Losses of nitrate-nitrogen in water draining from under autumn-sown crops established by direct drilling or mouldboard ploughing

The leaching of nitrate-N under autumn-sown arable crops was measured using hydro-logically isolated plots, about 0.24 ha in area, from 1984–1988. Fluxes of water and nitrate moving over the soil surface (surface runoff), at the interface between topsoil and subsoil (interflow), and in the subsoil (drainflow) were monitored in plots with mole-and-pipe drain systems (drained plots); surface runoff and interflow only were monitored in ‘undrained’ plots. Half the drained and undrained plots were direct-drilled, and on the other half seedbeds were prepared by tillage to 200 mm. Tillage increased the total leaching loss of nitrate by 21 % compared with direct drilling in drained plots. About 95% or the nitrate moving from the soil was present in the water intercepted by the subsoil drains in these plots. In undrained plots less water and nitrate were collected in total; more of the nitrate was present in interflow on ploughed plots and in surface runoff in direct-drilled land. Losses of nitrate for the whole experiment from 1978-1988 were analysed. This showed that, between the harvest of one crop and the spring application of fertilizer to the next, loss of nitrate-N from ploughed land (L_p) was approximated by L_p=22+Fkg N ha^?1, where F was the autumn fertilizer-N applied. After fertilizer was applied in spring, loss of nitrate-N depended on rainfall such that for 100 mm rainfall about 30% of the fertilizer-N was lost by leaching. About 18% more nitrate-N was lost from direct-drilled land than from ploughed land in spring, but the total loss was generally small compared to that over winter. The apparent net mineralization of organic-N was measured in 1988. In autumn and winter there was little effect of tillage treatment (26 and 31 kg N ha^?1 on direct drilled and tilled plots respectively). However, over the year 83 kg N ha^?1 were mineralized in tilled plots, and 67 kg N ha^?1 in direct-drilled plots. Five factors governing the leaching of nitrate are assessed and this identified that fertilizer nitrogen application to the seedbed of winter sown crops and the mineralization of nitrogen from the residues of the previous crop are the most significant factors for nitrogen leaching in the UK.     

Denitrification loss from a grassland soil in the field receiving different rates of nitrogen as ammonium nitrate

Denitrification loss from a loam under a cut ryegrass sward receiving 0, 250 and 500 kg N ha^?1 a^?1 in four equal amounts was measured during 14 months using the acetylene-inhibition technique. The rate of denitrification responded rapidly to changes in soil water content as affected by rain. Mean rates of denitrification exceeded 0.2 kg N ha^?1 day^?1 only when the soil water content was >20% (w/w) and nitrate was >5μ N g^?1 in the upper 20 cm of the profile and when soil temperature at 2 cm was >5–8°C. When the soil dried to a water content <20%, denitrification decreased to <0.05 kg N ha^?1 day^?1. Highest rates (up to 2.0 kg N ha^?1 day^?1) were observed following application of fertilizer to soil at a water content of about 30% (w/w) in early spring. Denitrification in the control plot during this period was generally about a hundredth of that in plots treated with ammonium nitrate. High rates of N₂O loss (up to 0.30 kg N ha^?1 day-1) were invariably associated with high rates of denitrification (> 0.2 kg N ha^?1 day^?1). However, within 2–3 weeks following application of fertilizer to the plot receiving 250 kg N ha^?1 a^?1 the soil acted as a sink for atmospheric N₂O when its water content was >20% and its temperature >5–8°C. Annual N losses arising from denitrification were 1.6, 11.1 and 29.1 kg N ha^?1 for the plots receiving 0, 250 and 500 kg N ha^?1 a^?1, respectively. More than 60% of the annual loss occurred during a period of 8 weeks when fertilizer was applied to soil with a water content >20%.     

Nitrate leaching loss under annual and perennial pastures with and without lime on a duplex (texture contrast) soil in humid southeastern Australia

Mineral N accumulates in autumn under pastures in southeastern Australia and is at risk of leaching as nitrate during winter. Nitrate leaching loss and soil mineral N concentrations were measured under pastures grazed by sheep on a duplex (texture contrast) soil in southern New South Wales from 1994 to 1996. Legume (Trifolium subterraneum)‐based pastures contained either annual grass (Lolium rigidum) or perennial grasses (Phalaris aquatica and Dactylis glomerata), and had a control (soil pH 4.1 in 0.01 m CaCl₂) or lime treatment (pH 5.5). One of the four replicates was monitored for surface runoff and subsurface flow (the top of the B horizon), and solution NO₃^– concentrations. The soil contained more mineral N in autumn (64–133 kg N ha^?1 to 120 cm) than in spring (51–96 kg N ha^?1), with NO₃^– comprising 70–77%. No NO₃^– leached in 1994 (475 mm rainfall). In 1995 (697 mm rainfall) and 1996 (666 mm rainfall), the solution at 20 cm depth and subsurface flow contained 20–50 mg N l^?1 as NO₃^– initially but < 1 mg N l^?1 by spring. Nitrate‐N concentrations at 120 cm ranged between 2 and 22 mg N l^?1 during winter. Losses of NO₃^– were small in surface runoff (0–2 kg N ha^?1 year^?1). In 1995, 9–19 kg N ha^?1 was lost in subsurface flow. Deep drainage losses were 3–12 kg N ha^?1 in 1995 and 4–10 kg N ha^?1 in 1996, with the most loss occurring under limed annual pasture. Averaged over 3 years, N losses were 9 and 15 kg N ha^?1 year^?1 under control and limed annual pastures, respectively, and 6 and 8 kg N ha^?1 year^?1 under control and limed perennial pastures. Nitrate losses in the wet year of 1995 were 22, 33, 13 and 19 kg N ha^?1 under the four respective pastures. The increased loss of N caused by liming was of a similar amount to the decreased N loss by maintaining perennial pasture as distinct from an annual pasture.     

Subsoil retention of organic and inorganic nitrogen in a Brazilian savanna Oxisol

Abstract. Nitrogen (N) loss by leaching poses great challenges for N availability to crops as well as nitrate pollution of groundwater. Few studies address this issue with respect to the role of the subsoil in the deep and highly weathered savanna soils of the tropics, which exhibit different adsorption and drainage patterns to soils in temperate environments. In an Anionic Acrustox of the Brazilian savanna, the Cerrado, dynamics and budgets of applied N were studied in organic and inorganic soil pools of two maize (Zea mays L.) – soybean (Glycine max (L.) Merr.) rotations using ¹⁵N tracing. Labelled ammonium sulphate was applied at 10 kg N ha^?1 (with 10 atom%¹⁵N excess) to both maize and soybean at the beginning of the cropping season. Amounts and isotopic composition of N were determined in above‐ground biomass, soil, adsorbed mineral N, and in soil solution at 0.15, 0.3, 0.8, 1.2 and 2 m depths using suction lysimeters throughout one cropping season. The applied ammonium was rapidly nitrified or immobilized in soil organic matter, and recovery of applied ammonium in soil 2 weeks after application was negligible. Large amounts of nitrate were adsorbed in the subsoil (150–300 kg NO₃^?‐N ha^?1 per 2 m) matching total N uptake by the crops (130–400 kg N ha^?1). Throughout one cropping season, more applied N (49–77%; determined by ¹⁵N tracers) was immobilized in soil organic matter than was present as adsorbed nitrate (2–3%). Most of the applied N (71–96% of ¹⁵N recovery) was found in the subsoil at 0.15–2 m depth. This coincided with an increase with depth of dissolved organic N as a proportion of total dissolved N (39–63%). Hydrophilic organic N was the dominant fraction of dissolved organic N and was, together with nitrate, the most important carrier for applied N. Most of this N (>80%) was leached from the topsoil (0–0.15 m) during the first 30 days after application. Subsoil N retention as both adsorbed inorganic N, and especially soil organic N, was found to be of great importance in determining N losses, soil N depletion and the potential of nitrate contamination of groundwater.     

Modeling of Interactive Effects of Rainfall,Evaporation, Soil Temperature,and Soil Fertility for Sustainable Productivity of Sorghum + Cowpea and Cotton + Black Gram Intercrops under Rotation Trials in a Rain-Fed Semi-arid Vertisol

Long-term effects of the different combinations of nutrient-management treatments were studied on crop yields of sorghum + cowpea in rotation with cotton + black gram. The effects of rainfall, soil temperature, and evaporation on the status of soil fertility and productivity of crops were also modeled and evaluated using a multivariate regression technique. The study was conducted on a permanent experimental site of rain-fed semi-arid Vertisol at the All-India Coordinated Research Project on Dryland Agriculture, Kovilpatti Centre, India, during 1995 to 2007 using 13 combinations of nutrient-management treatments. Application of 20 kg nitrogen (N) (urea) + 20 kg N [farmyard manure (FYM)] + 20 kg phosphorus (P) ha^?1 gave the greatest mean grain yield (2146 kg ha^?1) of sorghum and the fourth greatest mean yield (76 kg ha^?1) of cowpea under sorghum + cowpea system. The same treatment maintained the greatest mean yield of cotton (546 kg ha^?1) and black gram (236 kg ha^?1) under a cotton + cowpea system. When soil fertility was monitored, this treatment maintained the greatest mean soil organic carbon (4.4 g kg^?1), available soil P (10.9 kg ha^?1), and available soil potassium (K) (411 kg ha^?1), and the second greatest level of mean available soil N (135 kg ha^?1) after the 13-year study. The treatments differed significantly from each other in influencing soil organic carbon (C); available soil N, P, and K; and yield of crops attained under sorghum + cowpea and cotton + black gram rotations. Soil temperature at different soil depths at 07:20 h and rainfall had a significant influence on the status of soil organic C. Based on the prediction models developed between long-term yield and soil fertility variables, 20 kg N (urea) + 20 kg N (FYM) + 20 kg P ha^?1 could be prescribed for sorghum + cowpea, and 20 kg N (urea) + 20 kg N (FYM) could be prescribed for cotton + black gram. These combinations of treatments would provide a sustainable yield in the range of 1681 to 2146 kg ha^?1 of sorghum, 74 to 76 kg ha^?1 of cowpea, 486 to 546 kg ha^?1 of cotton, and 180 to 236 kg ha^?1 of black gram over the years. Beside assuring greater yields, these soil and nutrient management options would also help in maintaining maximum soil organic C of 3.8 to 4.4 g kg^?1 soil, available N of 126 to 135 kg ha^?1, available soil P of 8.9 to 10.9 kg ha^?1, and available soil K of 392 to 411 kg ha^?1 over the years. These prediction models for crop yields and fertility status can help us to understand the quantitative relationships between crop yields and nutrients status in soil. Because black gram is unsustainable, as an alternative, sorghum + cowpea could be rotated with cotton for attaining maximum productivity, assuring sustainability, and maintaining soil fertility on rain-fed semi-arid Vertisol soils.     

Estimation of the total gaseous nitrogen losses from clay soils under laboratory and field conditions

Acetylene blockage was evaluated as a method for measuring losses of N₂O + N₂ from two Denchworth series clay soils. The denitrification potential in anaerobic, dark incubations at 20°C with nitrate (equivalent to 100 kg N ha^?1 0–20 cm depth), maximum water holding capacity, and acetylene (1%), was equivalent to 32 ± 11 and 39 ± 6 kg N ha^?1 per day for the two 0–20 cm soils and was positively correlated with carbon content (r= 0.98). After 4 days N₂O was reduced to N₂ in the presence of C₂H₂. In April 1980 following irrigation (24 mm) and applications of ammonium nitrate (70 kg N ha^?1) and acetylene, the mean nitrous oxide flux from soil under permanent grass was 0.05 ± 0.01 kg N₂O-N ha^?1 per day for 8 days. In June 1980, the losses of nitrogen from cultivated soils under winter wheat after irrigation (36 mm) and acetylene treatment were 0.006 ± 0.002 and 0.04–0.07 ± 0.01 kg N ha^?1 per day respectively before and after fertilizer application (70 kg N ha^?1). The nitrous oxide flux in the presence of acetylene decreased briefly, indicating that nitrification was rate determining in drying soil.     

Simulation des Nitrataustrags im Winterhalbjahr aus Intensiv genutzten Ackerböden in der Geest

Modeling nitrate leaching during the winter halfyear from sandy arable soils under intensive cultivation Three years (1989–91) of post harvest and winter nitrogen dynamics (August to March) were simulated in 20 arable sandy soils to quantify nitrate leaching during winter time. Easily accessible soil, weather and management data were used for a simple but deterministic model. The calculated mineral N (N_min) content and distribution in the soil (0–90 cm) were compared to more than 100 measurements from September to March each season. An overall agreement of approximately 50% between measured and simulated N_min values was obtained. The simulation over- or underestimated the measured N_min depending on the rainfall and temperature distribution which varied from year to year. Practically, the effect of fertilizer application was largely (60%) responsible for deviations greater than ±20 kgN ha^?1 from the 1:1-line. Ignoring these instances, 80% of the simulated N_min contents were within these “confidence limits” of ±20 kgN ha^?1. Considering the nitrogen distribution in the profile, the N_min content is underestimated in the top soil, but overestimated in the subsoil. Based on the 95% confidence intervals (measured versus simulated) the estimate was better for the lower (30–90 cm) than for the upper part of the profile (0–30 cm). It is concluded that winter leaching can be reduced from 130 kgN ha^?1 (corn, winter grain) to about 10 kgN ha^?1 growing winter hard forage crops. Two major processes were identified as reasons for the disagreement and are proposed for further model improvement: (1) The simulation underestimates the short term transport velocity on the basis of field capacity derived from survey data. (2) Nitrogen is mineralized quickly in sandy soils, especially after catch crops, and sometimes due to freeze-thaw effects. Furthermore, as ammonium remains in the surface, nitrification needs to be explicitly simulated.     

Effect of plantain on nitrous oxide emissions and soil nitrification rate in pasture soil under a simulated urine patch in Canterbury,New Zealand

Purpose

The aim of this research was to quantify the effect of plantain (Plantago lanceolata L.) on soil nitrification rate, functional gene abundance of soil ammonia oxidisers, and the concomitant effect on nitrous oxide emissions from urine patches in a shallow, free-draining soil in Canterbury during late autumn/winter season.

Materials and methods

Urine was collected from dairy cows grazing either ryegrass/white clover (RGWC), 30% plantain (P30) mixed in with RGWC or 100% plantain (P100) pasture, and applied at two rates (700 or 450 kg N ha^?1) to intact soil blocks growing either RGWC, P30 or P100 pasture.

Results and discussion

Results showed that increased plantain content reduced N-concentration in urine from 7.2 in RGWC urine to 4.5 and 3.7 g N L^?1 in P30 and P100 urine, respectively. Total N₂O emissions and emission factors (EF₃) from urine-treated pastures were low, <?2 kg N ha^?1 and <?0.22%, respectively. Urine application at the lower urine N-loading rate of 450 kg N ha^?1 (i.e. representative of that in a P30 urine patch) resulted in 30% lower N₂O emissions (P?<?0.01) and 35% lower soil nitrate concentrations (P?<?0.001) compared to those at the higher urine loading rate of 700 kg N ha^?1 (i.e. representative of that in a RGWC urine patch). Increasing plantain content in the pasture sward from 0 to 30% and 100% with urine N applied at the same loading rate did not reduce N₂O emissions or nitrification compared to the standard ryegrass-white clover pasture. Cow urine derived from the different pasture diets had no effect on N₂O emissions, N transformation or ammonia-oxidiser abundance in soil compared to the RGWC urine applied at the same rate.

Conclusions

The main effect of plantain in this study appears to be related to the reduction in urine N-loading rate, rather than factors related to urine properties or plantain-soil interactions.

     

Seasonal soil nitrogen dynamics affect yields of lowland rice in the savanna zone of West Africa

Background : Rice production in low‐input systems of West Africa relies largely on nitrogen supply from the soil. Especially in the dry savanna agro‐ecological zone, soil organic N is mineralized during the transition period between the dry and the wet seasons. In addition, in the inland valley landscape, soil N that is mineralized on slopes may be translocated as nitrate into the lowlands. There, both in‐situ mineralized as well as the laterally translocated nitrate‐N will be exposed to anaerobic conditions and is thus prone to losses. Aim : We determined the dynamics of soil NO₃‐N along a valley toposequence during the dry‐to‐wet season transition period and the effects of soil N‐conserving production strategies on the grain yield of rainfed lowland rice grown during the subsequent wet season. Methods : Field experiments in Dano (Burkina Faso) assessed during two consecutive years the temporal dynamics and spatial fluxes of soil nitrate along a toposequence. We applied sequential and depth‐stratified soil nitrate analysis and nitrate absorption in ion exchange resin capsules in lowlands that were open to subsurface interflow and in those where the interflow from the was intercepted. During one year only we also assessed the effect of pre‐rice vegetation on conserving this NO₃‐N as well as on N addition by biological N₂ fixation in legumes using δ¹⁵N isotope dilution. Finally, we determined the impact of soil N fluxes and their differential management during the transition season on growth, yield and N use of rainfed lowland rice. Results : Following the first rainfall event of the season, soil NO₃‐N initially accumulated and subsequently decreased gradually in the soil of the valley slope. Much of this nitrate N was translocated by lateral sub‐surface flow into the valley bottom wetland. There, pre‐rice vegetation was able to absorb much of the in‐situ mineralized and the laterally‐translocated soil NO₃‐N, reducing its accumulation in the soil from 40–43 kg N ha^?1 under a bare fallow to 1–23 kg N ha^?1 in soils covered by vegetation. Nitrogen accumulation in the biomass of the transition season crops ranged from 44 to 79 kg N ha^?1 with a 36–39% contribution from biological N₂ fixation in the case of legumes. Rice agronomic performance improved following the incorporation as green manure of this “nitrate catching” vegetation, with yields increasing up to 3.5 t ha^?1 with N₂‐fixing transition seasons crops. Conclusion : Thus, integrating transition season legumes during the pre‐rice cropping niche in the prevailing low‐input systems in inland valleys of the dry savanna zone of West Africa can temporarily conserve substantial amounts of soil NO₃‐N. It can also add biologically‐fixed N, thus contributing to increase rice yields in the short‐term and, in the long‐term, possibly maintaining or improving soil fertility in the lowland.     

Effect of cropping systems on nitrogen mineralization in soils

 Understanding the effect of cropping systems on N mineralization in soils is crucial for a better assessment of N fertilizer requirements of crops in order to minimize nitrate contamination of surface and groundwater resources. The effects of crop rotations and N fertilization on N mineralization were studied in soils from two long-term field experiments at the Northeast Research Center and the Clarion-Webster Research Center in Iowa that were initiated in 1979 and 1954, respectively. Surface soil samples were taken in 1996 from plots of corn (Zea mays L.), soybean (Glycine max (L.) Merr.), oats (Avena sativa L.), or meadow (alfalfa) (Medicago sativa L.) that had received 0 or 180 kg N ha^–1 before corn and an annual application of 20 kg P and 56 kg K ha^–1. N mineralization was studied in leaching columns under aerobic conditions at 30  °C for 24 weeks. The results showed that N mineralization was affected by cover crop at the time of sampling. Continuous soybean decreased, whereas inclusion of meadow increased, the amount of cumulative N mineralized. The mineralizable N pool (N _o) varied considerably among the soil samples studied, ranging from 137 mg N kg^–1 soil under continuous soybean to >500 mg N kg^–1 soil under meadow-based rotations, sampled in meadow. The results suggest that the N _o and/or organic N in soils under meadow-based cropping systems contained a higher proportion of active N fractions. Received: 10 February 1999     

A comparison of a pasture ley with a maize monoculture on the soil fertility and nutrient release in the succeeding crop

Specialization within agriculture has been a key factor in increasing farm income. The production systems have become increasingly simple, since farmers only grow a small number of crops which have a favourable market price. However, monocultural systems require increasing use of agrochemicals leading to unsustainable environmental costs. In this work, the soil fertility of two plots in a crop rotation previously grown for 5 years as pasture or maize monoculture was evaluated. In the pasture, the upper 0–20 cm soil layer sequestered 17.4 Mg organic C ha^?1 and accumulated 403 kg N ha^?1 more than under maize monoculture. Analytical data from pot experiments showed that soil samples from the pasture plot released significantly more mineral N than soil samples from the maize monoculture. Maize dry matter (DM) yields in 2012 and 2013 were 15.3 and 10.0 Mg ha^?1 in the pasture plot and 8.8 and 8.4 Mg ha^?1 in the maize monoculture plot. Nitrogen recoveries by maize were 175.4 and 68.0 kg ha^?1 in the pasture and 78.3 and 50.3 kg ha^?1 in the maize monoculture plot. The pool of organic matter accumulated during the pasture phase immobilized important nutrients which benefited the succeeding crop as the organic substrate was mineralized.     

Mineral N dynamics in bare and cropped Leucaena leucocephala and Dactyladenia barteri alley cropping systems after the addition of 15N-labelled leaf residues

In tropical cropping systems with few external inputs, efficient management of mineral N derived from added organic residues is essential for the proper functioning of the system. We studied the dynamics of mineral nitrogen (N) in the top 100 cm of soil with a system of tensiometers and suction cups after applying ¹⁵N-labelled Leucaena leucocephala and Dactyladenia barteri residues to bare and cropped microplots installed in the respective alley cropping systems, and followed the fate of the N for two maize-cowpea rotations (1992 and 1993). Fifty days after applying the residues (DDA), 20% of the added residue N was found in the soil profile of the bare Leucaena treatment, and 5% under Dactyladenia, compared with 5% and 1%, respectively, where cropped. All values decreased to about 1% after 505 days. In the cropped soil, no mineral N derived from the residues was lost by leaching during the first 6 weeks. As the maize grew, the soil profile was gradually depleted of nitrate to near Zero in the Dactyladenia treatment, whereas during the cowpea season the amount of nitrate N increased to 36 kg N ha^?1 for the Leucaena treatment, and 26 kg N ha^?1 for the Dactyladenia treatment. The soil of the bare microplots contained substantially more nitrate N (98 and 47 kg N ha^-1 during the first year on average, under Leucaena and Dactyladenia, respetively) than that of the cropped microplots, except during the 1993 cowpea season. Nitrate residing in the subsoil (80–100 cm) in the bare treatments was not readily leached to deeper soil. The risk of losses of native mineral N was greatest during the first 50 DAA and to a lesser extent during the cowpea seasons. Improved management of the hedgerows could increase the potential of the hedgerow trees to recycle mineral N.     

Denitrification losses from 15N-labelled calcium nitrate fertilizer in a clay soil in the field

Calcium nitrate fertilizer containing 92.3 atoms % excess nitrogen-15 was applied on 5 May 1981 at a rate equivalent to 100 kg N ha^?1 to a clay soil in southern England cropped to winter wheat. Samples of the soil gases were collected frequently during the following 3 weeks. The soil oxygen concentration declined to 5% after 60 mm rain. A maximum of 1.5 ± 0.5 atom % N-15 enrichment in labelled N₂ gas (²⁹N₂) was detected in the soil atmosphere on 28 May. Total denitrification losses, calculated from air-filled pore space and rates of gas loss from the soil estimated using a Fick's law approximation, were 9.5 kg N ha^?1 with a daily rate of 0.30 ± 0.07 kg N ha^?1. Estimated total losses were greater than 30 kg N ha^?1, 93% in the form N₂, but the estimation depends on several assumptions about the amount of double labelled gas (³⁰N₂), rates of gas diffusion and flux.     

Using time domain reflectometry for monitoring mineralization of nitrogen from soil organic matter

The mineralization of nitrogen from soil organic matter is important when one tries to optimize nitrogen fertilization and assess risks of N losses to the environment, but its measurement is laborious and expensive. We have explored the possibilities for monitoring N mineralization directly using time domain reflectometry (TDR). Net N and S mineralization were monitored over a 101‐day period in two layers (0–30 and 30–60 cm) of a loamy sand soil during aerobic incubation in a laboratory experiment. At the same time electrical conductivity of the bulk soil, σ_a, was measured by TDR. A series of calibration measurements with different amounts of KNO₃ at different soil moisture contents was made with the topsoil to calculate the electrical conductivity, σ_w, of the soil solution from σ_a and θ. The actual σ_w was determined from the conductivity of 1:2 soil:water extracts (σ_1:2) with a mass balance approach using measured NO₃^– concentrations, after correction for ions present prior to the addition of KNO₃. The average N mineralization rate in the topsoil was small (0.12 mg N kg^?1 day^?1), and, as expected, very small in the subsoil (0.023 mg N kg^?1 day^?1). In the top layer NO₃^– concentrations calculated from σ_a determined by TDR slightly underestimated measured concentrations in the first 4 weeks, and in the second half of the incubation there was a significant overestimation of measured NO₃^–. Using the sum of both measured NO₃^– and SO₄^2– reduced the overestimation. In the subsoil calculated NO₃^– concentrations strongly and consistently overestimated measured concentrations, although both followed the same trend. As S mineralization in the subsoil was very small, and initial SO₄^2– concentrations were largely taken into account in the calibration relations, SO₄^2– concentrations could not explain the overestimation. The very small NO₃^– and SO₄^2– concentrations in the B layer, at the lower limit of the concentrations used in the calibrations, are a possible explanation for the discrepancies. A separate calibration for the subsoil could also be required to improve estimates of NO₃^– concentrations.     

Phosphorus fractions in bulk subsoil and its biopore systems

Improving phosphorus (P) accessibility in subsoils could be a key factor for sustainable crop management. This study aims to explain the quantity of different P fractions in subsoil and its biopore systems, and to test the hypothesis that crops with either fibrous (fescue) or tap‐root systems (lucerne and chicory) leave behind a characteristic P pattern in bulk subsoil, biopore linings and the rhizosphere. The crops were cultivated for up to 3 years in a randomized field experiment on a Haplic Luvisol developed from loess. Aqua regia‐extractable P (referred to as total P) and calcium acetate lactate‐extractable P (P_CAL) were assessed at 0–30 (Ap horizon), 30–45 (E/B horizon), 45–75 and 75–105 cm subsoil depths. In addition, sequential P fractionation was performed on different soil compartments between 45 and 75 cm depths. The results showed that total P stocks below the Ap horizon (30–105 cm) amounted to 5.6 t ha^?1, which was twice as large as in the Ap, although the Ap contained larger portions of P_CAL. Both P_CAL and sequential P extractions showed that biopore linings and the rhizosphere at the 45–75 cm depth were enriched, rather than depleted, in P. The content of inorganic P (81–90% of total P) increased in the following order: bulk soil = biopores _{<2 mm} ≤ rhizosphere ≤ biopores _{>2 mm}. Biopores _{>2 mm} and rhizosphere soil were clearly enriched in resin‐ and NaHCO₃‐extractable P_i and P_o fractions. However, we failed to attribute these P distribution patterns to different crops, suggesting that major properties of biopore P originated from relict biopores, rather than being influenced by recent root systems. The stocks of the sum of these P fractions in the bulk subsoil (182 kg ha^?1 at 45–75 cm depth) far exceeded those in the biopores (3.7 kg ha^?1 in biopores _{>2 mm} and 0.2 kg ha^?1 in biopores _{<2 mm}). Hence, these biopores may form attractive locations for root growth into the subsoil but are unlikely to sustain overall plant nutrition.     

Changes in the soil C and N contents,C decomposition and N mineralization potentials in a rice paddy after long-term application of inorganic fertilizers and organic matter

A long-term experiment on combined inorganic fertilizers and organic matter in paddy rice (Oryza sativa L.) cultivation began in May 1982 in Yamagata, northeastern Japan. In 2012, after the 31^st harvest, soil samples were collected from five fertilizer treatments [(1) PK, (2) NPK, (3) NPK + 6 Mg ha^?1 rice straw (RS), (4) NPK + 10 Mg ha^?1 rice straw compost (CM1), and (5) NPK + 30 Mg ha^?1 rice straw compost (CM3)], at five soil depths (0–5, 5–10, 10–15, 15–20 and 20–25 cm), to assess the changes in soil organic carbon (SOC) content and carbon (C) decomposition potential, total nitrogen (TN) content and nitrogen (N) mineralization potential resulting from long-term organic matter addition. The C decomposition potential was assessed based on the methane (CH₄) and carbon dioxide (CO₂) produced, while the N mineralization potential was determined from the potassium chloride (KCl)-extractable ammonium-nitrogen (NH₄⁺-N), after 2, 4, 6 and 8 weeks of anaerobic incubation at 30°C in the laboratory. Compared to NPK treatment, SOC in the total 0–25 cm layer increased by 67.3, 21.0 and10.8%, and TN increased by 64.2, 19.7 and 10.6%, in CM3, RS and CM1, respectively, and SOC and TN showed a slight reduction in the PK treatment by 5.2 and 5.7%, respectively. Applying rice straw compost (10 Mg ha^?1) instead of rice straw (6 Mg ha^?1) to rice paddies reduced methane production by about 19% after the soils were measured under 8 weeks of anaerobic incubation at 30°C. Soil carbon decomposition potential (Co) and nitrogen mineralization potential (No) were highly correlated with the SOC and TN contents. The mean ratio of Co/No was 4.49, lower than the mean ratio of SOC/TN (13.49) for all treatments, which indicated that the easily decomposed organic matter was from soil microbial biomass and soil proteins.     

Nitrous oxide emission from a grassland soil fertilized with slurry and calcium nitrate

Concentrations of nitrous oxide (N₂O) and oxygen were monitored over a 2-yr period in an imperfectly drained grassland soil receiving applications of N as cattle slurry or Ca(NO₃)₂. In both years N₂O concentrations in the different treatments were in the order nitrate > slurry > control. Gaseous diffusion coefficients were determined in soil cores by a krypton-85 tracer method and used to calculate approximate N₂O fluxes from the soil. Only 1–5 kg N ha^?1 was lost as N₂O after a single application of > 1200 kg N ha ^?1 as slurry compared with 3–11 kg N ha ^?1 lost after 100 kg was added as NO₃^?. Total gaseous losses (N₂O+N₂) could be expected to be higher in both cases.     

Nitrogen accumulation efficiency: Relationship between excess fertilizer and soil‐plant biological activity in winter wheat

The point at which nitrogen (N) applied approaches 100% recovery in the soil once plant and microbial sinks have been saturated has not been determined in winter wheat (Triticum aestivum L.) production systems. In dryland winter wheat, subsoil accumulation has not been found to occur until N rates exceed that required for maximum yield. Many conventional N rate experiments have not properly evaluated subsoil N accumulation due to the lack of equally spaced N rates at the high end of the spectrum over which accumulation is expected to occur. Therefore, the objectives of this study were to (i) determine when soil profile accumulation efficiencies reach 100% in continuous winter wheat production and (ii) to evaluate the potential for nitrate‐nitrogen (NO₃ ^‐N) leaching in continuous winter wheat when extremely high rates of fertilizer N are used. Two field experiments (T505 and T222) were conducted for two years using ten N rates (preplant‐incorporated) ranging from 0 to 5376 kg N ha¹. No additional preplant fertilizer was applied in the second year. Following the first and second year wheat harvest, soil cores were taken to 2.4 m and bulk density, ammonium‐nitrogen (NH₄‐N) and NO₃‐N were determined. Crop N‐use efficiency (NUE) (N uptake treated ‐ N uptake check/rate applied) and soil profile inorganic N accumulation efficiencies (NAE) [net inorganic N accumulation in the soil profile/(fertilizer applied ‐ net N removed in the crop)] changed with fertilizer rate and were inversely related. Priming (increased net mineralization of organic N pools when low rates of fertilizer N are applied) may have occurred since increased NUE was observed at low N rates. The highest N‐accumulation efficiencies were at N rates of 168 and 448 kg ha^‐1 in experiments T505 and T222, respectively. At both T222 and T505, no subsoil accumulation of NH₄‐N or NO₃‐N beyond 100 cm was observed for any of the N treatments when compared to the 0‐N check, even when N rates exceeded 448 kg ha^‐1.     

A lysimeter study of the fate of fertilizer nitrogen in spring barley crops grown on shallow soil overlying Chalk: crop uptake and leaching losses

The objective of this work was to determine the fate of fertilizer nitrogen (labelled with nitrogen-15) applied to an undisturbed shallow soil overlying Chalk contained in 10 lysimeters (80 cm diameter, 135 cm deep). Measurements are reported of the nitrogen uptake by four spring barley crops and the rate and extent of leaching of nitrate beyond the roots. The crops were fertilized with 0, 80 or 120 kg N ha^?1 in each of four years, but only the first application in 1977 was labelled with nitrogen ?15. Rainfall and irrigation approximated to the long-term average, but in two treatments dry or wet spring conditions were imposed for the 10 weeks after sowing the first crop in 1977. The dry matter and grain yields of the spring barley crops varied from year to year in the ranges 8.7–14.0 t ha^?1 and 3.5–6.1 t ha^?1 respectively. The total nitrogen harvested in the crop approximated to the amount of nitrogen applied in each year with an apparent recovery of fertilizer in the range 38–76%. The recovery of nitrogen derived from fertilizer (labelled with nitrogen-15) was 46–54% in the first crop and after 2 years rapidly declined to below 1%. The total amount of nitrogen-15 labelled fertilizer recovered in four barley crops was 49–57% of that applied. Mean annual nitrate concentrations in water draining from the base of the lysimeters were in the range 11.8–26.7 mg N 1^?1 and did not differ significantly between nitrogen fertilizer treatments (0, 80 and 120 kg N ha^?1 a^?1). In all treatments nitrate concentrations varied considerably within each growing season, with a cycle of peaks and troughs. Annual losses of nitrate were in the range 39–128 kg N ha^?1, and the mean annual losses over the 4 years varied between lysimeters from 65 to 83 kg N ha^?1. Nitrogen-15 labelled nitrate was detected in the first drainage water collected in autumn following its spring application, 5 months earlier. Recovery of fertilizer-derived nitrogen in drainage water was greatest during the winter following the second barley crop, and was 3.4–3.7% of the nitrogen-15 applied. Over the 4 years of the experiment 6.3–6.6% of labelled fertilizer was accounted for in drainage water, representing 2–3% of the total nitrogen lost by leaching.     

Denitrification Rate and Controlling Factors for Accumulated Nitrate in the Deep Subsoil of Intensive Farmlands: A Case Study in the North China Plain

Denitrification in subsoil(to a depth of 12 m) is an important mechanism to reduce nitrate(NO_3~-) leaching into groundwater.However, regulating mechanisms of subsoil denitrification, especially those in the deep subsoil beneath the crop root zone, have not been well documented. In this study, soil columns of 0–12 m depth were collected from intensively farmed fields in the North China Plain. The fields had received long-term nitrogen(N) fertilizer inputs at 0(N0), 200(N200) and 600(N600) kg N ha~(-1) year~(-1). Main soil properties related to denitrification, i.e., soil water content, NO_3~-, dissolved organic carbon(DOC), soil organic carbon(SOC),pH, denitrifying enzyme activity(DEA), and anaerobic denitrification rate(ADR), were determined. Statistical comparisons among the treatments were performed. The results showed that NO_3~- was more heavily accumulated in the entire soil profile of the N600 treatment, compared to the N0 and N200 treatments. The SOC, DOC, and ADR decreased with increasing soil depth in all treatments,whereas considerable DEA was observed throughout the subsoil. The long-term fertilizer rates affected ADR only in the upper 4 m soil layers. The ADRs in the N200 and N600 treatments were significantly correlated with DOC. Multiple regression analysis indicated that DOC rather than DEA was the key factor regulating denitrification beneath the root zone. Additional research is required to determine if carbon addition into subsoil can be a promising approach to enhance NO_3~- denitrification in the subsoil and consequently to mitigate groundwater NO_3~- contamination in the intensive farmlands.