Mangrove structure,litter and macroalgal productivity in a northern‐most forest of Florida

Rhizophora mangle L. dominated 10 overwash islands within Tampa Bay forming the northernmost mangrove forests on the west coast of Florida. The mean number of trees and basal area were 5040 trees ha^-1 and 20.5m²ha^-1, respectively. Basal areas ranged from 1.1 (Avicennia germinans (L.) Stern), to 2.6m²ha^-1 (Laguncularia racemosa Gaertner), to 16.8 (R. mangle). Cockroach Bay mangroves are small (5.8–7.0m tall) versus coastal forests of south Florida and the Caribbean. Total litter production for a 12 month period was maximal in September for fringing (7.4gdwtm^-2d^-1) and interior (8.7gdwtm^-2d^-1) areas with the two zones not being significantly different. Average litter fall (3.1gdwtm^-2d^-1) was similar to more tropical Caribbean mangals. Leaves accounted for 68 of the litter and reproductive material for 6, being similar to tropical riverine and overwash marine angiosperm communities on the Mexican coast. Although mangrove forests in Tampa Bay are small in stature and experience cold damage and occasional frosts, their litter fall is similar in biomass to that of more tropical mangals. However, their reproductive output is low based on litter fall versus more tropical mangals suggesting that the northern extension is less than optimum. Macroalgal diversity was low (10 species) as compared to Caribbean mangals. Macroalgal turf (0.8gCm^-2d^-1) and epiphyte communities of A.germinans pneumatophores (2.7gCm^-2d^-1) have productivity levels that are equal to or greater than those of Caribbean mangals.     

Sediment accretion and variability of sedimentological characteristics of a tropical estuarine mangrove: Kemaman, Terengganu, Malaysia

This paper reports on a study of accretion rate and sedimentological variability in a estuarine mangrove swamp. One hundred and sixteen stations were monitored for 2 years. In addition, surface sediment samples were collected at 52 stations during the months of May, June and July (1994) to represent the nonmonsoon sediments and November, December (1994) and January (1995) to represent the monsoon sediments. Results show that the accretion rate for the first year was 1.46±0.13cm/yr and 0.66±0.04cm/yr for the second year thus making the average accretion for the 2 years period to be 1.06cm/yr. The average accretion rate for the monsoon season (0.26 ± 0.04cm/month) was found to be significantly higher than the nonmonsoon season (0.12 ± 0.03cm/month). Nevertheless, the same is not true for the surface sediment characteristics. The sedimentological characteristics between the monsoon and the nonmonsoon sediments were not significantly different.     

Estimated stocks of organic carbon in mangrove roots and sediments in Hinchinbrook Channel,Australia

Aboveground and belowground root biomasses (Babove and Broot) were measured for young, isolated Rhizophorastylosa on Iriomote Island, Japan. The relationship between these two parameters was significant and given as the equation, Broot(g dry weight) = 0.394 × Babove(g dry weight) – 485 (r = 0.986). Multiple regression analyses also revealed good correlation between diameter and biomass of prop roots (Dprop and Bprop) and between prop root and root biomasses. Consequently, root biomass could be estimated from the measurements of diameter and biomass of prop roots using the multiple regression equation, Broot(g dry weight) = 80.0 ×Dprop(cm) + 0.86 ×Bprop (g dry weight) – 251. The relationship between DBH (diameter at breast height) and prop root biomass was also adequately described using an allometric equation.In Hinchinbrook Channel, Australia, redox potential (measured as Eh) and organic carbon stocks in the top 5cm of mangrove sediments were measured along a 600m transect from the frequently inundated, Rhizophora dominated zone on the creek edge, towards higher grounds, where Ceriops spp. became increasingly dominant. Eh values were about –60mV near the creek edge and increased to 260mV on higher grounds. Organic carbon stocks showed an opposite trend to Eh, with the values decreasing from about 360tCha–1 to 160tCha–1. At 18 sites, representing six different habitats, organic carbon stocks were also measured along with the DBH of mangrove trees. DBH was converted into aboveground biomass and then into root biomass using the equations obtained in the study on Iriomote Island. The average organic carbon stocks in the top 50 cm of sediments, aboveground biomass and root biomass were 296tCha–1, 123 tCha–1 and 52 tCha–1, respectively, and accounted for 64%, 25% and 11% of the total organic carbon stock.     

Water circulation, groundwater outflow and nutrient dynamics in Mida creek, Kenya

The relationship between physical hydrodynamic processes and nutrients dynamics was investigated in Mida creek, a groundwater influenced mangrovefringed creek in Kenya between March 1996 and May 1997. The research involved spot and timeseries measurement of nitrate–nitrite, ammonia, silicates, phosphates, salinity, temperature, sealevel as well as tidal currents at seven stations located in the front, middle and backwater zones of the creek. Groundwater level as well as total dissolved solids' concentration, salinity, temperature and nutrients' concentration were also measured once every month in shallow wells (watertable<5m) located in the upper region of the creek. Results of the study show that nutrient concentrations vary with the tide and that, though there is no river drainage, they are of the same magnitude as in mangrove creeks with substantial river runoff. The peak concentrations of NH ₄ ⁺ –N (5.45M), NO ₂ –NO ₃ (5.63M), PO ₄ ^3– –P (0.58M) and SiO ₃ ^2– –Si (81.36M) in the creek occurred during flood tide, 2–3h before high waters. The (NO ₂ ^– + NO ₃ ^– )–N concentrations declined rapidly during ebb tide, reaching the minimum levels during low water. Contribution of groundwater seepage to the net nutrients flux (particularly on nitrite–nitrates) is largest in dry seasons. The study shows that groundwater outflow sustains the mangroves during periods of severe salinity stress and nutrients deficiency in dry seasons. This is essentially by limiting salinity increase and by boosting nutrient supply in dry seasons.     

Sources,sinks, and export of organic carbon through a tropical,semi‐enclosed delta (Hinchinbrook Channel,Australia)

A mass balance for organic carbon in Hinchinbrook Channel was constructed to identify major sources, sinks, and the magnitude of organic matter available for export to the adjacent coastal zone. Total organic carbon input from the Herbert River and from net production of mangroves, phytoplankton, seagrasses, and benthic microalgae is 8.94 ×109M Corgyr–1 (moles organic carbon per year). Mangroves and river inputs are the largest carbon sources, accounting for 56% and 27% of the total annual input, respectively. Benthic respiration and burial in sediments are the major sinks, accounting for 46% and 41% respectively of total losses (3.09 ×109 M Corgyr–1). This mangrovedominated coastal ecosystem is net autotrophic, with 5.85×109M Corgyr–1 (65% of total Corg input) available for export to the adjacent nearshore zone. Total export of organic carbon from the region (adding carbon export from Missionary Bay mangroves on the northern end of Hinchinbrook Island) amounts to 82,800 metric tons of organic carbon per year. These results confirm earlier evidence indicating that much of the particulate sediment carbon in the adjacent coastal zone is of mangrove origin. This mass balance, although preliminary, demonstrates the importance of Hinchinbrook Channel as a source of organic matter for the Great Barrier Reef lagoon.     

Concentration and molecular weight distribution of dissolved organic carbon in a mangrove creek in the Hinchinbrook area,Australia

The concentration and molecular weight distribution of dissolved organic carbon (DOC) are reported for a mangrove creek in the Hinchinbrook area, Australia. DOC concentration ranged from 1.0mg Cl–1 near the creek mouth to 2.2mg Cl–1 at the innermost part of the creek. There was no apparent spatial trend in molecular weight distribution of DOC, with a >300gmol–1 fraction accounting for about 70% of the total in all samples. DOC concentration fluctuated between 0.5 and 1.2mg Cl–1 over one tidal cycle. The samples collected at low tide and during the rising tide were dominated by DOC of >300gmol–1 and <300gmol–1, respectively. This suggested the export of high molecular weight DOC, probably originating from litter leachates, and the import of low molecular weight DOC to the creek.     

Gamma radiation sensitivity of larvae and adults of the red flour beetle, <Emphasis Type="Italic">Tribolium castaneum</Emphasis> Herbst

In this study, the susceptibility of larvae and adults of Tribolium castaneum (Herbst) to gamma radiation was investigated in the laboratory in Turkey. Gamma radiation was applied at 6 dose levels between 20 and 200Gy to 13–15day-old adults and at 5 dose levels between 20 and 180Gy to 18–20day-old larvae of T. castaneum. All experiments were performed in growth chambers maintained at 27±1°C and 70±5% r.h. The larvae proved to be the more susceptible stage, survival to the adult stage being prevented by exposure of the eggs to 100Gy; the adult stage was less susceptible. LD₅₀ and LD₉₉ values were determined as 19,75 and 42,97Gy for larvae and 33,21 and 64,50Gy for adult stage, respectively. It was concluded that 100Gy is the effective dose for both larval and adult stages.     

Effects of nitrogen and phosphorus fertilization on the growth and nutrition of hybrid poplars on Vancouver Island

The effects of nitrogen (N) and phosphorus (P) rates, P source, and method of P application were tested on growth and nutrition of four clones of hybrid poplar (Populus trichocarpa Torr. & Gray×P. deltoides Bartr. ex Marsh or P. trichocarpa×P. maximowiczii A. Henry) in a plantation on Vancouver Island. Treatments were applied shortly after planting. Nitrogen (0, 500kgNha^–1) was supplied as ammonium sulfate (AS); half of the AS was added at the start of the second growing season. Phosphorus (0, 100, 200kgPha^–1) was supplied as triple super phosphate (TSP), rock phosphate (RP), or diammonium phosphate (DAP) and banded or broadcast. Over four growing seasons, stem volumes increased with AS and P additions. The effects of AS and P each were greatest when the other was also added. Volume was greater when P was applied at 100kgha^–1 than in the P control and did not significantly increase further at 200kgPha^–1. Phosphorus additions were more effective when added as TSP or DAP than when added as RP, but only in P100 and when banded. Banding increased volume when P was applied at 200kgha^–1, but increases were significant only for DAP fertilized trees. Roto-tilling associated with the banding treatment also increased volume when AS and P were not added. Rankings of clones with respect to stem volume varied with N supply and changed over time.     

In vitro propagation of <Emphasis Type="Italic">Oroxylum indicum</Emphasis> Vent. a medicinally important forest tree

In vitro propagation of Oroxylum indicum Vent. was carried out using cotyledonary node explants. Among the different types of cytokinins used for culture establishment, 6-benzyladenine exhibited the best response with higher concentrations (8.87µM or above) for inducing multiple shoots. Inclusion of indole-3-acetic acid (2.85µM) into 6-benzyladenine-supplemented medium triggered a high frequency of response as well as a proliferation of shoots. The best medium for proliferation was Murashige-Skoog (MS) medium with 6-benzyladenine (8.87µM) and indole-3-acetic acid (2.85µM). However, incorporation of gibberellic acid (1.44µM) was mandatory to enhance shoot elongation. Repeated subculturing of cotyledonary node and in vitro developed nodal segments in MS medium with 6-benzyladenine (4.44µM) at 4-week intervals resulted in continuous mass multiplication of shoots without any evidence of decline. Root induction was best (91.6%) when MS strength was reduced to one-quarter and combined with -naphthalene acetic acid (2.69µM) and indole-3-acetic acid (5.71µM), with a high survival rate (70–72%) of plantlets hardened in either soil rite or soil:sand:soil rite (1:1:2).     

Effects of a nursery CO2 enriched atmosphere on the germination and seedling morphology of two Mediterranean oaks with contrasting leaf habit

The use of an enriched CO₂ atmosphere in tree nurseries has been envisaged as a promising technique to increase productivity and to obtain seedlings with a higher root/shoot ratio, an essential trait to respond to water stress in Mediterranean-type ecosystems. In that framework, we have analyzed the effects of three levels of atmospheric CO₂ concentration (350, 500 and 700ppm) on the germination rate, growth and morphology of seedlings of two Mediterranean oaks used in reforestation programs: the evergreen Quercus ilex L. and the deciduous Quercus cerrioides Wilk. et Costa. CO₂ enrichment increased the germination rate of Q. cerrioides (from 70±7 to 81±3%) while it decreased that of Q. ilex (from 71±10 to 41±12%). Seedlings of both species increased approximately 60% their total biomass in response to CO₂ enrichment but at two different CO₂ concentrations: 500ppm for Q. cerrioides and 700ppm for Q. ilex. This increase in seedlings biomass was entirely due to an augmentation of root biomass. Considering germination and biomass partitioning, an enriched CO₂ atmosphere might not be appropriate for growing Mediterranean evergreen oaks, such as Q. ilex, since it reduces acorn germination and the only gains in root biomass occur at a high concentration (700ppm). On the other hand, a moderate CO₂ enrichment (500ppm) appears as a promising nursery technique to stimulate the germination, growth and root/shoot ratio of deciduous oaks, such as Q. cerrioides.     

Effects of soil solarization on nematodes in Croatia

For the first time soil solarization was investigated in Croatia both in the field and in the greenhouse in 1991, 1992, 1993 and 1994. For two months (July and August), the soil was mulched with transparent polyethylene (PE) sheets of 0.015 or 0.050mm thickness. Soil temperatures at depths of 5, 10 and 20cm were recorded daily. In order to assess nematode population densities, soil samples were analysed before mulching and at the end of the mulching treatment. The results of these experiments showed that soil solarization drastically reduced the population of plant-parasitic nematodes (Meloidogyne, Pratylenchus, Paratylenchus, Tylenchus, Tylenchorhynchus spp.) by about 97–100% at a depth of 10cm and 92–97% at a depth of 20cm in the field, while in the greenhouse, the population of plant-parasitic nematodes was reduced by about 89–100% at a depth of 10cm and 98–100% at a depth of 20cm.In the same experiments, the population of saprophytic nematodes in the field was reduced by about 86–90% at a depth of 10cm and 72–89% at a depth of 20cm. In the greenhouse, the population of saprophytic nematodes was reduced by about 87–97% at a depth of 10cm and 87–93% at a depth of 20cm. This data shows that soil solarization was less effective in the control of saprophytic nematodes, which is considered to be an advantage.     

Biological performance of wood-based composites treated with a formulation of 3-iodo-2-propynyl butylcarbamate and silafluofen using supercritical carbon dioxide

Wood-based composites (medium density fiberboard, hardwood plywood, softwood plywood, particleboard, and oriented strand board) treated with a mixture formulation of 3-iodo-2-propynyl butylcarbamate (IPBC) and silafluofen using supercritical carbon dioxide as a carrier solvent were evaluated for their resistance to biological attack in a laboratory study. The formulation was pre-pared by mixing 10g of IPBC and 1g of silafluofen in ethanol solution (20ml). Treatments were conducted at 35°C/7.85MPa, 35°C/9.81MPa, and 55°C/11.77MPa with the direct introduction of 20ml of the formulation into the treatment vessel with a capacity of ca. 2000ml at a rate of 2ml/min. Laboratory tests indicated that the treatment conditions used significantly enhanced the resistance of the treated wood-based composites against fungal and termite attacks. Because no significant difference in efficacy against both biodegrading agents was noticed regardless of the treatment conditions, the treatment at 35°C/7.85MPa was thought to be the most economical in terms of energy consumption and performance of treated materials. However, the amount of biocides in a formulation must be carefully selected in accordance with the required treatment condition to ensure satisfactory performance of the treated wood-based composites against any biological agent.     

Compressive deformation of wood impregnated with low molecular weight phenol formaldehyde (PF) resin I: effects of pressing pressure and pressure holding

The deformation behavior of low molecular weight phenol formaldehyde (PF) resin-impregnated wood under compression in the radial direction was investigated for obtaining high-strength wood at low pressing pressures. Flat-sawn grain Japanese cedar (Cryptomeria japonica) blocks with a density of 0.34g/cm³ were treated with aqueous solution of 20% low molecular weight PF resin resulting in weight gain of 60.8%. Oven-dried specimens were compressed using hot plates fixed to a testing machine. The temperature was 150°C and the pressing speed was 5mm/min. The impregnation of PF resin caused significant softening of the cell walls resulting in collapse at low pressures. The cell wall collapse was strain-dependent and occurred at a strain of 0.05–0.06mm/mm regardless of whether the wood was treated with PF resin. Thus, pressure holding causing creep deformation of the cell walls was also effective in initiating cell wall collapse at low pressure. Utilizing a combination of low molecular weight PF resin impregnation and pressure holding at 2MPa resulted in a density increase of PF resin-treated wood from 0.45 to 1.1g/cm³. At the same time, the Youngs modulus and bending strength increased from 10GPa to 22GPa and 80MPa to 250MPa, respectively. It can be concluded that effective utilization of the collapse region of the cell wall is a desirable method for obtaining high-strength PF resin-impregnated wood at low pressing pressures.     

Preservative treatment of wood-based composites with 3-iodo-2-propynyl butylcarbamate using supercritical carbon dioxide impregnation

Five structural-use wood-based composites [medium density fiberboard (MDF), hardwood plywood, softwood plywood, particleboard, oriented strand board (OSB)] were treated with 3-iodo-2-propynyl butylcarbamate (IPBC) using supercritical carbon dioxide (SC-CO₂) as a carrier solvent. Treatment was conducted at 35°C/7.85MPa (80kgf/cm²), 35°C/9.81MPa (100kgf/cm²), 35°C/11.77MPa (120kgf/cm²), 45°C/7.85MPa, 45°C/9.81MPa, 45°C/11.77MPa, 55°C/7.85MPa, 55°C/9.81MPa, and 55°C/11.77MPa. A decay test was carried out in the laboratory according to the modified Japanese standard method in which untreated and treated specimens were exposed for 12 weeks to a monoculture of the white-rot fungus Trametes versicolor (L.: Fr.) Pilat or the brown-rot fungus Fomitopsis palustris (Berk. et Curt.) Gilbn. and Ryv. IPBC/SC-CO₂ treatment protected the treated materials from decay by the two fungi, although the relative efficiency against decay varied with the treatment conditions and the type of wood-based composite. Better performance for MDF, hardwood plywood, and particleboard was generally obtained at high temperatures and pressures, whereas softwood plywood and OSB were most protected at low temperatures under any of the pressure levels tested.Part of this work was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002     

Effect of thawing regime on growth and mortality of frozen-stored Norway spruce container seedlings planted in cold and warm soil

One-year-old frozen-stored Norway spruce (Picea abies (L.) Karst.) container seedlings were planted in a controlled environment providing an air temperature of 22°C and soil temperature of 9±1 or 18±1°C. At planting the root plugs were either frozen or had been thawing for 4 days at 9°C. During a 5-week growing period, in both cold and warm soil the root growth and height growth were less in frozen-planted seedlings than in thawed seedlings. In addition, frozen-planting delayed bud burst and increased mortality. Soil temperature, however, had no effect on bud burst or mortality. Low soil temperature retarded root growth of seedlings thawed before planting but resulted in both retarded root growth and height growth if root plugs were frozen when planted. These results indicate that planting Norway spruce seedlings with frozen root plugs constitutes a considerable risk for successful forest regeneration at soil temperatures normally prevailing in Fennoscandia in spring or early summer especially if the soil is dry at the time of the planting.     

Canopy cover effects on nitrogen and phosphorus status in understory vegetation in oak and pine stands

The rate of change of leaf mass, N and P levels in understory vegetation at various levels of canopy cover were measured for 2 years following canopy cover manipulations in northern red oak (Quercus rubra L.) and red pine (Pinus resinosa Ait.) stands in northern Lower Michigan, USA. Canopy cover treatments consisted of clearcut, 25% (50% during first sampling year), 75%, and uncut. Leaf mass, and N and P contents were significantly higher in the clearcut treatment than in other canopy cover treatments, except for the 25% treatment in red pine stands. Leaf N concentrations in understory vegetation were also significantly higher in the clearcut (1991, 20.8mgg^–1; 1992, 22.4mgg^–1) than in the uncut treatment (1991, 16.5mgg^–1; 1992, 16.9mgg^–1). Canopy type (northern red oak and red pine) had little influence on understory nutrient status and leaf mass. In addition, fronds of bracken ferns in all canopy cover treatments in both northern red oak and pine stands were a major sink of nutrients in the understory. The results of this study showed that partial canopy removal generally had only a minor impact on understory leaf production and nutrient status compared with clearcuts during the 2-year period following canopy removal.     

Manufacture and properties of low-density binderless particleboard from kenaf core

Low-density binderless particleboards from kenaf core were successfully developed using steam injection pressing. The target board density ranged from 0.10 to 0.30g/cm³, the steam pressure used was 1.0MPa, and the steam treatment times were 7 and 10min. The mechanical properties, dimensional stability, and thermal and sound insulation performances of the boards were investigated. The results showed that the low-density kenaf binderless particleboards had good mechanical properties and dimensional stability relative to their low board densities. The board of 0.20g/cm³ density with a 10-min treatment time produced the following values: modulus of rupture 1.1MPa, modulus of elasticity 0.3GPa, internal bond strength 0.10MPa, thickness swelling in 24h water immersion 6.6%, and water absorption 355%. The thermal conductivity of the low-density kenaf binderless particleboards showed values similar to those of insulation material (i.e., rock wool), and the sound absorption coefficient was high. In addition, the boards are free from formaldehyde emission. Kenaf core appears to be a potential raw material for low-density binderless panels suitable for sound absorption and thermally resistant interior products.Part of this report was presented at the 52th Annual Meeting of the Japan Wood Research Society, Gifu, Japan, April 2002     

Field and model studies of the fate of particulate carbon in mangrove‐fringed Hinchinbrook Channel,Australia

A field and model study was undertaken in 1996/1997 of the dynamics of water, fine sediment and particulate carbon in the northern region of the mangrovefringed Hinchinbrook Channel, Australia. The currents were primarily tidal and modulated by the wind. Biological detritus acted as a coagulant for the fine cohesive sediment in suspension in the mangrovefringed, muddy coastal waters. Plankton and bacteria were the major aggregating agents at neap tides, and mangrove detritus at spring tides. The microaggregates were typically several hundreds of micrometer in diameter and enhanced the settling rate. The fate of fine sediment and particulate carbon was controlled by the dynamics of the coastal boundary layer, a turbid shallow coastal water zone along the mangrovefringed coast. A tidallymodulated, turbidity maximum zone was found in this layer. Wind stirring increased the turbidity by a factor of five.The channel behaves as a sink trapping fine sediment and particulate carbon. However, the sink was leaky because the dynamics of the coastal boundary layer generated a net outflow of fine sediment out of the channel along the western coast. The biologically enhanced settling of cohesive sediment limited the offshore extent of the muddy suspension to within a few hundreds of meters from the coast.At spring flood tides, some of this particulate carbon was advected into the mangrove forest where it would remain trapped. On a yearly basis about six times as much particulate carbon was exported out of Hinchinbrook Channel through the coastal boundary layer than was trapped in the fringing mangroves.     

Analysis of current forest operations in the Okumikawa Forestry Area by location and topography

We analyzed current forest operations in Toei, in the Okumikawa Forestry Area, Japan, by examining actual achievement, location, and topography. We considered five basic forest operations conducted by the Forest Owners Association between 1991 and 2000 in a subsidized reforestation and silviculture project: planting, weeding, pruning, cleaning, and thinning. In addition, we considered the road network, including public, forest, and strip roads. The area in which forest operations have been conducted over the past 10 years amounted to less than two-thirds of the area targeted in the Toei Forest Improvement Plan. The mean access distance of the work sites was 164m, which was 52m less than the 10-year average for the whole forest (216m). Forest operations tended to ignore remote forest areas where the topography was steep, due to cost, the logging techniques required, and labor requirements. We determined that the limitations on forest operations in Toei were a 300m access distance, an elevation of 800masl, and a 90% slope. In accessing work sites, the utilization ratios of forest and strip roads were higher than their composition ratios, and public roads were used most. Newly constructed strip roads were used more than forest roads. Based on current forest operations, we calculated the road density and length required to be 7.1m/ha and 79.2km, respectively, with an estimated cost exceeding 720 million yen for new construction.     

Estimation of the biomass of fine roots and mycorrhizal fungi: a case study in a Japanese red pine (Pinus densiflora) stand

As part of a study on soil carbon flow in forest ecosystems, the biomass of fine roots (2.0mm in diameter) and root-associated fungi, including ectomycorrhizal fungi, were estimated in the summer season in 1998 at a Pinus densiflora (Japanese red pine) stand in western Japan. Fine roots of pine were classified into three categories: class I roots (0.5–2.0mm in diameter), long class II roots (long roots with diameter 0.5mm; II_L), and short class II roots (short roots with diameter 0.5mm; II_S). Total biomass of fine roots (I + II_L + II_S) at this stand was estimated to be 91.0gm^–2, about 23% of which was class II roots (II_L + II_S). Ergosterol, which is a component of fungal membranes, was analyzed to estimate the biomass of root-associated fungi in roots. In the upper soil layers (from the surface to 13.4cm in depth), ergosterol contents in the class I, II_L and II_S roots were in the ranges 43.1–82.2, 126.1–196.3 and 271.2–321.0µgg^–1 root DW, respectively. The ergosterol content was converted to fungal biomass using the median (minimum–maximum) value of ergosterol concentration reported for ectomycorrhizal fungi. Root-associated fungal biomass in this stand was estimated to be 2.0 (0.5–9.6) gm^–2. The data suggest the biomass of ectomycorrhizal fungi in the P. densiflora stand is small compared with that in other forest ecosystems.