Feeding activity and egg formation in hens lit continuously

1. The feeding patterns of five laying hens were examined in Skinner boxes under conditions of continuous light.

2. Four of the birds maintained a circadian rhythm, in two the rhythm was 24 h while in the other two it was slightly in excess of 24 h.

3. In all birds, feeding activity was less in the 12 h before oviposition than in the 12 h after.

4. When only the first egg of a clutch was considered, it was found that a low level of feeding activity was associated with the release of luteinising hormone (32 h before oviposition), there was then a sharp increase at ovulation to a new level which was maintained from about 23 to 18 h before oviposition and then a decline in feeding occurred until a few hours before laying.

5. There was variation in the amount of feeding activity shown in the few hours before oviposition.     

Food,calcium and water intakes by hens lit continuously from hatching

1. Five individually caged Leghorn hens, reared from hatching under continuous light and having their ovipositions uniformly scattered throughout the 24‐h period, were used to study voluntary intakes of a diet low in calcium, oyster shell and water.

2. Food intake was high just after ovulation, decreased during the first 16 h of egg formation, rose transiently 20 to 22 h after ovulation and was minimal just before oviposition.

3. Oyster shell intake was characterised by three peaks, two coincided with those of food diet but the third, occurring between 8 and 12 h after previous oviposition, could be related to the immediate calcium need of shell formation.

4. Water intake followed a similar pattern to food intake but there was an independent rise during albumen plumping (6 to 8 h after previous oviposition).     

A mathematical representation of the Ovulatory cycle of the domestic hen

1. The ovulatory cycle of the domestic hen (Gallus domesticus) was postulated to be the result of the interaction of two independent systems.

2. A circadian system was postulated to control the restriction of ovulation to an 8‐h period of the day under conventional 14 h light: 10 h dark regimes.

3. The final phase of follicular maturation was postulated to commence after ovulation of the preceding ovum in the hierarchy.

4. Ovulation was postulated to occur when a mature follicle was present in the ovary during the appropriate phase of the circadian‐linked system.

5. The predicted times of oviposition were within the standard error of the observed times of oviposition under 21‐, 24‐ and 28‐h photoschedules.

6. It was concluded that this hypothesis for the control of the ovulatory cycle of the hen is consistent with current knowledge.     

Time relationships between oviposition,ovulation and egg formation in khaki campbell ducks

1. Ten‐month‐old Khaki Campbell ducks were killed between 5 min and 15 h after oviposition. Time of oviposition and interval between eggs were recorded prior to killing.

2. Oviposition generally occurred between 04.00 and 06.00 h, 7 to 9 h after the onset of the dark period the previous day. Ninety‐seven percent of eggs were laid by 07.00 h.

3. The mean ± SD time interval between consecutive ovipositions was 24.0 ± 0.3 h, with a range from 23.5 to 24.5 h.

4. It was estimated that ovulation occurred on average 10 min after oviposition, and the ovum spent 15 to 30 min in the infundibulum, 2.5 to 3 h in the magnum, 2 to 2.5 h in the isthmus and 18.6 h in the shell gland.     

Concentrations of plasma luteinising hormone,prolactin, progesterone and androgens during the ovulatory cycle of the Turkey

1. Changes in the concentrations of plasma luteinising hormone (LH), prolactin, androgen and progesterone were measured during the ovulatory cycle of the turkey.

2. Single pre‐ovulatory peaks of plasma LH, androgen and progesterone were observed which took 8, 8 and 12 h respectively, to increase and return to base‐line values. The concentration of plasma prolactin tended to be elevated between 6 h before and 6 h after the LH peak with the maximum values occurring after the peak.

3. The changes in the concentrations of plasma LH and progesterone were 3‐ and 7‐fold respectively while 2‐fold changes were observed in the concentrations of plasma androgen and prolactin.

4. The pre‐ovulatory concentration of plasma progesterone and prolactin began to decrease 4 and 6 h respectively, after the pre‐ovulatory peak of LH.

5. Ovulation and oviposition occurred 6 to 8 h and 36.10+ 0.57 h (SEM) ( n= 11) respectively after the pre‐ovulatory peak of LH.

6. In birds kept on 14 h light/d, pre‐ovulatory peaks of LH were initiated only during a 10 to 11‐h period starting within 2 h after the onset of darkness.

7. A comparison between these data and those from the fowl suggest that the egg is retained in the turkey's oviduct for about 3 to 4 h longer than in the fowl.     

A selection experiment on a white leghorn strain under Ahemeral light‐dark Cycles

1. Selection was carried out for increased ovulation frequency in two flocks; under ahemeral (9 generations of selection) or normal (8 generations) light‐dark cycles.

2. By the use of an ahemeral regime, the barrier to progress imposed by the limit of one ovulation in each light‐dark cycle was evaded.

3. Both flocks achieved a considerable increase in oviposition frequency but so far there has been little evidence of any advantage in this respect due to the ahemeral regime.

4. There was a marked correlated decrease in egg weight in both flocks.     

Modification by metyrapone of the “open period” for pre‐ovulatory LH release in the hen

1. A single injection into laying hens of 60 mg metyrapone 28 h after the final ovulation of a sequence induced increases in the plasma concentrations of LH and progesterone, followed by premature ovulation. Injection of metyrapone 8 h after ovulation, however, did not affect plasma concentrations of either LH or progesterone.

2. Injection of laying hens with 60 mg metyrapone on 5 successive days reduced the effectiveness of exogenous ACTH in increasing the plasma concentration of corticosterone and abolished the system of “ open “ and “ closed periods “ for pre‐ovulatory LH release. Thus, pre‐ovulatory LH surges and ovipositions occurred throughout the 24‐h day instead of being restricted to an 8 to 10‐h period of the day.

3. These observations suggest that changes in environmental stimuli such as light act via the adrenal gland in regulating the timing of the “ open period “ for the pre‐ovulatory release of LH in the hen.     

Effects of egg production on the pattern of food intake of broiler hens kept in continuous light

1. The circadian rhythm of food intake of broiler hens kept in continuous light was more marked when they were laying than it was before they started to lay. It is suggested that this is due to periodicity in oviposition time caused by a regular servicing period.

2. Food intake was reduced for an hour or two before oviposition but increased markedly for a short period immediately afterwards.

3. The rate of food intake decreased about 32 h before oviposition, thereby coinciding with a peak in the release of luteinising hormone, increased at ovulation and increased for several hours after entry of the egg into the uterus.     

Effect of progesterone on ovulation in the hypophysectomised hen

1. One hundred and seven laying hens were hypophysec‐tomised to clarify the relationship between hypophysectomy and the effect of progesterone (P4) on ovulation.

2. Hens were hypophysectomised at 6 intervals from 4.5 to 11.0 h before the expected time of ovulation. Ovulation occurred in the hens operated on from 4.5 to 7.3 h, but did not take place in birds operated on from 9.2 to 9.8 h before the expected time of ovulation.

3. When a single dose of P4 (2 mg/hen) was injected iv immediately after the removal of the anterior pituitary from 7.5 to 9.8 h before the expected time of ovulation, ovulation was induced. However, the percentage of hens responding decreased in proportion to the lapse of time between the hypophysectomy and the expected time of ovulation. No ovulation was induced in hens which were hypophysectomized and given P4 10.2 to 11.0 h before the expected time of ovulation.

4. It is suggested that ovulation is induced by P4 alone possibly in the absence of preovulatory gonadotrophins and that P4 acts directly on the ovary to induce follicle rupture.     

Maturation and ovulation of Japanese quail oocytes under in vitro conditions

1. An in vitro system for ovulation and maturation of Japanese quail oocytes is described.

2. Ovarian follicles removed from the ovary at 2, 4 or 6 h before the estimated time of ovulation may ovulate under in vitro conditions.

3. The presence of progesterone in the medium had a stimulatory effect on the process of maturation, as has been shown for Xenopus oocytes.     

Effect of strain and age on the relationship of oviposition time to shell strength

1. The relationships between oviposition time and specific gravity, shell thickeness, deformation and breaking strength of eggs from six commercial layer strains were examined after 6 and 12 months of production.

2. Shell strength, assessed by any of the methods, was relatively low in eggs collected at 10.00 h and was generally successively greater in those collected at 12.00, 14.00 or 16.00 h. Over the same period egg weight decreased.

3. These time‐related changes in shell strength were similar for the two production ages despite the difference in shell quality due to age.

4. No consistent significant differences were observed between strains in time of oviposition.     

Effect of laying on food and water intake in dwarf and normal hens

1. Irrespective of whether the hen carried the dwarfing gene, dw, food intake on days when there was ovulation and oviposition was higher than on days when there was only oviposition.

2. The “ overconsumption “ in dw hens was greater than in the Dw hens.

3. Food intake and shell thickness were correlated, the relationship being particularly close in dw hens.

4. There was a consistent and positive correlation between food intake and weight of egg laid on the same day in both Dw and dw hens.     

Effect of photoperiod on the mean oviposition time of two breeds of laying hen

1. Oviposition times were recorded for brown and white egg‐laying hybrids under 8, 10, 13 and 18 h photoperiods.

2. Mean oviposition time for both breeds was advanced relative to dusk by approximately 0.5 h for each 1 h extension of photoperiod.

3. Mean oviposition time for the brown egg hybrid was 1.2 to 1.4 h earlier than that of the white egg hybrid under each lighting regimen.

4. A genetic difference in phase setting of the Open Period for Luteinising Hormone (LH) release is the likely reason for the difference in mean time of lay of the two breeds. The difference is possibly one between brown and white hybrids generally, rather than between the particular varieties of hen used in this trial.

5. The proportion of the day in which eggs are laid is shorter under long photoperiods presumably because light at the end of the photoperiod inhibits the pre‐ovulatory surge of LH.     

Effect of progesterone on the magnum proteins during primary stimulation of chick oviduct

1. The effect of progesterone on the secretion of protein by the magnum of 5‐d‐old, female chicks was determined.

2. The supernatant prepared by centrifuging an homogenate of the magnum at 105 000g was found, by immunodiffusion, to contain an antigenic component which precipitated the antisera for conalbumin 1, conalbumin 2 and ovalbumin after 5 d treatment with progesterone: there was no reaction to ovomucoid, lysozyme and avidin antisera.

3. Disc‐electrophoresis of the homogenate revealed two bands at the site of ovalbumin.

4. Incorporation of ³H‐lysine into the magnum proteins of progesterone‐treated chicks did not differ from that of controls.

5. The secretion available in the magnum may be only a transudate from the serum and not a true secretory product. Progesterone behaved qualitatively as oestrogen in this study although the action is much less pronounced and was delayed.     

Genetic and phenotypic parameters for oviposition pattern traits in three selection lines of laying hens

1. Oviposition pattern and egg production traits were recorded in 471 hens from three selection lines. The lines, named according to their selection criteria, were egg number (EN), egg mass (EM) and egg mass, food consumption (EMFC).

2. Mean oviposition intervals within sequences were 24.9, 26.0 and 25.3 h, respectively in the EN, EM and EMFC lines. Corresponding heritability estimates were 0.52, 0.55 and 0.42.

3. The mean oviposition interval within sequences showed a positive genetic correlation with mean oviposition time.

4. Egg weight decreased significantly as the serial number within sequences increased, but only for sequences containing less than 18 eggs.     

Alterations in uterine contractility during the oviposition cycle in domestic hens

1. The potencies of several neurohypophysial hormones were examined at different times during the oviposition cycle in an in vitro fowl oxytocic assay.

2. Uterine tissues were removed 2 h before (—2h‐OP), immediately after (Oh‐OP) and 5 h after (+5h‐OP) spontaneous oviposition. In addition, uterine tissue was removed immediately after oviposition was induced, by administering prostaglandin E₂ 2 h before an expected oviposition (Induced‐OP).

3. The rank order of oxytocic potencies for the peptides was arginine vasotocin=vasopressin>oxytocin>mesotocin. The sensitivity of the uterus to the hormones was Oh‐OP = Induced‐OP> — 2h‐OP= +5‐OP.

4. These results suggest that uterine sensitivity to neurohypophysial hormones changes during the oviposition cycle in domestic fowls.     

Whitening of brown shelled eggs: Individual variation and relationships with age,fearfulness, oviposition interval and stress

1. Fearfulness, shell colour, incidence and degree of shell whitening and the interval between ovipositions were studied in two populations of 30 brown egg laying hens with family histories of a low or a high incidence of egg shell whitening.

2. Hens of the population with the high incidence of whitening appeared to be more fearful than hens of the population with the low incidence of whitening.

3. Brown colouration of the egg shell and the incidence and degree of shell whitening declined as the hens aged.

4. Brown colouration and egg shell whitening were most pronounced on the blunt ends of the eggs.

5. A large part of the variation in egg shell whitening was attributable to the individual (hen) component of variance.

6. Differences in egg shell whitening, between the two populations, were detectable throughout the 26 weeks of the experiment.

7. Oviposition intervals were similar for normal and coated eggs when birds were not exposed to disturbance.

8. Disturbance of hens increased oviposition intervals and the incidence and degree of shell whitening, to a similar extent, in both populations.

9. It is concluded that stress‐related egg retention is not the sole factor responsible for abnormal egg shell whitening. Shell whitening may occur as a consequence of the premature termination of shell pigmentation as well as a consequence of the retardation of oviposition which occurs when hens are disturbed.     

Temporal patterns of ovulation,oviposition and feeding of laying hens under skeleton photoperiods

1. The ovulation, oviposition and eating pattern of laying hens showed that the second light period in the 2L:12D:2L:8D skeleton photo‐period is interpreted as the start of the subjective day.

2. This interpretation is independent of the light period during which the animals are serviced.

3. During each of the 2L periods about one‐third of the total food is eaten, the other third is eaten during the 8D period: little or nothing is eaten during the 12D period.     

Relationships between age,shell quality and individual rate and duration of shell formation in domestic hens

1. The duration and rate of shell formation was measured individually in each of 44 hens. Comparisons were made between hens of different ages, and also between hens which laid eggs of differing shell qualities.

2. Shell formation began 9–5 to 11 h after the oviposition of the previous egg, increased linearly for 13 h and then plateaued at 1–5 h before oviposition.

3. The variation in egg shell quality observed in two experimental groups of hens was 75% attributable to differences in the rate of shell deposition and 25% attributable to difference in the duration of shell deposition.

4. The mean interval between ovipositions increased with age but neither shell weight, nor rate or duration of shell depositon were affected. The increase in egg weight with age accounted for the decrease in shell quality.

5. Intervals between ovipositions were positively correlated with the duration of shell formation, especially its latter stages.

6. Egg production was negatively correlated with the interval between ovipositions but not with the duration or rate of shell deposition. There was a negative correlation between duration and rate of shell deposition, except in the case of the hens producing eggs of poor shell quality.     

Plasma inorganic phosphorus concentration during egg‐shell formation: Effect of the physical form of the dietary calcium

1. Four experiments were carried out with Warren laying hens to elucidate the changes in plasma inorganic phosphorus (P_i) concentration during egg formation.

2. In hens receiving a normal diet containing a calcium supplement in a powdery form P_i increased from 25 to 42 mg/1 during an entire shell formation cycle (from 10 to 22 h after oviposition of the previous egg), while in cockerels P_i decreased slightly during the night.

3. This increase in P_i in hens, was not related to cessation of feeding at the onset of darkness but was specifically connected with the beginning of shell secretion.

4. When hens received calcium as crushed sea‐shells separately from the diet, the nocturnal peak in Pi virtually disappeared and only a temporary increase of 4 mg/1 between 10 and 14 h after oviposition remained.

5. These results indicate that the beginning of shell secretion is always accompanied by an increase in Pi and that a separate presentation of dietary calcium reduces the bone mobilisation at night.