Spring Frost Damage on Young Picea sitchensis 1. Occurrence of Damaging Frosts in Scotland Compared with Western North America

Air frosts (in Stevenson screens) of –2.5°C or belowin Scotland were judged to be potentially damaging to the newlyemerging shoots on young trees of Picea sitchensis at the timeof budburst. This was based on a knowledge of the killing tissuetemperature and the relationship between air and grass minimain May. Dates of last spring air frosts of –2.5°C and –4.5°Cwere obtained for 42 meteorological stations in northern Britainwith runs of 19 to 116 years. Frequency distributions of lastfrost dates were approximately normal. About 80% of the variationbetween stations could be attribuoted to altitude, distancefrom the sea and latitude. The variance in last dates of –2.5°Cfrosts decreased from mild to cold sites. Multiple regressionswere used to produce maps of last frost dates in 20x20 km gridsquares in Scotland. Dates of last 28° F (–2.2°C) and 24°F (–4.4°C)frosts were obtained for 20 sites in western North America,spanning the natural range of P. sitchensis. Mean dates increased,and variances decreased, from south to north. Most Scottish upland plantation sites (e.g. Glentress and Eskdalemuir)experience –2.5°C air frosts until later in the yearthan all coastal stations in western North America south ofCordova, Alaska. On average, Eskdalemuir (242 m altitude) experiences–2.5°C air frosts about 4 weeks later in the springthan Masset (3 m) on the coast of the Queen Charlotte Islands,which has been the source of much of the P. sitchensis plantedin Scotland. Masset has a spring frost climate somewhat milderthan Durham.     

Autumn Frost Damage on Young Picea sitchensis 2. Shoot Frost Hardening, and the Probability of Frost Damage in Scotland

The natural increase in frost hardiness of detached shoots ofPicea sltchensis during August to November was measured usinga programmable freezing chamber. Oregon, Queen Charlotte Islandsand Alaskan provenances were compared, and the effects on hardeningof long days, warm temperatures and frosts were determined.A computer model was constructed to mimic the observed patternsof autumn frost hardening, as functions of air minimum temperatures,daylengths and the occurrence of frosts. The model was used(a) to describe the pattern of autumn frost hardening at differentsites in northern Britain, using past meteorological records,and hence (b) to determine when frosts occurred that might havedamaged young trees. The model accurately predicted known instancesof autumn frost damage at Kirroughtree and Carnwath. The predicted probability of autumn frost damage on young treesof P. sitchensis in upland areas of Scotland was much lowerthan that previously predicted for spring frost damage. Theestimated return time for autumn frost damage to an Oregon provenanceat Eskdalemuir was 8.3 years, and the return time for a Q.C.I.provenance was longer than 10 years. Most damaging frosts occurredin October, but frosts like those on 13–15 October 1971,which followed warm weather and caused wide spread damage inScotland, have been quite rare. Alaskan provenances would rarelybe damaged by autumn frosts, nor would trees of Q.C.I. provenancegrowing in lowland areas of Scotland, or at Masset on the QueenCharlotte Islands.     

Spring Frost Damage on Young Picea sitchensis 2. Predicted Dates of Budburst and Probability of Frost Damage

The probability of frost damage at the time of budburst on thelateral shoots of young trees of Picea sitchensis in Scotland,and at Masset on the Queen Charlotte Islands, was determinedfrom (a) the dates of budburst, predicted from past temperaturerecosds using a thermal time / chilling model, and (b) the datesof last air frosts of –2.5°C. The mean date of budburst occurred between 6 and 13 May at arange of Scottish sites up to about 250 m altitude, but it occurredabout 6 days later for each further 100 m increase in altitudeup to 550 m. This resulted from an interplay between chillingand thermal time requirements for budburst. In different years,budburst dates ranged from mid-April to early June. From 1920to 1960 there was a trend towards warmer springs and earlierdates of budburst, which has been reversed since 1960. Potentially damaging frosts have occurred near the date of budburstonce every 3 years (on average) at Eskdalemuir and Braemar duringthe last 66 to 107 years, and once every 3 to 5 years in manyupland plantation regions of Scotland. The probability of damagingfrosts was greatest in the period 1930 to 1960 when budburstoccurred relatively early, and has been relatively small since1960, because budburst has occurred relatively late. Renewedwarming of the climate in future may increase the probabilityof damaging frosts coinciding with the time of budburst. Masset, on the Queen Charlotte Islands, from where Britain hasimported most P. sitchensis seed, has relatively cool springsso that budburst occurs relatively late (averaging 18 May).Also there are few damaging frosts at Masset after late April.Consequently, potentially damaging spring frosts have occurredthere only once in 65 years.     

Seasonal Changes in the Frost Hardiness of Provenances of Picea sitchensis in Scotland

Changes in the natural level of frost hardiness of shoots offour provenances of Picea sitchensis were monitored over twogrowing seasons by detaching shoots from 7 to 10-year-old treesgrowing in a nursery in Scotland, and subjecting them to freezingtemperatures under conditions which simulated night frosts. Six seasonal phases of frost hardiness were identified (Fig.3).

1. During each autumn, killing temperatures (the level of hardiness)decreased from –5°C to below –20°C, beginningseveral weeks after shoot elongation ceased. Alaskan provenanceshardened in September, apparently in response to shorteningday lengths alone, whereas an Oregon provenance did not hardenuntil November, after repeated frosts. Queen Charlotte Islandsprovenances were intermediate.
2. From November to March allprovenances were hardy to below –20°C,which is adequateto prevent direct freezing injury at mostplantation sites.
3. In March-April, several weeks before bud-burst, old shootsdehardenedto killing temperatures of about –10°Cin responseto warm temperatures, and southerly provenancesdid so beforenortherly ones.
4. During bud-burst the newly-emergingshoots were hardy to only–3°C to –5°C untilthey were about 3.5 cmlong. All provenances burst bud at thesame time and were equallyfrost susceptible at this time.
5. DuringMay-July the elongating shoots fluctuated in hardinessbetween–5°C and –10°C apparently in responsetofluctuating ambient temperatures.
6. In August 1980 there wasa period of late summer dehardeningto killing temperaturesof about –3°C.

Seasonal changes in hardiness are discussed in relation to changesin shoot growth and environmental factors. The main opportunitiesfor selecting frost hardy genotypes seem to be in the rate ofautumn hardening, the time of pre-bud burst dehardening, andthe time of bud-burst.     

Frost Hardiness of Nothofagus procera and Nothofagus obliqua in Britain

The frost hardiness of four seedlots of Nothofagus proccra andsix seedlots of Nothofagus obliqua was measured experimentallyduring three winters. Shoots were taken from saplings growingat the Bush Estate in Scotland. All seedlots set buds in lateSeptember, hardened very slowly in the autumn, were damagedto some extent by temperatures below –14°C in mid-winter,and dehardened during frosty weather in February/March priorto budburst in mid to late April. The range of mid-winter temperaturesgiving 0%, 50% and 100% kill (LT₀, LT₅₀ and LT₁₀₀) were 8–14°C,13%20°C and 14–22°C, respectively. By contrast,British Fagus sylvatica hardened off rapidly in September, wasundamaged by frosts well below –20°C in mid-winter,and did not deharden until late April, prior to budburst atthe end of May. Nothofagus seedlots from Nuble in Chile (the most Equatorialsource) were the most frost susceptible: unfortunately, seedof this origin was supplied to many British nurseries between1976 and 1W9. Seedlots from Neuquen in Argentina, and from maturetrees of Malleco (Chile) origin growing in Britain, were themost host hardy. N. procera tended to be hardier than N. obliqua,and the trees became hardier with age. Past temperature records for Britain suggested that all theNothofagus seedlots had a high risk of suffering severe frostdamage at least once during a timkr rotation in all but mildcoastal regions. Spring and autumn frosts may be more damagingthan winter frosts. However, if it were possible to select individualswithin populations that were 3–6°C more frost hardythan the population means, such trees would be sufficientlyhardy to avoid frost damage in most lowland regions.     

Frost Hardiness of Red Alder (Alnus rubra) Provenances in Britain

The phenology and frost hardiness of shoots of 15 provenancesof Alnus rubra growing in Scotland were measured over one autumn,winter and spring. Dates of budset (in September) and the onsetof rapid frost hardening (in October-November) occurred about2 days earlier for each degree latitude of origin northwards,except for an Idaho provenance. However, all provenances dehardenedat about the same time in March and burst their buds between8 and 14 April. Assuming that rapid frost hardening in the autumnwas triggered primarily by shortening daylengths, Alaskan provenancesof A. rubra seemed better adapted to British conditions thansouthern British Columbian provenances, which have been mostcommonly planted. However, even Alaskan provenances are proneto spring frost damage. Scottish A. glutinosa and Alaskan A.sinuata set buds and frost hardened 1–2 weeks before eventhe Alaskan A. rubra, and burst their buds 2–3 weeks laterin April-May. All three species were hardy to below –30°Cfrom December to mid-March.     

Performance and Frost Hardiness of Picea sitchensis x Picea glauca Hybrids in Scotland

The growth of seven Picea sitchensis x Picea glauca hybridswas compared with the growth of two P. sitchensis provenancesand Picea glauca var.albertiana at two sites in northern Scotland.The sites were at Aultmore (an exposed, dry site with a mineralsoil) and Shin (a frosty, wet site with deep peat). They wereof the type considered more suited to Pinus contorta than P.sitchensis. At age 10, in 1984, most of the hybrids, at both sites, wereabout 10 and 20 per cent taller than P. sitchensis of Masset(Q.C.I.) and Ketchikan (Alaska) provenance, respectively. P.glauca var. albertiana grew very poorly, especially at Aultmore. At Aultmore, the frost hardiness of three of the tallest hybrids,the two P. sitchensis provenances, and P. glauca var.albertiana,was tested at about 3-weekly intervals throughout 1982 and 1983.Detached shoots were subjected to artificial frosts in a programmablechamber. P. glauca var. albertiana was frost susceptible atbudburst, but at all other times it was relatively very frosthardy (eg. to^–10°C in mid-August). Also, the hybridswere consistently more hardy than P. sitchensis of even Ketchikan(Alaska) provenance from July onwards. However, the hybridswere less frost hardy than P. sitchensis of Masset (Q.C.I.)provenance in early spring (they dehardened a week earlier inMarch-April) and their buds were equally as frost susceptibleat the time of budburst. In 1983, trees of P. glauca var. albertianaburst their buds about a week sooner than P. sitchensis. It was concluded that P. sitchensis x P. glauca hybrids canperform better than P. sitchensis at sites considered ‘marginal’for P. sitchensis, and that their good performance may be partlyattributed to, or associated with, their greater summer andautumn frost hardiness. A programme of inter-specific hybridizationis being pursued.     

Frost Hardiness of Subalpine Eucalypts in Britain

Laboratory freezing tests were used to determine seasonal changesin the frost hardiness of detached shoots of young trees ofEucalyptus gunnii (from central Tasmania), and E. niphophilaand E. debeuzevillei (‘snow gums’ from the SnowyMountains, Australian Capital Territory). The trees were growingat the Bush Estate. No difference was found between the speciesor between seedlots, all of which were from high altitudes nearthe tree line. In midwinter (February) the shoots tolerated –16°Cwithout suffering damage, and many shoots survived temperaturesas low as –18°C to –22°C. This result agreedwith Evans' (1986) observation that some trees within theseseedlots survived temperatures in the range –19°Cto –23°C in field planting during the winter 1981/82.In their native habitats the trees rarely experience temperaturesbelow –20°C. The shoots did not harden appreciably before they experiencedfrosts (in late October/early November) and so may be proneto autumn frost damage. By contrast, they were slow to dehardenin late winter and spring and did not seem prone to spring frostdamage. There were no killing air frosts during the period of this study(winter 1985/6), but many trees died, possibly as a result ofground freezing, producing root injury and/or shoot desiccation.In subalpine regions of Tasmania and the Snowy Mountains theground is covered by snow throughout the winter. Variation in frost hardiness within these hardy seedlots couldbe exploited.     

Winter Frost Hardiness of two Chilean Provenances of Nothofagus procera in Scotland

The frost hardiness of the shoots of individual trees withintwo Chilean provenances of Nothofagus procera (Poepp & Endl.)Oerst. was measured once in each of the months January, February,November and December 1989 and January and February 1990. Therewere significant (P<0.05) differences of frost hardinessbetween provenances but only one tree could be shown to be significantlymore frost hardy than the others within the same provenance.During the winter of 1989/90 both provenances were hardy toabout –14°C (temperature killing 50 per cent of shoots)in December, but the shoots dehardened to about –9°Cin January before hardening again in February. This patternof alternate hardening and dehardening seemed to mirror changesin air temperature and could render N. procera liable to frostdamage where (as happened in 1988/9 in the UK) mild spells occurin winter followed by severe frosts.     

Sitka Spruce and Douglas fir Seedlings in the Nursery and in Cold Storage: Root Growth Potential, Carbohydrate Content, Dormancy, Frost Hardiness and Mitotic Index

Seedlings (transplants) of 2+1 Sitka spruce (Picea sitchensis(Bong.) Carr.) and 1 + 1 Douglas fir (Pseudotsuga menziesii(Mirb.) Franco) were grown in a nursery at the Bush Estate,Scotland. Batches were lifted and cold stored at 0.5°C inNovember, December and January. Changes in growth, shoot apicalmitotic index, root growth potential (RGP), carbohydrate content,bud dormancy and shoot frost hardiness were monitored throughoutthe winter by taking samples at intervals from the nursery andfrom cold storage. Frost hardening occurred during the later stages of bud development(as mitotic indices decreased); autumn hardening was arrestedwhen seedlings were put in cold store, and some dehardeningoccurred in cold storage, especially in spring. Bud dormancystarted, and was greatest, just after bud growth (mitotic activity)virtually ceased; chilling in cold store was almost as effectivein releasing dormancy as natural chilling. The concentrationof total nonstructural carbohydrates stayed more or less constantat 100–150mg g^–1 from September to April in thenursery; in cold storage carbohydrates were depleted at 0.4–0.6mgg^–1 d^–1 (corresponding to respiration at 0.03–0.05mgCO₂ g^–1 h^–1) until there was only 40–50mgg^–1. Root growth potentials in the nursery increased in December,once the buds ceased growth, became dormant and had receivedsome chilling. Sitka spruce was ‘storable’ in November,before RGPs increased, but they then failed to achieve maximalfrost hardiness or ROP. Winter RGPs were high in Sitka spruceand were increased or maintained in cold storage, whereas RGPswere low in Douglas fir and decreased immediately after storage(except when stored in January). By the end of April, the RGPof cold stored Sitka spruce was much higher than that of directlifted plants. ROP changes in the nursery and in cold storagewere not consistently related to changes in seedling carbohydratecontents, shoot frost hardiness or bud dormancy. In practical terms, it was concluded that (1) the optimum dateto start lifting bare- rooted conifer transplants in the autumnis when their shoot apical mitotic indices have decreased tonear zero, and their RGPs have risen sharply; (2) high RGPsmay depend as much on the morphology of the roots (e.g. numberof undamaged root apices) as on the physiology of the shoots(e.g. carbohydrate status, dormancy and frost hardiness); and(3) in spring, transplants kept in cold storage since November,December or January are more frost hardy, slightly more dormant,and (in May) have higher RGPs than transplants lifted from thenursery.     

Spring Frosts Affecting the Establishment of Second Rotation Crops in Thetford Chase

Spring frosts in Thetford Chase cause a major reduction in thegrowth of young Corsican pine and very severe frosts or repeatedfrosting may kill the trees. Records of temperatures taken ona range of sites and in different conditions have shown that(a) spring frosts occur every year at Thetford, but the severefrosts which kill trees outright may only occur once every fewyears, (b) frost damage to plants mainly occurs when the treesare less than 18 in (45 cm) tall, (c) forest clearings largerthan 5 acres (2 ha) do not act as artificial frost hollows,(d) cold air flows down slopes of more than 1° and accumulatesat the bottom, forming a frosty zone. Studies showed that frost damage can be minimized by (a) completecultivation, (b) deep ploughing, (c) underplanting, and (d)strip felling. The benefits of these measures are demonstratedby temperature records and by measurements of tree growth. Methods of reducing frost damage are necessary in areas proneto spring frosts if crops of Corsican pine are to be successfullyestablished at low cost.     

Phenologies of sixteen European provenances of sessile oak growing in Scotland

Budburst, budset, leaf yellowing, growth, and frost damage wereassessed on 2- to 4-year-old transplants of 16 provenances ofsessile oak (Quercus petraea) collected in six European countriesand grown in Scotland. There were significant differences indates of budburst between provenances. Provenances from Franceand Austria burst bud earlier than the others (P > 0.05). Budburst date was negatively correlated with altitude of provenanceorigin but positively correlated with latitude, indicating thatsouthern provenances burst bud earlier than northern provenancesand are therefore more prone to spring frost damage. Although there were no significant differences of growth betweenprovenances, French provenances tended to grow later in autumnthan provenances originating in other countries. Consequentlyplants from these French provenances were more badly damagedby autumnal frosts (P > 0.05) than plants from other provenances.Budset and leaf yeljowing in late September were positivelycorrelated.     

Effects of nitrogen fertilization and temperature on frost hardiness of Aleppo pine (Pinus halepensis Mill.) seedlings assessed by chlorophyll fluorescence

In a 14-week study, 1-year-old Aleppo pine seedlings were grownin two growth chambers. Seedlings were artificially hardenedby decreasing photoperiod and temperature. In each chamber halfof the seedlings were fertilized with nitrogen (8.4 mg seedling^–1).In order to determine the relative importance of the hardeningenvironment versus fertilization, each chamber was programmedto decrease night temperatures down to a low of 8 or 4°C.Chlorophyll fluorescence and frost hardiness was measured fivetimes during the experiment. A sample of seedlings from eachtreatment was exposed to an artificial frost at –5°Cand the freezing effects were assessed by measurements of chlorophyllfluorescence and visual evaluation of needle damage. Seedlingsincreased their frost hardiness during the experiment in allthe treatments but the ratio of variable to maximal chlorophyllfluorescence (F_v/F_m) measured before freezing did not vary duringthe experiment. This indicates that Aleppo pine maintains itsphotosynthetic ability during hardening in contrast to otherconiferous species from colder climates. The effect of nitrogenfertilization on frost hardiness was small in comparison withchamber effect. Nitrogen fertilization slightly delayed theacquisition of hardening in the coldest chamber. Seedlings inthe warmest chamber did not become fully resistant to –5°C,but in the coldest chamber, where night temperature reached4°C, all the seedlings were resistant to the frost. Severedamage caused by frost could be related to a rapid rise of minimalfluorescence (F₀) but the best index of damage was the dropof F_v/F_m after freezing.     

Dieback Disease of Pines with Special Reference to Corsican Pine Pinus nigra var. Calabrica Schn.: II. The Relationship between Frost Resistance, Microclimate, and.Disease

The winter frost hardiness level of Corsican Pine buds in northernEngland is found to range from –16° C to –20C (+3° F to –4° F). Buds in the lower parts ofthe tree are more susceptible to frost damage than those nearerthe leader, and buds at a given position in the canopy on anorth-facing slope are less hardy than those in a correspondingposition on a tree in a south aspect. The symptoms produced by artificial freezing are distinct fromthose found in dieback disease, and temperature measurementsin diseased and healthy stands suggest that frosts are not severeenough to account for the disease. The lower hardiness levelsin disease susceptible areas are therefore considered to beof secondary importance and are probably a result of a weakenedphysiological condition in the tree. Diseased slopes are chiefly characterized by high humidities,low light intensities, and only slightly reduced air and soiltemperatures when compared with nearby healthy areas.     

Morphological and physiological differences in Scots pine seedlings of six seed origins

One-year-old seedlings of Scots pine (Pinus sylvestris L.) offour native seed origins (Loch Maree Islands, Glengarry/GlenMorriston, Glen Affric and Abernethy), a commercial Britishseedlot, and a seedlot from Hedesunda, in middle Sweden, werecompared at monthly intervals from October 1993 to April 1994.Seedling morphology, root condition, root frost hardiness andbud dry matter were determined at each date. There were clear morphological differences among seed origins.Seedlings raised from the commercial seedlot (A70) were largerbut had a poorer root:shoot ratio than the other seed origins.Of the native pines tested, the Loch Maree Islands origin allocateda larger proportion of its photosynthate to fine roots and needlesand smaller proportion to woody structures. Seedlings raised from the commercial British seedlot tendedto have poorer bud lignification than the other origins andalso, in autumn, higher electrolyte leakage rates from its fineroots. During winter, the Swedish origin had the lowest fineroot electrolyte leakage. Seedlings of all origins showed aprogressive increase in fine root hardiness towards mid-winterwith maximum hardiness (–7°C) in January. Dehardeningoccurred over subsequent months reaching –3°C in April.Differences among origins were evident. The Swedish seedlotdeveloped greater frost resistance than the other origins, hardeningbegan earlier in autumn and dehardening began later in spring.The commercial seedlot hardened later than the other originsbut reached a similar level of frost hardiness by January. Ofthe native pines, seedlings of the Loch Maree Islands originwere slowest to develop root hardiness.     

Coffee shade with Mimosa scabrella Benth. for frost protection in southern Brazil

Damage by radiative frosts is a major limiting factor for coffee cultivation in southern Brazil (south of 20° S latitude). The use of Mimosa scabrella (bracatinga) as a shade tree, to modify the local energy balance and thus prevent damage to the coffee plants, has been evaluated from 1986 to 1994. The study was carried out near Londrina, Parana State (23°23' S, 51°11′ W). During the experimental period, several radiative frosts with intensity ranging from moderate to very severe occurred at the site. Minimum coffee leaf temperatures during these events were 2 to 4 °C higher in the shaded plots. Due to frost protection, coffee bean yields on the average of 7 harvests were higher on the shaded plots. The potential of this system for frost protection in southern Brazil is discussed.     

Effect of Late-season Top-dressings of N (and K) applied to Conifer Transplants in the Nursery on their Survival and Growth on British Forest Sites

At two English forest nurseries, transplants of five coniferspecies—Picea sitchensis, Picea abies, Tsuga heterophylla,Abies grandis, and Pinus contorta—were grown with fertilizersupplying N, P, K, and Mg in amounts intended to be adequatefor producing healthy green trees with nutrient concentrationsin the ‘sufficiency range’ as determined by earlierexperiments. ‘Luxury uptake’ of nitrogen was obtainedwith top-dressings of ‘Nitro-Chalk’ applied in thenursery during early September, when top growth had nearly ceased.Tests of the effect of this extra N on forest establishmentwere repeated in four successive years under a wide range ofsoil and climatic conditions, keeping the trees in a cold storeduring each winter and planting them on forest sites in England,Scotland, or Wales during the following spring. Except for Grandfir, nitrogen advanced bud-break of all species during the firstsummer after planting and had no deleterious influence on survival.It tended to increase growth of Sitka spruce during the seasonafter planting, but in later years the differences became smallin relation to tree size. The effects on other species weresmall, except for one considerable decrease in the growth responseof Grand fir at a single site. Frost damage of Sitka spruce of Washington origin was severeon a Welsh and a Scottish site where this frost-sensitive provenancewould not normally be grown. At the Welsh, but not the Scottishsite, the nitrogen treatment increased the damage. In the few experiments (confined to Picea sitchensis) whichtested late-season potassium in the nursery, K concentrationswere increased from deficiency to barely sufficiency level;growth in the forest was increased in two of the four experiments.The extra K had no effect on frost damage.     

Shading Reduces both Visible and Invisible Frost Damage to Norway Spruce Seedlings in the Field

Two-year-old cuttings of Norway spruce were subjected to nightfrosts in spring on an exposed site in southern Sweden. Shadingwas used to assess the influence of sunlight on the extent ofdamage resulting from night frost. Chlorophyll fluorescencewas measured in needles in flushing shoots, and in shoots atthe stage of bud burst. The F_v:F_m ratio was significantly lowerfor plants exposed to light, compared with shaded plants onthe days following the night frost (minimum temperature –6°C).The effect was similar both in 1-year-old and current year needles.The low F_v:F_m ratios indicate damage to photosystem H, causedby an interaction between sub freezing temperatures and highlight intensity. Shading also increased the survival of flushingshoots. It is suggested that regeneration of Norway spruce onsites exposed to frost should be carried out in partial shade,for example under a shelterwood.     

Autumn frost damage: clonal variation in Sitka spruce

Current-year needles of Sitka spruce (Picea sitchensis) can be injured by frost after hardening of stem and buds in the autumn, and in Scotland trees of southern provenances are most susceptible to damage. In October 1993 a frost of −5°C caused needle damage to clonal plants of Alaskan, Queen Charlotte Islands, and Washington provenances of Sitka spruce that had been selected for variation in the onset of root dormancy. Visual damage assessment revealed significant differences among clones in the severity of damage, and this damage was positively correlated with the lateness of root dormancy. Measurement of electrolyte leakage from shoot samples confirmed the observed differences between clones. The large clonal differences in frost hardiness found in this study demonstrate a potential for selecting frost tolerant trees from within southern provenances of Sitka spruce.     

No difference in frost hardiness between high and low altitude Pinus sylvestris (L.) offspring

Seedlings representing offspring from 46 Swedish natural stands of Pinus sylvestris (L.) from latitude 63° to 68° and altitude 75 to 675 m were artifically frost hardened and tested for autumn frost hardiness using artifical freeze testing in a programmable freezing chamber. A clinal variation in frost hardiness was observed over latitude. Altitude had no effect on the frost hardiness. The results are discussed in relation to the reproduction‐ and migration biology of P. sylvestris.