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1.
vMDV感染雏鸡的Se-GSH-PX活性和LPO含量   总被引:2,自引:0,他引:2  
以马立克氏病强毒(vMDV)人工感染1日龄AA肉用雏鸡,于感染后7,14,28,42和56日龄分别检测雏鸡中枢、外周淋巴器官及主要内脏器官的含硒谷胱甘肽过氧化物酶(Se-GSH-PX)活性和脂质过氧化物(LPO)含量的变化。结果表明,vMDV感染雏鸡各器官Se-GSH-PX活性在检测各时期多显著低于健康对照雏鸡(P<0.05,P<0.01),而各器官LPO含量则多显著高于健康对照雏鸡(P<0.05,P<0.01),提示马立克氏病(MD)及其肿瘤的发生和发展与Se-GSH-PX抗氧化活性降低和LPO及其降解物对雏鸡的广泛病理损伤有关。  相似文献   

2.
半胱胺对小鹅血浆中β-END和某些激素的影响   总被引:33,自引:1,他引:32  
选用24只二月龄的杂交鹅(川白×太湖),随机分成对照组(12只)和试验组(12只)。试验组于日粮中一次性添加半胱胺(100mg/kg·BW)。处理后第三天从翅静脉采取血样。用RIA双抗法测定其中的β-END、IGF-I和各种激素的含量。结果表明:试验组的SS含量较对照组低19.64%(P<0.05)、而GH、IGF-I和β-END含量较对照组分别高50.00%(P<0.01)、62.80%(P<0.01)和44.55(P<0.05);与对照组相比较,试验组的TSH低32.32%(P<0.05),T4下降14.72%(P>0.05),而T3升高26.80%(P<0.01)。以上结果提示:半胱胺可能是通过降低鹅血液中SS含量,使β-END、IGF-I、GH和T3水平升高,从而促进了鹅的快速生长。  相似文献   

3.
牛超排技术的研究   总被引:7,自引:1,他引:6  
用4组药物组合:(1)PMSG+PGF2a;(2)PMSG+PGF2a+激光照射;(3)FSH+PGF2a;(4)FSH+Ctoprostenol,超排处理322头次供体牛。共采卵1663枚(头均5.81±4.68),可用胚胎980枚(头均3.43±3.94,占58.9%)。(3)、(4)两组胚胎可用率及可用胚胎数高于(1)、(2)组(p<0.05);黄牛胚胎可用率高于奶牛(p<0.01);育成牛胚胎可用率高于经产牛(p<0.01);供体牛前两次超排效果更好(p<0.05)。  相似文献   

4.
注射黄芪多糖雏鸡28日龄后,中枢、外周淋巴器官和主要实持性器官含硒谷胱甘肽过氧化物酶(Se-GSHPx)活性比健康对照雏鸡显著升高(P<0.05,P<0.01),1日龄雏鸡注射香菇多糖后Se-GSHPx活性呈现间断性跳跃式升高(P<0.05,P<0.01),两者比较香菇多糖应用初期生物效应优于黄芪多糖,而黄芪多糖应用后期生物效应则优于香菇多糖  相似文献   

5.
本文旨在探讨饲粮 n-6/n-3多不饱和脂肪酸(PUFA)比例对扬州鹅肌肉脂肪酸组成的影响。试验选取 160只健康、体重接近的 21日龄扬州鹅,随机分成 4组,分别饲喂 n-6/n-3PUFA比例为 12∶1、9∶1、6∶1及 3∶1的饲粮,于 70日龄时取胸肌和腿肌,进行脂肪酸组成的测定。结果表明:1)在胸肌中,亚油酸的含量 9∶1组显著高于 3∶1组(P<0.05),而与其他各组之间无显著差异(P>0.05);α-亚麻酸的含量 3∶1组显著高于 6∶1和 9∶1组(P<0.05),极显著高于12∶1组(P<0.01)。2)在腿肌中,亚油酸的含量 12∶1和 6∶1组均显著高于 3∶1组(P<0.05),而与9∶1组无显著差异(P>0.05);α-亚麻酸的含量 3∶1组显著高于 6∶1组(P<0.05),极显著高于12∶1和 9∶1组(P<0.01)。综上所述,鹅肌肉中 n-6/n-3PUFA比例随饲粮中该比例的下降而下降,并以 3∶1组最低,但除 12∶1组的腿肌 n-6/n-3PUFA比例较高外,其他各组比例均在世界卫生组织推荐的适于人体健康的(6∶1)~(10∶1)的比例范围内。  相似文献   

6.
断奶仔猪赖氨酸需求参数的研究   总被引:9,自引:1,他引:8  
试验选取体重约8千克的杜×大×长三元杂交断奶仔猪(公母各半)72头,随机分为6组,每组2个重复,每个重复6头猪,分别喂以含赖氨酸(Lys)水平为0.85,0.95,1.05,1.10,1.15,1.20%的饲粮。通过测定饲粮Lys水平对断奶仔猪生产性能和血清氮浓度的影响,探讨断奶仔猪Lys的需求参数。结果表明,随着饲粮中Lys水平的提高,仔猪的平均日增重增加,饲料转化率显著改善(P<0.05),并且在1.15%的Lys组生产性能达到最佳,进一步提高Lys水平,生产性能反而下降。各组采食量差异不显著(P>0.05)。当饲粮Lys水平由0.85%升至1.15%,血清氮浓度无显著差异(P>0.05),但1.2%组极显著地高于其他各组(P<0.01),反映该组猪对蛋白质利用较差,这与该组猪生产性能差相符。以上结论提示,8~19千克断奶仔猪Lys需求参数为饲粮的1.15%,按AEC(1989)有关饲料Lys可消化率数据,折算其相应真可消化和表观可消化Lys为1.032%和0.967%。  相似文献   

7.
采用三因素二次回归正交组合设计 ,每组两个重复 ,公母各半 ,研究了能量(DE :3.0~3.8Mcal/kg) ,粗蛋白质水平(CP:15 %~25 %)与赖氨酸/能量比(Lys:DE) :0.6~1.2g.Lys/MJ.DE对28日龄断奶仔猪(长白×汉梅)的日增重(ADG)、饲料转化率(F/G)、日采食量(ADFI)、血浆尿素氮(PUN)及血浆游离赖氨酸的影响 ,试验期42天。试验结果表明 :能量、蛋白质水平及赖氨酸/能量比对仔猪生产性能均有影响(P<0.01) ,其中以赖氨酸/能量比的作用最重要。仔猪28~60…  相似文献   

8.
香菇多糖和黄芪多糖对vAMV感染雏鸡Se—GSHPX活性的影响   总被引:4,自引:0,他引:4  
3日龄AA肉用雏鸡禽成髓细胞性白血病强毒(vAMV)感染并注射香菇多糖后,均可降低禽成髓细胞性白血病(AMB)的发病率和死亡率,显著增强中枢、外周淋巴器官和主要实质器官的含硒谷胱甘肽过氧化物酶(Se-GSHPX)活性(P<0.05,P<0.01)。香菇多糖的生物效应17日龄后显著增强(P<0.05,P<0.01),黄芪多糖的生物效应则全程均见显著增强(P<0.05,P<0.01)。  相似文献   

9.
口服半胱胺引起绵羊外周血液GH,IGF—1和SS激素的变化   总被引:16,自引:1,他引:15  
试验羊装置临时性颈静脉血管插管,采集血样,测定血液GH、IGF-1和SS。结果,6日内外周血液中血浆GH浓度升高56.2%(P<0.01),IGF-1浓度升高45.3%(P<0.01),SS浓度降低22.7%(P<0.01)。本试验证明半胱胺制成颗粒饲料,经口服可通过瘤胃进入小肠吸收,抑制体内SS水平,使外周血液中GH、IGF-1水平相应升高,同时胰岛素、T3、T4浓度也升高。结果提示半胱胺颗粒料经口服可促进反刍动物的生长。  相似文献   

10.
《中国畜牧杂志》1995,(4):11-13
本研究选用18头28±1日龄断奶的杂交仔猪(平均体重约5.5kg),研究低蛋白质氨基酸平衡饲粮对早期断奶仔猪腹泻和生产性能的影响。试验结果显示:(1)低蛋白质氨基酸平衡饲粮使仔猪血浆挥发性盐基氨含量、血浆尿素氨含量和腹泻指数降低(P<0.05或0.01)。(2)采食全植物蛋白型氨基酸平衡饲粮(CP18.3%)的仔猪,其平均日增重(ADG)、平均日采食量(ADFI)和料肉比(P/G)与采食复合蛋白型对照饲粮(CP19.7%)的仔猪差异不显著(P>0.05),但前者的增重成本比后者降低(P<0.05)31%。(3)采食复合蛋白型氨基酸平衡饲粮(CP18.4%)的仔猪的生产性能优于采食复合蛋白型对照饲粮(CP19.7%)的仔猪,前者的ADG和ADFI分别提高(P<0.05)61%和32%,F/G和增重成本分别下降(P<0.05)24%和37%。(4)采食全植物蛋白型氨基酸平衡饲粮的仔猪,其ADG和ADFI低于(P<0.01)采食复合蛋白型氨基酸平衡饲粮的仔猪。前者的F/G和增重成本趋于高于后者,但差异不显著(P>0.05)。以上结果表明,低蛋白质氨基酸平衡饲粮可显著降低仔猪断奶后腹泻和提高仔猪生产性能。复合蛋白型?  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

14.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

15.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

16.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

17.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

18.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

19.
The purpose of this study was to determine the frequency of different tumor types within a large cohort of cats with intracranial neoplasia and to attempt to correlate signalment, tumor size and location, and survival time for each tumor. Medical records of 160 cats with confirmed intracranial neoplasia evaluated between 1985 and 2001 were reviewed. Parameters evaluated included age, sex, breed, FeLV/FIV status, clinical signs, duration of signs, number of tumors, tumor location(s), imaging results, treatment, survival times, and histopathologic diagnosis. Most of the cats were older (11.3 +/- 3.8 years). Primary tumors accounted for 70.6% of cases. Metastasis and direct extension of secondary tumors accounted for only 5.6 and 3.8% of cases, respectively. Twelve cats (7.5%) had 2 or more discrete tumors of the same type, whereas 16 cats (10.0%) had 2 different types of intracranial tumors. The most common tumor types were meningioma (n = 93, 58.1%), lymphoma (n = 23, 14.4%), pituitary tumors (n = 14, 8.8%), and gliomas (n = 12, 7.5%). The most common neurological signs were altered consciousness (n = 42, 26.2%), circling (n = 36, 22.5%), and seizures (n = 36, 22.5%). Cats without specific neurological signs were common (n = 34, 21.2%). The tumor was considered an incidental finding in 30 (18.8%) cats. In addition to expected relationships (eg, meninges and meningioma, pituitary and pituitary tumors), we found that lesion location was predictive of tumor type with diffuse cerebral or brainstem involvement predictive of lymphoma and third ventricle involvement predictive of meningioma.  相似文献   

20.
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