Proteolysis and amino acid degradation during ensilage of untreated or formic acid-treated lucerne and maize

Lucerne ( Medicago sativa ), field wilted to 330 g dry matter (DM) kg⁻¹, and whole-plant maize ( Zea mays ), 383 g DM kg⁻¹, were treated with formic acid (FA) at the rate of 4-5 1 t⁻¹ fresh forage. The effects of FA treatment on proteolysis and amino acid degradation was investigated at several intervals over a 90-d period of ensilage in laboratory silos. Formic acid treatment produced different patterns of pH decline during ensilage of the two forages, suggesting some degree of crop specificity in response to FA treatment. After 7 d of ensilage of lucerne there were increases of 81% in nonprotein nitrogen (NPN), and 104% in free amino acid nitrogen (FAA-N), but with FA-treated lucerne the increases were 39% and 31%, respectively. FA treatment resulted in a reduction in the concentration of most free amino acids in lucerne silage, with the exception of glutamic acid and serine; the branched chain amino acids as well as glycine, tyrosine and proline were significantly (P<0·05) reduced after 3 d of ensilage. By contrast, FA treatment of maize did not significantly ( p <0·05) affect the NPN content nor was there a consistent pattern in the changes in FAA-N. Formic acid treatment of maize did produce a significant reduction ( P <0·05) in the concentration of most individual free amino acids at 90 d of ensilage. Overall, FA treatment had a more predictable effect on proteolysis and amino acid degradation in ensiled lucerne than in ensiled whole-plant maize.     

Fermentation and nutritive value of Zea mays and lucerne forage ensiled with added rolled barley

The effect of adding rolled barley on the ensiling characteristics and nutritive value of lucerne and corn ( Zea mays ) forage was studied using 19-1 plastic mini-silos. Other factors evaluated with lucerne were the effect of wilting and added lactic acid bacteria. Rolled barley was added at rates of 0, 50, 100 and 150gkg⁻¹ (wet weight) to lucerne or corn forage. Lucerne was ensiled at 170–330 g kg⁻¹ dry matter (DM) and lactic acid bacteria were applied at 10⁵g⁻¹ wet forage. Addition of rolled barley consistently improved the fermentation of lucerne by lowering the pH and decreasing the concentration of acetate and ammonia nitrogen. Wilting of lucerne had variable effects on fermentation in two experiments. The addition of lactic acid bacteria improved fermentation by decreasing the concentrations of acetate and ammonia nitrogen, improved the nutritive value by increasing the in vitro dry matter digestibility (IVDMD), and was associated with decreased amounts of lactic acid. Corn was ensiled at 250 g kg⁻¹ DM, and added rolled barley had little effect on the fermentation of corn silage. The digestibility of all silages determined in vitro was improved by the addition of rolled barley.     

Determination of microbial protein in perennial ryegrass silage

The microbial matter fraction was determined in perennial ryegrass silages of different dry-matter (DM) contents, ensiled with or without Lactobacillus plantarum . ¹⁵N-Leucine and the bacterial cell wall constituent diaminopimelic acid (DAPA) were used as markers for microbial-N. Perennial ryegrass crops with DM contents of 202, 280 or 366 g kg⁻¹ fresh weight were ensiled in laboratory-scale silos and stored for 3 to 4 months. At different times after ensiling, silages were analysed and microbial fractions were isolated. Microbial-N concentration determined with ¹⁵N-leucine reached a maximum during the first week of ensilage. It remained unchanged thereafter, except in silage with a DM content of 280 g kg⁻¹ in which it decreased ( P  < 0·01) by 32% during storage. After 3 to 4 months ensilage, microbial-N concentration varied from ≈0·3 to ≈1·7 g kg⁻¹ DM. A negative relationship was observed between microbial-N concentration and silage DM content. Inoculation resulted in an approximately twofold increase ( P  < 0·001) in microbial-N concentration. Microbial-N concentrations determined with DAPA were 1·14–2·07 times higher than those determined with ¹⁵N-leucine. However, 19–35% of the DAPA in silage occurred in a soluble form, indicating that this fraction of DAPA was not associated with intact bacteria.     

Silage and milk production: studies with diets containing white clover silage

In three separate feeding experiments using a total of twenty-six individually-housed Ayrshire cows, three wilted silages made from Blanca white clover were offered ad libitum with either different supplements or different proportions of grass silage. The clover silages contained 680 g white clover kg⁻¹ on a DM basis, and had a mean DM concentration of 263 g kg⁻¹ with 231 g CP kg⁻¹ DM and 91 g ammonia-N kg⁻¹ N. The pH values averaged 4·16 and the DOMD concentrations 611 g kg⁻¹. In experiment 1 the daily intake of clover silage given alone was 15.2 kg DM per cow, i.e. 30·1 g kg⁻¹ live weight, and decreased by 0·76 kg DM kg⁻¹ barley DM and by 0·66 kg DM kg⁻¹barley plus soybean meal DM when these feeds were offered as supplements. Milk yield and fat concentration were higher on the supplement treatments than on the clover silage-only treatment. In experiments 2 and 3 the intakes of silage and total DM increased as the weight of clover in the diet increased from 0 to 700 g kg⁻¹ with parallel increases in milk yield. The effects on milk composition were small and generally non-significant. Although white clover silages with excellent fermentations were made, it is concluded that the main role of white clover in a silage system will be in mixed swards with grass to reduce the input of fertilizer N and to increase the voluntary intake of silage.     

The effect of an acid salt-type additive on the fermentation of grass silages made in bunker silos on commercial dairy farms in Wales

The fermentation characteristics and chemical composition of 57 first-cut and 30 second-cut samples of grass silages, made in bunker silos on commercial dairy farms in Wales in 1990, and treated with a nominal 61 t⁻¹ of an acid salt-type additive at ensilage, is described. Typical chemical composition of grass cut for ensilage was 156 g kg⁻¹ dry matter (DM) and 28 g kg⁻¹ water soluble carbohydrate (WSC), with 181 g (kg DM) ⁻¹ crude protein (CP) and 232 g (kg DM) ⁻¹ modified acid detergent fibre (MADF). The effect of additive use was to produce silages with DM 230 g kg⁻¹ pH 3·93, ammonia N 70 g kg⁻¹ total N, with residual WSC 35 g (kg DM) ⁻¹, lactic acid 83 g (kg DM) ⁻¹, total acids 118 g (kg DM) ⁻¹ and butyric acid 0·7 g (kg DM) ⁻¹. No significant differences were found between first- and second-cut silages. Silage fermentation was restricted (i.e. lactic acid less than 60 g kg DM⁻¹) in only 20% of the samples.
It is suggested that on commercial farms the application rate achieved may be insufficient to produce a restricted fermentation.     

Effects of treating lucerne with an inoculum of lactic acid bacteria or formic acid upon chemical changes during fermentation, and upon the nutritive value of the silage for lambs

A second cut of lucerne was wilted to 500 g DM kg⁻¹ and either left untreated (control) or treated with formic acid (4.5 1 fresh forage t⁻¹) or with a commercial inoculum of lactic acid bacteria (10⁵ colony forming units (cfu) g forage⁻¹). The forages were ensiled in 2-t capacity silos for 8 months, and later fed to six lambs (mean initial weight 27.7 ±1.60 kg) in a 3x3 duplicated Latin square with 27-d periods. Portions of the untreated and additive-treated forages were also ensiled in laboratory silos at 25 ° C for intervals up to 42 d. Results from the laboratory silos showed that the major increase in ammonia-N in silage occurred between 40 h and 7 d of fermentation; during this period, both formic acid and the inoculant produced a smaller increase in ammonia-N, than did the control. The pH of inoculated silage declined from 5.74 to 4.57 in 7 d, but it took 14 d for the pH of the control silage to fall below 5.0. Formic acid treatment immediately reduced the silage pH from 5.74 to 5.10 ( P < 0·01); the pH then remained unchanged until 21 d, after which it decreased slightly. When compared with control, lambs fed formic acid-treated silage consumed more ( P < 0·05) digestible organic matter; the response was associated with a trend towards decreased concentration of ammonia in plasma. Inoculation of lucerne silage did not ( P < 0·05) affect voluntary intake but increased ( P <0.05) apparent digestibility of fibre and tended to increase N retention.     

A comparison of unwilted and wilted grass silages offered to beef cattle without and with monensin sodium

A randomized block experiment was conducted to compare unwilted and wilted grass silages and the effects of the feed additive monensin sodium on the silage intake and performance of finishing beef cattle. Two regrowths from a predominantly perennial ryegrass (cv. S24) sward were ensiled either without wilting or after field wilting for 3 d (dry matter (DM) concentrations 161 and 266 g kg⁻¹ respectively). Both silages were treated with formic acid (2·6 and 30 litre t⁻¹ respectively) and were well preserved. The silages were offered ad libitum to forty-eight Charolais-cross cattle (thirty-two steers and sixteen heifers, mean initial live weight 351 kg) for 145 d. All animals received 2·2 kg concentrates per head daily and half of those on each silage treatment received in addition 200 mg monensin sodium per head daily. Silage DM intake was 5.04, 504. 5·48, 5·63 ± 0.134 kg d^−l; fasted liveweight gain was 0·69, 0·77. 0·64 and 0·73 ± 0.033 kg d^−l and carcass gain was 0·47, 0·50, 0·40 and 0·45 ± 0·020 kg d⁻¹ for the unwilted silage without and with monensin and the wilted silage without and with monensin respectively. It is concluded that wilting grass of low DM concentration for 3 d prior to ensiling reduced the performance of finishing beef cattle below that obtained from well-preserved unwilted silage in spite of a higher DM intake being achieved with the wilted silage. The inclusion of monensin sodium in a silage-based diet increased performance without significantly affecting feed intake.     

Effect of herbage water-soluble carbohydrate content and weather conditions at ensilage on the fermentation of grass silages made on commercial farms

A total of 1009 samples of silage made in bunker silos on commercial farms between 1972 and 1978 was analysed to investigate the effect of herbage water-soluble carbohydrate content (WSC) and weather conditions at ensilage on fermentation as measured by ammonia-N concentration and pH of first-cut grass silages.
Silage dry matter (DM) content had the major effect on fermentation. Factors influencing silage DM were rainfall and hours of sunshine during silage making, and DM content of the grass cut. WSC content of herbage ensiled also had a significant effect on subsequent fermentation. The major influences on herbage WSC were hours of sunshine and rainfall during the growing season.
The effect of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of silage DM.
The minimum DM necessary to produce well-fermented silage without additive was approximately 260 g kg⁻¹. Use of formic acid significantly reduced this requirement to 240 g kg⁻¹ and to 252 g kg⁻¹ for sulphuric add + formalin. The results indicate that the minimum herbage WSC necessary to prevent a clostridial fermentation developing in silage with a DM content of 230 g kg⁻¹ is approximately 37 g kg⁻¹ without additive and 30 g kg⁻¹ with formic acid.
It is concluded that on commercial farms, weather conditions i.e. amount of rainfall and sunshine prior to and at ensilage, have a greater effect on subsequent silage fermentation than additive use.     

The effect of dry matter content on the preservation of big hale grass silages made during the autumn on commercial farms in South Wales 1983-87

A total of eighty-nine big-bale (BB) silages made on commercial farms in South Wales during the autumn in 1983-87 were analysed to discern the effect of dry matter (DM) content on fermentation. Silages were made predominantly in late September, during fine weather from permanent pasture or perennial ryegrass pastures, which had not been grazed for 9 weeks. On average 7·8 ha of pasture were cut; most farmers attempted to pre-wilt grass for an average of 36 h prior to ensilage in individual plastic bags. Silage making was usually completed within 4 d of starting to cut. Silage ammonia-N content was inversely correlated with DM content. The results indicated that a DM content of about 280 g kg⁻¹ was needed to produce well-preserved silage with an ammonia-N content of 100 g (kg total N)⁻¹. Silages were, therefore, generally poorly preserved because the DM increase from wilting was only 2·3 g kg⁻¹ h⁻¹.     

Effect of stage of maturity of whole-crop barley on intake and liveweight gain by dairy steers differing in initial live weight

The effect of maturity at harvest of whole-crop barley for ensiling on intake and liveweight gain of dairy steers differing in initial live weight (LW) was evaluated in an experiment over two years. Light (104–120 kg) and heavy (402–419 kg) dairy steers were fed diets containing predominantly whole-crop barley silage harvested at the milk stage [dry matter (DM) content of 284 g kg⁻¹ and neutral-detergent fibre (NDF) concentration of 526 g kg⁻¹ DM] or the dough stage of maturity (DM content of 328 g kg⁻¹ and NDF concentration of 445 g kg⁻¹ DM) and supplemented with up to 1 kg of concentrate. Dry matter intake (g kg⁻¹ LW) was higher for whole-crop barley harvested at the dough stage than at the milk stage of maturity and the difference was greater in heavy than in light steers ( P <  0·001). Liveweight gain was higher and feed conversion ratio was lower for dough-stage compared with milk-stage silage ( P  <   0·05) but there was no interaction with size of steer. Whole-crop barley harvested at the dough stage of maturity promoted higher liveweight gains in dairy steers compared with whole-crop barley harvested at the milk stage due to a higher DM intake.     

Ensiling of manured crops—effects on fermentation

The quality of silage from crops fertilized with cattle manure and an inorganic fertilizer was compared in experiments from 1985 to 1989. Manure was spread either as farmyard manure (FYM, 25t ha⁻¹) or as slurry (20-50t ha⁻¹). Crops were direct cut (approximately 200 g DM kg⁻¹) or wilted (approximately 300 g DM kg⁻¹), precision chopped and ensiled in experimental silos. Silage was treated with 4 kg 85% fonnic acid t⁻¹ fresh matter (FM), an inoculant or no additives. The use of manure, particularly FYM, resulted in more Bacillus spores on crops at harvest compared with fertilized crops. Clostridium spores increased as a result of manuring in 1989 only on FYM-treated crops. Differences in the chemical composition of crops were usually small between fertilizer treatments. The quality of silage from slurry-dressed crops, compared with that of silage from fertilized crops, varied between years. The FYM resulted in reduced silage quality, i.e. high pH values (> 4·5), high ammonia N (> 150 g kg⁻¹ total N) and butyric acid (> 6·3 g kg⁻¹ water) concentrations, and high numbers of Bacillus (10⁵ g⁻¹ FM) and Clostridium spores (10⁵ g⁻¹ FM). The concentration of lactic acid was low (≤ 12 g kg⁻¹ water). Wilting and additives generally improved silage quality and reduced the differences between treatments. However, the efficiency of the inoculant on farmyard manured crops was limited.     

Silage and milk production: studies with molasses and formic acid as additives for grass silage

In two separate feeding experiments using a total of twenty-four individually housed Ayrshire cows six silages made from perennial ryegrass were offered ad libitum with supplements of concentrates. In Experiment I herbage with a dry matter (DM) concentration of 225 g kg⁻¹ received either formic acid ('Add-F') at the rate of 2·0 litres t⁻¹ or undiluted cane molasses at rates of 10, 20 and 30 litres t^−l; the mean daily silage intakes were 9·54, 908, 9·27 and 9·49 kg DM per cow and the daily milk yields, corrected to 40 g fat kg⁻¹, were 23·2, 22·3, 22·8 and 22·9 kg per cow respectively but none of the differences between the four treatments was significant. In Experiment 2 herbage with a DM concentration of 269 g kg⁻¹ received formic acid at a uniform rate of 2·6 litres t⁻¹ either with or without an additional application of molasses at 20 litres t⁻¹; the mean daily silage DM intakes were 8·70 and 9·28 kg per cow and the daily fat-corrected milk yields were 22·2 and 21·9 kg per cow respectively and were not significantly different. In both experiments the effects of the treatments on milk composition were small and not significant. It is concluded that there were no advantages in applying molasses to herbage treated with formic acid, and that the rate of application of molasses to untreated herbage which equated with the formic acid application was 20·30 litres t^−l when assessed on the basis of silage composition, intake and milk production.     

A study of the efficacy of a bacterial inoculant and formic acid as additives for grass silage in terms of milk production

In a two-year experiment, three silages were prepared from herbage treated either with an inoculant at 1·25 × 10⁵ organisms (g fresh material (FM))⁻¹. formic acid (850 g kg⁻¹) at 4 1 (t FM)⁻¹, or no additive (untreated). In Experiment 1, unwilted and in Experiment 2, wilted silages were investigated and had mean dry matter (DM) and water soluble carbohydrate (WSC) concentrations at ensiling of 171 g kg⁻¹ and 17·6 g (kg FM)⁻¹ and 263 g kg⁻¹ and 25·1 g (kg FM)⁻¹, respectively. In Experiment 1, 45 and in Experiment 2, 54 individually fed cows were used to evaluate the silages in three-treatment, randomized-block design experiments. During weeks 4-12 of lactation the cows were offered silages ad libitum and during weeks 15-26 a constant amount of silage was fed. There were few major differences in chemical composition of the resulting silages. Formic acid had no effect on silage digestibility. Inoculant treatment increased digestibility when the grass had been wilted. The use of formic acid resulted in increased silage DM intake of 9% during weeks 4-12 of lactation in Experiment 1 but not in Experiment 2. The inoculant gave no increase in silage DM intake over the control in Experiment 1 but increased silage DM intake by 7% in Experiment 2. There was no significant response in milk yield to formic acid. In Experiment 2 the response in milk yield to inoculant treatment was significant both in weeks 4-12 of lactation (4%) and in weeks 15-26 of lactation (5%). It is concluded that the response in milk yield to the use of a specific inoculant appears to be mediated through increased intake of metabolizable energy (ME).     

Influence of bacterial inoculant and substrate addition to lucerne ensiled at different dry matter contents

Field-wilted lucerne was chopped with a forage harvester at 33 ± 1·5, 43 ± 2·0 and 54 ± 1·8% dry matter, treated and ensiled in laboratory silos during four cuttings in each of two years. Treatments were control (C), sugar addition at 2% of fresh weight (S), inoculum applied at 3 × 10⁵ bacteria g⁻¹ herbage (I), and sugar and inoculum combined (IS). Duplicate silos were opened and analysed after 1, 2, 3, (4 or 5), 7, 14 and 60 d of fermentation. The initial rate of proteolysis of lucerne protein decreased with increasing dry matter (DM) content of the lucerne, and was not influenced by the year, cutting or silage treatment. Inoculation increased ( P <0·05) the rate of pH decline for all silage dry matters, and shortened the lag time prior to pH decline with 33 and 43% dry matter silages. Sugar addition had no effect on rate of pH decline or lag time. Inoculation and sugar addition both lowered final pH, acetic acid, ammonia (NH₃), free amino acids (FAA) and soluble non-protein N (NPN) in silages ( P <0·01) and increased lactic acid content with 33 and 43% dry matter silages. Only inoculation was beneficial at 54% DM with no difference between I and IS. The influence of forage characteristics (epiphytic lactic acid bacteria, buffer capacity and sugar:buffer capacity ratio) on treatment effectiveness varied with dry matter content.     

A study of the nutritive value of white clover (Trifolium repens L.) in relation to different stages of phenological maturity in the primary growth phase in spring

This paper reports the effects of onset of phenological maturity on the nutritive value of white clover ( Trifolium repens L.). The study comprised (i) examination of an extensive data set on nutritive value and (ii) investigation of the constituents of nutritive value, in vivo feeding value, protein degradability and metabolizable protein content of white clover harvested at three stages of maturity (early-flowering, full-flowering, ripe seed stages) during the primary growth phase in spring in Australia. The data set on nutritive value showed a consistent pattern of high nutritive value during cool season months, progressive decline through spring and uniformly lower nutritive value over summer. Results from laboratory determinations, in sacco degradability studies and a digestibility trial on white clover harvested at early-flowering, full-flowering and ripe seed stages were consistent with results from the data set on nutritive value. Onset of maturity during the primary growth phase in spring was accompanied by large changes in nutritive value: neutral-detergent fibre (NDF) increased from 184 to 301 g kg⁻¹ dry matter (DM), nitrogen (N) declined from 36 to 20 g kg⁻¹ DM, in vitro digestibility declined from 0·74 to 0·65 and metabolizable protein content declined from 144 to 67 g kg⁻¹ DM from early-flowering to ripe seed stage. These nutritive value changes were accompanied by a decline of in vivo digestibility at the rate of 0·0032 d⁻¹ and an 0·2 reduction in voluntary intake.     

Comparison of a straw/concentrate mixture with silage as a buffer feed for grazing dairy cows

Over a 24-week period during the 1986 summer, three groups of January- to March-calving dairy cows were either grazed conventionally (G) or grazed between morning and afternoon milkings and housed overnight and offered grass silage (Si) or a straw/concentrate mixture (St) ad libitum. The straw/concentrate mixture contained proportionately, 0-33 long barley straw, 0·28 ground barley, 0·12 soya bean meal, 0·25 molaferm and 0·22 minerals. The metabolizable energy (ME) and crude protein (CP) contents of the silage fed in weeks 1-8 and weeks 9-24 were 9-5 and 10⁻⁶ MJ kg MD⁻¹ and 160 and 191 g kg DM⁻¹ respectively. The straw mix had an ME content of 10⁻¹ MJ kg DM⁻¹ and CP content of 134 g kg DM⁻¹. Partial storage feeding with silage or a straw/concentrate mixture led to a decrease in estimated herbage DM intake. The feeding of the straw/concentrate mixture increased total DM intake, but the estimated total ME intake was similar for treatments G and St. The intakes (kg DM d⁻¹) for treatments G, Si and St were respectively, herbage 11·7,6·8,4·1; total l3·5,13·6,15·0; total ME intake (MJd⁻¹) 163, 155, 163.
Animal performance was, for treatments G, Si and St respectively: milk yield (kg d⁻¹) 19·2, 17·5, 19·1 (s.e.d. 0-87); milk fat content (g kg⁻¹) 36·9, 37·6, 37.1 (s.e.d. 1.22); milk protein content (g kg⁻¹) 35·3, 32·9, 33·4 (s.e.d. 0·76).     

The chemical composition, digestibility and energy value of fermented and urea-treated whole crop wheat harvested at three stages of maturity

The metabolizable energy (ME) content, digestibility in vivo and chemical composition of fermented and urea-treated whole crop wheat (WCW) forage harvested were measured at different maturities. Over 2 years, twenty-four wheat forages (cv. Slepjner, Hussar and Cadenza) were harvested at 376, 516 and 632 g dry matter (DM) kg⁻¹ in Year 1 and 341, 467 and 544 g DM kg⁻¹ in Year 2 (Cuts 1, 2 and 3 respectively). Forages were conserved in 200−-ι barrels with or without a formic acid-based additive and with urea in Cuts 2 and 3 (20 or 40 g kg⁻¹ DM). Chemical compositions, digestibility in vivo and energy losses in faeces, urine and methane were measured in wethers fed 12 g DM kg⁻¹ live weight. Respective ranges in pH, crude protein, water-soluble carbohydrates, starch and neutral detergent fibre plus amylase (NDFA) contents were 3·8–8·5, 89–394, 2–43, 23–424 and 306–655 g kg⁻¹ DM. ME content, digestible organic matter content (DOMD) and digestibilities of starch and NDFA ranged between 8·4 and 15·7 MJ kg⁻¹ DM, 558 and 708 g kg⁻¹ DM, 0·901 and 0·999 and 0·362 and 0·693 respectively. Predicted methane energy losses were poorly correlated with measured values ( r ² < 0·45) in both years, but this accounted for less than 0.1 MJ ME kg⁻¹ DM. Significant ( P < 0.05) effects of maturity on the ME/DOMD ratio were observed and ascribed to differences in the extent of fermentation. Therefore, treatment application and maturity exert considerable influence on the ME content of WCW.     

Agronomic performance and nutritive value of common and alternative grass and legume species in the Peruvian highlands

The agronomic performance and nutritive value of twelve annual and perennial grasses and legumes were analysed in order to define alternatives to local forages for dry-season feeding of ruminants in the Peruvian Andes. There were twelve species and two fertilizer treatments (no fertilizer and a N;P;K fertilizer mainly applied at sowing) in an experiment with a randomized complete block design with three replicates at each of two sites. Plant height, soil cover by forage and weed species, frost damage, dry matter (DM) yield and nutritive value of herbage were evaluated in 2005 and 2006. Among the annual species, Hordeum vulgare L. cv. UNA 80 and × Triticosecale Wittm. had the highest DM yields when fertilized (8226 and 6934 kg ha⁻¹ respectively). Without fertilizer the alternative cultivars had similar DM yields to that of the local forages. Cultivars of Avena sativa L. had lower concentrations of neutral-detergent fibre (NDF) (557 g kg⁻¹ DM) and higher concentrations of predicted net energy for lactation (5·86 MJ kg⁻¹ DM) than the other annual grass species (625 g kg⁻¹ DM and 5·01 MJ kg⁻¹ DM respectively), while the legumes were superior in concentrations of crude protein (277 g kg⁻¹ DM) and NDF (362 g kg⁻¹ DM). Considering the low agronomic performance of the perennial forages, a mixture of fertilized annual grasses and legumes appears the most appropriate approach to meeting the demand for forage of high nutritive value in the Peruvian highlands.     

A note on ensiling safflower forage

Abstract The ensiling characteristics of safflower ( Carthamus tinctorius ) wilted to 290 and 411 g dry matter (DM) kg⁻¹ fresh material were studied in 1·5 l glass jars. The experiment included a control and the application of Lactobacillus plantarum at 3·3 × 10⁵ colony-forming units (cfu) per g of crop. After 60 days of ensiling, the pH of safflower silages was 4·6 and 4·0 in the control and inoculated silages respectively, with corresponding values for lactic acid, the major fermentation product, of 20 and 45 g kg⁻¹ DM. The silages from the anaerobic jars were stable upon aerobic exposure. It is concluded the safflower silage has potential as an alternative fodder in semiarid regions.     

Seasonal changes in the crude protein concentration of mixed swards of white clover/perennial ryegrass grown without fertilizer N in an organic farming system in the United Kingdom

Changes in the crude protein (CP) concentration of white clover and perennial ryegrass herbage from a mixed sward were determined on six sampling dates from May to October in each of 2 years. The swards were grown without fertilizer N in an organic farming system and continuously grazed by dairy cows during the grazing season. The annual mean contents of white clover in the dry matter (DM) of the sward were 272·3 and 307·0 g kg⁻¹ in Years 1 and 2. The mean CP concentrations of the white clover and perennial ryegrass herbage were 251·6 and 151·9 g kg⁻¹ DM in Year 1 and 271·9 and 174·0 g kg⁻¹ DM in Year 2 respectively. The CP concentration of the white clover increased significantly during the grazing season from 220·0 to 284·1 g kg⁻¹ DM in Year 1 and from 269·0 to 315·5 g kg⁻¹ DM in Year 2. In the perennial ryegrass herbage the CP concentration increased from 112·2 to 172·6 g kg⁻¹ DM in Year 1 and from 142·7 to 239·5 g kg⁻¹ DM in Year 2. The rate of increase during the season in the CP concentration of the perennial ryegrass herbage was similar to the rate of increase recorded in the white clover herbage.