolytic activity.     

Rapid enumeration of bacteria grown on surimi-based products by flow cytometry

ABSTRACT: A rapid method to enumerate bacteria adhered on a surimi-based product (kamaboko) by flow cytometry (FCM) is described. To remove Escherichia coli cells from the surface of kamaboko, ultrasonic energy was used. Almost all cells can be removed from kamaboko in 3 min with ultrasonic treatment. Because the sample might contain various non-bacterial particles such as food additives and debris of products, propidium iodide was used to discriminate bacterial cells from non-bacterial particles. Fluorescence scattergrams could distinguish bacteria from the particles, and the FCM method could be used to enumerate bacteria adhered on the surface of kamaboko during storage. Cell numbers determined by FCM paralleled well with those measured using a traditional colony counting method in the range of 10⁴–10⁸ cells/g. The FCM assay could enumerate cells within 1 min and the total assay time, including sample preparation, was less than 30 min.     

添加不同菌剂海水水族箱硝化功能建立过程的比较研究

分别选取自制硝化细菌、德彩、喜瑞和科迪4种硝化细菌制剂,研究其对海水水族箱硝化功能建立过程的影响。结果表明:添加不同硝化细菌海水水族箱硝化功能建立过程明显不同,自制硝化细菌制剂组海水水族箱氨氮在5 d达到峰值,11 d降至最低;德彩、喜瑞和科迪组则分别在15 d、13 d和17 d达到峰值,30 d、36 d和30 d降至最低;自制硝化细菌制剂组海水水族箱中亚硝酸盐在21 d达到峰值,在40 d降低至检测不出;德彩硝化细菌、喜瑞硝化细菌和科迪硝化细菌组亚硝酸盐分别在46 d、59 d、68 d到达峰值,德彩、喜瑞组亚硝酸盐分别在69 d、75 d降至检测不出,而科迪组亚硝酸盐直至实验结束仍维持在较高浓度(80 mg/L)。     

Use of marine yeasts as an available diet for mass cultures of Moina macrocopa

A 4‐week feeding trial was conducted to test the use of marine yeasts purified from seawater and sediments as a dietary source in cultivating a cladoceran, Moina macrocopa, a potential alternative live food for fish larvae. Optimal growth conditions of two yeast strains were obtained for NaCl concentration, pH and temperature. Moina macrocopa was cultivated using different diets: marine yeasts (Debaryomyces hansenii Yeast‐14 and Candida austromarina Yeast‐16) and a commercial diet (Erythrobacter sp. Sπ‐I). The essential amino acids of both the yeast strains were nearly as great as those in M. microcopa. Further, the yeast‐fed M. macrocopa had essential amino acid profiles similar to the documented values for rotifers and Artemia enriched in microalgae and commercial diets. Erythrobacter sp. Sπ‐I lacked n‐3 polyunsaturated fatty acids, 20:5n‐3 and 22:6n‐3, which were also low but detected in both yeasts. An increase in the 20:5n‐3 [eicosapentaenoic acid (EPA)] and 22:6n‐3 [docosahexaenoic acid (DHA)] levels, compared with the levels in yeast strains themselves, was more pronounced in the 22:6n‐3 level of Moina fed the C. austromarina, resulting in a high DHA:EPA ratio. When the Moina diets were switched, their δ¹³C values shifted gradually towards the values of the switched diets. Diet switch from Erythrobacter sp. Sπ‐I to C. austromarina Yeast‐16 resulted in a more rapid turnover of Moina tissue carbon than that in the inverse case. When fed a mixed diet, the δ¹³C values of Moina tissue approached the value of marine yeasts immediately. These temporal changes in the δ¹³C values of Moina tissue indicate the preferential ingestion of marine yeasts and a selective assimilation of the carbon originated from marine yeasts. These findings suggest that marine yeasts, particularly C. austromarina Yeast‐16, are highly available to mass cultures of M. macrocopa, providing better nutritional and dietary values than the commercial diet (Erythrobacter sp. Sπ‐I).     

水下集鱼灯在近海水体中的光场分布数值模拟

为提高水下集鱼灯光场分布计算精度,本实验采用集鱼灯光谱数据、光度分布数据和海水固有光学参数,基于蒙特卡罗模拟方法构建了新的光场传输数值模拟方法,将光束能量离散成大量光子,通过追踪光子路径计算水下集鱼灯形成的光场强度,并开展水槽实验对计算方法进行了验证。同时,提出了新的集鱼灯光场分布评价指标I_ds和A_ds。利用模型分析了海水的叶绿素a质量浓度、散射作用类型和散射相位函数HG (Henyey Greenstein)中非对称参量g的取值对水下光场分布的影响。结果显示,①叶绿素a质量浓度从1mg/m³增加到5 mg/m³,LED集鱼灯A_5m减少了72.73%,MH集鱼灯A_5m减少了72.17%;LED集鱼灯I_5m减少了66.69%,MH集鱼灯I_5m减少了53.29%。②当海水介质引起的散射类型分别为米氏散射和瑞利散射时,MH集鱼灯I_5m分别为1 058.48和1 020.5 lx,LED集鱼灯I     

大鹏澳网箱养殖区沉积物硫酸盐还原菌（SRB）及其与环境因子关系的初步研究

实验采用MPN法测定了大鹏澳网箱养殖区沉积物中硫酸盐还原菌(SRB)的含量,布设的5个站位SRB检出率达100%,SRB平面分布特征是鱼类网箱区>对照区>浮筏贝类区;垂直分布特征为1cm层>5cm层>10cm层,SRB数量是由上向下逐渐减少。探讨了SRB含量与相应沉积物样品中的硫化物含量、氧化还原电位、pH等环境因子的关系。结果表明,沉积物中SRB数量与硫化物含量呈显著性正相关(R=0.96,P<0.05),与氧化还原电位(OPR)呈极显著性负相关(R=-0.97,P<0.01),与pH之间不具有显著性关系。     

海洋渔业电子地图系统软件设计与实现

系统地介绍了海洋渔业电子地图系统软件EA2000的数据模型和主要功能的设计与实现，EA2000采用了面向对象的数据模型，紧密的矢栅数据接口，以及图层－地图－图集一体化数据组织管理方法，该软件具有开放的数据输入接口，图层创作的自动化和动态交互式编辑，双向查询检索，缩放漫游与多级比例尺联动显示，动态显示，对象信息自动显示，地理信息系统渔船动态监控管理，多媒体信息插入管理，互操作等功能。     

海水网箱远程投饵测控系统的设计

为使养殖户在岸上就能对海水网箱的投饵进行实时控制、修改投饵设置和查询网箱有关信息,尤其是当投饵发生异常时,能即时收到告警信息,以保证正常自动投饵,设计了一种基于GSM Modem的海水网箱远程投饵测控系统。海上投饵装置由桨叶式投饵器为核心的机械和测控两部分组成。测控部分硬件以STC5410AD单片机为核心,由通信、信息采集、实时时钟和输出驱动等模块组成,文中着重介绍了系统的软硬件设计。运行结果证明,设计完全达到了预定要求,工作稳定可靠,操作简便。     

海洋渔业地理信息系统的发展、应用与前景

渔业科学多年来一直注重研究生物形态学、分类学、行为学、生理学、细胞学和育种学等 ,而对于鱼类种群或群聚及其组成成分在海洋中的分布格局、形成、演变及其与环境条件的关系或驱动过程等空间方面的研究较少[1] ;另一方面 ,地理信息系统 (Ｇｅｏｇｒａｐｈｉｃｉｎｆｏｒｍａｔｉｏｎｓｙｓｔｅｍ ,ＧＩＳ)作为处理空间问题的有力工具愈来愈被各学科所接受。上世纪 80年代中后期ＧＩＳ开始应用于海洋渔业研究[2 ] ,并在 90年代得到了较大的发展[3 ] 。海洋渔业地理信息系统的发展可以分为三个阶段 :海洋渔业制图 ,海洋渔业电子图集 ,海洋…     

关于改善我国海洋渔业统计制度的建议

可靠的渔业统计制度是渔业管理决策最重要的基础支撑,也是渔业监督管理的重要依据和手段。文章扼要地介绍了《联合国海洋法公约》等国际条约和文件对渔业统计的基本要求、有关国家和我国的渔业统计制度,指出了我国层层上报的全面渔业统计方式已不能适应当前渔业经济体制和管理需求、渔业统计指标体系不完整、难以全面反映渔业实际、统计监督和数据核实制度存在不足、渔业统计数据信息化滞后等问题。建议:(1)严格执行《渔业法》明确规定的捕捞日志制度;(2)规定渔获物只能在限定港口卸载,建立渔获物上报与统计监督制度;(3)逐步实行随船观察员制度;(4)完善立法,提供法律支撑和保障。     

不同基质构建海水养殖系统硝化功能的比较分析

为比较不同基质构建海水养殖系统硝化功能的强弱,选取纤维毛球、陶粒、螺旋式生物绳等7种基质,其中陶粒、流化床填料、纤维毛球采取不同放置方式,共建立14个模拟海水养殖系统,比较不同基质硝化功能建立过程以及同种基质不同放置方式对氨氮和亚硝氮的去除效果。结果表明,单位体积珊瑚骨的氨氧化活性和亚硝酸盐氧化活性高于其他载体,在氨氮初始浓度20 mg/L条件下,氨氮和亚硝氮降解至检测不出分别需要3 d和11 d,而纤维毛球、陶粒、螺旋式生物绳、流化床填料、丝带内芯悬浮球、海绵内芯悬浮球硝化系统的建立分别需要18、26、30、25、27和22 d。纤维毛球100目筛绢悬挂、陶粒网兜悬挂、流化床填料100目筛绢悬挂优于其他放置方式,其中纤维毛球100目筛绢悬挂硝化功能建立时间为18 d,效果最优,流化床填料100目筛绢悬挂、陶粒网兜悬挂硝化系统建立分别需要21、24 d。     

Use of focal species in marine conservation and management: a review and critique

1. Focal species (i.e. indicators, keystones, umbrellas, and flagships) have been advocated for the management and conservation of natural environments. 2. The assumption has been that the presence or abundance of a focal species is a means to understanding the composition and/or state of the more complex community. 3. We review the characteristics of focal species, and evaluate their appropriateness and utility judged against conservation objectives. 4. It appears that indicator species (of both composition and condition) may be of greatest general utility, and that several types of focal species may exhibit useful indicator properties. Copyright © 2001 John Wiley & Sons, Ltd.     

Reef quality criteria for marine reserve selection: an example from eastern Brazil

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A novel combined recirculating treatment system for intensive marine aquaculture

This study established a pilot‐scale recirculating treatment system that coupled an ecological process with a biological process to achieve adequate water quality and to minimize the water consumption for intensive marine culture. The recirculating treatment system consisted of a settling cell, a biofilter tank, a bivalve tank and gravel beds. The toxic pollutants, threatening the growth of bivalves, were reduced by the settling cell and the biofilter tank, so that the polyculture of shrimp and bivalves could be achieved. The living bivalve tank could function well as a remover of remaining small suspended solids (SS), and other pollutants. As the SS was reduced to a very low level by bivalve tank before the water flowing into the gravel beds, the risk of clogging was prevented. The studies suggested that the system maintained high removal efficiencies of SS, ammonium nitrogen () and nitrite nitrogen () and could contribute to the increase in shrimp yield.     

外来海洋物种入侵风险评估体系的构建

为了评估外来海洋物种入侵的风险,根据外来海洋物种入侵特点,在文献分析和专家咨询的基础上,采用层次分析和三角模糊数数值标度计算权重的方法,设计5个一级指标和20个二级指标,构建了外来海洋物种入侵风险评估体系。利用该评估体系对7种（类）典型海洋外来种进行了风险评估。评估结果表明,互花米草和对虾白斑病毒为极高风险等级;沙筛贝、米氏凯伦藻和帕金虫为高风险等级,罗非鱼为中风险等级;大菱鲆为低风险等级。由于传统层次分析法的“1～9标度”方法不能精确地反映人的实际思维,从而影响最终的判定结果,本评估体系借鉴了三角模糊数表示的改进方法来计算权重值,并对一级指标和二级指标的相对权重均进行了权重设计,得出的结果更加精确地反映了人的实际思维。外来海洋物种入侵风险评估体系的建立将为外来海洋物种入侵风险管理提供决策依据和参考。